AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020636

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020636 MtrunA17_Chr4g0028971 98.997 698 6 1 1 697 1 698 0.0 1429
MsaG020636 MtrunA17_Chr2g0326761 71.034 725 156 20 1 697 1 699 0.0 979
MsaG020636 MtrunA17_Chr1g0181211 58.874 693 219 15 9 664 24 687 0.0 763
MsaG020636 MtrunA17_Chr1g0199681 59.201 576 201 16 1 554 83 646 0.0 597
MsaG020636 MtrunA17_Chr7g0264171 55.453 541 214 12 9 536 28 554 0.0 571
MsaG020636 MtrunA17_Chr3g0115271 42.549 557 295 13 9 558 16 554 4.35e-123 379
MsaG020636 MtrunA17_Chr7g0264161 53.623 345 112 6 21 361 1 301 9.33e-119 357
MsaG020636 MtrunA17_Chr5g0424931 40.871 482 222 14 12 474 19 456 1.75e-98 313
MsaG020636 MtrunA17_Chr5g0417281 37.422 481 213 12 4 458 3 421 1.71e-86 281
MsaG020636 MtrunA17_Chr5g0436761 38.839 448 190 12 1 429 1 383 2.28e-83 271
MsaG020636 MtrunA17_Chr2g0285461 38.926 447 205 10 2 436 13 403 4.27e-83 281
MsaG020636 MtrunA17_Chr5g0433301 39.186 393 186 7 5 388 17 365 1.86e-79 270
MsaG020636 MtrunA17_Chr2g0289221 40.541 370 138 9 9 361 17 321 1.48e-77 252
MsaG020636 MtrunA17_Chr1g0156321 40.731 383 171 8 9 381 40 376 1.82e-77 265
MsaG020636 MtrunA17_Chr2g0277461 35.463 454 211 11 3 447 4 384 4.13e-77 254
MsaG020636 MtrunA17_Chr4g0046371 40.104 384 171 9 12 381 38 376 6.87e-77 260
MsaG020636 MtrunA17_Chr8g0385791 38.847 399 185 7 12 399 59 409 1.73e-76 262
MsaG020636 MtrunA17_Chr8g0376771 37.753 445 212 12 2 436 13 402 2.73e-76 263
MsaG020636 MtrunA17_Chr3g0109391 37.740 416 204 8 6 412 20 389 3.86e-76 261
MsaG020636 MtrunA17_Chr1g0178011 36.946 406 186 10 9 401 9 357 6.08e-73 243
MsaG020636 MtrunA17_Chr8g0346711 31.329 715 360 26 3 670 16 646 2.71e-72 247
MsaG020636 MtrunA17_Chr7g0239061 29.231 715 386 24 1 662 3 650 3.22e-72 247
MsaG020636 MtrunA17_Chr4g0070181 38.015 413 193 10 3 404 14 374 4.86e-72 253
MsaG020636 MtrunA17_Chr5g0417211 37.563 394 170 8 9 391 9 337 7.17e-72 239
MsaG020636 MtrunA17_Chr2g0301251 38.010 392 184 7 3 383 14 357 2.58e-71 251
MsaG020636 MtrunA17_Chr4g0029671 37.956 411 187 6 12 402 54 416 3.84e-71 246
MsaG020636 MtrunA17_Chr2g0276781 36.989 465 203 14 12 440 23 433 2.20e-70 242
MsaG020636 MtrunA17_Chr2g0326281 37.123 431 192 10 12 410 56 439 1.11e-69 243
MsaG020636 MtrunA17_Chr8g0389081 36.735 392 195 7 2 384 22 369 1.90e-63 228
MsaG020636 MtrunA17_Chr2g0282961 48.826 213 100 2 6 209 47 259 9.02e-58 206
MsaG020636 MtrunA17_Chr2g0282961 37.963 108 66 1 274 381 281 387 1.90e-18 89.7
MsaG020636 MtrunA17_Chr5g0425261 35.930 398 170 14 9 387 9 340 8.42e-57 197
MsaG020636 MtrunA17_Chr5g0424801 35.969 392 169 14 9 382 9 336 4.58e-56 196
