AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019908

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019908 MtrunA17_Chr4g0025521 95.781 711 29 1 1 710 1 711 0.0 1321
MsaG019908 MtrunA17_Chr7g0267481 45.087 743 292 26 1 710 1 660 2.10e-165 491
MsaG019908 MtrunA17_Chr1g0177751 46.122 735 285 28 1 710 1 649 1.07e-162 483
MsaG019908 MtrunA17_Chr3g0141941 44.893 421 173 15 90 492 56 435 2.63e-95 305
MsaG019908 MtrunA17_Chr1g0155681 58.052 267 97 4 218 476 172 431 4.41e-95 307
MsaG019908 MtrunA17_Chr8g0373801 50.890 281 131 3 216 490 388 667 2.34e-83 281
MsaG019908 MtrunA17_Chr5g0403251 51.273 275 126 4 206 476 329 599 3.24e-78 265
MsaG019908 MtrunA17_Chr8g0387321 52.708 277 118 3 216 486 185 454 4.54e-78 262
MsaG019908 MtrunA17_Chr3g0141931 45.172 290 141 4 209 490 158 437 1.28e-71 239
MsaG019908 MtrunA17_Chr4g0028701 39.506 324 144 5 216 493 245 562 9.52e-64 224
MsaG019908 MtrunA17_Chr4g0008591 42.908 282 124 4 216 489 196 448 5.84e-62 218
MsaG019908 MtrunA17_Chr7g0240371 43.590 273 133 6 216 484 137 392 5.02e-60 210
MsaG019908 MtrunA17_Chr2g0327021 38.854 314 163 7 216 508 248 553 4.38e-59 210
MsaG019908 MtrunA17_Chr1g0151681 36.667 330 169 9 179 480 186 503 2.59e-52 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019908 AT2G35940.3 42.649 755 313 27 1 710 1 680 2.43e-133 409
MsaG019908 AT2G35940.1 42.649 755 313 27 1 710 1 680 2.43e-133 409
MsaG019908 AT2G35940.2 42.649 755 313 27 1 710 1 680 2.43e-133 409
MsaG019908 AT2G16400.1 51.149 348 136 8 149 491 57 375 4.22e-97 308
MsaG019908 AT4G34610.2 50.568 352 143 10 175 516 94 424 1.75e-95 306
MsaG019908 AT4G34610.4 50.568 352 143 10 175 516 94 424 1.75e-95 306
MsaG019908 AT4G34610.3 50.568 352 143 10 175 516 94 424 1.75e-95 306
MsaG019908 AT4G34610.1 50.568 352 143 10 175 516 94 424 1.75e-95 306
MsaG019908 AT1G75410.1 53.310 287 111 7 205 482 160 432 3.46e-88 286
MsaG019908 AT1G75410.2 53.310 287 111 7 205 482 160 432 3.46e-88 286
MsaG019908 AT1G19700.1 53.069 277 99 6 217 477 166 427 2.10e-87 285
MsaG019908 AT1G19700.3 53.069 277 99 6 217 477 166 427 2.10e-87 285
MsaG019908 AT1G19700.5 53.069 277 99 6 217 477 166 427 2.10e-87 285
MsaG019908 AT1G19700.4 53.069 277 99 6 217 477 166 427 2.10e-87 285
MsaG019908 AT1G19700.2 53.069 277 99 6 217 477 166 427 2.10e-87 285
MsaG019908 AT2G27220.1 46.648 358 144 9 146 501 12 324 4.04e-86 278
