AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020673

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020673 MtrunA17_Chr4g0029671 96.591 792 23 1 1 788 1 792 0.0 1593
MsaG020673 MtrunA17_Chr2g0326281 71.411 829 190 12 1 788 1 823 0.0 1136
MsaG020673 MtrunA17_Chr4g0046371 50.415 482 219 8 49 523 33 501 1.33e-145 444
MsaG020673 MtrunA17_Chr2g0282961 48.413 504 226 9 46 528 45 535 1.71e-144 438
MsaG020673 MtrunA17_Chr8g0385791 38.898 617 347 10 38 649 43 634 5.50e-135 420
MsaG020673 MtrunA17_Chr8g0385791 49.550 111 54 1 654 762 705 815 8.47e-30 126
MsaG020673 MtrunA17_Chr2g0276781 48.831 385 181 4 52 435 21 390 1.53e-123 385
MsaG020673 MtrunA17_Chr2g0276781 50.758 132 62 2 649 777 537 668 1.76e-31 131
MsaG020673 MtrunA17_Chr2g0285461 44.842 475 225 11 54 515 23 473 1.32e-111 361
MsaG020673 MtrunA17_Chr2g0285461 43.617 94 51 2 655 747 771 863 1.09e-18 91.3
MsaG020673 MtrunA17_Chr5g0433301 50.943 371 156 6 54 415 24 377 6.18e-111 357
MsaG020673 MtrunA17_Chr5g0433301 44.186 86 46 2 663 747 719 803 1.73e-17 87.4
MsaG020673 MtrunA17_Chr3g0109391 51.213 371 155 6 54 415 26 379 4.29e-110 355
MsaG020673 MtrunA17_Chr3g0109391 46.512 86 44 2 663 747 727 811 9.46e-18 88.2
MsaG020673 MtrunA17_Chr8g0346711 45.742 411 193 11 52 448 27 421 9.56e-110 350
MsaG020673 MtrunA17_Chr8g0346711 56.044 91 39 1 657 747 556 645 2.70e-25 112
MsaG020673 MtrunA17_Chr8g0376771 50.543 368 161 8 54 415 23 375 6.43e-108 351
MsaG020673 MtrunA17_Chr8g0376771 41.489 94 53 2 655 747 777 869 9.31e-18 88.2
MsaG020673 MtrunA17_Chr4g0009751 47.011 368 175 7 54 416 19 371 4.27e-106 338
MsaG020673 MtrunA17_Chr4g0009751 46.429 84 44 1 643 726 539 621 4.03e-16 82.4
MsaG020673 MtrunA17_Chr1g0156321 51.734 346 152 6 54 396 43 376 1.73e-105 343
MsaG020673 MtrunA17_Chr1g0156321 45.055 91 48 2 659 748 754 843 2.33e-18 90.1
MsaG020673 MtrunA17_Chr7g0239061 45.799 369 181 8 52 416 12 365 3.64e-103 332
MsaG020673 MtrunA17_Chr7g0239061 48.624 109 53 2 642 750 555 660 1.25e-25 112
MsaG020673 MtrunA17_Chr4g0070181 50.720 347 153 5 54 396 23 355 3.07e-98 329
MsaG020673 MtrunA17_Chr4g0070181 47.423 97 49 2 654 749 989 1084 1.14e-21 101
MsaG020673 MtrunA17_Chr2g0301251 49.419 344 162 3 54 396 23 355 1.00e-97 328
MsaG020673 MtrunA17_Chr2g0301251 47.423 97 49 2 654 749 981 1076 4.52e-21 99.4
MsaG020673 MtrunA17_Chr8g0389081 49.422 346 161 7 54 396 32 366 6.66e-93 314
MsaG020673 MtrunA17_Chr8g0389081 47.826 92 46 2 659 749 985 1075 8.99e-22 101
MsaG020673 MtrunA17_Chr2g0326761 41.514 383 172 7 54 399 11 378 1.32e-81 275
MsaG020673 MtrunA17_Chr1g0181211 37.530 413 204 8 54 426 27 425 4.93e-76 260
MsaG020673 MtrunA17_Chr1g0199681 39.901 406 180 11 54 416 94 478 8.61e-76 259
MsaG020673 MtrunA17_Chr4g0028971 38.443 411 186 6 54 416 12 403 7.99e-73 251
MsaG020673 MtrunA17_Chr7g0264171 34.468 470 216 13 54 456 31 475 4.52e-65 228
MsaG020673 MtrunA17_Chr8g0356211 45.455 231 117 4 174 401 2 226 3.07e-61 209
MsaG020673 MtrunA17_Chr3g0115271 35.606 396 207 8 54 416 19 399 1.75e-60 215
MsaG020673 MtrunA17_Chr5g0424931 35.550 391 201 11 54 416 19 386 5.45e-58 206
MsaG020673 MtrunA17_Chr7g0264161 38.580 324 166 8 63 376 1 301 9.80e-58 199
MsaG020673 MtrunA17_Chr5g0417281 34.959 369 204 11 54 414 11 351 8.31e-52 189
MsaG020673 MtrunA17_Chr5g0436761 34.795 365 210 9 54 413 12 353 6.42e-50 182
MsaG020673 MtrunA17_Chr1g0178011 31.720 372 215 9 54 412 12 357 1.10e-46 173
MsaG020673 MtrunA17_Chr5g0417211 34.699 366 192 15 54 409 12 340 2.86e-44 165