MsaG020636 MtrunA17_Chr4g0009751 49.528 212 99 4 7 211 14 224 2.34e-53 194
MsaG020636 MtrunA17_Chr5g0424741 31.628 430 202 14 9 398 9 386 3.40e-48 177
MsaG020636 MtrunA17_Chr2g0277531 32.162 370 170 13 7 361 15 318 6.40e-48 171
MsaG020636 MtrunA17_Chr5g0425661 43.367 196 93 3 12 196 37 225 5.21e-47 166
MsaG020636 MtrunA17_Chr8g0356211 30.619 307 155 7 116 422 2 250 3.04e-38 144
MsaG020636 MtrunA17_Chr5g0425651 43.220 118 60 3 275 388 1 115 1.84e-21 90.5
MsaG020636 MtrunA17_Chr5g0424791 32.432 148 89 4 58 204 14 151 7.43e-16 75.5
MsaG020636 MtrunA17_Chr1g0178071 32.432 148 83 4 103 238 71 213 4.04e-11 65.1
MsaG020636 MtrunA17_Chr8g0345771 39.000 100 59 2 103 202 167 264 8.39e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020636 AT2G28350.1 57.838 740 219 21 3 697 2 693 0.0 753
MsaG020636 AT4G30080.1 56.023 689 248 14 4 669 12 668 0.0 716
MsaG020636 AT2G28350.2 57.363 713 211 21 3 670 2 666 0.0 714
MsaG020636 AT1G77850.1 42.918 473 233 12 9 467 16 465 2.20e-116 362
MsaG020636 AT1G77850.2 42.918 473 233 12 9 467 16 465 3.43e-116 362
MsaG020636 AT1G59750.4 31.930 689 339 20 12 662 22 618 1.10e-81 273
MsaG020636 AT1G59750.2 32.122 688 341 20 12 662 22 620 1.65e-81 272
MsaG020636 AT1G59750.1 32.075 689 343 20 12 662 22 623 5.23e-81 271
MsaG020636 AT1G59750.3 32.075 689 343 20 12 662 22 623 5.23e-81 271
MsaG020636 AT1G30330.1 40.097 414 193 8 3 407 14 381 7.74e-81 276
MsaG020636 AT1G30330.3 40.049 412 192 8 5 407 18 383 2.76e-80 275
MsaG020636 AT1G30330.2 40.049 412 192 8 5 407 18 383 2.76e-80 275
MsaG020636 AT5G37020.2 38.817 389 185 7 9 388 19 363 4.26e-79 268
MsaG020636 AT5G37020.1 38.817 389 185 7 9 388 19 363 1.17e-78 268
MsaG020636 AT2G33860.1 33.533 501 245 13 12 474 54 504 5.54e-76 256
MsaG020636 AT5G60450.2 38.670 406 184 9 12 400 41 398 1.96e-75 258
MsaG020636 AT5G60450.1 38.670 406 184 9 12 400 65 422 3.00e-75 258
MsaG020636 AT5G62000.2 39.409 406 185 10 9 400 52 410 6.33e-74 256
MsaG020636 AT5G62000.5 39.409 406 185 10 9 400 52 410 6.33e-74 256
MsaG020636 AT5G62000.3 39.409 406 185 10 9 400 52 410 6.33e-74 256
MsaG020636 AT5G62000.1 39.409 406 185 10 9 400 52 410 6.33e-74 256
MsaG020636 AT5G62000.4 39.409 406 185 10 9 400 52 410 7.85e-74 255
MsaG020636 AT2G46530.1 39.005 382 169 12 12 381 21 350 5.14e-67 232
MsaG020636 AT2G46530.3 39.005 382 169 12 12 381 42 371 9.54e-67 231
MsaG020636 AT1G19850.1 37.755 392 181 9 4 381 46 388 2.62e-66 235
MsaG020636 AT1G19850.2 37.755 392 181 9 4 381 35 377 2.97e-66 235
MsaG020636 AT3G61830.1 36.131 429 206 13 4 418 14 388 2.97e-65 227
MsaG020636 AT1G34310.1 29.359 671 356 22 6 660 21 589 2.07e-61 216
