AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020832

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020832 MtrunA17_Chr4g0031701 93.790 628 39 0 1 628 1 628 0.0 1209
MsaG020832 MtrunA17_Chr4g0031731 92.197 628 49 0 1 628 1 628 0.0 1190
MsaG020832 MtrunA17_Chr4g0031711 71.235 664 141 8 14 628 73 735 0.0 931
MsaG020832 MtrunA17_Chr2g0328391 53.228 697 204 12 2 628 27 671 0.0 693
MsaG020832 MtrunA17_Chr4g0031721 48.584 671 269 14 24 628 26 686 0.0 593
MsaG020832 MtrunA17_Chr7g0238891 43.924 683 297 15 26 628 66 742 0.0 539
MsaG020832 MtrunA17_Chr2g0328431 44.783 623 312 12 27 628 45 656 1.92e-169 498
MsaG020832 MtrunA17_Chr4g0031741 51.767 481 203 7 169 628 180 652 1.31e-168 496
MsaG020832 MtrunA17_Chr2g0328411 45.353 624 303 17 27 626 45 654 1.89e-168 496
MsaG020832 MtrunA17_Chr2g0328341 48.608 467 217 9 174 628 185 640 3.98e-152 453
MsaG020832 MtrunA17_Chr2g0328401 42.764 615 276 16 36 607 55 636 4.79e-152 452
MsaG020832 MtrunA17_Chr2g0328381 41.346 624 307 16 33 628 51 643 1.43e-151 452
MsaG020832 MtrunA17_Chr2g0328361 44.561 478 223 9 161 628 172 617 4.61e-132 400
MsaG020832 MtrunA17_Chr2g0328371 52.157 255 116 3 328 581 2 251 1.81e-92 285
MsaG020832 MtrunA17_Chr2g0319091 34.043 376 243 3 250 625 208 578 9.00e-70 236
MsaG020832 MtrunA17_Chr5g0447691 30.952 420 279 4 212 625 129 543 4.22e-64 220
MsaG020832 MtrunA17_Chr4g0076091 31.579 380 252 6 249 626 170 543 2.14e-62 216
MsaG020832 MtrunA17_Chr3g0101141 32.181 376 250 3 250 625 171 541 3.49e-62 215
MsaG020832 MtrunA17_Chr7g0224271 32.000 400 235 5 245 625 291 672 5.29e-60 212
MsaG020832 MtrunA17_Chr5g0445331 31.842 380 250 7 250 625 157 531 2.40e-58 204
MsaG020832 MtrunA17_Chr1g0159461 29.412 374 259 3 252 625 223 591 2.61e-57 203
MsaG020832 MtrunA17_Chr2g0290241 29.024 379 261 6 249 625 52 424 1.50e-56 197
MsaG020832 MtrunA17_Chr3g0125811 29.412 374 259 3 252 625 201 569 3.29e-56 199
MsaG020832 MtrunA17_Chr4g0057891 30.256 390 241 8 251 625 146 519 1.91e-54 194
MsaG020832 MtrunA17_Chr8g0357601 30.594 438 271 11 205 628 136 554 1.59e-53 192
MsaG020832 MtrunA17_Chr3g0110971 30.900 411 252 8 229 625 194 586 2.26e-53 192
MsaG020832 MtrunA17_Chr2g0306641 30.233 387 248 6 245 625 116 486 3.88e-50 181
MsaG020832 MtrunA17_Chr8g0376381 31.122 392 233 10 243 625 158 521 1.27e-49 181
MsaG020832 MtrunA17_Chr7g0245601 30.270 370 240 7 258 627 442 793 2.11e-49 184
MsaG020832 MtrunA17_Chr5g0396491 26.517 445 259 6 240 625 42 477 2.63e-49 179
