AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020185


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020185 MtrunA17_Chr2g0281511 79.508 122 14 1 1 111 1 122 2.84e-66 195
MsaG020185 MtrunA17_Chr2g0309121 73.770 122 20 2 1 111 1 121 5.87e-59 177
MsaG020185 MtrunA17_Chr3g0081891 77.500 80 7 1 1 69 1 80 1.91e-37 121
MsaG020185 MtrunA17_Chr7g0276661 42.857 77 43 1 33 109 92 167 2.35e-17 72.8
MsaG020185 MtrunA17_Chr5g0430431 33.696 92 57 1 18 105 163 254 1.23e-13 64.7
MsaG020185 MtrunA17_Chr8g0356961 31.313 99 55 2 24 109 77 175 2.16e-11 57.4
MsaG020185 MtrunA17_Chr0c01g0489371 35.165 91 46 2 22 99 224 314 7.69e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 20 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATGTTACTACTTCTATTT+TGG 0.146520 4:+46748554 MsaT020185.1:CDS
GAAGTCAATGGTACTGATTT+AGG 0.173353 4:+46748495 MsaT020185.1:CDS
CTTGAAAAGGGCCTAAAATT+TGG 0.185070 4:+46748817 MsaT020185.1:CDS
AAAATATCGATGTTCATTAT+TGG 0.232793 4:+46748526 MsaT020185.1:CDS
TACCAGTTTCTTCATCAAAA+AGG 0.252196 4:-46748714 None:intergenic
TGTATGTTACCGTCGTTAAC+TGG 0.292738 4:+46748881 MsaT020185.1:CDS
AAGTCAATGGTACTGATTTA+GGG 0.335811 4:+46748496 MsaT020185.1:CDS
CGGTCAATAATCCTCCTAAT+AGG 0.355854 4:+46748857 MsaT020185.1:CDS
TTTGGATGGTAACAACGTTT+TGG 0.356016 4:+46748572 MsaT020185.1:CDS
GTACGACTTTGTTCTTGAAA+AGG 0.412286 4:+46748804 MsaT020185.1:CDS
ACGGTAACATACATCCTATT+AGG 0.430681 4:-46748871 None:intergenic
TACGACTTTGTTCTTGAAAA+GGG 0.450393 4:+46748805 MsaT020185.1:CDS
GTATGTTACCGTCGTTAACT+GGG 0.530161 4:+46748882 MsaT020185.1:CDS
AGCATGCATCAGAAATACAT+AGG 0.544935 4:+46748772 MsaT020185.1:CDS
CATCAGAAATACATAGGGAA+AGG 0.564930 4:+46748778 MsaT020185.1:CDS
AGAAATACATAGGGAAAGGA+TGG 0.567487 4:+46748782 MsaT020185.1:CDS
GCATGCATCAGAAATACATA+GGG 0.580795 4:+46748773 MsaT020185.1:CDS
GTAACATACATCCTATTAGG+AGG 0.590541 4:-46748868 None:intergenic
GAGATTGGAGTTGAAGTCAA+TGG 0.592580 4:+46748483 MsaT020185.1:CDS
AAATCAGTCCCAGTTAACGA+CGG 0.717617 4:-46748890 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 46748477 46748909 46748477 ID=MsaG020185
Chr4 mRNA 46748477 46748909 46748477 ID=MsaT020185.1;Parent=MsaG020185
Chr4 exon 46748477 46748593 46748477 ID=MsaT020185.1.exon1;Parent=MsaT020185.1
Chr4 CDS 46748477 46748593 46748477 ID=cds.MsaT020185.1;Parent=MsaT020185.1
Chr4 exon 46748691 46748909 46748691 ID=MsaT020185.1.exon2;Parent=MsaT020185.1
Chr4 CDS 46748691 46748909 46748691 ID=cds.MsaT020185.1;Parent=MsaT020185.1
Gene Sequence

>MsaT020185.1

ATGAACGAGATTGGAGTTGAAGTCAATGGTACTGATTTAGGGAATGTTCAAAATATCGATGTTCATTATTGGAAGTATGATGTTACTACTTCTATTTTGGATGGTAACAACGTTTTGCATTTCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACTGGTAAATCTTATGACTGTGATGTGTACGCTGCGTCAAAAAGCATGCATCAGAAATACATAGGGAAAGGATGGTACGACTTTGTTCTTGAAAAGGGCCTAAAATTTGGTGACGTTATTTTGTTCACGGTCAATAATCCTCCTAATAGGATGTATGTTACCGTCGTTAACTGGGACTGA

Protein sequence

>MsaT020185.1

MNEIGVEVNGTDLGNVQNIDVHYWKYDVTTSILDGNNVLHFNQSKILLFDEETGKSYDCDVYAASKSMHQKYIGKGWYDFVLEKGLKFGDVILFTVNNPPNRMYVTVVNWD*