AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020268


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020268 MtrunA17_Chr4g0025361 94.697 132 2 2 1 132 51 177 7.30e-86 248
MsaG020268 MtrunA17_Chr2g0322221 65.972 144 35 6 1 132 54 195 8.13e-50 157
MsaG020268 MtrunA17_Chr4g0063931 62.245 98 31 2 34 125 84 181 5.83e-31 109
MsaG020268 MtrunA17_Chr4g0011841 92.000 50 4 0 63 112 135 184 3.55e-28 102
MsaG020268 MtrunA17_Chr4g0056521 63.291 79 20 2 63 132 130 208 1.56e-27 101
MsaG020268 MtrunA17_Chr5g0413381 49.462 93 41 2 21 107 70 162 3.24e-22 87.0
MsaG020268 MtrunA17_Chr5g0419871 69.091 55 15 1 54 106 101 155 9.19e-19 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020268 AT1G08465.1 55.147 136 48 5 5 132 54 184 2.02e-39 130
MsaG020268 AT2G45190.2 60.952 105 30 3 39 132 49 153 1.18e-33 115
MsaG020268 AT2G45190.1 61.538 104 29 3 40 132 114 217 4.29e-33 116
MsaG020268 AT2G26580.2 65.591 93 26 2 31 118 72 163 6.34e-30 106
MsaG020268 AT2G26580.1 65.591 93 26 2 31 118 72 163 6.34e-30 106
MsaG020268 AT4G00180.2 67.089 79 17 1 63 132 120 198 3.18e-29 105
MsaG020268 AT4G00180.1 67.089 79 17 1 63 132 151 229 7.02e-29 105
MsaG020268 AT1G23420.1 70.588 51 15 0 60 110 130 180 1.48e-18 78.6
MsaG020268 AT1G69180.1 66.667 48 16 0 59 106 109 156 1.75e-18 77.4
MsaG020268 AT1G23420.2 70.588 51 15 0 60 110 161 211 4.06e-18 77.8

