AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020293

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020293 MtrunA17_Chr4g0025521 95.499 711 31 1 1 710 1 711 0.0 1321
MsaG020293 MtrunA17_Chr7g0267481 44.939 741 296 23 1 710 1 660 2.52e-165 491
MsaG020293 MtrunA17_Chr1g0177751 46.395 735 283 28 1 710 1 649 4.03e-164 487
MsaG020293 MtrunA17_Chr1g0155681 58.052 267 97 4 218 476 172 431 4.14e-95 307
MsaG020293 MtrunA17_Chr3g0141941 52.412 311 124 8 188 492 143 435 5.58e-95 303
MsaG020293 MtrunA17_Chr8g0373801 50.890 281 131 3 216 490 388 667 2.41e-83 281
MsaG020293 MtrunA17_Chr5g0403251 51.273 275 126 4 206 476 329 599 3.55e-78 265
MsaG020293 MtrunA17_Chr8g0387321 52.708 277 118 3 216 486 185 454 4.22e-78 262
MsaG020293 MtrunA17_Chr3g0141931 45.172 290 141 4 209 490 158 437 1.03e-71 240
MsaG020293 MtrunA17_Chr4g0028701 39.506 324 144 5 216 493 245 562 9.52e-64 224
MsaG020293 MtrunA17_Chr4g0008591 42.908 282 124 4 216 489 196 448 5.61e-62 218
MsaG020293 MtrunA17_Chr7g0240371 43.590 273 133 6 216 484 137 392 4.40e-60 211
MsaG020293 MtrunA17_Chr2g0327021 38.854 314 163 7 216 508 248 553 5.85e-59 210
MsaG020293 MtrunA17_Chr1g0151681 36.667 330 169 9 179 480 186 503 2.70e-52 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020293 AT2G35940.3 42.781 755 312 27 1 710 1 680 3.38e-133 409
MsaG020293 AT2G35940.1 42.781 755 312 27 1 710 1 680 3.38e-133 409
MsaG020293 AT2G35940.2 42.781 755 312 27 1 710 1 680 3.38e-133 409
MsaG020293 AT2G16400.1 49.077 379 152 10 149 522 57 399 2.48e-97 309
MsaG020293 AT4G34610.2 50.568 352 143 10 175 516 94 424 1.36e-95 306
MsaG020293 AT4G34610.4 50.568 352 143 10 175 516 94 424 1.36e-95 306
MsaG020293 AT4G34610.3 50.568 352 143 10 175 516 94 424 1.36e-95 306
MsaG020293 AT4G34610.1 50.568 352 143 10 175 516 94 424 1.36e-95 306
MsaG020293 AT1G75410.1 53.310 287 111 7 205 482 160 432 3.64e-88 286
MsaG020293 AT1G75410.2 53.310 287 111 7 205 482 160 432 3.64e-88 286
MsaG020293 AT1G19700.1 53.069 277 99 6 217 477 166 427 1.85e-87 285
MsaG020293 AT1G19700.3 53.069 277 99 6 217 477 166 427 1.85e-87 285
MsaG020293 AT1G19700.5 53.069 277 99 6 217 477 166 427 1.85e-87 285
MsaG020293 AT1G19700.4 53.069 277 99 6 217 477 166 427 1.85e-87 285
MsaG020293 AT1G19700.2 53.069 277 99 6 217 477 166 427 1.85e-87 285
MsaG020293 AT2G27220.1 46.648 358 144 9 146 501 12 324 3.92e-86 278