MsaG019908 AT2G27220.3 46.648 358 144 9 146 501 12 324 4.04e-86 278
MsaG019908 AT2G27220.2 44.415 376 144 10 146 501 12 342 4.35e-82 268
MsaG019908 AT2G23760.2 52.809 267 112 3 208 468 233 491 2.52e-80 268
MsaG019908 AT2G23760.1 52.809 267 112 3 208 468 233 491 2.52e-80 268
MsaG019908 AT2G23760.3 52.809 267 112 3 208 468 233 491 2.52e-80 268
MsaG019908 AT4G36870.2 51.351 259 115 3 216 468 312 565 1.37e-78 266
MsaG019908 AT4G36870.1 51.351 259 115 3 216 468 312 565 1.37e-78 266
MsaG019908 AT2G23760.4 44.201 319 112 4 208 468 233 543 1.47e-70 243
MsaG019908 AT1G75410.3 50.973 257 90 7 205 448 160 393 1.73e-70 236
MsaG019908 AT5G41410.1 46.014 276 130 3 217 476 194 466 1.71e-68 236
MsaG019908 AT4G36870.4 47.500 240 115 3 216 449 312 546 6.35e-63 219
MsaG019908 AT4G36870.3 47.059 238 115 3 216 447 312 544 9.71e-62 217
MsaG019908 AT1G75430.1 40.484 289 146 8 210 481 10 289 2.06e-60 205
MsaG019908 AT5G02030.1 41.137 299 123 4 197 472 151 419 1.14e-59 211
MsaG019908 AT4G32980.1 37.460 315 165 7 177 478 154 449 1.28e-51 186
MsaG019908 AT4G32980.2 37.460 315 165 7 177 478 154 449 1.28e-51 186
MsaG019908 AT2G27990.1 38.562 306 152 8 174 473 221 496 2.12e-49 183
MsaG019908 AT1G75430.2 35.526 228 128 7 210 427 10 228 6.64e-32 126
MsaG019908 AT2G27990.2 30.116 259 147 7 174 427 221 450 2.02e-23 104

Find 181 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTTGACATTGAATTAT+TGG 0.252974 4:+40739938 None:intergenic
ATTCCACCTTCCAACTTTCA+TGG 0.270274 4:-40744315 MsaT019908.1:CDS
CTCTCTCAGAATTTACATTT+AGG 0.274546 4:-40739540 MsaT019908.1:CDS
GGGTCCTTTCAATCCAATTC+TGG 0.288442 4:-40739897 MsaT019908.1:CDS
CTGCTTTGAGATTGTCTTCA+AGG 0.297342 4:+40741979 None:intergenic
AATTCTGGTTTCCATCTTGC+AGG 0.297418 4:-40739882 MsaT019908.1:CDS
GTGTTAAGTTAGAAGGTTCT+AGG 0.298713 4:-40741880 MsaT019908.1:CDS
TCTTGCAAGCATAACCTTAT+CGG 0.300640 4:+40740945 None:intergenic
ACAAATAGATCAAAAGAATT+AGG 0.307786 4:-40740050 MsaT019908.1:CDS
TCACTGTTGGAGAAAGGTTT+TGG 0.308937 4:+40739970 None:intergenic
CTTCGCAACAAACGCCTTAC+CGG 0.309937 4:-40744029 MsaT019908.1:CDS
AGGAGGGACGATTGATGAAA+TGG 0.313418 4:-40739520 MsaT019908.1:CDS
TTTGAGCAAGCAACAGGATA+TGG 0.320173 4:-40742028 MsaT019908.1:CDS
TGCTTGGGTGTTAAGTTAGA+AGG 0.330959 4:-40741887 MsaT019908.1:CDS
TCAGAGTAAGGTACGTAGTT+TGG 0.340254 4:+40744483 None:intergenic
GGTTCTCCTCACGCGCTTCA+TGG 0.341873 4:-40744396 MsaT019908.1:CDS
