AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG019971

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019971 MtrunA17_Chr4g0025061 77.698 556 65 5 1 502 1 551 0.0 857
MsaG019971 MtrunA17_Chr4g0025051 67.698 517 106 6 32 493 7 517 0.0 666
MsaG019971 MtrunA17_Chr4g0024391 68.150 427 100 5 108 504 1 421 0.0 566
MsaG019971 MtrunA17_Chr2g0322391 41.573 445 189 15 79 502 142 536 6.62e-89 284
MsaG019971 MtrunA17_Chr4g0075201 39.110 427 172 14 79 489 91 445 3.54e-70 232
MsaG019971 MtrunA17_Chr2g0288871 36.591 440 167 13 82 484 118 482 4.93e-58 201
MsaG019971 MtrunA17_Chr3g0099641 34.783 368 170 10 107 459 93 405 3.20e-56 194
MsaG019971 MtrunA17_Chr2g0302391 46.569 204 85 7 305 487 196 396 3.75e-42 155
MsaG019971 MtrunA17_Chr7g0270831 56.757 148 44 4 305 437 201 343 8.82e-42 154
MsaG019971 MtrunA17_Chr7g0216891 32.673 303 150 8 98 386 31 293 1.45e-34 137
MsaG019971 MtrunA17_Chr4g0024401 58.120 117 24 2 16 110 8 121 2.39e-32 120
MsaG019971 MtrunA17_Chr4g0051181 59.406 101 33 1 305 397 228 328 5.65e-30 122
MsaG019971 MtrunA17_Chr4g0060271 43.455 191 68 5 281 431 198 388 3.93e-28 118
MsaG019971 MtrunA17_Chr5g0419241 67.925 53 17 0 298 350 55 107 3.92e-22 91.3
MsaG019971 MtrunA17_Chr3g0101131 41.284 109 52 3 293 390 388 495 1.73e-20 94.7
MsaG019971 MtrunA17_Chr5g0418191 47.423 97 38 3 290 374 286 381 2.48e-20 93.6
MsaG019971 MtrunA17_Chr5g0418191 36.364 110 58 4 321 422 152 257 5.80e-11 64.7
MsaG019971 MtrunA17_Chr7g0218081 45.361 97 44 2 296 384 386 481 3.94e-20 93.6
MsaG019971 MtrunA17_Chr5g0432161 46.809 94 39 3 290 374 391 482 1.16e-19 92.4
MsaG019971 MtrunA17_Chr4g0001361 32.667 150 70 4 297 438 139 265 2.74e-19 89.0
MsaG019971 MtrunA17_Chr3g0079691 50.617 81 31 2 302 374 477 556 7.44e-19 90.1
MsaG019971 MtrunA17_Chr4g0042831 48.148 81 33 2 302 374 260 339 7.94e-19 88.6
MsaG019971 MtrunA17_Chr3g0106491 43.158 95 45 2 287 373 332 425 9.98e-19 89.0
MsaG019971 MtrunA17_Chr7g0249301 51.316 76 28 2 307 374 279 353 1.87e-18 87.0
MsaG019971 MtrunA17_Chr4g0004911 49.367 79 31 2 302 372 318 395 3.05e-18 87.4
MsaG019971 MtrunA17_Chr5g0445311 33.333 168 70 5 303 459 347 483 3.14e-18 87.8
MsaG019971 MtrunA17_Chr5g0447701 50.602 83 31 3 301 374 232 313 4.96e-18 85.9
MsaG019971 MtrunA17_Chr7g0249311 48.148 81 33 2 302 374 374 453 5.43e-18 87.4
MsaG019971 MtrunA17_Chr7g0243891 49.383 81 32 2 302 374 356 435 6.88e-18 86.7
MsaG019971 MtrunA17_Chr3g0136961 37.755 98 52 2 281 369 98 195 3.51e-17 82.0
MsaG019971 MtrunA17_Chr7g0270191 42.553 94 41 3 305 390 130 218 4.94e-17 81.6
MsaG019971 MtrunA17_Chr2g0333851 44.872 78 33 2 306 373 153 230 5.26e-17 82.0
MsaG019971 MtrunA17_Chr1g0150281 42.391 92 45 1 305 388 125 216 8.77e-17 80.9
MsaG019971 MtrunA17_Chr4g0051881 37.963 108 58 2 307 406 165 271 1.11e-16 81.3
MsaG019971 MtrunA17_Chr1g0150801 50.000 74 28 2 305 370 87 159 1.40e-16 77.4
MsaG019971 MtrunA17_Chr7g0263531 39.796 98 40 3 283 372 334 420 3.40e-16 80.9
