AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020186

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020186 MtrunA17_Chr4g0024351 98.095 735 10 3 1 732 1 734 0.0 1479
MsaG020186 MtrunA17_Chr2g0322731 84.615 741 97 7 3 732 36 770 0.0 1251
MsaG020186 MtrunA17_Chr8g0350321 54.886 747 295 11 17 731 74 810 0.0 793
MsaG020186 MtrunA17_Chr7g0246631 57.307 698 263 11 60 731 90 778 0.0 791
MsaG020186 MtrunA17_Chr1g0203671 51.282 741 309 12 28 727 60 789 0.0 737
MsaG020186 MtrunA17_Chr6g0481041 47.411 734 334 13 44 732 2 728 0.0 678
MsaG020186 MtrunA17_Chr5g0410711 43.717 764 379 15 5 731 33 782 0.0 628
MsaG020186 MtrunA17_Chr5g0394111 48.851 696 277 12 45 727 17 646 0.0 624
MsaG020186 MtrunA17_Chr2g0331581 44.461 677 338 10 65 730 143 792 0.0 605
MsaG020186 MtrunA17_Chr6g0472471 44.415 743 358 16 28 727 35 765 0.0 599
MsaG020186 MtrunA17_Chr7g0224451 41.429 490 223 11 44 522 2 438 9.44e-123 374
MsaG020186 MtrunA17_Chr2g0292731 36.566 495 303 7 240 727 58 548 2.70e-103 327
MsaG020186 MtrunA17_Chr2g0292741 37.115 520 299 13 220 727 32 535 1.22e-101 322
MsaG020186 MtrunA17_Chr7g0224481 39.583 384 176 9 44 417 2 339 9.70e-83 266
MsaG020186 MtrunA17_Chr7g0224491 46.708 319 135 10 420 728 1 294 1.07e-80 259
MsaG020186 MtrunA17_Chr2g0292761 31.605 598 346 16 140 729 1 543 1.84e-76 256
MsaG020186 MtrunA17_Chr2g0319671 47.647 170 85 2 299 468 103 268 3.67e-48 172
MsaG020186 MtrunA17_Chr1g0205981 44.560 193 99 4 116 305 1 188 7.12e-39 142
MsaG020186 MtrunA17_Chr5g0419821 48.387 124 63 1 59 181 31 154 4.97e-36 134
MsaG020186 MtrunA17_Chr2g0319691 51.261 119 58 0 360 478 1 119 1.30e-34 129
MsaG020186 MtrunA17_Chr2g0319701 36.522 230 135 5 506 731 5 227 3.78e-34 130
MsaG020186 MtrunA17_Chr2g0319681 34.335 233 146 3 502 731 1 229 3.79e-34 130
MsaG020186 MtrunA17_Chr4g0028991 21.456 769 423 25 45 728 9 681 1.33e-22 103
MsaG020186 MtrunA17_Chr7g0224461 47.934 121 47 5 616 727 1 114 7.39e-22 91.7
MsaG020186 MtrunA17_Chr2g0326731 22.778 540 308 17 64 576 23 480 1.66e-19 93.6
MsaG020186 MtrunA17_Chr2g0304191 31.618 136 83 3 254 384 1 131 1.22e-15 74.3
MsaG020186 MtrunA17_Chr7g0224431 34.375 160 64 4 269 427 1 120 1.70e-15 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020186 AT1G05230.8 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.4 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.9 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.10 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.7 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.11 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.2 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.6 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.5 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.1 76.016 738 154 8 1 732 1 721 0.0 1127
