AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020831

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020831 MtrunA17_Chr4g0031741 96.189 656 17 2 1 652 1 652 0.0 1300
MsaG020831 MtrunA17_Chr2g0328411 59.355 620 207 14 65 650 46 654 0.0 704
MsaG020831 MtrunA17_Chr2g0328431 54.440 687 247 13 1 652 1 656 0.0 703
MsaG020831 MtrunA17_Chr2g0328401 58.043 603 203 12 66 631 47 636 0.0 671
MsaG020831 MtrunA17_Chr7g0238891 43.641 747 310 18 8 652 5 742 0.0 566
MsaG020831 MtrunA17_Chr4g0031721 56.092 476 186 9 187 652 224 686 6.19e-180 527
MsaG020831 MtrunA17_Chr4g0031701 46.667 630 269 16 63 652 26 628 1.36e-177 519
MsaG020831 MtrunA17_Chr4g0031711 53.877 503 206 12 163 652 246 735 4.19e-177 521
MsaG020831 MtrunA17_Chr4g0031731 45.873 630 274 15 63 652 26 628 1.67e-173 508
MsaG020831 MtrunA17_Chr2g0328391 54.860 463 184 9 202 652 222 671 6.92e-173 508
MsaG020831 MtrunA17_Chr2g0328341 49.091 440 203 6 218 652 217 640 1.90e-147 442
MsaG020831 MtrunA17_Chr2g0328381 41.205 614 310 15 70 652 50 643 4.62e-147 441
MsaG020831 MtrunA17_Chr2g0328361 45.733 457 200 10 203 652 202 617 1.99e-130 397
MsaG020831 MtrunA17_Chr2g0328371 52.941 255 116 1 351 605 1 251 1.09e-96 297
MsaG020831 MtrunA17_Chr2g0290241 31.630 411 271 6 243 649 20 424 1.52e-64 219
MsaG020831 MtrunA17_Chr5g0447691 33.245 376 246 4 277 649 170 543 3.06e-64 221
MsaG020831 MtrunA17_Chr2g0319091 32.708 373 246 4 280 649 208 578 2.17e-60 212
MsaG020831 MtrunA17_Chr1g0159461 33.333 369 241 4 284 649 225 591 2.57e-60 212
MsaG020831 MtrunA17_Chr3g0125811 31.707 369 247 4 284 649 203 569 1.48e-57 204
MsaG020831 MtrunA17_Chr4g0076091 32.626 377 246 6 279 650 170 543 7.35e-57 201
MsaG020831 MtrunA17_Chr4g0057891 32.041 387 232 9 281 649 146 519 1.95e-56 199
MsaG020831 MtrunA17_Chr3g0101141 31.510 384 254 7 271 649 162 541 4.82e-56 199
MsaG020831 MtrunA17_Chr5g0445331 30.769 377 252 5 280 649 157 531 2.35e-55 197
MsaG020831 MtrunA17_Chr7g0224271 31.202 391 236 6 279 649 295 672 4.65e-55 199
MsaG020831 MtrunA17_Chr8g0376381 32.615 371 225 9 285 649 170 521 4.84e-51 185
MsaG020831 MtrunA17_Chr7g0245601 31.436 369 233 8 287 651 441 793 1.28e-48 182
MsaG020831 MtrunA17_Chr8g0357601 30.491 387 247 7 279 652 177 554 6.93e-48 177
MsaG020831 MtrunA17_Chr3g0110971 30.711 394 233 10 277 649 212 586 1.10e-47 177
MsaG020831 MtrunA17_Chr2g0306641 31.877 389 229 13 277 649 118 486 5.02e-46 170