GCCGAAAATAACACCGTTCA+TGG 0.355276 4:+40743939 None:intergenic
TCAAGGTAGTTTCCTTTCAA+TGG 0.367613 4:-40739823 MsaT019908.1:CDS
GAGTTTCGCCCGCAGCAAAT+TGG 0.368022 4:-40744096 MsaT019908.1:CDS
GACAAACCACGCTGATAAAC+TGG 0.368350 4:+40744063 None:intergenic
AATCATCATGGTGGTGGTTA+TGG 0.369811 4:-40739702 MsaT019908.1:CDS
GTTATGCTTGCAAGACAAAC+TGG 0.371730 4:-40740937 MsaT019908.1:CDS
TGATCTATTTGTGTTATCCT+TGG 0.372128 4:+40740061 None:intergenic
AGAACAGAAACAGCTCTTTC+AGG 0.372428 4:+40741764 None:intergenic
GGAGGGACGATTGATGAAAT+GGG 0.373488 4:-40739519 MsaT019908.1:CDS
CAAGACAAACTGGACTTACT+AGG 0.381649 4:-40740927 MsaT019908.1:CDS
TCTCAATGGAAGATGTTAGA+AGG 0.381842 4:-40739674 MsaT019908.1:CDS
ATATCACACTTTGCATTTGA+TGG 0.388198 4:+40742056 None:intergenic
CTGTTTCTGTTCTTCGTGCT+TGG 0.388531 4:-40741754 MsaT019908.1:CDS
TTTCATTTCCACTCAATAAA+GGG 0.390624 4:+40739724 None:intergenic
GAAATCCAACCGTCGGTAGA+AGG 0.392044 4:-40744531 MsaT019908.1:CDS
ACGGTAGCCGAGGAGGAAAA+TGG 0.396762 4:+40744240 None:intergenic
GTTGCTGTGACAGCGTTATC+AGG 0.403199 4:+40744171 None:intergenic
TAAGACAGCTAGCAAGACTT+TGG 0.403443 4:-40741921 MsaT019908.1:CDS
CACTATAACATGTGGGGATC+GGG 0.405577 4:-40744204 MsaT019908.1:CDS
CATAACCTTATCGGAGTCCT+TGG 0.408384 4:+40740954 None:intergenic
CCGATCCCCACATGTTATAG+TGG 0.411001 4:+40744205 None:intergenic
TGCAAGATGGAAACCAGAAT+TGG 0.412205 4:+40739884 None:intergenic
GATATGGCAACGTACTTTCA+TGG 0.416361 4:-40744576 MsaT019908.1:CDS
AACGCTACCACTAACAATAA+TGG 0.417083 4:-40744417 MsaT019908.1:CDS
GCTGCAGTGCCCGCTGTTGT+CGG 0.418316 4:+40741834 None:intergenic
ATTTGCTGCGGGCGAAACTC+TGG 0.420271 4:+40744099 None:intergenic
AGTTAGAAGGTTCTAGGCTT+CGG 0.429264 4:-40741874 MsaT019908.1:CDS
TTGAAAGGAAACTACCTTGA+TGG 0.429916 4:+40739826 None:intergenic
AACACTACTCCTTCTCATTC+AGG 0.436567 4:-40739468 MsaT019908.1:CDS
AATGGTGTTTCTCTAACTCT+TGG 0.436577 4:-40739612 MsaT019908.1:CDS
TTGATATTGAAAACAGAAAG+AGG 0.441083 4:-40739428 MsaT019908.1:CDS
ACGCCTTACCGGTCGGCCTC+TGG 0.447088 4:-40744018 MsaT019908.1:CDS
TGGTGAGATTGAGGTATCTC+CGG 0.447805 4:-40743998 MsaT019908.1:CDS
GTTATTCTCCTCTTGCTCCT+TGG 0.449308 4:+40740088 None:intergenic
AGGAGAACCATTATTGTTAG+TGG 0.450941 4:+40744410 None:intergenic
TCATCATTTGAGCAAGCAAC+AGG 0.451763 4:-40742034 MsaT019908.1:CDS
CTCAATCAACACCACTTGCT+TGG 0.452628 4:-40744345 MsaT019908.1:CDS