MsaG020186 AT1G05230.3 75.745 738 154 9 1 732 1 719 0.0 1118
MsaG020186 AT4G04890.2 73.253 744 175 15 1 728 1 736 0.0 1082
MsaG020186 AT4G04890.1 73.253 744 175 15 1 728 1 736 0.0 1082
MsaG020186 AT4G21750.2 70.763 773 174 12 1 732 1 762 0.0 1071
MsaG020186 AT4G21750.1 70.763 773 174 12 1 732 1 762 0.0 1071
MsaG020186 AT4G21750.3 70.763 773 174 12 1 732 1 762 0.0 1071
MsaG020186 AT4G21750.4 70.763 773 174 12 1 732 1 762 0.0 1071
MsaG020186 AT1G05230.12 75.041 613 137 7 1 607 1 603 0.0 934
MsaG020186 AT2G32370.1 53.738 709 295 12 32 732 42 725 0.0 747
MsaG020186 AT3G61150.1 51.989 729 307 9 39 731 86 807 0.0 739
MsaG020186 AT4G00730.1 53.370 727 306 12 20 731 94 802 0.0 729
MsaG020186 AT1G73360.1 49.070 699 317 12 61 732 32 718 0.0 642
MsaG020186 AT1G17920.2 46.383 705 338 11 43 732 7 686 0.0 605
MsaG020186 AT1G17920.1 46.383 705 338 11 43 732 7 686 0.0 605
MsaG020186 AT5G52170.2 45.455 715 322 15 28 727 22 683 0.0 592
MsaG020186 AT5G52170.3 45.594 715 316 17 28 727 22 678 0.0 588
MsaG020186 AT5G52170.1 45.594 715 316 17 28 727 22 678 0.0 588
MsaG020186 AT5G46880.3 42.957 717 356 14 54 729 104 808 0.0 578
MsaG020186 AT5G46880.1 42.957 717 356 14 54 729 104 808 0.0 578
MsaG020186 AT5G46880.2 42.957 717 356 14 54 729 104 808 0.0 578
MsaG020186 AT1G79840.1 42.609 690 351 11 51 730 91 745 0.0 551
MsaG020186 AT1G79840.2 42.609 690 351 11 51 730 120 774 0.0 551
MsaG020186 AT1G79840.3 42.609 690 351 11 51 730 33 687 0.0 550
MsaG020186 AT1G79840.6 42.609 690 351 11 51 730 33 687 0.0 550
MsaG020186 AT4G17710.1 42.059 680 331 13 54 727 81 703 1.82e-174 517
MsaG020186 AT3G03260.1 39.722 720 372 19 45 728 2 695 2.74e-159 478
MsaG020186 AT1G79840.4 43.416 562 277 10 51 603 91 620 1.18e-156 468
MsaG020186 AT4G25530.1 38.955 670 379 9 61 727 40 682 1.26e-147 447
MsaG020186 AT4G00730.2 48.729 472 207 9 28 482 103 556 1.33e-146 440
MsaG020186 AT5G17320.1 36.045 713 397 18 59 730 24 718 3.97e-133 410
MsaG020186 AT1G34650.1 35.049 719 402 19 55 730 12 708 3.53e-124 387
MsaG020186 AT3G03260.2 39.602 553 296 15 45 575 2 538 8.30e-119 367
MsaG020186 AT1G79840.5 42.857 441 211 9 51 482 91 499 5.35e-111 347
MsaG020186 AT5G07260.1 28.312 551 311 21 128 664 11 491 3.81e-48 179
MsaG020186 AT5G07260.2 28.182 550 308 21 128 664 11 486 1.17e-47 177
MsaG020186 AT4G32880.1 22.594 748 411 25 64 731 15 674 5.43e-28 121
MsaG020186 AT1G52150.3 22.507 742 412 28 64 728 17 672 3.45e-25 112
MsaG020186 AT1G52150.1 22.507 742 412 28 64 728 17 672 4.12e-25 112
MsaG020186 AT1G52150.2 22.476 743 412 28 64 728 17 673 6.00e-25 111
MsaG020186 AT4G26920.1 23.191 470 253 18 254 707 72 449 9.97e-16 80.9
MsaG020186 AT5G60690.1 35.714 98 55 2 64 153 25 122 8.55e-12 69.3

Find 189 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATACTCTTCCTTCCTTCT+TGG 0.191438 4:-46801165 None:intergenic
