Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020065 | XP_013455584.1 | 98.701 | 154 | 2 | 0 | 1 | 154 | 1 | 154 | 7.21e-109 | 316 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020065 | sp|O05517|RIMI_BACSU | 29.600 | 125 | 87 | 1 | 17 | 141 | 15 | 138 | 6.96e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020065 | tr|G7ZX36|G7ZX36_MEDTR | 98.701 | 154 | 2 | 0 | 1 | 154 | 1 | 154 | 3.45e-109 | 316 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG020065 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020065 | MtrunA17_Chr4g0022511 | 98.701 | 154 | 2 | 0 | 1 | 154 | 1 | 154 | 6.63e-113 | 316 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020065 | AT1G03650.1 | 69.079 | 152 | 44 | 1 | 6 | 154 | 6 | 157 | 6.73e-75 | 221 |
Find 29 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGGTTATGTCATGTATTCT+TGG | 0.193464 | 4:+43806137 | MsaT020065.1:CDS |
| AGTGGAAGAGATAGTGAAAT+TGG | 0.247431 | 4:+43806009 | MsaT020065.1:CDS |
| CACGTTGATGCCCAACTTGC+AGG | 0.261704 | 4:+43806118 | MsaT020065.1:CDS |
| CATGTTGACTGTTTGGTTGA+AGG | 0.355783 | 4:+43806943 | MsaT020065.1:CDS |
| GAAGCGAGTGATTCGTGTTT+GGG | 0.367842 | 4:-43806046 | None:intergenic |
| GGAAGCGAGTGATTCGTGTT+TGG | 0.373847 | 4:-43806047 | None:intergenic |
| TGGCTTTCATGTTGACTGTT+TGG | 0.383570 | 4:+43806936 | MsaT020065.1:CDS |
| CATGACATAACCTGCAAGTT+GGG | 0.434152 | 4:-43806128 | None:intergenic |
| TGGACTTCGATTCAAATTGA+AGG | 0.474763 | 4:+43807004 | MsaT020065.1:CDS |
| GCAGAACAAGAAAAGTTTCA+CGG | 0.515102 | 4:+43806857 | MsaT020065.1:CDS |
| AAATGCTTATAGAATGTACT+TGG | 0.517363 | 4:+43806984 | MsaT020065.1:CDS |
| CGCACGAGTGATGGCAAATG+TGG | 0.525694 | 4:+43805952 | None:intergenic |
| CAATGGAGGAGGCAAGGACA+CGG | 0.532439 | 4:+43806802 | MsaT020065.1:CDS |
| ACATGACATAACCTGCAAGT+TGG | 0.533996 | 4:-43806129 | None:intergenic |
| GGAGTTGGAACCTGCGAGCT+TGG | 0.538376 | 4:-43806185 | None:intergenic |
| GCTTCTATCACCAAGCTCGC+AGG | 0.549548 | 4:+43806175 | MsaT020065.1:CDS |
| AAGGAGCAATGGAGGAGGCA+AGG | 0.558770 | 4:+43806796 | MsaT020065.1:CDS |
| TGCTTCACGTCGATCCGTTG+AGG | 0.562820 | 4:+43806884 | MsaT020065.1:CDS |
| ATAGAAGCATACAATGAAGA+AGG | 0.562971 | 4:-43806160 | None:intergenic |
| CAGAAACAGTGGTAAAGTAG+TGG | 0.572015 | 4:+43805991 | MsaT020065.1:CDS |
| CTTCTGTAGTGAAGGAGCAA+TGG | 0.596774 | 4:+43806785 | MsaT020065.1:intron |
| CTGAATCTGTACAAGAAGCA+TGG | 0.599242 | 4:+43806916 | MsaT020065.1:CDS |
| CTGTAGTGAAGGAGCAATGG+AGG | 0.602271 | 4:+43806788 | MsaT020065.1:intron |
| TAGTGAAGGAGCAATGGAGG+AGG | 0.603189 | 4:+43806791 | MsaT020065.1:intron |
| CGGAGAGGTTCTACTGAAAG+CGG | 0.611656 | 4:+43806822 | MsaT020065.1:CDS |
| TTCAGCGCAGGTGTCCTCAA+CGG | 0.620997 | 4:-43806898 | None:intergenic |
| TTCTTGTACAGATTCAGCGC+AGG | 0.621123 | 4:-43806910 | None:intergenic |
| TTAGAACTTGACAGAAACAG+TGG | 0.638839 | 4:+43805980 | MsaT020065.1:CDS |
| GAGGAGGCAAGGACACGGAG+AGG | 0.683029 | 4:+43806807 | MsaT020065.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 43805962 | 43807023 | 43805962 | ID=MsaG020065 |
| Chr4 | mRNA | 43805962 | 43807023 | 43805962 | ID=MsaT020065.1;Parent=MsaG020065 |
| Chr4 | exon | 43805962 | 43806196 | 43805962 | ID=MsaT020065.1.exon1;Parent=MsaT020065.1 |
| Chr4 | CDS | 43805962 | 43806196 | 43805962 | ID=cds.MsaT020065.1;Parent=MsaT020065.1 |
| Chr4 | exon | 43806794 | 43807023 | 43806794 | ID=MsaT020065.1.exon2;Parent=MsaT020065.1 |
| Chr4 | CDS | 43806794 | 43807023 | 43806794 | ID=cds.MsaT020065.1;Parent=MsaT020065.1 |
| Gene Sequence |
| Protein sequence |
