AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020242

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020242 MtrunA17_Chr4g0025061 84.261 629 81 4 1 629 1 611 0.0 1010
MsaG020242 MtrunA17_Chr4g0025051 76.801 569 124 4 32 600 7 567 0.0 810
MsaG020242 MtrunA17_Chr4g0024391 73.239 497 124 4 133 629 1 488 0.0 681
MsaG020242 MtrunA17_Chr2g0322391 44.397 464 174 14 205 606 150 591 7.93e-93 299
MsaG020242 MtrunA17_Chr2g0288871 36.727 550 237 15 60 581 67 533 1.42e-82 270
MsaG020242 MtrunA17_Chr4g0075201 57.018 228 84 6 344 565 245 464 1.17e-71 240
MsaG020242 MtrunA17_Chr3g0099641 47.479 238 95 5 346 577 231 444 2.29e-63 217
MsaG020242 MtrunA17_Chr7g0270831 46.124 258 95 7 351 602 201 420 2.50e-56 197
MsaG020242 MtrunA17_Chr2g0302391 48.529 204 90 5 351 542 196 396 4.23e-47 172
MsaG020242 MtrunA17_Chr4g0024401 70.833 120 29 2 16 135 8 121 1.72e-46 160
MsaG020242 MtrunA17_Chr7g0216891 51.613 124 54 1 323 440 170 293 3.27e-37 146
MsaG020242 MtrunA17_Chr4g0051181 37.453 267 135 6 351 592 228 487 2.66e-35 139
MsaG020242 MtrunA17_Chr4g0060271 45.550 191 73 5 327 486 198 388 1.22e-32 133
MsaG020242 MtrunA17_Chr3g0101131 44.037 109 57 2 339 444 388 495 1.19e-24 108
MsaG020242 MtrunA17_Chr5g0418191 50.515 97 43 2 336 428 286 381 1.89e-24 106
MsaG020242 MtrunA17_Chr5g0418191 36.111 108 65 3 367 474 152 255 4.53e-11 65.5
MsaG020242 MtrunA17_Chr7g0218081 48.454 97 49 1 342 438 386 481 3.40e-24 107
MsaG020242 MtrunA17_Chr5g0445311 55.000 80 35 1 349 428 347 425 8.86e-24 105
MsaG020242 MtrunA17_Chr4g0001361 35.099 151 74 4 343 493 139 265 2.37e-23 101
MsaG020242 MtrunA17_Chr5g0432161 48.387 93 47 1 336 428 391 482 3.98e-23 103
MsaG020242 MtrunA17_Chr4g0042831 51.852 81 38 1 348 428 260 339 6.89e-23 101
MsaG020242 MtrunA17_Chr3g0079691 54.321 81 36 1 348 428 477 556 1.09e-22 103
MsaG020242 MtrunA17_Chr3g0079691 43.750 64 34 2 367 430 281 342 3.38e-11 66.6
MsaG020242 MtrunA17_Chr3g0106491 46.316 95 50 1 333 427 332 425 1.94e-22 101
MsaG020242 MtrunA17_Chr3g0106491 40.230 87 46 3 344 430 180 260 3.38e-12 69.3
MsaG020242 MtrunA17_Chr7g0243891 54.321 81 36 1 348 428 356 435 6.54e-22 100
MsaG020242 MtrunA17_Chr4g0004911 53.165 79 36 1 348 426 318 395 7.91e-22 99.4
MsaG020242 MtrunA17_Chr4g0004911 40.698 86 44 3 350 433 170 250 1.72e-11 67.0
MsaG020242 MtrunA17_Chr7g0249301 53.947 76 34 1 353 428 279 353 1.13e-21 97.4
MsaG020242 MtrunA17_Chr4g0051881 41.667 108 62 1 353 460 165 271 2.83e-21 95.5
MsaG020242 MtrunA17_Chr5g0419241 66.038 53 18 0 344 396 55 107 2.94e-21 89.4
MsaG020242 MtrunA17_Chr7g0249311 50.617 81 39 1 348 428 374 453 3.47e-21 98.2
MsaG020242 MtrunA17_Chr7g0270191 45.745 94 46 2 351 444 130 218 3.51e-21 94.4
MsaG020242 MtrunA17_Chr3g0136961 41.837 98 56 1 327 423 98 195 5.38e-21 94.0
MsaG020242 MtrunA17_Chr5g0447701 51.807 83 38 2 347 428 232 313 5.98e-21 95.5
MsaG020242 MtrunA17_Chr2g0333851 50.000 78 37 1 352 427 153 230 9.26e-21 93.6
MsaG020242 MtrunA17_Chr1g0150271 50.000 66 33 0 361 426 96 161 1.24e-20 92.0