TGATTGCCAAAACCAATTTC+TGG 0.191828 4:-46799063 None:intergenic
TCCTTCCTTTGCTTGTCATC+TGG 0.193302 4:-46798103 None:intergenic
ATTCTTACGATTCCATTTCT+TGG 0.235108 4:-46797908 None:intergenic
CTCCCACCGCGTCCAGAAAT+TGG 0.236347 4:+46799051 MsaT020186.1:CDS
TTGCAATGTGCGCCATTTCT+TGG 0.253615 4:-46801551 None:intergenic
ATGTTGCTCTGGCTCGAATT+TGG 0.272485 4:-46801868 None:intergenic
CCGCGTCCAGAAATTGGTTT+TGG 0.273136 4:+46799057 MsaT020186.1:CDS
AAGGAGTTAAGCAGAGAGTT+AGG 0.279953 4:+46798121 MsaT020186.1:CDS
CCTTCCTTTGCTTGTCATCT+GGG 0.284990 4:-46798102 None:intergenic
GCAGCTATGGAAGAGCTTAT+TGG 0.288607 4:+46799189 MsaT020186.1:CDS
CACAAATGGGTGATCCTCTT+TGG 0.301173 4:+46799217 MsaT020186.1:CDS
CATGAAGGTGGTGCTGCTTC+TGG 0.302796 4:+46797825 MsaT020186.1:CDS
TTTCGAAGCACTCGTCTTCT+CGG 0.304296 4:-46797776 None:intergenic
CAGCTATGGAAGAGCTTATT+GGG 0.306565 4:+46799190 MsaT020186.1:CDS
ACAGCTAATTCTATTATTAT+CGG 0.310525 4:-46799165 None:intergenic
CATTTAAAACCATTAGGTTT+AGG 0.310632 4:-46799318 None:intergenic
ACGATTCCATTTCTTGGATC+TGG 0.342668 4:-46797902 None:intergenic
AGCCTTTGCAAGTCAAGTTT+TGG 0.343226 4:+46798149 MsaT020186.1:CDS
ATCGACTTACATTTACTCTA+AGG 0.352335 4:-46801605 None:intergenic
AAAACTTGTGTTGGTGGAAC+AGG 0.354087 4:-46801370 None:intergenic
ATTGCAAATGGACGTGATAC+TGG 0.354415 4:+46801567 MsaT020186.1:CDS
GAGCTGATAACATGAGGTAT+AGG 0.357648 4:+46798344 MsaT020186.1:CDS
GCTATGAATGTAGTTCTAAA+TGG 0.363093 4:+46801961 MsaT020186.1:CDS
AATGGAGTCGGCGGTGAAAC+TGG 0.366633 4:+46802048 MsaT020186.1:CDS
GGAAGAAGTGCTACATAATC+TGG 0.371226 4:-46801991 None:intergenic
ACAATACCAGGAGGTCTTCC+AGG 0.379421 4:-46801319 None:intergenic
CAGAAGCAGCACCACCTTCA+TGG 0.382160 4:-46797824 None:intergenic
TATCTATGCACCGGTAGATA+TGG 0.387839 4:+46801936 MsaT020186.1:CDS
GGCAGTTGTCGATGTGTCTT+TGG 0.388385 4:+46800135 MsaT020186.1:CDS
AGATGTAGAAGAAGGCCTTC+TGG 0.388784 4:+46800184 MsaT020186.1:CDS
TATACCTTTGAGTAACCATT+TGG 0.391697 4:-46800226 None:intergenic
CTCGTGAGAGTTACTTTGTA+AGG 0.399857 4:+46800083 MsaT020186.1:CDS
AGGTCGTTTCCTCGTGGGAT+TGG 0.405874 4:+46799294 MsaT020186.1:CDS
GCTTCACATTTAAAACCATT+AGG 0.407416 4:-46799324 None:intergenic
GCTATCCTCCCTGATGGAAA+TGG 0.410049 4:+46802024 MsaT020186.1:CDS
TGTGGTGGTCCAACAGCTAT+AGG 0.411505 4:+46798397 MsaT020186.1:CDS
CTCCCTGATGGAAATGGAAA+TGG 0.415022 4:+46802030 MsaT020186.1:CDS
TATTATCGGTTTGTCTGCTT+CGG 0.415703 4:-46799151 None:intergenic
GTTAGCACTGGACATGCATT+TGG 0.416621 4:+46800866 MsaT020186.1:CDS
GGCGGTGAAACTGGCGGAGG+AGG 0.425667 4:+46802057 MsaT020186.1:CDS
CAATGGTCAACGGTGTTTGC+TGG 0.428126 4:+46799638 MsaT020186.1:CDS
TATGTAGCACTTCTTCCATC+AGG 0.428228 4:+46801997 MsaT020186.1:CDS
CTTTGCTTGTCATCTGGGTG+TGG 0.428946 4:-46798097 None:intergenic
AGCTGATAACATGAGGTATA+GGG 0.432619 4:+46798345 MsaT020186.1:CDS
