Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG020268 | XP_003605973.1 | 96.212 | 132 | 2 | 1 | 1 | 132 | 51 | 179 | 2.65e-85 | 256 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG020268 | sp|Q9XFB0|YAB2_ARATH | 55.147 | 136 | 48 | 5 | 5 | 132 | 54 | 184 | 1.99e-38 | 130 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG020268 | tr|G7JER5|G7JER5_MEDTR | 96.212 | 132 | 2 | 1 | 1 | 132 | 51 | 179 | 1.27e-85 | 256 |
Gene ID | Type | Classification |
---|---|---|
MsaG020268 | TF | C2C2-YABBY |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000242 | MsaG020268 | 0.835075 | 3.775834e-56 | 1.267628e-53 |
MsaG000250 | MsaG020268 | 0.852792 | 7.022867e-61 | 4.194376e-58 |
MsaG000722 | MsaG020268 | 0.804294 | 3.904864e-49 | 5.824089e-47 |
MsaG000998 | MsaG020268 | 0.805530 | 2.159532e-49 | 3.314514e-47 |
MsaG001878 | MsaG020268 | 0.805184 | 2.550722e-49 | 3.883638e-47 |
MsaG003536 | MsaG020268 | 0.801829 | 1.255438e-48 | 1.769172e-46 |
MsaG003679 | MsaG020268 | 0.807093 | 1.015443e-49 | 1.617031e-47 |
MsaG003998 | MsaG020268 | 0.819043 | 2.495598e-52 | 5.352261e-50 |
MsaG004656 | MsaG020268 | 0.836014 | 2.190285e-56 | 7.563104e-54 |
MsaG006240 | MsaG020268 | 0.801128 | 1.744945e-48 | 2.420122e-46 |
MsaG006741 | MsaG020268 | 0.826172 | 5.584678e-54 | 1.451176e-51 |
MsaG007240 | MsaG020268 | 0.806112 | 1.632077e-49 | 2.539495e-47 |
MsaG008088 | MsaG020268 | 0.803061 | 7.018278e-49 | 1.017278e-46 |
MsaG009067 | MsaG020268 | 0.810708 | 1.725214e-50 | 2.997448e-48 |
MsaG009261 | MsaG020268 | 0.806605 | 1.286002e-49 | 2.024573e-47 |
MsaG010221 | MsaG020268 | 0.813446 | 4.392780e-51 | 8.166705e-49 |
MsaG011861 | MsaG020268 | 0.820577 | 1.117649e-52 | 2.495927e-50 |
MsaG012446 | MsaG020268 | 0.806498 | 1.354534e-49 | 2.127053e-47 |
MsaG016273 | MsaG020268 | 0.802243 | 1.033400e-48 | 1.469991e-46 |
MsaG018848 | MsaG020268 | 0.811182 | 1.363829e-50 | 2.397207e-48 |
MsaG019091 | MsaG020268 | 0.809058 | 3.893758e-50 | 6.500096e-48 |
MsaG019092 | MsaG020268 | 0.800296 | 2.575596e-48 | 3.505610e-46 |
MsaG019132 | MsaG020268 | 0.803854 | 4.815357e-49 | 7.108809e-47 |
MsaG019795 | MsaG020268 | 0.831327 | 3.205411e-55 | 9.636820e-53 |
MsaG019814 | MsaG020268 | 0.822901 | 3.261162e-53 | 7.748568e-51 |
MsaG020268 | MsaG021283 | 0.800182 | 2.715911e-48 | 3.687079e-46 |
MsaG020268 | MsaG021564 | 0.821748 | 6.022156e-53 | 1.387243e-50 |
MsaG020268 | MsaG023960 | 0.830544 | 4.977840e-55 | 1.463148e-52 |
