Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020063 | XP_013442871.1 | 99.398 | 166 | 1 | 0 | 3 | 168 | 74 | 239 | 9.83e-118 | 342 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020063 | sp|Q8GZA8|ULT1_ARATH | 76.506 | 166 | 39 | 0 | 3 | 168 | 72 | 237 | 3.54e-94 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020063 | tr|A0A072TI70|A0A072TI70_MEDTR | 99.398 | 166 | 1 | 0 | 3 | 168 | 74 | 239 | 4.70e-118 | 342 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG020063 | TF | ULT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020063 | MtrunA17_Chr4g0022491 | 99.398 | 166 | 1 | 0 | 3 | 168 | 74 | 239 | 9.05e-122 | 342 |
| MsaG020063 | MtrunA17_Chr2g0323881 | 81.928 | 166 | 30 | 0 | 3 | 168 | 71 | 236 | 9.24e-101 | 289 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020063 | AT4G28190.2 | 76.190 | 168 | 40 | 0 | 1 | 168 | 101 | 268 | 2.54e-96 | 280 |
| MsaG020063 | AT4G28190.1 | 76.506 | 166 | 39 | 0 | 3 | 168 | 72 | 237 | 3.60e-95 | 275 |
| MsaG020063 | AT2G20825.1 | 69.048 | 168 | 46 | 1 | 1 | 168 | 66 | 227 | 3.11e-85 | 250 |
Find 33 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGACCAACCCGGCCATTT+TGG | 0.182520 | 4:+43784391 | None:intergenic |
| AATATCTGGGTTATAATTAA+TGG | 0.255756 | 4:-43784495 | MsaT020063.1:CDS |
| GGAAATGGAAGAATAATATC+TGG | 0.324881 | 4:-43784509 | MsaT020063.1:CDS |
| ATGAGTTTGTTCGCTGTACT+AGG | 0.392965 | 4:-43784365 | MsaT020063.1:CDS |
| ATGCTGTGTCAGATGCAAAT+TGG | 0.397171 | 4:-43784284 | MsaT020063.1:CDS |
| GGCTCTCAGAGATCCCAAAA+TGG | 0.420579 | 4:-43784405 | MsaT020063.1:CDS |
| GGTTATAATTAATGGTGATA+AGG | 0.431324 | 4:-43784487 | MsaT020063.1:CDS |
| GAAGAGAGACAGCAAGGAAA+TGG | 0.432232 | 4:-43784524 | MsaT020063.1:CDS |
| GAATAAACGTACTTCTCATA+TGG | 0.434948 | 4:+43784246 | None:intergenic |
| CAAGTGGCGAAAGCTGCTAA+TGG | 0.441867 | 4:-43784426 | MsaT020063.1:CDS |
| TGCACTTCTTGTGTGTGCTT+CGG | 0.456632 | 4:-43782601 | MsaT020063.1:CDS |
| TAATGGTGATAAGGTTCCAT+TGG | 0.460779 | 4:-43784478 | MsaT020063.1:CDS |
| CTCAGAGATCCCAAAATGGC+CGG | 0.484048 | 4:-43784401 | MsaT020063.1:CDS |
| AATGGTGATAAGGTTCCATT+GGG | 0.489767 | 4:-43784477 | MsaT020063.1:CDS |
| TGGTAAAGTCAGTACAAGTC+TGG | 0.500731 | 4:+43782545 | None:intergenic |
| AACCTTTGCATGTTGGTGAT+CGG | 0.518571 | 4:+43782623 | None:intergenic |
| ATTTCGTCTGAGATCTAAAG+AGG | 0.537276 | 4:-43784325 | MsaT020063.1:CDS |
| AGATCCCAAAATGGCCGGGT+TGG | 0.546651 | 4:-43784396 | MsaT020063.1:CDS |
| GAGTGTTTCTCAAATGCAGC+AGG | 0.552189 | 4:+43784549 | None:intergenic |
| TCAGAGATCCCAAAATGGCC+GGG | 0.567774 | 4:-43784400 | MsaT020063.1:CDS |
| GAAATGGAAGAATAATATCT+GGG | 0.580008 | 4:-43784508 | MsaT020063.1:CDS |
| GGCCGATCACCAACATGCAA+AGG | 0.580638 | 4:-43782625 | MsaT020063.1:CDS |
| CGAAGAGTTTACAGAGGATG+CGG | 0.588649 | 4:-43782646 | MsaT020063.1:CDS |
| GCTGCATTTGAGAAACACTC+TGG | 0.595645 | 4:-43784546 | MsaT020063.1:CDS |
| TTGCATCTGACACAGCATCA+TGG | 0.597955 | 4:+43784289 | None:intergenic |
| ACTCATCGCGGTGACCAACC+CGG | 0.606791 | 4:+43784382 | None:intergenic |
| GCTCAAATACTACAATCAAG+TGG | 0.629351 | 4:-43784442 | MsaT020063.1:CDS |
| ACACAGCATCATGGTGAATG+CGG | 0.634639 | 4:+43784298 | None:intergenic |
| GAAGTGCAACCTTTGCATGT+TGG | 0.651416 | 4:+43782616 | None:intergenic |
| GGAAGTCGAAGAGTTTACAG+AGG | 0.661639 | 4:-43782652 | MsaT020063.1:CDS |
| ACTCTGGAAGAGAGACAGCA+AGG | 0.669740 | 4:-43784530 | MsaT020063.1:CDS |
| TGTGATGATGAAGAAGAGCG+AGG | 0.697305 | 4:-43782673 | MsaT020063.1:CDS |
| TACAGCGAACAAACTCATCG+CGG | 0.760016 | 4:+43784370 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 43782528 | 43784589 | 43782528 | ID=MsaG020063 |
| Chr4 | mRNA | 43782528 | 43784589 | 43782528 | ID=MsaT020063.1;Parent=MsaG020063 |
| Chr4 | exon | 43782528 | 43782702 | 43782528 | ID=MsaT020063.1.exon2;Parent=MsaT020063.1 |
| Chr4 | CDS | 43782528 | 43782702 | 43782528 | ID=cds.MsaT020063.1;Parent=MsaT020063.1 |
| Chr4 | exon | 43784258 | 43784589 | 43784258 | ID=MsaT020063.1.exon1;Parent=MsaT020063.1 |
| Chr4 | CDS | 43784258 | 43784589 | 43784258 | ID=cds.MsaT020063.1;Parent=MsaT020063.1 |
| Gene Sequence |
| Protein sequence |