MsaG019971 MtrunA17_Chr1g0150271 48.485 66 26 1 315 372 96 161 4.11e-16 78.2
MsaG019971 MtrunA17_Chr4g0074001 35.032 157 75 5 245 376 268 422 1.00e-15 79.7
MsaG019971 MtrunA17_Chr2g0293481 30.636 173 92 6 237 382 166 337 1.44e-15 79.3
MsaG019971 MtrunA17_Chr2g0294231 46.154 78 33 2 307 376 190 266 1.52e-15 78.6
MsaG019971 MtrunA17_Chr4g0053281 53.226 62 21 1 321 374 267 328 2.02e-15 77.4
MsaG019971 MtrunA17_Chr1g0194481 42.222 90 39 3 307 388 145 229 3.81e-15 76.6
MsaG019971 MtrunA17_Chr1g0147881 50.746 67 25 1 321 379 263 329 6.47e-15 75.9
MsaG019971 MtrunA17_Chr5g0401821 53.333 60 20 1 320 371 199 258 9.84e-15 74.3
MsaG019971 MtrunA17_Chr8g0386241 32.110 109 65 2 278 378 61 168 1.66e-14 72.0
MsaG019971 MtrunA17_Chr5g0443131 36.508 126 69 3 307 422 144 268 2.24e-14 73.9
MsaG019971 MtrunA17_Chr7g0224801 45.714 70 29 2 310 371 83 151 4.04e-14 70.5
MsaG019971 MtrunA17_Chr8g0379461 49.206 63 24 1 320 374 76 138 5.65e-14 71.6
MsaG019971 MtrunA17_Chr7g0270051 39.560 91 44 2 316 398 49 136 7.36e-14 71.6
MsaG019971 MtrunA17_Chr8g0335811 44.304 79 35 2 299 369 133 210 7.81e-14 70.9
MsaG019971 MtrunA17_Chr7g0248691 44.000 75 34 1 316 382 150 224 1.82e-13 71.6
MsaG019971 MtrunA17_Chr2g0319961 43.038 79 37 1 311 381 71 149 2.04e-13 70.5
MsaG019971 MtrunA17_Chr8g0348311 44.000 75 34 1 316 382 151 225 2.32e-13 71.2
MsaG019971 MtrunA17_Chr2g0289181 34.043 141 72 4 315 435 9 148 2.83e-13 69.3
MsaG019971 MtrunA17_Chr1g0184691 52.632 57 19 1 321 369 294 350 4.57e-13 70.5
MsaG019971 MtrunA17_Chr8g0383251 32.353 136 77 6 251 375 39 170 5.23e-13 67.8
MsaG019971 MtrunA17_Chr8g0387501 41.667 72 34 1 319 382 128 199 6.21e-13 69.3
MsaG019971 MtrunA17_Chr4g0068541 43.421 76 34 2 306 373 194 268 8.17e-13 70.1
MsaG019971 MtrunA17_Chr4g0071521 50.000 58 21 1 320 369 245 302 8.25e-13 69.3
MsaG019971 MtrunA17_Chr2g0284321 45.902 61 25 1 320 372 35 95 8.85e-13 67.8
MsaG019971 MtrunA17_Chr3g0129361 39.130 92 46 3 307 390 131 220 9.02e-13 67.8
MsaG019971 MtrunA17_Chr1g0188941 45.455 66 27 2 315 372 46 110 1.23e-12 64.7
MsaG019971 MtrunA17_Chr1g0202461 38.144 97 50 2 314 402 255 349 1.63e-12 69.3
MsaG019971 MtrunA17_Chr5g0418781 43.590 78 35 2 300 369 104 180 2.60e-12 67.4
MsaG019971 MtrunA17_Chr7g0228941 43.478 69 30 2 309 369 42 109 4.88e-12 63.2
MsaG019971 MtrunA17_Chr3g0129321 42.667 75 34 2 307 373 105 178 2.35e-11 63.2
MsaG019971 MtrunA17_Chr8g0383351 34.211 114 64 4 279 383 118 229 3.36e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG019971 AT4G04450.1 40.220 455 202 13 59 490 65 472 4.59e-78 254
MsaG019971 AT4G04450.2 40.235 425 184 13 89 490 1 378 3.53e-71 233
MsaG019971 AT4G22070.4 38.876 445 194 13 79 491 83 481 1.84e-62 213
MsaG019971 AT4G22070.3 38.876 445 194 13 79 491 83 481 1.84e-62 213
MsaG019971 AT4G22070.2 38.876 445 194 13 79 491 83 481 1.84e-62 213
MsaG019971 AT4G22070.1 38.876 445 194 13 79 491 83 481 1.84e-62 213
MsaG019971 AT1G62300.1 38.228 429 177 14 80 480 130 498 3.98e-58 202
