AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019889


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019889 MtrunA17_Chr8g0352871 85.806 155 14 1 1 147 1 155 7.32e-94 269
MsaG019889 MtrunA17_Chr3g0082861 74.839 155 30 1 7 152 2 156 2.61e-82 239
MsaG019889 MtrunA17_Chr7g0256551 69.427 157 39 1 1 148 1 157 6.78e-79 233
MsaG019889 MtrunA17_Chr4g0021731 96.512 86 3 0 1 86 3 88 1.74e-56 171
MsaG019889 MtrunA17_Chr3g0124861 54.717 159 53 3 9 149 22 179 9.64e-56 174
MsaG019889 MtrunA17_Chr4g0059571 58.042 143 60 0 4 146 21 163 1.08e-53 168
MsaG019889 MtrunA17_Chr3g0128691 60.305 131 48 2 10 137 19 148 1.87e-51 161
MsaG019889 MtrunA17_Chr5g0414931 55.479 146 59 2 8 148 15 159 3.44e-51 163
MsaG019889 MtrunA17_Chr3g0118211 51.678 149 72 0 4 152 18 166 4.05e-51 161
MsaG019889 MtrunA17_Chr1g0170741 59.854 137 52 2 8 141 7 143 5.05e-50 159
MsaG019889 MtrunA17_Chr4g0030271 29.545 132 84 2 7 138 87 209 3.61e-15 71.6
MsaG019889 MtrunA17_Chr8g0345901 29.008 131 84 1 11 141 38 159 3.66e-15 71.6
MsaG019889 MtrunA17_Chr8g0376881 30.769 143 87 3 4 146 24 154 6.90e-15 70.9
MsaG019889 MtrunA17_Chr1g0181811 28.125 128 83 2 11 138 87 205 2.58e-13 66.2
MsaG019889 MtrunA17_Chr7g0275891 31.429 140 83 4 11 150 11 137 3.60e-13 65.5
MsaG019889 MtrunA17_Chr1g0149841 29.286 140 85 3 11 150 11 136 1.18e-12 64.3
MsaG019889 MtrunA17_Chr3g0123131 28.676 136 85 2 11 146 19 142 1.21e-12 64.3
MsaG019889 MtrunA17_Chr1g0161431 30.216 139 85 3 9 147 15 141 1.44e-12 63.9
MsaG019889 MtrunA17_Chr1g0149851 31.538 130 77 3 11 140 11 128 3.11e-12 63.2
MsaG019889 MtrunA17_Chr7g0275401 30.303 132 80 3 9 140 9 128 3.28e-12 62.4
MsaG019889 MtrunA17_Chr7g0276361 26.515 132 88 1 7 138 12 134 1.59e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019889 AT3G57040.2 67.742 155 40 1 1 145 1 155 1.23e-73 220
MsaG019889 AT3G57040.1 67.742 155 40 1 1 145 1 155 1.23e-73 220
MsaG019889 AT2G41310.1 63.871 155 48 1 1 147 1 155 1.01e-70 213
MsaG019889 AT1G59940.1 61.832 131 50 0 8 138 32 162 1.45e-55 174
MsaG019889 AT1G59940.2 61.832 131 50 0 8 138 124 254 5.10e-54 173
MsaG019889 AT3G48100.1 58.273 139 57 1 2 139 18 156 6.34e-54 168
MsaG019889 AT1G10470.3 60.305 131 52 0 8 138 19 149 7.62e-54 170
MsaG019889 AT1G10470.1 60.305 131 52 0 8 138 33 163 9.57e-54 171
MsaG019889 AT5G62920.1 61.538 130 50 0 9 138 25 154 2.17e-52 164
MsaG019889 AT1G19050.1 55.147 136 61 0 8 143 23 158 5.64e-52 164
MsaG019889 AT1G74890.1 56.934 137 59 0 8 144 17 153 9.93e-52 164
MsaG019889 AT3G56380.1 53.623 138 63 1 4 140 15 152 4.96e-51 160
MsaG019889 AT3G56380.2 53.237 139 63 2 4 140 15 153 3.18e-49 155
MsaG019889 AT2G40670.1 52.518 139 57 2 8 140 28 163 4.88e-49 155
MsaG019889 AT2G40670.2 52.143 140 57 3 8 140 28 164 3.43e-47 150
MsaG019889 AT2G07440.1 65.574 61 21 0 87 147 9 69 8.31e-23 87.8
MsaG019889 AT1G10470.2 58.491 53 22 0 86 138 35 87 3.39e-16 72.0
MsaG019889 AT2G25180.1 32.308 130 76 3 11 140 19 136 8.84e-14 67.8
MsaG019889 AT5G07210.2 28.472 144 90 3 8 151 15 145 1.96e-13 67.0
MsaG019889 AT5G60100.7 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
MsaG019889 AT5G60100.6 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
MsaG019889 AT5G60100.4 30.469 128 80 2 11 138 66 184 6.58e-13 65.5
MsaG019889 AT5G60100.2 30.469 128 80 2 11 138 66 184 8.13e-13 65.1
MsaG019889 AT5G02810.1 27.344 128 84 2 11 138 80 198 9.49e-13 65.1
MsaG019889 AT1G67710.1 28.148 135 85 2 9 143 11 133 1.11e-12 64.7
MsaG019889 AT5G60100.5 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
MsaG019889 AT5G60100.3 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
MsaG019889 AT5G60100.1 30.469 128 80 2 11 138 66 184 1.50e-12 64.3
MsaG019889 AT3G16857.1 28.889 135 84 3 9 143 37 159 1.03e-11 62.0
MsaG019889 AT3G16857.2 28.889 135 84 3 9 143 37 159 1.04e-11 62.0
MsaG019889 AT2G46790.3 25.170 147 100 3 6 152 34 170 1.99e-11 61.2
MsaG019889 AT2G46790.1 25.170 147 100 3 6 152 34 170 2.01e-11 61.2
MsaG019889 AT1G49190.1 28.788 132 82 3 10 141 35 154 3.28e-11 60.5
MsaG019889 AT5G24470.1 27.820 133 85 2 9 140 50 172 5.42e-11 60.1