MsaG020831 MtrunA17_Chr4g0038851 27.610 431 243 8 281 649 45 468 7.64e-46 169
MsaG020831 MtrunA17_Chr3g0085681 27.990 418 240 8 285 649 26 435 1.09e-41 157
MsaG020831 MtrunA17_Chr2g0294461 29.457 387 250 9 279 649 205 584 3.19e-40 155
MsaG020831 MtrunA17_Chr4g0057291 25.455 385 265 8 280 651 288 663 4.12e-40 156
MsaG020831 MtrunA17_Chr1g0200401 29.851 402 239 12 276 649 72 458 6.09e-40 152
MsaG020831 MtrunA17_Chr4g0038761 30.481 374 236 9 287 652 87 444 6.16e-40 152
MsaG020831 MtrunA17_Chr5g0401131 28.010 382 257 9 284 651 114 491 2.32e-36 142
MsaG020831 MtrunA17_Chr4g0039661 27.807 374 248 9 285 649 194 554 7.19e-36 142
MsaG020831 MtrunA17_Chr2g0294471 26.753 385 260 9 279 649 199 575 3.49e-35 140
MsaG020831 MtrunA17_Chr1g0183641 27.857 420 263 15 243 650 86 477 3.57e-34 136
MsaG020831 MtrunA17_Chr7g0270031 27.273 385 251 10 279 649 184 553 3.04e-33 134
MsaG020831 MtrunA17_Chr7g0236851 29.655 290 190 6 366 649 375 656 1.58e-31 130
MsaG020831 MtrunA17_Chr3g0114841 28.193 415 238 18 270 650 112 500 2.11e-30 125
MsaG020831 MtrunA17_Chr1g0194621 24.812 399 272 11 268 649 130 517 6.84e-30 124
MsaG020831 MtrunA17_Chr5g0396491 30.645 248 153 5 252 492 30 265 1.64e-28 119
MsaG020831 MtrunA17_Chr5g0396491 30.000 150 104 1 500 649 329 477 7.12e-17 84.0
MsaG020831 MtrunA17_Chr5g0424131 27.033 418 267 15 258 650 95 499 1.79e-28 119
MsaG020831 MtrunA17_Chr6g0468561 26.385 379 252 10 285 649 296 661 1.14e-27 118
MsaG020831 MtrunA17_Chr8g0383981 27.229 415 267 16 252 652 105 498 9.73e-27 114
MsaG020831 MtrunA17_Chr2g0329591 26.923 390 252 11 284 651 74 452 3.82e-26 112
MsaG020831 MtrunA17_Chr4g0053901 25.180 417 259 11 284 652 59 470 1.42e-24 107
MsaG020831 MtrunA17_Chr5g0401421 24.402 418 263 13 284 652 35 448 1.51e-24 107
MsaG020831 MtrunA17_Chr3g0085361 25.852 352 203 7 347 649 7 349 1.98e-24 105
MsaG020831 MtrunA17_Chr7g0230731 24.138 406 267 9 261 649 346 727 2.84e-19 92.4
MsaG020831 MtrunA17_Chr7g0230771 24.051 395 281 8 261 649 349 730 4.89e-19 91.7
MsaG020831 MtrunA17_Chr4g0012071 26.633 199 138 3 261 457 175 367 2.92e-16 82.4
MsaG020831 MtrunA17_Chr2g0323111 27.778 180 126 4 474 652 132 308 2.58e-13 71.6
MsaG020831 MtrunA17_Chr8g0344101 22.799 443 284 17 245 649 177 599 1.75e-12 70.5
MsaG020831 MtrunA17_Chr8g0373561 28.571 147 98 4 282 421 177 323 7.36e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020831 AT2G29060.2 44.131 673 320 19 11 651 8 656 2.53e-179 525