MsaG020636 AT1G35520.1 30.370 675 344 25 6 660 21 589 4.20e-61 215
MsaG020636 AT1G34390.1 28.739 682 358 22 6 667 21 594 1.74e-59 211
MsaG020636 AT4G23980.2 35.279 394 176 12 8 381 8 342 6.60e-58 207
MsaG020636 AT4G23980.1 35.279 394 176 12 8 381 8 342 7.17e-58 207
MsaG020636 AT1G35240.1 36.364 396 183 14 6 387 21 361 1.14e-57 206
MsaG020636 AT1G19220.1 48.869 221 96 4 3 211 14 229 2.80e-57 211
MsaG020636 AT1G19220.1 40.000 130 67 4 286 412 261 382 2.19e-17 87.4
MsaG020636 AT5G37020.3 38.699 292 134 5 98 388 21 268 9.46e-57 206
MsaG020636 AT1G35540.1 34.663 401 188 13 6 395 21 358 2.21e-56 202
MsaG020636 AT1G34410.1 35.401 387 184 12 6 381 21 352 2.54e-56 202
MsaG020636 AT5G20730.2 47.059 221 100 4 3 211 15 230 9.88e-56 206
MsaG020636 AT5G20730.2 42.478 113 64 1 286 398 262 373 2.77e-18 90.5
MsaG020636 AT5G20730.3 47.059 221 100 4 3 211 15 230 1.02e-55 206
MsaG020636 AT5G20730.3 42.105 114 64 2 286 398 262 374 5.98e-17 85.9
MsaG020636 AT5G20730.1 47.059 221 100 4 3 211 15 230 1.21e-55 206
MsaG020636 AT5G20730.1 42.105 114 64 2 286 398 262 374 5.02e-17 86.3
MsaG020636 AT2G33860.2 32.576 396 188 10 108 474 2 347 3.99e-54 192
MsaG020636 AT3G61830.2 35.946 370 180 10 52 418 22 337 1.43e-52 191
MsaG020636 AT1G34170.1 33.075 387 195 12 6 381 19 352 3.55e-52 189
MsaG020636 AT2G46530.2 38.406 276 121 6 107 381 36 263 3.94e-46 172
MsaG020636 AT1G34170.2 38.636 220 118 5 6 214 19 232 8.10e-42 159
MsaG020636 AT1G34170.3 38.636 220 118 5 6 214 19 232 2.71e-41 159
MsaG020636 AT1G43950.1 39.336 211 110 6 6 207 21 222 8.02e-38 140
MsaG020636 ATMG00940.1 38.037 163 85 5 195 356 1 148 7.12e-22 93.6
MsaG020636 AT2G36080.4 37.838 111 63 3 103 208 33 142 1.75e-11 63.9
MsaG020636 AT2G36080.2 37.838 111 63 3 103 208 33 142 1.75e-11 63.9
MsaG020636 AT2G36080.1 37.838 111 63 3 103 208 33 142 4.12e-11 64.3
MsaG020636 AT2G36080.3 37.838 111 63 3 103 208 33 142 4.15e-11 63.9

Find 162 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAGGATTAGTTGGTTTA+TGG 0.143329 4:+55894941 MsaT020636.1:CDS
TCATCTTGTGGTTGGACTTC+AGG 0.221219 4:+55894609 MsaT020636.1:CDS
TTGAAACTGAAGACTCTTCT+AGG 0.222931 4:+55894925 MsaT020636.1:CDS
GGAAAGTGAGAAAGGGTATT+TGG 0.265159 4:+55893957 MsaT020636.1:CDS
TTGACAATTCTGACAGATTC+AGG 0.268164 4:+55896869 MsaT020636.1:CDS
ACCCTTGACTTATCACTTCT+TGG 0.269886 4:+55896362 MsaT020636.1:CDS
TTCTCCGTGGCGCCTTCTTC+AGG 0.276881 4:+55895010 MsaT020636.1:CDS
CTAATACAAACATTGATCTA+AGG 0.281084 4:+55894076 MsaT020636.1:CDS
AACAATGTAAATTGTGGAAT+TGG 0.287100 4:+55894642 MsaT020636.1:CDS
CAGGCATGATAAGCTTTCTT+TGG 0.290621 4:-55896051 None:intergenic
TGGAATTCGTAGGGCGAAAA+AGG 0.294025 4:+55894563 MsaT020636.1:CDS
