Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020087 | KEH29599.1 | 83.333 | 408 | 47 | 4 | 1 | 401 | 1 | 394 | 0.0 | 665 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020087 | tr|A0A072UIK6|A0A072UIK6_MEDTR | 83.333 | 408 | 47 | 4 | 1 | 401 | 1 | 394 | 0.0 | 665 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG020087 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG007500 | MsaG020087 | 0.801020 | 1.835864e-48 | 2.539992e-46 |
| MsaG020087 | MsaG023813 | 0.808403 | 5.365026e-50 | 8.815573e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020087 | MtrunA17_Chr4g0022641 | 83.333 | 408 | 47 | 4 | 1 | 401 | 1 | 394 | 0.0 | 665 |
| MsaG020087 | MtrunA17_Chr4g0059631 | 45.317 | 331 | 103 | 4 | 1 | 329 | 1 | 255 | 3.29e-85 | 259 |
| MsaG020087 | MtrunA17_Chr1g0212471 | 54.106 | 207 | 74 | 4 | 1 | 205 | 1 | 188 | 1.21e-66 | 210 |
| MsaG020087 | MtrunA17_Chr2g0303981 | 48.837 | 172 | 85 | 3 | 3 | 172 | 5 | 175 | 5.50e-46 | 156 |
| MsaG020087 | MtrunA17_Chr6g0449851 | 36.139 | 202 | 114 | 4 | 1 | 198 | 1 | 191 | 8.58e-35 | 130 |
| MsaG020087 | MtrunA17_Chr6g0457851 | 33.161 | 193 | 126 | 1 | 1 | 190 | 11 | 203 | 1.83e-30 | 120 |
| MsaG020087 | MtrunA17_Chr1g0212151 | 34.975 | 203 | 124 | 3 | 1 | 198 | 1 | 200 | 8.08e-30 | 118 |
| MsaG020087 | MtrunA17_Chr6g0449861 | 35.294 | 170 | 107 | 1 | 1 | 167 | 9 | 178 | 1.81e-27 | 111 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020087 | AT5G55690.1 | 33.562 | 146 | 86 | 3 | 1 | 142 | 1 | 139 | 3.12e-15 | 75.9 |
| MsaG020087 | AT5G55690.2 | 33.562 | 146 | 86 | 3 | 1 | 142 | 1 | 139 | 3.12e-15 | 75.9 |
Find 53 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAGTCGACCTAATGATTT+TGG | 0.146545 | 4:-44065458 | MsaT020087.1:CDS |
| GCATTCAGATTTCCTTAATA+TGG | 0.236107 | 4:-44065996 | MsaT020087.1:CDS |
| TGAAGAGGGCAATGCAAATT+TGG | 0.258454 | 4:-44065198 | MsaT020087.1:CDS |
| TTGTGGACATCAAATATTTC+AGG | 0.290143 | 4:+44066103 | None:intergenic |
| CAATGCTCTCATGCAAAATA+TGG | 0.294005 | 4:-44065858 | MsaT020087.1:CDS |
| TCTAAATTTGTTAGCAAGAC+TGG | 0.334083 | 4:-44066253 | MsaT020087.1:CDS |
| TTTAACCAATCGTCAAAATC+AGG | 0.356658 | 4:+44065620 | None:intergenic |
| AAGTTGGATACTTCTTCTTA+AGG | 0.361888 | 4:+44066020 | None:intergenic |
| AGTTGGATACTTCTTCTTAA+GGG | 0.378758 | 4:+44066021 | None:intergenic |
| AACCAATAGCCAAAATCATT+AGG | 0.388302 | 4:+44065449 | None:intergenic |
| GGGTCTTCTGCCCATGTCAT+TGG | 0.398342 | 4:+44066181 | None:intergenic |
| TCTTAATTTCAAAGTATTTG+TGG | 0.402565 | 4:+44066086 | None:intergenic |
| TCTTTCCATGCAAACTGATC+AGG | 0.410075 | 4:+44065395 | None:intergenic |
| ATTAAAATTTATCTTTCCAT+TGG | 0.417218 | 4:+44065165 | None:intergenic |
| ATTTGGATGATTTGATCCAA+TGG | 0.435367 | 4:-44065181 | MsaT020087.1:CDS |
| AAGGGTATCTTTCCTCACTT+TGG | 0.441633 | 4:+44066039 | None:intergenic |
| AGCATGGAGTTTACAATTGT+AGG | 0.450184 | 4:+44066160 | None:intergenic |
| AGTTGCTTTGTTGACGAACT+AGG | 0.453824 | 4:+44065479 | None:intergenic |