MsaG020268 | MsaG024139 | 0.805645 | 2.043317e-49 | 3.144693e-47 |
MsaG020268 | MsaG025644 | 0.810707 | 1.726233e-50 | 2.999139e-48 |
MsaG020268 | MsaG025732 | 0.802798 | 7.947832e-49 | 1.145046e-46 |
MsaG020268 | MsaG027241 | 0.802587 | 8.781362e-49 | 1.259092e-46 |
MsaG020268 | MsaG028248 | 0.807277 | 9.286618e-50 | 1.485289e-47 |
MsaG020268 | MsaG029694 | 0.823675 | 2.155105e-53 | 5.229222e-51 |
MsaG020268 | MsaG030047 | 0.805137 | 2.608875e-49 | 3.967873e-47 |
MsaG020268 | MsaG031201 | 0.813883 | 3.524674e-51 | 6.624980e-49 |
MsaG020268 | MsaG034133 | 0.824525 | 1.363781e-53 | 3.386480e-51 |
MsaG020268 | MsaG035513 | 0.822717 | 3.597727e-53 | 8.505884e-51 |
MsaG020268 | MsaG039781 | 0.800694 | 2.138594e-48 | 2.937272e-46 |
MsaG020268 | MsaG042071 | 0.813517 | 4.238542e-51 | 7.894050e-49 |
MsaG020268 | MsaG042791 | 0.804348 | 3.804910e-49 | 5.682023e-47 |
MsaG020268 | MsaG044092 | 0.807310 | 9.137932e-50 | 1.462671e-47 |
MsaG020268 | MsaG044107 | 0.813409 | 4.476482e-51 | 8.314419e-49 |
MsaG020268 | MsaG044516 | 0.812818 | 6.024484e-51 | 1.102531e-48 |
MsaG020268 | MsaG045703 | 0.834696 | 4.698955e-56 | 1.559767e-53 |
MsaG020268 | MsaG046436 | 0.804781 | 3.093071e-49 | 4.665514e-47 |
MsaG020268 | MsaG046497 | 0.819293 | 2.191231e-52 | 4.730416e-50 |
MsaG020268 | MsaG046926 | 0.818425 | 3.443107e-52 | 7.265668e-50 |
MsaG020268 | MsaG004075 | 0.803355 | 6.104857e-49 | 8.909226e-47 |
MsaG020268 | MsaG007986 | 0.847899 | 1.632682e-59 | 8.240479e-57 |
MsaG020268 | MsaG008901 | 0.851261 | 1.902853e-60 | 1.077107e-57 |
MsaG020268 | MsaG012932 | 0.816935 | 7.436415e-52 | 1.510168e-49 |
MsaG020268 | MsaG027166 | 0.802440 | 9.415643e-49 | 1.345463e-46 |
MsaG020268 | MsaG030688 | 0.816350 | 1.004155e-51 | 2.008793e-49 |
MsaG020268 | MsaG042416 | 0.802331 | 9.914128e-49 | 1.413193e-46 |
MsaG020268 | MsaG040992 | 0.805681 | 2.008438e-49 | 3.093558e-47 |
MsaG020268 | MsaG041446 | 0.807850 | 7.028859e-50 | 1.139740e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG020268 | MtrunA17_Chr4g0025361 | 94.697 | 132 | 2 | 2 | 1 | 132 | 51 | 177 | 7.30e-86 | 248 |
MsaG020268 | MtrunA17_Chr2g0322221 | 65.972 | 144 | 35 | 6 | 1 | 132 | 54 | 195 | 8.13e-50 | 157 |
MsaG020268 | MtrunA17_Chr4g0063931 | 62.245 | 98 | 31 | 2 | 34 | 125 | 84 | 181 | 5.83e-31 | 109 |
MsaG020268 | MtrunA17_Chr4g0011841 | 92.000 | 50 | 4 | 0 | 63 | 112 | 135 | 184 | 3.55e-28 | 102 |
MsaG020268 | MtrunA17_Chr4g0056521 | 63.291 | 79 | 20 | 2 | 63 | 132 | 130 | 208 | 1.56e-27 | 101 |