MsaG020673 MtrunA17_Chr2g0289221 34.853 307 160 8 54 348 20 298 6.24e-43 159
MsaG020673 MtrunA17_Chr2g0277461 31.148 366 227 8 54 417 13 355 2.05e-39 152
MsaG020673 MtrunA17_Chr5g0424741 28.866 388 240 12 54 417 12 387 6.22e-37 146
MsaG020673 MtrunA17_Chr2g0277531 31.333 300 173 12 54 345 20 294 3.61e-32 127
MsaG020673 MtrunA17_Chr5g0425661 34.928 209 124 3 54 262 37 233 7.46e-32 124
MsaG020673 MtrunA17_Chr5g0424801 28.610 367 234 11 54 414 12 356 8.57e-30 122
MsaG020673 MtrunA17_Chr5g0425261 28.767 365 232 11 54 412 12 354 2.79e-28 117
MsaG020673 MtrunA17_Chr1g0178071 29.630 189 127 3 138 320 48 236 1.10e-11 67.0
MsaG020673 MtrunA17_Chr1g0156301 36.667 90 41 2 659 748 177 250 3.18e-11 64.7
MsaG020673 MtrunA17_Chr7g0276471 36.111 108 68 1 164 271 116 222 7.22e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020673 AT5G60450.2 61.060 755 255 12 48 788 35 764 0.0 850
MsaG020673 AT5G60450.1 61.119 751 253 12 52 788 63 788 0.0 848
MsaG020673 AT2G33860.1 50.311 483 191 9 53 530 53 491 1.94e-146 443
MsaG020673 AT5G62000.2 49.272 412 180 5 52 452 59 452 3.82e-132 414
MsaG020673 AT5G62000.2 53.774 106 49 0 648 753 722 827 8.58e-30 127
MsaG020673 AT5G62000.5 49.272 412 180 5 52 452 59 452 3.82e-132 414
MsaG020673 AT5G62000.5 53.774 106 49 0 648 753 722 827 8.58e-30 127
MsaG020673 AT5G62000.3 49.272 412 180 5 52 452 59 452 3.82e-132 414
MsaG020673 AT5G62000.3 53.774 106 49 0 648 753 722 827 8.58e-30 127
MsaG020673 AT5G62000.1 49.272 412 180 5 52 452 59 452 3.82e-132 414
MsaG020673 AT5G62000.1 53.774 106 49 0 648 753 722 827 8.58e-30 127
MsaG020673 AT5G62000.4 49.272 412 180 5 52 452 59 452 8.17e-132 413
MsaG020673 AT5G62000.4 50.435 115 57 0 648 762 722 836 8.49e-30 127
MsaG020673 AT1G59750.4 48.387 372 165 5 54 416 22 375 1.31e-116 367
MsaG020673 AT1G59750.4 54.545 110 44 1 645 748 517 626 7.50e-30 126
MsaG020673 AT1G59750.2 48.387 372 165 5 54 416 22 375 1.70e-116 367
MsaG020673 AT1G59750.2 54.545 110 44 1 645 748 519 628 7.99e-30 126
MsaG020673 AT1G59750.1 48.387 372 165 5 54 416 22 375 2.25e-116 367
MsaG020673 AT1G59750.1 54.545 110 44 1 645 748 522 631 7.96e-30 126
MsaG020673 AT1G59750.3 48.387 372 165 5 54 416 22 375 2.25e-116 367
MsaG020673 AT1G59750.3 54.545 110 44 1 645 748 522 631 7.96e-30 126
MsaG020673 AT5G37020.2 51.366 366 162 6 54 415 22 375 1.66e-111 357
MsaG020673 AT5G37020.2 50.725 69 32 2 659 726 705 772 6.60e-15 79.3
MsaG020673 AT3G61830.1 49.441 358 164 6 41 396 12 354 2.35e-111 352
MsaG020673 AT3G61830.1 52.000 100 46 2 649 748 480 577 4.45e-24 108
MsaG020673 AT5G37020.1 51.366 366 162 6 54 415 22 375 7.74e-111 357
MsaG020673 AT5G37020.1 46.667 90 46 2 659 747 705 793 1.58e-18 91.3
MsaG020673 AT2G33860.2 51.213 371 139 7 165 530 1 334 3.38e-110 343
MsaG020673 AT1G30330.3 51.491 369 157 8 54 415 25 378 1.29e-108 354
MsaG020673 AT1G30330.3 44.118 102 53 3 649 747 784 884 3.54e-19 93.6
MsaG020673 AT1G30330.2 51.491 369 157 8 54 415 25 378 1.29e-108 354
MsaG020673 AT1G30330.2 44.118 102 53 3 649 747 784 884 3.54e-19 93.6
MsaG020673 AT1G30330.1 51.491 369 157 8 54 415 23 376 1.45e-108 353
MsaG020673 AT1G30330.1 44.118 102 53 3 649 747 782 882 3.56e-19 93.2
MsaG020673 AT2G46530.1 46.757 370 176 7 52 416 19 372 2.98e-108 343
MsaG020673 AT2G46530.1 40.816 147 53 2 634 747 432 577 3.11e-26 114
MsaG020673 AT2G46530.3 46.757 370 176 7 52 416 40 393 8.92e-108 343
MsaG020673 AT2G46530.3 40.816 147 53 2 634 747 453 598 3.74e-26 114
MsaG020673 AT4G23980.1 47.028 387 177 10 52 430 10 376 8.65e-104 333