GGTGAAACTGGCGGAGGAGG+AGG 0.435046 4:+46802060 MsaT020186.1:CDS
CCAACTGCTAAACTCTCTCT+TGG 0.436250 4:+46802141 MsaT020186.1:CDS
GCTGTTGGACCACCACAATT+AGG 0.438343 4:-46798391 None:intergenic
GCTTATTGGGATGGCACAAA+TGG 0.439156 4:+46799203 MsaT020186.1:CDS
GTAGTATTAACATGTTGCTC+TGG 0.442265 4:-46801879 None:intergenic
GGGATTGGACCTAAACCTAA+TGG 0.443923 4:+46799309 MsaT020186.1:CDS
ACTCCATTTCCATTTCCATC+AGG 0.444605 4:-46802033 None:intergenic
AACAACATCCTGACGGGACA+TGG 0.445961 4:+46800113 MsaT020186.1:CDS
CATTTGATGAACATCATTTG+AGG 0.446985 4:+46798428 MsaT020186.1:CDS
GGAGGAGGAGGAGCTGGTGC+TGG 0.447028 4:+46802072 MsaT020186.1:CDS
AGAATCAAAGCTTCTTTATC+CGG 0.448371 4:+46802207 MsaT020186.1:CDS
GTGTTGTCAACTGGAGTGGC+AGG 0.448507 4:+46799689 MsaT020186.1:CDS
AATGTTTCAGTCAAACCTCT+TGG 0.448780 4:+46797689 None:intergenic
CGTAAGAGTGTCGATGATCC+TGG 0.451139 4:+46801301 MsaT020186.1:CDS
CACTCGTCTTCTCGGATTCG+AGG 0.451326 4:-46797768 None:intergenic
TATTTGTTGCAGTTTCTTCA+AGG 0.453245 4:+46798069 MsaT020186.1:intron
AGAATTAGCTGTTGCAGCTA+TGG 0.453837 4:+46799176 MsaT020186.1:CDS
GAGCCAGTGGAGATATTCTA+AGG 0.455723 4:+46799112 MsaT020186.1:CDS
CGGTGGAGTTGTCCAAGAAA+TGG 0.457380 4:+46801539 MsaT020186.1:CDS
AAGGAAGAGTATGTTGAAAC+TGG 0.460628 4:+46801174 MsaT020186.1:CDS
TGCATTTGGTGCAAAACGTT+GGG 0.460920 4:+46800880 MsaT020186.1:CDS
CAGTCAAACCTCTTGGAAGC+TGG 0.469434 4:+46797696 MsaT020186.1:CDS
CACACATGGACAACATTGTC+TGG 0.470640 4:+46801247 MsaT020186.1:CDS
TCAGCACCAGATCCAAGAAA+TGG 0.471078 4:+46797896 MsaT020186.1:CDS
TGGACAACATTGTCTGGAAC+AGG 0.473333 4:+46801253 MsaT020186.1:CDS
GAAATGGCGCACATTGCAAA+TGG 0.473589 4:+46801555 MsaT020186.1:CDS
ACTGGCGGAGGAGGAGGAGC+TGG 0.473807 4:+46802066 MsaT020186.1:CDS
GGAGGAGGAGCTGGTGCTGG+TGG 0.478826 4:+46802075 MsaT020186.1:CDS
CTACATCTAGATGAAGGACT+GGG 0.481691 4:-46800169 None:intergenic
CTCCATTTCCATTTCCATCA+GGG 0.484372 4:-46802032 None:intergenic
GTTGAAACTGGCAGAGAGAA+TGG 0.485726 4:+46801186 MsaT020186.1:CDS
GACATTTCACCTATAGCTGT+TGG 0.486224 4:-46798406 None:intergenic
TTAATGAAGATGAATATGTT+AGG 0.488862 4:+46799274 MsaT020186.1:CDS
CTCTCACGAGTCGGCACAAG+AGG 0.489363 4:-46800070 None:intergenic
ACAACTGCCCATGTCCCGTC+AGG 0.494676 4:-46800121 None:intergenic
GCAGACACAACATGAACGTT+CGG 0.496911 4:+46798285 MsaT020186.1:intron
ATGTTAGGTCGTTTCCTCGT+GGG 0.513468 4:+46799289 MsaT020186.1:CDS
AGCACAGTAGCAGCTCCATC+AGG 0.513971 4:-46799252 None:intergenic
ATTGGTACAATGGACATGTA+TGG 0.514146 4:+46799090 MsaT020186.1:CDS
AGGAGTTAAGCAGAGAGTTA+GGG 0.515842 4:+46798122 MsaT020186.1:CDS
CTCATTTGTTATCTATGCAC+CGG 0.516034 4:+46801927 MsaT020186.1:CDS
GAGAGTGTAACAGATGCAAC+AGG 0.516631 4:+46801904 MsaT020186.1:CDS
CGATGGCAGCGAAGTACGTA+GGG 0.519809 4:+46798992 MsaT020186.1:CDS
ACTGTGGAAGTGTTGTCAAC+TGG 0.520701 4:+46799680 MsaT020186.1:CDS