MsaG020242 MtrunA17_Chr1g0150281 44.565 92 51 0 351 442 125 216 2.30e-20 92.0
MsaG020242 MtrunA17_Chr1g0150801 52.703 74 34 1 351 424 87 159 1.02e-19 86.7
MsaG020242 MtrunA17_Chr1g0147881 56.716 67 29 0 367 433 263 329 1.29e-19 90.9
MsaG020242 MtrunA17_Chr2g0294231 47.500 80 41 1 351 430 188 266 1.78e-19 90.9
MsaG020242 MtrunA17_Chr7g0263531 42.857 98 45 2 329 426 334 420 1.97e-19 91.7
MsaG020242 MtrunA17_Chr7g0263531 44.444 72 38 2 367 438 190 259 1.41e-11 67.0
MsaG020242 MtrunA17_Chr4g0053281 56.452 62 27 0 367 428 267 328 4.10e-19 89.4
MsaG020242 MtrunA17_Chr8g0386241 36.697 109 68 1 324 432 61 168 5.39e-19 85.1
MsaG020242 MtrunA17_Chr1g0194481 44.444 90 45 2 353 442 145 229 8.82e-19 88.2
MsaG020242 MtrunA17_Chr7g0224801 52.857 70 32 1 356 425 83 151 1.29e-18 84.0
MsaG020242 MtrunA17_Chr8g0379461 53.968 63 29 0 366 428 76 138 1.45e-18 85.5
MsaG020242 MtrunA17_Chr2g0293481 32.184 174 96 6 283 436 166 337 1.58e-18 89.0
MsaG020242 MtrunA17_Chr5g0401821 56.667 60 26 0 366 425 199 258 1.74e-18 85.9
MsaG020242 MtrunA17_Chr7g0270051 42.857 91 49 1 362 452 49 136 2.20e-18 85.5
MsaG020242 MtrunA17_Chr2g0319961 46.835 79 42 0 357 435 71 149 5.18e-18 84.3
MsaG020242 MtrunA17_Chr1g0184691 57.895 57 24 0 367 423 294 350 5.58e-18 86.3
MsaG020242 MtrunA17_Chr5g0443131 47.436 78 40 1 353 430 144 220 8.83e-18 84.7
MsaG020242 MtrunA17_Chr2g0289181 45.455 77 42 0 361 437 9 85 1.23e-17 82.8
MsaG020242 MtrunA17_Chr7g0248721 45.263 95 47 3 353 447 128 217 1.76e-17 82.0
MsaG020242 MtrunA17_Chr2g0284321 51.562 64 31 0 363 426 32 95 1.89e-17 81.6
MsaG020242 MtrunA17_Chr4g0068541 47.368 76 39 1 352 427 194 268 2.69e-17 84.7
MsaG020242 MtrunA17_Chr4g0074001 43.158 95 53 1 336 430 329 422 3.22e-17 85.1
MsaG020242 MtrunA17_Chr8g0335811 48.101 79 40 1 345 423 133 210 3.91e-17 81.3
MsaG020242 MtrunA17_Chr7g0248691 48.000 75 39 0 362 436 150 224 4.73e-17 82.8
MsaG020242 MtrunA17_Chr1g0188941 50.000 66 32 1 361 426 46 110 9.62e-17 76.6
MsaG020242 MtrunA17_Chr3g0129361 43.478 92 50 2 353 444 131 220 1.02e-16 79.7
MsaG020242 MtrunA17_Chr8g0348311 46.667 75 40 0 362 436 151 225 1.66e-16 81.3
MsaG020242 MtrunA17_Chr8g0383251 44.156 77 42 1 353 429 95 170 3.21e-16 77.4
MsaG020242 MtrunA17_Chr4g0071521 53.448 58 27 0 366 423 245 302 3.30e-16 80.1
MsaG020242 MtrunA17_Chr8g0387501 44.444 72 40 0 365 436 128 199 3.33e-16 79.3
MsaG020242 MtrunA17_Chr5g0418781 47.436 78 40 1 346 423 104 180 3.59e-16 79.3
MsaG020242 MtrunA17_Chr8g0383351 39.000 100 59 2 325 423 118 216 2.94e-15 75.9
MsaG020242 MtrunA17_Chr1g0202461 40.860 93 53 1 364 456 259 349 8.39e-15 76.6
MsaG020242 MtrunA17_Chr3g0129321 45.333 75 40 1 353 427 105 178 1.26e-14 73.2
MsaG020242 MtrunA17_Chr7g0228941 42.029 69 39 1 355 423 42 109 3.19e-14 69.7
MsaG020242 MtrunA17_Chr1g0189391 47.222 72 36 2 353 423 84 154 8.75e-14 73.2
MsaG020242 MtrunA17_Chr6g0485431 46.250 80 41 2 353 431 141 219 1.32e-13 72.8
