AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020638 MtrunA17_Chr4g0028991 95.657 852 19 2 1 840 1 846 0.0 1677
MsaG020638 MtrunA17_Chr2g0326731 76.553 853 171 9 3 840 1 839 0.0 1349
MsaG020638 MtrunA17_Chr2g0292391 66.942 847 241 10 19 840 4 836 0.0 1147
MsaG020638 MtrunA17_Chr2g0331131 63.938 843 262 11 24 840 10 836 0.0 1079
MsaG020638 MtrunA17_Chr8g0340411 62.928 847 266 13 21 840 7 832 0.0 1070
MsaG020638 MtrunA17_Chr3g0140351 62.874 835 277 10 24 840 16 835 0.0 1067
MsaG020638 MtrunA17_Chr2g0331581 22.885 520 301 16 29 477 143 633 4.32e-19 92.8
MsaG020638 MtrunA17_Chr7g0224451 22.944 462 284 14 7 421 1 437 4.01e-17 85.1
MsaG020638 MtrunA17_Chr7g0224481 24.242 363 206 12 7 324 1 339 2.72e-13 72.0
MsaG020638 MtrunA17_Chr2g0322731 32.479 117 65 3 12 125 87 192 1.05e-12 72.0
MsaG020638 MtrunA17_Chr4g0024351 32.479 117 71 2 9 125 44 152 3.58e-12 70.1
MsaG020638 MtrunA17_Chr5g0410711 34.694 98 56 2 28 125 92 181 2.61e-11 67.4
MsaG020638 MtrunA17_Chr6g0481041 30.058 173 90 5 18 190 22 163 4.13e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020638 AT5G60690.1 78.738 856 150 9 3 840 1 842 0.0 1370
MsaG020638 AT2G34710.1 66.315 852 242 14 19 840 16 852 0.0 1117
MsaG020638 AT1G30490.1 64.468 864 253 13 7 840 2 841 0.0 1107
MsaG020638 AT1G52150.2 64.734 845 250 13 24 840 13 837 0.0 1081
MsaG020638 AT1G52150.1 64.734 845 249 13 24 840 13 836 0.0 1079
MsaG020638 AT4G32880.1 62.632 851 271 17 17 840 3 833 0.0 1049
MsaG020638 AT1G52150.3 64.573 796 234 12 24 791 13 788 0.0 1009
MsaG020638 AT5G32670.1 67.143 70 23 0 292 361 37 106 6.68e-26 106
MsaG020638 AT5G52170.2 24.474 380 231 12 29 371 61 421 6.43e-13 73.2
MsaG020638 AT5G52170.3 24.474 380 231 12 29 371 61 421 6.51e-13 73.2
MsaG020638 AT5G52170.1 24.474 380 231 12 29 371 61 421 6.51e-13 73.2
MsaG020638 AT4G04890.2 35.714 98 55 2 28 125 65 154 1.28e-12 72.0
MsaG020638 AT4G04890.1 35.714 98 55 2 28 125 65 154 1.28e-12 72.0
MsaG020638 AT4G21750.2 35.714 98 55 2 28 125 65 154 4.04e-12 70.5
MsaG020638 AT4G21750.1 35.714 98 55 2 28 125 65 154 4.04e-12 70.5
MsaG020638 AT4G21750.3 35.714 98 55 2 28 125 65 154 4.04e-12 70.5
MsaG020638 AT4G21750.4 35.714 98 55 2 28 125 65 154 4.04e-12 70.5
MsaG020638 AT1G05230.8 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.4 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.9 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.10 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.7 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.11 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.2 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.6 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.5 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.1 35.714 98 55 2 28 125 67 156 7.83e-12 69.7
MsaG020638 AT1G05230.3 35.714 98 55 2 28 125 67 156 8.51e-12 69.3
MsaG020638 AT1G05230.12 35.714 98 55 2 28 125 67 156 1.57e-11 68.6
