AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020797

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020797 MtrunA17_Chr4g0031411 92.674 273 16 2 39 310 65 334 4.07e-168 470
MsaG020797 MtrunA17_Chr2g0328021 40.659 273 113 10 45 310 58 288 2.84e-48 163
MsaG020797 MtrunA17_Chr4g0010151 37.500 272 117 10 46 291 79 323 1.69e-40 144
MsaG020797 MtrunA17_Chr7g0237921 41.897 253 107 8 46 286 73 297 4.17e-40 143
MsaG020797 MtrunA17_Chr7g0223311 83.582 67 10 1 48 113 76 142 3.67e-34 128
MsaG020797 MtrunA17_Chr4g0047001 66.667 81 23 1 50 130 56 132 1.18e-32 124
MsaG020797 MtrunA17_Chr8g0374251 60.674 89 26 1 49 137 68 147 1.28e-31 120
MsaG020797 MtrunA17_Chr8g0367161 69.620 79 20 2 48 126 31 105 2.62e-31 119
MsaG020797 MtrunA17_Chr5g0411951 87.037 54 7 0 46 99 40 93 2.77e-31 119
MsaG020797 MtrunA17_Chr4g0030051 85.185 54 8 0 48 101 40 93 3.39e-30 115
MsaG020797 MtrunA17_Chr2g0326001 81.034 58 11 0 46 103 37 94 3.45e-30 115
MsaG020797 MtrunA17_Chr8g0346551 56.383 94 39 1 39 132 14 105 6.89e-30 114
MsaG020797 MtrunA17_Chr3g0127201 63.415 82 25 2 27 108 6 82 1.62e-29 114
MsaG020797 MtrunA17_Chr3g0117601 59.770 87 31 1 48 134 60 142 1.81e-29 114
MsaG020797 MtrunA17_Chr8g0374111 61.176 85 26 2 49 133 29 106 4.69e-29 111
MsaG020797 MtrunA17_Chr1g0188851 78.571 56 12 0 48 103 42 97 5.55e-29 112
MsaG020797 MtrunA17_Chr4g0061761 82.692 52 9 0 48 99 39 90 1.38e-28 112
MsaG020797 MtrunA17_Chr1g0177251 75.862 58 14 0 51 108 14 71 1.41e-28 111
MsaG020797 MtrunA17_Chr2g0282511 88.000 50 6 0 48 97 44 93 1.81e-28 111
MsaG020797 MtrunA17_Chr3g0126201 75.862 58 14 0 46 103 48 105 2.10e-28 112
MsaG020797 MtrunA17_Chr2g0282441 84.314 51 8 0 50 100 26 76 3.83e-28 111
MsaG020797 MtrunA17_Chr8g0341671 70.492 61 18 0 49 109 20 80 4.07e-28 108
MsaG020797 MtrunA17_Chr2g0308011 84.000 50 8 0 48 97 36 85 3.25e-27 105
MsaG020797 MtrunA17_Chr4g0047761 71.186 59 15 1 49 107 72 128 3.91e-25 102
MsaG020797 MtrunA17_Chr2g0283811 64.286 70 24 1 46 115 44 112 5.59e-25 98.6
MsaG020797 MtrunA17_Chr2g0282051 66.129 62 16 1 48 109 45 101 9.09e-25 100
MsaG020797 MtrunA17_Chr8g0355261 67.857 56 18 0 48 103 117 172 7.54e-24 100
MsaG020797 MtrunA17_Chr6g0453321 71.698 53 15 0 49 101 136 188 2.34e-23 99.8
MsaG020797 MtrunA17_Chr4g0042481 69.811 53 16 0 49 101 122 174 3.93e-23 99.0
MsaG020797 MtrunA17_Chr3g0092111 66.667 60 19 1 49 108 136 194 7.35e-23 98.2
MsaG020797 MtrunA17_Chr6g0460721 64.286 56 20 0 46 101 86 141 1.23e-22 95.9
MsaG020797 MtrunA17_Chr7g0254161 69.091 55 16 1 49 103 101 154 1.73e-22 97.1
MsaG020797 MtrunA17_Chr7g0217581 51.282 78 38 0 49 126 232 309 1.77e-22 97.8
