AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020553


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020553 MtrunA17_Chr4g0028071 96.037 328 9 2 1 327 11 335 0.0 639
MsaG020553 MtrunA17_Chr2g0320291 63.043 322 93 9 13 325 9 313 2.95e-127 366
MsaG020553 MtrunA17_Chr8g0365711 31.522 184 119 5 132 311 235 415 1.25e-19 89.0
MsaG020553 MtrunA17_Chr3g0131691 32.544 169 107 2 94 258 247 412 4.30e-17 81.6
MsaG020553 MtrunA17_Chr1g0211081 33.333 144 69 4 136 252 312 455 4.94e-17 81.6
MsaG020553 MtrunA17_Chr8g0366751 29.885 174 108 5 134 294 452 624 7.63e-17 81.3
MsaG020553 MtrunA17_Chr2g0298751 32.099 162 97 4 140 289 51 211 1.14e-16 78.2
MsaG020553 MtrunA17_Chr5g0411341 31.333 150 92 4 145 284 497 645 1.02e-15 78.2
MsaG020553 MtrunA17_Chr4g0048851 32.278 158 90 5 140 292 46 191 5.36e-15 72.8
MsaG020553 MtrunA17_Chr7g0252041 51.471 68 30 1 140 207 285 349 4.55e-14 72.8
MsaG020553 MtrunA17_Chr7g0275821 30.769 156 96 5 145 300 256 399 9.58e-14 71.6
MsaG020553 MtrunA17_Chr8g0346161 27.586 203 110 7 103 284 247 433 7.96e-13 68.9
MsaG020553 MtrunA17_Chr7g0260841 28.814 177 95 6 134 307 345 493 4.21e-12 66.6
MsaG020553 MtrunA17_Chr8g0346151 36.458 96 55 1 105 194 237 332 4.30e-12 66.6
MsaG020553 MtrunA17_Chr4g0054571 27.820 133 90 2 143 269 152 284 1.51e-11 64.3
MsaG020553 MtrunA17_Chr5g0400671 29.747 158 90 5 143 296 156 296 5.39e-11 62.8
MsaG020553 MtrunA17_Chr2g0280161 26.761 142 100 3 130 269 166 305 7.42e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020553 AT2G28160.1 51.976 329 130 11 7 325 7 317 8.72e-95 284
MsaG020553 AT2G28160.2 51.084 323 130 11 7 319 7 311 1.32e-90 273
MsaG020553 AT5G57150.2 39.490 157 85 4 140 288 54 208 1.77e-23 97.1
MsaG020553 AT5G57150.5 39.490 157 85 4 140 288 53 207 1.95e-23 96.7
MsaG020553 AT5G57150.3 39.490 157 85 4 140 288 54 208 2.02e-23 96.7
MsaG020553 AT5G57150.1 39.490 157 85 4 140 288 53 207 2.88e-23 97.1
MsaG020553 AT2G16910.1 35.838 173 85 4 139 286 312 483 5.88e-23 99.8
MsaG020553 AT5G57150.4 39.490 157 85 4 140 288 54 208 7.28e-23 96.3
MsaG020553 AT4G29930.6 36.364 143 82 3 140 281 53 187 1.31e-21 92.0
MsaG020553 AT4G29930.5 34.839 155 91 3 140 294 53 197 3.51e-21 90.9
MsaG020553 AT4G29930.2 35.915 142 81 3 140 281 53 184 1.20e-20 89.4
MsaG020553 AT4G29930.3 34.066 182 85 5 140 294 53 226 6.29e-20 88.2
MsaG020553 AT4G29930.7 34.066 182 85 5 140 294 53 226 6.29e-20 88.2
MsaG020553 AT4G29930.1 34.911 169 75 5 140 281 53 213 1.98e-19 86.7
MsaG020553 AT4G29930.4 36.691 139 66 4 110 237 27 154 8.44e-18 80.5
MsaG020553 AT2G46510.1 38.462 104 59 2 134 234 385 486 3.04e-15 77.0
MsaG020553 AT1G01260.1 32.749 171 83 5 128 290 412 558 5.11e-15 76.3
MsaG020553 AT1G01260.2 32.749 171 83 5 128 290 412 558 5.11e-15 76.3
MsaG020553 AT1G01260.3 32.749 171 83 5 128 290 412 558 5.11e-15 76.3
MsaG020553 AT5G46760.1 27.778 144 98 3 145 284 419 560 6.21e-15 75.9
MsaG020553 AT1G10610.3 34.167 120 76 2 135 251 161 280 9.71e-15 74.3
MsaG020553 AT1G10610.2 34.167 120 76 2 135 251 228 347 1.20e-14 74.7
MsaG020553 AT1G10610.1 34.167 120 76 2 135 251 258 377 1.53e-14 74.3
MsaG020553 AT4G17880.1 29.221 154 104 3 134 284 406 557 4.56e-14 73.2
MsaG020553 AT1G12860.2 40.964 83 46 1 140 222 266 345 1.02e-13 72.0
MsaG020553 AT1G12860.1 40.964 83 46 1 140 222 266 345 1.19e-13 71.6
MsaG020553 AT3G26744.6 50.000 68 31 1 140 207 306 370 1.36e-13 71.6
MsaG020553 AT3G26744.1 50.000 68 31 1 140 207 306 370 1.40e-13 71.6
MsaG020553 AT3G26744.4 50.000 68 31 1 140 207 306 370 1.40e-13 71.6
MsaG020553 AT3G26744.2 50.000 68 31 1 140 207 306 370 1.40e-13 71.6
MsaG020553 AT4G21330.1 27.545 167 118 2 132 296 23 188 2.86e-13 68.2
MsaG020553 AT1G32640.1 29.240 171 83 6 134 284 442 594 3.05e-13 70.9
MsaG020553 AT5G65640.2 44.792 96 49 2 109 200 142 237 6.87e-12 65.5
MsaG020553 AT5G65640.1 45.652 92 46 2 109 196 142 233 9.05e-12 65.9
MsaG020553 AT5G10570.1 33.939 165 89 5 107 269 118 264 9.97e-12 65.5
MsaG020553 AT5G46830.1 28.025 157 95 4 145 295 347 491 2.29e-11 65.1