MsaG020638 AT1G73360.1 35.714 98 55 2 28 125 35 124 2.47e-11 68.2

Find 217 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTAATCAGTTACTATT+TGG 0.070575 4:-55946650 MsaT020638.1:intron
TGGCTCCGGCCCGGGATTTC+TGG 0.116971 4:-55949062 MsaT020638.1:CDS
CAAGAAGATGCTTTGTTTCT+AGG 0.156382 4:-55947620 MsaT020638.1:CDS
TAGCTTTCTCTTTCATAAAT+TGG 0.177453 4:-55946041 MsaT020638.1:CDS
CTCAATGGTTCCTCCATTTC+CGG 0.218751 4:+55949194 None:intergenic
AGAAGAGCTACTTAGATTCT+TGG 0.227127 4:+55948183 None:intergenic
TGAGCAGCAGCAGTTGAATT+AGG 0.229833 4:+55948875 None:intergenic
CCTTTAATCAGTTACTATTT+GGG 0.264850 4:-55946649 MsaT020638.1:intron
AATGGATCTGCCAGAGTTAT+AGG 0.277310 4:-55946755 MsaT020638.1:intron
GAAACATTGTGAAAACTTCT+AGG 0.277838 4:+55949220 None:intergenic
ACCAAGATAAGCGATCTTTA+AGG 0.277977 4:+55949259 None:intergenic
GATGCTCCATTGGTGCCTTC+TGG 0.293806 4:-55947280 MsaT020638.1:CDS
TGAGCTAGTGTAACTGCTTC+TGG 0.294323 4:+55946777 None:intergenic
GGCTTCAATGACGCTGTAAA+TGG 0.300316 4:-55948277 MsaT020638.1:CDS
CTGAGATCAGATTCTCTTGT+TGG 0.314455 4:-55946616 MsaT020638.1:CDS
GTGGCTACCTGCTCAATCTT+TGG 0.322456 4:+55948424 None:intergenic
GAAAGGTCCCTGTCTGGTTC+TGG 0.324143 4:-55948905 MsaT020638.1:CDS
TGATTGCCAGTGAATCTTGT+AGG 0.327004 4:+55947887 None:intergenic
TGCAGATGCTTGAAGTTATT+AGG 0.332303 4:-55947664 MsaT020638.1:intron
TGGCCAGCCTGGTCCGGATT+CGG 0.332357 4:-55949489 MsaT020638.1:intron
GATAGGCACTTCGATATATT+AGG 0.337752 4:-55948521 MsaT020638.1:intron
TAAAGTGGTCGTAGTACTTC+TGG 0.341715 4:+55948663 None:intergenic
TCAACACTGATCGGGTGTTT+TGG 0.344060 4:+55946964 None:intergenic
TCCCTGTCTGGTTCTGGCAC+TGG 0.345502 4:-55948899 MsaT020638.1:CDS
GTTGATTGGGTCCAGATGCC+TGG 0.352645 4:-55949910 MsaT020638.1:CDS
TCTGGGAACATTTCATCAAT+TGG 0.355893 4:+55947304 None:intergenic
CTTGAAGTTATTAGGCTTGA+AGG 0.356810 4:-55947656 MsaT020638.1:CDS
GGGTTTGCTTATCTGCCAGC+TGG 0.357471 4:-55946156 MsaT020638.1:CDS
GGCACCAATGGAGCATCATC+TGG 0.365656 4:+55947286 None:intergenic
ACATTGAAATCAGCCCATTC+TGG 0.374959 4:+55947968 None:intergenic
TCTTAGATACACTACAACTT+TGG 0.376901 4:-55949037 MsaT020638.1:CDS
TGGAGAACCAGGAGAAAGTT+TGG 0.380345 4:+55946797 None:intergenic
ATTGATAAGCATCTAGATTC+AGG 0.380570 4:-55950983 MsaT020638.1:CDS
ACAATGTTTCCTGCCGGAAA+TGG 0.381342 4:-55949207 MsaT020638.1:CDS
AGTATGGAATTTACCTTTGT+AGG 0.381444 4:+55949395 None:intergenic
CGGGCCGGAGCCAGCGTTGT+TGG 0.382892 4:+55949072 None:intergenic
TTCTCTTGGCCAGCCTGGTC+CGG 0.383123 4:-55949495 MsaT020638.1:intron
GGTGAAGTGGTATACGGATT+AGG 0.388453 4:-55948477 MsaT020638.1:CDS
AACCACCTGTTGCATTAACT+TGG 0.391447 4:+55946266 None:intergenic
TCCTTAAAGATCGCTTATCT+TGG 0.392201 4:-55949260 MsaT020638.1:CDS
ATCAAATAATCATGCCTCTT+GGG 0.394989 4:-55947868 MsaT020638.1:CDS
AACCAAACCTTGATCTGTTT+AGG 0.396617 4:+55950833 None:intergenic
CAAGGCTTCAAACTGTGAAT+AGG 0.399527 4:-55950670 MsaT020638.1:CDS
GAAAGTTTGGCACCAACAGC+TGG 0.405904 4:+55946810 None:intergenic