MsaG020293 AT2G27220.3 46.648 358 144 9 146 501 12 324 3.92e-86 278
MsaG020293 AT2G27220.2 44.415 376 144 10 146 501 12 342 5.31e-82 268
MsaG020293 AT2G23760.2 52.809 267 112 3 208 468 233 491 2.11e-80 269
MsaG020293 AT2G23760.1 52.809 267 112 3 208 468 233 491 2.11e-80 269
MsaG020293 AT2G23760.3 52.809 267 112 3 208 468 233 491 2.11e-80 269
MsaG020293 AT2G23760.4 44.201 319 112 4 208 468 233 543 1.40e-70 243
MsaG020293 AT1G75410.3 50.973 257 90 7 205 448 160 393 1.66e-70 236
MsaG020293 AT5G41410.1 46.014 276 130 3 217 476 194 466 1.56e-68 236
MsaG020293 AT4G36870.4 47.500 240 115 3 216 449 312 546 6.42e-63 219
MsaG020293 AT4G36870.3 47.059 238 115 3 216 447 312 544 9.42e-62 217
MsaG020293 AT1G75430.1 40.484 289 146 8 210 481 10 289 2.17e-60 205
MsaG020293 AT5G02030.1 41.137 299 123 4 197 472 151 419 1.13e-59 211
MsaG020293 AT4G32980.1 37.460 315 165 7 177 478 154 449 1.29e-51 186
MsaG020293 AT4G32980.2 37.460 315 165 7 177 478 154 449 1.29e-51 186
MsaG020293 AT2G27990.1 38.562 306 152 8 174 473 221 496 2.14e-49 183
MsaG020293 AT1G75430.2 35.526 228 128 7 210 427 10 228 7.67e-32 125
MsaG020293 AT2G27990.2 31.154 260 143 8 174 427 221 450 2.28e-23 104

Find 183 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCCACCTTCCACCTTTCA+TGG 0.242874 4:+48936533 MsaT020293.1:CDS
TGATGTTGACATTGAATTAT+TGG 0.252974 4:-48940919 None:intergenic
CTCTCTCAGAATTTACATTT+AGG 0.274546 4:+48941317 MsaT020293.1:CDS
GGGTCCTTTCAATCCAATTC+TGG 0.288442 4:+48940960 MsaT020293.1:CDS
CTGCTTTGAGATTGTCTTCA+AGG 0.297342 4:-48938868 None:intergenic
AATTCTGGTTTCCATCTTGC+AGG 0.297418 4:+48940975 MsaT020293.1:CDS
GTGTTAAGTTAGAAGGTTCT+AGG 0.298713 4:+48938967 MsaT020293.1:CDS
TCTTGCAAGCATAACCTTAT+CGG 0.300640 4:-48939911 None:intergenic
ACAAATAGATCAAAAGAATT+AGG 0.307786 4:+48940807 MsaT020293.1:CDS
TCACTGTTGGAGAAAGGTTT+TGG 0.308937 4:-48940887 None:intergenic
CTTCGCAACAAACGCCTTAC+CGG 0.309937 4:+48936819 MsaT020293.1:CDS
AGGAGGGACGATTGATGAAA+TGG 0.313418 4:+48941337 MsaT020293.1:CDS
TTTGAGCAAGCAACAGGATA+TGG 0.320173 4:+48938819 MsaT020293.1:CDS
TGCTTGGGTGTTAAGTTAGA+AGG 0.330959 4:+48938960 MsaT020293.1:CDS
TCAGAGTAAGGTACGTAGTT+TGG 0.340254 4:-48936365 None:intergenic
GGTTCTCCCCACGCGCTTCA+TGG 0.344045 4:+48936452 MsaT020293.1:CDS
TCAAGGTAGTTTCCTTTCAA+TGG 0.367613 4:+48941034 MsaT020293.1:CDS
GAGTTTCGCCCGCAGCAAAT+TGG 0.368022 4:+48936752 MsaT020293.1:CDS
