AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020695


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020695 MtrunA17_Chr4g0030051 95.402 174 8 0 1 174 1 174 1.18e-101 295
MsaG020695 MtrunA17_Chr4g0030051 100.000 41 0 0 167 207 249 289 9.43e-23 92.8
MsaG020695 MtrunA17_Chr2g0326001 68.553 159 38 6 19 168 18 173 1.36e-60 191
MsaG020695 MtrunA17_Chr3g0117601 52.027 148 47 5 2 141 28 159 1.30e-35 127
MsaG020695 MtrunA17_Chr3g0127201 76.471 68 16 0 30 97 16 83 1.79e-34 124
MsaG020695 MtrunA17_Chr1g0188851 56.701 97 38 1 3 95 1 97 2.97e-34 123
MsaG020695 MtrunA17_Chr4g0031411 66.304 92 26 2 25 111 58 149 5.84e-34 123
MsaG020695 MtrunA17_Chr8g0341671 70.270 74 22 0 29 102 8 81 1.23e-32 117
MsaG020695 MtrunA17_Chr2g0328021 60.000 85 34 0 12 96 33 117 2.30e-32 118
MsaG020695 MtrunA17_Chr3g0126201 64.706 85 30 0 15 99 25 109 2.59e-32 119
MsaG020695 MtrunA17_Chr4g0010151 66.250 80 26 1 17 95 57 136 3.58e-32 119
MsaG020695 MtrunA17_Chr4g0047001 59.596 99 30 2 42 130 56 154 7.67e-32 119
MsaG020695 MtrunA17_Chr8g0346551 66.234 77 24 1 23 99 8 82 8.62e-32 116
MsaG020695 MtrunA17_Chr1g0177251 64.368 87 27 2 43 125 14 100 2.24e-31 115
MsaG020695 MtrunA17_Chr7g0237921 68.831 77 23 1 20 95 54 130 2.94e-31 116
MsaG020695 MtrunA17_Chr2g0282441 85.965 57 6 1 36 92 22 76 6.40e-31 115
MsaG020695 MtrunA17_Chr8g0374251 52.294 109 37 3 39 147 66 159 6.64e-31 115
MsaG020695 MtrunA17_Chr8g0367161 58.333 84 35 0 30 113 21 104 8.08e-31 115
MsaG020695 MtrunA17_Chr7g0223311 72.857 70 18 1 40 108 76 145 1.02e-29 113
MsaG020695 MtrunA17_Chr4g0061761 86.792 53 7 0 39 91 38 90 1.15e-29 112
MsaG020695 MtrunA17_Chr8g0374111 70.588 68 18 1 32 97 18 85 1.50e-29 109
MsaG020695 MtrunA17_Chr5g0411951 80.702 57 10 1 35 91 38 93 1.62e-29 112
MsaG020695 MtrunA17_Chr2g0282511 90.000 50 5 0 40 89 44 93 1.89e-28 108
MsaG020695 MtrunA17_Chr2g0282051 60.465 86 31 2 10 95 17 99 5.73e-28 106
MsaG020695 MtrunA17_Chr2g0308011 88.000 50 6 0 40 89 36 85 9.98e-28 104
MsaG020695 MtrunA17_Chr4g0047761 80.702 57 10 1 39 95 70 125 5.80e-27 104
MsaG020695 MtrunA17_Chr3g0092111 66.197 71 23 1 29 99 125 194 1.98e-26 105
MsaG020695 MtrunA17_Chr7g0217581 67.143 70 22 1 29 98 221 289 2.74e-26 105
MsaG020695 MtrunA17_Chr6g0460731 51.042 96 45 2 4 98 59 153 4.32e-26 103
MsaG020695 MtrunA17_Chr5g0417571 59.740 77 30 1 29 105 127 202 9.97e-26 101
