AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019751

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019751 MtrunA17_Chr4g0021261 92.878 337 7 1 1 320 1 337 0.0 639
MsaG019751 MtrunA17_Chr4g0070591 38.667 375 150 14 17 319 1 367 4.38e-60 196
MsaG019751 MtrunA17_Chr2g0300511 39.665 358 144 13 25 319 7 355 9.47e-57 187
MsaG019751 MtrunA17_Chr5g0409471 47.208 197 82 6 25 201 16 210 1.14e-46 160
MsaG019751 MtrunA17_Chr8g0343881 64.706 119 23 3 16 115 1 119 1.87e-44 155
MsaG019751 MtrunA17_Chr1g0152191 55.172 116 30 2 25 118 144 259 1.88e-35 135
MsaG019751 MtrunA17_Chr7g0265931 56.757 111 30 3 26 118 79 189 7.91e-35 130
MsaG019751 MtrunA17_Chr3g0127641 49.593 123 61 1 2 124 90 211 4.13e-32 125
MsaG019751 MtrunA17_Chr8g0351521 45.926 135 66 3 14 144 43 174 1.22e-26 108
MsaG019751 MtrunA17_Chr8g0351521 39.362 94 50 2 46 132 275 368 5.72e-14 72.0
MsaG019751 MtrunA17_Chr8g0351291 64.103 39 14 0 71 109 51 89 2.90e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019751 AT3G13960.1 60.550 109 25 2 25 115 16 124 1.46e-41 149
MsaG019751 AT4G37740.1 50.725 138 44 3 25 138 164 301 7.16e-38 141
MsaG019751 AT2G06200.1 44.118 170 61 4 25 165 6 170 4.13e-36 130
MsaG019751 AT2G06200.2 44.118 170 61 4 25 165 45 209 4.72e-36 131
MsaG019751 AT2G36400.1 51.261 119 37 2 26 123 77 195 2.20e-34 130
MsaG019751 AT3G52910.1 51.786 112 32 3 26 115 83 194 2.10e-31 121
MsaG019751 AT5G53660.1 43.919 148 70 3 11 157 49 184 6.75e-31 119
MsaG019751 AT4G24150.3 41.538 130 54 1 9 116 95 224 5.01e-25 104
MsaG019751 AT4G24150.2 41.538 130 54 1 9 116 133 262 1.01e-24 104
MsaG019751 AT2G06200.3 51.000 100 42 2 68 165 4 98 9.79e-24 95.9
MsaG019751 AT4G24150.1 35.484 155 53 2 9 116 133 287 6.30e-22 96.3
MsaG019751 AT2G45480.2 45.098 102 38 1 29 112 28 129 8.64e-20 88.2
MsaG019751 AT2G45480.1 45.098 102 38 1 29 112 26 127 4.70e-19 87.8
MsaG019751 AT2G45480.1 39.216 102 54 4 30 131 269 362 6.14e-14 72.4
MsaG019751 AT2G45480.3 45.098 102 38 1 29 112 28 129 4.98e-19 87.4
MsaG019751 AT2G45480.3 39.216 102 54 4 30 131 271 364 6.41e-14 72.4

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTTGATCTTTAAATATA+TGG 0.195661 4:+36014214 MsaT019751.1:CDS
CTTAGGCCATTCGCTGAAAA+AGG 0.223874 4:-36017196 None:intergenic
CGCTCACAGTACTTGGAGTC+TGG 0.273319 4:-36015289 None:intergenic
GTCCACCTTCTTCCCATAAA+AGG 0.288851 4:-36015203 None:intergenic
CAATACCTGTATTCTTTACT+TGG 0.293844 4:-36015535 None:intergenic