AGACCTTCATCCCAGGCATC+TGG 0.407117 4:+55949899 None:intergenic
CAACAACGCTGGCTCCGGCC+CGG 0.411682 4:-55949071 MsaT020638.1:CDS
AACAACGCTGGCTCCGGCCC+GGG 0.413344 4:-55949070 MsaT020638.1:CDS
AAGAAGATGCTTTGTTTCTA+GGG 0.413799 4:-55947619 MsaT020638.1:CDS
TCGAGTGGTGAAGTGGTATA+CGG 0.413960 4:-55948483 MsaT020638.1:CDS
TAGATTCAGGGAAATATGTT+AGG 0.416438 4:-55950970 MsaT020638.1:CDS
CTACAGGAACTGCTGTTGAT+TGG 0.418349 4:-55949924 MsaT020638.1:CDS
TACAGGAACTGCTGTTGATT+GGG 0.419994 4:-55949923 MsaT020638.1:CDS
GCAAAGATCCCAACCGAATC+CGG 0.421507 4:+55949476 None:intergenic
GAGCAGCAGCAGTTGAATTA+GGG 0.422108 4:+55948876 None:intergenic
GTTGGTGCCAAACTTTCTCC+TGG 0.425700 4:-55946804 MsaT020638.1:CDS
TGTTGTCTTCTCATGGAACT+AGG 0.427476 4:+55950893 None:intergenic
GGGATCTGCATGTCGACAAT+GGG 0.430271 4:-55946135 MsaT020638.1:CDS
CAGTGTCCGGTTTGTATTCA+CGG 0.430832 4:+55947052 None:intergenic
AACCTAAACAGATCAAGGTT+TGG 0.433904 4:-55950835 MsaT020638.1:CDS
AATCAAATAATCATGCCTCT+TGG 0.435473 4:-55947869 MsaT020638.1:CDS
CAATGAACAGTGTTATCTTC+TGG 0.436228 4:+55946066 None:intergenic
CACTGCCGTGAGTTGTCGTT+GGG 0.437038 4:+55947003 None:intergenic
GCAGGAACCTATGCCTATCC+AGG 0.445397 4:-55947917 MsaT020638.1:CDS
GCAAGTTCCCTCACATTGCT+TGG 0.446182 4:-55948154 MsaT020638.1:CDS
GATAAAATATTTGACGAGTC+TGG 0.446806 4:-55946315 MsaT020638.1:CDS
GCAAGATAATGTTGTAATCA+TGG 0.449611 4:-55946907 MsaT020638.1:CDS
AGCAAAGAAAAGAGGCTTCA+AGG 0.453264 4:-55950688 MsaT020638.1:CDS
AATCAATTGTTGTCTTCTCA+TGG 0.455291 4:+55950886 None:intergenic
CTCTGTGCAGAGGGTTGCCA+TGG 0.455799 4:-55946856 MsaT020638.1:CDS
GTTTATACCGGACGGAGAAA+TGG 0.458027 4:+55946833 None:intergenic
GCCATGGCCATTTCTCCGTC+CGG 0.458111 4:-55946840 MsaT020638.1:CDS
AGCAATGTGAGGGAACTTGC+TGG 0.458432 4:+55948157 None:intergenic
GGTTCAATGTTGGAAAGAAT+AGG 0.460052 4:+55950854 None:intergenic
TGCTCCAACAACGCTGGCTC+CGG 0.460616 4:-55949076 MsaT020638.1:CDS
CAGCCTGGTCCGGATTCGGT+TGG 0.460852 4:-55949485 MsaT020638.1:CDS
GTTTGTGAAAGGTCCCTGTC+TGG 0.462237 4:-55948911 MsaT020638.1:CDS
AAGTCTCCTCCGCAATTGAT+AGG 0.463644 4:+55949968 None:intergenic
GGTCCAGATGCCTGGGATGA+AGG 0.464996 4:-55949902 MsaT020638.1:intron
AGCCTGGTCCGGATTCGGTT+GGG 0.465213 4:-55949484 MsaT020638.1:CDS
CATTGAACCTAAACAGATCA+AGG 0.465302 4:-55950840 MsaT020638.1:CDS
TTTGGTATTGCAGTCGCAGC+CGG 0.467861 4:-55946412 MsaT020638.1:intron
TGTTAGCACTTTCCATGCAA+TGG 0.472949 4:+55946089 None:intergenic
CTTCTCATGGAACTAGGCTT+AGG 0.473637 4:+55950899 None:intergenic
TGTAGACTTGCCTATAACTC+TGG 0.480673 4:+55946745 None:intergenic
TGTTGATGGAGGAAAATGAT+AGG 0.483631 4:-55950625 MsaT020638.1:CDS
TACTGAACAAGTTGAAGCAT+TGG 0.483862 4:-55950942 MsaT020638.1:CDS
ATCTAAGAGTCCAGAAATCC+CGG 0.484814 4:+55949052 None:intergenic
TCTTGCATAGTGCTGTCAAA+AGG 0.484830 4:+55946924 None:intergenic
TAAGGGAACACCGCCAGAAT+GGG 0.485358 4:-55947981 MsaT020638.1:intron
GCATCAGTATTTGCTGCTGA+TGG 0.488575 4:+55950150 None:intergenic