MsaG020242 MtrunA17_Chr3g0139021 42.029 69 39 1 355 423 304 371 7.72e-13 70.9
MsaG020242 MtrunA17_Chr7g0238921 50.877 57 27 1 367 423 125 180 9.49e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020242 AT4G04450.1 39.962 528 225 15 65 577 53 503 6.03e-85 276
MsaG020242 AT4G04450.2 40.919 479 198 14 114 577 1 409 1.69e-80 261
MsaG020242 AT1G62300.1 50.562 267 109 8 345 593 291 552 2.17e-66 228
MsaG020242 AT4G22070.4 54.941 253 89 8 345 581 276 519 1.51e-61 214
MsaG020242 AT4G22070.3 54.941 253 89 8 345 581 276 519 1.51e-61 214
MsaG020242 AT4G22070.2 54.941 253 89 8 345 581 276 519 1.51e-61 214
MsaG020242 AT4G22070.1 54.941 253 89 8 345 581 276 519 1.51e-61 214
MsaG020242 AT4G01720.1 43.051 295 127 8 300 574 170 443 2.79e-46 171
MsaG020242 AT5G15130.2 36.162 271 122 9 346 578 165 422 1.90e-38 149
MsaG020242 AT5G15130.1 36.162 271 122 9 346 578 207 464 2.14e-38 150
MsaG020242 AT1G68150.1 48.299 147 69 2 304 447 174 316 2.46e-38 146
MsaG020242 AT1G69810.2 46.023 176 84 5 320 494 103 268 2.03e-36 139
MsaG020242 AT1G69810.1 46.023 176 84 5 320 494 161 326 2.54e-36 140
MsaG020242 AT1G18860.1 30.570 386 201 12 139 504 48 386 9.93e-34 136
MsaG020242 AT2G03340.1 44.828 116 54 2 313 428 371 476 2.50e-24 107
MsaG020242 AT2G03340.1 51.667 60 27 2 367 426 251 308 1.24e-11 68.2
MsaG020242 AT1G13960.2 55.696 79 34 1 348 426 238 315 5.11e-24 104
MsaG020242 AT1G13960.2 47.222 72 36 2 367 438 77 146 7.55e-12 67.8
MsaG020242 AT4G26640.5 52.500 80 37 1 349 428 188 266 5.66e-24 104
MsaG020242 AT4G26640.6 52.500 80 37 1 349 428 188 266 5.66e-24 104
MsaG020242 AT4G26640.4 52.500 80 37 1 349 428 188 266 5.66e-24 104
MsaG020242 AT4G26640.3 52.500 80 37 1 349 428 188 266 5.66e-24 104
MsaG020242 AT1G13960.1 55.696 79 34 1 348 426 391 468 6.49e-24 106
MsaG020242 AT1G13960.1 47.222 72 36 2 367 438 230 299 5.98e-12 68.9
MsaG020242 AT2G38470.1 54.321 81 36 1 348 428 344 423 1.35e-23 105
MsaG020242 AT4G26640.7 52.500 80 37 1 349 428 324 402 1.61e-23 105
MsaG020242 AT4G26640.1 52.500 80 37 1 349 428 292 370 1.72e-23 104
MsaG020242 AT4G26640.2 52.500 80 37 1 349 428 364 442 2.26e-23 105
MsaG020242 AT4G31800.2 43.810 105 54 2 345 444 85 189 2.15e-22 97.4
MsaG020242 AT4G31800.1 43.810 105 54 2 345 444 150 254 3.00e-22 98.6
MsaG020242 AT5G56270.1 53.086 81 37 1 348 428 469 548 6.81e-22 100
MsaG020242 AT2G04880.2 45.055 91 49 1 336 426 253 342 1.02e-21 99.4
MsaG020242 AT2G04880.2 49.275 69 31 3 362 429 107 172 9.03e-12 68.2
MsaG020242 AT3G01080.2 48.837 86 43 1 341 426 319 403 1.08e-21 99.0
MsaG020242 AT3G01080.1 48.837 86 43 1 341 426 281 365 1.16e-21 98.6
MsaG020242 AT2G25000.3 34.091 176 103 4 266 429 14 188 1.34e-21 95.1
MsaG020242 AT2G04880.1 45.055 91 49 1 336 426 277 366 1.44e-21 99.0
MsaG020242 AT2G04880.1 49.275 69 31 3 362 429 107 172 1.04e-11 68.2
MsaG020242 AT1G80840.1 47.436 78 39 1 352 427 130 207 3.16e-21 95.1
MsaG020242 AT1G29860.1 46.809 94 45 2 351 444 121 209 4.01e-21 94.4
MsaG020242 AT5G07100.2 48.235 85 43 1 346 430 121 204 4.51e-21 92.8
