AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020407


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020407 MtrunA17_Chr4g0026521 89.837 246 22 1 9 251 50 295 1.42e-150 421
MsaG020407 MtrunA17_Chr2g0321291 76.033 242 42 6 22 249 68 307 2.93e-126 360
MsaG020407 MtrunA17_Chr2g0328921 68.224 214 60 2 16 222 74 286 4.09e-108 314
MsaG020407 MtrunA17_Chr8g0352011 66.063 221 61 4 31 238 110 329 1.77e-104 306
MsaG020407 MtrunA17_Chr1g0173841 61.355 251 67 6 23 246 137 384 2.01e-104 308
MsaG020407 MtrunA17_Chr7g0258011 63.043 230 65 3 34 246 119 345 4.23e-103 303
MsaG020407 MtrunA17_Chr5g0422571 62.996 227 69 5 26 247 55 271 1.22e-102 300
MsaG020407 MtrunA17_Chr1g0198651 72.989 174 41 3 62 229 89 262 4.78e-95 280
MsaG020407 MtrunA17_Chr4g0053341 65.464 194 60 1 29 222 36 222 4.00e-93 273
MsaG020407 MtrunA17_Chr1g0200421 38.415 164 89 6 81 236 245 404 1.36e-25 104
MsaG020407 MtrunA17_Chr2g0320341 34.911 169 100 7 74 235 231 396 1.55e-22 95.1
MsaG020407 MtrunA17_Chr4g0029521 36.691 139 79 4 81 218 61 191 7.37e-20 88.2
MsaG020407 MtrunA17_Chr2g0326381 31.818 176 109 4 57 228 31 199 8.32e-20 87.8
MsaG020407 MtrunA17_Chr1g0181381 34.932 146 86 4 74 218 54 191 1.09e-19 87.8
MsaG020407 MtrunA17_Chr8g0382121 31.217 189 122 6 39 222 78 263 1.70e-19 85.9
MsaG020407 MtrunA17_Chr1g0151351 35.461 141 82 4 79 218 62 194 5.07e-19 85.5
MsaG020407 MtrunA17_Chr3g0123941 36.810 163 91 7 89 247 139 293 7.10e-19 84.3
MsaG020407 MtrunA17_Chr7g0263941 36.054 147 82 6 74 218 50 186 7.18e-19 85.1
MsaG020407 MtrunA17_Chr1g0185121 34.694 147 85 5 74 218 56 193 8.50e-19 85.1
MsaG020407 MtrunA17_Chr7g0268961 35.252 139 80 4 81 218 45 174 9.43e-19 85.1
MsaG020407 MtrunA17_Chr8g0373541 32.558 172 98 7 56 218 23 185 1.60e-18 84.3
MsaG020407 MtrunA17_Chr1g0191991 34.694 147 84 5 74 218 79 215 3.60e-18 83.6
MsaG020407 MtrunA17_Chr8g0342421 30.435 184 108 7 45 218 41 214 3.71e-18 83.2
MsaG020407 MtrunA17_Chr2g0330171 31.707 164 100 5 57 218 45 198 1.09e-17 82.0
MsaG020407 MtrunA17_Chr1g0210821 34.014 147 85 5 74 218 60 196 1.14e-17 82.0
MsaG020407 MtrunA17_Chr1g0199101 34.014 147 85 6 74 218 63 199 1.89e-17 81.3
MsaG020407 MtrunA17_Chr2g0324991 34.507 142 83 5 74 213 8 141 2.25e-17 76.6
MsaG020407 MtrunA17_Chr3g0141431 33.129 163 91 5 64 216 20 174 6.09e-17 79.3
MsaG020407 MtrunA17_Chr1g0162971 34.014 147 85 5 74 218 57 193 6.36e-17 79.7
MsaG020407 MtrunA17_Chr1g0163001 33.333 147 86 5 74 218 59 195 1.09e-16 79.0
MsaG020407 MtrunA17_Chr4g0040571 31.507 146 90 4 74 218 55 191 1.91e-15 75.5
MsaG020407 MtrunA17_Chr8g0375521 30.935 139 85 5 79 214 48 178 4.74e-12 65.1
MsaG020407 MtrunA17_Chr6g0479921 27.607 163 106 5 81 241 52 204 9.16e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020407 AT1G08290.1 83.815 173 27 1 70 241 163 335 9.29e-109 317
MsaG020407 AT3G57670.1 83.553 152 25 0 71 222 201 352 8.06e-100 296
MsaG020407 AT1G51220.1 82.781 151 26 0 72 222 163 313 4.38e-99 293
MsaG020407 AT3G20880.1 73.184 179 44 1 69 247 238 412 5.90e-99 295