ATCTTTAAATATATGGTTGC+TGG 0.294888 4:+36014221 MsaT019751.1:CDS
AGCTTCTTTGCAGAAGCTTC+AGG 0.320264 4:+36016973 MsaT019751.1:CDS
TTTCCAGCTGTGACTCCATT+TGG 0.337205 4:-36017014 None:intergenic
TCAGTCCTCCTACATCTTCC+TGG 0.350789 4:-36015232 None:intergenic
GCCTGTGGAATCACAAACTA+TGG 0.360463 4:+36015345 MsaT019751.1:CDS
GTTCAAAATACTACAGGTTC+TGG 0.363646 4:+36015466 MsaT019751.1:CDS
CTCCTACATCTTCCTGGCTC+CGG 0.374741 4:-36015226 None:intergenic
AAGAAGGTGGACCCGGAGCC+AGG 0.384375 4:+36015214 MsaT019751.1:CDS
GAGTGAAAACTGGAATGAAT+AGG 0.396151 4:-36014272 None:intergenic
CAGGCTTTCTTGAACGGTTG+CGG 0.396165 4:-36015327 None:intergenic
CAGAGAGTCATGGTCTGGTT+TGG 0.398433 4:+36017221 MsaT019751.1:CDS
AGGACAAGATCAGGTGGAAC+AGG 0.419490 4:-36014248 None:intergenic
CATTTGTTGTGGGAATGTTC+TGG 0.420078 4:-36015432 None:intergenic
CATCTTGATACCATTCCCTA+TGG 0.426022 4:+36015508 MsaT019751.1:CDS
GACCATGACTCTCTGCTCTT+AGG 0.426877 4:-36017213 None:intergenic
ATTCCCACAACAAATGCATT+TGG 0.432493 4:+36015439 MsaT019751.1:CDS
TCCTACATCTTCCTGGCTCC+GGG 0.435042 4:-36015225 None:intergenic
AATGGTATCAAGATGATAAT+CGG 0.435391 4:-36015500 None:intergenic
CATGTGGCGCTCACAGTACT+TGG 0.436354 4:-36015296 None:intergenic
TTATCTAGTGAATATGGATC+TGG 0.444100 4:+36017138 MsaT019751.1:CDS
GGTAGTGTTCAAAATACTAC+AGG 0.456240 4:+36015460 MsaT019751.1:CDS
CTACCAAATGCATTTGTTGT+GGG 0.462866 4:-36015442 None:intergenic
GCTGGTGGCAGCAGCAGAGC+TGG 0.465144 4:+36015403 MsaT019751.1:CDS
GCCATAGTTTGTGATTCCAC+AGG 0.467528 4:-36015346 None:intergenic
TGTGGAGAATGCTGTTTGGT+TGG 0.482035 4:-36017256 None:intergenic
TTCTTTACTTGGAATCCCAT+AGG 0.487767 4:-36015524 None:intergenic
ATTCCACAGGCTTTCTTGAA+CGG 0.489637 4:-36015333 None:intergenic
TAATTGAACAGGTATCTTCA+AGG 0.495908 4:+36016925 MsaT019751.1:intron
TGAATAGGAAGGACAAGATC+AGG 0.504204 4:-36014257 None:intergenic
AGACAACAAGGGTCTCCAAA+TGG 0.506033 4:+36016999 MsaT019751.1:CDS
TCTCCAAATGGAGTCACAGC+TGG 0.506829 4:+36017011 MsaT019751.1:CDS
GAGTTGTGGAGAATGCTGTT+TGG 0.516076 4:-36017260 None:intergenic
CGGAGGTGTAGGGATGATGA+TGG 0.523373 4:+36014136 MsaT019751.1:CDS
AGTAGTAACAACGGAGGTGT+AGG 0.523982 4:+36014125 MsaT019751.1:CDS
GGATTCCAAGTAAAGAATAC+AGG 0.527798 4:+36015530 MsaT019751.1:CDS
TCTTTACTTGGAATCCCATA+GGG 0.529110 4:-36015523 None:intergenic
GGAAGATGTAGGAGGACTGA+TGG 0.529508 4:+36015235 MsaT019751.1:CDS
TTATGGGAAGAAGGTGGACC+CGG 0.531013 4:+36015207 MsaT019751.1:CDS