MsaG020831 AT2G29060.1 43.571 700 322 20 11 651 8 693 3.46e-177 520
MsaG020831 AT1G07530.1 55.752 452 191 6 208 652 318 767 1.49e-176 521
MsaG020831 AT5G59450.1 44.640 625 273 15 39 652 38 600 1.49e-172 505
MsaG020831 AT3G46600.1 46.755 601 248 10 59 652 46 581 1.11e-171 502
MsaG020831 AT2G29065.1 45.528 615 285 14 70 652 34 630 1.02e-165 489
MsaG020831 AT3G46600.3 52.980 453 189 5 205 652 116 549 1.22e-160 473
MsaG020831 AT2G37650.1 51.627 461 206 6 205 651 257 714 1.52e-158 473
MsaG020831 AT2G37650.2 51.627 461 206 6 205 651 245 702 2.49e-158 473
MsaG020831 AT3G46600.2 58.221 371 152 2 284 652 4 373 1.58e-155 453
MsaG020831 AT1G07520.2 51.429 455 196 10 208 652 92 531 3.48e-152 451
MsaG020831 AT1G07520.4 51.429 455 196 10 208 652 92 531 3.48e-152 451
MsaG020831 AT1G07520.1 51.429 455 196 10 208 652 256 695 1.60e-150 452
MsaG020831 AT1G07520.3 51.429 455 196 10 208 652 257 696 1.82e-150 452
MsaG020831 AT1G50600.3 34.234 444 269 9 213 649 98 525 5.80e-67 229
MsaG020831 AT1G50600.1 34.234 444 269 9 213 649 169 596 3.03e-66 228
MsaG020831 AT1G50600.2 34.234 444 269 9 213 649 173 600 5.65e-66 228
MsaG020831 AT5G48150.1 31.072 457 284 8 210 649 47 489 2.02e-62 215
MsaG020831 AT5G48150.4 31.072 457 284 8 210 649 47 489 2.02e-62 215
MsaG020831 AT5G48150.2 31.072 457 284 8 210 649 47 489 2.02e-62 215
MsaG020831 AT5G48150.3 31.072 457 284 8 210 649 47 489 2.02e-62 215
MsaG020831 AT4G17230.1 33.158 380 249 4 275 651 149 526 8.07e-57 201
MsaG020831 AT4G17230.2 33.158 380 249 4 275 651 149 526 8.07e-57 201
MsaG020831 AT2G04890.1 31.903 373 242 5 280 649 49 412 8.25e-57 198
MsaG020831 AT1G21450.1 31.806 371 245 6 284 649 225 592 1.72e-54 196
MsaG020831 AT3G54220.1 32.979 376 221 12 287 651 296 651 1.16e-49 184
MsaG020831 AT3G03450.1 31.620 389 230 12 277 649 176 544 8.66e-48 177
MsaG020831 AT1G66350.1 30.561 481 268 17 198 649 62 505 2.32e-47 175
MsaG020831 AT1G14920.1 31.217 378 237 9 279 649 167 528 9.14e-46 171
MsaG020831 AT2G01570.1 31.771 384 227 12 279 649 219 580 2.55e-45 170
MsaG020831 AT5G17490.1 29.974 387 233 11 277 649 153 515 8.21e-45 168
MsaG020831 AT5G66770.1 31.608 367 239 9 288 649 224 583 1.35e-43 166
MsaG020831 AT3G50650.1 30.618 356 232 7 302 649 193 541 2.00e-41 159
MsaG020831 AT4G37650.1 27.456 397 249 11 283 651 145 530 3.60e-39 152
MsaG020831 AT5G41920.1 27.083 384 251 9 276 649 35 399 6.57e-35 137
MsaG020831 AT5G52510.1 27.632 380 247 10 287 649 271 639 1.09e-34 140