MsaG020695 MtrunA17_Chr6g0460721 46.809 94 49 1 5 98 54 146 1.06e-25 101
MsaG020695 MtrunA17_Chr6g0453321 52.174 92 43 1 7 98 103 193 6.38e-25 101
MsaG020695 MtrunA17_Chr7g0254161 59.722 72 28 1 27 98 88 158 1.44e-24 99.8
MsaG020695 MtrunA17_Chr4g0042481 54.321 81 33 1 19 99 104 180 2.04e-24 99.8
MsaG020695 MtrunA17_Chr8g0355261 47.059 102 40 3 29 128 107 196 6.07e-24 98.2
MsaG020695 MtrunA17_Chr2g0283811 67.857 56 18 0 39 94 45 100 2.33e-23 91.7
MsaG020695 MtrunA17_Chr5g0417551 59.211 76 27 1 25 100 115 186 1.59e-22 93.6
MsaG020695 MtrunA17_Chr5g0417561 59.211 76 27 1 25 100 107 178 2.49e-22 92.8
MsaG020695 MtrunA17_Chr5g0417601 58.571 70 28 1 25 94 125 193 1.96e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020695 AT2G28510.1 51.977 177 56 7 10 157 4 180 6.46e-43 145
MsaG020695 AT5G60200.1 45.263 190 71 6 25 182 40 228 1.69e-42 144
MsaG020695 AT3G45610.1 50.314 159 64 5 3 149 1 156 3.47e-39 135
MsaG020695 AT1G51700.1 63.158 95 31 2 21 114 16 107 2.66e-35 123
MsaG020695 AT3G55370.3 71.429 77 21 1 25 100 104 180 2.14e-33 123
MsaG020695 AT3G55370.1 71.429 77 21 1 25 100 59 135 2.79e-33 122
MsaG020695 AT3G55370.2 71.429 77 21 1 25 100 59 135 3.85e-33 122
MsaG020695 AT5G02460.1 73.611 72 18 1 25 95 78 149 2.09e-32 120
MsaG020695 AT1G21340.1 65.116 86 18 1 40 113 37 122 4.88e-32 117
MsaG020695 AT4G24060.1 72.727 66 18 0 30 95 42 107 5.39e-32 119
MsaG020695 AT1G07640.2 65.823 79 26 1 18 95 53 131 5.75e-32 118
MsaG020695 AT1G07640.3 65.823 79 26 1 18 95 61 139 7.78e-32 118
MsaG020695 AT3G52440.1 59.574 94 33 1 43 136 27 115 8.31e-32 116
MsaG020695 AT5G60850.1 72.000 75 19 1 39 111 49 123 9.91e-32 117
MsaG020695 AT3G52440.2 59.574 94 33 1 43 136 46 134 1.11e-31 116
MsaG020695 AT3G61850.2 52.066 121 52 3 27 147 49 163 1.17e-31 116
MsaG020695 AT3G61850.3 52.066 121 52 3 27 147 49 163 1.17e-31 116
MsaG020695 AT3G61850.1 52.066 121 52 3 27 147 61 175 1.38e-31 116
MsaG020695 AT2G37590.1 67.123 73 23 1 25 96 72 144 1.39e-31 117
MsaG020695 AT3G61850.5 52.066 121 52 3 27 147 49 163 1.61e-31 117
MsaG020695 AT3G61850.4 52.066 121 52 3 27 147 61 175 1.88e-31 117
MsaG020695 AT1G07640.1 69.444 72 21 1 25 95 4 75 2.40e-31 115
MsaG020695 AT2G28810.2 69.565 69 20 1 24 92 61 128 3.65e-31 116
MsaG020695 AT2G28810.1 69.565 69 20 1 24 92 78 145 5.85e-31 116