| AGTTGCTTTGTTGATGAACT+AGG | 0.453927 | 4:+44065314 | None:intergenic |
| TCTCACTAATATCATGAAGT+TGG | 0.454769 | 4:+44065687 | None:intergenic |
| TGCTATGCATGACATTAGCT+TGG | 0.463751 | 4:+44065804 | None:intergenic |
| TGATGTATTGTTGCATTTGA+GGG | 0.469500 | 4:+44065774 | None:intergenic |
| ATGAGGCAAATCAACATGAT+TGG | 0.473560 | 4:+44065724 | None:intergenic |
| CAACAATACATCAATGGTAC+TGG | 0.473912 | 4:-44065764 | MsaT020087.1:CDS |
| GCATGGAGTTTACAATTGTA+GGG | 0.478326 | 4:+44066161 | None:intergenic |
| GATGGCAATGCAAGTTATCT+TGG | 0.483510 | 4:-44065347 | MsaT020087.1:CDS |
| TTGATGTATTGTTGCATTTG+AGG | 0.494116 | 4:+44065773 | None:intergenic |
| TTTGGCTATTGGTTAAATGA+TGG | 0.498816 | 4:-44065440 | MsaT020087.1:CDS |
| AGTTGCTTTGTAGATGAACT+AGG | 0.501324 | 4:+44065650 | None:intergenic |
| TGGTTTGTTGATGAATTATG+AGG | 0.510382 | 4:+44065707 | None:intergenic |
| AGGAAATCTGAATGCCAAGT+TGG | 0.511291 | 4:+44066004 | None:intergenic |
| GATTGGTTAAATCAACTAGA+TGG | 0.520594 | 4:-44065608 | MsaT020087.1:CDS |
| TTGATTGTGTATGATGATGA+TGG | 0.536425 | 4:-44066217 | MsaT020087.1:CDS |
| ATATGAGCATAAGAAGATTG+AGG | 0.538047 | 4:-44066131 | MsaT020087.1:CDS |
| CGGTTAAATCAACTAGATGA+TGG | 0.538310 | 4:-44065266 | MsaT020087.1:CDS |
| CAAATGCAACAATACATCAA+TGG | 0.539383 | 4:-44065770 | MsaT020087.1:CDS |
| GGTGATCAGTTTGCCTCGAA+AGG | 0.542852 | 4:-44065242 | MsaT020087.1:CDS |
| GATCGTCAAAATCATGAGGT+TGG | 0.544039 | 4:+44065288 | None:intergenic |
| TGATGATGATGGTAATTATG+TGG | 0.549083 | 4:-44066206 | MsaT020087.1:CDS |
| GTCAACCTGATCAGTTTGCA+TGG | 0.549898 | 4:-44065400 | MsaT020087.1:CDS |
| ATGCTCATATTCTTGAAGCA+TGG | 0.569236 | 4:+44066144 | None:intergenic |
| ATTATGTGGGACCAATGACA+TGG | 0.587619 | 4:-44066192 | MsaT020087.1:CDS |
| ATTAGCTTGGCTTGAATGTG+AGG | 0.604587 | 4:+44065817 | None:intergenic |
| CATCAATGGTACTGGTGAGA+TGG | 0.609901 | 4:-44065756 | MsaT020087.1:CDS |
| GATGATGATGGTAATTATGT+GGG | 0.610855 | 4:-44066205 | MsaT020087.1:CDS |
| AACCGATCGTCAAAATCATG+AGG | 0.617532 | 4:+44065284 | None:intergenic |
| CAATGGTACTGGTGAGATGG+TGG | 0.621142 | 4:-44065753 | MsaT020087.1:CDS |
| TGCTTTGTTGATGAACTAGG+AGG | 0.622470 | 4:+44065317 | None:intergenic |
| TTAAGAAGAAGTATCCAACT+TGG | 0.637255 | 4:-44066018 | MsaT020087.1:CDS |
| AAGCTAAGATAACCAAAGTG+AGG | 0.659175 | 4:-44066051 | MsaT020087.1:CDS |
| TTAAATCAACTAGATGATGG+TGG | 0.672684 | 4:-44065263 | MsaT020087.1:CDS |
| TGCTTTGTAGATGAACTAGG+AGG | 0.674710 | 4:+44065653 | None:intergenic |
| TTATGTGGGACCAATGACAT+GGG | 0.701198 | 4:-44066191 | MsaT020087.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 44065166 | 44066371 | 44065166 | ID=MsaG020087 |
| Chr4 | mRNA | 44065166 | 44066371 | 44065166 | ID=MsaT020087.1;Parent=MsaG020087 |
| Chr4 | exon | 44065166 | 44066371 | 44065166 | ID=MsaT020087.1.exon1;Parent=MsaT020087.1 |
| Chr4 | CDS | 44065166 | 44066371 | 44065166 | ID=cds.MsaT020087.1;Parent=MsaT020087.1 |
| Gene Sequence |
| Protein sequence |