MsaG020832 MtrunA17_Chr4g0038851 26.477 457 267 6 229 625 21 468 1.73e-48 176
MsaG020832 MtrunA17_Chr4g0038761 27.493 371 255 5 258 628 88 444 1.11e-41 157
MsaG020832 MtrunA17_Chr4g0057291 27.735 393 260 10 246 627 284 663 1.17e-41 160
MsaG020832 MtrunA17_Chr3g0085681 25.178 421 254 8 255 625 26 435 5.29e-40 152
MsaG020832 MtrunA17_Chr2g0294461 26.667 405 256 8 243 625 199 584 7.14e-37 145
MsaG020832 MtrunA17_Chr1g0200401 29.500 400 239 13 251 625 77 458 4.66e-36 141
MsaG020832 MtrunA17_Chr1g0183641 25.840 387 255 11 246 625 115 476 1.70e-35 140
MsaG020832 MtrunA17_Chr5g0401131 27.083 384 266 8 253 627 113 491 3.90e-35 139
MsaG020832 MtrunA17_Chr4g0039661 26.966 356 242 8 274 625 213 554 1.61e-32 132
MsaG020832 MtrunA17_Chr2g0294471 25.445 393 273 8 243 625 193 575 9.73e-32 130
MsaG020832 MtrunA17_Chr7g0236851 26.899 316 217 6 317 626 350 657 1.57e-31 130
MsaG020832 MtrunA17_Chr7g0270031 26.185 401 253 10 243 625 178 553 2.57e-28 119
MsaG020832 MtrunA17_Chr5g0401421 22.406 424 276 10 251 628 32 448 2.72e-28 118
MsaG020832 MtrunA17_Chr3g0114841 24.272 412 276 11 237 626 103 500 1.74e-27 116
MsaG020832 MtrunA17_Chr5g0424131 25.653 421 266 13 232 626 100 499 3.47e-27 115
MsaG020832 MtrunA17_Chr3g0085361 24.225 355 211 7 320 625 4 349 4.64e-27 112
MsaG020832 MtrunA17_Chr1g0194621 24.876 402 264 12 243 625 135 517 1.51e-26 114
MsaG020832 MtrunA17_Chr4g0053901 23.990 421 271 11 251 628 56 470 2.40e-26 112
MsaG020832 MtrunA17_Chr2g0329591 25.982 331 225 7 226 552 50 364 3.37e-25 108
MsaG020832 MtrunA17_Chr6g0468561 22.636 349 246 7 286 625 328 661 1.83e-22 102
MsaG020832 MtrunA17_Chr8g0383981 25.000 396 246 16 251 628 136 498 7.21e-22 99.4
MsaG020832 MtrunA17_Chr7g0230771 22.520 373 269 7 255 625 376 730 3.16e-17 85.9
MsaG020832 MtrunA17_Chr7g0230731 22.520 373 269 7 255 625 373 727 6.25e-17 84.7
MsaG020832 MtrunA17_Chr8g0344101 25.248 404 243 16 254 625 223 599 1.14e-14 77.4
MsaG020832 MtrunA17_Chr4g0012071 21.932 383 258 9 255 625 197 550 1.77e-14 76.6
MsaG020832 MtrunA17_Chr8g0373561 21.680 369 250 13 270 625 198 540 7.75e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020832 AT1G07530.1 44.993 689 277 15 35 628 86 767 0.0 563
MsaG020832 AT2G29060.2 47.520 625 281 16 29 627 53 656 0.0 551
MsaG020832 AT2G29060.1 45.833 648 295 14 29 627 53 693 0.0 543
MsaG020832 AT2G29065.1 46.505 658 294 16 1 628 1 630 0.0 536