TGGAGAAGCTGTGAAGGAAG+AGG 0.531350 4:+36017158 MsaT019751.1:CDS
AAGAGCAGAGAGTCATGGTC+TGG 0.532638 4:+36017216 MsaT019751.1:CDS
ACTACCAAATGCATTTGTTG+TGG 0.537213 4:-36015443 None:intergenic
CTTCACAGTGTCTCAGTGGC+AGG 0.545162 4:+36014178 MsaT019751.1:CDS
GGGATAATCACTTTGCAACA+TGG 0.547856 4:-36017103 None:intergenic
ACTTGGAATCCCATAGGGAA+TGG 0.549125 4:-36015518 None:intergenic
AGCTTCAGGAGGAGACAACA+AGG 0.549332 4:+36016987 MsaT019751.1:CDS
GAGAAGTCAGACGATGACAT+AGG 0.553675 4:-36017300 None:intergenic
GTCAGACGATGACATAGGAA+TGG 0.557702 4:-36017295 None:intergenic
GCTCACAGTACTTGGAGTCT+GGG 0.561352 4:-36015288 None:intergenic
ATCTTGATACCATTCCCTAT+GGG 0.573222 4:+36015509 MsaT019751.1:CDS
TCTTCAAGGTCTTAAATCTG+AGG 0.573656 4:+36016939 MsaT019751.1:CDS
TGGATCTGGAGAAGCTGTGA+AGG 0.577779 4:+36017152 MsaT019751.1:CDS
GGAGAAGCTGTGAAGGAAGA+GGG 0.582664 4:+36017159 MsaT019751.1:CDS
GTAGTAACAACGGAGGTGTA+GGG 0.587459 4:+36014126 MsaT019751.1:CDS
TTCTTTGCAGAAGCTTCAGG+AGG 0.588543 4:+36016976 MsaT019751.1:CDS
GGATATTGAAAGTTGAGTTG+TGG 0.598447 4:-36017274 None:intergenic
GTGACATCGCTAACTGTCGC+TGG 0.600432 4:+36015385 MsaT019751.1:CDS
CTTCAAGGTCTTAAATCTGA+GGG 0.600678 4:+36016940 MsaT019751.1:CDS
AAAAGTATGAATAGCTCTGG+TGG 0.606971 4:+36014098 None:intergenic
GAAAACTGGAATGAATAGGA+AGG 0.611240 4:-36014268 None:intergenic
CACCCTTCACAGTGTCTCAG+TGG 0.621198 4:+36014174 MsaT019751.1:CDS
GGTTGCGGCCACGATGCATG+TGG 0.624950 4:-36015312 None:intergenic
GGCCTAAGAGCAGAGAGTCA+TGG 0.630587 4:+36017211 MsaT019751.1:CDS
AGTATGAATAGCTCTGGTGG+TGG 0.634205 4:+36014101 None:intergenic
CAACCGTTCAAGAAAGCCTG+TGG 0.634486 4:+36015330 MsaT019751.1:CDS
GCAACTCTAGTTGACATCAA+AGG 0.643622 4:-36017045 None:intergenic
CGGAGCCAGGAAGATGTAGG+AGG 0.644072 4:+36015227 MsaT019751.1:CDS
ACCCGGAGCCAGGAAGATGT+AGG 0.647650 4:+36015224 MsaT019751.1:CDS
TGCCACTGAGACACTGTGAA+GGG 0.647925 4:-36014176 None:intergenic
GGAGGTGTAGGGATGATGAT+GGG 0.651275 4:+36014137 MsaT019751.1:CDS
ACAAGCTCTCAGTCGCCACA+CGG 0.652578 4:+36017327 MsaT019751.1:CDS
ATAGGAAGGACAAGATCAGG+TGG 0.667033 4:-36014254 None:intergenic
CTGCCACTGAGACACTGTGA+AGG 0.667964 4:-36014177 None:intergenic
ACATCGCTAACTGTCGCTGG+TGG 0.682168 4:+36015388 MsaT019751.1:CDS
AGTCACAGCTGGAAAACACA+TGG 0.689809 4:+36017022 MsaT019751.1:CDS
CAAGGTCTTAAATCTGAGGG+TGG 0.691896 4:+36016943 MsaT019751.1:CDS
GCTTCAGGAGGAGACAACAA+GGG 0.694886 4:+36016988 MsaT019751.1:CDS
TATCAAGATGATAATCGGCA+CGG 0.696821 4:-36015495 None:intergenic