MsaG020407 AT1G13290.1 70.157 191 48 4 34 222 55 238 1.90e-96 285
MsaG020407 AT1G34790.1 61.009 218 74 3 35 244 85 299 5.03e-91 271
MsaG020407 AT1G34370.3 39.735 151 79 5 81 222 28 175 1.26e-26 105
MsaG020407 AT1G34370.2 39.735 151 79 5 81 222 238 385 1.12e-25 105
MsaG020407 AT1G34370.1 39.735 151 79 5 81 222 238 385 1.12e-25 105
MsaG020407 AT5G22890.1 35.976 164 96 5 62 218 194 355 1.33e-23 98.2
MsaG020407 AT5G03150.1 32.317 164 100 5 57 218 52 206 6.93e-20 88.6
MsaG020407 AT5G66730.1 35.461 141 81 4 79 218 53 184 3.81e-19 86.7
MsaG020407 AT1G55110.3 29.255 188 119 6 33 218 41 216 8.48e-19 85.5
MsaG020407 AT1G55110.1 29.255 188 119 6 33 218 41 216 8.48e-19 85.5
MsaG020407 AT1G55110.2 29.255 188 119 6 33 218 41 216 8.48e-19 85.5
MsaG020407 AT5G60470.1 36.691 139 79 4 81 218 58 188 1.96e-18 84.3
MsaG020407 AT5G60470.3 35.971 139 80 4 81 218 63 193 2.46e-18 84.0
MsaG020407 AT5G60470.2 35.971 139 80 4 81 218 67 197 2.71e-18 84.0
MsaG020407 AT5G44160.1 29.282 181 115 5 42 218 18 189 4.06e-18 83.6
MsaG020407 AT3G45260.2 35.971 139 80 4 81 218 62 192 5.72e-18 82.8
MsaG020407 AT3G45260.1 35.971 139 80 4 81 218 62 192 5.72e-18 82.8
MsaG020407 AT2G02070.2 31.481 162 101 4 58 218 52 204 7.48e-18 82.8
MsaG020407 AT2G02070.1 31.481 162 101 4 58 218 52 204 7.48e-18 82.8
MsaG020407 AT1G14580.1 31.902 163 99 5 58 218 53 205 8.89e-18 82.4
MsaG020407 AT1G14580.2 31.902 163 99 5 58 218 53 205 8.89e-18 82.4
MsaG020407 AT1G14580.3 31.902 163 99 5 58 218 67 219 8.92e-18 82.4
MsaG020407 AT2G02080.1 30.864 162 102 4 58 218 54 206 1.25e-17 82.0
MsaG020407 AT2G02080.4 30.864 162 102 4 58 218 54 206 1.25e-17 82.0
MsaG020407 AT2G02080.5 30.864 162 102 4 58 218 54 206 1.25e-17 82.0
MsaG020407 AT2G02080.3 30.864 162 102 4 58 218 54 206 1.25e-17 82.0
MsaG020407 AT3G13810.5 30.864 162 103 4 58 218 65 218 2.20e-17 81.6
MsaG020407 AT3G13810.4 30.864 162 103 4 58 218 70 223 2.21e-17 81.6
MsaG020407 AT3G13810.1 30.864 162 103 4 58 218 70 223 2.21e-17 81.6
MsaG020407 AT3G13810.6 30.864 162 103 4 58 218 51 204 2.43e-17 81.3
MsaG020407 AT3G13810.3 33.562 146 88 4 74 218 73 210 2.70e-17 81.3
MsaG020407 AT3G13810.2 33.562 146 88 4 74 218 87 224 3.09e-17 80.9
MsaG020407 AT1G03840.1 31.288 163 102 4 57 218 40 193 3.36e-17 80.9
MsaG020407 AT3G50700.1 33.562 146 87 4 74 218 50 186 3.55e-17 80.9
MsaG020407 AT2G02080.6 35.821 134 76 4 86 218 5 129 3.94e-17 80.5
MsaG020407 AT2G02080.2 35.821 134 76 4 86 218 5 129 3.94e-17 80.5
MsaG020407 AT4G02670.1 34.247 146 87 4 74 218 69 206 4.85e-17 80.1
MsaG020407 AT4G02670.2 34.247 146 87 4 74 218 71 208 5.39e-17 80.1
MsaG020407 AT1G03840.2 32.877 146 88 4 74 218 55 191 7.07e-17 80.1
MsaG020407 AT5G44160.2 32.192 146 89 4 74 218 14 150 9.15e-17 79.3
MsaG020407 AT1G68130.1 29.375 160 97 6 74 225 57 208 7.35e-13 68.2
MsaG020407 AT1G25250.1 27.972 143 95 4 74 214 26 162 4.07e-12 65.5
MsaG020407 AT1G25250.3 27.972 143 95 4 74 214 49 185 7.11e-12 65.1
MsaG020407 AT2G01940.1 30.216 139 86 5 79 214 65 195 7.73e-11 62.0

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATCATATTAGGTCTTT+TGG 0.190747 4:+51299487 MsaT020407.1:CDS
CCATGCTACTGTTGTGTTCA+AGG 0.271424 4:+51299247 MsaT020407.1:CDS
GAGGAGAAGGGATGTGTTAC+TGG 0.312071 4:+51299556 MsaT020407.1:CDS
AAACTTCTTCTCATGAAGAT+AGG 0.335592 4:-51295937 None:intergenic
GATCTCTCAAAGATCATATT+AGG 0.336377 4:+51299479 MsaT020407.1:CDS
GAGACTGTGGCTTTGCATAT+TGG 0.342224 4:+51295894 MsaT020407.1:CDS
CATGTTGTTATACCTATTGA+AGG 0.383480 4:-51296081 None:intergenic
AACTCAAAACCCTCAATTTG+TGG 0.388963 4:-51295858 None:intergenic
TCTTTCAAAGGCTTAGCTCT+TGG 0.410923 4:-51299292 None:intergenic
CATTTGCTGTCAAAGGTGAT+TGG 0.415877 4:+51299389 MsaT020407.1:CDS
TGTGTGCCTCTTAGTGAGTC+TGG 0.423624 4:-51299202 None:intergenic
AAAAGAATTGTGGCAAGTTA+TGG 0.431308 4:+51299422 MsaT020407.1:CDS
ACTCCTGCTCAGATCCTTGT+TGG 0.433316 4:+51296023 MsaT020407.1:CDS
TATGCAACAAAACCTTCAAT+AGG 0.434246 4:+51296069 MsaT020407.1:CDS
GCGTGAAGTGCCACAAATTG+AGG 0.454416 4:+51295848 MsaT020407.1:CDS
GAGTTGGTATCCAAAACCTT+TGG 0.485962 4:-51296004 None:intergenic
CCATTTATGTGTAGAAAATG+TGG 0.488125 4:+51299361 MsaT020407.1:CDS
GGTAGCCTTAGCATTGCAGC+TGG 0.491825 4:-51299226 None:intergenic
CGTGAAGTGCCACAAATTGA+GGG 0.493265 4:+51295849 MsaT020407.1:CDS
GAGGGAATCAACTGCATGGA+AGG 0.515358 4:+51293652 MsaT020407.1:CDS
TCAAGCTTTCGAAGAGGAGA+AGG 0.518310 4:+51299543 MsaT020407.1:CDS
AAGTTATGGTATTGCACTTG+TGG 0.518903 4:+51299436 MsaT020407.1:CDS
TTTCTTCTTCTTCTTTAACA+TGG 0.521689 4:-51295962 None:intergenic
TTTCCATTAGATGCATATGT+GGG 0.526101 4:+51299153 MsaT020407.1:intron
TTGCTGTCAAAGGTGATTGG+AGG 0.526560 4:+51299392 MsaT020407.1:CDS
CACAACCAGCTGCAATGCTA+AGG 0.533499 4:+51299221 MsaT020407.1:CDS
AGAGTTCTAAAGTCTTTCAA+AGG 0.540762 4:-51299304 None:intergenic
TGGTATCCAAAACCTTTGGA+AGG 0.554935 4:-51296000 None:intergenic
GGTCCAACAAGGATCTGAGC+AGG 0.564499 4:-51296026 None:intergenic
TCCATTAGATGCATATGTGG+GGG 0.571799 4:+51299155 MsaT020407.1:intron
ACAAGGATCTGAGCAGGAGT+TGG 0.574558 4:-51296020 None:intergenic
AAAGGACCAGACTCACTAAG+AGG 0.575770 4:+51299196 MsaT020407.1:CDS
TTCCATTAGATGCATATGTG+GGG 0.605042 4:+51299154 MsaT020407.1:intron
CATTTATGTGTAGAAAATGT+GGG 0.610048 4:+51299362 MsaT020407.1:CDS
ACTCACTACAAAAGAAAACA+TGG 0.615968 4:+51299331 MsaT020407.1:CDS
CAAGCTTTCGAAGAGGAGAA+GGG 0.617403 4:+51299544 MsaT020407.1:CDS
AAAGCTTGATCATCAATCGA+AGG 0.619722 4:-51299529 None:intergenic
CAAATTGCATAGGTCCAACA+AGG 0.619822 4:-51296037 None:intergenic
CAATAGGTATAACAACATGC+AGG 0.621278 4:+51296085 MsaT020407.1:CDS
ATGGAGCAAGCAAATTGCAT+AGG 0.622517 4:-51296047 None:intergenic
AGGACTCATGAAAAGAATTG+TGG 0.623632 4:+51299412 MsaT020407.1:CDS
GGGAAAACATTTGCTGTCAA+AGG 0.623807 4:+51299382 MsaT020407.1:CDS
CATGGATCTGAATTTAGGAA+AGG 0.631532 4:+51299178 MsaT020407.1:CDS
TGATGATCAAGCTTTCGAAG+AGG 0.631618 4:+51299537 MsaT020407.1:CDS
AGGGAATCAACTGCATGGAA+GGG 0.650257 4:+51293653 MsaT020407.1:CDS
TATGGAGGGAATCAACTGCA+TGG 0.650662 4:+51293648 None:intergenic
CCTTGAACACAACAGTAGCA+TGG 0.671135 4:-51299247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 51293649 51299609 51293649 ID=MsaG020407
Chr4 mRNA 51293649 51299609 51293649 ID=MsaT020407.1;Parent=MsaG020407
Chr4 exon 51293649 51293674 51293649 ID=MsaT020407.1.exon1;Parent=MsaT020407.1
Chr4 CDS 51293649 51293674 51293649 ID=cds.MsaT020407.1;Parent=MsaT020407.1
Chr4 exon 51295824 51296106 51295824 ID=MsaT020407.1.exon2;Parent=MsaT020407.1
Chr4 CDS 51295824 51296106 51295824 ID=cds.MsaT020407.1;Parent=MsaT020407.1
Chr4 exon 51299163 51299609 51299163 ID=MsaT020407.1.exon3;Parent=MsaT020407.1
Chr4 CDS 51299163 51299609 51299163 ID=cds.MsaT020407.1;Parent=MsaT020407.1
Gene Sequence

>MsaT020407.1

ATGGAGGGAATCAACTGCATGGAAGGTGCAAAGTTTTCAAAGGATGAAGAGCGTGAAGTGCCACAAATTGAGGGTTTTGAGTTCAAAGAAGAAAAAGAGACTGTGGCTTTGCATATTGGATTACCTCATGACACTAAAACCTATCTTCATGAGAAGAAGTTTTTCCATGTTAAAGAAGAAGAAGAAAAGCCTTCTAAAAAAACCTTCCAAAGGTTTTGGATACCAACTCCTGCTCAGATCCTTGTTGGACCTATGCAATTTGCTTGCTCCATATGCAACAAAACCTTCAATAGGTATAACAACATGCAGATGCATATGTGGGGGCATGGATCTGAATTTAGGAAAGGACCAGACTCACTAAGAGGCACACAACCAGCTGCAATGCTAAGGCTACCATGCTACTGTTGTGTTCAAGGTTGCAAGAACAACATCAATCATCCAAGAGCTAAGCCTTTGAAAGACTTTAGAACTCTCCAAACTCACTACAAAAGAAAACATGGAACAAAACCATTTATGTGTAGAAAATGTGGGAAAACATTTGCTGTCAAAGGTGATTGGAGGACTCATGAAAAGAATTGTGGCAAGTTATGGTATTGCACTTGTGGTTCAGATTTTAAACACAAAAGATCTCTCAAAGATCATATTAGGTCTTTTGGAAAAGGTCATCATCGTCTTCCTTCGATTGATGATCAAGCTTTCGAAGAGGAGAAGGGATGTGTTACTGGCTCTGAAGAAGATGAATTTGCTCGTACATGA

Protein sequence

>MsaT020407.1

MEGINCMEGAKFSKDEEREVPQIEGFEFKEEKETVALHIGLPHDTKTYLHEKKFFHVKEEEEKPSKKTFQRFWIPTPAQILVGPMQFACSICNKTFNRYNNMQMHMWGHGSEFRKGPDSLRGTQPAAMLRLPCYCCVQGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFMCRKCGKTFAVKGDWRTHEKNCGKLWYCTCGSDFKHKRSLKDHIRSFGKGHHRLPSIDDQAFEEEKGCVTGSEEDEFART*