GGGCCAAACAGCCCCTTCTT+TGG 0.299415 4:+55895840 MsaT020636.1:CDS
GGAGCCAGGCATGCTCAATT+TGG 0.301173 4:+55895894 MsaT020636.1:CDS
CACCGGCAACAAGCTTCTTC+TGG 0.313373 4:-55894497 None:intergenic
AGAAGGCGCCACGGAGAATT+AGG 0.313389 4:-55895005 None:intergenic
GGGAACAATTGCTTCTGTTC+AGG 0.316761 4:+55894962 MsaT020636.1:CDS
ATGAGGATTCAATGGTGTTC+TGG 0.321716 4:+55894882 MsaT020636.1:CDS
CGCCGGCACTTGCTTACAAC+TGG 0.322681 4:+55894456 MsaT020636.1:CDS
TCTAGGATTAGTTGGTTTAT+GGG 0.340586 4:+55894942 MsaT020636.1:CDS
AATTGTGGAATTGGACCTTT+TGG 0.346313 4:+55894651 MsaT020636.1:CDS
TGTGGTTGGACTTCAGGTTC+AGG 0.346418 4:+55894615 MsaT020636.1:CDS
TGGCAACTTGGATTGGATAT+TGG 0.355146 4:+55896299 MsaT020636.1:CDS
TATCATGCCTGTTGAGTATA+GGG 0.358214 4:+55896063 MsaT020636.1:CDS
TAATGAAGCTTCTTCCTTTG+AGG 0.360496 4:+55894181 MsaT020636.1:CDS
AGCAACAATAAACTTCAGTT+AGG 0.363663 4:+55895945 MsaT020636.1:CDS
ACGGGGAGCTTTGGAAGTTT+AGG 0.363924 4:+55894412 MsaT020636.1:CDS
CTAATACAGCTGAGTTTAGT+TGG 0.368826 4:+55896279 MsaT020636.1:CDS
TTCACCACTGATTCTTTGTA+AGG 0.370999 4:+55894107 MsaT020636.1:CDS
GGTTGTTGGTGTTAAATTCA+TGG 0.380615 4:+55894128 MsaT020636.1:CDS
AGGATGTGCACGGGGAGCTT+TGG 0.383797 4:+55894403 MsaT020636.1:CDS
TGTTATGGAAGCTGTGAAAT+TGG 0.385027 4:+55894770 MsaT020636.1:CDS
AGTGAGGGCTGAAGATGTTA+TGG 0.385830 4:+55894755 MsaT020636.1:CDS
AAACCAAAGAAGGGGCTGTT+TGG 0.394320 4:-55895843 None:intergenic
CAATGCTCCTGCATGTATAC+AGG 0.395010 4:+55895872 MsaT020636.1:CDS
AAGACTCTTCTAGGATTAGT+TGG 0.399400 4:+55894934 MsaT020636.1:CDS
CACTCCCGAGTTCTGTGTTA+AGG 0.400303 4:+55894839 MsaT020636.1:CDS
TTATCATGCCTGTTGAGTAT+AGG 0.406824 4:+55896062 MsaT020636.1:CDS
ACAGGTGCTGTTAAGCAAAT+TGG 0.408215 4:+55896473 MsaT020636.1:CDS
GAGAATAAGACATTGAGAAA+TGG 0.411485 4:+55894699 MsaT020636.1:CDS
TTCTTCCTCGGTGGAGTGAA+AGG 0.420088 4:-55895741 None:intergenic
AGGTGGTGGTGGTGATGGTT+TGG 0.423416 4:+55894227 MsaT020636.1:CDS
TTCGCCACCACCGTCTGAAC+AGG 0.425252 4:-55894381 None:intergenic
CTGATTCTTTGTAAGGTTGT+TGG 0.426252 4:+55894114 MsaT020636.1:CDS
GCCTACTACTACAGGTTACA+TGG 0.430758 4:+55895631 MsaT020636.1:intron
GGATAGTAAACAACTTCAAA+TGG 0.432142 4:-55894807 None:intergenic
TGAGGATTCAATGGTGTTCT+GGG 0.446254 4:+55894883 MsaT020636.1:CDS
TTCAATGGTGTTCTGGGATG+AGG 0.448575 4:+55894889 MsaT020636.1:CDS
TCCTCCAAATTGAGCATGCC+TGG 0.451469 4:-55895898 None:intergenic
ACTGAAGTTTATTGTTGCTA+AGG 0.452992 4:-55895941 None:intergenic
GCTGAGTTTAGTTGGCAACT+TGG 0.454025 4:+55896287 MsaT020636.1:CDS
GATGAAGGTGGTGGTGGTGA+TGG 0.454860 4:+55894222 MsaT020636.1:CDS
CATTATGCAAATATGCTTAG+AGG 0.456170 4:-55897073 None:intergenic
CAGCTATGGCATGCTTGTGC+TGG 0.456859 4:+55893985 MsaT020636.1:CDS