AACCAAAACTTGACTTGCAA+AGG 0.523378 4:-46798151 None:intergenic
ATCGGTTTGTCTGCTTCGGT+CGG 0.525191 4:-46799147 None:intergenic
CAGCAAACACCGTTGACCAT+TGG 0.528564 4:-46799637 None:intergenic
TACAAGCAGATTGTTAGCAC+TGG 0.528579 4:+46800854 MsaT020186.1:CDS
AAACAAAAGGACTCAGATGA+AGG 0.533633 4:+46798177 MsaT020186.1:CDS
AGTGGGAATGTTTGTTGCCA+TGG 0.534197 4:-46800942 None:intergenic
CTTATTGGGATGGCACAAAT+GGG 0.536780 4:+46799204 MsaT020186.1:CDS
TTGATTCAAGAGATGCCAAA+TGG 0.538963 4:+46800211 MsaT020186.1:CDS
TTTGAAAAGCGACGGTCAGA+AGG 0.541068 4:-46802101 None:intergenic
GATATTCTAAGGTCAATTAG+TGG 0.541194 4:+46799123 MsaT020186.1:CDS
ATGTTTGAATAGTTTACCAC+AGG 0.541643 4:-46799018 None:intergenic
CCAAAACCAATTTCTGGACG+CGG 0.541993 4:-46799057 None:intergenic
CTTCATCTAGATGTAGAAGA+AGG 0.545581 4:+46800176 MsaT020186.1:CDS
GATGATCCTGGAAGACCTCC+TGG 0.545888 4:+46801313 MsaT020186.1:CDS
TTTGCATCATCTCTAATGGA+TGG 0.546858 4:-46797725 None:intergenic
GAAGACGAGTGCTTCGAAAG+TGG 0.546911 4:+46797780 MsaT020186.1:CDS
TCTGGACGCGGTGGGAGAGA+TGG 0.548949 4:-46799045 None:intergenic
TTTCCAACATATAGGTGACA+TGG 0.550283 4:+46800789 MsaT020186.1:intron
CTTCTTCTACATCTAGATGA+AGG 0.552711 4:-46800175 None:intergenic
TCTACATCTAGATGAAGGAC+TGG 0.555194 4:-46800170 None:intergenic
TGGTTAAGTACTACACCTGA+TGG 0.558663 4:+46799237 MsaT020186.1:CDS
CAATCAAGGGATTGGTACAA+TGG 0.559479 4:+46799080 MsaT020186.1:CDS
AACATTCCCACTGTCGATGT+TGG 0.559966 4:+46800953 MsaT020186.1:CDS
CGAAAGGCTTGCTAGCTCCA+TGG 0.560399 4:+46800925 MsaT020186.1:CDS
CTTGGATCTGGTGCTGAGTG+TGG 0.562215 4:-46797890 None:intergenic
ATTCCCACTGTCGATGTTGG+CGG 0.565704 4:+46800956 MsaT020186.1:CDS
ATGCATTTGGTGCAAAACGT+TGG 0.567718 4:+46800879 MsaT020186.1:CDS
CTTTAGATCGACAATGCGAA+AGG 0.567913 4:+46800909 MsaT020186.1:CDS
TTCATAGCAACCATATCTAC+CGG 0.568044 4:-46801946 None:intergenic
CAGTGGGATATTCTTTCTAA+CGG 0.572964 4:+46801519 MsaT020186.1:intron
CCAAGAGAGAGTTTAGCAGT+TGG 0.575737 4:-46802141 None:intergenic
ATTTGAGGCTTGAAAATGCA+AGG 0.576070 4:+46798443 MsaT020186.1:CDS
TAACCTTGTTGAGATTCTCA+TGG 0.576995 4:+46799383 MsaT020186.1:CDS
GGGAGGATAGCAAATCCTGA+TGG 0.577076 4:-46802012 None:intergenic
TTCCAACATATAGGTGACAT+GGG 0.577979 4:+46800790 MsaT020186.1:intron
TGTTGAGATTCTCATGGATG+TGG 0.578448 4:+46799389 MsaT020186.1:CDS
TGACCTTAGAATATCTCCAC+TGG 0.581106 4:-46799115 None:intergenic
ACTCGTCTTCTCGGATTCGA+GGG 0.584124 4:-46797767 None:intergenic
GCTCAACTAAGCACTTTCAC+CGG 0.585345 4:-46802226 None:intergenic
AACATGTAGAAGTGGATGAG+AGG 0.585664 4:+46800816 MsaT020186.1:CDS
GGATTTGCTATCCTCCCTGA+TGG 0.589528 4:+46802018 MsaT020186.1:CDS
TATGTTAGGTCGTTTCCTCG+TGG 0.589599 4:+46799288 MsaT020186.1:CDS
AACCAAGATTTGAAAAGCGA+CGG 0.590397 4:-46802109 None:intergenic