MsaG020673 AT4G23980.1 53.211 109 49 2 643 750 506 613 5.74e-28 120
MsaG020673 AT4G23980.2 49.003 351 155 8 52 396 10 342 2.21e-103 332
MsaG020673 AT4G23980.2 53.211 109 49 2 643 750 504 611 5.84e-28 120
MsaG020673 AT1G19850.1 49.304 359 165 8 41 396 44 388 1.22e-101 334
MsaG020673 AT1G19850.1 43.564 101 55 2 649 748 783 882 6.74e-18 89.4
MsaG020673 AT1G19850.2 49.304 359 165 8 41 396 33 377 1.42e-101 334
MsaG020673 AT1G19850.2 43.564 101 55 2 649 748 772 871 6.80e-18 89.4
MsaG020673 AT1G19220.1 50.435 345 157 4 54 396 23 355 2.53e-97 327
MsaG020673 AT1G19220.1 48.454 97 48 2 654 749 953 1048 6.26e-22 102
MsaG020673 AT3G61830.2 50.825 303 134 5 94 396 16 303 5.19e-97 312
MsaG020673 AT3G61830.2 52.000 100 46 2 649 748 429 526 2.91e-24 108
MsaG020673 AT5G20730.2 49.856 347 156 5 54 396 24 356 7.12e-95 321
MsaG020673 AT5G20730.2 46.392 97 50 2 654 749 1032 1127 8.65e-21 99.0
MsaG020673 AT5G20730.3 49.713 348 156 6 54 396 24 357 1.61e-93 317
MsaG020673 AT5G20730.3 46.392 97 50 2 654 749 1033 1128 6.75e-21 99.0
MsaG020673 AT5G20730.1 49.713 348 156 6 54 396 24 357 3.43e-93 316
MsaG020673 AT5G20730.1 46.392 97 50 2 654 749 1033 1128 8.80e-21 98.6
MsaG020673 AT1G35540.1 40.209 383 207 7 49 428 22 385 6.79e-88 290
MsaG020673 AT1G35540.1 52.577 97 45 1 651 747 502 597 5.56e-25 110
MsaG020673 AT1G34310.1 39.024 410 209 9 49 453 22 395 2.99e-86 285
MsaG020673 AT1G34310.1 53.846 91 41 1 651 741 503 592 5.03e-23 104
MsaG020673 AT5G37020.3 54.023 261 118 2 156 415 21 280 3.13e-85 286
MsaG020673 AT5G37020.3 46.667 90 46 2 659 747 610 698 1.20e-18 91.3
MsaG020673 AT1G34170.1 40.984 366 194 8 52 414 23 369 4.16e-85 280
MsaG020673 AT1G35240.1 41.429 350 184 6 49 396 22 352 4.63e-84 279
MsaG020673 AT1G35240.1 54.737 95 42 1 651 745 489 582 3.56e-24 108
MsaG020673 AT1G34410.1 39.840 374 194 7 49 420 22 366 6.93e-84 279
MsaG020673 AT1G34410.1 32.593 270 129 9 491 745 365 596 9.16e-25 110
MsaG020673 AT1G35520.1 40.270 370 198 7 49 416 22 370 1.10e-81 273
MsaG020673 AT1G35520.1 50.549 91 44 1 651 741 503 592 2.68e-22 102
MsaG020673 AT2G28350.2 40.796 402 184 8 54 413 10 399 6.77e-81 273
MsaG020673 AT2G28350.1 40.796 402 184 8 54 413 10 399 7.54e-81 273
MsaG020673 AT2G46530.2 49.042 261 127 4 158 416 29 285 1.65e-80 268
MsaG020673 AT2G46530.2 40.816 147 53 2 634 747 345 490 1.51e-26 115
MsaG020673 AT4G30080.1 40.201 398 191 7 54 416 19 404 1.17e-79 270
MsaG020673 AT1G34390.1 40.496 363 188 8 49 404 22 363 3.16e-79 266
MsaG020673 AT1G34390.1 31.535 241 116 7 515 741 385 590 4.60e-22 101
MsaG020673 AT1G34170.2 38.525 366 177 8 52 414 23 343 2.68e-73 247
MsaG020673 AT1G34170.3 38.525 366 177 8 52 414 23 343 3.91e-72 246
MsaG020673 AT1G34170.3 40.741 108 56 2 647 747 414 520 8.30e-20 94.4
MsaG020673 AT1G77850.1 32.080 399 219 8 55 418 20 401 4.77e-55 200
MsaG020673 AT1G77850.2 32.080 399 219 8 55 418 20 401 5.19e-55 200
MsaG020673 AT1G43950.1 42.466 219 106 5 49 265 22 222 1.60e-48 171
MsaG020673 AT2G46870.1 35.385 130 80 3 145 271 14 142 2.40e-11 66.2
MsaG020673 AT4G01500.1 38.043 92 54 1 164 255 34 122 2.43e-11 66.2
MsaG020673 AT1G13260.1 35.088 114 70 2 154 263 174 287 7.78e-11 65.1
MsaG020673 AT1G01030.2 36.735 98 61 1 158 255 48 144 8.43e-11 64.7
MsaG020673 AT1G01030.1 36.735 98 61 1 158 255 48 144 9.91e-11 64.7

Find 195 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGTTATGCAGGCTTTA+TGG 0.176843 4:+56864560 MsaT020673.1:CDS
GGTATCTCCAATTGCTTAAA+AGG 0.181566 4:-56860733 None:intergenic
CATCTTCTCCACTTCTTCTT+TGG 0.185315 4:-56865861 None:intergenic