MsaG020831 AT1G63100.2 27.336 428 278 12 245 650 238 654 1.14e-33 137
MsaG020831 AT1G63100.1 27.336 428 278 12 245 650 213 629 1.15e-33 137
MsaG020831 AT1G55580.1 27.179 390 213 12 313 649 73 444 4.87e-31 127
MsaG020831 AT4G08250.1 29.834 362 228 14 303 650 128 477 7.39e-31 127
MsaG020831 AT3G49950.1 28.212 397 239 11 284 651 30 409 1.74e-30 124
MsaG020831 AT1G50420.1 30.531 226 142 4 281 497 54 273 4.20e-29 121
MsaG020831 AT1G50420.1 30.667 150 103 1 500 649 330 478 9.53e-17 84.0
MsaG020831 AT3G13840.1 25.882 425 258 19 251 649 116 509 4.33e-20 94.7
MsaG020831 AT4G00150.1 24.933 373 244 15 285 649 209 553 7.63e-14 75.1
MsaG020831 AT2G45160.1 22.025 395 250 13 285 649 272 638 1.03e-12 71.6
MsaG020831 AT4G36710.1 23.747 379 255 12 285 649 126 484 1.26e-12 70.9
MsaG020831 AT3G60630.1 28.502 207 113 6 352 536 327 520 3.79e-11 66.6

Find 129 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGATTCTGTTCCTTCTTC+TGG 0.154876 4:-59119407 MsaT020831.1:CDS
TTCTGCTAGCTGTTTCTTGC+TGG 0.218999 4:-59117627 MsaT020831.1:CDS
AATATTCAAACCCTATTCTT+AGG 0.247868 4:-59119349 MsaT020831.1:CDS
TTCACTCTATCCACCAGTTC+TGG 0.254183 4:+59117733 None:intergenic
TCGTCGCAATCACAGATTTC+TGG 0.282921 4:-59118813 MsaT020831.1:CDS
GTATATTAGTGATATTCTTA+TGG 0.285545 4:-59119327 MsaT020831.1:CDS
TCAAAGGATGTCGAATTTCT+TGG 0.296573 4:-59118460 MsaT020831.1:CDS
TCTTATGGATGAAGAAGATT+TGG 0.306482 4:-59119312 MsaT020831.1:CDS
TAAGTACTACGGTAGATTTA+TGG 0.318992 4:-59118728 MsaT020831.1:CDS
ATCTTCCTCAGCCAGGATTT+AGG 0.323032 4:-59118287 MsaT020831.1:CDS
CTCAGAAGATTCACCATTTG+AGG 0.340982 4:+59119379 None:intergenic
CCACCAAGGCTTCGTATTAC+AGG 0.346264 4:-59118315 MsaT020831.1:CDS
ACACCATGTATGAAAATGTT+TGG 0.351597 4:+59118021 None:intergenic
ACAATTGCCTTGCTCAGAAA+TGG 0.366886 4:-59118191 MsaT020831.1:CDS
TCACTAATATACCTAAGAAT+AGG 0.370302 4:+59119338 None:intergenic
TCTGTTAACGGATGCATGTT+TGG 0.374306 4:-59119512 MsaT020831.1:CDS
TCTGGTTCAGGAAGATCTAA+TGG 0.377826 4:-59118795 MsaT020831.1:CDS
CTTACAAGCAGTGGCAGGTT+AGG 0.393985 4:-59117792 MsaT020831.1:CDS
GGATGGGAAGTGGGTTCTGC+AGG 0.394105 4:-59117665 MsaT020831.1:CDS
CAATCACAGATTTCTGGTTC+AGG 0.395582 4:-59118807 MsaT020831.1:CDS
TATCAAACAATGATGAGAAA+TGG 0.404180 4:+59117941 None:intergenic
TCTGCTAGCTGTTTCTTGCT+GGG 0.404236 4:-59117626 MsaT020831.1:CDS
TGATGATGTTCTTCTTTGTA+AGG 0.409875 4:-59118874 MsaT020831.1:CDS
ATGATTGAGAAAGGGTTATT+CGG 0.411272 4:-59117874 MsaT020831.1:CDS