MsaG020832 AT1G07520.3 46.276 631 296 12 36 628 71 696 2.38e-177 520
MsaG020832 AT1G07520.1 46.276 631 296 12 36 628 70 695 2.44e-177 520
MsaG020832 AT1G07520.2 49.718 531 238 9 124 628 4 531 2.66e-175 509
MsaG020832 AT1G07520.4 49.718 531 238 9 124 628 4 531 2.66e-175 509
MsaG020832 AT2G37650.1 41.284 654 318 17 28 627 73 714 1.90e-157 469
MsaG020832 AT2G37650.2 41.131 654 319 17 28 627 61 702 1.97e-157 469
MsaG020832 AT3G46600.3 47.166 441 224 4 190 628 116 549 8.90e-144 429
MsaG020832 AT3G46600.1 47.166 441 224 4 190 628 148 581 1.37e-143 429
MsaG020832 AT5G59450.1 45.140 463 235 7 180 628 143 600 2.29e-141 425
MsaG020832 AT3G46600.2 50.000 378 184 2 251 628 1 373 5.71e-137 405
MsaG020832 AT5G48150.1 31.383 376 254 3 250 625 118 489 1.54e-62 215
MsaG020832 AT5G48150.4 31.383 376 254 3 250 625 118 489 1.54e-62 215
MsaG020832 AT5G48150.2 31.383 376 254 3 250 625 118 489 1.54e-62 215
MsaG020832 AT5G48150.3 31.383 376 254 3 250 625 118 489 1.54e-62 215
MsaG020832 AT1G50600.3 32.181 376 250 3 250 625 155 525 3.41e-61 213
MsaG020832 AT1G50600.1 32.181 376 250 3 250 625 226 596 1.08e-60 213
MsaG020832 AT1G50600.2 32.181 376 250 3 250 625 230 600 1.25e-60 213
MsaG020832 AT2G04890.1 32.181 376 243 4 250 625 49 412 3.67e-60 207
MsaG020832 AT1G21450.1 31.300 377 249 5 252 625 223 592 3.25e-57 203
MsaG020832 AT1G66350.1 32.383 386 234 8 243 625 144 505 3.18e-55 196
MsaG020832 AT4G17230.1 31.398 379 255 3 249 627 153 526 3.22e-53 191
MsaG020832 AT4G17230.2 31.398 379 255 3 249 627 153 526 3.22e-53 191
MsaG020832 AT1G14920.1 31.008 387 244 9 243 625 161 528 1.04e-49 181
MsaG020832 AT3G03450.1 29.016 386 258 7 243 625 172 544 1.33e-49 181
MsaG020832 AT1G50420.1 25.107 466 280 6 217 625 25 478 1.69e-49 179
MsaG020832 AT3G54220.1 29.189 370 247 6 258 627 297 651 5.75e-49 181
MsaG020832 AT2G01570.1 30.233 387 247 9 243 625 213 580 1.85e-48 179
MsaG020832 AT5G17490.1 28.346 381 249 8 249 625 155 515 2.64e-44 166
MsaG020832 AT5G66770.1 27.820 399 266 9 236 625 198 583 9.59e-43 162
MsaG020832 AT5G41920.1 25.728 412 283 7 219 625 6 399 3.14e-40 152
MsaG020832 AT4G37650.1 25.831 391 269 9 253 627 145 530 2.29e-37 146
MsaG020832 AT1G63100.1 31.157 337 216 8 247 579 245 569 1.68e-36 145
MsaG020832 AT1G63100.2 31.157 337 216 8 247 579 270 594 2.05e-36 145
MsaG020832 AT5G52510.1 24.227 388 266 7 252 625 266 639 4.38e-34 138
MsaG020832 AT3G50650.1 27.864 323 214 5 263 579 184 493 1.82e-32 132