MsaG020797 MtrunA17_Chr6g0460731 62.500 56 21 0 46 101 93 148 1.36e-21 93.6
MsaG020797 MtrunA17_Chr5g0417571 62.963 54 20 0 48 101 137 190 5.49e-21 91.3
MsaG020797 MtrunA17_Chr5g0417601 66.667 57 18 1 49 105 140 195 7.24e-21 91.7
MsaG020797 MtrunA17_Chr5g0417561 64.151 53 19 0 49 101 119 171 1.68e-19 87.8
MsaG020797 MtrunA17_Chr5g0417551 64.151 53 19 0 49 101 127 179 1.75e-19 87.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020797 AT1G07640.1 41.418 268 137 10 49 309 21 275 3.17e-42 147
MsaG020797 AT3G55370.1 43.878 196 74 5 39 225 66 234 9.75e-42 147
MsaG020797 AT3G55370.2 43.878 196 74 5 39 225 66 234 1.08e-41 148
MsaG020797 AT3G55370.3 43.878 196 74 5 39 225 111 279 1.71e-41 147
MsaG020797 AT1G07640.2 41.418 268 137 10 49 309 77 331 2.25e-41 146
MsaG020797 AT1G07640.3 41.418 268 137 10 49 309 85 339 2.33e-41 147
MsaG020797 AT2G28810.2 36.519 293 132 10 31 306 59 314 9.61e-38 137
MsaG020797 AT2G28810.1 36.519 293 132 10 31 306 76 331 1.65e-37 136
MsaG020797 AT2G37590.1 93.103 58 4 0 46 103 86 143 5.52e-35 129
MsaG020797 AT5G02460.1 91.379 58 5 0 46 103 92 149 2.14e-33 127
MsaG020797 AT5G60200.1 70.130 77 19 1 39 115 45 117 4.09e-33 123
MsaG020797 AT1G51700.1 61.176 85 25 1 49 133 33 109 1.15e-31 117
MsaG020797 AT5G60850.1 62.791 86 25 2 48 133 50 128 1.37e-31 120
MsaG020797 AT4G38000.1 67.123 73 24 0 31 103 23 95 4.32e-31 117
MsaG020797 AT5G65590.1 77.419 62 14 0 49 110 41 102 1.40e-30 117
MsaG020797 AT3G21270.1 53.000 100 28 2 49 129 29 128 4.81e-30 113
MsaG020797 AT3G45610.1 52.326 86 39 1 46 129 37 122 1.51e-29 113
MsaG020797 AT2G28510.1 67.568 74 20 1 39 112 38 107 7.76e-29 112
MsaG020797 AT3G52440.1 62.651 83 25 2 51 131 27 105 7.89e-29 111
MsaG020797 AT3G52440.2 62.651 83 25 2 51 131 46 124 1.05e-28 111
MsaG020797 AT1G28310.1 79.661 59 11 1 49 107 27 84 2.04e-28 112
MsaG020797 AT1G28310.2 79.661 59 11 1 49 107 51 108 2.65e-28 112
MsaG020797 AT4G24060.1 50.459 109 54 0 46 154 50 158 5.16e-28 111
MsaG020797 AT1G21340.1 71.642 67 11 2 45 111 36 94 6.28e-28 109
MsaG020797 AT5G66940.1 60.000 80 29 1 39 118 22 98 1.51e-27 107
MsaG020797 AT3G61850.2 60.465 86 26 2 49 130 62 143 2.98e-27 108
MsaG020797 AT3G61850.3 60.465 86 26 2 49 130 62 143 2.98e-27 108
MsaG020797 AT5G62940.1 84.000 50 8 0 50 99 74 123 3.72e-27 109
MsaG020797 AT3G61850.1 60.465 86 26 2 49 130 74 155 3.99e-27 108
MsaG020797 AT3G61850.5 60.465 86 26 2 49 130 62 143 4.34e-27 108
MsaG020797 AT3G50410.1 70.690 58 17 0 49 106 30 87 4.77e-27 106
MsaG020797 AT3G61850.4 60.465 86 26 2 49 130 74 155 5.07e-27 108
MsaG020797 AT4G21050.1 74.074 54 14 0 47 100 22 75 2.44e-26 103
