Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019875 | KEH15779.1 | 88.672 | 256 | 28 | 1 | 1 | 255 | 1 | 256 | 5.22e-167 | 473 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019875 | sp|Q9SZL8|FRS5_ARATH | 26.364 | 220 | 145 | 4 | 32 | 251 | 70 | 272 | 4.85e-14 | 74.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019875 | tr|A0A072TE13|A0A072TE13_MEDTR | 88.672 | 256 | 28 | 1 | 1 | 255 | 1 | 256 | 2.49e-167 | 473 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG019875 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG002029 | MsaG019875 | 0.832617 | 1.544418e-55 | 4.821011e-53 |
| MsaG002030 | MsaG019875 | 0.828166 | 1.869460e-54 | 5.136221e-52 |
| MsaG010290 | MsaG019875 | 0.809306 | 3.447008e-50 | 5.788408e-48 |
| MsaG017337 | MsaG019875 | 0.805022 | 2.756667e-49 | 4.181539e-47 |
| MsaG019875 | MsaG025574 | 0.810024 | 2.420096e-50 | 4.135184e-48 |
| MsaG019875 | MsaG032147 | 0.805848 | 1.853140e-49 | 2.865596e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019875 | MtrunA17_Chr1g0172971 | 82.051 | 117 | 20 | 1 | 1 | 116 | 1 | 117 | 1.46e-59 | 197 |
| MsaG019875 | MtrunA17_Chr6g0475591 | 94.118 | 68 | 4 | 0 | 188 | 255 | 1 | 68 | 8.98e-42 | 137 |
| MsaG019875 | MtrunA17_Chr7g0258861 | 27.477 | 222 | 139 | 5 | 36 | 254 | 8 | 210 | 2.57e-22 | 93.6 |
| MsaG019875 | MtrunA17_Chr1g0202341 | 30.493 | 223 | 131 | 6 | 34 | 254 | 50 | 250 | 2.03e-21 | 93.2 |
| MsaG019875 | MtrunA17_Chr2g0324011 | 26.126 | 222 | 141 | 5 | 36 | 254 | 8 | 209 | 3.80e-18 | 83.6 |
| MsaG019875 | MtrunA17_Chr5g0448761 | 24.324 | 222 | 152 | 3 | 32 | 253 | 57 | 262 | 1.72e-13 | 69.7 |
| MsaG019875 | MtrunA17_Chr8g0363841 | 26.728 | 217 | 134 | 7 | 40 | 253 | 22 | 216 | 1.22e-12 | 67.4 |
| MsaG019875 | MtrunA17_Chr3g0082121 | 25.234 | 214 | 133 | 6 | 41 | 254 | 49 | 235 | 2.02e-12 | 66.6 |
| MsaG019875 | MtrunA17_Chr2g0296781 | 25.806 | 217 | 133 | 6 | 39 | 253 | 1 | 191 | 1.75e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG019875 | AT4G38180.1 | 26.364 | 220 | 145 | 4 | 32 | 251 | 70 | 272 | 4.93e-15 | 74.7 |
Find 38 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 4:-39314235 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 4:-39313996 | None:intergenic |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 4:+39314006 | MsaT019875.1:CDS |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 4:+39314077 | MsaT019875.1:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 4:+39314412 | MsaT019875.1:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 4:+39314294 | MsaT019875.1:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 4:+39314326 | MsaT019875.1:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 4:+39314277 | MsaT019875.1:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 4:+39314160 | MsaT019875.1:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 4:+39314442 | MsaT019875.1:CDS |
| ATCTGAGAGTGGACAATTGA+CGG | 0.452529 | 4:+39313931 | MsaT019875.1:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 4:-39314382 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 4:+39314126 | MsaT019875.1:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 4:+39314487 | MsaT019875.1:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 4:-39314271 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 4:+39314064 | MsaT019875.1:CDS |
| TCTAATGACAACTCGTCAAA+AGG | 0.488137 | 4:+39314555 | MsaT019875.1:CDS |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 4:+39314120 | MsaT019875.1:CDS |
| CGTCAAAAGGAGATGGTGGC+TGG | 0.492100 | 4:+39314568 | MsaT019875.1:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 4:+39314474 | MsaT019875.1:CDS |
| TGTCTTATGCAGCATCAACT+TGG | 0.503558 | 4:-39314660 | None:intergenic |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 4:-39314526 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 4:+39314330 | MsaT019875.1:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 4:+39314171 | MsaT019875.1:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 4:+39313996 | MsaT019875.1:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 4:+39314172 | MsaT019875.1:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 4:-39314370 | None:intergenic |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 4:+39313955 | MsaT019875.1:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 4:+39314490 | MsaT019875.1:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 4:+39314481 | MsaT019875.1:CDS |
| GACAACTCGTCAAAAGGAGA+TGG | 0.599038 | 4:+39314561 | MsaT019875.1:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 4:+39314475 | MsaT019875.1:CDS |
| AACTCGTCAAAAGGAGATGG+TGG | 0.603142 | 4:+39314564 | MsaT019875.1:CDS |
| ATATCAACTTCATCTGAGAG+TGG | 0.614980 | 4:+39313920 | MsaT019875.1:CDS |
| AATGAAGCTCTCTCAAAACC+AGG | 0.618452 | 4:+39314622 | MsaT019875.1:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 4:-39314220 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 4:+39314495 | MsaT019875.1:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 4:+39314329 | MsaT019875.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 39313911 | 39314678 | 39313911 | ID=MsaG019875 |
| Chr4 | mRNA | 39313911 | 39314678 | 39313911 | ID=MsaT019875.1;Parent=MsaG019875 |
| Chr4 | exon | 39313911 | 39314678 | 39313911 | ID=MsaT019875.1.exon1;Parent=MsaT019875.1 |
| Chr4 | CDS | 39313911 | 39314678 | 39313911 | ID=cds.MsaT019875.1;Parent=MsaT019875.1 |
| Gene Sequence |
| Protein sequence |