GACAAACCACGCTGATAAAC+TGG 0.368350 4:-48936785 None:intergenic
AATCATCATGGTGGTGGTTA+TGG 0.369811 4:+48941155 MsaT020293.1:CDS
GTTATGCTTGCAAGACAAAC+TGG 0.371730 4:+48939919 MsaT020293.1:CDS
TGATCTATTTGTGTTATCCT+TGG 0.372128 4:-48940796 None:intergenic
AGAACAGAAACAGCTCTTTC+AGG 0.372428 4:-48939083 None:intergenic
GGAGGGACGATTGATGAAAT+GGG 0.373488 4:+48941338 MsaT020293.1:CDS
CACTATAACATGTGGGGATC+AGG 0.378783 4:+48936644 MsaT020293.1:CDS
CAAGACAAACTGGACTTACT+AGG 0.381649 4:+48939929 MsaT020293.1:CDS
TCTCAATGGAAGATGTTAGA+AGG 0.381842 4:+48941183 MsaT020293.1:CDS
ATATCACACTTTGCATTTGA+TGG 0.388198 4:-48938791 None:intergenic
CTGTTTCTGTTCTTCGTGCT+TGG 0.388531 4:+48939093 MsaT020293.1:CDS
AACGCTACCAATAGCAATAA+TGG 0.388746 4:+48936431 MsaT020293.1:CDS
TTTCATTTCCACTCAATAAA+GGG 0.390624 4:-48941133 None:intergenic
GAAATCCAACCGTCGGTAGA+AGG 0.392044 4:+48936317 MsaT020293.1:CDS
ATTATTCTCCTCTTGCTCCT+TGG 0.402178 4:-48940769 None:intergenic
GTTGCTGTGACAGCGTTATC+AGG 0.403199 4:-48936677 None:intergenic
TAAGACAGCTAGCAAGACTT+TGG 0.403443 4:+48938926 MsaT020293.1:CDS
CATAACCTTATCGGAGTCCT+TGG 0.408384 4:-48939902 None:intergenic
GGGAGAACCATTATTGCTAT+TGG 0.410019 4:-48936438 None:intergenic
CTGATCCCCACATGTTATAG+TGG 0.410131 4:-48936643 None:intergenic
TGCAAGATGGAAACCAGAAT+TGG 0.412205 4:-48940973 None:intergenic
GATATGGCAACGTACTTTCA+TGG 0.416361 4:+48936272 None:intergenic
GCTGCAGTGCCCGCTGTTGT+CGG 0.418316 4:-48939013 None:intergenic
ATTTGCTGCGGGCGAAACTC+TGG 0.420271 4:-48936749 None:intergenic
AGTTAGAAGGTTCTAGGCTT+CGG 0.429264 4:+48938973 MsaT020293.1:CDS
TTGAAAGGAAACTACCTTGA+TGG 0.429916 4:-48941031 None:intergenic
AACACTACTCCTTCTCATTC+AGG 0.436567 4:+48941389 MsaT020293.1:CDS
AATGGTGTTTCTCTAACTCT+TGG 0.436577 4:+48941245 MsaT020293.1:CDS
TTGATATTGAAAACAGAAAG+AGG 0.441083 4:+48941429 MsaT020293.1:CDS
ACGCCTTACCGGTCGGCCTC+TGG 0.447088 4:+48936830 MsaT020293.1:CDS
TGGTGAGATTGAGGTATCTC+CGG 0.447805 4:+48936850 MsaT020293.1:CDS
TCATCATTTGAGCAAGCAAC+AGG 0.451763 4:+48938813 MsaT020293.1:CDS
CTCAATCAACACCACTTGCT+TGG 0.452628 4:+48936503 MsaT020293.1:CDS
TTGAGATTGTCTTCAAGGCT+AGG 0.454466 4:-48938863 None:intergenic
GCTCCTAGGTTCCATGGAAA+TGG 0.455886 4:+48941227 MsaT020293.1:CDS
TTGAAATCACTGTTGGAGAA+AGG 0.457475 4:-48940893 None:intergenic
AAGACAGCTAGCAAGACTTT+GGG 0.459364 4:+48938927 MsaT020293.1:CDS