MsaG020695 AT5G62940.1 77.049 61 14 0 31 91 63 123 1.02e-30 115
MsaG020695 AT5G65590.1 80.328 61 12 0 41 101 41 101 1.23e-30 114
MsaG020695 AT1G28310.1 87.273 55 7 0 41 95 27 81 3.49e-30 113
MsaG020695 AT1G28310.2 58.947 95 39 0 1 95 11 105 5.03e-30 113
MsaG020695 AT3G21270.1 90.566 53 5 0 41 93 29 81 1.50e-29 109
MsaG020695 AT4G38000.1 72.727 66 17 1 30 94 30 95 3.89e-29 109
MsaG020695 AT4G21050.1 63.158 76 28 0 38 113 21 96 5.59e-29 107
MsaG020695 AT3G50410.1 72.308 65 18 0 33 97 22 86 8.39e-29 108
MsaG020695 AT4G00940.1 80.702 57 11 0 39 95 66 122 2.09e-28 108
MsaG020695 AT4G00940.3 80.702 57 11 0 39 95 66 122 2.09e-28 108
MsaG020695 AT4G00940.2 80.702 57 11 0 39 95 66 122 2.09e-28 108
MsaG020695 AT5G66940.1 61.905 84 25 2 37 113 28 111 2.13e-28 106
MsaG020695 AT1G64620.1 72.131 61 17 0 30 90 38 98 6.68e-28 108
MsaG020695 AT2G46590.1 63.158 76 24 1 13 88 44 115 1.09e-27 107
MsaG020695 AT2G46590.2 63.158 76 24 1 13 88 56 127 1.27e-27 107
MsaG020695 AT1G47655.1 75.439 57 14 0 33 89 21 77 2.22e-26 101
MsaG020695 AT5G62430.1 47.959 98 45 2 3 100 22 113 3.58e-26 102
MsaG020695 AT3G47500.1 52.632 95 41 1 4 98 77 167 5.43e-26 104
MsaG020695 AT5G39660.3 61.333 75 25 1 24 98 125 195 8.06e-25 101
MsaG020695 AT5G39660.1 61.333 75 25 1 24 98 125 195 8.06e-25 101
MsaG020695 AT5G39660.2 61.333 75 25 1 24 98 125 195 8.06e-25 101
MsaG020695 AT1G69570.1 53.488 86 36 1 9 94 104 185 1.51e-24 99.8
MsaG020695 AT4G21080.1 56.579 76 24 1 43 109 27 102 2.05e-24 97.1
MsaG020695 AT1G26790.1 55.263 76 33 1 20 94 89 164 5.68e-24 97.8
MsaG020695 AT4G21040.1 75.000 52 13 0 43 94 27 78 8.70e-24 94.7
MsaG020695 AT2G34140.1 66.071 56 19 0 39 94 56 111 4.67e-23 91.3
MsaG020695 AT1G29160.1 66.071 56 19 0 39 94 60 115 8.79e-23 90.9
MsaG020695 AT4G21030.1 51.471 68 33 0 38 105 18 85 1.63e-15 72.0

Find 57 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTTTGGTTTCCTAAAA+TGG 0.139828 4:+57281166 None:intergenic
GGGAAACAAGTGAGTGGATT+TGG 0.231178 4:+57281278 None:intergenic
GCTTCTGGTTGAGGAGGTTT+TGG 0.248273 4:+57281518 None:intergenic
GCAAGTCTTGTAGGAGATAT+TGG 0.255429 4:-57281412 MsaT020695.1:CDS
GAATGGTCTCACTTCATCTT+GGG 0.274434 4:-57280799 MsaT020695.1:CDS
GATATTTCTGCAAGTCTTGT+AGG 0.294030 4:-57281421 MsaT020695.1:CDS
GGAATGGTCTCACTTCATCT+TGG 0.324184 4:-57280800 MsaT020695.1:CDS
ATTGTTGTAGTAACAAAATT+TGG 0.325870 4:+57281463 None:intergenic