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGTTCAGAATTCAAATT+TGG 0.148271 4:-54339811 MsaT020553.1:CDS
AAATTTGGCAGGTTGATTTC+TGG 0.184628 4:+54339445 None:intergenic
AACCTCAGCCTTAAGCTTCT+TGG 0.225200 4:+54340167 None:intergenic
CAAAACTTTGATTCATTATA+AGG 0.232350 4:+54340941 None:intergenic
ATCACCCATAGCTTTAAATT+TGG 0.237169 4:+54339430 None:intergenic
AATTGATCCTTGATGAGTTT+TGG 0.238548 4:+54340116 None:intergenic
AAGCTTAAGGCTGAGGTTGC+AGG 0.310327 4:-54340162 MsaT020553.1:CDS
TTGATGAATTGATCAAAGTT+TGG 0.322425 4:+54341018 None:intergenic
TGTTAAGATAGTATGCAATA+AGG 0.332053 4:-54339886 MsaT020553.1:CDS
TTAATGTTTGCTAGTGTCTC+TGG 0.366559 4:+54341075 None:intergenic
AAGTGTACCTTAGTTATATT+CGG 0.367404 4:+54340595 None:intergenic
TTATCTATGTTGTGATCAAA+TGG 0.384556 4:+54340886 None:intergenic
GCTTTACGCATTGCGTTCAT+TGG 0.387836 4:-54340620 MsaT020553.1:CDS
ACCTGCCAAATTTAAAGCTA+TGG 0.396521 4:-54339435 MsaT020553.1:CDS
CGTAAAGCTTATCCTTCATT+CGG 0.412112 4:+54340635 None:intergenic
ATGGATAAGGCTTCTATCAT+TGG 0.423797 4:-54340228 MsaT020553.1:CDS
CCCATAGCTTTAAATTTGGC+AGG 0.425488 4:+54339434 None:intergenic
AATATATGTATAGATGGATA+AGG 0.443648 4:-54340241 MsaT020553.1:intron
CAATTCATCAATTTGATTCG+TGG 0.448543 4:-54341006 MsaT020553.1:CDS
AATTCATCAATTTGATTCGT+GGG 0.449395 4:-54341005 MsaT020553.1:CDS
TTTGATCCTACTTCTTCACT+TGG 0.449750 4:-54340850 MsaT020553.1:CDS
AAGGTATGAATTCAAAGCCT+TGG 0.451801 4:+54339392 None:intergenic
GTTAAGATAGTATGCAATAA+GGG 0.452479 4:-54339885 MsaT020553.1:CDS
TAGTATGCAATAAGGGAGAA+AGG 0.454080 4:-54339878 MsaT020553.1:CDS
GGACTTGAAGCATCATTAGC+AGG 0.454546 4:-54340141 MsaT020553.1:CDS
AATATGCAAGAAAATTGTCC+AGG 0.466755 4:-54340052 MsaT020553.1:intron
CTTTGGTTTCTGAGAGGAGG+AGG 0.467532 4:-54340663 MsaT020553.1:CDS
TGAAAATAGGTGAAAGACTC+TGG 0.467988 4:-54339469 MsaT020553.1:intron
AAATGATAGATCCAAGACTT+TGG 0.473297 4:-54340680 MsaT020553.1:CDS
ATTGGTTCCGAATATAACTA+AGG 0.477975 4:-54340602 MsaT020553.1:intron
AGTATGCAATAAGGGAGAAA+GGG 0.481868 4:-54339877 MsaT020553.1:CDS
TATCTATGTTGTGATCAAAT+GGG 0.486497 4:+54340887 None:intergenic
CCTCTCAGAAACCAAAGTCT+TGG 0.490550 4:+54340669 None:intergenic
TTGTGATCAAATGGGTTTGA+AGG 0.490633 4:+54340895 None:intergenic