TTGGGTGAAGATGATTGCTT+GGG 0.462431 4:+48938945 MsaT020293.1:CDS
TTGGAACTTGAAATCACTGT+TGG 0.463404 4:-48940900 None:intergenic
CAGCGGGCACTGCAGCAACT+TGG 0.465763 4:+48939020 MsaT020293.1:CDS
TTAGTGAGCAATTAGCTCCT+AGG 0.468409 4:+48941213 MsaT020293.1:CDS
GTACGTAGTTTGGATTCATA+AGG 0.468791 4:-48936355 None:intergenic
TGAAGTTGTGAATGTGGATA+AGG 0.475584 4:+48936967 MsaT020293.1:CDS
TCTCAGAATTTACATTTAGG+AGG 0.475857 4:+48941320 MsaT020293.1:CDS
GGTAGCCGTGGAGGCGAGGA+AGG 0.483983 4:+48936872 MsaT020293.1:CDS
CCACCAACTCCGACAACAGC+GGG 0.492092 4:+48939004 MsaT020293.1:CDS
CTGAGAGAGAAATCCATGTT+GGG 0.492621 4:-48941303 None:intergenic
GTGCCCGCTGTTGTCGGAGT+TGG 0.494381 4:-48939007 None:intergenic
ATTATTGCTATTGGTAGCGT+TGG 0.494646 4:-48936429 None:intergenic
GAATGATTCATAGCAATGCT+TGG 0.495045 4:+48939042 MsaT020293.1:CDS
AACTGGACTTACTAGGAGTC+AGG 0.495725 4:+48939936 MsaT020293.1:CDS
TTATTGCTATTGGTAGCGTT+GGG 0.499259 4:-48936428 None:intergenic
CAATTAGCTCCTAGGTTCCA+TGG 0.502313 4:+48941221 MsaT020293.1:CDS
TCACCAGAGGCCGACCGGTA+AGG 0.503130 4:-48936833 None:intergenic
AAGTACATTTCTTCAACCAT+TGG 0.504400 4:-48940738 None:intergenic
AATGAATACCCTTTATTGAG+TGG 0.507581 4:+48941125 MsaT020293.1:CDS
TGAGATTGTCTTCAAGGCTA+GGG 0.509850 4:-48938862 None:intergenic
GAAGTTGTGAATGTGGATAA+GGG 0.514101 4:+48936968 MsaT020293.1:CDS
TGGTTTCCACAAGCGAACTC+TGG 0.515046 4:-48940718 None:intergenic
TAGTTTCCTTTCAATGGACA+TGG 0.518524 4:+48941040 MsaT020293.1:CDS
CCACAGAGTTTGAAGTTGAA+AGG 0.523168 4:+48941087 MsaT020293.1:CDS
TCAATTCCATGTCCATTGAA+AGG 0.523266 4:-48941046 None:intergenic
CTCAGAATTTACATTTAGGA+GGG 0.524512 4:+48941321 MsaT020293.1:CDS
TTCAATGTCAACATCACCAA+TGG 0.525243 4:+48940926 MsaT020293.1:CDS
CCTCTCTCTTCAACCCAACA+TGG 0.526082 4:+48941290 MsaT020293.1:CDS
TGTAGGAGTGTCGGTAGTGT+TGG 0.526282 4:-48936558 None:intergenic
GAAACCAGAATTGGATTGAA+AGG 0.527024 4:-48940964 None:intergenic
ACGGTAGCCGAGGAGGAAAA+CGG 0.527352 4:-48936608 None:intergenic
CCCGCTGTTGTCGGAGTTGG+TGG 0.528625 4:-48939004 None:intergenic
GGTGAGATTGAGGTATCTCC+GGG 0.529905 4:+48936851 MsaT020293.1:CDS
TCCGGGTAGCCGTGGAGGCG+AGG 0.533224 4:+48936868 MsaT020293.1:CDS
TTTGGGTGAAGATGATTGCT+TGG 0.533846 4:+48938944 MsaT020293.1:CDS
GACCCTCCTTCTCCTCCCAT+TGG 0.534808 4:-48940942 None:intergenic
TCTGAGAGAGAAATCCATGT+TGG 0.535848 4:-48941304 None:intergenic