MsaG020832 AT3G49950.1 26.904 394 260 11 251 627 27 409 1.17e-27 116
MsaG020832 AT1G55580.1 25.178 421 252 11 251 625 41 444 1.51e-27 116
MsaG020832 AT4G08250.1 26.914 405 256 18 239 625 94 476 3.53e-23 103
MsaG020832 AT3G13840.1 23.310 429 273 17 223 625 111 509 1.81e-14 77.0
MsaG020832 AT5G67411.1 32.353 204 118 10 373 562 1 198 8.69e-13 68.2
MsaG020832 AT4G00150.1 24.251 367 241 12 265 625 218 553 2.14e-11 67.4

Find 139 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTCATCATATTGAAAT+TGG 0.107646 4:-59123927 None:intergenic
TTCGCTCTGTCTATTATTAA+TGG 0.122836 4:+59124877 MsaT020832.1:CDS
ACCCTTCATATCATTGATTT+TGG 0.132720 4:+59124487 MsaT020832.1:CDS
CTGAATTAATGGACAAATTT+AGG 0.141247 4:+59125154 MsaT020832.1:CDS
TTTCTTTGCTACACGGTTTA+AGG 0.151261 4:+59124915 MsaT020832.1:CDS
CTTCAAAACCTTTGTTGAAT+TGG 0.153592 4:-59123850 None:intergenic
GATGATTGAGAAAGAAATTA+TGG 0.215626 4:+59125011 MsaT020832.1:CDS
CTGGTGCTGGTTGTGCCATT+AGG 0.228538 4:-59123620 None:intergenic
AAAACAAAACGATAGATTTA+AGG 0.243289 4:+59124140 MsaT020832.1:CDS
TTCAAAACCTTTGTTGAATT+GGG 0.284127 4:-59123849 None:intergenic
GATTTGAGTTATGAACTTTA+TGG 0.285754 4:-59123510 None:intergenic
ATCCCAGTGATCCTCAATTT+TGG 0.286472 4:-59124574 None:intergenic
AAATGCAGAACTGCGTTTCT+AGG 0.293502 4:-59124829 None:intergenic
CATGATGCTCTTTCCCCTAA+TGG 0.300350 4:+59123605 MsaT020832.1:CDS
ACCGTTGAACTCTGAATTAA+TGG 0.311304 4:+59125143 MsaT020832.1:CDS
TGGTGCTGGTTGTGCCATTA+GGG 0.317958 4:-59123619 None:intergenic
TTTATATGCTTCAACTTGTT+GGG 0.327100 4:+59125260 MsaT020832.1:CDS
ACAGGAGTATCAGGTGAAAA+TGG 0.327130 4:-59123692 None:intergenic
ATGCCCTAGCGTCGCGGAAA+TGG 0.329797 4:+59124698 MsaT020832.1:CDS
GTTTCTGATCTTGTTAATCA+TGG 0.337520 4:+59123713 MsaT020832.1:CDS
ATTAGCATTTCTATTGTCAT+TGG 0.363634 4:-59124197 None:intergenic
AGATCAGAAACAGGAGTATC+AGG 0.366925 4:-59123701 None:intergenic
ACGCAGTTCTGCATTTGATT+AGG 0.369643 4:+59124836 MsaT020832.1:CDS
ATCCTCCATTCACTTCCCTT+TGG 0.370103 4:-59124988 None:intergenic
GGCCTTGAGGCTCGCCTTGT+AGG 0.370231 4:+59124298 MsaT020832.1:CDS
CTCTTCATCATATTGAAATT+GGG 0.378176 4:-59123926 None:intergenic
CATCAAATACAAAATCTCTA+TGG 0.379159 4:-59125197 None:intergenic
GAACCCTATAGAAGGTTATA+TGG 0.379462 4:+59123442 MsaT020832.1:CDS
CATATAGACAATGGCAGGTT+AGG 0.382866 4:+59125094 MsaT020832.1:CDS
AAATATGCAAATTTCTTGAA+TGG 0.386099 4:-59124421 None:intergenic
TGTTGAAACTCATCACAGTT+TGG 0.391044 4:-59123413 None:intergenic