CTTCTTCTTTGGTATATATA+TGG 0.187674 4:-56865850 None:intergenic
TGCAACAGATGAAAGGAAAT+TGG 0.198707 4:-56863200 None:intergenic
AACCAAAAGAATCTTGTTTC+TGG 0.233692 4:+56862616 MsaT020673.1:CDS
GTTTCTGCTTCTGCTTCATT+AGG 0.240616 4:+56864885 MsaT020673.1:CDS
TGTAAACTGAAATCCCTTTC+AGG 0.261732 4:+56864624 MsaT020673.1:CDS
TTACATTACCTCTATAAATA+TGG 0.265982 4:-56862472 None:intergenic
GACATTATCACTCATGAAAT+TGG 0.276302 4:-56864763 None:intergenic
CTTCTTCTTATCTTGAACTA+TGG 0.284809 4:+56860614 MsaT020673.1:CDS
CCCTTGACATTGTTATCATT+TGG 0.287764 4:-56864927 None:intergenic
TGCCATAAGGAATGTGAAAA+AGG 0.290889 4:+56860520 MsaT020673.1:CDS
CACCAGAAACAAGATTCTTT+TGG 0.294480 4:-56862618 None:intergenic
TCCGCGGTTTCTTCAGTCTT+GGG 0.308610 4:-56864012 None:intergenic
TTTCTGCTTCTGCTTCATTA+GGG 0.316596 4:+56864886 MsaT020673.1:CDS
GATTCAGGAAGACCATTTCT+AGG 0.323113 4:-56863151 None:intergenic
AGTGAACTGGAGAAACTATT+TGG 0.334062 4:+56865209 MsaT020673.1:CDS
TGATTACCAACAATTGATTC+AGG 0.338555 4:-56863166 None:intergenic
TCCTGGAGCTTATGCTCATT+TGG 0.345344 4:-56864858 None:intergenic
GTTGATTGGAATAAATGATT+TGG 0.346668 4:+56863629 MsaT020673.1:CDS
TTTGAAGGTTCAATAATAAC+TGG 0.349549 4:-56865935 None:intergenic
GAAGTGGAGAAGATGACATT+TGG 0.355497 4:+56865869 MsaT020673.1:CDS
TGACTTTGTAGGGGTGAAAA+TGG 0.355720 4:+56863094 MsaT020673.1:intron
GCAAATTCCTGCAAACTATT+CGG 0.356320 4:+56864954 MsaT020673.1:CDS
ATTTACAGAACACTTCAAAA+AGG 0.356719 4:+56865009 MsaT020673.1:CDS
AAGTGGAGAAGATGACATTT+GGG 0.357385 4:+56865870 MsaT020673.1:CDS
AACCTCATCATTCTCCTTGT+TGG 0.359424 4:-56861072 None:intergenic
ATAGATGGCCTAAATCAAAA+TGG 0.363531 4:+56863658 MsaT020673.1:CDS
GTGATGCTGTGCTCTTTCTA+AGG 0.367166 4:+56862638 MsaT020673.1:CDS
ACCACAACATTTCCTTTCTT+AGG 0.367433 4:-56860667 None:intergenic
TGATTTCAACATCGGAGCTT+GGG 0.367716 4:+56864653 MsaT020673.1:CDS
GTCCGCGGTTTCTTCAGTCT+TGG 0.371861 4:-56864013 None:intergenic
AGAAAAGAAGTTGGAAGATC+TGG 0.374337 4:+56864822 MsaT020673.1:CDS
CTCCACACCATGCAGGTCTT+TGG 0.378354 4:-56862438 None:intergenic
GAAAACATGAAACATGCTTT+TGG 0.379376 4:-56863236 None:intergenic
ACTATCGATGGTGTGTTAAA+CGG 0.382747 4:-56864786 None:intergenic
AGGCGGCATCTGCTCACAAC+TGG 0.384596 4:+56862577 MsaT020673.1:CDS
CATCAAAACCTGGCATCAAC+TGG 0.390598 4:+56864492 MsaT020673.1:CDS
ATCAAAATGGAGGTGCTTGA+TGG 0.392147 4:+56863671 MsaT020673.1:CDS
TCTTGACCTTGCAAGACCTT+TGG 0.393008 4:-56864387 None:intergenic
AAAGAAGTTGAGGAATTAGA+AGG 0.393061 4:+56861848 MsaT020673.1:CDS
CTTCTTAGAGATTCTGATAA+GGG 0.397025 4:+56865242 MsaT020673.1:CDS
CTATCGATGGTGTGTTAAAC+GGG 0.397418 4:-56864785 None:intergenic
TCAAGAATCCAGTGAGTATA+GGG 0.398915 4:+56863458 MsaT020673.1:CDS
CACAAGCAAGGAAGCTTAGT+TGG 0.408419 4:+56865140 MsaT020673.1:CDS
CTATTCGGCTTTCCTTTGTC+TGG 0.408734 4:+56864969 MsaT020673.1:CDS
GTTCAAAATGAGAATTGAAA+TGG 0.409109 4:+56863486 MsaT020673.1:CDS
TGAAAATGGAGAGCTGAGAT+TGG 0.413215 4:+56863108 MsaT020673.1:CDS
GCAACAGATGAAAGGAAATT+GGG 0.413332 4:-56863199 None:intergenic
TTTGATTTAGGCCATCTATA+GGG 0.413502 4:-56863654 None:intergenic
CCAACTGAGGAAGACTTTGA+AGG 0.413561 4:-56865950 None:intergenic
GTTAATCCTTTCAGTTATGC+AGG 0.425087 4:+56864552 MsaT020673.1:CDS
GGAGATGAAGAGGGAGATGG+AGG 0.430363 4:+56861869 MsaT020673.1:CDS
ACTACTGTTGGAGAATAATC+TGG 0.435153 4:-56865977 None:intergenic
TCAGTTGGCAGGGATGCATA+TGG 0.435408 4:+56861820 MsaT020673.1:intron
AGCAGGAATCAGTGGAATGA+TGG 0.437544 4:+56864341 MsaT020673.1:intron
TTCCTATTCCAGTTGATGCC+AGG 0.437834 4:-56864500 None:intergenic