AACTCGGGAGTGCTTGCTCT+TGG 0.457681 4:-55894828 None:intergenic
AGCACCTTGATGTGCATAGT+AGG 0.468204 4:+55895988 MsaT020636.1:CDS
TCAAGGTGCTGAATGTTAGT+AGG 0.470920 4:-55895975 None:intergenic
AAGTTTAGGCATATTTATAG+AGG 0.471597 4:+55894426 MsaT020636.1:CDS
TTGATGGTTCATCTTGTGGT+TGG 0.472441 4:+55894601 MsaT020636.1:CDS
AACCAGAAGAAGCTTGTTGC+CGG 0.474099 4:+55894495 MsaT020636.1:CDS
TCAGAGCTTGGTTATGATGA+AGG 0.480927 4:+55894207 MsaT020636.1:CDS
TTGGATGCATCAAACAAAAC+AGG 0.481900 4:+55897049 MsaT020636.1:CDS
TCAATGAAGGAAAGTGAGAA+AGG 0.482138 4:+55893949 None:intergenic
ATTGTCAGATAAACCAAAGA+AGG 0.484820 4:-55895853 None:intergenic
GGTTATGATGAAGGTGGTGG+TGG 0.486406 4:+55894216 MsaT020636.1:CDS
TGAGTGGAAGAGTGAGAGTG+AGG 0.488015 4:+55894739 MsaT020636.1:CDS
TCACAAAGGTGAACATGGAT+TGG 0.488178 4:+55897030 MsaT020636.1:CDS
GTTTAGTTGGCAACTTGGAT+TGG 0.492268 4:+55896292 MsaT020636.1:CDS
GCAACAATAAACTTCAGTTA+GGG 0.494974 4:+55895946 MsaT020636.1:CDS
AGGGGCTGTTTGGCCCGAGC+TGG 0.496697 4:-55895833 None:intergenic
TGTTCAGACGGTGGTGGCGA+AGG 0.501569 4:+55894383 MsaT020636.1:CDS
GGTTCTAACAATGTAAATTG+TGG 0.502204 4:+55894636 MsaT020636.1:CDS
AGAAGAGTCTTCAGTTTCAA+AGG 0.502379 4:-55894922 None:intergenic
GGAGAATTAGGCCAGCGAAT+AGG 0.504468 4:-55894993 None:intergenic
GGCACTTGCTTACAACTGGT+TGG 0.507228 4:+55894460 MsaT020636.1:CDS
AGGTTGTTGATCCTATTCGC+TGG 0.509848 4:+55894982 MsaT020636.1:CDS
AATCTGATGCAAACAATGGT+GGG 0.510020 4:+55894292 MsaT020636.1:CDS
CTATGGCATGCTTGTGCTGG+TGG 0.512929 4:+55893988 MsaT020636.1:CDS
GCATGCTTGTGCTGGTGGAA+TGG 0.513876 4:+55893993 MsaT020636.1:CDS
AGTAAACAACTTCAAATGGT+TGG 0.519885 4:-55894803 None:intergenic
TTGTGGAAAGTAGAAAACTT+TGG 0.520773 4:-55894037 None:intergenic
TGTTAAGGCTTCAGCTGTGA+AGG 0.522163 4:+55894854 MsaT020636.1:CDS
CCTTTGAGGAATTCAGAGCT+TGG 0.522861 4:+55894195 MsaT020636.1:CDS
GGCTGAGCCGCCTGTTCAGA+CGG 0.523312 4:+55894371 MsaT020636.1:CDS
CTGCATGTATACAGGGAGCC+AGG 0.523472 4:+55895880 MsaT020636.1:CDS
GAAGAGTCTTCAGTTTCAAA+GGG 0.524779 4:-55894921 None:intergenic
GGAAACCGCAGCTTCTTCCT+CGG 0.526125 4:-55895753 None:intergenic
TAATACAAACATTGATCTAA+GGG 0.528805 4:+55894077 MsaT020636.1:CDS
ATTTGTTGCTCAGTGAGTAT+TGG 0.534131 4:-55896167 None:intergenic
TGAAGCCTTAACACAGAACT+CGG 0.535150 4:-55894844 None:intergenic
AAATTGGTGAAGAACCCTTC+AGG 0.536171 4:+55896489 MsaT020636.1:CDS
TCTTCCTCGGTGGAGTGAAA+GGG 0.536181 4:-55895740 None:intergenic
AACCAGTTGTAAGCAAGTGC+CGG 0.536252 4:-55894458 None:intergenic
CAATGAAGGAAAGTGAGAAA+GGG 0.542351 4:+55893950 None:intergenic
AACCAAGAAGTGATAAGTCA+AGG 0.543232 4:-55896364 None:intergenic
TTTATAGAGGGACGCCGCGC+CGG 0.546376 4:+55894439 MsaT020636.1:CDS