GAAACTTCATATGGTTGTGT+AGG 0.538056 4:-48936575 None:intergenic
TAAACGCCAGAGTTCGCTTG+TGG 0.538237 4:+48940712 MsaT020293.1:CDS
AATGGTTGAAGAAATGTACT+TGG 0.541831 4:+48940740 MsaT020293.1:CDS
TGAAAGGAAACTACCTTGAT+GGG 0.542083 4:-48941030 None:intergenic
GTCGGCCTCTGGTGAGATTG+AGG 0.544425 4:+48936841 MsaT020293.1:CDS
TTATCCCAAGGACTCCGATA+AGG 0.544643 4:+48939897 MsaT020293.1:CDS
CATCAGATGATCCTGCAAGA+TGG 0.545078 4:-48940986 None:intergenic
TGGAAGGTGGAAACTTCATA+TGG 0.546476 4:-48936584 None:intergenic
CGTGGAGGCGAGGAAGGTGG+TGG 0.547728 4:+48936878 MsaT020293.1:CDS
ATAACCTTATCGGAGTCCTT+GGG 0.549058 4:-48939901 None:intergenic
ATGAGAAGGAGTAGTGTTGA+TGG 0.552006 4:-48941384 None:intergenic
TTCAACTTCAAACTCTGTGG+TGG 0.552624 4:-48941084 None:intergenic
CAATCTCACCAGAGGCCGAC+CGG 0.552708 4:-48936838 None:intergenic
TCCTCGCCTCCACGGCTACC+CGG 0.553350 4:-48936869 None:intergenic
ACATCACCAATGGGAGGAGA+AGG 0.558319 4:+48940936 MsaT020293.1:CDS
TTAGAGAAACACCATTTCCA+TGG 0.559032 4:-48941238 None:intergenic
TCAATGTCAACATCACCAAT+GGG 0.559905 4:+48940927 MsaT020293.1:CDS
GTAAACCTTCTACCGACGGT+TGG 0.560766 4:-48936322 None:intergenic
TATGAAAACCAATTTGCTGC+GGG 0.560781 4:-48936760 None:intergenic
TAATTTGTTCCTGAATGAGA+AGG 0.560909 4:-48941398 None:intergenic
CTCGTCCCCACTATAACATG+TGG 0.566142 4:+48936636 MsaT020293.1:CDS
AGCCGTGGAGGCGAGGAAGG+TGG 0.567527 4:+48936875 MsaT020293.1:CDS
GGAAGAAACCAAGGAGCAAG+AGG 0.569522 4:+48940761 MsaT020293.1:CDS
TCACCAATGGGAGGAGAAGG+AGG 0.571505 4:+48940939 MsaT020293.1:CDS
GAACAATGTAGGTAGAGCTA+AGG 0.571744 4:+48938754 MsaT020293.1:intron
ACGAGGAGTCACGGTAGCCG+AGG 0.573764 4:-48936618 None:intergenic
TGATCCCCACATGTTATAGT+GGG 0.573944 4:-48936642 None:intergenic
TGGTTATGGATCATTCTCAA+TGG 0.575128 4:+48941169 MsaT020293.1:CDS
AGTTCGCTTGTGGAAACCAA+TGG 0.575659 4:+48940722 MsaT020293.1:CDS
GTAATAACAACAAAGAGAAG+AGG 0.583452 4:+48937026 MsaT020293.1:CDS
AATATTGAATCTAGTTCAAG+TGG 0.585962 4:+48937055 MsaT020293.1:CDS
TGATTCATAGCAATGCTTGG+AGG 0.588232 4:+48939045 MsaT020293.1:CDS
TGGTGTTGATTGAGTGATGA+AGG 0.588479 4:-48936494 None:intergenic
GTGGATAAGGGAATCATCAA+AGG 0.589254 4:+48936980 MsaT020293.1:CDS
CACCACCTTCCTCGCCTCCA+CGG 0.590288 4:-48936877 None:intergenic
CACATGTTATAGTGGGGACG+AGG 0.591269 4:-48936635 None:intergenic
AGAGGAGAATAATGACTCCA+AGG 0.594942 4:+48940779 MsaT020293.1:CDS
AATCATCAAAGGAGAATCTG+TGG 0.597233 4:+48936991 MsaT020293.1:CDS