TGAGGCTGAAGATCATAGTT+TGG 0.438101 4:-56860754 None:intergenic
CTGATTTCAACATCGGAGCT+TGG 0.438282 4:+56864652 MsaT020673.1:CDS
TGGGGAAGACTGAATGCTTA+GGG 0.438392 4:-56863993 None:intergenic
CAGTGGAATGATGGGCTTTG+AGG 0.440411 4:+56864350 MsaT020673.1:CDS
AGGAAAGCCGAATAGTTTGC+AGG 0.443064 4:-56864961 None:intergenic
ATGATTTGGATCCCTATAGA+TGG 0.446543 4:+56863643 MsaT020673.1:CDS
TGATGATACTCATAGCTGTT+TGG 0.448093 4:+56865904 MsaT020673.1:CDS
CAATGATCTTCTGAGTGAAC+TGG 0.449039 4:+56865196 MsaT020673.1:CDS
GGTCTTCCTGAATCAATTGT+TGG 0.449503 4:+56863160 MsaT020673.1:CDS
CTTGAAGATGAACAAGTTGA+AGG 0.457374 4:-56860571 None:intergenic
TACTCATAGCTGTTTGGATC+AGG 0.457597 4:+56865910 MsaT020673.1:CDS
CTTTAGACTCTAACTACTGT+TGG 0.457649 4:-56865989 None:intergenic
TTGGGGAAGACTGAATGCTT+AGG 0.458895 4:-56863994 None:intergenic
GAAGGAGATGAAGAGGGAGA+TGG 0.463926 4:+56861866 MsaT020673.1:CDS
AACCTTGTCCCTATACTCAC+TGG 0.465299 4:-56863466 None:intergenic
AACCTGGCATCAACTGGAAT+AGG 0.466535 4:+56864498 MsaT020673.1:CDS
CGATCTCCCATGGCGATACT+CGG 0.468600 4:-56863949 None:intergenic
GGAATTAGAAGGAGATGAAG+AGG 0.472885 4:+56861859 MsaT020673.1:CDS
ACTTCTTAGAGATTCTGATA+AGG 0.476305 4:+56865241 MsaT020673.1:CDS
GTGACATACAACAGGTAAGA+TGG 0.476656 4:+56863897 MsaT020673.1:intron
GGCATCTGCTCACAACTGGC+TGG 0.479273 4:+56862581 MsaT020673.1:CDS
ATCAAGAATCCAGTGAGTAT+AGG 0.479733 4:+56863457 MsaT020673.1:CDS
GAACTATGGCATGCTTGTGC+TGG 0.483587 4:+56860628 MsaT020673.1:CDS
TGTAGTAGTGGTATGTTGAT+TGG 0.485540 4:+56863615 MsaT020673.1:CDS
AAACATGCAGATTGAAAACT+AGG 0.485809 4:-56864684 None:intergenic
GAAACCGCGGACCGGTCTAC+TGG 0.486331 4:+56864024 MsaT020673.1:CDS
TGGAAAGTAACAACTTTCCA+AGG 0.487409 4:+56864580 MsaT020673.1:CDS
AAATTTCGCCATATTTATAG+AGG 0.488170 4:+56862464 MsaT020673.1:CDS
TCTTGACCTTGCAAGACCCT+TGG 0.488979 4:-56864597 None:intergenic
TTCTTAGGAAGTGAGGTAAG+AGG 0.495941 4:-56860652 None:intergenic
GCTTTGTCAATATCTCTGTA+AGG 0.495987 4:-56864708 None:intergenic
TTGCAAGGTCAAGAAAATAC+AGG 0.496208 4:+56864396 MsaT020673.1:CDS
CCTTCAAAGTCTTCCTCAGT+TGG 0.496667 4:+56865950 MsaT020673.1:CDS
CATACCAGTGATGAGGTGAT+TGG 0.496922 4:-56864056 None:intergenic
GAAGAATGCATTGTGCCATA+AGG 0.497724 4:+56860507 MsaT020673.1:CDS
GTCTCTGATACCAGCACTCA+TGG 0.499971 4:+56861938 MsaT020673.1:CDS
TACTCTGCTACCTCAGGCTG+AGG 0.501022 4:+56861106 MsaT020673.1:CDS
AGAGCTGTTAGACCTAGAAA+TGG 0.501621 4:+56863139 MsaT020673.1:CDS
TGCAGATTGAAAACTAGGCT+TGG 0.506257 4:-56864679 None:intergenic
CTTACCTCACTTCCTAAGAA+AGG 0.508886 4:+56860655 MsaT020673.1:CDS
GAAAATGGAGAGCTGAGATT+GGG 0.510036 4:+56863109 MsaT020673.1:CDS
AACACACCATCGATAGTGGC+AGG 0.510410 4:+56864792 MsaT020673.1:CDS
CATTCTCACTGTCAGTGTAA+AGG 0.513773 4:-56865274 None:intergenic
TATATATACCAAAGAAGAAG+TGG 0.514232 4:+56865853 MsaT020673.1:CDS
TGCTCAACTCAAAACCTGGC+TGG 0.515074 4:-56864458 None:intergenic
CCGCGGTTTCTTCAGTCTTG+GGG 0.515903 4:-56864011 None:intergenic
GGGAACATTAGAAAAGAAGT+TGG 0.522575 4:+56864813 MsaT020673.1:CDS
GTTTAACACACCATCGATAG+TGG 0.526144 4:+56864788 MsaT020673.1:CDS
ATTGAGTACTCACAAGTAGC+TGG 0.528164 4:-56860801 None:intergenic
GAATTAGAAGGAGATGAAGA+GGG 0.531417 4:+56861860 MsaT020673.1:CDS
TCCAAATGAGCATAAGCTCC+AGG 0.532461 4:+56864857 MsaT020673.1:CDS
GTTGTGAGCAGATGCCGCCT+TGG 0.534028 4:-56862574 None:intergenic
ACAGCTATGAGTATCATCAT+TGG 0.534267 4:-56865900 None:intergenic
TGAGAATGACATAATGGTTG+TGG 0.539248 4:+56865289 MsaT020673.1:CDS
TCCAAATGATAACAATGTCA+AGG 0.539957 4:+56864926 MsaT020673.1:CDS
CAACTCAAAACCTGGCTGGT+TGG 0.540058 4:-56864454 None:intergenic