Find 36 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGCTGATATCTGGATTAC+TGG 0.287474 4:-48393840 None:intergenic
GCCTCCCTGTGGCTGATATC+TGG 0.289400 4:-48393848 None:intergenic
GGAGGAAATTCAAAGGATTA+AGG 0.360502 4:+48393817 MsaT020268.1:CDS
CATTTCCCTCATATTCACTT+TGG 0.399992 4:+48395104 MsaT020268.1:CDS
GAAGCAAGCAGCAAGGAAAC+TGG 0.400281 4:+48391983 MsaT020268.1:CDS
ATTTCCCTCATATTCACTTT+GGG 0.416154 4:+48395105 MsaT020268.1:CDS
AAATAACTTACTTAATGAAC+CGG 0.422652 4:-48393391 None:intergenic
GGGAGTTGAGGAGGAGGAAA+TGG 0.440779 4:-48389741 None:intergenic
TGCTTCCCAAAGTGAATATG+AGG 0.444364 4:-48395110 None:intergenic
GGGACTCGTGAAAAGACTAA+TGG 0.445379 4:+48395158 MsaT020268.1:CDS
CGACGAATGGGAGGAATCCT+AGG 0.445790 4:-48392067 None:intergenic
GTGTTCCTTCTGCTTACAAC+CGG 0.449198 4:+48393372 MsaT020268.1:CDS
TGGGAAGCAGCAAGCAAAGT+TGG 0.453770 4:+48395124 MsaT020268.1:CDS
TCCTCCTCCTCAACTCCCTC+AGG 0.474865 4:+48389746 MsaT020268.1:CDS
CAACAAAATAGCATCATTTG+AGG 0.493091 4:+48392024 MsaT020268.1:CDS
AGAGTGTGACTCACGACGAA+TGG 0.516345 4:-48392080 None:intergenic
TAGTAGAGCACGAACATCCT+AGG 0.516780 4:+48392050 MsaT020268.1:CDS
GAGTGTGACTCACGACGAAT+GGG 0.531160 4:-48392079 None:intergenic
ACCTGAGGGAGTTGAGGAGG+AGG 0.539948 4:-48389747 None:intergenic
TGAATTATGTGCTGTTTCTG+TGG 0.544740 4:-48391956 None:intergenic
GGAGGAAATGGCTGAAGTAA+TGG 0.547935 4:-48389729 None:intergenic
CAGCAAGCAAAGTTGGATCA+TGG 0.562735 4:+48395131 MsaT020268.1:CDS
GCAAAGTTGGATCATGGTGA+AGG 0.573037 4:+48395137 MsaT020268.1:CDS
AAACATACCTGAGGGAGTTG+AGG 0.577695 4:-48389753 None:intergenic
ATGAACCGGTTGTAAGCAGA+AGG 0.582569 4:-48393377 None:intergenic
GTAATCCAGATATCAGCCAC+AGG 0.588300 4:+48393843 MsaT020268.1:CDS
TTTGTAGGGAGGAAATTCAA+AGG 0.595432 4:+48393810 MsaT020268.1:intron
CAAAGTTGGATCATGGTGAA+GGG 0.606710 4:+48395138 MsaT020268.1:CDS
CTGAGCTGAAAGCCTCCCTG+TGG 0.623676 4:-48393859 None:intergenic
CAGGGAGGCTTTCAGCTCAG+CGG 0.634037 4:+48393862 MsaT020268.1:CDS
CATACCTGAGGGAGTTGAGG+AGG 0.635233 4:-48389750 None:intergenic
TGTGACTCACGACGAATGGG+AGG 0.645995 4:-48392076 None:intergenic
TCATGAAGAAGCAAGCAGCA+AGG 0.659976 4:+48391976 MsaT020268.1:CDS
GCTTCCCAAAGTGAATATGA+GGG 0.661736 4:-48395109 None:intergenic
TCCAGATATCAGCCACAGGG+AGG 0.683704 4:+48393847 MsaT020268.1:CDS
TAATCCAGATATCAGCCACA+GGG 0.696359 4:+48393844 MsaT020268.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 48389720 48395190 48389720 ID=MsaG020268
Chr4 mRNA 48389720 48395190 48389720 ID=MsaT020268.1;Parent=MsaG020268
Chr4 exon 48389720 48389767 48389720 ID=MsaT020268.1.exon1;Parent=MsaT020268.1
Chr4 CDS 48389720 48389767 48389720 ID=cds.MsaT020268.1;Parent=MsaT020268.1
Chr4 exon 48391956 48392088 48391956 ID=MsaT020268.1.exon2;Parent=MsaT020268.1
Chr4 CDS 48391956 48392088 48391956 ID=cds.MsaT020268.1;Parent=MsaT020268.1
Chr4 exon 48393354 48393402 48393354 ID=MsaT020268.1.exon3;Parent=MsaT020268.1
Chr4 CDS 48393354 48393402 48393354 ID=cds.MsaT020268.1;Parent=MsaT020268.1
Chr4 exon 48393817 48393892 48393817 ID=MsaT020268.1.exon4;Parent=MsaT020268.1
Chr4 CDS 48393817 48393892 48393817 ID=cds.MsaT020268.1;Parent=MsaT020268.1
Chr4 exon 48395098 48395190 48395098 ID=MsaT020268.1.exon5;Parent=MsaT020268.1
Chr4 CDS 48395098 48395190 48395098 ID=cds.MsaT020268.1;Parent=MsaT020268.1
Gene Sequence

>MsaT020268.1

ATGGTGGCACCATTACTTCAGCCATTTCCTCCTCCTCAACTCCCTCAGCCACAGAAACAGCACATAATTCATGAAGAAGCAAGCAGCAAGGAAACTGGATCATCATCTTCAAAATGCAACAAAATAGCATCATTTGAGGCAGTAGTAGAGCACGAACATCCTAGGATTCCTCCCATTCGTCCTATAGAGAAAAGACATCGTGTTCCTTCTGCTTACAACCGGTTCATTAAGGAGGAAATTCAAAGGATTAAGGCCAGTAATCCAGATATCAGCCACAGGGAGGCTTTCAGCTCAGCGGCAAAAAATTGGGCACATTTCCCTCATATTCACTTTGGGAAGCAGCAAGCAAAGTTGGATCATGGTGAAGGGACTCGTGAAAAGACTAATGGATTCTACTGA

Protein sequence

>MsaT020268.1

MVAPLLQPFPPPQLPQPQKQHIIHEEASSKETGSSSSKCNKIASFEAVVEHEHPRIPPIRPIEKRHRVPSAYNRFIKEEIQRIKASNPDISHREAFSSAAKNWAHFPHIHFGKQQAKLDHGEGTREKTNGFY*