ATCACAAGCCAAGAAGCTTA+AGG 0.495865 4:-54340175 MsaT020553.1:CDS
CATCAATTTGATTCGTGGGG+AGG 0.498663 4:-54341001 MsaT020553.1:CDS
TGTTGTTGTTGTTGCAGGAG+AGG 0.508144 4:+54340759 None:intergenic
ATGTGTCAAGCGTGAAAAGA+AGG 0.518768 4:+54339373 None:intergenic
ATGTTTCAAGTGGATGAAAG+AGG 0.519776 4:-54339915 MsaT020553.1:CDS
CCTGCCAAATTTAAAGCTAT+GGG 0.525800 4:-54339434 MsaT020553.1:CDS
AAAGAACCAAGTGAAGAAGT+AGG 0.527298 4:+54340844 None:intergenic
AATTTGGCAACTGTTTCTGA+TGG 0.530693 4:-54339795 MsaT020553.1:CDS
GAGGAGGCGAAGCCGAATGA+AGG 0.530731 4:-54340647 MsaT020553.1:CDS
TGTTTCAAGTGGATGAAAGA+GGG 0.533128 4:-54339914 MsaT020553.1:CDS
ATCACCACTTGTAGTTGTTG+TGG 0.544215 4:+54340720 None:intergenic
AGCCAAGAAGCTTAAGGCTG+AGG 0.549244 4:-54340169 MsaT020553.1:CDS
AGACTTTGGTTTCTGAGAGG+AGG 0.567716 4:-54340666 MsaT020553.1:CDS
GTAGATTGATATGTTTCAAG+TGG 0.578490 4:-54339925 MsaT020553.1:intron
ATTCAATTTACTAACAACAA+TGG 0.579590 4:-54340078 MsaT020553.1:CDS
GCAGGGTCCAAAACTCATCA+AGG 0.584365 4:-54340123 MsaT020553.1:CDS
ATGGGTTTGAAGGAAATGAA+AGG 0.592796 4:+54340905 None:intergenic
CCAAGACTTTGGTTTCTGAG+AGG 0.603254 4:-54340669 MsaT020553.1:CDS
GAATTAAGAGTAGAAAATGA+TGG 0.612562 4:-54340793 MsaT020553.1:CDS
GACTTGAAGCATCATTAGCA+GGG 0.620551 4:-54340140 MsaT020553.1:CDS
ATTACCACAACAACTACAAG+TGG 0.626061 4:-54340724 MsaT020553.1:CDS
ATTCATCAATTTGATTCGTG+GGG 0.631333 4:-54341004 MsaT020553.1:CDS
ATGCAATAAGGGAGAAAGGG+TGG 0.651873 4:-54339874 MsaT020553.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 54339378 54341109 54339378 ID=MsaG020553
Chr4 mRNA 54339378 54341109 54339378 ID=MsaT020553.1;Parent=MsaG020553
Chr4 exon 54339378 54339482 54339378 ID=MsaT020553.1.exon4;Parent=MsaT020553.1
Chr4 CDS 54339378 54339482 54339378 ID=cds.MsaT020553.1;Parent=MsaT020553.1
Chr4 exon 54339770 54339943 54339770 ID=MsaT020553.1.exon3;Parent=MsaT020553.1
Chr4 CDS 54339770 54339943 54339770 ID=cds.MsaT020553.1;Parent=MsaT020553.1
Chr4 exon 54340053 54340250 54340053 ID=MsaT020553.1.exon2;Parent=MsaT020553.1
Chr4 CDS 54340053 54340250 54340053 ID=cds.MsaT020553.1;Parent=MsaT020553.1
Chr4 exon 54340603 54341109 54340603 ID=MsaT020553.1.exon1;Parent=MsaT020553.1
Chr4 CDS 54340603 54341109 54340603 ID=cds.MsaT020553.1;Parent=MsaT020553.1
Gene Sequence