TTGAGATTGTCTTCAAGGCT+AGG 0.454466 4:+40741984 None:intergenic
GCTCCTAGGTTCCATGGAAA+TGG 0.455886 4:-40739630 MsaT019908.1:CDS
TTGAAATCACTGTTGGAGAA+AGG 0.457475 4:+40739964 None:intergenic
AAGACAGCTAGCAAGACTTT+GGG 0.459364 4:-40741920 MsaT019908.1:CDS
TTGGGTGAAGATGATTGCTT+GGG 0.462431 4:-40741902 MsaT019908.1:CDS
TTGGAACTTGAAATCACTGT+TGG 0.463404 4:+40739957 None:intergenic
CAGCGGGCACTGCAGCAACT+TGG 0.465763 4:-40741827 MsaT019908.1:CDS
TTAGTGAGCAATTAGCTCCT+AGG 0.468409 4:-40739644 MsaT019908.1:CDS
GTACGTAGTTTGGATTCATA+AGG 0.468791 4:+40744493 None:intergenic
TGAAGTTGTGAATGTGGATA+AGG 0.475584 4:-40743881 MsaT019908.1:CDS
TCTCAGAATTTACATTTAGG+AGG 0.475857 4:-40739537 MsaT019908.1:CDS
CCACTATAACATGTGGGGAT+CGG 0.478000 4:-40744205 MsaT019908.1:CDS
GGTAGCCGTGGAGGCGAGGA+AGG 0.483983 4:-40743976 MsaT019908.1:CDS
CCACCAACTCCGACAACAGC+GGG 0.492092 4:-40741843 MsaT019908.1:CDS
CTGAGAGAGAAATCCATGTT+GGG 0.492621 4:+40739554 None:intergenic
GTGCCCGCTGTTGTCGGAGT+TGG 0.494381 4:+40741840 None:intergenic
GAATGATTCATAGCAATGCT+TGG 0.495045 4:-40741805 MsaT019908.1:CDS
AACTGGACTTACTAGGAGTC+AGG 0.495725 4:-40740920 MsaT019908.1:intron
CAATTAGCTCCTAGGTTCCA+TGG 0.502313 4:-40739636 MsaT019908.1:CDS
TCACCAGAGGCCGACCGGTA+AGG 0.503130 4:+40744015 None:intergenic
AAGTACATTTCTTCAACCAT+TGG 0.504400 4:+40740119 None:intergenic
AATGAATACCCTTTATTGAG+TGG 0.507581 4:-40739732 MsaT019908.1:CDS
TGAGATTGTCTTCAAGGCTA+GGG 0.509850 4:+40741985 None:intergenic
GAAGTTGTGAATGTGGATAA+GGG 0.514101 4:-40743880 MsaT019908.1:CDS
TGGTTTCCACAAGCGAACTC+TGG 0.515046 4:+40740139 None:intergenic
TAGTTTCCTTTCAATGGACA+TGG 0.518524 4:-40739817 MsaT019908.1:CDS
TCAATTCCATGTCCATTGAA+AGG 0.523266 4:+40739811 None:intergenic
CTCAGAATTTACATTTAGGA+GGG 0.524512 4:-40739536 MsaT019908.1:CDS
TTCAATGTCAACATCACCAA+TGG 0.525243 4:-40739931 MsaT019908.1:CDS
CCTCTCTCTTCAACCCAACA+TGG 0.526082 4:-40739567 MsaT019908.1:CDS
TGTAGGAGTGTCGGTAGTGT+TGG 0.526282 4:+40744290 None:intergenic
GAAACCAGAATTGGATTGAA+AGG 0.527024 4:+40739893 None:intergenic
CCCGCTGTTGTCGGAGTTGG+TGG 0.528625 4:+40741843 None:intergenic
GGTGAGATTGAGGTATCTCC+GGG 0.529905 4:-40743997 MsaT019908.1:CDS
TTATTGTTAGTGGTAGCGTT+GGG 0.532546 4:+40744420 None:intergenic
TCCGGGTAGCCGTGGAGGCG+AGG 0.533224 4:-40743980 MsaT019908.1:CDS
TTTGGGTGAAGATGATTGCT+TGG 0.533846 4:-40741903 MsaT019908.1:CDS