AATTGTTCTAAGTGCCCTTG+TGG 0.541589 4:-56860697 None:intergenic
TGGATGAATCACCAGAAAGA+AGG 0.543057 4:+56863506 MsaT020673.1:CDS
TGAATGCTTAGGGGAGGCTG+AGG 0.543172 4:-56863983 None:intergenic
TCACCCAATCACCTCATCAC+TGG 0.544643 4:+56864052 MsaT020673.1:CDS
GGAGAATCCACCATGAGTGC+TGG 0.545318 4:-56861948 None:intergenic
TTAGAGATTCTGATAAGGGA+TGG 0.545399 4:+56865246 MsaT020673.1:CDS
GCAGGAATCAGTGGAATGAT+GGG 0.546322 4:+56864342 MsaT020673.1:intron
TTCTGCTTCTGCTTCATTAG+GGG 0.547616 4:+56864887 MsaT020673.1:CDS
GAAGAGCCATTGATCTCTCT+AGG 0.548597 4:+56865162 MsaT020673.1:CDS
CAGAAGCAGAAACATTGTCC+TGG 0.550901 4:-56864875 None:intergenic
TTTGGCAACAAGCTCTTGAG+AGG 0.551407 4:-56862420 None:intergenic
AAGAAGAATACTTACTGCCA+TGG 0.552627 4:-56865320 None:intergenic
GGAGCTTATGCTCATTTGGA+AGG 0.553078 4:-56864854 None:intergenic
TCTAAACAATGAAGTGATTG+AGG 0.553452 4:+56860480 MsaT020673.1:CDS
TGACAGTGAGAATGACATAA+TGG 0.554268 4:+56865283 MsaT020673.1:CDS
ATTTCCTTTCTTAGGAAGTG+AGG 0.560818 4:-56860659 None:intergenic
GGAAAAGCTGATTTCAACAT+CGG 0.565551 4:+56864645 MsaT020673.1:CDS
TGACATACAACAGGTAAGAT+GGG 0.566150 4:+56863898 MsaT020673.1:intron
GAGAATGACATAATGGTTGT+GGG 0.567615 4:+56865290 MsaT020673.1:CDS
CTATCTTGTAGGTGTAGTAG+TGG 0.570573 4:+56863603 MsaT020673.1:intron
ATACCAGTGATGAGGTGATT+GGG 0.571375 4:-56864055 None:intergenic
ATGTTCCCTGCCACTATCGA+TGG 0.572030 4:-56864798 None:intergenic
ACAAGTTACTCTGCTACCTC+AGG 0.572482 4:+56861100 MsaT020673.1:CDS
GAGATGAAGAGGGAGATGGA+GGG 0.575170 4:+56861870 MsaT020673.1:CDS
TAGCATTTGCAACAGATGAA+AGG 0.577301 4:-56863207 None:intergenic
ATAAAGCCTGCATAACTGAA+AGG 0.578447 4:-56864558 None:intergenic
CCAAATGATAACAATGTCAA+GGG 0.578863 4:+56864927 MsaT020673.1:CDS
GATTTCAACATCGGAGCTTG+GGG 0.582139 4:+56864654 MsaT020673.1:CDS
CAAATGAAGGTTCACAAGCA+AGG 0.586098 4:+56865128 MsaT020673.1:intron
TTCAGCAGCTCTACGAGGCA+CGG 0.586620 4:-56861969 None:intergenic
GACAAAGTAACCAACCAGCC+AGG 0.589462 4:+56864444 MsaT020673.1:CDS
TTTATGGCAGCAGGAATCAG+TGG 0.590392 4:+56864333 MsaT020673.1:intron
ATCAAGACCGAGTATCGCCA+TGG 0.592676 4:+56863942 MsaT020673.1:CDS
ATTTCCACTCCACACCATGC+AGG 0.596067 4:-56862445 None:intergenic
AACTTTCCAAGGGTCTTGCA+AGG 0.597137 4:+56864591 MsaT020673.1:CDS
TATGGAAGGAAAAGAAGTTG+AGG 0.598443 4:+56861838 MsaT020673.1:CDS
ACTGGAGAAACTATTTGGCA+TGG 0.598525 4:+56865214 MsaT020673.1:CDS
AGACTGAAGAAACCGCGGAC+CGG 0.598571 4:+56864016 MsaT020673.1:CDS
GAGAAACTATTTGGCATGGA+AGG 0.600086 4:+56865218 MsaT020673.1:CDS
TTCAAGTCATCATCAAAACC+TGG 0.600469 4:+56864482 MsaT020673.1:CDS
GGGTGAGGTAACCAGTAGAC+CGG 0.601618 4:-56864035 None:intergenic
CTAATTTGTCAGGTCAGCCA+AGG 0.606026 4:+56862557 MsaT020673.1:intron
GTGTCTTGCAGAACATGTGA+GGG 0.607798 4:-56861910 None:intergenic
GAAGAAAGATCGATCTCCCA+TGG 0.608121 4:-56863959 None:intergenic
CTACTAGGTTCGCCAGACAA+AGG 0.611679 4:-56864981 None:intergenic
ACAACAGGTAAGATGGGATG+AGG 0.612245 4:+56863904 MsaT020673.1:intron
GAAGTGCTCAACTCAAAACC+TGG 0.615230 4:-56864462 None:intergenic
AGTGTCTTGCAGAACATGTG+AGG 0.616842 4:-56861911 None:intergenic
GGAAAGTAACAACTTTCCAA+GGG 0.618100 4:+56864581 MsaT020673.1:CDS
AGATCACCAAAGGTCTTGCA+AGG 0.621574 4:+56864381 MsaT020673.1:CDS
ATAATCTGGCTGACCAACTG+AGG 0.623428 4:-56865963 None:intergenic
GCTCAACCTAGAGAGATCAA+TGG 0.626281 4:-56865168 None:intergenic
AAGACCTGCATGGTGTGGAG+TGG 0.629896 4:+56862441 MsaT020673.1:CDS
GAGATTCTGATAAGGGATGG+AGG 0.631623 4:+56865249 MsaT020673.1:CDS
TGCCAAAGACCTGCATGGTG+TGG 0.632330 4:+56862436 MsaT020673.1:CDS