GTCTCTTCAACCCTTTCTGC+AGG 0.412650 4:+59118264 None:intergenic
ACTTCCATTGGCTCCAGAAC+TGG 0.415867 4:-59117746 MsaT020831.1:CDS
TTCATACATGGTGTAGTGAA+TGG 0.416386 4:-59118012 MsaT020831.1:CDS
GATGTTCTGGGACCAGCAAC+TGG 0.417189 4:-59119227 MsaT020831.1:CDS
AAATTCGACATCCTTTGAAA+TGG 0.417376 4:+59118465 None:intergenic
CACTAATATACCTAAGAATA+GGG 0.422212 4:+59119339 None:intergenic
ATGATATACTGAAGCAGATT+AGG 0.428145 4:-59118650 MsaT020831.1:CDS
AGTGAAAGAAATGGTGAAGA+AGG 0.428294 4:-59117716 MsaT020831.1:CDS
GTGAAGTACTGCAAGAGGTT+TGG 0.428838 4:-59118228 MsaT020831.1:CDS
TGCCACTGCTTGTAAGTCTC+TGG 0.433473 4:+59117799 None:intergenic
AGCCTCCACATTATTGACTT+TGG 0.433913 4:-59118399 MsaT020831.1:CDS
CGTTGTTGATTGCGATTCTC+CGG 0.436900 4:-59119150 MsaT020831.1:CDS
TCATCAACTACAAAATCTTT+CGG 0.438965 4:+59117688 None:intergenic
GGTTATCTGATGAATGATAA+TGG 0.445118 4:-59118981 MsaT020831.1:CDS
CTAGAACTAGTTCCAGTTGC+TGG 0.450488 4:+59119215 None:intergenic
TCTTCTGGAATAACCTCAAA+TGG 0.456149 4:-59119392 MsaT020831.1:CDS
GCAAGGCAATTGTACTCAAA+TGG 0.459316 4:+59118201 None:intergenic
TGATAAAATTCCTCATGATC+CGG 0.461532 4:-59119105 MsaT020831.1:CDS
TCTATATAATTTGATTAAGC+AGG 0.461754 4:+59117601 None:intergenic
TTTGAGGTTATTCCAGAAGA+AGG 0.463666 4:+59119395 None:intergenic
AAACCTCGCTGATCTCTAAC+CGG 0.468294 4:+59119086 None:intergenic
TTAGCTCATTACTTTGCTGA+TGG 0.473385 4:-59118591 MsaT020831.1:CDS
AGGATTTAGGCCTGCAGAAA+GGG 0.473613 4:-59118274 MsaT020831.1:CDS
CTGATGAATGATAATGGTAT+TGG 0.482313 4:-59118975 MsaT020831.1:CDS
TGGGTTCTGCAGGGTTGGAA+AGG 0.485460 4:-59117655 MsaT020831.1:CDS
GGAAGTGGGTTCTGCAGGGT+TGG 0.485717 4:-59117660 MsaT020831.1:CDS
CGCTGAAGTCCATCGCCACT+TGG 0.487409 4:+59118615 None:intergenic
AAGACATCGCGATCGAAGAA+AGG 0.489585 4:-59118771 MsaT020831.1:CDS
CAATTGCCTTGCTCAGAAAT+GGG 0.493446 4:-59118190 MsaT020831.1:CDS
ATGCTGTGTTGAGGTTGATC+AGG 0.494919 4:-59118053 MsaT020831.1:CDS
GGGTTCTGCAGGGTTGGAAA+GGG 0.502336 4:-59117654 MsaT020831.1:CDS
CAGGATTTAGGCCTGCAGAA+AGG 0.503621 4:-59118275 MsaT020831.1:CDS
ATCGAAGAAAGGTTCCAGTA+AGG 0.504093 4:-59118760 MsaT020831.1:CDS
AAACAATCTTGCAACATACA+AGG 0.512556 4:+59119281 None:intergenic
CTTTCTGCAGGCCTAAATCC+TGG 0.515059 4:+59118276 None:intergenic
TATAGGCTGATGATTGAGAA+AGG 0.518213 4:-59117883 MsaT020831.1:CDS
CCTGTAATACGAAGCCTTGG+TGG 0.518595 4:+59118315 None:intergenic
GAAGAATGTTTCTGATGAAA+CGG 0.519403 4:-59118097 MsaT020831.1:CDS