TGGTGGCGAAGGATGTGCAC+GGG 0.547205 4:+55894394 MsaT020636.1:CDS
GCATGTTCAGCATGTCCTTG+TGG 0.548555 4:-55894054 None:intergenic
CAGCTGTGAAGGCTGCTATG+AGG 0.549739 4:+55894865 MsaT020636.1:CDS
CTGGCTCCCTGTATACATGC+AGG 0.552767 4:-55895879 None:intergenic
GATACTAATTCGACCAACCA+CGG 0.553761 4:-55895693 None:intergenic
AATTGTGTGTTGGAATTCGT+AGG 0.556594 4:+55894553 MsaT020636.1:CDS
ATCGTCTCAGCACAGTACCT+TGG 0.557673 4:-55894327 None:intergenic
TCTGATGCAAACAATGGTGG+GGG 0.561128 4:+55894294 MsaT020636.1:CDS
GCCGCCTGTTCAGACGGTGG+TGG 0.561486 4:+55894377 MsaT020636.1:CDS
ATACTAATTCGACCAACCAC+GGG 0.564828 4:-55895692 None:intergenic
GCCAGGCATGCTCAATTTGG+AGG 0.565841 4:+55895897 MsaT020636.1:CDS
ATTGGATATTGGTCATTGCA+AGG 0.566046 4:+55896310 MsaT020636.1:CDS
GGAACTCACAAAGGTGAACA+TGG 0.567844 4:+55897025 MsaT020636.1:CDS
GAAGCGTGTTAGCCCGTGGT+TGG 0.567936 4:+55895680 MsaT020636.1:CDS
TTGTCAGATAAACCAAAGAA+GGG 0.569032 4:-55895852 None:intergenic
ATAGTAAAGACAAAGAGAAG+TGG 0.569647 4:+55896225 MsaT020636.1:CDS
CAATCTGATGCAAACAATGG+TGG 0.570087 4:+55894291 MsaT020636.1:CDS
AGTTTAGGCATATTTATAGA+GGG 0.570327 4:+55894427 MsaT020636.1:CDS
ACCAAGAAGTGATAAGTCAA+GGG 0.572563 4:-55896363 None:intergenic
AAAACTTTGGAGTTCACTTG+AGG 0.572950 4:-55894024 None:intergenic
GTGGTGGCGAAGGATGTGCA+CGG 0.577381 4:+55894393 MsaT020636.1:CDS
ATCTGATGCAAACAATGGTG+GGG 0.580572 4:+55894293 MsaT020636.1:CDS
AAAGTCTTAGCAAAAGATGC+AGG 0.580849 4:-55894264 None:intergenic
CCAAGCTCTGAATTCCTCAA+AGG 0.581047 4:-55894195 None:intergenic
AAATCCTACTATGCACATCA+AGG 0.584801 4:-55895992 None:intergenic
GAGAATGGTGAATTGTGTGT+TGG 0.591957 4:+55894543 MsaT020636.1:CDS
GAGTGGAAGAGTGAGAGTGA+GGG 0.596583 4:+55894740 MsaT020636.1:CDS
TGACGGAAATTGAACATCGA+GGG 0.599791 4:-55895793 None:intergenic
CTTTCCGAGGCTTGATTACG+CGG 0.601538 4:+55894350 MsaT020636.1:CDS
TTGCCTGAAAACGTCGGTGA+CGG 0.603464 4:-55895810 None:intergenic
TGGAGTCAGAAGATGTTGGA+AGG 0.603517 4:+55896339 MsaT020636.1:CDS
AGGCTGCTATGAGGATTCAA+TGG 0.603915 4:+55894874 MsaT020636.1:CDS
CTCCACCGAGGAAGAAGCTG+CGG 0.604549 4:+55895748 MsaT020636.1:CDS
GGTATTTGGATCCACAGCTA+TGG 0.606132 4:+55893971 MsaT020636.1:CDS
ACACAAAAGCAATACCTGAA+GGG 0.611431 4:-55896503 None:intergenic
AACCGCAGCTTCTTCCTCGG+TGG 0.613433 4:-55895750 None:intergenic
GCCACCACCGTCTGAACAGG+CGG 0.614156 4:-55894378 None:intergenic
TTCTTGGAGTCAGAAGATGT+TGG 0.615581 4:+55896335 MsaT020636.1:CDS
AAAGGGCTGAAATGAATGAC+TGG 0.619094 4:-55895723 None:intergenic
CCTACTACTACAGGTTACAT+GGG 0.623681 4:+55895632 MsaT020636.1:intron
GCTTGTTGCCGGTGATTCTG+TGG 0.623881 4:+55894506 MsaT020636.1:CDS
CTTGGTTATGATGAAGGTGG+TGG 0.625908 4:+55894213 MsaT020636.1:CDS