MsaG019971 AT4G01720.1 40.824 267 117 9 254 500 170 415 4.11e-34 134
MsaG019971 AT1G68150.1 58.763 97 32 1 305 393 220 316 1.36e-33 131
MsaG019971 AT1G69810.1 31.006 358 192 13 92 439 14 326 1.72e-33 131
MsaG019971 AT5G15130.2 49.315 146 62 3 300 433 165 310 5.66e-33 132
MsaG019971 AT5G15130.1 49.315 146 62 3 300 433 207 352 7.72e-33 132
MsaG019971 AT1G69810.2 43.407 182 83 6 268 439 97 268 1.32e-32 127
MsaG019971 AT1G18860.1 30.133 375 187 9 114 449 48 386 6.25e-30 123
MsaG019971 AT2G03340.1 41.379 116 50 3 267 374 371 476 8.28e-20 92.8
MsaG019971 AT2G38470.1 50.617 81 31 2 302 374 344 423 1.41e-19 92.0
MsaG019971 AT1G13960.2 50.633 79 30 2 302 372 238 315 2.18e-19 90.1
MsaG019971 AT1G13960.1 50.633 79 30 2 302 372 391 468 2.24e-19 91.7
MsaG019971 AT4G26640.5 48.750 80 32 2 303 374 188 266 2.37e-19 90.5
MsaG019971 AT4G26640.6 48.750 80 32 2 303 374 188 266 2.37e-19 90.5
MsaG019971 AT4G26640.4 48.750 80 32 2 303 374 188 266 2.37e-19 90.5
MsaG019971 AT4G26640.3 48.750 80 32 2 303 374 188 266 2.37e-19 90.5
MsaG019971 AT4G26640.7 48.750 80 32 2 303 374 324 402 4.36e-19 90.5
MsaG019971 AT4G26640.1 48.750 80 32 2 303 374 292 370 4.58e-19 90.5
MsaG019971 AT4G26640.2 48.750 80 32 2 303 374 364 442 5.21e-19 90.5
MsaG019971 AT4G31800.2 40.000 105 50 3 299 390 85 189 1.56e-18 85.5
MsaG019971 AT4G31800.1 40.000 105 50 3 299 390 150 254 2.07e-18 86.7
MsaG019971 AT5G07100.2 47.561 82 34 2 300 373 121 201 2.60e-18 84.3
MsaG019971 AT5G07100.4 47.561 82 34 2 300 373 121 201 2.60e-18 84.3
MsaG019971 AT5G07100.5 47.561 82 34 2 300 373 121 201 2.60e-18 84.3
MsaG019971 AT5G07100.3 47.561 82 34 2 300 373 121 201 2.60e-18 84.3
MsaG019971 AT5G56270.1 49.383 81 32 2 302 374 469 548 3.60e-18 88.2
MsaG019971 AT5G07100.1 45.882 85 37 2 300 376 214 297 5.50e-18 85.1
MsaG019971 AT2G04880.2 42.857 91 43 2 290 372 253 342 6.63e-18 86.7
MsaG019971 AT2G04880.1 42.857 91 43 2 290 372 277 366 7.37e-18 86.7
MsaG019971 AT5G26170.1 51.389 72 26 2 306 369 99 169 9.53e-18 81.3
MsaG019971 AT3G01080.2 45.349 86 38 2 295 372 319 403 1.02e-17 86.3
MsaG019971 AT2G25000.3 30.337 178 99 5 220 375 14 188 1.09e-17 83.2
MsaG019971 AT3G01080.1 45.349 86 38 2 295 372 281 365 1.18e-17 85.5
MsaG019971 AT1G29860.1 44.681 94 39 3 305 390 121 209 2.28e-17 82.8
MsaG019971 AT2G25000.2 31.579 152 90 4 235 375 18 166 6.14e-17 80.5
MsaG019971 AT2G25000.4 31.579 152 90 4 235 375 18 166 6.14e-17 80.5
MsaG019971 AT4G30935.1 39.200 125 64 4 288 402 299 421 6.77e-17 83.6
MsaG019971 AT2G25000.1 31.579 152 90 4 235 375 61 209 1.04e-16 80.9
MsaG019971 AT1G80840.1 42.308 78 35 2 306 373 130 207 1.05e-16 81.3
MsaG019971 AT4G18170.1 39.000 100 52 2 307 398 159 257 4.30e-16 79.7
MsaG019971 AT1G55600.1 46.053 76 32 2 307 374 294 368 9.06e-16 80.1
MsaG019971 AT1G64000.1 45.455 77 33 2 302 370 96 171 1.12e-15 75.9
MsaG019971 AT5G41570.1 39.394 99 47 3 307 397 85 178 1.37e-15 75.5
MsaG019971 AT5G46350.1 33.835 133 71 3 307 431 170 293 2.02e-15 77.8