TTTCCATGGCAGTTCAATAA+TGG 0.330980 4:-57280867 MsaT020695.1:CDS
GGAAACAAGTGAGTGGATTT+GGG 0.349121 4:+57281279 None:intergenic
ACGAGACGGTGTCTACATTA+GGG 0.358019 4:+57280755 None:intergenic
ATGGCTGAAATGGATCATTT+AGG 0.361202 4:-57280834 MsaT020695.1:CDS
AGACACAAATATGGCTGAAA+TGG 0.367887 4:-57280844 MsaT020695.1:CDS
CACGAGACGGTGTCTACATT+AGG 0.377042 4:+57280754 None:intergenic
CAATAATGGAGACACAAATA+TGG 0.378026 4:-57280853 MsaT020695.1:CDS
CAAGACTTGCAGAAATATCT+TGG 0.387546 4:+57281425 None:intergenic
AGGAATGTTCCTGTTGGTGG+GGG 0.412807 4:-57281371 MsaT020695.1:CDS
TTCTTCTAGTGGACAACAAC+AGG 0.413714 4:-57282309 MsaT020695.1:intron
TGTGAGATCATTGGAATCAT+AGG 0.425382 4:+57281247 None:intergenic
GGATTTGGGTTGGTTTGAAA+TGG 0.429582 4:+57281293 None:intergenic
TACCACATGAGGTGTTGGTT+TGG 0.441239 4:+57281153 None:intergenic
AGCATTAGCTAGGCTTCAAA+AGG 0.442460 4:-57281222 MsaT020695.1:CDS
GCAAATGTCTAACCAGTCAT+TGG 0.467897 4:-57281582 MsaT020695.1:CDS
TGTGGTATTCTTAACAATCA+TGG 0.480447 4:-57281137 MsaT020695.1:intron
AAGAAGTGTTTGTATGGGGA+TGG 0.488483 4:-57282333 None:intergenic
AGGCTTCAAAAGGGTCAACT+TGG 0.489311 4:-57281212 MsaT020695.1:CDS
ACAAGTGAGTGGATTTGGGT+TGG 0.491105 4:+57281283 None:intergenic
TAAGGAATGTTCCTGTTGGT+GGG 0.496140 4:-57281373 MsaT020695.1:CDS
AATGGTCTCACTTCATCTTG+GGG 0.497543 4:-57280798 MsaT020695.1:CDS
TGTAGGAGATATTGGACTAA+AGG 0.499003 4:-57281404 MsaT020695.1:CDS
GTGTTGTTTGAGTCACATCT+TGG 0.500769 4:+57281485 None:intergenic
ATGGATCATTTAGGAAGAGC+TGG 0.502060 4:-57280825 MsaT020695.1:CDS
TAATGCTAGTGTGAGATCAT+TGG 0.503293 4:+57281238 None:intergenic
TTGGAATCATAGGACATTGA+AGG 0.508739 4:+57281257 None:intergenic
TTAGGAAGAGCTGGTTGGAA+TGG 0.509850 4:-57280816 MsaT020695.1:CDS
CTAAGGAATGTTCCTGTTGG+TGG 0.517333 4:-57281374 MsaT020695.1:CDS
AAGAATACCACATGAGGTGT+TGG 0.521494 4:+57281148 None:intergenic
ATGGGGATGGATTCTTCTAG+TGG 0.553754 4:-57282320 MsaT020695.1:CDS
TTTAGAGCTTCTGGTTGAGG+AGG 0.557206 4:+57281512 None:intergenic
AAGGAATGTTCCTGTTGGTG+GGG 0.558824 4:-57281372 MsaT020695.1:CDS
TTAACATGCTCTCCAATGAC+TGG 0.564124 4:+57281570 None:intergenic
GGACTAAAGGTGGTACACTA+AGG 0.568077 4:-57281391 MsaT020695.1:CDS
ATCATTTAGGAAGAGCTGGT+TGG 0.573027 4:-57280821 MsaT020695.1:CDS
CGAGACGGTGTCTACATTAG+GGG 0.579103 4:+57280756 None:intergenic
