Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020794 | KEH30134.1 | 79.487 | 234 | 5 | 4 | 25 | 216 | 25 | 257 | 4.73e-123 | 359 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020794 | sp|Q6X7K1|WOX2_ARATH | 28.740 | 254 | 104 | 10 | 17 | 208 | 6 | 244 | 7.70e-16 | 77.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020794 | tr|A0A072UK53|A0A072UK53_MEDTR | 79.487 | 234 | 5 | 4 | 25 | 216 | 25 | 257 | 2.26e-123 | 359 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG020794 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020794 | MtrunA17_Chr4g0031341 | 79.487 | 234 | 5 | 4 | 25 | 216 | 25 | 257 | 4.35e-127 | 359 |
| MsaG020794 | MtrunA17_Chr8g0392991 | 57.692 | 52 | 19 | 1 | 12 | 60 | 80 | 131 | 2.93e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG020794 | AT5G59340.1 | 28.740 | 254 | 104 | 10 | 17 | 208 | 6 | 244 | 7.82e-17 | 77.0 |
Find 42 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATAGGTGAAGGATAAA+TGG | 0.223363 | 4:-58659970 | None:intergenic |
| AATCAAGATCATGGTTATAA+TGG | 0.248654 | 4:+58661221 | MsaT020794.1:CDS |
| TTCTGCAGGACAAGGTTCTT+AGG | 0.334683 | 4:+58661274 | MsaT020794.1:CDS |
| CTGTAACCAAAGTCATAGTT+AGG | 0.337773 | 4:-58661053 | None:intergenic |
| AGTTCCACTGATACACATTC+TGG | 0.341293 | 4:+58661176 | MsaT020794.1:CDS |
| AACATACCATTTGGACAAAT+AGG | 0.347632 | 4:-58659984 | None:intergenic |
| ACTGCATCCAACTGGCATAT+TGG | 0.347683 | 4:+58661103 | MsaT020794.1:CDS |
| ACTGCAGGTAGAAGCACTTT+TGG | 0.360138 | 4:-58660930 | None:intergenic |
| TAGCTTATTTCATGACTTTG+TGG | 0.366655 | 4:-58660894 | None:intergenic |
| CCTTCACCTATTTGTCCAAA+TGG | 0.376093 | 4:+58659978 | MsaT020794.1:CDS |
| ATCAAGATCATGGTTATAAT+GGG | 0.392817 | 4:+58661222 | MsaT020794.1:CDS |
| ATGAATGAGGAAGGTTCAAA+TGG | 0.394930 | 4:+58659690 | MsaT020794.1:CDS |
| TGTGGTTGTGGTACGCAATA+TGG | 0.424892 | 4:-58660876 | None:intergenic |
| ATTTCATGACTTTGTGGTTG+TGG | 0.425330 | 4:-58660888 | None:intergenic |
| CAAATAGGTGAAGGATAAAT+GGG | 0.425469 | 4:-58659969 | None:intergenic |
| TCTTGTATTTCTTCAGCACT+TGG | 0.437582 | 4:-58659804 | None:intergenic |
| TTGTTTCCACTGCATCCAAC+TGG | 0.438638 | 4:+58661095 | MsaT020794.1:CDS |
| GCTGAGAAAGTTATGTCAAA+TGG | 0.451730 | 4:+58660972 | MsaT020794.1:CDS |
| AGAATCCCTAACTATGACTT+TGG | 0.454278 | 4:+58661047 | MsaT020794.1:CDS |
| TTCCCTTCCAATATGCCAGT+TGG | 0.473137 | 4:-58661110 | None:intergenic |
| TTGGAGAATTTGTATAAGCA+AGG | 0.498360 | 4:+58659771 | MsaT020794.1:CDS |
| AAAAGAACAAATTAGTATGT+TGG | 0.499598 | 4:+58659752 | MsaT020794.1:CDS |
| CATGATATCAATCAAGATCA+TGG | 0.502262 | 4:+58661212 | MsaT020794.1:CDS |
| CATCCAACTGGCATATTGGA+AGG | 0.509666 | 4:+58661107 | MsaT020794.1:CDS |
| TGAATGAGGAAGGTTCAAAT+GGG | 0.520103 | 4:+58659691 | MsaT020794.1:CDS |
| TTACAGTAATCAAGAAACAT+TGG | 0.520782 | 4:+58661070 | MsaT020794.1:CDS |
| AATAATGATATGAATGAGGA+AGG | 0.537613 | 4:+58659681 | MsaT020794.1:CDS |
| GGTGAAGGATAAATGGGTTG+AGG | 0.539516 | 4:-58659963 | None:intergenic |
| TGTAACCAAAGTCATAGTTA+GGG | 0.542299 | 4:-58661052 | None:intergenic |
| TTTAATAATTCTGCAGGACA+AGG | 0.556573 | 4:+58661266 | MsaT020794.1:CDS |
| TTCCTTCTAAAACCTACTGC+AGG | 0.572590 | 4:-58660945 | None:intergenic |
| CTGAAACAATAGAAGACACC+TGG | 0.584011 | 4:-58661142 | None:intergenic |
| AAAGTGCTTCTACCTGCAGT+AGG | 0.586289 | 4:+58660933 | MsaT020794.1:CDS |
| AATATGCCAGTTGGATGCAG+TGG | 0.586423 | 4:-58661101 | None:intergenic |
| GAATAATAATGATATGAATG+AGG | 0.589218 | 4:+58659677 | MsaT020794.1:CDS |
| AGAACCAGAATGTGTATCAG+TGG | 0.599607 | 4:-58661180 | None:intergenic |
| AAATATCTGCAATAGTCCAA+TGG | 0.604277 | 4:+58661001 | MsaT020794.1:CDS |
| CCATTTGGACAAATAGGTGA+AGG | 0.604508 | 4:-58659978 | None:intergenic |
| AAATACAAGAAATCACTGCT+AGG | 0.611419 | 4:+58659817 | MsaT020794.1:CDS |
| ATCCAACTGGCATATTGGAA+GGG | 0.612491 | 4:+58661108 | MsaT020794.1:CDS |
| GGAAGGGAAAACAACTGACC+AGG | 0.627194 | 4:+58661124 | MsaT020794.1:CDS |
| GTATTTCATCAAGTTCACGA+TGG | 0.702362 | 4:+58659721 | MsaT020794.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 58659666 | 58661295 | 58659666 | ID=MsaG020794 |
| Chr4 | mRNA | 58659666 | 58661295 | 58659666 | ID=MsaT020794.1;Parent=MsaG020794 |
| Chr4 | exon | 58659666 | 58659847 | 58659666 | ID=MsaT020794.1.exon1;Parent=MsaT020794.1 |
| Chr4 | CDS | 58659666 | 58659847 | 58659666 | ID=cds.MsaT020794.1;Parent=MsaT020794.1 |
| Chr4 | exon | 58659962 | 58659999 | 58659962 | ID=MsaT020794.1.exon2;Parent=MsaT020794.1 |
| Chr4 | CDS | 58659962 | 58659999 | 58659962 | ID=cds.MsaT020794.1;Parent=MsaT020794.1 |
| Chr4 | exon | 58660865 | 58661295 | 58660865 | ID=MsaT020794.1.exon3;Parent=MsaT020794.1 |
| Chr4 | CDS | 58660865 | 58661295 | 58660865 | ID=cds.MsaT020794.1;Parent=MsaT020794.1 |
| Gene Sequence |
| Protein sequence |