TTAGTGATAACAAACCAAGA+AGG 0.592109 4:+46801151 MsaT020186.1:intron
GATGGAAATGGAAATGGAGT+CGG 0.592474 4:+46802036 MsaT020186.1:CDS
AGAATGGTTATAAGTTTCTG+TGG 0.594648 4:+46801202 MsaT020186.1:CDS
ACAACATCCTGACGGGACAT+GGG 0.595244 4:+46800114 MsaT020186.1:CDS
ACGATGGCAGCGAAGTACGT+AGG 0.596635 4:+46798991 MsaT020186.1:CDS
GTGCTTCAACAGCACACACA+TGG 0.598437 4:+46801233 MsaT020186.1:CDS
ATTGTAAACAACATCCTGAC+GGG 0.600101 4:+46800107 MsaT020186.1:CDS
CCCAGATGACAAGCAAAGGA+AGG 0.600252 4:+46798102 MsaT020186.1:CDS
GATGCCAAATGGTTACTCAA+AGG 0.601554 4:+46800222 MsaT020186.1:CDS
TGTTTCAAGAGCTGTGACTG+TGG 0.603466 4:+46799664 MsaT020186.1:CDS
TGATAACAAACCAAGAAGGA+AGG 0.604066 4:+46801155 MsaT020186.1:CDS
ATGGTTATAAGTTTCTGTGG+TGG 0.605079 4:+46801205 MsaT020186.1:CDS
TCTTGAATCAAGCAACCAGA+AGG 0.605320 4:-46800199 None:intergenic
AGTCATCACTCTCACATCAT+CGG 0.612975 4:-46801278 None:intergenic
ATATTCTAAGGTCAATTAGT+GGG 0.613763 4:+46799124 MsaT020186.1:CDS
TATGGAAGAGCTTATTGGGA+TGG 0.613912 4:+46799194 MsaT020186.1:CDS
ACATGTAGAAGTGGATGAGA+GGG 0.614639 4:+46800817 MsaT020186.1:CDS
TTGAAAAGCGACGGTCAGAA+GGG 0.614971 4:-46802100 None:intergenic
CTACAATGGTGCTTTACAAG+TGG 0.618320 4:+46799715 MsaT020186.1:CDS
AAATGCAAGGCTAAGAGAAG+AGG 0.619130 4:+46798456 MsaT020186.1:CDS
AAACCAATTTCTGGACGCGG+TGG 0.619643 4:-46799054 None:intergenic
CGAGTCGGCACAAGAGGTGA+AGG 0.619674 4:-46800064 None:intergenic
CACACCCAGATGACAAGCAA+AGG 0.622325 4:+46798098 MsaT020186.1:CDS
GTCGGCGGTGAAACTGGCGG+AGG 0.626051 4:+46802054 MsaT020186.1:CDS
GCTGCTTCTGGTGAAGAACA+AGG 0.627125 4:+46797837 MsaT020186.1:CDS
TCCTGCAGTGAAAACCATGA+AGG 0.632264 4:+46797810 MsaT020186.1:CDS
AACCAATTTCTGGACGCGGT+GGG 0.632731 4:-46799053 None:intergenic
ATGGACATGTATGGAGCCAG+TGG 0.634074 4:+46799099 MsaT020186.1:CDS
ATGGGTTGAACATGTAGAAG+TGG 0.635474 4:+46800808 MsaT020186.1:CDS
ATGAATGTAGTTCTAAATGG+AGG 0.639925 4:+46801964 MsaT020186.1:CDS
TATTGTAAACAACATCCTGA+CGG 0.640849 4:+46800106 MsaT020186.1:CDS
TGTTGTCAACTGGAGTGGCA+GGG 0.641410 4:+46799690 MsaT020186.1:CDS
GGAGTCGGCGGTGAAACTGG+CGG 0.641968 4:+46802051 MsaT020186.1:CDS
GCTTCATGTCCTAATTGTGG+TGG 0.643891 4:+46798382 MsaT020186.1:CDS
GGATCTGGTGCTGAGTGTGG+CGG 0.645442 4:-46797887 None:intergenic
ACAAAGTAACTCTCACGAGT+CGG 0.646006 4:-46800079 None:intergenic
AGCTTAGAGCTGATAACATG+AGG 0.647699 4:+46798338 MsaT020186.1:CDS
TGGGATATTCTTTCTAACGG+TGG 0.650199 4:+46801522 MsaT020186.1:CDS
GCAGCACTAAGAACAATACC+AGG 0.650728 4:-46801331 None:intergenic
GGTGTAGTACTTAACCAAAG+AGG 0.657817 4:-46799231 None:intergenic
AATGCTTCATGTCCTAATTG+TGG 0.661151 4:+46798379 MsaT020186.1:CDS
TATACCGCCAACATCGACAG+TGG 0.664674 4:-46800960 None:intergenic
CATTTCCATTTCCATCAGGG+AGG 0.666915 4:-46802029 None:intergenic
GGAACTGACCAGCTTCCAAG+AGG 0.667277 4:-46797704 None:intergenic