GAAGCCATATAACCTTCTAT+AGG 0.392171 4:-59123446 None:intergenic
AAGCTAAGTGGGTCATAAAA+AGG 0.412714 4:-59123560 None:intergenic
AGGCAACATTCTTCACCCTT+TGG 0.417772 4:+59124238 MsaT020832.1:CDS
ATTATTATTGCTACTGGTGC+TGG 0.419180 4:-59123633 None:intergenic
TGCTTCAACTTGTTGGGTGC+CGG 0.420897 4:+59125266 MsaT020832.1:CDS
TGCCCTAGCGTCGCGGAAAT+GGG 0.427853 4:+59124699 MsaT020832.1:CDS
GGTACTATTATTATTGCTAC+TGG 0.432809 4:-59123639 None:intergenic
GGGATAGAGTTCCCCTTACC+AGG 0.440508 4:+59124592 MsaT020832.1:CDS
GTCTCCTCGATTCCTTCTGT+CGG 0.441440 4:-59124622 None:intergenic
TACCAAAATCAATGATATGA+AGG 0.442503 4:-59124489 None:intergenic
TTTCCCATTTCCGCGACGCT+AGG 0.446236 4:-59124702 None:intergenic
TTCACTTCCCTTTGGTATGA+CGG 0.446298 4:-59124980 None:intergenic
CCGCCAAAATTGAGGATCAC+TGG 0.447330 4:+59124571 MsaT020832.1:CDS
CTGCCATTGTCTATATGTCT+CGG 0.447892 4:-59125088 None:intergenic
AAGCCATATAACCTTCTATA+GGG 0.457326 4:-59123445 None:intergenic
CTGTGATTTGTAAGCTAAGT+GGG 0.457899 4:-59123571 None:intergenic
ATGATTGAGAAAGAAATTAT+GGG 0.460263 4:+59125012 MsaT020832.1:CDS
AATGACAATAGAAATGCTAA+TGG 0.460659 4:+59124199 MsaT020832.1:CDS
ACCTTTCCTTCGTTTGATGA+AGG 0.461817 4:-59124094 None:intergenic
CAATATGAGAACCCTATAGA+AGG 0.467769 4:+59123434 MsaT020832.1:CDS
CACCAGCTCTATCGCCTACA+AGG 0.469788 4:-59124312 None:intergenic
TTCTGCTATTTATGATATGT+TGG 0.469875 4:+59124954 MsaT020832.1:CDS
CCTTCTGTCGGGCGAAAGCC+TGG 0.470645 4:-59124610 None:intergenic
ATAGACAGAGCGAAAATATC+CGG 0.471744 4:-59124868 None:intergenic
TATTTGATGAAGACAGCAAA+TGG 0.482938 4:+59125211 MsaT020832.1:CDS
TGTCGGGCGAAAGCCTGGTA+AGG 0.486805 4:-59124605 None:intergenic
CGCCTTGTAGGCGATAGAGC+TGG 0.491595 4:+59124310 MsaT020832.1:CDS
TGCCATTGTCTATATGTCTC+GGG 0.492579 4:-59125087 None:intergenic
GACAGCAAATGGATGCTTCA+AGG 0.494379 4:+59125222 MsaT020832.1:CDS
CTGGCCACTGAAAACCATAA+AGG 0.503527 4:-59124513 None:intergenic
TTCCCATTTCCGCGACGCTA+GGG 0.504392 4:-59124701 None:intergenic
CGCCAAAATTGAGGATCACT+GGG 0.504465 4:+59124572 MsaT020832.1:CDS
GATAGAAACTTAATAAGCAC+TGG 0.505792 4:-59124532 None:intergenic
TTCATCTATTACCAATATGT+TGG 0.508354 4:+59123793 MsaT020832.1:CDS
TGATTAACAAGATCAGAAAC+AGG 0.510638 4:-59123710 None:intergenic
CTCAGCAGCAGTGATTCTCT+TGG 0.512609 4:-59124362 None:intergenic
AGGGAGAAATAACAAGCAAT+CGG 0.512691 4:+59123979 MsaT020832.1:CDS
GCAAATGGATGCTTCAAGGT+TGG 0.515939 4:+59125226 MsaT020832.1:CDS
TGATAAAGCTTTGCTTAATG+TGG 0.518577 4:+59124042 MsaT020832.1:CDS