GTATTACTTCAAGATTTAAG+TGG 0.492819 4:+55948771 None:intergenic
AGAGATGCTACTAACCCTGC+TGG 0.494241 4:-55950087 MsaT020638.1:CDS
ACCTTTGGTGATTCATAAAG+TGG 0.494265 4:+55948648 None:intergenic
GCACTGCCGTGAGTTGTCGT+TGG 0.495965 4:+55947002 None:intergenic
TCACAGCTTGTGTGTGAGAA+TGG 0.498450 4:-55950587 MsaT020638.1:CDS
TGCAATGAACAAGTTGTTGA+TGG 0.500516 4:-55950639 MsaT020638.1:CDS
CTGAATGTTCGCAAAACAGC+AGG 0.501563 4:+55948447 None:intergenic
TTTGTATTTGCAAATCAAGC+TGG 0.503494 4:-55946387 MsaT020638.1:CDS
ATCCCAACCGAATCCGGACC+AGG 0.503586 4:+55949482 None:intergenic
TCAAGCTGGCCTTGACATGT+TGG 0.506180 4:-55946373 MsaT020638.1:CDS
CTTGCATAGTGCTGTCAAAA+GGG 0.508484 4:+55946925 None:intergenic
GGCCAGTGCCAGAACCAGAC+AGG 0.510237 4:+55948897 None:intergenic
TATCTTGGTTTCATGAGTGT+CGG 0.513832 4:-55949245 MsaT020638.1:CDS
GGTTTGCTTATCTGCCAGCT+GGG 0.514174 4:-55946155 MsaT020638.1:CDS
ATATATTAGGCAAATAGCTC+AGG 0.515140 4:-55948508 MsaT020638.1:CDS
ACAAGTTGAAGCATTGGAAA+GGG 0.515957 4:-55950936 MsaT020638.1:CDS
AGGCAATAGTCAACACTGAT+CGG 0.516074 4:+55946955 None:intergenic
GAGTTCCTTTCAAAGGCTAC+AGG 0.517677 4:-55949940 MsaT020638.1:CDS
GGCAATAGTCAACACTGATC+GGG 0.517701 4:+55946956 None:intergenic
TTGATTGGGTCCAGATGCCT+GGG 0.519139 4:-55949909 MsaT020638.1:CDS
AAGCAGTTACACTAGCTCAA+TGG 0.520279 4:-55946773 MsaT020638.1:CDS
TTCTTCTTGCAGATATGTAG+TGG 0.520407 4:-55947373 MsaT020638.1:CDS
ACCAACAGCTGGGTTTATAC+CGG 0.520732 4:+55946821 None:intergenic
GTCGACATGCAGATCCCAGC+TGG 0.520952 4:+55946141 None:intergenic
CGGACACTGGATTTGACGTC+AGG 0.521504 4:-55947038 MsaT020638.1:CDS
GATCTGTTTAGGTTCAATGT+TGG 0.526382 4:+55950844 None:intergenic
ATAGAAAGTAGTAGTAGTGG+TGG 0.530086 4:-55951010 MsaT020638.1:CDS
TGGGATCTGCATGTCGACAA+TGG 0.533448 4:-55946136 MsaT020638.1:CDS
CATGGCTATGGCTATGGCTG+TGG 0.534941 4:-55951041 MsaT020638.1:CDS
CTAAGGGAACACCGCCAGAA+TGG 0.536751 4:-55947982 MsaT020638.1:intron
CTGTGTGTAAATAAGCTCAA+TGG 0.538636 4:+55949179 None:intergenic
AAGAAACATGGCTATGGCTA+TGG 0.538767 4:-55951047 None:intergenic
ATGAGGCCTACAAGATTCAC+TGG 0.543583 4:-55947893 MsaT020638.1:CDS
TTTGACAGAGTTCCTTTCAA+AGG 0.544138 4:-55949947 MsaT020638.1:CDS
TTGATAAGCATCTAGATTCA+GGG 0.546019 4:-55950982 MsaT020638.1:CDS
CGGAAACCAGAAGGCACCAA+TGG 0.546575 4:+55947274 None:intergenic
GTAACTGCTTCTGGAGAACC+AGG 0.547189 4:+55946786 None:intergenic
AATCTTGTAGGCCTCATCCC+TGG 0.549464 4:+55947899 None:intergenic
CAGTTCCTGTAGCCTTTGAA+AGG 0.551448 4:+55949935 None:intergenic
TGTGATTTCCTCTGTGCAGA+GGG 0.551770 4:-55946865 MsaT020638.1:CDS
AACAAGTTGAAGCATTGGAA+AGG 0.552407 4:-55950937 MsaT020638.1:CDS
AGGATGCCGTGAATACAAAC+CGG 0.553865 4:-55947058 MsaT020638.1:CDS
ATGGTTCCTCCATTTCCGGC+AGG 0.554780 4:+55949198 None:intergenic
CTTTAATCAGTTACTATTTG+GGG 0.555387 4:-55946648 MsaT020638.1:intron
CTGACGTCAAATCCAGTGTC+CGG 0.556266 4:+55947039 None:intergenic
AACAGCATAAAATCGCGTCC+TGG 0.557589 4:+55946563 None:intergenic