CATTCTTCGCCAAGTGGCGA+TGG 0.522850 4:-59118624 MsaT020831.1:CDS
TGAGAAAGGGTTATTCGGAA+GGG 0.524037 4:-59117869 MsaT020831.1:CDS
TTACAGCTTCTCCATTTCAA+AGG 0.527701 4:-59118476 MsaT020831.1:CDS
GGAATTGATCTTCCTCAGCC+AGG 0.529102 4:-59118294 MsaT020831.1:CDS
CAACAGAACTATTCTGAAGC+TGG 0.530899 4:-59118436 MsaT020831.1:CDS
AAGGTTCAAACAACTTCCAT+TGG 0.531648 4:-59117758 MsaT020831.1:CDS
AAGGAGCAGAGAGGGTTGAA+AGG 0.532815 4:-59117822 MsaT020831.1:CDS
TCGAAGAAAGGTTCCAGTAA+GGG 0.534571 4:-59118759 MsaT020831.1:CDS
ATGCATCCGTTAACAGAACC+TGG 0.537307 4:+59119518 None:intergenic
GAAGGAACAGAATCACTTGA+AGG 0.539537 4:+59119413 None:intergenic
TCCACCAGTTCTGGAGCCAA+TGG 0.540258 4:+59117742 None:intergenic
AGCTTGTGAAGGAGCAGAGA+GGG 0.541628 4:-59117830 MsaT020831.1:CDS
CAGCGTCTTTCAGAGAGATC+CGG 0.542215 4:-59118342 MsaT020831.1:CDS
GCAGAAAGGGTTGAAGAGAC+AGG 0.545150 4:-59118261 MsaT020831.1:CDS
GATGGGAAGTGGGTTCTGCA+GGG 0.547301 4:-59117664 MsaT020831.1:CDS
TTGAGAAAGGGTTATTCGGA+AGG 0.547490 4:-59117870 MsaT020831.1:CDS
CAGCTTCAGAATAGTTCTGT+TGG 0.547739 4:+59118437 None:intergenic
GATGTTCTTCTTTGTAAGGA+TGG 0.548183 4:-59118870 MsaT020831.1:CDS
AGAGGATTAGGGGAGAAGAG+AGG 0.548379 4:-59119002 MsaT020831.1:CDS
CTTGAGGCGCGATTATCTGC+TGG 0.549108 4:-59118567 MsaT020831.1:CDS
TTACTTTGCTGATGGACTTG+AGG 0.550105 4:-59118583 MsaT020831.1:CDS
GGCCAGAGACTTACAAGCAG+TGG 0.550744 4:-59117801 MsaT020831.1:CDS
TTCAGCTTCCAAACATCACT+CGG 0.553651 4:+59119053 None:intergenic
ACGAAGCCTTGGTGGCCCAC+CGG 0.554118 4:+59118323 None:intergenic
CTGCAACAGACGACGACTGA+AGG 0.556495 4:+59118526 None:intergenic
AGTTGATGAGGATGGGAAGT+GGG 0.558093 4:-59117674 MsaT020831.1:CDS
CGAAGAAAGGTTCCAGTAAG+GGG 0.564272 4:-59118758 MsaT020831.1:CDS
TAGTTGATGAGGATGGGAAG+TGG 0.564567 4:-59117675 MsaT020831.1:CDS
CGGATCTCTCTGAAAGACGC+TGG 0.565013 4:+59118343 None:intergenic
TAGTGTCCCATTTCTGAGCA+AGG 0.568684 4:+59118184 None:intergenic
TGATAGAGAAGAAGTAACAG+TGG 0.569114 4:-59118139 MsaT020831.1:CDS
TTTGGGTTCTAGAGGATTAG+GGG 0.570677 4:-59119012 MsaT020831.1:CDS
AGAGACTTACAAGCAGTGGC+AGG 0.571929 4:-59117797 MsaT020831.1:CDS
ATTCCTGTAATACGAAGCCT+TGG 0.575936 4:+59118312 None:intergenic
CGTCTTTCAGAGAGATCCGG+TGG 0.576548 4:-59118339 MsaT020831.1:CDS
AACCTCAACACAGCATCTCT+AGG 0.577064 4:+59118060 None:intergenic
ATAGGCTGATGATTGAGAAA+GGG 0.583749 4:-59117882 MsaT020831.1:CDS
TCTCAATGTGCACAAGCTGT+TGG 0.587807 4:-59118696 MsaT020831.1:CDS