GACCCTCCTTCTCCTCCCAT+TGG 0.534808 4:+40739915 None:intergenic
AGTGTTGGATCCATGAAAGT+TGG 0.535269 4:+40744305 None:intergenic
TCTGAGAGAGAAATCCATGT+TGG 0.535848 4:+40739553 None:intergenic
GAAACTTCATATGGTTGTGT+AGG 0.538056 4:+40744273 None:intergenic
TAAACGCCAGAGTTCGCTTG+TGG 0.538237 4:-40740145 MsaT019908.1:CDS
AATGGTTGAAGAAATGTACT+TGG 0.541831 4:-40740117 MsaT019908.1:CDS
TGAAAGGAAACTACCTTGAT+GGG 0.542083 4:+40739827 None:intergenic
GTCGGCCTCTGGTGAGATTG+AGG 0.544425 4:-40744007 MsaT019908.1:CDS
TTATCCCAAGGACTCCGATA+AGG 0.544643 4:-40740959 MsaT019908.1:CDS
CATCAGATGATCCTGCAAGA+TGG 0.545078 4:+40739871 None:intergenic
CGATCCCCACATGTTATAGT+GGG 0.547541 4:+40744206 None:intergenic
CGTGGAGGCGAGGAAGGTGG+TGG 0.547728 4:-40743970 MsaT019908.1:CDS
ATAACCTTATCGGAGTCCTT+GGG 0.549058 4:+40740955 None:intergenic
ATGAGAAGGAGTAGTGTTGA+TGG 0.552006 4:+40739473 None:intergenic
CAATCTCACCAGAGGCCGAC+CGG 0.552708 4:+40744010 None:intergenic
TCCTCGCCTCCACGGCTACC+CGG 0.553350 4:+40743979 None:intergenic
ATTATTGTTAGTGGTAGCGT+TGG 0.555283 4:+40744419 None:intergenic
ACATCACCAATGGGAGGAGA+AGG 0.558319 4:-40739921 MsaT019908.1:CDS
TTAGAGAAACACCATTTCCA+TGG 0.559032 4:+40739619 None:intergenic
TCAATGTCAACATCACCAAT+GGG 0.559905 4:-40739930 MsaT019908.1:CDS
GTAAACCTTCTACCGACGGT+TGG 0.560766 4:+40744526 None:intergenic
TATGAAAACCAATTTGCTGC+GGG 0.560781 4:+40744088 None:intergenic
TAATTTGTTCCTGAATGAGA+AGG 0.560909 4:+40739459 None:intergenic
TTCAACTTCAAACTCTGTGG+TGG 0.562434 4:+40739773 None:intergenic
CTCGTCCCCACTATAACATG+TGG 0.566142 4:-40744212 MsaT019908.1:CDS
AGCCGTGGAGGCGAGGAAGG+TGG 0.567527 4:-40743973 MsaT019908.1:CDS
GGAAGAAACCAAGGAGCAAG+AGG 0.569522 4:-40740096 MsaT019908.1:CDS
TCACCAATGGGAGGAGAAGG+AGG 0.571505 4:-40739918 MsaT019908.1:CDS
GAACAATGTAGGTAGAGCTA+AGG 0.571744 4:-40742093 MsaT019908.1:intron
ACGAGGAGTCACGGTAGCCG+AGG 0.573764 4:+40744230 None:intergenic
TGGTTATGGATCATTCTCAA+TGG 0.575128 4:-40739688 MsaT019908.1:CDS
AGTTCGCTTGTGGAAACCAA+TGG 0.575659 4:-40740135 MsaT019908.1:CDS
GTAATAACAACAAAGAGAAG+AGG 0.583452 4:-40743822 MsaT019908.1:CDS
AATATTGAATCTAGTTCAAG+TGG 0.585962 4:-40743793 MsaT019908.1:CDS
TGATTCATAGCAATGCTTGG+AGG 0.588232 4:-40741802 MsaT019908.1:CDS
TGGTGTTGATTGAGTGATGA+AGG 0.588479 4:+40744354 None:intergenic
GTGGATAAGGGAATCATCAA+AGG 0.589254 4:-40743868 MsaT019908.1:CDS
CACCACCTTCCTCGCCTCCA+CGG 0.590288 4:+40743971 None:intergenic