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTATGTCTTTAAAAGATT+TGG 0.160260 4:-39821741 None:intergenic
CTGATTGTTGAACTGGTTTC+AGG 0.173333 4:-39821979 None:intergenic
AGAGACTCAGTTCCATGTTT+TGG 0.251951 4:+39820863 MsaT019889.1:CDS
AGTAAGGCTTTAAAATTTCT+TGG 0.266953 4:+39821148 MsaT019889.1:CDS
TCTCACCTGCTAATCCTTGA+TGG 0.304860 4:-39821791 None:intergenic
GGCTTTAAAATTTCTTGGTT+TGG 0.308229 4:+39821153 MsaT019889.1:CDS
TTTACATCTGATTGTTGAAC+TGG 0.343425 4:-39821986 None:intergenic
TCTTGGTTTGGTTGAGGATG+TGG 0.414547 4:+39821165 MsaT019889.1:CDS
TTTGATGAAATCAAGAGTTA+AGG 0.416978 4:+39822024 MsaT019889.1:CDS
TACAATGGACTCTGCTAGTA+AGG 0.427203 4:+39821132 MsaT019889.1:CDS
TATTGTATGCCAGGGATGAC+TGG 0.449232 4:+39821331 MsaT019889.1:CDS
AAAATTTCTTGGTTTGGTTG+AGG 0.449963 4:+39821159 MsaT019889.1:CDS
ACTCTTGATTTCATCAAATG+AGG 0.455768 4:-39822019 None:intergenic
ATAACAGATTATTGTATGCC+AGG 0.463952 4:+39821322 MsaT019889.1:CDS
ATGTTCCATCAAGGATTAGC+AGG 0.504757 4:+39821786 MsaT019889.1:CDS
TTGATAGAATGTTGATTGAG+AGG 0.516155 4:+39820904 MsaT019889.1:CDS
GATGTGGCTTCAGAAGTTCA+TGG 0.523538 4:+39821181 MsaT019889.1:CDS
ATGTGGCTTCAGAAGTTCAT+GGG 0.534908 4:+39821182 MsaT019889.1:CDS
CTTCAGAGAATGTTCCATCA+AGG 0.539392 4:+39821777 MsaT019889.1:CDS
AGATCATAGCCAGTCATCCC+TGG 0.546401 4:-39821340 None:intergenic
TCTGAAGACATAATCACAAT+CGG 0.548679 4:-39821761 None:intergenic
TAACAGATTATTGTATGCCA+GGG 0.564090 4:+39821323 MsaT019889.1:CDS
TATCATCAACAGCCAAAACA+TGG 0.580138 4:-39820875 None:intergenic
TGAAATGATAGTTACTACAA+TGG 0.624009 4:+39821117 MsaT019889.1:intron
TGTGGCTTCAGAAGTTCATG+GGG 0.695893 4:+39821183 MsaT019889.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 39820849 39822078 39820849 ID=MsaG019889
Chr4 mRNA 39820849 39822078 39820849 ID=MsaT019889.1;Parent=MsaG019889
Chr4 exon 39820849 39820951 39820849 ID=MsaT019889.1.exon1;Parent=MsaT019889.1
Chr4 CDS 39820849 39820951 39820849 ID=cds.MsaT019889.1;Parent=MsaT019889.1
Chr4 exon 39821128 39821204 39821128 ID=MsaT019889.1.exon2;Parent=MsaT019889.1
Chr4 CDS 39821128 39821204 39821128 ID=cds.MsaT019889.1;Parent=MsaT019889.1
Chr4 exon 39821301 39821378 39821301 ID=MsaT019889.1.exon3;Parent=MsaT019889.1
Chr4 CDS 39821301 39821378 39821301 ID=cds.MsaT019889.1;Parent=MsaT019889.1
Chr4 exon 39821737 39821807 39821737 ID=MsaT019889.1.exon4;Parent=MsaT019889.1
Chr4 CDS 39821737 39821807 39821737 ID=cds.MsaT019889.1;Parent=MsaT019889.1
Chr4 exon 39821949 39822078 39821949 ID=MsaT019889.1.exon5;Parent=MsaT019889.1
Chr4 CDS 39821949 39822078 39821949 ID=cds.MsaT019889.1;Parent=MsaT019889.1
Gene Sequence