TCACACTTTGCATTTGATGG+TGG 0.598415 4:-48938788 None:intergenic
CGAGGAGGAAAACGGATGGA+AGG 0.601970 4:-48936600 None:intergenic
CTTGAATCCATGAAGCGCGT+GGG 0.602989 4:-48936459 None:intergenic
ATATGGTTGTGTAGGAGTGT+CGG 0.603447 4:-48936567 None:intergenic
GGAGGAAAACGGATGGAAGG+TGG 0.603654 4:-48936597 None:intergenic
GAGGTATCTCCGGGTAGCCG+TGG 0.603840 4:+48936860 MsaT020293.1:CDS
ACACCATTTCCATGGAACCT+AGG 0.604931 4:-48941230 None:intergenic
CAGGATCATCTGATGTGCAA+AGG 0.605084 4:+48940994 MsaT020293.1:CDS
GATCCATGAAAGGTGGAAGG+TGG 0.606700 4:-48936536 None:intergenic
GGTGGAGTTTCGACCATGAA+CGG 0.607341 4:+48936896 MsaT020293.1:CDS
GTCTGTAAACCTTCTACCGA+CGG 0.607577 4:-48936326 None:intergenic
ATCAAAGGAGAATCTGTGGA+AGG 0.608300 4:+48936995 MsaT020293.1:CDS
CCATGTTGGGTTGAAGAGAG+AGG 0.608631 4:-48941290 None:intergenic
TCGTCCCCACTATAACATGT+GGG 0.609880 4:+48936637 MsaT020293.1:CDS
ACTTGAATCCATGAAGCGCG+TGG 0.612796 4:-48936460 None:intergenic
CTTTCAGGCAAGCCTCTCTG+AGG 0.615305 4:-48939068 None:intergenic
GGTGGAAGAGAAAACAATGG+AGG 0.618595 4:+48937076 MsaT020293.1:CDS
CTATGAAAACCAATTTGCTG+CGG 0.619359 4:-48936761 None:intergenic
TCTTGATGAAGTTGTGAATG+TGG 0.620303 4:+48936961 MsaT020293.1:CDS
ATTGAATCTAGTTCAAGTGG+TGG 0.621430 4:+48937058 MsaT020293.1:CDS
TTGAATCCATGAAGCGCGTG+GGG 0.624444 4:-48936458 None:intergenic
AATGAAAATCATCATGGTGG+TGG 0.625687 4:+48941149 MsaT020293.1:CDS
AATGTACTTGGAAGAAACCA+AGG 0.627170 4:+48940752 MsaT020293.1:CDS
AGTGGAAATGAAAATCATCA+TGG 0.627811 4:+48941143 MsaT020293.1:CDS
AGGAGTCACGGTAGCCGAGG+AGG 0.628325 4:-48936615 None:intergenic
GCTTGTTAGCATGCTAGATG+AGG 0.632117 4:+48937159 MsaT020293.1:CDS
GTATCTCCGGGTAGCCGTGG+AGG 0.632306 4:+48936863 MsaT020293.1:CDS
GTGGAAGAGAAAACAATGGA+GGG 0.633833 4:+48937077 MsaT020293.1:CDS
ATTGAGTGATGAAGGCGCGT+GGG 0.637713 4:-48936486 None:intergenic
ATAGTGGGGACGAGGAGTCA+CGG 0.640127 4:-48936627 None:intergenic
AGTGTTGGATCCATGAAAGG+TGG 0.640356 4:-48936543 None:intergenic
CATAGACCAGTTTATCAGCG+TGG 0.640750 4:+48936779 MsaT020293.1:CDS
TGTGACCTGCAGTTATCCCA+AGG 0.642673 4:+48939885 MsaT020293.1:intron
CACCAATGGGAGGAGAAGGA+GGG 0.642966 4:+48940940 MsaT020293.1:CDS
CCTTTCAACTTCAAACTCTG+TGG 0.643049 4:-48941087 None:intergenic
GAATAGTAACTCCAAGCAAG+TGG 0.643953 4:-48936514 None:intergenic
AAAGGAGAATCTGTGGAAGG+AGG 0.648166 4:+48936998 MsaT020293.1:CDS