CACATGTTATAGTGGGGACG+AGG 0.591269 4:+40744213 None:intergenic
AATCATCAAAGGAGAATCTG+TGG 0.597233 4:-40743857 MsaT019908.1:CDS
CGAGGAGGAAAATGGATGGA+AGG 0.598355 4:+40744248 None:intergenic
TCACACTTTGCATTTGATGG+TGG 0.598415 4:+40742059 None:intergenic
TGGAAGGTGGAAACTTCATA+TGG 0.602748 4:+40744264 None:intergenic
ATATGGTTGTGTAGGAGTGT+CGG 0.603447 4:+40744281 None:intergenic
GAGGTATCTCCGGGTAGCCG+TGG 0.603840 4:-40743988 MsaT019908.1:CDS
ACACCATTTCCATGGAACCT+AGG 0.604931 4:+40739627 None:intergenic
CAGGATCATCTGATGTGCAA+AGG 0.605084 4:-40739863 MsaT019908.1:CDS
GGTGGAGTTTCGACCATGAA+CGG 0.607341 4:-40743952 MsaT019908.1:CDS
GTCTGTAAACCTTCTACCGA+CGG 0.607577 4:+40744522 None:intergenic
ATCAAAGGAGAATCTGTGGA+AGG 0.608300 4:-40743853 MsaT019908.1:CDS
CCATGTTGGGTTGAAGAGAG+AGG 0.608631 4:+40739567 None:intergenic
TCGTCCCCACTATAACATGT+GGG 0.609880 4:-40744211 MsaT019908.1:CDS
CTTTCAGGCAAGCCTCTCTG+AGG 0.615305 4:+40741779 None:intergenic
GGTGGAAGAGAAAACAATGG+AGG 0.618595 4:-40743772 MsaT019908.1:CDS
CTATGAAAACCAATTTGCTG+CGG 0.619359 4:+40744087 None:intergenic
TCTTGATGAAGTTGTGAATG+TGG 0.620303 4:-40743887 MsaT019908.1:CDS
ATTGAATCTAGTTCAAGTGG+TGG 0.621430 4:-40743790 MsaT019908.1:CDS
GGAGGAAAATGGATGGAAGG+TGG 0.623156 4:+40744251 None:intergenic
AATGAAAATCATCATGGTGG+TGG 0.625687 4:-40739708 MsaT019908.1:CDS
AATGTACTTGGAAGAAACCA+AGG 0.627170 4:-40740105 MsaT019908.1:CDS
AGTGGAAATGAAAATCATCA+TGG 0.627811 4:-40739714 MsaT019908.1:CDS
AGGAGTCACGGTAGCCGAGG+AGG 0.628325 4:+40744233 None:intergenic
GATCCATGAAAGTTGGAAGG+TGG 0.629051 4:+40744312 None:intergenic
TAGCCGAGGAGGAAAATGGA+TGG 0.630526 4:+40744244 None:intergenic
TTAAATCCATGAAGCGCGTG+AGG 0.631215 4:+40744390 None:intergenic
GCTTGTTAGCATGCTAGATG+AGG 0.632117 4:-40743689 MsaT019908.1:intron
GTATCTCCGGGTAGCCGTGG+AGG 0.632306 4:-40743985 MsaT019908.1:CDS
AGAGGAGAATAACGACTCCA+AGG 0.633442 4:-40740078 MsaT019908.1:CDS
GTGGAAGAGAAAACAATGGA+GGG 0.633833 4:-40743771 MsaT019908.1:CDS
ATTGAGTGATGAAGGCGCGT+GGG 0.637713 4:+40744362 None:intergenic
ATAGTGGGGACGAGGAGTCA+CGG 0.640127 4:+40744221 None:intergenic
CATAGACCAGTTTATCAGCG+TGG 0.640750 4:-40744069 MsaT019908.1:CDS
TCTTTCAACTTCAAACTCTG+TGG 0.642321 4:+40739770 None:intergenic
TGTGACCTGCAGTTATCCCA+AGG 0.642673 4:-40740971 MsaT019908.1:intron
CACCAATGGGAGGAGAAGGA+GGG 0.642966 4:-40739917 MsaT019908.1:CDS
