Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020185 | XP_039687084.1 | 79.508 | 122 | 14 | 1 | 1 | 111 | 1 | 122 | 3.09e-62 | 195 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020185 | tr|G7INX6|G7INX6_MEDTR | 79.508 | 122 | 14 | 1 | 1 | 111 | 1 | 122 | 1.47e-62 | 195 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsaG000005 | MsaG020185 | 0.801286 | 1.620320e-48 | 2.255304e-46 |
| MsaG000023 | MsaG020185 | 0.860586 | 3.666477e-63 | 2.915422e-60 |
| MsaG000049 | MsaG020185 | 0.883610 | 8.303662e-71 | 1.778266e-67 |
| MsaG000219 | MsaG020185 | 0.844189 | 1.649312e-58 | 7.358744e-56 |
| MsaG000413 | MsaG020185 | 0.809762 | 2.753215e-50 | 4.674784e-48 |
| MsaG000472 | MsaG020185 | 0.802385 | 9.662096e-49 | 1.378980e-46 |
| MsaG000545 | MsaG020185 | 0.894209 | 6.846102e-75 | 2.526290e-71 |
| MsaG000549 | MsaG020185 | 0.834357 | 5.710107e-56 | 1.876352e-53 |
| MsaG000553 | MsaG020185 | 0.869816 | 4.735456e-66 | 5.445198e-63 |
| MsaG000566 | MsaG020185 | 0.833769 | 8.006481e-56 | 2.585226e-53 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020185 | MtrunA17_Chr2g0281511 | 79.508 | 122 | 14 | 1 | 1 | 111 | 1 | 122 | 2.84e-66 | 195 |
| MsaG020185 | MtrunA17_Chr2g0309121 | 73.770 | 122 | 20 | 2 | 1 | 111 | 1 | 121 | 5.87e-59 | 177 |
| MsaG020185 | MtrunA17_Chr3g0081891 | 77.500 | 80 | 7 | 1 | 1 | 69 | 1 | 80 | 1.91e-37 | 121 |
| MsaG020185 | MtrunA17_Chr7g0276661 | 42.857 | 77 | 43 | 1 | 33 | 109 | 92 | 167 | 2.35e-17 | 72.8 |
| MsaG020185 | MtrunA17_Chr5g0430431 | 33.696 | 92 | 57 | 1 | 18 | 105 | 163 | 254 | 1.23e-13 | 64.7 |
| MsaG020185 | MtrunA17_Chr8g0356961 | 31.313 | 99 | 55 | 2 | 24 | 109 | 77 | 175 | 2.16e-11 | 57.4 |
| MsaG020185 | MtrunA17_Chr0c01g0489371 | 35.165 | 91 | 46 | 2 | 22 | 99 | 224 | 314 | 7.69e-11 | 57.4 |
Find 20 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATATCGATGTTCATTAT+TGG | 0.232793 | 4:+46748526 | MsaT020185.1:CDS |
| AAATCAGTCCCAGTTAACGA+CGG | 0.717617 | 4:-46748890 | None:intergenic |
| AAGTCAATGGTACTGATTTA+GGG | 0.335811 | 4:+46748496 | MsaT020185.1:CDS |
| ACGGTAACATACATCCTATT+AGG | 0.430681 | 4:-46748871 | None:intergenic |
| AGAAATACATAGGGAAAGGA+TGG | 0.567487 | 4:+46748782 | MsaT020185.1:CDS |
| AGCATGCATCAGAAATACAT+AGG | 0.544935 | 4:+46748772 | MsaT020185.1:CDS |
| CATCAGAAATACATAGGGAA+AGG | 0.564930 | 4:+46748778 | MsaT020185.1:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 4:+46748857 | MsaT020185.1:CDS |
| CTTGAAAAGGGCCTAAAATT+TGG | 0.185070 | 4:+46748817 | MsaT020185.1:CDS |
| GAAGTCAATGGTACTGATTT+AGG | 0.173353 | 4:+46748495 | MsaT020185.1:CDS |
CRISPR-GE
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 46748477 | 46748909 | 46748477 | ID=MsaG020185 |
| Chr4 | mRNA | 46748477 | 46748909 | 46748477 | ID=MsaT020185.1;Parent=MsaG020185 |
| Chr4 | exon | 46748477 | 46748593 | 46748477 | ID=MsaT020185.1.exon1;Parent=MsaT020185.1 |
| Chr4 | CDS | 46748477 | 46748593 | 46748477 | ID=cds.MsaT020185.1;Parent=MsaT020185.1 |
| Chr4 | exon | 46748691 | 46748909 | 46748691 | ID=MsaT020185.1.exon2;Parent=MsaT020185.1 |
| Chr4 | CDS | 46748691 | 46748909 | 46748691 | ID=cds.MsaT020185.1;Parent=MsaT020185.1 |
| Gene Sequence |
>MsaT020185.1 ATGAACGAGATTGGAGTTGAAGTCAATGGTACTGATTTAGGGAATGTTCAAAATATCGATGTTCATTATTGGAAGTATGATGTTACTACTTCTATTTTGGATGGTAACAACGTTTTGCATTTCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACTGGTAAATCTTATGACTGTGATGTGTACGCTGCGTCAAAAAGCATGCATCAGAAATACATAGGGAAAGGATGGTACGACTTTGTTCTTGAAAAGGGCCTAAAATTTGGTGACGTTATTTTGTTCACGGTCAATAATCCTCCTAATAGGATGTATGTTACCGTCGTTAACTGGGACTGA |
| Protein sequence |
>MsaT020185.1 MNEIGVEVNGTDLGNVQNIDVHYWKYDVTTSILDGNNVLHFNQSKILLFDEETGKSYDCDVYAASKSMHQKYIGKGWYDFVLEKGLKFGDVILFTVNNPPNRMYVTVVNWD* |