TGGAATCATAGGACATTGAA+GGG 0.583945 4:+57281258 None:intergenic
GGAATGTTCCTGTTGGTGGG+GGG 0.588003 4:-57281370 MsaT020695.1:CDS
ATCTCACACTAGCATTAGCT+AGG 0.588061 4:-57281232 MsaT020695.1:CDS
CATCATCAGCTTCATCAAAG+AGG 0.595811 4:-57281328 MsaT020695.1:CDS
AACCAAACCAACACCTCATG+TGG 0.607094 4:-57281155 MsaT020695.1:CDS
ATGGTCTCACTTCATCTTGG+GGG 0.616298 4:-57280797 MsaT020695.1:CDS
TCTCCATTATTGAACTGCCA+TGG 0.629912 4:+57280864 None:intergenic
GCATTAGCTAGGCTTCAAAA+GGG 0.630153 4:-57281221 MsaT020695.1:CDS
GAGACGGTGTCTACATTAGG+GGG 0.640287 4:+57280757 None:intergenic
ATTGTTAAGAATACCACATG+AGG 0.643870 4:+57281142 None:intergenic
AGGAGATATTGGACTAAAGG+TGG 0.646558 4:-57281401 MsaT020695.1:CDS
ACACTAAGGAATGTTCCTGT+TGG 0.660187 4:-57281377 MsaT020695.1:CDS
ATTGAAGGGAAACAAGTGAG+TGG 0.662440 4:+57281272 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 57280767 57282342 57280767 ID=MsaG020695
Chr4 mRNA 57280767 57282342 57280767 ID=MsaT020695.1;Parent=MsaG020695
Chr4 exon 57280767 57280891 57280767 ID=MsaT020695.1.exon3;Parent=MsaT020695.1
Chr4 CDS 57280767 57280891 57280767 ID=cds.MsaT020695.1;Parent=MsaT020695.1
Chr4 exon 57281138 57281603 57281138 ID=MsaT020695.1.exon2;Parent=MsaT020695.1
Chr4 CDS 57281138 57281603 57281138 ID=cds.MsaT020695.1;Parent=MsaT020695.1
Chr4 exon 57282310 57282342 57282310 ID=MsaT020695.1.exon1;Parent=MsaT020695.1
Chr4 CDS 57282310 57282342 57282310 ID=cds.MsaT020695.1;Parent=MsaT020695.1
Gene Sequence

>MsaT020695.1

ATGGGGATGGATTCTTCTAGTGGACAACAACAGCAAATGTCTAACCAGTCATTGGAGAGCATGTTAACATGTTCAAAAGCAGAACAAGACAAAAAGCCAAAACCTCCTCAACCAGAAGCTCTAAAATGTCCAAGATGTGACTCAAACAACACCAAATTTTGTTACTACAACAATTACAGTCTTTCTCAACCAAGATATTTCTGCAAGTCTTGTAGGAGATATTGGACTAAAGGTGGTACACTAAGGAATGTTCCTGTTGGTGGGGGGTGTAGAAAGAACAAAAGATCATCATCAGCTTCATCAAAGAGGTCTCAAGATCAACCATTTCAAACCAACCCAAATCCACTCACTTGTTTCCCTTCAATGTCCTATGATTCCAATGATCTCACACTAGCATTAGCTAGGCTTCAAAAGGGTCAACTTGGATTTGATCATGAACATGATTTTTCCATTTTAGGAAACCAAACCAACACCTCATGTGGTATTCTTAACAATCATGGTTTTCCATGGCAGTTCAATAATGGAGACACAAATATGGCTGAAATGGATCATTTAGGAAGAGCTGGTTGGAATGGTCTCACTTCATCTTGGGGGCATGGCCTTCTCAATAGCCCCCTAATGTAG

Protein sequence

>MsaT020695.1

MGMDSSSGQQQQMSNQSLESMLTCSKAEQDKKPKPPQPEALKCPRCDSNNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRSSSASSKRSQDQPFQTNPNPLTCFPSMSYDSNDLTLALARLQKGQLGFDHEHDFSILGNQTNTSCGILNNHGFPWQFNNGDTNMAEMDHLGRAGWNGLTSSWGHGLLNSPLM*