TTGGCAACAAGCTCTTGAGA+GGG 0.634142 4:-56862419 None:intergenic
AGTGATGAGGTGATTGGGTG+AGG 0.637047 4:-56864050 None:intergenic
GGGGAAGACTGAATGCTTAG+GGG 0.643082 4:-56863992 None:intergenic
ACAACCCTGCAAAAGATCTG+AGG 0.644320 4:-56860772 None:intergenic
TTGGCAGGGATGCATATGGA+AGG 0.645626 4:+56861824 MsaT020673.1:CDS
TCCTAAGAAAGGAAATGTTG+TGG 0.646376 4:+56860666 MsaT020673.1:CDS
TAGGATTCATACCAGTGATG+AGG 0.646397 4:-56864063 None:intergenic
ATCCAGTGAGTATAGGGACA+AGG 0.648334 4:+56863464 MsaT020673.1:CDS
AGATAAGAAGAAGAAACAAG+TGG 0.649698 4:-56860604 None:intergenic
ATTGATCTCTCTAGGTTGAG+CGG 0.652349 4:+56865170 MsaT020673.1:CDS
GATGGCCTAAATCAAAATGG+AGG 0.658795 4:+56863661 MsaT020673.1:CDS
GGAGTCAGTAAGATCACCAA+AGG 0.663614 4:+56864371 MsaT020673.1:CDS
TCAAGACCGAGTATCGCCAT+GGG 0.667440 4:+56863943 MsaT020673.1:CDS
ACACACCATCGATAGTGGCA+GGG 0.674307 4:+56864793 MsaT020673.1:CDS
GAAGACTGAATGCTTAGGGG+AGG 0.675841 4:-56863989 None:intergenic
GAATGACATAATGGTTGTGG+GGG 0.679524 4:+56865292 MsaT020673.1:CDS
AGAATGACATAATGGTTGTG+GGG 0.682622 4:+56865291 MsaT020673.1:CDS
TCTGATACCAGCACTCATGG+TGG 0.683675 4:+56861941 MsaT020673.1:CDS
ATTTGTCAGGTCAGCCAAGG+CGG 0.683923 4:+56862560 MsaT020673.1:intron
GGCCAACAAGGAGAATGATG+AGG 0.688468 4:+56861070 MsaT020673.1:intron
CCCCAAGACTGAAGAAACCG+CGG 0.689701 4:+56864011 MsaT020673.1:CDS
CAATCTTCAGCAGCTCTACG+AGG 0.704658 4:-56861974 None:intergenic
CTTGTTGCCAAAGACCTGCA+TGG 0.711328 4:+56862431 MsaT020673.1:CDS
ATTAACACTCATAAGCTCAG+AGG 0.715454 4:-56864535 None:intergenic
GTAACCAGTAGACCGGTCCG+CGG 0.729539 4:-56864028 None:intergenic
TGTCTTGCAGAACATGTGAG+GGG 0.734062 4:-56861909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 56860469 56866009 56860469 ID=MsaG020673
Chr4 mRNA 56860469 56866009 56860469 ID=MsaT020673.1;Parent=MsaG020673
Chr4 exon 56860469 56860810 56860469 ID=MsaT020673.1.exon1;Parent=MsaT020673.1
Chr4 CDS 56860469 56860810 56860469 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56861071 56861127 56861071 ID=MsaT020673.1.exon2;Parent=MsaT020673.1
Chr4 CDS 56861071 56861127 56861071 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56861824 56862009 56861824 ID=MsaT020673.1.exon3;Parent=MsaT020673.1
Chr4 CDS 56861824 56862009 56861824 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56862401 56862485 56862401 ID=MsaT020673.1.exon4;Parent=MsaT020673.1
Chr4 CDS 56862401 56862485 56862401 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56862569 56862659 56862569 ID=MsaT020673.1.exon5;Parent=MsaT020673.1
Chr4 CDS 56862569 56862659 56862569 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56863105 56863269 56863105 ID=MsaT020673.1.exon6;Parent=MsaT020673.1
Chr4 CDS 56863105 56863269 56863105 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56863408 56863527 56863408 ID=MsaT020673.1.exon7;Parent=MsaT020673.1
Chr4 CDS 56863408 56863527 56863408 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56863614 56863692 56863614 ID=MsaT020673.1.exon8;Parent=MsaT020673.1
Chr4 CDS 56863614 56863692 56863614 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56863911 56864073 56863911 ID=MsaT020673.1.exon9;Parent=MsaT020673.1
Chr4 CDS 56863911 56864073 56863911 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56864343 56865043 56864343 ID=MsaT020673.1.exon10;Parent=MsaT020673.1
Chr4 CDS 56864343 56865043 56864343 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56865137 56865327 56865137 ID=MsaT020673.1.exon11;Parent=MsaT020673.1