TTATGAATCACCAAAGGTGG+TGG 0.558033 4:-55948643 MsaT020638.1:CDS
GAAACAACTGTGGCATCACC+AGG 0.558649 4:-55946581 MsaT020638.1:CDS
GGACTGATGAGAACGCTGTT+GGG 0.559056 4:-55947351 MsaT020638.1:CDS
TCTTCTTGCAGATATGTAGT+GGG 0.559916 4:-55947372 MsaT020638.1:CDS
CGTACGAGCAAGCCATTGCA+TGG 0.562208 4:-55946101 MsaT020638.1:CDS
GCGATTTCACAAAGTTGTAG+TGG 0.563887 4:-55949455 MsaT020638.1:CDS
TGTAGGCCTCATCCCTGGAT+AGG 0.565752 4:+55947904 None:intergenic
GTTCCCAGATGATGCTCCAT+TGG 0.566789 4:-55947290 MsaT020638.1:CDS
TCCTGCTTTCAGTGATGCAG+CGG 0.568391 4:+55947934 None:intergenic
TAATAGTGAACTTACTGCAA+TGG 0.570234 4:+55948608 None:intergenic
ATTTGACGAGTCTGGTCGCA+AGG 0.571396 4:-55946307 MsaT020638.1:CDS
TCCGCTGCATCACTGAAAGC+AGG 0.573762 4:-55947935 MsaT020638.1:CDS
GTGTGATTTCCTCTGTGCAG+AGG 0.574274 4:-55946866 MsaT020638.1:CDS
AAAGTTTGGCACCAACAGCT+GGG 0.574992 4:+55946811 None:intergenic
TTCACAAAGTTGTAGTGGAG+TGG 0.575629 4:-55949450 MsaT020638.1:CDS
CCTCATCCCTGGATAGGCAT+AGG 0.580110 4:+55947910 None:intergenic
CATTCAGCCAAAGATTGAGC+AGG 0.580203 4:-55948431 MsaT020638.1:intron
ATGTTTCCTGCCGGAAATGG+AGG 0.580359 4:-55949204 MsaT020638.1:CDS
TCCGGTATAAACCCAGCTGT+TGG 0.580698 4:-55946822 MsaT020638.1:CDS
GCACCAATGGAGCATCATCT+GGG 0.585521 4:+55947287 None:intergenic
TAGAGAGAAGCAAAGAAAAG+AGG 0.587959 4:-55950696 MsaT020638.1:CDS
AGTTCCCTCACATTGCTTGG+AGG 0.589621 4:-55948151 MsaT020638.1:CDS
GTAAATGGATTCAGTGATGA+TGG 0.590567 4:-55948262 MsaT020638.1:CDS
GTGAACCCAACGACAACTCA+CGG 0.590708 4:-55947008 MsaT020638.1:CDS
AGCTTTACAAGACATTACAT+TGG 0.590794 4:-55946337 MsaT020638.1:CDS
CGTGAATACAAACCGGACAC+TGG 0.591523 4:-55947051 MsaT020638.1:CDS
GAGTTGTTTCCAACATGTCA+AGG 0.591719 4:+55946364 None:intergenic
GGGACTGATGAGAACGCTGT+TGG 0.594775 4:-55947352 MsaT020638.1:CDS
GAACACAATTGAACATGAAG+AGG 0.595332 4:-55947846 MsaT020638.1:intron
AATCAGTTACTATTTGGGGA+CGG 0.595776 4:-55946644 MsaT020638.1:intron
TGTTAGTTTAGAACCTACAA+AGG 0.596128 4:-55949408 MsaT020638.1:intron
AGGTTTGGTTCCAAAATCGA+AGG 0.597184 4:-55950820 MsaT020638.1:intron
ATTCGACCATGTGATGGTGG+CGG 0.598224 4:-55948815 MsaT020638.1:CDS
TGTTCAATTGTGTTCCCAAG+AGG 0.599573 4:+55947854 None:intergenic
AAATAAGAGACCTTCATCCC+AGG 0.600192 4:+55949892 None:intergenic
GTCGAATCAAGTAGCCACTC+AGG 0.601710 4:+55948831 None:intergenic
TTGCAGACTCCTATCAATTG+CGG 0.602778 4:-55949977 MsaT020638.1:intron
CATCTTCTTGAGCAAGAGAG+TGG 0.603372 4:+55947633 None:intergenic
CATATAGAAAGTAGTAGTAG+TGG 0.603914 4:-55951013 MsaT020638.1:CDS
GAGATGCTACTAACCCTGCT+GGG 0.605907 4:-55950086 MsaT020638.1:intron
GACATATGCTCCAACAACGC+TGG 0.606931 4:-55949082 MsaT020638.1:CDS
TGATTTGCAAATACAAACAC+CGG 0.610151 4:+55946393 None:intergenic
AGTACTTCTGGCACACTCCA+CGG 0.612200 4:+55948675 None:intergenic
GGGATGATGCGGAAACCAGA+AGG 0.615520 4:+55947265 None:intergenic
TCAGGAATCGAGTGGTGAAG+TGG 0.617819 4:-55948490 MsaT020638.1:CDS
GCCAGTGCCAGAACCAGACA+GGG 0.617845 4:+55948898 None:intergenic