MsaG020268 | MtrunA17_Chr5g0413381 | 49.462 | 93 | 41 | 2 | 21 | 107 | 70 | 162 | 3.24e-22 | 87.0 |
MsaG020268 | MtrunA17_Chr5g0419871 | 69.091 | 55 | 15 | 1 | 54 | 106 | 101 | 155 | 9.19e-19 | 77.4 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG020268 | AT1G08465.1 | 55.147 | 136 | 48 | 5 | 5 | 132 | 54 | 184 | 2.02e-39 | 130 |
MsaG020268 | AT2G45190.2 | 60.952 | 105 | 30 | 3 | 39 | 132 | 49 | 153 | 1.18e-33 | 115 |
MsaG020268 | AT2G45190.1 | 61.538 | 104 | 29 | 3 | 40 | 132 | 114 | 217 | 4.29e-33 | 116 |
MsaG020268 | AT2G26580.2 | 65.591 | 93 | 26 | 2 | 31 | 118 | 72 | 163 | 6.34e-30 | 106 |
MsaG020268 | AT2G26580.1 | 65.591 | 93 | 26 | 2 | 31 | 118 | 72 | 163 | 6.34e-30 | 106 |
MsaG020268 | AT4G00180.2 | 67.089 | 79 | 17 | 1 | 63 | 132 | 120 | 198 | 3.18e-29 | 105 |
MsaG020268 | AT4G00180.1 | 67.089 | 79 | 17 | 1 | 63 | 132 | 151 | 229 | 7.02e-29 | 105 |
MsaG020268 | AT1G23420.1 | 70.588 | 51 | 15 | 0 | 60 | 110 | 130 | 180 | 1.48e-18 | 78.6 |
MsaG020268 | AT1G69180.1 | 66.667 | 48 | 16 | 0 | 59 | 106 | 109 | 156 | 1.75e-18 | 77.4 |
MsaG020268 | AT1G23420.2 | 70.588 | 51 | 15 | 0 | 60 | 110 | 161 | 211 | 4.06e-18 | 77.8 |
Find 36 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GTGGCTGATATCTGGATTAC+TGG | 0.287474 | 4:-48393840 | None:intergenic |
GCCTCCCTGTGGCTGATATC+TGG | 0.289400 | 4:-48393848 | None:intergenic |
GGAGGAAATTCAAAGGATTA+AGG | 0.360502 | 4:+48393817 | MsaT020268.1:CDS |
CATTTCCCTCATATTCACTT+TGG | 0.399992 | 4:+48395104 | MsaT020268.1:CDS |
GAAGCAAGCAGCAAGGAAAC+TGG | 0.400281 | 4:+48391983 | MsaT020268.1:CDS |
ATTTCCCTCATATTCACTTT+GGG | 0.416154 | 4:+48395105 | MsaT020268.1:CDS |
AAATAACTTACTTAATGAAC+CGG | 0.422652 | 4:-48393391 | None:intergenic |
GGGAGTTGAGGAGGAGGAAA+TGG | 0.440779 | 4:-48389741 | None:intergenic |
TGCTTCCCAAAGTGAATATG+AGG | 0.444364 | 4:-48395110 | None:intergenic |
GGGACTCGTGAAAAGACTAA+TGG | 0.445379 | 4:+48395158 | MsaT020268.1:CDS |
CGACGAATGGGAGGAATCCT+AGG | 0.445790 | 4:-48392067 | None:intergenic |
GTGTTCCTTCTGCTTACAAC+CGG | 0.449198 | 4:+48393372 | MsaT020268.1:CDS |
TGGGAAGCAGCAAGCAAAGT+TGG | 0.453770 | 4:+48395124 | MsaT020268.1:CDS |
TCCTCCTCCTCAACTCCCTC+AGG | 0.474865 | 4:+48389746 | MsaT020268.1:CDS |
CAACAAAATAGCATCATTTG+AGG | 0.493091 | 4:+48392024 | MsaT020268.1:CDS |
AGAGTGTGACTCACGACGAA+TGG | 0.516345 | 4:-48392080 | None:intergenic |
TAGTAGAGCACGAACATCCT+AGG | 0.516780 | 4:+48392050 | MsaT020268.1:CDS |