MsaG020242 AT5G07100.4 48.235 85 43 1 346 430 121 204 4.51e-21 92.8
MsaG020242 AT5G07100.5 48.235 85 43 1 346 430 121 204 4.51e-21 92.8
MsaG020242 AT5G07100.3 48.235 85 43 1 346 430 121 204 4.51e-21 92.8
MsaG020242 AT5G07100.1 48.235 85 43 1 346 430 214 297 8.65e-21 94.0
MsaG020242 AT2G25000.2 40.385 104 62 0 326 429 63 166 1.10e-20 91.7
MsaG020242 AT2G25000.4 40.385 104 62 0 326 429 63 166 1.10e-20 91.7
MsaG020242 AT4G18170.1 44.000 100 55 1 353 452 159 257 1.43e-20 93.6
MsaG020242 AT5G41570.1 45.455 99 49 2 353 451 85 178 1.71e-20 89.7
MsaG020242 AT1G64000.1 53.247 77 35 1 348 424 96 171 1.77e-20 90.5
MsaG020242 AT5G26170.1 54.167 72 32 1 352 423 99 169 1.93e-20 89.4
MsaG020242 AT2G25000.1 40.385 104 62 0 326 429 106 209 2.30e-20 92.0
MsaG020242 AT5G46350.1 54.054 74 33 1 353 426 170 242 9.58e-20 91.3
MsaG020242 AT2G30250.1 46.512 86 45 1 345 430 307 391 1.58e-19 91.7
MsaG020242 AT2G23320.1 59.322 59 24 0 367 425 241 299 1.84e-19 90.1
MsaG020242 AT5G49520.1 46.250 80 42 1 351 430 206 284 2.76e-19 90.9
MsaG020242 AT1G29280.1 50.000 70 35 0 357 426 65 134 4.21e-19 88.2
MsaG020242 AT2G30590.1 57.812 64 26 1 367 429 314 377 5.86e-19 89.7
MsaG020242 AT4G30935.1 40.000 125 71 3 334 456 299 421 6.85e-19 90.5
MsaG020242 AT5G13080.1 52.055 73 34 1 353 425 54 125 9.78e-19 83.6
MsaG020242 AT1G55600.1 48.684 76 38 1 353 428 294 368 1.54e-18 89.7
MsaG020242 AT3G58710.2 47.945 73 38 0 357 429 61 133 2.25e-18 86.3
MsaG020242 AT4G26440.1 51.316 76 36 1 353 428 359 433 2.34e-18 89.4
MsaG020242 AT3G58710.1 47.945 73 38 0 357 429 62 134 2.36e-18 86.3
MsaG020242 AT5G28650.1 60.377 53 21 0 371 423 267 319 6.34e-18 85.9
MsaG020242 AT4G24240.1 56.667 60 26 0 366 425 281 340 8.18e-18 85.9
MsaG020242 AT3G01970.1 51.389 72 34 1 352 423 51 121 1.13e-17 80.9
MsaG020242 AT3G62340.1 52.703 74 34 1 353 426 105 177 1.15e-17 84.3
MsaG020242 AT5G45050.4 28.226 248 149 8 294 529 971 1201 1.28e-17 87.8
MsaG020242 AT5G45050.1 28.226 248 149 8 294 529 1110 1340 1.42e-17 87.8
MsaG020242 AT5G45050.2 28.226 248 149 8 294 529 1082 1312 1.45e-17 87.8
MsaG020242 AT3G04670.1 57.895 57 24 0 367 423 263 319 1.51e-17 84.7
MsaG020242 AT2G24570.1 59.649 57 23 0 367 423 244 300 1.68e-17 84.3
MsaG020242 AT2G44745.1 39.286 112 63 3 315 423 92 201 1.69e-17 82.4
MsaG020242 AT2G47260.1 45.783 83 44 1 351 433 159 240 1.76e-17 84.7
MsaG020242 AT2G46130.1 46.429 84 43 2 342 424 5 87 1.98e-17 79.0
MsaG020242 AT4G01250.1 46.429 84 43 1 353 436 117 198 2.86e-17 83.6
MsaG020242 AT2G34830.1 43.011 93 46 1 348 433 190 282 3.11e-17 85.1
MsaG020242 AT5G52830.1 45.455 77 42 0 360 436 159 235 3.19e-17 84.0
MsaG020242 AT1G30650.1 43.011 93 46 1 348 433 192 284 3.29e-17 85.1
MsaG020242 AT2G34830.2 43.011 93 46 1 348 433 199 291 3.95e-17 84.7
MsaG020242 AT4G31550.1 57.895 57 24 0 367 423 247 303 4.35e-17 83.2
MsaG020242 AT4G31550.2 57.895 57 24 0 367 423 246 302 4.46e-17 83.2
MsaG020242 AT5G64810.1 45.745 94 47 2 333 423 74 166 5.40e-17 80.1