AGGTTAGGAACAAGAGAGCA+AGG 0.590161 4:-59117777 MsaT020831.1:CDS
TCCATTGGCTCCAGAACTGG+TGG 0.592807 4:-59117743 MsaT020831.1:CDS
GACTTGTGAAGTACTGCAAG+AGG 0.596425 4:-59118233 MsaT020831.1:CDS
AGAGATCCGGTGGGCCACCA+AGG 0.598231 4:-59118329 MsaT020831.1:CDS
CTGTTGGTAGCTATGATCAA+AGG 0.603395 4:-59118680 MsaT020831.1:CDS
CGGAGAATCGCAATCAACAA+CGG 0.604253 4:+59119151 None:intergenic
CGCGTGAAGATGATCAATAT+AGG 0.605875 4:-59117900 MsaT020831.1:CDS
GCAGGCCTAAATCCTGGCTG+AGG 0.614834 4:+59118282 None:intergenic
CTGTGTTGAGGTTGATCAGG+AGG 0.616107 4:-59118050 MsaT020831.1:CDS
GGTGGATAGAGTGAAAGAAA+TGG 0.616135 4:-59117725 MsaT020831.1:CDS
AAATACCAAAGTCAATAATG+TGG 0.616394 4:+59118394 None:intergenic
GTCCTAGAGATGCTGTGTTG+AGG 0.620188 4:-59118062 MsaT020831.1:CDS
GTTTGGAAGCTGAATGAAGA+GGG 0.621160 4:-59119044 MsaT020831.1:CDS
ATGGGACACTATAAGACTTG+AGG 0.623248 4:-59118172 MsaT020831.1:CDS
TACCAAAGTCAATAATGTGG+AGG 0.623436 4:+59118397 None:intergenic
TGATCTCTAACCGGATCATG+AGG 0.623977 4:+59119095 None:intergenic
GAAAGACGCTGGATGAGACA+AGG 0.629384 4:+59118354 None:intergenic
AGGCAGCATTCTTCGCCAAG+TGG 0.643469 4:-59118630 MsaT020831.1:CDS
AGAAGATCTGAGAAGTAACA+AGG 0.644638 4:-59118940 MsaT020831.1:CDS
AAAGGGTTGAAGAGACAGGT+CGG 0.646693 4:-59118257 MsaT020831.1:CDS
TGTTTCTGATGAAACGGTGA+CGG 0.654600 4:-59118091 MsaT020831.1:CDS
GTCTTTCAGAGAGATCCGGT+GGG 0.679118 4:-59118338 MsaT020831.1:CDS
CTATATTGATCATCTTCACG+CGG 0.683616 4:+59117901 None:intergenic
TCCGGAAAATAATGCATACG+AGG 0.702672 4:-59119132 MsaT020831.1:CDS
GTAAATGTGATAGCTTGTGA+AGG 0.707067 4:-59117841 MsaT020831.1:CDS
TAGCTTGTGAAGGAGCAGAG+AGG 0.713278 4:-59117831 MsaT020831.1:CDS
TGTTTGGAAGCTGAATGAAG+AGG 0.744567 4:-59119045 MsaT020831.1:CDS
GACTGAAGGAGTTTATACAT+CGG 0.748275 4:+59118540 None:intergenic
GATCCGGTTAGAGATCAGCG+AGG 0.803140 4:-59119089 MsaT020831.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 59117615 59119573 59117615 ID=MsaG020831
Chr4 mRNA 59117615 59119573 59117615 ID=MsaT020831.1;Parent=MsaG020831
Chr4 exon 59117615 59119573 59117615 ID=MsaT020831.1.exon1;Parent=MsaT020831.1
Chr4 CDS 59117615 59119573 59117615 ID=cds.MsaT020831.1;Parent=MsaT020831.1
Gene Sequence

>MsaT020831.1