GGTGGTGGCAGTAGTAACAA+CGG 0.699810 4:+36014116 MsaT019751.1:CDS
TGTGAGCGCCACATGCATCG+TGG 0.717232 4:+36015304 MsaT019751.1:CDS
GACAGTTAGCGATGTCACAG+TGG 0.746561 4:-36015380 None:intergenic
GGTGGCAGTAGTAACAACGG+AGG 0.768714 4:+36014119 MsaT019751.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 36014104 36017440 36014104 ID=MsaG019751
Chr4 mRNA 36014104 36017440 36014104 ID=MsaT019751.1;Parent=MsaG019751
Chr4 exon 36014104 36014284 36014104 ID=MsaT019751.1.exon1;Parent=MsaT019751.1
Chr4 CDS 36014104 36014284 36014104 ID=cds.MsaT019751.1;Parent=MsaT019751.1
Chr4 exon 36015191 36015551 36015191 ID=MsaT019751.1.exon2;Parent=MsaT019751.1
Chr4 CDS 36015191 36015551 36015191 ID=cds.MsaT019751.1;Parent=MsaT019751.1
Chr4 exon 36016936 36017348 36016936 ID=MsaT019751.1.exon3;Parent=MsaT019751.1
Chr4 CDS 36016936 36017348 36016936 ID=cds.MsaT019751.1;Parent=MsaT019751.1
Chr4 exon 36017433 36017440 36017433 ID=MsaT019751.1.exon4;Parent=MsaT019751.1
Chr4 CDS 36017433 36017440 36017433 ID=cds.MsaT019751.1;Parent=MsaT019751.1
Gene Sequence

>MsaT019751.1

ATGAATAGCTCTGGTGGTGGCAGTAGTAACAACGGAGGTGTAGGGATGATGATGGGTTACAGTAAATCATCACCCTTCACAGTGTCTCAGTGGCAGGAACTTGAACATCAAGCTTTGATCTTTAAATATATGGTTGCTGGTCTTCCTGTTCCACCTGATCTTGTCCTTCCTATTCATTCCATGAGTTACTGTTCCTTTTATGGGAAGAAGGTGGACCCGGAGCCAGGAAGATGTAGGAGGACTGATGGAAAAAAGTGGAGGTGCTCTAAGGAAGCATACCCAGACTCCAAGTACTGTGAGCGCCACATGCATCGTGGCCGCAACCGTTCAAGAAAGCCTGTGGAATCACAAACTATGGCACAGTCGTCATCCACTGTGACATCGCTAACTGTCGCTGGTGGCAGCAGCAGAGCTGGTAACTTCCAGAACATTCCCACAACAAATGCATTTGGTAGTGTTCAAAATACTACAGGTTCTGGAACTGACCGTGCCGATTATCATCTTGATACCATTCCCTATGGGATTCCAAGTAAAGAATACAGGTATCTTCAAGGTCTTAAATCTGAGGGTGGAGAACATAGCTTCTTTGCAGAAGCTTCAGGAGGAGACAACAAGGGTCTCCAAATGGAGTCACAGCTGGAAAACACATGGCCTTTGATGTCAACTAGAGTTGCCTCTTTTTCTGCATCAAAATCAAGCTGTAATAATTCCATGTTGCAAAGTGATTATCCCCAACATTCATTTTTATCTAGTGAATATGGATCTGGAGAAGCTGTGAAGGAAGAGGGCCAGCCTCTTCGACCCTTTTTCAGCGAATGGCCTAAGAGCAGAGAGTCATGGTCTGGTTTGGAAGATGAGAGATCCAACCAAACAGCATTCTCCACAACTCAACTTTCAATATCCATTCCTATGTCATCGTCTGACTTCTCTGCTACAAGCTCTCAGTCGCCACACGATAACTAG
Protein sequence

>MsaT019751.1

MNSSGGGSSNNGGVGMMMGYSKSSPFTVSQWQELEHQALIFKYMVAGLPVPPDLVLPIHSMSYCSFYGKKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVESQTMAQSSSTVTSLTVAGGSSRAGNFQNIPTTNAFGSVQNTTGSGTDRADYHLDTIPYGIPSKEYRYLQGLKSEGGEHSFFAEASGGDNKGLQMESQLENTWPLMSTRVASFSASKSSCNNSMLQSDYPQHSFLSSEYGSGEAVKEEGQPLRPFFSEWPKSRESWSGLEDERSNQTAFSTTQLSISIPMSSSDFSATSSQSPHDN*