MsaG019971 AT2G30250.1 41.860 86 41 2 299 376 307 391 2.19e-15 78.6
MsaG019971 AT5G49520.1 45.000 80 35 2 305 376 206 284 2.67e-15 78.2
MsaG019971 AT4G26440.1 47.368 76 31 2 307 374 359 433 5.13e-15 78.2
MsaG019971 AT2G23320.1 54.237 59 19 1 321 371 241 299 5.36e-15 76.3
MsaG019971 AT1G29280.1 45.714 70 30 1 311 372 65 134 7.37e-15 75.1
MsaG019971 AT4G24240.1 53.333 60 20 1 320 371 281 340 1.68e-14 75.5
MsaG019971 AT3G58710.2 45.205 73 32 1 311 375 61 133 2.90e-14 73.6
MsaG019971 AT3G58710.1 45.205 73 32 1 311 375 62 134 2.96e-14 73.6
MsaG019971 AT2G44745.1 36.607 112 58 4 269 369 92 201 3.83e-14 72.0
MsaG019971 AT2G30590.1 53.125 64 21 2 321 375 314 377 4.36e-14 74.3
MsaG019971 AT4G01250.1 42.857 84 38 2 307 382 117 198 4.60e-14 73.2
MsaG019971 AT4G23550.1 43.590 78 36 1 315 384 135 212 5.96e-14 73.2
MsaG019971 AT5G52830.1 44.156 77 35 1 314 382 159 235 8.24e-14 73.2
MsaG019971 AT2G34830.1 40.860 93 40 2 302 379 190 282 9.13e-14 73.6
MsaG019971 AT2G34830.2 40.860 93 40 2 302 379 199 291 1.01e-13 73.6
MsaG019971 AT5G64810.1 43.617 94 41 3 287 369 74 166 1.06e-13 70.1
MsaG019971 AT2G46130.1 44.048 84 37 3 296 370 5 87 1.12e-13 67.8
MsaG019971 AT2G47260.1 42.169 83 39 2 305 379 159 240 1.16e-13 72.8
MsaG019971 AT1G30650.1 40.860 93 40 2 302 379 192 284 1.19e-13 73.2
MsaG019971 AT5G13080.1 45.205 73 31 2 307 371 54 125 1.52e-13 68.6
MsaG019971 AT3G01970.1 44.444 72 31 2 306 369 51 121 2.03e-13 68.2
MsaG019971 AT3G62340.1 48.649 74 29 2 307 372 105 177 2.77e-13 70.9
MsaG019971 AT5G28650.1 56.604 53 15 1 325 369 267 319 3.32e-13 71.2
MsaG019971 AT3G04670.1 56.604 53 15 1 325 369 267 319 3.99e-13 70.9
MsaG019971 AT4G31800.3 49.231 65 28 1 299 358 150 214 4.14e-13 69.3
MsaG019971 AT4G31550.1 52.632 57 19 1 321 369 247 303 4.15e-13 70.9
MsaG019971 AT4G31550.2 52.632 57 19 1 321 369 246 302 4.47e-13 70.9
MsaG019971 AT2G24570.1 52.632 57 19 1 321 369 244 300 5.94e-13 70.5
MsaG019971 AT5G45050.4 28.395 162 98 4 248 397 971 1126 1.69e-12 70.9
MsaG019971 AT5G45050.1 30.882 136 80 3 270 397 1136 1265 1.78e-12 70.9
MsaG019971 AT5G45050.2 30.882 136 80 3 270 397 1108 1237 1.79e-12 70.9
MsaG019971 AT4G39410.1 46.479 71 29 2 307 369 210 279 4.22e-12 67.4
MsaG019971 AT1G69310.4 42.667 75 34 2 303 369 130 203 6.04e-12 67.0
MsaG019971 AT1G69310.2 42.667 75 34 2 303 369 130 203 6.04e-12 67.0
MsaG019971 AT1G69310.1 42.667 75 34 2 303 369 130 203 6.04e-12 67.0
MsaG019971 AT2G37260.3 49.231 65 24 2 315 371 264 327 1.21e-11 66.6
MsaG019971 AT2G37260.2 49.231 65 24 2 315 371 262 325 1.28e-11 66.6
MsaG019971 AT2G37260.1 49.231 65 24 2 315 371 344 407 1.56e-11 66.6
MsaG019971 AT2G21900.1 40.580 69 32 2 315 375 104 171 8.93e-11 62.0

Find 90 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAACTGATCTTGACTTCT+AGG 0.119265 4:-41906704 None:intergenic
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4:-41908034 None:intergenic
ACTTGTTTGGCGAGAATATT+TGG 0.186172 4:-41908166 None:intergenic