ATGTTTTCTACAGAAACAGTCGTAGAAAATTTTCCAGGTTCTGTTAACGGATGCATGTTTGGAAACATACCCGTGTCTGTTTTTTCAAACCAAAACCCAGAAAACGCGTTTAAATTCGAAGATTCAAATTCAATATCACCTTCAAGTGATTCTGTTCCTTCTTCTGGAATAACCTCAAATGGTGAATCTTCTGAGAGTAGTAAATATTCAAACCCTATTCTTAGGTATATTAGTGATATTCTTATGGATGAAGAAGATTTGGAGACAAAACCTTGTATGTTGCAAGATTGTTTGAAGCTTCAAGCTGCTGAAAAATCGTTTTATGATGTTCTGGGACCAGCAACTGGAACTAGTTCTAGTTCTGAGAGTTATAGTAATTGTAGTTATAATACTGATAATTCCGTTGTTGATTGCGATTCTCCGGAAAATAATGCATACGAGGTGTTTGATAAAATTCCTCATGATCCGGTTAGAGATCAGCGAGGTTTTTTCGGTTTTCCGAGTGATGTTTGGAAGCTGAATGAAGAGGGTAGTTTTGTTTTGGGTTCTAGAGGATTAGGGGAGAAGAGAGGTTATCTGATGAATGATAATGGTATTGGTATTTCACATGAAGAAGATCTGAGAAGTAACAAGGTTTCAGCTGTTTATTATGATGATACAGAGTTATCAGAAATGTTTGATGATGTTCTTCTTTGTAAGGATGGTAAAAGTCCGTCGATTTTTTGTGATAATTCTGAGTCGTCGCAATCACAGATTTCTGGTTCAGGAAGATCTAATGGAAAGACATCGCGATCGAAGAAAGGTTCCAGTAAGGGGAAAAAACTAAGTACTACGGTAGATTTATGGACTTTGTTATCTCAATGTGCACAAGCTGTTGGTAGCTATGATCAAAGGAATGCAAATGATATACTGAAGCAGATTAGGCAGCATTCTTCGCCAAGTGGCGATGGACTTCAGCGTTTAGCTCATTACTTTGCTGATGGACTTGAGGCGCGATTATCTGCTGGTACACCGATGTATAAACTCCTTCAGTCGTCGTCTGTTGCAGATATGTTGAGAGCTCATAAAGTTTATATTACAGCTTCTCCATTTCAAAGGATGTCGAATTTCTTGGCCAACAGAACTATTCTGAAGCTGGTAGAAAATAAAAGCAGCCTCCACATTATTGACTTTGGTATTTTCTATGGCTTTCAGTGGCCTTGTCTCATCCAGCGTCTTTCAGAGAGATCCGGTGGGCCACCAAGGCTTCGTATTACAGGAATTGATCTTCCTCAGCCAGGATTTAGGCCTGCAGAAAGGGTTGAAGAGACAGGTCGGCGACTTGTGAAGTACTGCAAGAGGTTTGGAGTTCCATTTGAGTACAATTGCCTTGCTCAGAAATGGGACACTATAAGACTTGAGGATCTCAAGATTGATAGAGAAGAAGTAACAGTGGTTAACTGCTTACACAGATTGAAGAATGTTTCTGATGAAACGGTGACGGCGAATTGTCCTAGAGATGCTGTGTTGAGGTTGATCAGGAGGATTAATCCAAACATTTTCATACATGGTGTAGTGAATGGAACTTATAACGCACCATTTTTTCTTACTAGGTTCAGAGAAGCACTATTCCATTTCTCATCATTGTTTGATATGCTTGAAGCTACTGTGCCGCGTGAAGATGATCAATATAGGCTGATGATTGAGAAAGGGTTATTCGGAAGGGATGCAGTAAATGTGATAGCTTGTGAAGGAGCAGAGAGGGTTGAAAGGCCAGAGACTTACAAGCAGTGGCAGGTTAGGAACAAGAGAGCAAGGTTCAAACAACTTCCATTGGCTCCAGAACTGGTGGATAGAGTGAAAGAAATGGTGAAGAAGGAGTATCCGAAAGATTTTGTAGTTGATGAGGATGGGAAGTGGGTTCTGCAGGGTTGGAAAGGGCGTATTCTGCTAGCTGTTTCTTGCTGGGTTCCTGCTTAA
Protein sequence

>MsaT020831.1

MFSTETVVENFPGSVNGCMFGNIPVSVFSNQNPENAFKFEDSNSISPSSDSVPSSGITSNGESSESSKYSNPILRYISDILMDEEDLETKPCMLQDCLKLQAAEKSFYDVLGPATGTSSSSESYSNCSYNTDNSVVDCDSPENNAYEVFDKIPHDPVRDQRGFFGFPSDVWKLNEEGSFVLGSRGLGEKRGYLMNDNGIGISHEEDLRSNKVSAVYYDDTELSEMFDDVLLCKDGKSPSIFCDNSESSQSQISGSGRSNGKTSRSKKGSSKGKKLSTTVDLWTLLSQCAQAVGSYDQRNANDILKQIRQHSSPSGDGLQRLAHYFADGLEARLSAGTPMYKLLQSSSVADMLRAHKVYITASPFQRMSNFLANRTILKLVENKSSLHIIDFGIFYGFQWPCLIQRLSERSGGPPRLRITGIDLPQPGFRPAERVEETGRRLVKYCKRFGVPFEYNCLAQKWDTIRLEDLKIDREEVTVVNCLHRLKNVSDETVTANCPRDAVLRLIRRINPNIFIHGVVNGTYNAPFFLTRFREALFHFSSLFDMLEATVPREDDQYRLMIEKGLFGRDAVNVIACEGAERVERPETYKQWQVRNKRARFKQLPLAPELVDRVKEMVKKEYPKDFVVDEDGKWVLQGWKGRILLAVSCWVPA*