GAGTGTGACTCACGACGAAT+GGG | 0.531160 | 4:-48392079 | None:intergenic |
ACCTGAGGGAGTTGAGGAGG+AGG | 0.539948 | 4:-48389747 | None:intergenic |
TGAATTATGTGCTGTTTCTG+TGG | 0.544740 | 4:-48391956 | None:intergenic |
GGAGGAAATGGCTGAAGTAA+TGG | 0.547935 | 4:-48389729 | None:intergenic |
CAGCAAGCAAAGTTGGATCA+TGG | 0.562735 | 4:+48395131 | MsaT020268.1:CDS |
GCAAAGTTGGATCATGGTGA+AGG | 0.573037 | 4:+48395137 | MsaT020268.1:CDS |
AAACATACCTGAGGGAGTTG+AGG | 0.577695 | 4:-48389753 | None:intergenic |
ATGAACCGGTTGTAAGCAGA+AGG | 0.582569 | 4:-48393377 | None:intergenic |
GTAATCCAGATATCAGCCAC+AGG | 0.588300 | 4:+48393843 | MsaT020268.1:CDS |
TTTGTAGGGAGGAAATTCAA+AGG | 0.595432 | 4:+48393810 | MsaT020268.1:intron |
CAAAGTTGGATCATGGTGAA+GGG | 0.606710 | 4:+48395138 | MsaT020268.1:CDS |
CTGAGCTGAAAGCCTCCCTG+TGG | 0.623676 | 4:-48393859 | None:intergenic |
CAGGGAGGCTTTCAGCTCAG+CGG | 0.634037 | 4:+48393862 | MsaT020268.1:CDS |
CATACCTGAGGGAGTTGAGG+AGG | 0.635233 | 4:-48389750 | None:intergenic |
TGTGACTCACGACGAATGGG+AGG | 0.645995 | 4:-48392076 | None:intergenic |
TCATGAAGAAGCAAGCAGCA+AGG | 0.659976 | 4:+48391976 | MsaT020268.1:CDS |
GCTTCCCAAAGTGAATATGA+GGG | 0.661736 | 4:-48395109 | None:intergenic |
TCCAGATATCAGCCACAGGG+AGG | 0.683704 | 4:+48393847 | MsaT020268.1:CDS |
TAATCCAGATATCAGCCACA+GGG | 0.696359 | 4:+48393844 | MsaT020268.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr4 | gene | 48389720 | 48395190 | 48389720 | ID=MsaG020268 |
Chr4 | mRNA | 48389720 | 48395190 | 48389720 | ID=MsaT020268.1;Parent=MsaG020268 |
Chr4 | exon | 48389720 | 48389767 | 48389720 | ID=MsaT020268.1.exon1;Parent=MsaT020268.1 |
Chr4 | CDS | 48389720 | 48389767 | 48389720 | ID=cds.MsaT020268.1;Parent=MsaT020268.1 |
Chr4 | exon | 48391956 | 48392088 | 48391956 | ID=MsaT020268.1.exon2;Parent=MsaT020268.1 |
Chr4 | CDS | 48391956 | 48392088 | 48391956 | ID=cds.MsaT020268.1;Parent=MsaT020268.1 |
Chr4 | exon | 48393354 | 48393402 | 48393354 | ID=MsaT020268.1.exon3;Parent=MsaT020268.1 |
Chr4 | CDS | 48393354 | 48393402 | 48393354 | ID=cds.MsaT020268.1;Parent=MsaT020268.1 |
Chr4 | exon | 48393817 | 48393892 | 48393817 | ID=MsaT020268.1.exon4;Parent=MsaT020268.1 |
Chr4 | CDS | 48393817 | 48393892 | 48393817 | ID=cds.MsaT020268.1;Parent=MsaT020268.1 |
Chr4 | exon | 48395098 | 48395190 | 48395098 | ID=MsaT020268.1.exon5;Parent=MsaT020268.1 |
Chr4 | CDS | 48395098 | 48395190 | 48395098 | ID=cds.MsaT020268.1;Parent=MsaT020268.1 |
Gene Sequence |
Protein sequence |