Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020554 | AES76827.2 | 51.142 | 219 | 78 | 8 | 4 | 199 | 97 | 309 | 1.72e-58 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020554 | tr|G7KPD8|G7KPD8_MEDTR | 51.142 | 219 | 78 | 8 | 4 | 199 | 97 | 309 | 8.24e-59 | 196 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG020554 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020554 | MtrunA17_Chr6g0485871 | 51.142 | 219 | 78 | 8 | 4 | 199 | 97 | 309 | 1.59e-62 | 196 |
| MsaG020554 | MtrunA17_Chr7g0259251 | 52.055 | 73 | 29 | 2 | 99 | 167 | 1 | 71 | 9.43e-20 | 79.7 |
| MsaG020554 | MtrunA17_Chr3g0125741 | 65.217 | 46 | 16 | 0 | 7 | 52 | 29 | 74 | 1.51e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCATTAGATTCTTCTGATT+TGG | 0.203292 | 4:-54350787 | MsaT020554.1:CDS |
| CATGATCAATTCATTTATAA+AGG | 0.248719 | 4:-54350537 | MsaT020554.1:CDS |
| CCATGCTTGGTCAAGAATTC+TGG | 0.256325 | 4:-54350570 | MsaT020554.1:CDS |
| GAATCTAATGGAACTATAAA+AGG | 0.283886 | 4:+54350798 | None:intergenic |
| CCATTAGATTCTTCTGATTT+GGG | 0.303530 | 4:-54350786 | MsaT020554.1:CDS |
| CTGTAGCGTTTATGTTCCTC+TGG | 0.347837 | 4:+54351002 | None:intergenic |
| ATGCTACTAATTTGAATACT+TGG | 0.354531 | 4:-54350719 | MsaT020554.1:CDS |
| AGACGATGAGAGTTGTCATT+TGG | 0.365480 | 4:+54350885 | None:intergenic |
| CCCAAATCAGAAGAATCTAA+TGG | 0.410187 | 4:+54350786 | None:intergenic |
| AGCATGGATGAGCGCCACTA+TGG | 0.412834 | 4:-54350855 | MsaT020554.1:CDS |
| TGTTAGATCGACAAGAGTTA+AGG | 0.431807 | 4:-54350502 | MsaT020554.1:CDS |
| AACCTCCTTGAAATACTCCT+TGG | 0.433670 | 4:+54350657 | None:intergenic |
| GACGATGAGAGTTGTCATTT+GGG | 0.440001 | 4:+54350886 | None:intergenic |
| TTTCATTAAAGTCATTGATC+GGG | 0.458302 | 4:-54350610 | MsaT020554.1:CDS |
| ACTCCTTGGGAAATTCAGTA+TGG | 0.473075 | 4:+54350671 | None:intergenic |
| ATCAATTCATTTATAAAGGA+TGG | 0.474045 | 4:-54350533 | MsaT020554.1:CDS |
| ACCTCCTTGAAATACTCCTT+GGG | 0.474430 | 4:+54350658 | None:intergenic |
| AAAGTCATTGATCGGGAGTC+CGG | 0.485891 | 4:-54350603 | MsaT020554.1:CDS |
| ATTTCCCAAGGAGTATTTCA+AGG | 0.485980 | 4:-54350662 | MsaT020554.1:CDS |
| CATCATTCCGTTCTTTGCCA+TGG | 0.495906 | 4:+54350458 | None:intergenic |
| CCAGAATTCTTGACCAAGCA+TGG | 0.497468 | 4:+54350570 | None:intergenic |
| GTTTCATTAAAGTCATTGAT+CGG | 0.502036 | 4:-54350611 | MsaT020554.1:CDS |
| CTACAGGTTCAATCTGTTCA+AGG | 0.503789 | 4:-54350985 | MsaT020554.1:CDS |
| TGGGAAATTCAGTATGGTCA+TGG | 0.514882 | 4:+54350677 | None:intergenic |
| ATGGAACTATAAAAGGTTGT+CGG | 0.519300 | 4:+54350805 | None:intergenic |
| TGTAGCGTTTATGTTCCTCT+GGG | 0.521857 | 4:+54351003 | None:intergenic |
| CATCGAGAAAATGCAGAGAA+AGG | 0.527404 | 4:+54350928 | None:intergenic |
| GCAATGTATCTGCACAAGAT+TGG | 0.541189 | 4:-54350831 | MsaT020554.1:CDS |
| CAGAGGAACATAAACGCTAC+AGG | 0.546873 | 4:-54351001 | MsaT020554.1:CDS |
| ATCTTCACCATGGCAAAGAA+CGG | 0.576109 | 4:-54350465 | MsaT020554.1:CDS |
| TAGATCGACAAGAGTTAAGG+AGG | 0.588263 | 4:-54350499 | MsaT020554.1:CDS |
| TTTGAATACTTGGAAAACGA+AGG | 0.589603 | 4:-54350709 | MsaT020554.1:CDS |
| AATGACAACTCTCATCGTCT+AGG | 0.594641 | 4:-54350882 | MsaT020554.1:CDS |
| TCAATGACTTTAATGAAACA+AGG | 0.613809 | 4:+54350615 | None:intergenic |
| GCAGATACATTGCGCCATAG+TGG | 0.616062 | 4:+54350841 | None:intergenic |
| CAAGCATGGAAAATGTGTAC+CGG | 0.629661 | 4:+54350584 | None:intergenic |
| AAAGAACGGAATGATGATGT+TGG | 0.656487 | 4:-54350451 | MsaT020554.1:CDS |
| TCATCGTCTAGGCTGCAGCA+TGG | 0.662339 | 4:-54350871 | MsaT020554.1:CDS |
| TGACCATACTGAATTTCCCA+AGG | 0.667492 | 4:-54350674 | MsaT020554.1:CDS |
| TCCCAAGGAGTATTTCAAGG+AGG | 0.692766 | 4:-54350659 | MsaT020554.1:CDS |
| AGATCGACAAGAGTTAAGGA+GGG | 0.692933 | 4:-54350498 | MsaT020554.1:CDS |
| TGATAGTCTGATCTTCACCA+TGG | 0.702037 | 4:-54350475 | MsaT020554.1:CDS |
| GTAGCGTTTATGTTCCTCTG+GGG | 0.719905 | 4:+54351004 | None:intergenic |
| TATGGCAAAACACTCCCCAG+AGG | 0.755220 | 4:-54351018 | MsaT020554.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 54350428 | 54351039 | 54350428 | ID=MsaG020554 |
| Chr4 | mRNA | 54350428 | 54351039 | 54350428 | ID=MsaT020554.1;Parent=MsaG020554 |
| Chr4 | exon | 54350428 | 54351039 | 54350428 | ID=MsaT020554.1.exon1;Parent=MsaT020554.1 |
| Chr4 | CDS | 54350428 | 54351039 | 54350428 | ID=cds.MsaT020554.1;Parent=MsaT020554.1 |
| Gene Sequence |
| Protein sequence |