MsaG020242 AT4G23550.1 44.872 78 43 0 361 438 135 212 2.33e-16 80.9
MsaG020242 AT4G39410.1 52.113 71 33 1 353 423 210 279 5.15e-16 79.7
MsaG020242 AT1G69310.4 44.000 75 41 1 349 423 130 203 1.38e-15 78.2
MsaG020242 AT1G69310.2 44.000 75 41 1 349 423 130 203 1.38e-15 78.2
MsaG020242 AT1G69310.1 44.000 75 41 1 349 423 130 203 1.38e-15 78.2
MsaG020242 AT2G37260.2 53.846 65 29 1 361 425 262 325 2.56e-15 78.2
MsaG020242 AT2G37260.2 48.529 68 28 3 367 429 84 149 2.18e-11 66.2
MsaG020242 AT2G37260.3 53.846 65 29 1 361 425 264 327 2.63e-15 78.2
MsaG020242 AT2G37260.3 48.529 68 28 3 367 429 86 151 2.17e-11 66.2
MsaG020242 AT2G37260.1 53.846 65 29 1 361 425 344 407 3.66e-15 78.6
MsaG020242 AT2G37260.1 48.529 68 28 3 367 429 166 231 2.43e-11 66.6
MsaG020242 AT2G21900.1 42.029 69 39 1 361 429 104 171 3.00e-13 69.7
MsaG020242 AT2G46130.2 44.048 84 41 3 342 424 5 83 5.05e-13 66.2
MsaG020242 AT5G45260.1 40.625 96 51 2 347 440 1193 1284 1.22e-12 72.0
MsaG020242 AT4G31800.3 48.438 64 28 1 345 403 150 213 5.81e-12 66.6

Find 114 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGATACACCATCTTTAT+TGG 0.104474 4:-47761328 MsaT020242.1:CDS
CCTAACTGATCTTGACTTCT+AGG 0.119265 4:+47759331 None:intergenic
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4:+47757997 None:intergenic
GACAAAGCTGCAGGAATATT+TGG 0.214770 4:+47757820 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4:+47761059 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4:-47757796 MsaT020242.1:CDS
ACGTTCCTTTGTGGAGTATT+TGG 0.251996 4:+47758036 None:intergenic
TAAAGTTTAGGTCTTGTAAT+TGG 0.259816 4:+47757605 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4:-47757997 MsaT020242.1:CDS
ACTGGGGCCAAGGAAATTTA+TGG 0.276997 4:-47759662 MsaT020242.1:CDS
ACTGTTTGAGCAGTAATATT+TGG 0.280261 4:+47757865 None:intergenic
GGGAAAGAGTTGTTATTAAC+TGG 0.286280 4:+47761573 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4:-47759742 MsaT020242.1:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4:-47759502 MsaT020242.1:CDS
GATAAGGTTAATGAAGTTAA+AGG 0.322766 4:-47759622 MsaT020242.1:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4:-47759851 MsaT020242.1:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4:-47757893 MsaT020242.1:CDS
CCAAGGAAATTTATGGAACT+TGG 0.341755 4:-47759655 MsaT020242.1:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4:+47758968 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4:-47759063 MsaT020242.1:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4:+47761113 None:intergenic
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4:+47758231 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4:-47761048 MsaT020242.1:CDS
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4:-47759680 MsaT020242.1:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4:+47759012 None:intergenic
TCCCTTTCCTAGTCAATATA+AGG 0.384368 4:-47761482 MsaT020242.1:CDS