ATGCAAATTTCTTGAATGGA+GGG 0.520263 4:-59124417 None:intergenic
TATGCAAATTTCTTGAATGG+AGG 0.522171 4:-59124418 None:intergenic
TCTCCTCGATTCCTTCTGTC+GGG 0.522739 4:-59124621 None:intergenic
AATGTTCTGTGCCAACATAT+TGG 0.522800 4:-59123804 None:intergenic
CTTTGTGATGCATCGCCAAA+GGG 0.524740 4:-59124253 None:intergenic
TTTGATTAGGAAGATAAGTC+CGG 0.526586 4:+59124849 MsaT020832.1:CDS
TTAAACCGTGTAGCAAAGAA+AGG 0.528289 4:-59124913 None:intergenic
GTAGTTGCTGTGAACTGTGT+GGG 0.528753 4:+59124763 MsaT020832.1:CDS
ACTAACACTACTTGAAGCAC+AGG 0.535170 4:-59123750 None:intergenic
CATGAGCGCGAAGAAGAAGA+AGG 0.535210 4:+59123959 MsaT020832.1:CDS
CAGGTTAGGAATACAAGAGC+TGG 0.537591 4:+59125108 MsaT020832.1:CDS
GTTCATAACTCAAATCCTCA+TGG 0.541442 4:+59123517 MsaT020832.1:CDS
TTTCCTTCGTTTGATGAAGG+TGG 0.542665 4:-59124091 None:intergenic
TGAGAAAGAAATTATGGGTA+GGG 0.543075 4:+59125017 MsaT020832.1:CDS
CCTCAGACTCAACTCTTGAA+AGG 0.543868 4:+59123893 MsaT020832.1:CDS
GTCGGGCGAAAGCCTGGTAA+GGG 0.545615 4:-59124604 None:intergenic
AAGATATGAATTGATTATGC+CGG 0.549732 4:-59125285 None:intergenic
TTTCTATCAAATAGAGAAGG+TGG 0.552509 4:+59124547 MsaT020832.1:CDS
CCAGGCTTTCGCCCGACAGA+AGG 0.554024 4:+59124610 MsaT020832.1:CDS
CCTTTGTGATGCATCGCCAA+AGG 0.561319 4:-59124254 None:intergenic
CGAGACATATAGACAATGGC+AGG 0.561554 4:+59125089 MsaT020832.1:CDS
TTGAGAAAGAAATTATGGGT+AGG 0.562013 4:+59125016 MsaT020832.1:CDS
GTGGCTCATTACTTTGCCGA+TGG 0.563540 4:+59124277 MsaT020832.1:CDS
TCATACCAAAGGGAAGTGAA+TGG 0.566189 4:+59124983 MsaT020832.1:CDS
TCTGTGATTTGTAAGCTAAG+TGG 0.568670 4:-59123572 None:intergenic
TCTATTTCCCAATTCAACAA+AGG 0.569524 4:+59123842 MsaT020832.1:CDS
ATAGTTAGCAAGTCGATGAC+CGG 0.571078 4:-59124644 None:intergenic
CGCCTACAAGGCGAGCCTCA+AGG 0.571405 4:-59124300 None:intergenic
ACCAAAATCAATGATATGAA+GGG 0.575569 4:-59124488 None:intergenic
AAGGCGAGCCTCAAGGCCAT+CGG 0.575761 4:-59124293 None:intergenic
ACAGAAGGAATCGAGGAGAC+CGG 0.577833 4:+59124625 MsaT020832.1:CDS
CTTTGGCGATGCATCACAAA+GGG 0.583245 4:+59124255 MsaT020832.1:CDS
GCAGTGATTCTCTTGGTGCT+AGG 0.583817 4:-59124355 None:intergenic
ATTCACCTTTCTTTGCTACA+CGG 0.584416 4:+59124908 MsaT020832.1:CDS
TTACTTTGCCGATGGCCTTG+AGG 0.590932 4:+59124285 MsaT020832.1:CDS
ATGAATGTTGTAGCATGTGA+AGG 0.591903 4:+59125045 MsaT020832.1:CDS
GTTGGACGCCGTCATACCAA+AGG 0.593292 4:+59124972 MsaT020832.1:CDS
CTTTGCTACACGGTTTAAGG+AGG 0.595296 4:+59124918 MsaT020832.1:CDS