MsaG020797 AT4G00940.1 63.636 66 24 0 38 103 57 122 4.07e-26 105
MsaG020797 AT4G00940.3 63.636 66 24 0 38 103 57 122 4.07e-26 105
MsaG020797 AT4G00940.2 63.636 66 24 0 38 103 57 122 4.07e-26 105
MsaG020797 AT2G46590.1 85.417 48 7 0 49 96 68 115 3.22e-25 103
MsaG020797 AT1G64620.1 82.000 50 9 0 48 97 48 97 3.36e-25 103
MsaG020797 AT2G46590.2 85.417 48 7 0 49 96 80 127 3.48e-25 103
MsaG020797 AT4G21040.1 56.250 80 30 1 51 130 27 101 1.74e-23 96.7
MsaG020797 AT1G47655.1 75.510 49 12 0 49 97 29 77 1.93e-23 95.9
MsaG020797 AT2G34140.1 72.727 55 14 1 49 103 58 111 3.52e-23 94.4
MsaG020797 AT1G29160.1 69.492 59 17 1 49 107 62 119 3.79e-23 94.4
MsaG020797 AT3G47500.1 69.811 53 16 0 49 101 110 162 1.18e-22 97.8
MsaG020797 AT5G62430.1 66.667 57 18 1 49 105 54 109 2.27e-22 95.1
MsaG020797 AT5G39660.3 69.811 53 16 0 49 101 138 190 3.78e-22 96.7
MsaG020797 AT5G39660.1 69.811 53 16 0 49 101 138 190 3.78e-22 96.7
MsaG020797 AT5G39660.2 69.811 53 16 0 49 101 138 190 3.78e-22 96.7
MsaG020797 AT4G21080.1 52.381 84 30 2 51 129 27 105 4.30e-22 93.6
MsaG020797 AT1G69570.1 66.038 53 18 0 49 101 132 184 7.51e-22 95.1
MsaG020797 AT1G26790.1 66.038 53 18 0 49 101 111 163 8.38e-22 94.7
MsaG020797 AT4G21030.1 55.357 56 25 0 47 102 19 74 4.87e-15 72.8

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAGACATGTAGGCGTTAT+TGG 0.207030 4:+58711051 MsaT020797.1:CDS
GTTGTTGTTGATGTTGTTCT+TGG 0.217968 4:-58711343 None:intergenic
TCAAGAGCCACCGGTTAAAA+TGG 0.246428 4:+58711616 MsaT020797.1:CDS
TCTTGAGAGTTTGTTGAATT+AGG 0.247926 4:-58711161 None:intergenic
ACCAACAACAATACTATTCT+TGG 0.250684 4:+58711696 MsaT020797.1:CDS
GTTTCTGCTTCTGCTGTTGC+TGG 0.257390 4:+58711428 MsaT020797.1:CDS
GATCATATGGGGTTTCAAAT+TGG 0.271163 4:+58711374 MsaT020797.1:CDS
TATTCTTGGAATGATTTATC+AGG 0.288988 4:+58711710 MsaT020797.1:CDS
TACTGGTGGCGGTGGTACTA+TGG 0.292815 4:+58710880 MsaT020797.1:CDS
CCTTCTCATCATGAACTAAT+TGG 0.327970 4:+58711212 MsaT020797.1:CDS
AACATTGAAGCTGCAGCATC+TGG 0.352153 4:+58711557 MsaT020797.1:CDS
AATTCCTAACCTACCCTTCA+TGG 0.365060 4:+58711253 MsaT020797.1:CDS
ACAACAACAACAAGATCATA+TGG 0.370266 4:+58711361 MsaT020797.1:CDS
TCTGCTGTTGCTGGAGGTGT+TGG 0.398368 4:+58711437 MsaT020797.1:CDS
TTTCAAATTGGTATTGGTAA+TGG 0.399190 4:+58711386 MsaT020797.1:CDS
TCCAAGAATAGTATTGTTGT+TGG 0.406762 4:-58711697 None:intergenic
ATGGGGTTTCAAATTGGTAT+TGG 0.408013 4:+58711380 MsaT020797.1:CDS
CTACGACACCCTCCACCAAC+TGG 0.417773 4:-58711101 None:intergenic
TGAGAAGGGATTGCACTTGA+TGG 0.431346 4:-58711197 None:intergenic
GCTCGATCAGCCATGGAGCC+TGG 0.444337 4:-58710914 None:intergenic