>MsaT020553.1

ATGGATTTTTATCCAGAGACACTAGCAAACATTAACAATGACTATGAGTTACACGATTTCATTGATTATCCAAACTTTGATCAATTCATCAATTTGATTCGTGGGGAGGAGAATGAAGATACTACTATTTGTAACTTCAATTCTGACCTTATAATGAATCAAAGTTTTGTTGATAACTCACTCCTTTCATTTCCTTCAAACCCATTTGATCACAACATAGATAATACTGTCACTGCTTTTGATCCTACTTCTTCACTTGGTTCTTTCTCTTGTTTTGATGGAGAAGCTAAGGGGGAATTAAGAGTAGAAAATGATGGTGATAATTACTCCTCTCCTGCAACAACAACAACAACAACAACAAGCATTACCACAACAACTACAAGTGGTGATACTAAGCCAAAGTCGAAAAATGATAGATCCAAGACTTTGGTTTCTGAGAGGAGGAGGCGAAGCCGAATGAAGGATAAGCTTTACGCATTGCGTTCATTGGTTCCGAATATAACTAAGATGGATAAGGCTTCTATCATTGGAGATGCTGTTTCATATATGCATGAACTTCAATCACAAGCCAAGAAGCTTAAGGCTGAGGTTGCAGGACTTGAAGCATCATTAGCAGGGTCCAAAACTCATCAAGGATCAATTGAAAACCCCAAAAAGATTCAATTTACTAACAACAATGGTTCAATATGCAAGAAAATTGTCCAGATTGATATGTTTCAAGTGGATGAAAGAGGGTTTTATGTTAAGATAGTATGCAATAAGGGAGAAAGGGTGGCTTCTTCATTATACAAGTCTCTTGAATCTTTGAGAGATTTCAATGTTCAGAATTCAAATTTGGCAACTGTTTCTGATGGTTTTCTATTTACATTTTCATTGAATGTGAAAGACTCTGGACCAGAAATCAACCTGCCAAATTTAAAGCTATGGGTGATTAGTGCTTTTCTAAACCAAGGCTTTGAATTCATACCTTCTTTTCACGCTTGA

Protein sequence

>MsaT020553.1

MDFYPETLANINNDYELHDFIDYPNFDQFINLIRGEENEDTTICNFNSDLIMNQSFVDNSLLSFPSNPFDHNIDNTVTAFDPTSSLGSFSCFDGEAKGELRVENDGDNYSSPATTTTTTTSITTTTTSGDTKPKSKNDRSKTLVSERRRRSRMKDKLYALRSLVPNITKMDKASIIGDAVSYMHELQSQAKKLKAEVAGLEASLAGSKTHQGSIENPKKIQFTNNNGSICKKIVQIDMFQVDERGFYVKIVCNKGERVASSLYKSLESLRDFNVQNSNLATVSDGFLFTFSLNVKDSGPEINLPNLKLWVISAFLNQGFEFIPSFHA*