TAGCCGAGGAGGAAAACGGA+TGG 0.650470 4:-48936604 None:intergenic
GGTAGTGTTGGATCCATGAA+AGG 0.655484 4:-48936546 None:intergenic
GATTGAGTGATGAAGGCGCG+TGG 0.659202 4:-48936487 None:intergenic
CGTCCCCACTATAACATGTG+GGG 0.666378 4:+48936638 MsaT020293.1:CDS
CAGTTCTGAAATCCAACCGT+CGG 0.674796 4:+48936310 MsaT020293.1:CDS
ACCACCAACTCCGACAACAG+CGG 0.680001 4:+48939003 MsaT020293.1:CDS
TTGGATCCATGAAAGGTGGA+AGG 0.684588 4:-48936539 None:intergenic
AGATACCTCAATCTCACCAG+AGG 0.686383 4:-48936846 None:intergenic
GCAACAAACGCCTTACCGGT+CGG 0.687220 4:+48936823 MsaT020293.1:CDS
TTGAGTGATGAAGGCGCGTG+GGG 0.689955 4:-48936485 None:intergenic
ATGTCAACATCACCAATGGG+AGG 0.690189 4:+48940930 MsaT020293.1:CDS
AGTGGTGGAAGAGAAAACAA+TGG 0.709372 4:+48937073 MsaT020293.1:CDS
GATCCCCACATGTTATAGTG+GGG 0.711349 4:-48936641 None:intergenic
AAACTACGTACCTTACTCTG+AGG 0.712519 4:+48936367 MsaT020293.1:CDS
AATGCTTGGAGGCCTCAGAG+AGG 0.737120 4:+48939056 MsaT020293.1:CDS
GGAAATGAAAATCATCATGG+TGG 0.766816 4:+48941146 MsaT020293.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 48936275 48941487 48936275 ID=MsaG020293
Chr4 mRNA 48936275 48941487 48936275 ID=MsaT020293.1;Parent=MsaG020293
Chr4 exon 48936275 48937180 48936275 ID=MsaT020293.1.exon1;Parent=MsaT020293.1
Chr4 CDS 48936275 48937180 48936275 ID=cds.MsaT020293.1;Parent=MsaT020293.1
Chr4 exon 48938765 48939138 48938765 ID=MsaT020293.1.exon2;Parent=MsaT020293.1
Chr4 CDS 48938765 48939138 48938765 ID=cds.MsaT020293.1;Parent=MsaT020293.1
Chr4 exon 48939897 48939957 48939897 ID=MsaT020293.1.exon3;Parent=MsaT020293.1
Chr4 CDS 48939897 48939957 48939897 ID=cds.MsaT020293.1;Parent=MsaT020293.1
Chr4 exon 48940696 48941487 48940696 ID=MsaT020293.1.exon4;Parent=MsaT020293.1
Chr4 CDS 48940696 48941487 48940696 ID=cds.MsaT020293.1;Parent=MsaT020293.1
Gene Sequence

>MsaT020293.1

ATGGCAACGTACTTTCATGGCAACAGCAACAGCAACAGTTCTGAAATCCAACCGTCGGTAGAAGGTTTACAGACACTTTACCTTATGAATCCAAACTACGTACCTTACTCTGAGGAACCACAAAATCCAACACAAAACATGCTTTTCGCTAATCCCAACGCTACCAATAGCAATAATGGTTCTCCCCACGCGCTTCATGGATTCAAGTTTCCCCACGCGCCTTCATCACTCAATCAACACCACTTGCTTGGAGTTACTATTCCACCTTCCACCTTTCATGGATCCAACACTACCGACACTCCTACACAACCATATGAAGTTTCCACCTTCCATCCGTTTTCCTCCTCGGCTACCGTGACTCCTCGTCCCCACTATAACATGTGGGGATCAGGTAGCTTTCTACCTGATAACGCTGTCACAGCAACGACAACTGATAGATCAGAATGTTCAGATGTCGTTTCTGTGACAGCATCTCCAGAGTTTCGCCCGCAGCAAATTGGTTTTCATAGACCAGTTTATCAGCGTGGTTTGTCTCTCAGTCTTTCTTCGCAACAAACGCCTTACCGGTCGGCCTCTGGTGAGATTGAGGTATCTCCGGGTAGCCGTGGAGGCGAGGAAGGTGGTGGAGTTTCGACCATGAACGGTGTTATTTTTGGCTCGAAGTATATGAAAGTAGCACAAGAGCTTCTTGATGAAGTTGTGAATGTGGATAAGGGAATCATCAAAGGAGAATCTGTGGAAGGAGGTAATAGTAATAACAACAAAGAGAAGAGGAAAGCAAATATTGAATCTAGTTCAAGTGGTGGAAGAGAAAACAATGGAGGGAAACAAGTAGCTGAATTAAGCACTGCACAAAGACAAGAACTTCAGATGAAGAAGTCAAAGCTTGTTAGCATGCTAGATGAGGTAGAGCTAAGGTACAGACAATACCACCATCAAATGCAAAGTGTGATATCATCATTTGAGCAAGCAACAGGATATGGAGCATCAAAATCTTACACATCCCTAGCCTTGAAGACAATCTCAAAGCAGTTTCGAAGTCTAAAAGATTCAATCTCTTCACAAATTAAGACAGCTAGCAAGACTTTGGGTGAAGATGATTGCTTGGGTGTTAAGTTAGAAGGTTCTAGGCTTCGGTATGTCGACCACCAACTCCGACAACAGCGGGCACTGCAGCAACTTGGAATGATTCATAGCAATGCTTGGAGGCCTCAGAGAGGCTTGCCTGAAAGAGCTGTTTCTGTTCTTCGTGCTTGGCTTTTTGAACATTTCTTGCACCCTTATCCCAAGGACTCCGATAAGGTTATGCTTGCAAGACAAACTGGACTTACTAGGAGTCAGGTATCTAACTGGTTTATAAACGCCAGAGTTCGCTTGTGGAAACCAATGGTTGAAGAAATGTACTTGGAAGAAACCAAGGAGCAAGAGGAGAATAATGACTCCAAGGATAACACAAATAGATCAAAAGAATTAGGTGCCACAAAACTTGATCAAATTAATAATCTTCATTCAAAAGCAGAAAACTTCAACAACCAAAACCTTTCTCCAACAGTGATTTCAAGTTCCAATAATTCAATGTCAACATCACCAATGGGAGGAGAAGGAGGGTCCTTTCAATCCAATTCTGGTTTCCATCTTGCAGGATCATCTGATGTGCAAAGGAGTCCAAAAAACTCCCATCAAGGTAGTTTCCTTTCAATGGACATGGAATTGAAGTCTAATAGAGAGTCCACCACAGAGTTTGAAGTTGAAAGGCATAATCATGACAAAAATGAATACCCTTTATTGAGTGGAAATGAAAATCATCATGGTGGTGGTTATGGATCATTCTCAATGGAAGATGTTAGAAGGTTTAATGTTAGTGAGCAATTAGCTCCTAGGTTCCATGGAAATGGTGTTTCTCTAACTCTTGGACTACCTCATAATGAAAATATTCCTCTCTCTTCAACCCAACATGGATTTCTCTCTCAGAATTTACATTTAGGAGGGACGATTGATGAAATGGGAAGCAATGAGAATGAGTTTTGTACCATCAACACTACTCCTTCTCATTCAGGAACAAATTATGAGAGTATTGATATTGAAAACAGAAAGAGGTTTGCTGCTCAATTATTGCGAAATTTTGTGACCTAG
Protein sequence

>MsaT020293.1

MATYFHGNSNSNSSEIQPSVEGLQTLYLMNPNYVPYSEEPQNPTQNMLFANPNATNSNNGSPHALHGFKFPHAPSSLNQHHLLGVTIPPSTFHGSNTTDTPTQPYEVSTFHPFSSSATVTPRPHYNMWGSGSFLPDNAVTATTTDRSECSDVVSVTASPEFRPQQIGFHRPVYQRGLSLSLSSQQTPYRSASGEIEVSPGSRGGEEGGGVSTMNGVIFGSKYMKVAQELLDEVVNVDKGIIKGESVEGGNSNNNKEKRKANIESSSSGGRENNGGKQVAELSTAQRQELQMKKSKLVSMLDEVELRYRQYHHQMQSVISSFEQATGYGASKSYTSLALKTISKQFRSLKDSISSQIKTASKTLGEDDCLGVKLEGSRLRYVDHQLRQQRALQQLGMIHSNAWRPQRGLPERAVSVLRAWLFEHFLHPYPKDSDKVMLARQTGLTRSQVSNWFINARVRLWKPMVEEMYLEETKEQEENNDSKDNTNRSKELGATKLDQINNLHSKAENFNNQNLSPTVISSSNNSMSTSPMGGEGGSFQSNSGFHLAGSSDVQRSPKNSHQGSFLSMDMELKSNRESTTEFEVERHNHDKNEYPLLSGNENHHGGGYGSFSMEDVRRFNVSEQLAPRFHGNGVSLTLGLPHNENIPLSSTQHGFLSQNLHLGGTIDEMGSNENEFCTINTTPSHSGTNYESIDIENRKRFAAQLLRNFVT*