ATTGGAGCCAAGGAAATTTA+TGG 0.210224 4:+41906374 MsaT019971.1:CDS
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4:-41904970 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4:+41908034 MsaT019971.1:CDS
GGGAAAGAGTTGTTATTAAC+TGG 0.286280 4:-41904456 None:intergenic
GCGTTCCTTTGTGGAGTATT+TGG 0.301041 4:-41907995 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4:+41906294 MsaT019971.1:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4:+41906533 MsaT019971.1:CDS
CCAAGGAAATTTATGGAACT+TGG 0.328012 4:+41906381 MsaT019971.1:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4:+41906185 MsaT019971.1:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4:+41908138 MsaT019971.1:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4:-41907067 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4:+41906972 MsaT019971.1:CDS
GAATGGCGCATTAACTTGTT+TGG 0.360025 4:-41908179 None:intergenic
CTACTAGTATTGATAGTAAT+AGG 0.368547 4:-41904916 None:intergenic
CTGCAGCGATAATAAGCTTT+AGG 0.368605 4:-41907023 None:intergenic
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4:-41907803 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4:+41904981 MsaT019971.1:CDS
TCCCTTTCCTAGTCAATATA+AGG 0.384368 4:+41904547 MsaT019971.1:CDS
AACTGATTTGCGTTCCTTTG+TGG 0.385254 4:-41908004 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4:+41906162 MsaT019971.1:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4:+41906500 MsaT019971.1:CDS
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4:-41907797 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4:-41907959 None:intergenic
GAAATTTATGGAACTTGGAT+TGG 0.404712 4:+41906386 MsaT019971.1:CDS
ACTTGGATTGGCTACTGATA+AGG 0.406023 4:+41906398 MsaT019971.1:CDS
TAACATCCTCATTCTTACTT+AGG 0.406214 4:-41906769 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4:-41906293 None:intergenic
CTTGTTTCATGAGATGAGAT+TGG 0.411363 4:-41904594 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4:-41904477 None:intergenic
CCAAGTTCCATAAATTTCCT+TGG 0.419641 4:-41906381 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4:-41907796 None:intergenic
GTGTAATCAATTGTAATAGT+TGG 0.425606 4:-41907965 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4:-41907061 None:intergenic
AAAATGAGAATTAAGAATGA+TGG 0.434196 4:+41906467 MsaT019971.1:intron
AACTGAGAATGGTGGAGTAT+TGG 0.438418 4:+41906356 MsaT019971.1:CDS
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4:+41906523 MsaT019971.1:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4:+41907802 MsaT019971.1:CDS
GACCTTATATTGACTAGGAA+AGG 0.470626 4:-41904549 None:intergenic
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4:+41906704 MsaT019971.1:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4:+41906505 MsaT019971.1:CDS
GCATCAACATCAAGCAATGA+TGG 0.494138 4:+41907863 MsaT019971.1:CDS