AACTGATTTACGTTCCTTTG+TGG 0.388792 4:+47758027 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4:-47759874 MsaT020242.1:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4:-47759535 MsaT020242.1:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4:-47757745 MsaT020242.1:CDS
TCTTTGGCTGCTGCAGGTAA+AGG 0.394516 4:+47758237 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4:+47758072 None:intergenic
GAAATTTATGGAACTTGGAT+TGG 0.404712 4:-47759650 MsaT020242.1:CDS
ACTTGGATTGGCTACTGATA+AGG 0.406023 4:-47759638 MsaT020242.1:CDS
TAACATCCTCATTCTTACTT+AGG 0.406214 4:+47759266 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4:+47759743 None:intergenic
GCTACCATTGCTATTTCCTT+CGG 0.410299 4:+47757711 None:intergenic
CTTGTTTCATGAGATGAGAT+TGG 0.411363 4:+47761435 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4:+47761552 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.418387 4:+47757763 None:intergenic
CTTTGGCTGCTGCAGGTAAA+GGG 0.420375 4:+47758238 None:intergenic
CCAAGTTCCATAAATTTCCT+TGG 0.423079 4:+47759655 None:intergenic
GTGTAATCAATTGTAATAGT+TGG 0.425606 4:+47758066 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4:+47758974 None:intergenic
TGATGGGAAGGAACAAAATC+AGG 0.431895 4:-47757632 MsaT020242.1:CDS
AAAATGAGAATTAAGAATGA+TGG 0.434196 4:-47759568 MsaT020242.1:CDS
GTATGAATATGATCATTGTC+AGG 0.443290 4:+47761348 None:intergenic
TGGATGAGTATCACCAATAA+TGG 0.445729 4:+47757783 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4:-47759512 MsaT020242.1:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.461509 4:-47758232 MsaT020242.1:CDS
AGCTTTAGTTCCAATAAAGA+TGG 0.463299 4:+47761318 None:intergenic
GACCTTATATTGACTAGGAA+AGG 0.470626 4:+47761480 None:intergenic
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4:-47759679 MsaT020242.1:CDS
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4:-47759331 MsaT020242.1:CDS
ACATGTGGCATCAACAATGA+TGG 0.482281 4:-47757649 MsaT020242.1:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4:-47759530 MsaT020242.1:CDS
GCATCAACATCAAGCAATGA+TGG 0.494138 4:-47758171 MsaT020242.1:CDS
GTTGGTGTCAAGGCAATGTA+AGG 0.496162 4:-47759422 MsaT020242.1:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4:-47759518 MsaT020242.1:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4:+47758983 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4:-47759552 MsaT020242.1:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4:+47758973 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4:-47759758 MsaT020242.1:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4:+47757886 None:intergenic
TCATGAAACAAGAACAGAGT+TGG 0.518840 4:-47761424 MsaT020242.1:CDS
TAGTAGTGATCAATCTATGA+TGG 0.520634 4:-47761096 MsaT020242.1:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4:+47759018 None:intergenic
ACCTTATATTGACTAGGAAA+GGG 0.537487 4:+47761481 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4:-47759084 MsaT020242.1:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4:+47761535 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.543545 4:-47758273 MsaT020242.1:CDS