TTGAATCTAATGGGATGATG+CGG 0.619863 4:+55947254 None:intergenic
GCATCTCTCTTAGAATTCTG+AGG 0.619916 4:+55950102 None:intergenic
CATTGAGCTTATTTACACAC+AGG 0.620551 4:-55949178 MsaT020638.1:intron
GAATTCCTCCAAGCAATGTG+AGG 0.621910 4:+55948146 None:intergenic
AGAGTCCAGAAATCCCGGGC+CGG 0.624215 4:+55949057 None:intergenic
CAGGAACCTATGCCTATCCA+GGG 0.626828 4:-55947916 MsaT020638.1:CDS
CCTATGCCTATCCAGGGATG+AGG 0.627418 4:-55947910 MsaT020638.1:CDS
ATGGATTCAGTGATGATGGT+TGG 0.628950 4:-55948258 MsaT020638.1:CDS
TACACTACAACTTTGGACAA+TGG 0.630488 4:-55949030 MsaT020638.1:CDS
CAGACTCCTATCAATTGCGG+AGG 0.631681 4:-55949974 MsaT020638.1:CDS
TACTTGATTCGACCATGTGA+TGG 0.633229 4:-55948821 MsaT020638.1:CDS
TGTGGGAAGAAACATGGCTA+TGG 0.633966 4:-55951053 None:intergenic
AATTCCTCCAAGCAATGTGA+GGG 0.636010 4:+55948147 None:intergenic
GGAGTGGCAGCTCGAGCTTG+CGG 0.638597 4:-55949434 MsaT020638.1:CDS
TTTGGACAATGGCAGTCTTG+TGG 0.639500 4:-55949019 MsaT020638.1:intron
ATTGAACCGCCACCATCACA+TGG 0.640356 4:+55948809 None:intergenic
TCTAAGAGTCCAGAAATCCC+GGG 0.642124 4:+55949053 None:intergenic
GATTTAAGTGGTCTACAATG+TGG 0.642422 4:+55948783 None:intergenic
ACCGGACGGAGAAATGGCCA+TGG 0.644894 4:+55946839 None:intergenic
TTGATTCGACCATGTGATGG+TGG 0.651436 4:-55948818 MsaT020638.1:CDS
TGGTCTATACTGAACTGTGA+CGG 0.652371 4:-55948238 MsaT020638.1:CDS
ACTTTATGAATCACCAAAGG+TGG 0.655976 4:-55948646 MsaT020638.1:CDS
TCTGCATGTCGACAATGGGG+CGG 0.660182 4:-55946131 MsaT020638.1:CDS
GCTGTCAAAAGGGAACTGAA+AGG 0.661239 4:+55946935 None:intergenic
TTGAAATCAGCCCATTCTGG+CGG 0.662790 4:+55947971 None:intergenic
ACAGCTGGGTTTATACCGGA+CGG 0.670270 4:+55946825 None:intergenic
GGATCTGCATGTCGACAATG+GGG 0.672158 4:-55946134 MsaT020638.1:CDS
ACTGAACTGTGACGGCGCTG+AGG 0.673744 4:-55948230 MsaT020638.1:CDS
ACCACTTTATGAATCACCAA+AGG 0.678609 4:-55948649 MsaT020638.1:CDS
CAAATAGCTCAGGAATCGAG+TGG 0.680372 4:-55948498 MsaT020638.1:CDS
TGTGTGAGAATGGATTTATG+AGG 0.695971 4:-55950577 MsaT020638.1:CDS
AATGAACAAGTTGTTGATGG+AGG 0.713928 4:-55950636 MsaT020638.1:CDS
CATGGCAACCCTCTGCACAG+AGG 0.723163 4:+55946857 None:intergenic
AGAGCTGAAATGCTCCTGAG+TGG 0.728883 4:-55948845 MsaT020638.1:CDS
ACTTGATGCTGAAACAACTG+TGG 0.757022 4:-55946591 MsaT020638.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 55946029 55951062 55946029 ID=MsaG020638
Chr4 mRNA 55946029 55951062 55946029 ID=MsaT020638.1;Parent=MsaG020638
Chr4 exon 55946029 55946178 55946029 ID=MsaT020638.1.exon18;Parent=MsaT020638.1
Chr4 CDS 55946029 55946178 55946029 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55946272 55946421 55946272 ID=MsaT020638.1.exon17;Parent=MsaT020638.1
Chr4 CDS 55946272 55946421 55946272 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55946555 55946660 55946555 ID=MsaT020638.1.exon16;Parent=MsaT020638.1
Chr4 CDS 55946555 55946660 55946555 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55946756 55947090 55946756 ID=MsaT020638.1.exon15;Parent=MsaT020638.1