GTTGGTGTCAAGGCAATGTA+AGG 0.496162 4:+41906613 MsaT019971.1:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4:+41906517 MsaT019971.1:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4:-41907052 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4:+41906483 MsaT019971.1:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4:-41907062 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4:+41906278 MsaT019971.1:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4:-41908145 None:intergenic
TCATGAAACAAGAACAGAGT+TGG 0.518840 4:+41904605 MsaT019971.1:CDS
TAGTAGTGATCAATCTATGA+TGG 0.520634 4:+41904933 MsaT019971.1:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4:-41907017 None:intergenic
ACCTTATATTGACTAGGAAA+GGG 0.537487 4:-41904548 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4:+41906951 MsaT019971.1:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4:-41904494 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.543545 4:+41907761 MsaT019971.1:intron
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4:-41908042 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4:+41906595 MsaT019971.1:CDS
AGTATTGATAGTAATAGGAA+TGG 0.556477 4:-41904911 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4:+41906551 MsaT019971.1:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.563407 4:-41907051 None:intergenic
GTATTGATAGTAATAGGAAT+GGG 0.563883 4:-41904910 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4:+41904984 MsaT019971.1:CDS
GATAATAAGCTTTAGGACAC+GGG 0.575823 4:-41907016 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4:+41904940 MsaT019971.1:CDS
ATGGTGGAGTATTGGAGCCA+AGG 0.577700 4:+41906364 MsaT019971.1:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4:+41906961 MsaT019971.1:intron
GTATTGCCAATATTTGTGAA+GGG 0.582929 4:-41908144 None:intergenic
GAATATTTGGACTAGCGGAA+TGG 0.586184 4:-41908196 None:intergenic
TATCGCTGCAGCATGACCCA+TGG 0.589148 4:+41907035 MsaT019971.1:CDS
TACAAATGCTGATATTGTGA+TGG 0.589604 4:+41906649 MsaT019971.1:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4:+41906763 MsaT019971.1:CDS
ATGGTGGAGAGTTGGTGTCA+AGG 0.595504 4:+41906603 MsaT019971.1:CDS
ATTAACTGGAACTGAAGCCA+TGG 0.605747 4:-41904442 None:intergenic
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4:-41908062 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4:+41906973 MsaT019971.1:CDS
TGGAGGACCTTATATTGACT+AGG 0.616940 4:-41904554 None:intergenic
TGTTAATGTTGATAATGTTG+AGG 0.619880 4:+41906787 MsaT019971.1:CDS
AAGTCAAGATCAGTTAGGAT+CGG 0.620379 4:+41906709 MsaT019971.1:CDS
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4:-41907793 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4:-41904476 None:intergenic
AGCTAGATCAGAAGAAACCA+TGG 0.628836 4:+41906841 MsaT019971.1:CDS
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4:-41908043 None:intergenic
TTCATGAGATGAGATTGGAG+AGG 0.650096 4:-41904589 None:intergenic
AGGCAGAGACAACTTCATGA+TGG 0.653739 4:-41904653 None:intergenic