ATGGTGGAGTACTGGGGCCA+AGG 0.545172 4:-47759672 MsaT020242.1:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4:+47758984 None:intergenic
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4:+47757989 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4:-47759440 MsaT020242.1:CDS
AGTATTGATAGTAATAGGAA+TGG 0.556477 4:+47761118 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4:-47759484 MsaT020242.1:CDS
GTATTGATAGTAATAGGAAT+GGG 0.563883 4:+47761119 None:intergenic
TATCGCTGCAGCACGACCCA+TGG 0.564450 4:-47759000 MsaT020242.1:CDS
ATAGCAGCTGACAAAGCTGC+AGG 0.574982 4:+47757811 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4:-47761045 MsaT020242.1:intron
GATAATAAGCTTTAGGACAC+GGG 0.575823 4:+47759019 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4:-47761089 MsaT020242.1:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4:-47759074 MsaT020242.1:CDS
AGCAACAAAATTATCACATG+TGG 0.580366 4:-47757664 MsaT020242.1:CDS
GTATTGCCAATATTTGTGAA+GGG 0.582929 4:+47757887 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4:-47757648 MsaT020242.1:CDS
TACAAATGCTGATATTGTGA+TGG 0.589604 4:-47759386 MsaT020242.1:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4:-47759272 MsaT020242.1:CDS
ATGATCATTGTCAGGGACAG+AGG 0.594495 4:+47761356 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.594834 4:+47761349 None:intergenic
ATGGTGGAGAGTTGGTGTCA+AGG 0.595504 4:-47759432 MsaT020242.1:CDS
TGGCATCAACAATGATGGGA+AGG 0.604261 4:-47757644 MsaT020242.1:CDS
ATTAACTGGAACTGAAGCCA+TGG 0.605747 4:+47761587 None:intergenic
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4:+47757969 None:intergenic
AATATTTGGACTAGCAGCAA+TGG 0.612014 4:+47757834 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4:-47759062 MsaT020242.1:CDS
TGGAGGACCTTATATTGACT+AGG 0.616940 4:+47761475 None:intergenic
TTAAAACTTAACAGGTACAA+AGG 0.618245 4:-47758314 MsaT020242.1:intron
TGTTAATGTTGATAATGTTG+AGG 0.619880 4:-47759248 MsaT020242.1:CDS
AAGTCAAGATCAGTTAGGAT+CGG 0.620379 4:-47759326 MsaT020242.1:CDS
TGGCTGCTGCAGGTAAAGGG+TGG 0.624702 4:+47758241 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4:+47761553 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.626521 4:-47757715 MsaT020242.1:CDS
AATGGCAACAATGTCACCGA+AGG 0.626879 4:-47757727 MsaT020242.1:CDS
AGCTAGATCAGAAGAAACCA+TGG 0.628836 4:-47759194 MsaT020242.1:intron
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4:+47757988 None:intergenic
TTCATGAGATGAGATTGGAG+AGG 0.650096 4:+47761440 None:intergenic
AGGCAGAGACAACTTCATGA+TGG 0.653739 4:+47761376 None:intergenic
GAATAGATTACAATTACATG+CGG 0.660089 4:-47759792 MsaT020242.1:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4:-47758041 MsaT020242.1:CDS
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4:-47759678 MsaT020242.1:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4:+47758111 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4:-47759757 MsaT020242.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 47757582 47761607 47757582 ID=MsaG020242