GATTGATAGAATTTCAACGA+TGG 0.671483 4:+46798975 MsaT020186.1:intron
ATACCGCCAACATCGACAGT+GGG 0.671676 4:-46800959 None:intergenic
GGAAGTGTTGTCAACTGGAG+TGG 0.673358 4:+46799685 MsaT020186.1:CDS
CATCCATGAGAATCTCAACA+AGG 0.674971 4:-46799386 None:intergenic
GGAGTGGCAGGGAACTACAA+TGG 0.679417 4:+46799701 MsaT020186.1:CDS
GAACGAGAGCTCCAGAACTG+AGG 0.682761 4:+46801405 MsaT020186.1:CDS
GGAAATGGAAATGGAGTCGG+CGG 0.694782 4:+46802039 MsaT020186.1:CDS
CATGTAGAAGTGGATGAGAG+GGG 0.694795 4:+46800818 MsaT020186.1:CDS
TGCAGTGAAAACCATGAAGG+TGG 0.700352 4:+46797813 MsaT020186.1:CDS
TACATCTAGATGAAGGACTG+GGG 0.707753 4:-46800168 None:intergenic
GAAGTACGTAGGGAAGCCTG+TGG 0.754472 4:+46799002 MsaT020186.1:CDS
GGTTTAGGTCCAATCCCACG+AGG 0.756148 4:-46799303 None:intergenic
GCACTAAGAACAATACCAGG+AGG 0.759585 4:-46801328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 46797690 46802242 46797690 ID=MsaG020186
Chr4 mRNA 46797690 46802242 46797690 ID=MsaT020186.1;Parent=MsaG020186
Chr4 exon 46797690 46797922 46797690 ID=MsaT020186.1.exon1;Parent=MsaT020186.1
Chr4 CDS 46797690 46797922 46797690 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46798081 46798198 46798081 ID=MsaT020186.1.exon2;Parent=MsaT020186.1
Chr4 CDS 46798081 46798198 46798081 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46798289 46798477 46798289 ID=MsaT020186.1.exon3;Parent=MsaT020186.1
Chr4 CDS 46798289 46798477 46798289 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46798976 46799410 46798976 ID=MsaT020186.1.exon4;Parent=MsaT020186.1
Chr4 CDS 46798976 46799410 46798976 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46799635 46799736 46799635 ID=MsaT020186.1.exon5;Parent=MsaT020186.1
Chr4 CDS 46799635 46799736 46799635 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46800043 46800243 46800043 ID=MsaT020186.1.exon6;Parent=MsaT020186.1
Chr4 CDS 46800043 46800243 46800043 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46800803 46800977 46800803 ID=MsaT020186.1.exon7;Parent=MsaT020186.1
Chr4 CDS 46800803 46800977 46800803 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46801155 46801426 46801155 ID=MsaT020186.1.exon8;Parent=MsaT020186.1
Chr4 CDS 46801155 46801426 46801155 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46801522 46801617 46801522 ID=MsaT020186.1.exon9;Parent=MsaT020186.1
Chr4 CDS 46801522 46801617 46801522 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Chr4 exon 46801865 46802242 46801865 ID=MsaT020186.1.exon10;Parent=MsaT020186.1
Chr4 CDS 46801865 46802242 46801865 ID=cds.MsaT020186.1;Parent=MsaT020186.1
Gene Sequence

>MsaT020186.1