>MsaT019889.1

ATGGGTATGGCTGCAGAGACTCAGTTCCATGTTTTGGCTGTTGATGATAGTATTATTGATAGAATGTTGATTGAGAGGCTCCTCAAAACTTCTTCTTTTCATGTTACTACAATGGACTCTGCTAGTAAGGCTTTAAAATTTCTTGGTTTGGTTGAGGATGTGGCTTCAGAAGTTCATGGGGATGTAGATGTAAATCTGATCATAACAGATTATTGTATGCCAGGGATGACTGGCTATGATCTGCTCAGAAAAATCAAGGAATCCAAATCTTTTAAAGACATACCGATTGTGATTATGTCTTCAGAGAATGTTCCATCAAGGATTAGCAGATGTTTTGAAGAGGGAGCTGAAGAATTCTTCCTGAAACCAGTTCAACAATCAGATGTAAACAAGCTGAAGCCTCATTTGATGAAATCAAGAGTTAAGGAAGAGCAAGAACAAACAACCCATCAACAATAA

Protein sequence

>MsaT019889.1

MGMAAETQFHVLAVDDSIIDRMLIERLLKTSSFHVTTMDSASKALKFLGLVEDVASEVHGDVDVNLIITDYCMPGMTGYDLLRKIKESKSFKDIPIVIMSSENVPSRISRCFEEGAEEFFLKPVQQSDVNKLKPHLMKSRVKEEQEQTTHQQ*