GAATAGTAACTCCAAGCAAG+TGG 0.643953 4:+40744334 None:intergenic
AAAGGAGAATCTGTGGAAGG+AGG 0.648166 4:-40743850 MsaT019908.1:CDS
GATTGAGTGATGAAGGCGCG+TGG 0.659202 4:+40744361 None:intergenic
CGTCCCCACTATAACATGTG+GGG 0.666378 4:-40744210 MsaT019908.1:CDS
CAGTTCTGAAATCCAACCGT+CGG 0.674796 4:-40744538 MsaT019908.1:CDS
ACCACCAACTCCGACAACAG+CGG 0.680001 4:-40741844 MsaT019908.1:CDS
TTGGATCCATGAAAGTTGGA+AGG 0.681916 4:+40744309 None:intergenic
AGATACCTCAATCTCACCAG+AGG 0.686383 4:+40744002 None:intergenic
GCAACAAACGCCTTACCGGT+CGG 0.687220 4:-40744025 MsaT019908.1:CDS
TTGAGTGATGAAGGCGCGTG+GGG 0.689955 4:+40744363 None:intergenic
ATGTCAACATCACCAATGGG+AGG 0.690189 4:-40739927 MsaT019908.1:CDS
AGTGGTGGAAGAGAAAACAA+TGG 0.709372 4:-40743775 MsaT019908.1:CDS
AAACTACGTACCTTACTCTG+AGG 0.712519 4:-40744481 MsaT019908.1:CDS
GATCCCCACATGTTATAGTG+GGG 0.721803 4:+40744207 None:intergenic
AATGCTTGGAGGCCTCAGAG+AGG 0.737120 4:-40741791 MsaT019908.1:CDS
GGAAATGAAAATCATCATGG+TGG 0.766816 4:-40739711 MsaT019908.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 40739392 40744595 40739392 ID=MsaG019908
Chr4 mRNA 40739392 40744595 40739392 ID=MsaT019908.1;Parent=MsaG019908
Chr4 exon 40739392 40740183 40739392 ID=MsaT019908.1.exon4;Parent=MsaT019908.1
Chr4 CDS 40739392 40740183 40739392 ID=cds.MsaT019908.1;Parent=MsaT019908.1
Chr4 exon 40740921 40740981 40740921 ID=MsaT019908.1.exon3;Parent=MsaT019908.1
Chr4 CDS 40740921 40740981 40740921 ID=cds.MsaT019908.1;Parent=MsaT019908.1
Chr4 exon 40741731 40742104 40741731 ID=MsaT019908.1.exon2;Parent=MsaT019908.1
Chr4 CDS 40741731 40742104 40741731 ID=cds.MsaT019908.1;Parent=MsaT019908.1
Chr4 exon 40743690 40744595 40743690 ID=MsaT019908.1.exon1;Parent=MsaT019908.1
Chr4 CDS 40743690 40744595 40743690 ID=cds.MsaT019908.1;Parent=MsaT019908.1
Gene Sequence

>MsaT019908.1

ATGGCAACGTACTTTCATGGCAACAGCAACAGCAACAGTTCTGAAATCCAACCGTCGGTAGAAGGTTTACAGACACTTTACCTTATGAATCCAAACTACGTACCTTACTCTGAGGAACCACAAAATCCAACACAAAACATGCTTTTCGCTAATCCCAACGCTACCACTAACAATAATGGTTCTCCTCACGCGCTTCATGGATTTAAGTTTCCCCACGCGCCTTCATCACTCAATCAACACCACTTGCTTGGAGTTACTATTCCACCTTCCAACTTTCATGGATCCAACACTACCGACACTCCTACACAACCATATGAAGTTTCCACCTTCCATCCATTTTCCTCCTCGGCTACCGTGACTCCTCGTCCCCACTATAACATGTGGGGATCGGGTAGCTTTCTACCTGATAACGCTGTCACAGCAACGACAACTGATAGATCAGAATGTTCAGATGTCGTTTCTGTGACAGCATCTCCAGAGTTTCGCCCGCAGCAAATTGGTTTTCATAGACCAGTTTATCAGCGTGGTTTGTCTCTCAGTCTTTCTTCGCAACAAACGCCTTACCGGTCGGCCTCTGGTGAGATTGAGGTATCTCCGGGTAGCCGTGGAGGCGAGGAAGGTGGTGGAGTTTCGACCATGAACGGTGTTATTTTCGGCTCGAAGTATATGAAAGTAGCACAAGAGCTTCTTGATGAAGTTGTGAATGTGGATAAGGGAATCATCAAAGGAGAATCTGTGGAAGGAGGTAATAGTAATAACAACAAAGAGAAGAGGAAAGCAAATATTGAATCTAGTTCAAGTGGTGGAAGAGAAAACAATGGAGGGAAACAAGTAGCTGAATTAAGCACTGCACAAAGACAAGAACTTCAGATGAAGAAGTCAAAGCTTGTTAGCATGCTAGATGAGGTAGAGCTAAGGTACAGACAATACCACCATCAAATGCAAAGTGTGATATCATCATTTGAGCAAGCAACAGGATATGGAGCATCAAAATCTTACACATCCCTAGCCTTGAAGACAATCTCAAAGCAGTTTCGAAGTCTAAAAGATTCAATCTCTTCACAAATTAAGACAGCTAGCAAGACTTTGGGTGAAGATGATTGCTTGGGTGTTAAGTTAGAAGGTTCTAGGCTTCGGTATGTCGACCACCAACTCCGACAACAGCGGGCACTGCAGCAACTTGGAATGATTCATAGCAATGCTTGGAGGCCTCAGAGAGGCTTGCCTGAAAGAGCTGTTTCTGTTCTTCGTGCTTGGCTTTTTGAACATTTCTTGCACCCTTATCCCAAGGACTCCGATAAGGTTATGCTTGCAAGACAAACTGGACTTACTAGGAGTCAGGTATCTAACTGGTTTATAAACGCCAGAGTTCGCTTGTGGAAACCAATGGTTGAAGAAATGTACTTGGAAGAAACCAAGGAGCAAGAGGAGAATAACGACTCCAAGGATAACACAAATAGATCAAAAGAATTAGGTGCCGCAAAACTTGATCAAATTAATAATCTTCATTCAAAAGCAGAAAACTTCAACAACCAAAACCTTTCTCCAACAGTGATTTCAAGTTCCAATAATTCAATGTCAACATCACCAATGGGAGGAGAAGGAGGGTCCTTTCAATCCAATTCTGGTTTCCATCTTGCAGGATCATCTGATGTGCAAAGGAGTCCAAAAAACTCCCATCAAGGTAGTTTCCTTTCAATGGACATGGAATTGAAGTCTAATAGAGAGTCCACCACAGAGTTTGAAGTTGAAAGACATAATCATGACAAAAATGAATACCCTTTATTGAGTGGAAATGAAAATCATCATGGTGGTGGTTATGGATCATTCTCAATGGAAGATGTTAGAAGGTTTAATGTTAGTGAGCAATTAGCTCCTAGGTTCCATGGAAATGGTGTTTCTCTAACTCTTGGACTACCTCATAATGAAAATATTCCTCTCTCTTCAACCCAACATGGATTTCTCTCTCAGAATTTACATTTAGGAGGGACGATTGATGAAATGGGAAGCAATGAGAATGAGTTTTGTACCATCAACACTACTCCTTCTCATTCAGGAACAAATTATGAGAGTATTGATATTGAAAACAGAAAGAGGTTTGCTGCTCAATTATTGCGAAATTTTGTGACCTAG
Protein sequence

>MsaT019908.1

MATYFHGNSNSNSSEIQPSVEGLQTLYLMNPNYVPYSEEPQNPTQNMLFANPNATTNNNGSPHALHGFKFPHAPSSLNQHHLLGVTIPPSNFHGSNTTDTPTQPYEVSTFHPFSSSATVTPRPHYNMWGSGSFLPDNAVTATTTDRSECSDVVSVTASPEFRPQQIGFHRPVYQRGLSLSLSSQQTPYRSASGEIEVSPGSRGGEEGGGVSTMNGVIFGSKYMKVAQELLDEVVNVDKGIIKGESVEGGNSNNNKEKRKANIESSSSGGRENNGGKQVAELSTAQRQELQMKKSKLVSMLDEVELRYRQYHHQMQSVISSFEQATGYGASKSYTSLALKTISKQFRSLKDSISSQIKTASKTLGEDDCLGVKLEGSRLRYVDHQLRQQRALQQLGMIHSNAWRPQRGLPERAVSVLRAWLFEHFLHPYPKDSDKVMLARQTGLTRSQVSNWFINARVRLWKPMVEEMYLEETKEQEENNDSKDNTNRSKELGAAKLDQINNLHSKAENFNNQNLSPTVISSSNNSMSTSPMGGEGGSFQSNSGFHLAGSSDVQRSPKNSHQGSFLSMDMELKSNRESTTEFEVERHNHDKNEYPLLSGNENHHGGGYGSFSMEDVRRFNVSEQLAPRFHGNGVSLTLGLPHNENIPLSSTQHGFLSQNLHLGGTIDEMGSNENEFCTINTTPSHSGTNYESIDIENRKRFAAQLLRNFVT*