CCTTTCAAGAGTTGAGTCTG+AGG 0.595634 4:-59123893 None:intergenic
TGGATGCTTCAAGGTTGGAA+AGG 0.599055 4:+59125231 MsaT020832.1:CDS
GGCTATTAGTGTTGTTGATG+AGG 0.599082 4:+59124000 MsaT020832.1:CDS
TTGGACGCCGTCATACCAAA+GGG 0.599381 4:+59124973 MsaT020832.1:CDS
ATGAGCGCGAAGAAGAAGAA+GGG 0.599909 4:+59123960 MsaT020832.1:CDS
TTCAAGAGTTGAGTCTGAGG+AGG 0.601098 4:-59123890 None:intergenic
AAGGTGGACCGCCAAAATTG+AGG 0.602746 4:+59124563 MsaT020832.1:CDS
TCCATTAATTCAGAGTTCAA+CGG 0.603223 4:-59125144 None:intergenic
TTAGGACTAAGTTAAAGCAA+TGG 0.607899 4:+59125172 MsaT020832.1:CDS
GATTCTCTTGGTGCTAGGCG+AGG 0.610614 4:-59124350 None:intergenic
TTCAGTGACAGATTCTTCCA+TGG 0.614881 4:+59123466 MsaT020832.1:CDS
GCAAGAATCGTCGATGCTAG+TGG 0.620710 4:-59123660 None:intergenic
TTGCTGTGAACTGTGTGGGG+AGG 0.623836 4:+59124767 MsaT020832.1:CDS
CATTAGGGGAAAGAGCATCA+TGG 0.624005 4:-59123604 None:intergenic
ACCTTCATCAAACGAAGGAA+AGG 0.627171 4:+59124093 MsaT020832.1:CDS
TGGCGATGCATCACAAAGGG+TGG 0.631669 4:+59124258 MsaT020832.1:CDS
AAGTTTCTATCAAATAGAGA+AGG 0.631857 4:+59124544 MsaT020832.1:CDS
GGTGCTGGTTGTGCCATTAG+GGG 0.631907 4:-59123618 None:intergenic
CCTTTGGCGATGCATCACAA+AGG 0.636123 4:+59124254 MsaT020832.1:CDS
GCTTCAAGTAGTGTTAGTGA+TGG 0.644911 4:+59123755 MsaT020832.1:CDS
TAGTTGCTGTGAACTGTGTG+GGG 0.647970 4:+59124764 MsaT020832.1:CDS
GGCCCGAGACATATAGACAA+TGG 0.648575 4:+59125085 MsaT020832.1:CDS
TCGGGCGAAAGCCTGGTAAG+GGG 0.648782 4:-59124603 None:intergenic
TCGCCCGACAGAAGGAATCG+AGG 0.651016 4:+59124618 MsaT020832.1:CDS
AAGGTTTAGAAAGAGTTGAG+AGG 0.653367 4:+59125064 MsaT020832.1:CDS
GCTAGGGCATTGTACTCGAA+CGG 0.658627 4:-59124685 None:intergenic
TACCAAAGGGAAGTGAATGG+AGG 0.666797 4:+59124986 MsaT020832.1:CDS
AGTACAATGCCCTAGCGTCG+CGG 0.670515 4:+59124692 MsaT020832.1:CDS
GGTAGTTGCTGTGAACTGTG+TGG 0.682353 4:+59124762 MsaT020832.1:CDS
CAACCACCTTCATCAAACGA+AGG 0.691308 4:+59124088 MsaT020832.1:CDS
TCTTAAAATCAAGAGCTACG+AGG 0.741825 4:+59124741 MsaT020832.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 59123407 59125293 59123407 ID=MsaG020832
Chr4 mRNA 59123407 59125293 59123407 ID=MsaT020832.1;Parent=MsaG020832
Chr4 exon 59123407 59125293 59123407 ID=MsaT020832.1.exon1;Parent=MsaT020832.1
Chr4 CDS 59123407 59125293 59123407 ID=cds.MsaT020832.1;Parent=MsaT020832.1
Gene Sequence

>MsaT020832.1

ATGGATCCAAACTGTGATGAGTTTCAACAATATGAGAACCCTATAGAAGGTTATATGGCTTCAGTGACAGATTCTTCCATGGAAGACACTGATTTTTCAGAAACCATAAAGTTCATAACTCAAATCCTCATGGAAGAAAACGTTGACCAGAGACCTTTTTATGACCCACTTAGCTTACAAATCACAGAAAAATCATTCCATGATGCTCTTTCCCCTAATGGCACAACCAGCACCAGTAGCAATAATAATAGTACCACTAGCATCGACGATTCTTGCAAGTCTAAACCATTTTCACCTGATACTCCTGTTTCTGATCTTGTTAATCATGGTTTTCAGTTTAATTCCTGTGCTTCAAGTAGTGTTAGTGATGGACTCGTAAATTTCGATTCATCTATTACCAATATGTTGGCACAGAACATTTTCAGTAATGCAGATTCTATTTCCCAATTCAACAAAGGTTTTGAAGAAGCTAACAAGTTTCTTCCTCCTCAGACTCAACTCTTGAAAGGTTTAGAATCACCCAATTTCAATATGATGAAGAGTAGAAAGAATCATGAGCGCGAAGAAGAAGAAGGGAGAAATAACAAGCAATCGGCTATTAGTGTTGTTGATGAGGATGAATTATCAGAAATGTTTGATAAAGCTTTGCTTAATGTGGACCGTGAATTTTTGCAGAATGAGCAACCACCTTCATCAAACGAAGGAAAGGTTCGTCCAAAGAAACGTGATAGCAAAAACAAAACGATAGATTTAAGGAATCTTCTGCTTATGTGTTCACAAGCTATGTATGCCAATGACAATAGAAATGCTAATGGTCTTTTAAAGCAGATTAGGCAACATTCTTCACCCTTTGGCGATGCATCACAAAGGGTGGCTCATTACTTTGCCGATGGCCTTGAGGCTCGCCTTGTAGGCGATAGAGCTGGTGCGCAAACATTCTATTCCTCGCCTAGCACCAAGAGAATCACTGCTGCTGAGTTTTTGAAAGCATATCAAGTTCATTTTACTAGCCCTCCATTCAAGAAATTTGCATATTTGTTTGCAAATGAAATGATTATGAAAGTTGCTGCAAAAGCTGAAACCCTTCATATCATTGATTTTGGTATCCTTTATGGTTTTCAGTGGCCAGTGCTTATTAAGTTTCTATCAAATAGAGAAGGTGGACCGCCAAAATTGAGGATCACTGGGATAGAGTTCCCCTTACCAGGCTTTCGCCCGACAGAAGGAATCGAGGAGACCGGTCATCGACTTGCTAACTATTGCAAGCGTTTCAATGTTCCGTTCGAGTACAATGCCCTAGCGTCGCGGAAATGGGAAACAATTCGAGTTAAAGATCTTAAAATCAAGAGCTACGAGGTAGTTGCTGTGAACTGTGTGGGGAGGTTTAAGAATTTGATAGATGAGAGTATTGAAGTTAATAGTCCTAGAAACGCAGTTCTGCATTTGATTAGGAAGATAAGTCCGGATATTTTCGCTCTGTCTATTATTAATGGATCATATAATTCACCTTTCTTTGCTACACGGTTTAAGGAGGCACTGTTTAATTTTTCTGCTATTTATGATATGTTGGACGCCGTCATACCAAAGGGAAGTGAATGGAGGATGATGATTGAGAAAGAAATTATGGGTAGGGAAGTTATGAATGTTGTAGCATGTGAAGGTTTAGAAAGAGTTGAGAGGCCCGAGACATATAGACAATGGCAGGTTAGGAATACAAGAGCTGGTTTCAAGCAGCTACCGTTGAACTCTGAATTAATGGACAAATTTAGGACTAAGTTAAAGCAATGGTACCATAGAGATTTTGTATTTGATGAAGACAGCAAATGGATGCTTCAAGGTTGGAAAGGTCGTATTTTATATGCTTCAACTTGTTGGGTGCCGGCATAA
Protein sequence

>MsaT020832.1

MDPNCDEFQQYENPIEGYMASVTDSSMEDTDFSETIKFITQILMEENVDQRPFYDPLSLQITEKSFHDALSPNGTTSTSSNNNSTTSIDDSCKSKPFSPDTPVSDLVNHGFQFNSCASSSVSDGLVNFDSSITNMLAQNIFSNADSISQFNKGFEEANKFLPPQTQLLKGLESPNFNMMKSRKNHEREEEEGRNNKQSAISVVDEDELSEMFDKALLNVDREFLQNEQPPSSNEGKVRPKKRDSKNKTIDLRNLLLMCSQAMYANDNRNANGLLKQIRQHSSPFGDASQRVAHYFADGLEARLVGDRAGAQTFYSSPSTKRITAAEFLKAYQVHFTSPPFKKFAYLFANEMIMKVAAKAETLHIIDFGILYGFQWPVLIKFLSNREGGPPKLRITGIEFPLPGFRPTEGIEETGHRLANYCKRFNVPFEYNALASRKWETIRVKDLKIKSYEVVAVNCVGRFKNLIDESIEVNSPRNAVLHLIRKISPDIFALSIINGSYNSPFFATRFKEALFNFSAIYDMLDAVIPKGSEWRMMIEKEIMGREVMNVVACEGLERVERPETYRQWQVRNTRAGFKQLPLNSELMDKFRTKLKQWYHRDFVFDEDSKWMLQGWKGRILYASTCWVPA*