CCTCAAGTGGGAGGTAGTAC+TGG 0.448394 4:+58710863 MsaT020797.1:CDS
GATTCAAAAGTATTCAAGAA+AGG 0.460724 4:-58711491 None:intergenic
TTCTTGATAAATTTAAAGCT+CGG 0.472306 4:-58711646 None:intergenic
CTTAGAAATGTTCCAGTTGG+TGG 0.472325 4:+58711089 MsaT020797.1:CDS
CAACAACAACAAGATCATAT+GGG 0.473282 4:+58711362 MsaT020797.1:CDS
AATTTATCAAGAACATCTCT+TGG 0.474556 4:+58711656 MsaT020797.1:CDS
CTCGATCAGACCAGGCTCCA+TGG 0.475078 4:+58710904 MsaT020797.1:CDS
GAGATATAGCTTCAAACTCT+AGG 0.480948 4:+58711588 MsaT020797.1:CDS
CCTCCCACTTGAGGTGGTAA+AGG 0.483237 4:-58710854 None:intergenic
GGTAGGTTAGGAATTGTTTG+TGG 0.484498 4:-58711245 None:intergenic
GAGGCCATGAAGGGTAGGTT+AGG 0.490552 4:-58711257 None:intergenic
AGAAATGTTCCAGTTGGTGG+AGG 0.497986 4:+58711092 MsaT020797.1:CDS
CCAATTAGTTCATGATGAGA+AGG 0.516486 4:-58711212 None:intergenic
GCTGCAGCATCTGGATTCGT+TGG 0.518066 4:+58711566 MsaT020797.1:CDS
GAAGTAGCAAAATTTAGTGT+TGG 0.523027 4:-58710994 None:intergenic
AGAAGTGACGAGGTTGAGTA+AGG 0.524057 4:-58711028 None:intergenic
ACTGGTGGCGGTGGTACTAT+GGG 0.530847 4:+58710881 MsaT020797.1:CDS
GGAAACGTTAACATGGGACT+TGG 0.531022 4:+58711308 MsaT020797.1:CDS
GAGAAGGGATTGCACTTGAT+GGG 0.532889 4:-58711196 None:intergenic
CAACGAGGACACTTTAGCGC+TGG 0.540252 4:-58710963 None:intergenic
ACACTTTAGCGCTGGTTCAG+TGG 0.540912 4:-58710955 None:intergenic
TCTGCTTCTGCTGTTGCTGG+AGG 0.548891 4:+58711431 MsaT020797.1:CDS
AGATATAGCTTCAAACTCTA+GGG 0.556521 4:+58711589 MsaT020797.1:CDS
GGAGGTAGTACTGGTGGCGG+TGG 0.560481 4:+58710872 MsaT020797.1:CDS
ATTCTGCATCGAGGCCATGA+AGG 0.561082 4:-58711267 None:intergenic
AATCTGAGTCGTTATGTCGT+TGG 0.570962 4:+58711287 MsaT020797.1:CDS
CTAACGAGCTCGATCAGCCA+TGG 0.573212 4:-58710921 None:intergenic
CAATTAGTTCATGATGAGAA+GGG 0.573984 4:-58711211 None:intergenic
TGTCGTTGGAAACGTTAACA+TGG 0.575317 4:+58711301 MsaT020797.1:CDS
TCCACCTTTACCACCTCAAG+TGG 0.577272 4:+58710850 MsaT020797.1:CDS
ACTATGGGCTCGATCAGACC+AGG 0.580839 4:+58710896 MsaT020797.1:CDS
TTCTGCATCGAGGCCATGAA+GGG 0.581643 4:-58711266 None:intergenic
CCCACTTGAGGTGGTAAAGG+TGG 0.581750 4:-58710851 None:intergenic
GAAATGTTCCAGTTGGTGGA+GGG 0.594932 4:+58711093 MsaT020797.1:CDS
CTTTAGCGCTGGTTCAGTGG+TGG 0.596893 4:-58710952 None:intergenic
GCACTTAGAAATGTTCCAGT+TGG 0.600640 4:+58711086 MsaT020797.1:CDS
AGAAGGGATTGCACTTGATG+GGG 0.604506 4:-58711195 None:intergenic
GTAGGCGTTATTGGACAAGA+GGG 0.605161 4:+58711060 MsaT020797.1:CDS
TAGGGCTAATCAAGAGCCAC+CGG 0.605644 4:+58711607 MsaT020797.1:CDS
GTCGTTGGAAACGTTAACAT+GGG 0.609381 4:+58711302 MsaT020797.1:CDS
GTCACTTCTGCAAGACATGT+AGG 0.611130 4:+58711042 MsaT020797.1:CDS
TGTAGGCGTTATTGGACAAG+AGG 0.616737 4:+58711059 MsaT020797.1:CDS