AATCTCCAAATACTCCACAA+AGG 0.678231 4:+41907990 MsaT019971.1:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4:-41907923 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4:+41906279 MsaT019971.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 41904444 41908207 41904444 ID=MsaG019971
Chr4 mRNA 41904444 41908207 41904444 ID=MsaT019971.1;Parent=MsaG019971
Chr4 exon 41904444 41904662 41904444 ID=MsaT019971.1.exon1;Parent=MsaT019971.1
Chr4 CDS 41904444 41904662 41904444 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Chr4 exon 41904904 41905005 41904904 ID=MsaT019971.1.exon2;Parent=MsaT019971.1
Chr4 CDS 41904904 41905005 41904904 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Chr4 exon 41906168 41906419 41906168 ID=MsaT019971.1.exon3;Parent=MsaT019971.1
Chr4 CDS 41906168 41906419 41906168 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Chr4 exon 41906482 41906862 41906482 ID=MsaT019971.1.exon4;Parent=MsaT019971.1
Chr4 CDS 41906482 41906862 41906482 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Chr4 exon 41906963 41907080 41906963 ID=MsaT019971.1.exon5;Parent=MsaT019971.1
Chr4 CDS 41906963 41907080 41906963 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Chr4 exon 41907762 41908207 41907762 ID=MsaT019971.1.exon6;Parent=MsaT019971.1
Chr4 CDS 41907762 41908207 41907762 ID=cds.MsaT019971.1;Parent=MsaT019971.1
Gene Sequence

>MsaT019971.1

ATGGCTTCAGTTCCAGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATAAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCTCATGAAACAAGAACAGAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAACTTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCAGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTATTGGAGCCAAGGAAATTTATGGAACTTGGATTGGCTACTGATAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGTCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTCGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCGGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAAAGCTAGATCAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCATGACCCATGGTTGCCCAGTCCGAAAGCATGTAATTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGAAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCACACGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGCAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTCTCGCCAAACAAGTTAATGCGCCATTCCGCTAG
Protein sequence

>MsaT019971.1

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHSTIPFLVNIRSSNENQTSPISSHETRTELDFFDDNNNDHHELGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMQKRFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVLEPRKFMELGLATDKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELVSRQCKDLVLITNADIVMDHEASSSSMRKPRSQDQLGSAMKNIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIKARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSMTHGCPVRKHVIVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSASTSSNDGQLNENLLTRTLLPCSSSIATISHAPYPTITIDYTQSPNTPQRNANQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNILAKQVNAPFR*