AlfalfaGEDB | MsaG020459

M. sativa ssp. caerulea / MsaG020459

KEGG: K08850
GO: GO:0000226 GO:0000228 GO:0000278 GO:0000775 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000922 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005694 GO:0005730 GO:0005737 GO:0005819 GO:0005856 GO:0005874 GO:0005875 GO:0005876 GO:0006325 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0007052 GO:0008150 GO:0008152 GO:0009504 GO:0009524 GO:0009987 GO:0010564 GO:0012505 GO:0015630 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022402 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032133 GO:0032465 GO:0032991 GO:0035173 GO:0035174 GO:0035175 GO:0035404 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043987 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0050789 GO:0050794 GO:0051233 GO:0051276 GO:0051302 GO:0051726 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0098687 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140096 GO:1901564 GO:1902850 GO:1903047
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020459	TQD68854.1	84.058	69	11	0	26	94	38	106	1.65e-37	133

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020459	sp\|Q683C9\|AUR2_ARATH	84.058	69	11	0	26	94	122	190	6.36e-37	129

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020459	tr\|A0A540K3Q6\|A0A540K3Q6_MALBA	84.058	69	11	0	26	94	38	106	7.86e-38	133

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG020458	MsaG020459	0.853233	5.260226e-61	3.190940e-58

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020459	MtrunA17_Chr3g0140631	84.058	69	11	0	26	94	140	208	8.34e-38	129
MsaG020459	MtrunA17_Chr8g0351491	57.895	76	32	0	22	97	115	190	5.67e-26	97.8
MsaG020459	MtrunA17_Chr8g0353621	56.579	76	33	0	22	97	109	184	4.39e-25	95.5
MsaG020459	MtrunA17_Chr3g0125381	55.696	79	32	1	22	97	51	129	1.52e-24	92.8
MsaG020459	MtrunA17_Chr3g0114511	47.887	71	32	1	22	87	128	198	1.09e-13	65.9
MsaG020459	MtrunA17_Chr3g0091311	47.143	70	33	1	22	87	110	179	1.50e-13	64.7
MsaG020459	MtrunA17_Chr1g0205421	41.558	77	40	1	16	87	104	180	2.06e-13	65.1
MsaG020459	MtrunA17_Chr8g0346071	40.260	77	43	1	14	87	108	184	3.04e-13	64.7
MsaG020459	MtrunA17_Chr3g0091421	47.143	70	33	1	22	87	110	179	3.07e-13	63.5
MsaG020459	MtrunA17_Chr3g0099951	50.877	57	28	0	12	68	104	160	4.44e-13	63.9
MsaG020459	MtrunA17_Chr8g0346081	41.096	73	40	1	18	87	112	184	5.19e-13	63.9
MsaG020459	MtrunA17_Chr4g0059751	35.443	79	47	2	11	87	186	262	3.92e-12	61.2
MsaG020459	MtrunA17_Chr3g0091291	44.286	70	35	1	22	87	58	127	6.03e-12	59.7
MsaG020459	MtrunA17_Chr5g0428031	38.356	73	42	1	18	87	104	176	1.17e-11	60.1
MsaG020459	MtrunA17_Chr6g0483151	47.273	55	29	0	12	66	102	156	1.55e-11	59.7
MsaG020459	MtrunA17_Chr4g0063881	45.455	66	33	1	25	87	113	178	1.79e-11	59.3
MsaG020459	MtrunA17_Chr3g0107051	44.928	69	31	2	25	87	122	189	2.42e-11	59.3
MsaG020459	MtrunA17_Chr7g0244521	41.176	68	35	1	25	87	113	180	5.56e-11	58.2
MsaG020459	MtrunA17_Chr2g0304111	40.000	75	40	1	18	87	118	192	7.72e-11	57.8
MsaG020459	MtrunA17_Chr4g0013131	42.647	68	34	1	25	87	113	180	8.28e-11	57.8
MsaG020459	MtrunA17_Chr3g0091281	45.714	70	34	1	22	87	110	179	9.03e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020459	AT2G25880.1	84.058	69	11	0	26	94	128	196	6.73e-38	129
MsaG020459	AT4G32830.1	84.058	69	11	0	26	94	134	202	9.55e-38	129
MsaG020459	AT2G25880.4	84.058	69	11	0	26	94	141	209	1.07e-37	129
MsaG020459	AT2G25880.3	84.058	69	11	0	26	94	189	257	4.52e-37	128
MsaG020459	AT2G45490.2	55.422	83	37	0	15	97	114	196	6.69e-27	100
MsaG020459	AT2G45490.1	55.422	83	37	0	15	97	114	196	1.33e-26	100
MsaG020459	AT2G25880.2	70.000	40	12	0	55	94	125	164	3.60e-14	66.6
MsaG020459	AT4G24400.2	39.286	84	46	2	7	87	95	176	4.26e-13	64.3
MsaG020459	AT1G73690.1	56.364	55	22	1	12	66	101	153	5.18e-13	64.3
MsaG020459	AT4G24400.1	39.286	84	46	2	7	87	95	176	5.70e-13	64.3
MsaG020459	AT1G18040.1	50.909	55	27	0	12	66	100	154	8.13e-13	63.5
MsaG020459	AT1G30270.3	40.000	80	43	1	13	87	24	103	1.23e-12	63.2
MsaG020459	AT1G30270.2	40.000	80	43	1	13	87	121	200	1.30e-12	63.2
MsaG020459	AT1G30270.1	40.000	80	43	1	13	87	121	200	1.31e-12	63.2
MsaG020459	AT5G01810.1	42.647	68	34	1	25	87	113	180	1.46e-12	63.2
MsaG020459	AT5G01810.2	42.647	68	34	1	25	87	113	180	1.46e-12	63.2
MsaG020459	AT4G14580.1	44.118	68	33	1	25	87	124	191	4.39e-12	61.6
MsaG020459	AT5G01810.3	42.647	68	34	1	25	87	113	180	5.41e-12	61.2
MsaG020459	AT5G21326.1	42.667	75	38	1	18	87	108	182	5.42e-12	61.2
MsaG020459	AT1G01140.3	40.260	77	37	2	18	87	114	188	1.21e-11	60.5
MsaG020459	AT1G01140.2	40.260	77	37	2	18	87	114	188	1.28e-11	60.5
MsaG020459	AT1G01140.1	40.260	77	37	2	18	87	114	188	1.29e-11	60.5
MsaG020459	AT2G30360.1	44.928	69	31	2	25	87	124	191	2.44e-11	59.7
MsaG020459	AT5G35410.2	43.939	66	34	1	25	87	113	178	2.71e-11	59.3
MsaG020459	AT5G35410.1	43.939	66	34	1	25	87	113	178	2.97e-11	59.3
MsaG020459	AT2G26980.5	40.000	75	40	1	18	87	109	183	4.22e-11	58.9
MsaG020459	AT2G26980.1	40.000	75	40	1	18	87	109	183	4.26e-11	58.9
MsaG020459	AT2G26980.2	40.000	75	40	1	18	87	109	183	4.30e-11	58.5
MsaG020459	AT2G26980.3	40.000	75	40	1	18	87	109	183	4.42e-11	58.9
MsaG020459	AT1G66750.1	50.000	56	28	0	11	66	100	155	4.55e-11	58.5
MsaG020459	AT2G26980.4	40.000	75	40	1	18	87	119	193	4.61e-11	58.5
MsaG020459	AT5G45820.1	41.176	68	35	1	25	87	113	180	7.04e-11	58.2

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACATACATGTTAAAGATTT+TGG	0.132587	4:-52470044	MsaT020459.1:CDS
GCTTTCCATGGCAGTATATA+AGG	0.207343	4:+52470453	None:intergenic
AAACCAGAGAACCTTCTTAT+TGG	0.231506	4:-52470410	MsaT020459.1:CDS
TTCCTTGTAAAGTTCACCTT+TGG	0.354844	4:+52470760	None:intergenic
CTCATAGACATAAGACTTCA+AGG	0.389555	4:+52470003	None:intergenic
AAACTTAAGCTAGCGGACTT+TGG	0.426034	4:-52470281	MsaT020459.1:CDS
GAGGCAGTATGTTTCATCAT+TGG	0.471154	4:-52470483	MsaT020459.1:CDS
GCACCAATAAGAAGGTTCTC+TGG	0.479972	4:+52470407	None:intergenic
CTTTCCATGGCAGTATATAA+GGG	0.483707	4:+52470454	None:intergenic
TTAAGCTAGCGGACTTTGGA+TGG	0.524617	4:-52470277	MsaT020459.1:CDS
CCTGTGTGCTGCACATGGTC+CGG	0.538815	4:+52470231	None:intergenic
GGATTACCTTCCACCTGGGA+TGG	0.541323	4:-52470210	MsaT020459.1:CDS
ATCCAAAGGTGAACTTTACA+AGG	0.549464	4:-52470762	MsaT020459.1:CDS
TCACTTGTGCACCAATAAGA+AGG	0.556527	4:+52470399	None:intergenic
TGCATACATTCAATCGCAGC+CGG	0.563890	4:-52470250	MsaT020459.1:CDS
ACACAGGATTACCTTCCACC+TGG	0.582633	4:-52470215	MsaT020459.1:CDS
TGTGTATGACATGCTTTCCA+TGG	0.585656	4:+52470441	None:intergenic
GATTACCTTCCACCTGGGAT+GGG	0.593669	4:-52470209	MsaT020459.1:intron
CGTGCCCTTATATACTGCCA+TGG	0.608317	4:-52470458	MsaT020459.1:CDS
CCGGACCATGTGCAGCACAC+AGG	0.619760	4:-52470231	MsaT020459.1:CDS
ACATACCCATCCCAGGTGGA+AGG	0.625505	4:+52470204	None:intergenic
ATGGAAAGCATGTCATACAC+AGG	0.627089	4:-52470439	MsaT020459.1:CDS
GTAATCCTGTGTGCTGCACA+TGG	0.630634	4:+52470226	None:intergenic
CAACTGAACATACCCATCCC+AGG	0.631602	4:+52470197	None:intergenic
CACAGGATTACCTTCCACCT+GGG	0.648638	4:-52470214	MsaT020459.1:CDS
TCAGGGTAAACTTAAGCTAG+CGG	0.660725	4:-52470288	MsaT020459.1:intron
TGGAAAGCATGTCATACACA+GGG	0.705400	4:-52470438	MsaT020459.1:CDS
CTGAACATACCCATCCCAGG+TGG	0.731337	4:+52470200	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	52470004	52470788	52470004	ID=MsaG020459
Chr4	mRNA	52470004	52470788	52470004	ID=MsaT020459.1;Parent=MsaG020459
Chr4	exon	52470004	52470086	52470004	ID=MsaT020459.1.exon4;Parent=MsaT020459.1
Chr4	CDS	52470004	52470086	52470004	ID=cds.MsaT020459.1;Parent=MsaT020459.1
Chr4	exon	52470210	52470306	52470210	ID=MsaT020459.1.exon3;Parent=MsaT020459.1
Chr4	CDS	52470210	52470306	52470210	ID=cds.MsaT020459.1;Parent=MsaT020459.1
Chr4	exon	52470403	52470502	52470403	ID=MsaT020459.1.exon2;Parent=MsaT020459.1
Chr4	CDS	52470403	52470502	52470403	ID=cds.MsaT020459.1;Parent=MsaT020459.1
Chr4	exon	52470718	52470788	52470718	ID=MsaT020459.1.exon1;Parent=MsaT020459.1
Chr4	CDS	52470718	52470788	52470718	ID=cds.MsaT020459.1;Parent=MsaT020459.1

Gene Sequence	>MsaT020459.1 ATGCATCCAAAGGTGAACTTTACAAGGAATTGCAGAAATGCTAATATTTCAGTGAAAGACGTGCAGCTACTGCAGTATGTTTCATCATTGGCTCGTGCCCTTATATACTGCCATGGAAAGCATGTCATACACAGGGACATTAAACCAGAGAACCTTCTTATTGGTGCACAAGGTAAACTTAAGCTAGCGGACTTTGGATGGTCAGTGCATACATTCAATCGCAGCCGGACCATGTGCAGCACACAGGATTACCTTCCACCTGGGATGGGTGTTAATGTTAAGTTCAGCTACATACATGTTAAAGATTTTGGTAACCAAAGAAAAGAACACCTTGAAGTCTTATGTCTATGA
Protein sequence	>MsaT020459.1 MHPKVNFTRNCRNANISVKDVQLLQYVSSLARALIYCHGKHVIHRDIKPENLLIGAQGKLKLADFGWSVHTFNRSRTMCSTQDYLPPGMGVNVKFSYIHVKDFGNQRKEHLEVLCL*