Chr4 mRNA 47757582 47761607 47757582 ID=MsaT020242.1;Parent=MsaG020242
Chr4 exon 47757582 47758322 47757582 ID=MsaT020242.1.exon5;Parent=MsaT020242.1
Chr4 CDS 47757582 47758322 47757582 ID=cds.MsaT020242.1;Parent=MsaT020242.1
Chr4 exon 47758981 47759094 47758981 ID=MsaT020242.1.exon4;Parent=MsaT020242.1
Chr4 CDS 47758981 47759094 47758981 ID=cds.MsaT020242.1;Parent=MsaT020242.1
Chr4 exon 47759195 47759890 47759195 ID=MsaT020242.1.exon3;Parent=MsaT020242.1
Chr4 CDS 47759195 47759890 47759195 ID=cds.MsaT020242.1;Parent=MsaT020242.1
Chr4 exon 47761046 47761147 47761046 ID=MsaT020242.1.exon2;Parent=MsaT020242.1
Chr4 CDS 47761046 47761147 47761046 ID=cds.MsaT020242.1;Parent=MsaT020242.1
Chr4 exon 47761314 47761607 47761314 ID=MsaT020242.1.exon1;Parent=MsaT020242.1
Chr4 CDS 47761314 47761607 47761314 ID=cds.MsaT020242.1;Parent=MsaT020242.1
Gene Sequence

>MsaT020242.1

ATGGCTTCAGTTCCAGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATAAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCTCATGAAACAAGAACAGAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGCCTCTGTCCCTGACAATGATCATATTCATACTGATACACCATCTTTATTGGAACTAAAGCTAAGCATTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCGGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAAGGAAATTTATGGAACTTGGATTGGCTACTGATAAGGTTAATGAAGTTAAAGGACAAAAAGTTTCTGATCAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGTCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTTGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCGGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAAAGCTAGATCAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTACAAAGGTGTGCTTTTGACAAAACAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGAAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGTAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAGTTAATGCTGCCATTGCTGCTAGTCCAAATATTCCTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCATCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAACAAAATCAGGAATACCAATTACAAGACCTAAACTTTAATTTTTTTTGCTCAAAGATTTAG
Protein sequence

>MsaT020242.1

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHSTIPFLVNIRSSNENQTSPISSHETRTELDFFDDNNNDHHEVVSASVPDNDHIHTDTPSLLELKLSIGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMRKRFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVLGPRKFMELGLATDKVNEVKGQKVSDQKFVEKMRIKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELVSRQCKDLVLITNADIVMDHEALSSSMRKPRSQDQLGSAMKNIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIKARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCAFDKTVVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSASTSSNDGQLNENLLTRTLLPCSSSIATISASAPYPTITIDYTQSPNTPQRNVNQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTVNAAIAASPNIPAALSAAITSIIGDTHPNNNTAAADNNNNGNNVTEGNSNGSNNNVTEANSNKIITCGINNDGKEQNQEYQLQDLNFNFFCSKI*