ATGTTTCAGTCAAACCTCTTGGAAGCTGGTCAGTTCCATCCATTAGAGATGATGCAAAACACTTCAGAAAGCGATGTCCCTCGAATCCGAGAAGACGAGTGCTTCGAAAGTGGCACCAAATCCTGCAGTGAAAACCATGAAGGTGGTGCTGCTTCTGGTGAAGAACAAGGACCACGTCCCAAAAGAAAACGTTATCACCGCCACACTCAGCACCAGATCCAAGAAATGGAATCTTTCTTCAAGGAGTGTCCACACCCAGATGACAAGCAAAGGAAGGAGTTAAGCAGAGAGTTAGGGCTAGAGCCTTTGCAAGTCAAGTTTTGGTTTCAAAACAAAAGGACTCAGATGAAGACACAACATGAACGTTCGGAGAATTCTCAACTAAGAGCAGAAAATGAAAAGCTTAGAGCTGATAACATGAGGTATAGGGAAGCTCTTAGCAATGCTTCATGTCCTAATTGTGGTGGTCCAACAGCTATAGGTGAAATGTCATTTGATGAACATCATTTGAGGCTTGAAAATGCAAGGCTAAGAGAAGAGATTGATAGAATTTCAACGATGGCAGCGAAGTACGTAGGGAAGCCTGTGGTAAACTATTCAAACATTTCACCATCTCTCCCACCGCGTCCAGAAATTGGTTTTGGCAATCAAGGGATTGGTACAATGGACATGTATGGAGCCAGTGGAGATATTCTAAGGTCAATTAGTGGGCCGACCGAAGCAGACAAACCGATAATAATAGAATTAGCTGTTGCAGCTATGGAAGAGCTTATTGGGATGGCACAAATGGGTGATCCTCTTTGGTTAAGTACTACACCTGATGGAGCTGCTACTGTGCTTAATGAAGATGAATATGTTAGGTCGTTTCCTCGTGGGATTGGACCTAAACCTAATGGTTTTAAATGTGAAGCTTCGAGGGAATCTAGCGTTGTTATCATGAATCATGTTAACCTTGTTGAGATTCTCATGGATGTGAACCAATGGTCAACGGTGTTTGCTGGTATTGTTTCAAGAGCTGTGACTGTGGAAGTGTTGTCAACTGGAGTGGCAGGGAACTACAATGGTGCTTTACAAGTGATGACAGCAGAGTTTCAAGTACCTTCACCTCTTGTGCCGACTCGTGAGAGTTACTTTGTAAGGTATTGTAAACAACATCCTGACGGGACATGGGCAGTTGTCGATGTGTCTTTGGATAATCTGCGCCCCAGTCCTTCATCTAGATGTAGAAGAAGGCCTTCTGGTTGCTTGATTCAAGAGATGCCAAATGGTTACTCAAAGGTGACATGGGTTGAACATGTAGAAGTGGATGAGAGGGGTGTTCATAATCTATACAAGCAGATTGTTAGCACTGGACATGCATTTGGTGCAAAACGTTGGGTTGCAACTTTAGATCGACAATGCGAAAGGCTTGCTAGCTCCATGGCAACAAACATTCCCACTGTCGATGTTGGCGTGATAACAAACCAAGAAGGAAGGAAGAGTATGTTGAAACTGGCAGAGAGAATGGTTATAAGTTTCTGTGGTGGAGTGAGTGCTTCAACAGCACACACATGGACAACATTGTCTGGAACAGGAGCCGATGATGTGAGAGTGATGACTCGTAAGAGTGTCGATGATCCTGGAAGACCTCCTGGTATTGTTCTTAGTGCTGCTACTTCTTTTTGGCTTCCTGTTCCACCAACACAAGTTTTTGAATTTCTTCGGAACGAGAGCTCCAGAACTGAGTGGGATATTCTTTCTAACGGTGGAGTTGTCCAAGAAATGGCGCACATTGCAAATGGACGTGATACTGGAAACTGTGTCTCTCTCCTTAGAGTAAATAGTCCAAATTCGAGCCAGAGCAACATGTTAATACTACAAGAGAGTGTAACAGATGCAACAGGCTCATTTGTTATCTATGCACCGGTAGATATGGTTGCTATGAATGTAGTTCTAAATGGAGGAGATCCAGATTATGTAGCACTTCTTCCATCAGGATTTGCTATCCTCCCTGATGGAAATGGAAATGGAGTCGGCGGTGAAACTGGCGGAGGAGGAGGAGCTGGTGCTGGTGGATCCCTTCTGACCGTCGCTTTTCAAATCTTGGTTGATTCTACTCCAACTGCTAAACTCTCTCTTGGATCAGTTGCTACTGTTAATAGTCTTATTGCTTGTACTGTCGAGAGAATCAAAGCTTCTTTATCCGGTGAAAGTGCTTAG
Protein sequence

>MsaT020186.1

MFQSNLLEAGQFHPLEMMQNTSESDVPRIREDECFESGTKSCSENHEGGAASGEEQGPRPKRKRYHRHTQHQIQEMESFFKECPHPDDKQRKELSRELGLEPLQVKFWFQNKRTQMKTQHERSENSQLRAENEKLRADNMRYREALSNASCPNCGGPTAIGEMSFDEHHLRLENARLREEIDRISTMAAKYVGKPVVNYSNISPSLPPRPEIGFGNQGIGTMDMYGASGDILRSISGPTEADKPIIIELAVAAMEELIGMAQMGDPLWLSTTPDGAATVLNEDEYVRSFPRGIGPKPNGFKCEASRESSVVIMNHVNLVEILMDVNQWSTVFAGIVSRAVTVEVLSTGVAGNYNGALQVMTAEFQVPSPLVPTRESYFVRYCKQHPDGTWAVVDVSLDNLRPSPSSRCRRRPSGCLIQEMPNGYSKVTWVEHVEVDERGVHNLYKQIVSTGHAFGAKRWVATLDRQCERLASSMATNIPTVDVGVITNQEGRKSMLKLAERMVISFCGGVSASTAHTWTTLSGTGADDVRVMTRKSVDDPGRPPGIVLSAATSFWLPVPPTQVFEFLRNESSRTEWDILSNGGVVQEMAHIANGRDTGNCVSLLRVNSPNSSQSNMLILQESVTDATGSFVIYAPVDMVAMNVVLNGGDPDYVALLPSGFAILPDGNGNGVGGETGGGGGAGAGGSLLTVAFQILVDSTPTAKLSLGSVATVNSLIACTVERIKASLSGESA*