GCATCGAGGCCATGAAGGGT+AGG 0.623817 4:-58711262 None:intergenic
AATTGCTGCAAATTACGCCA+TGG 0.629104 4:-58711467 None:intergenic
CATGTCTTGCAGAAGTGACG+AGG 0.635723 4:-58711038 None:intergenic
GTGGGAGGTAGTACTGGTGG+CGG 0.638124 4:+58710869 MsaT020797.1:CDS
AACAACAACAAGATCATATG+GGG 0.646211 4:+58711363 MsaT020797.1:CDS
CCACCTTTACCACCTCAAGT+GGG 0.662217 4:+58710851 MsaT020797.1:CDS
CAAGTGGGAGGTAGTACTGG+TGG 0.664836 4:+58710866 MsaT020797.1:CDS
GTACTACCTCCCACTTGAGG+TGG 0.666584 4:-58710860 None:intergenic
TAGGCGTTATTGGACAAGAG+GGG 0.675252 4:+58711061 MsaT020797.1:CDS
AGGCGTTATTGGACAAGAGG+GGG 0.675862 4:+58711062 MsaT020797.1:CDS
CCAGTACTACCTCCCACTTG+AGG 0.681370 4:-58710863 None:intergenic
CCTTTACCACCTCAAGTGGG+AGG 0.700568 4:+58710854 MsaT020797.1:CDS
GAGGTGTTGGAGTTGAACCA+TGG 0.701694 4:+58711450 MsaT020797.1:CDS
GTGTTGGTTGAATCGCAACG+AGG 0.733913 4:-58710978 None:intergenic
ACGACTCAGATTCTGCATCG+AGG 0.741695 4:-58711276 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 58710825 58711757 58710825 ID=MsaG020797
Chr4 mRNA 58710825 58711757 58710825 ID=MsaT020797.1;Parent=MsaG020797
Chr4 exon 58710825 58711757 58710825 ID=MsaT020797.1.exon1;Parent=MsaT020797.1
Chr4 CDS 58710825 58711757 58710825 ID=cds.MsaT020797.1;Parent=MsaT020797.1
Gene Sequence

>MsaT020797.1

ATGGAAGCAATAACACTCAACTTCTTCCACCTTTACCACCTCAAGTGGGAGGTAGTACTGGTGGCGGTGGTACTATGGGCTCGATCAGACCAGGCTCCATGGCTGATCGAGCTCGTTAGCAAAACTACCACCACTGAACCAGCGCTAAAGTGTCCTCGTTGCGATTCAACCAACACTAAATTTTGCTACTTCAACAACTACAGCCTTACTCAACCTCGTCACTTCTGCAAGACATGTAGGCGTTATTGGACAAGAGGGGGTGCACTTAGAAATGTTCCAGTTGGTGGAGGGTGTCGTAGAAACAACAAGAAAAACAAAAGAAGCCGTTCAAAATCTCCTAATTCAACAAACTCTCAAGAAAAACAAACTTCCCCATCAAGTGCAATCCCTTCTCATCATGAACTAATTGGTCATCAGTTACCACAAACAATTCCTAACCTACCCTTCATGGCCTCGATGCAGAATCTGAGTCGTTATGTCGTTGGAAACGTTAACATGGGACTTGGTTTGCGTGAGATCCAAGAACAACATCAACAACAACAACAACAAGATCATATGGGGTTTCAAATTGGTATTGGTAATGGTAACTCAACTTCTGTTTCTGTTTCTGCTTCTGCTGTTGCTGGAGGTGTTGGAGTTGAACCATGGCGTAATTTGCAGCAATTTCCTTTCTTGAATACTTTTGAATCAAACTCTGATGGAAATAATTCATACACTTTTCATGGAGAAAGTAACATTGAAGCTGCAGCATCTGGATTCGTTGGAGATATAGCTTCAAACTCTAGGGCTAATCAAGAGCCACCGGTTAAAATGGAAGAAAACCGAGCTTTAAATTTATCAAGAACATCTCTTGGTGTCTCTGAACATAATAACCAACAACAATACTATTCTTGGAATGATTTATCAGGTTCTTCAACTACTCATCTCTTATAA
Protein sequence

>MsaT020797.1

MEAITLNFFHLYHLKWEVVLVAVVLWARSDQAPWLIELVSKTTTTEPALKCPRCDSTNTKFCYFNNYSLTQPRHFCKTCRRYWTRGGALRNVPVGGGCRRNNKKNKRSRSKSPNSTNSQEKQTSPSSAIPSHHELIGHQLPQTIPNLPFMASMQNLSRYVVGNVNMGLGLREIQEQHQQQQQQDHMGFQIGIGNGNSTSVSVSASAVAGGVGVEPWRNLQQFPFLNTFESNSDGNNSYTFHGESNIEAAASGFVGDIASNSRANQEPPVKMEENRALNLSRTSLGVSEHNNQQQYYSWNDLSGSSTTHLL*