GACGGAAATTGAACATCGAG+GGG 0.629218 4:-55895792 None:intergenic
GGTGGCGAAGGATGTGCACG+GGG 0.629593 4:+55894395 MsaT020636.1:CDS
GCACAAGCATGCCATAGCTG+TGG 0.631502 4:-55893982 None:intergenic
GTGCTGAGACGATCTTTCCG+AGG 0.634969 4:+55894337 MsaT020636.1:CDS
TCAGCCGCGTAATCAAGCCT+CGG 0.639890 4:-55894354 None:intergenic
ATTGTGTGTTGGAATTCGTA+GGG 0.641411 4:+55894554 MsaT020636.1:CDS
AATGCTCCTGCATGTATACA+GGG 0.643769 4:+55895873 MsaT020636.1:CDS
GGCTGACCCTGAAACTGATG+AGG 0.645568 4:+55894149 MsaT020636.1:CDS
ACGTGAAGCGTGTTAGCCCG+TGG 0.646883 4:+55895676 MsaT020636.1:CDS
AATACCTGAAGAAGGCGCCA+CGG 0.652216 4:-55895014 None:intergenic
TAACAGCACCTGTTGCATCA+CGG 0.662294 4:-55896463 None:intergenic
TGAGCCGCCTGTTCAGACGG+TGG 0.665475 4:+55894374 MsaT020636.1:CDS
GTTGTAAGCAAGTGCCGGCG+CGG 0.666685 4:-55894453 None:intergenic
GAAGCCTTAACACAGAACTC+GGG 0.673594 4:-55894843 None:intergenic
TGGATAACAGGAACTCACAA+AGG 0.673838 4:+55897016 MsaT020636.1:CDS
TGTCAGATAAACCAAAGAAG+GGG 0.674937 4:-55895851 None:intergenic
AGCTGGTTGCCTGAAAACGT+CGG 0.679058 4:-55895816 None:intergenic
ACAACCTTACAAAGAATCAG+TGG 0.680398 4:-55894111 None:intergenic
TTCGCTGGCCTAATTCTCCG+TGG 0.685289 4:+55894997 MsaT020636.1:CDS
GTGACGGAAATTGAACATCG+AGG 0.688190 4:-55895794 None:intergenic
CTGATGCAAACAATGGTGGG+GGG 0.689649 4:+55894295 MsaT020636.1:CDS
GAGCTTGGTTATGATGAAGG+TGG 0.703479 4:+55894210 MsaT020636.1:CDS
ACTCAATCTGATGCAAACAA+TGG 0.706199 4:+55894288 MsaT020636.1:CDS
ACGGAAATTGAACATCGAGG+GGG 0.745927 4:-55895791 None:intergenic
TCAGCCCTTTCACTCCACCG+AGG 0.764350 4:+55895736 MsaT020636.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 55893952 55897093 55893952 ID=MsaG020636
Chr4 mRNA 55893952 55897093 55893952 ID=MsaT020636.1;Parent=MsaG020636
Chr4 exon 55893952 55895031 55893952 ID=MsaT020636.1.exon1;Parent=MsaT020636.1
Chr4 CDS 55893952 55895031 55893952 ID=cds.MsaT020636.1;Parent=MsaT020636.1
Chr4 exon 55895645 55896510 55895645 ID=MsaT020636.1.exon2;Parent=MsaT020636.1
Chr4 CDS 55895645 55896510 55895645 ID=cds.MsaT020636.1;Parent=MsaT020636.1
Chr4 exon 55896844 55896909 55896844 ID=MsaT020636.1.exon3;Parent=MsaT020636.1
Chr4 CDS 55896844 55896909 55896844 ID=cds.MsaT020636.1;Parent=MsaT020636.1
Chr4 exon 55897012 55897093 55897012 ID=MsaT020636.1.exon4;Parent=MsaT020636.1
Chr4 CDS 55897012 55897093 55897012 ID=cds.MsaT020636.1;Parent=MsaT020636.1
Gene Sequence

>MsaT020636.1

ATGAAGGAAAGTGAGAAAGGGTATTTGGATCCACAGCTATGGCATGCTTGTGCTGGTGGAATGGTTCAGATACCTCAAGTGAACTCCAAAGTTTTCTACTTTCCACAAGGACATGCTGAACATGCTAATACAAACATTGATCTAAGGGTTTCAGTTTCACCACTGATTCTTTGTAAGGTTGTTGGTGTTAAATTCATGGCTGACCCTGAAACTGATGAGGTTTTTGCTGTAATGAAGCTTCTTCCTTTGAGGAATTCAGAGCTTGGTTATGATGAAGGTGGTGGTGGTGATGGTTTGGAAAATTCAGAAAAGCCTGCATCTTTTGCTAAGACTTTGACTCAATCTGATGCAAACAATGGTGGGGGGTTTTCTGTTCCAAGGTACTGTGCTGAGACGATCTTTCCGAGGCTTGATTACGCGGCTGAGCCGCCTGTTCAGACGGTGGTGGCGAAGGATGTGCACGGGGAGCTTTGGAAGTTTAGGCATATTTATAGAGGGACGCCGCGCCGGCACTTGCTTACAACTGGTTGGAGTAGTTTTGTGAACCAGAAGAAGCTTGTTGCCGGTGATTCTGTGGTGTTTTTGAGGGCTGAGAATGGTGAATTGTGTGTTGGAATTCGTAGGGCGAAAAAGGGAATTGGTGGTGGTTTTGATGGTTCATCTTGTGGTTGGACTTCAGGTTCAGGTTCTAACAATGTAAATTGTGGAATTGGACCTTTTGGAGCATTTTCTTTTTTCTTGAAAGAAGAGAATAAGACATTGAGAAATGGTTGTTTTGATGGTAATTTGAGTGGAAGAGTGAGAGTGAGGGCTGAAGATGTTATGGAAGCTGTGAAATTGGCTGCTAATAACCAACCATTTGAAGTTGTTTACTATCCAAGAGCAAGCACTCCCGAGTTCTGTGTTAAGGCTTCAGCTGTGAAGGCTGCTATGAGGATTCAATGGTGTTCTGGGATGAGGTTCAAGATGCCCTTTGAAACTGAAGACTCTTCTAGGATTAGTTGGTTTATGGGAACAATTGCTTCTGTTCAGGTTGTTGATCCTATTCGCTGGCCTAATTCTCCGTGGCGCCTTCTTCAGGTTACATGGGATGAGCCTGATTTACTACAAAACGTGAAGCGTGTTAGCCCGTGGTTGGTCGAATTAGTATCAAATGTACCAGTCATTCATTTCAGCCCTTTCACTCCACCGAGGAAGAAGCTGCGGTTTCCACCAAACCTCGACTTCCCCCTCGATGTTCAATTTCCGTCACCGACGTTTTCAGGCAACCAGCTCGGGCCAAACAGCCCCTTCTTTGGTTTATCTGACAATGCTCCTGCATGTATACAGGGAGCCAGGCATGCTCAATTTGGAGGAATATCTCTTTCAGATTTCAACCTTAGCAACAATAAACTTCAGTTAGGGTTGTTTCCTACTAACATTCAGCACCTTGATGTGCATAGTAGGATTTCAAATAGCAACAACATGATGAACAACAATGATAAGACCAAAGAAAGCTTATCATGCCTGTTGAGTATAGGGAAAAAATATGATAAAAGTTTGGAGAAATCTCAATCTGATGACAATGTTGTTAAGAAACATCAGTTTTGTCTCTTTGGTCAACCAATACTCACTGAGCAACAAATTTCTAGAAACTGTTCAGAAAAAAATTCAGTAGATGATAGTAAAGACAAAGAGAAGTGGTTTTTGGATGCATTTTCAGCTGGGAAAGGTTCTAATACAGCTGAGTTTAGTTGGCAACTTGGATTGGATATTGGTCATTGCAAGGTTTTCTTGGAGTCAGAAGATGTTGGAAGGACCCTTGACTTATCACTTCTTGGTTCTTATGAAGAGCTCTACAAGAAACTTGCTAACATGTTTGATTTAGAAAAGTCTGAGATGTTGAATCATGTTTTTTACCGTGATGCAACAGGTGCTGTTAAGCAAATTGGTGAAGAACCCTTCAGTGAATTCATGAAGACAGCAAAAAGATTGACAATTCTGACAGATTCAGGCAGCAAAAATGTGAGAGGGGCATGGATAACAGGAACTCACAAAGGTGAACATGGATTGGATGCATCAAACAAAACAGGACCTCTAAGCATATTTGCATAA
Protein sequence

>MsaT020636.1

MKESEKGYLDPQLWHACAGGMVQIPQVNSKVFYFPQGHAEHANTNIDLRVSVSPLILCKVVGVKFMADPETDEVFAVMKLLPLRNSELGYDEGGGGDGLENSEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYAAEPPVQTVVAKDVHGELWKFRHIYRGTPRRHLLTTGWSSFVNQKKLVAGDSVVFLRAENGELCVGIRRAKKGIGGGFDGSSCGWTSGSGSNNVNCGIGPFGAFSFFLKEENKTLRNGCFDGNLSGRVRVRAEDVMEAVKLAANNQPFEVVYYPRASTPEFCVKASAVKAAMRIQWCSGMRFKMPFETEDSSRISWFMGTIASVQVVDPIRWPNSPWRLLQVTWDEPDLLQNVKRVSPWLVELVSNVPVIHFSPFTPPRKKLRFPPNLDFPLDVQFPSPTFSGNQLGPNSPFFGLSDNAPACIQGARHAQFGGISLSDFNLSNNKLQLGLFPTNIQHLDVHSRISNSNNMMNNNDKTKESLSCLLSIGKKYDKSLEKSQSDDNVVKKHQFCLFGQPILTEQQISRNCSEKNSVDDSKDKEKWFLDAFSAGKGSNTAEFSWQLGLDIGHCKVFLESEDVGRTLDLSLLGSYEELYKKLANMFDLEKSEMLNHVFYRDATGAVKQIGEEPFSEFMKTAKRLTILTDSGSKNVRGAWITGTHKGEHGLDASNKTGPLSIFA*