Chr4 CDS 55946756 55947090 55946756 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55947249 55947409 55947249 ID=MsaT020638.1.exon14;Parent=MsaT020638.1
Chr4 CDS 55947249 55947409 55947249 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55947609 55947681 55947609 ID=MsaT020638.1.exon13;Parent=MsaT020638.1
Chr4 CDS 55947609 55947681 55947609 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55947847 55947999 55947847 ID=MsaT020638.1.exon12;Parent=MsaT020638.1
Chr4 CDS 55947847 55947999 55947847 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55948117 55948300 55948117 ID=MsaT020638.1.exon11;Parent=MsaT020638.1
Chr4 CDS 55948117 55948300 55948117 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55948432 55948538 55948432 ID=MsaT020638.1.exon10;Parent=MsaT020638.1
Chr4 CDS 55948432 55948538 55948432 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55948623 55948697 55948623 ID=MsaT020638.1.exon9;Parent=MsaT020638.1
Chr4 CDS 55948623 55948697 55948623 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55948778 55948933 55948778 ID=MsaT020638.1.exon8;Parent=MsaT020638.1
Chr4 CDS 55948778 55948933 55948778 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55949020 55949103 55949020 ID=MsaT020638.1.exon7;Parent=MsaT020638.1
Chr4 CDS 55949020 55949103 55949020 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55949179 55949292 55949179 ID=MsaT020638.1.exon6;Parent=MsaT020638.1
Chr4 CDS 55949179 55949292 55949179 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55949409 55949504 55949409 ID=MsaT020638.1.exon5;Parent=MsaT020638.1
Chr4 CDS 55949409 55949504 55949409 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55949903 55949993 55949903 ID=MsaT020638.1.exon4;Parent=MsaT020638.1
Chr4 CDS 55949903 55949993 55949903 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55950087 55950172 55950087 ID=MsaT020638.1.exon3;Parent=MsaT020638.1
Chr4 CDS 55950087 55950172 55950087 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55950562 55950721 55950562 ID=MsaT020638.1.exon2;Parent=MsaT020638.1
Chr4 CDS 55950562 55950721 55950562 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Chr4 exon 55950821 55951062 55950821 ID=MsaT020638.1.exon1;Parent=MsaT020638.1
Chr4 CDS 55950821 55951062 55950821 ID=cds.MsaT020638.1;Parent=MsaT020638.1
Gene Sequence

>MsaT020638.1

ATGGCTATGGCTATGGCTGTGGCTCATCATATAGAAAGTAGTAGTAGTGGTGGAAGCATTGATAAGCATCTAGATTCAGGGAAATATGTTAGGTACACTACTGAACAAGTTGAAGCATTGGAAAGGGTTTATGCTGAGTGTCCTAAGCCTAGTTCCATGAGAAGACAACAATTGATTCGTGAGTGTCCTATTCTTTCCAACATTGAACCTAAACAGATCAAGGTTTGGTTCCAAAATCGAAGGTGTAGAGAGAAGCAAAGAAAAGAGGCTTCAAGGCTTCAAACTGTGAATAGGAAATTGTCTGCAATGAACAAGTTGTTGATGGAGGAAAATGATAGGTTGCAGAAACAAGTGTCACAGCTTGTGTGTGAGAATGGATTTATGAGGCAACAATTGCATACTCCATCAGCAGCAAATACTGATGCAAGTTGTGACTCAGTAGTTACTACTCCTCAGAATTCTAAGAGAGATGCTACTAACCCTGCTGGACTCCTATCAATTGCGGAGGAGACTTTGACAGAGTTCCTTTCAAAGGCTACAGGAACTGCTGTTGATTGGGTCCAGATGCCTGGGATGAAGCCTGGTCCGGATTCGGTTGGGATCTTTGCGATTTCACAAAGTTGTAGTGGAGTGGCAGCTCGAGCTTGCGGTCTTGTTAGTTTAGAACCTACAAAGATTGCAGAGATCCTTAAAGATCGCTTATCTTGGTTTCATGAGTGTCGGAACCTAGAAGTTTTCACAATGTTTCCTGCCGGAAATGGAGGAACCATTGAGCTTATTTACACACAGACATATGCTCCAACAACGCTGGCTCCGGCCCGGGATTTCTGGACTCTTAGATACACTACAACTTTGGACAATGGCAGTCTTGTGGTTTGTGAAAGGTCCCTGTCTGGTTCTGGCACTGGCCCTAATTCAACTGCTGCTGCTCAGTTTGTAAGAGCTGAAATGCTCCTGAGTGGCTACTTGATTCGACCATGTGATGGTGGCGGTTCAATCATCCACATTGTAGACCACTTAAATCTTGAACCGTGGAGTGTGCCAGAAGTACTACGACCACTTTATGAATCACCAAAGGTGGTGGCTCAAAAAATGACCATTGCAGCACTTCGATATATTAGGCAAATAGCTCAGGAATCGAGTGGTGAAGTGGTATACGGATTAGGTAGACAGCCTGCTGTTTTGCGAACATTCAGCCAAAGATTGAGCAGAGGCTTCAATGACGCTGTAAATGGATTCAGTGATGATGGTTGGTCTATACTGAACTGTGACGGCGCTGAGGATGTAATTATTGCTGTTAATTCAACCAAGAATCTAAGTAGCTCTTCTAATCCAGCAAGTTCCCTCACATTGCTTGGAGGAATTCTCTGTGCAAAAGCTTCTATGTTGCTACAAGGAACACCGCCAGAATGGGCTGATTTCAATGTTGATGCATACTCCGCTGCATCACTGAAAGCAGGAACCTATGCCTATCCAGGGATGAGGCCTACAAGATTCACTGGCAATCAAATAATCATGCCTCTTGGGAACACAATTGAACATGAAGAGATGCTTGAAGTTATTAGGCTTGAAGGCCACTCTCTTGCTCAAGAAGATGCTTTGTTTCTAGGGATATTCACCTGTGTTTTATGTGTATTCTTCTTGCAGATATGTAGTGGGACTGATGAGAACGCTGTTGGGACTTGTTCTGAGCTCATATTTGCTCCAATTGATGAAATGTTCCCAGATGATGCTCCATTGGTGCCTTCTGGTTTCCGCATCATCCCATTAGATTCAAAACCAGGGGATAAGAAGGATGCCGTGAATACAAACCGGACACTGGATTTGACGTCAGGTCTTGAAGTGAACCCAACGACAACTCACGGCAGTGCAGATTCATCATCAAGCCAAAACACCCGATCAGTGTTGACTATTGCCTTTCAGTTCCCTTTTGACAGCACTATGCAAGATAATGTTGTAATCATGGCACGTCAATATGTTCGTAGTGTGATTTCCTCTGTGCAGAGGGTTGCCATGGCCATTTCTCCGTCCGGTATAAACCCAGCTGTTGGTGCCAAACTTTCTCCTGGTTCTCCAGAAGCAGTTACACTAGCTCAATGGATCTGCCAGAGTTATAGTTACTATTTGGGGACGGACTTACTGAGATCAGATTCTCTTGTTGGTGACTTGATGCTGAAACAACTGTGGCATCACCAGGACGCGATTTTATGCTGTTCATTGAAGTCGCAGCCGGTGTTTGTATTTGCAAATCAAGCTGGCCTTGACATGTTGGAAACAACTCTAGTAGCTTTACAAGACATTACATTGGATAAAATATTTGACGAGTCTGGTCGCAAGGCGTTGTGTGCTGATTTTGCCAAGTTAATGCAACAGGGGTTTGCTTATCTGCCAGCTGGGATCTGCATGTCGACAATGGGGCGGCATGTTTCGTACGAGCAAGCCATTGCATGGAAAGTGCTAACACCAGAAGATAACACTGTTCATTGCTTAGCTTTCTCTTTCATAAATTGGTCATTTGTATGA
Protein sequence

>MsaT020638.1

MAMAMAVAHHIESSSSGGSIDKHLDSGKYVRYTTEQVEALERVYAECPKPSSMRRQQLIRECPILSNIEPKQIKVWFQNRRCREKQRKEASRLQTVNRKLSAMNKLLMEENDRLQKQVSQLVCENGFMRQQLHTPSAANTDASCDSVVTTPQNSKRDATNPAGLLSIAEETLTEFLSKATGTAVDWVQMPGMKPGPDSVGIFAISQSCSGVAARACGLVSLEPTKIAEILKDRLSWFHECRNLEVFTMFPAGNGGTIELIYTQTYAPTTLAPARDFWTLRYTTTLDNGSLVVCERSLSGSGTGPNSTAAAQFVRAEMLLSGYLIRPCDGGGSIIHIVDHLNLEPWSVPEVLRPLYESPKVVAQKMTIAALRYIRQIAQESSGEVVYGLGRQPAVLRTFSQRLSRGFNDAVNGFSDDGWSILNCDGAEDVIIAVNSTKNLSSSSNPASSLTLLGGILCAKASMLLQGTPPEWADFNVDAYSAASLKAGTYAYPGMRPTRFTGNQIIMPLGNTIEHEEMLEVIRLEGHSLAQEDALFLGIFTCVLCVFFLQICSGTDENAVGTCSELIFAPIDEMFPDDAPLVPSGFRIIPLDSKPGDKKDAVNTNRTLDLTSGLEVNPTTTHGSADSSSSQNTRSVLTIAFQFPFDSTMQDNVVIMARQYVRSVISSVQRVAMAISPSGINPAVGAKLSPGSPEAVTLAQWICQSYSYYLGTDLLRSDSLVGDLMLKQLWHHQDAILCCSLKSQPVFVFANQAGLDMLETTLVALQDITLDKIFDESGRKALCADFAKLMQQGFAYLPAGICMSTMGRHVSYEQAIAWKVLTPEDNTVHCLAFSFINWSFV*