Chr4 CDS 56865137 56865327 56865137 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Chr4 exon 56865823 56866009 56865823 ID=MsaT020673.1.exon12;Parent=MsaT020673.1
Chr4 CDS 56865823 56866009 56865823 ID=cds.MsaT020673.1;Parent=MsaT020673.1
Gene Sequence

>MsaT020673.1

ATGGAAATTGATCTAAACAATGAAGTGATTGAGGTTGAGAAGAATGCATTGTGCCATAAGGAATGTGAAAAAGGTTTTTGTTTCTGTATTTCTTGTTTGTCTCCTTCAACTTGTTCATCTTCAAGTTCAACTTCACCACTTGTTTCTTCTTCTTATCTTGAACTATGGCATGCTTGTGCTGGTCCTCTTACCTCACTTCCTAAGAAAGGAAATGTTGTGGTGTATTTTCCACAAGGGCACTTAGAACAATTTTCTTCTTTCTCTCCTTTTAAGCAATTGGAGATACCAAACTATGATCTTCAGCCTCAGATCTTTTGCAGGGTTGTCAATGTCCAGCTACTTGCCAACAAGGAGAATGATGAGGTTTATACACAAGTTACTCTGCTACCTCAGGCTGAGTTGGCAGGGATGCATATGGAAGGAAAAGAAGTTGAGGAATTAGAAGGAGATGAAGAGGGAGATGGAGGGTCACCTACAAAATCAACCCCTCACATGTTCTGCAAGACACTAACTGTCTCTGATACCAGCACTCATGGTGGATTCTCCGTGCCTCGTAGAGCTGCTGAAGATTGTTTTCCTCCATTGGATTATAAGCTGCAGAGACCCTCTCAAGAGCTTGTTGCCAAAGACCTGCATGGTGTGGAGTGGAAATTTCGCCATATTTATAGAGGTCAGCCAAGGCGGCATCTGCTCACAACTGGCTGGAGCATTTTTGTGAACCAAAAGAATCTTGTTTCTGGTGATGCTGTGCTCTTTCTAAGGGGTGAAAATGGAGAGCTGAGATTGGGAATCAGAAGAGCTGTTAGACCTAGAAATGGTCTTCCTGAATCAATTGTTGGTAATCAGAATTGTTATCCCAATTTCCTTTCATCTGTTGCAAATGCTATATCCACCAAAAGCATGTTTCATGTTTTCTATAGTCCAAGAGCAAGTCATGCAGAGTTTGTTGTTCCGTACCAAAAATATGTTAAAAGCATCAAGAATCCAGTGAGTATAGGGACAAGGTTCAAAATGAGAATTGAAATGGATGAATCACCAGAAAGAAGGTGTAGTAGTGGTATGTTGATTGGAATAAATGATTTGGATCCCTATAGATGGCCTAAATCAAAATGGAGGTGCTTGATGGTAAGATGGGATGAGGATACTGAGACTAATCATCAAGACCGAGTATCGCCATGGGAGATCGATCTTTCTTCTCCTCAGCCTCCCCTAAGCATTCAGTCTTCCCCAAGACTGAAGAAACCGCGGACCGGTCTACTGGTTACCTCACCCAATCACCTCATCACTGCAGGAATCAGTGGAATGATGGGCTTTGAGGAGTCAGTAAGATCACCAAAGGTCTTGCAAGGTCAAGAAAATACAGGTTTTATGTCACTATACTATGGATGTGACAAAGTAACCAACCAGCCAGGTTTTGAGTTGAGCACTTCAAGTCATCATCAAAACCTGGCATCAACTGGAATAGGAAAAGTAGTTACATCCTCTGAGCTTATGAGTGTTAATCCTTTCAGTTATGCAGGCTTTATGGAAAGTAACAACTTTCCAAGGGTCTTGCAAGGTCAAGAAATATGTAAACTGAAATCCCTTTCAGGAAAAGCTGATTTCAACATCGGAGCTTGGGGAACCAAGCCTAGTTTTCAATCTGCATGTTTTCCTTACAGAGATATTGACAAAGCTAATCAAGCTAATATTTTTAGCTCAAAACACACCAATTTCATGAGTGATAATGTCCCGTTTAACACACCATCGATAGTGGCAGGGAACATTAGAAAAGAAGTTGGAAGATCTGGATCTAATCTCCTTCCAAATGAGCATAAGCTCCAGGACAATGTTTCTGCTTCTGCTTCATTAGGGGATACTAATATTAAAGTTCCAAATGATAACAATGTCAAGGGAAATGCAAATTCCTGCAAACTATTCGGCTTTCCTTTGTCTGGCGAACCTAGTAGTCAAAATTTACAGAACACTTCAAAAAGGAGTTGCACAAAGGTTCACAAGCAAGGAAGCTTAGTTGGAAGAGCCATTGATCTCTCTAGGTTGAGCGGCTACAATGATCTTCTGAGTGAACTGGAGAAACTATTTGGCATGGAAGGACTTCTTAGAGATTCTGATAAGGGATGGAGGATCCTTTACACTGACAGTGAGAATGACATAATGGTTGTGGGGGATGATCCATGGCATGAGTTTTGTGATGTGGTGTCTAAAATCCATATATATACCAAAGAAGAAGTGGAGAAGATGACATTTGGGATGATGACCAATGATGATACTCATAGCTGTTTGGATCAGGCACCAGTTATTATTGAACCTTCAAAGTCTTCCTCAGTTGGTCAGCCAGATTATTCTCCAACAGTAGTTAGAGTCTAA
Protein sequence

>MsaT020673.1

MEIDLNNEVIEVEKNALCHKECEKGFCFCISCLSPSTCSSSSSTSPLVSSSYLELWHACAGPLTSLPKKGNVVVYFPQGHLEQFSSFSPFKQLEIPNYDLQPQIFCRVVNVQLLANKENDEVYTQVTLLPQAELAGMHMEGKEVEELEGDEEGDGGSPTKSTPHMFCKTLTVSDTSTHGGFSVPRRAAEDCFPPLDYKLQRPSQELVAKDLHGVEWKFRHIYRGQPRRHLLTTGWSIFVNQKNLVSGDAVLFLRGENGELRLGIRRAVRPRNGLPESIVGNQNCYPNFLSSVANAISTKSMFHVFYSPRASHAEFVVPYQKYVKSIKNPVSIGTRFKMRIEMDESPERRCSSGMLIGINDLDPYRWPKSKWRCLMVRWDEDTETNHQDRVSPWEIDLSSPQPPLSIQSSPRLKKPRTGLLVTSPNHLITAGISGMMGFEESVRSPKVLQGQENTGFMSLYYGCDKVTNQPGFELSTSSHHQNLASTGIGKVVTSSELMSVNPFSYAGFMESNNFPRVLQGQEICKLKSLSGKADFNIGAWGTKPSFQSACFPYRDIDKANQANIFSSKHTNFMSDNVPFNTPSIVAGNIRKEVGRSGSNLLPNEHKLQDNVSASASLGDTNIKVPNDNNVKGNANSCKLFGFPLSGEPSSQNLQNTSKRSCTKVHKQGSLVGRAIDLSRLSGYNDLLSELEKLFGMEGLLRDSDKGWRILYTDSENDIMVVGDDPWHEFCDVVSKIHIYTKEEVEKMTFGMMTNDDTHSCLDQAPVIIEPSKSSSVGQPDYSPTVVRV*