AlfalfaGEDB | MsaG020833

M. sativa ssp. caerulea / MsaG020833

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03514.17

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020833	XP_003606669.1	94.830	735	35	1	1	732	1	735	0.0	1406

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020833	sp\|Q9XE58\|SCL14_ARATH	47.293	702	286	18	93	732	88	767	0.0	578

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020833	tr\|G7JUT6\|G7JUT6_MEDTR	94.830	735	35	1	1	732	1	735	0.0	1406

TFs/TRs:

Gene ID	Type	Classification
MsaG020833	TF	GRAS

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000118	MsaG020833	0.808871	4.267454e-50	7.091946e-48
MsaG000248	MsaG020833	0.800927	1.917155e-48	2.646887e-46
MsaG001381	MsaG020833	0.809577	3.016205e-50	5.098706e-48
MsaG001386	MsaG020833	0.805514	2.177134e-49	3.340173e-47
MsaG001390	MsaG020833	0.831652	2.669149e-55	8.101813e-53
MsaG002496	MsaG020833	0.809087	3.838649e-50	6.412486e-48
MsaG003509	MsaG020833	0.809978	2.475120e-50	4.224535e-48
MsaG004084	MsaG020833	0.829719	7.898356e-55	2.267508e-52
MsaG004734	MsaG020833	0.826322	5.144829e-54	1.342415e-51
MsaG004902	MsaG020833	0.804937	2.870457e-49	4.345415e-47
MsaG006760	MsaG020833	0.883968	6.138402e-71	1.337499e-67
MsaG009121	MsaG020833	0.806608	1.284168e-49	2.021818e-47
MsaG010497	MsaG020833	0.819930	1.570174e-52	3.446946e-50
MsaG012081	MsaG020833	0.826711	4.161269e-54	1.097623e-51
MsaG012189	MsaG020833	0.847680	1.874454e-59	9.390924e-57
MsaG012498	MsaG020833	0.806627	1.272901e-49	2.004950e-47
MsaG012636	MsaG020833	0.811330	1.267117e-50	2.235340e-48
MsaG015993	MsaG020833	0.802413	9.534898e-49	1.361683e-46
MsaG016406	MsaG020833	0.810410	1.999684e-50	3.449304e-48
MsaG016550	MsaG020833	0.806497	1.355073e-49	2.127846e-47
MsaG017371	MsaG020833	0.818260	3.750209e-52	7.880090e-50
MsaG018676	MsaG020833	0.823080	2.963641e-53	7.075910e-51
MsaG018897	MsaG020833	0.801722	1.320516e-48	1.856290e-46
MsaG019118	MsaG020833	0.817061	6.971017e-52	1.420257e-49
MsaG019122	MsaG020833	0.821226	7.936687e-53	1.803153e-50
MsaG020243	MsaG020833	0.841060	1.106661e-57	4.466849e-55
MsaG020833	MsaG023689	0.812554	6.878329e-51	1.250579e-48
MsaG020833	MsaG027237	0.808748	4.531768e-50	7.509156e-48
MsaG020833	MsaG027546	0.803228	6.483489e-49	9.434087e-47
MsaG020833	MsaG027579	0.817090	6.866334e-52	1.399990e-49
MsaG020833	MsaG028258	0.818939	2.634784e-52	5.635305e-50
MsaG020833	MsaG028620	0.812018	8.989973e-51	1.612956e-48
MsaG020833	MsaG028754	0.810681	1.748765e-50	3.036297e-48
MsaG020833	MsaG029306	0.801566	1.420887e-48	1.990296e-46
MsaG020833	MsaG030179	0.843863	2.015457e-58	8.896654e-56
MsaG020833	MsaG033513	0.820747	1.021879e-52	2.292283e-50
MsaG020833	MsaG035522	0.800178	2.721379e-48	3.694115e-46
MsaG020833	MsaG042697	0.802727	8.219047e-49	1.182196e-46
MsaG020833	MsaG046203	0.806281	1.504329e-49	2.350198e-47
MsaG020833	MsaG046459	0.803633	5.349187e-49	7.856553e-47
MsaG020833	MsaG046782	0.813379	4.544236e-51	8.433842e-49
MsaG020833	MsaG046931	0.800764	2.069595e-48	2.846950e-46
MsaG020833	MsaG002584	0.806032	1.696113e-49	2.634100e-47
MsaG020833	MsaG007870	0.833843	7.672976e-56	2.482783e-53
MsaG020833	MsaG013380	0.825153	9.712670e-54	2.453499e-51
MsaG020833	MsaG032938	0.803307	6.244372e-49	9.102613e-47
MsaG020833	MsaG032354	0.805096	2.660600e-49	4.042583e-47
MsaG020833	MsaG031490	0.819001	2.551145e-52	5.465234e-50
MsaG020833	MsaG032445	0.818155	3.959557e-52	8.297741e-50
MsaG020833	MsaG031607	0.800215	2.674782e-48	3.633894e-46
MsaG020833	MsaG030125	0.801970	1.175149e-48	1.661278e-46
MsaG020833	MsaG036797	0.839269	3.231124e-57	1.232756e-54
MsaG020833	MsaG041255	0.803904	4.702433e-49	6.950121e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020833	MtrunA17_Chr4g0031711	94.830	735	35	1	1	732	1	735	0.0	1406
MsaG020833	MtrunA17_Chr4g0031701	72.139	664	135	7	70	732	14	628	0.0	933
MsaG020833	MtrunA17_Chr4g0031731	71.837	664	137	8	70	732	14	628	0.0	921
MsaG020833	MtrunA17_Chr4g0031721	49.277	692	281	13	80	732	26	686	0.0	610
MsaG020833	MtrunA17_Chr7g0238891	45.152	691	329	17	80	732	64	742	0.0	547
MsaG020833	MtrunA17_Chr4g0031741	52.446	511	219	9	236	732	152	652	9.23e-174	513
MsaG020833	MtrunA17_Chr2g0328411	44.745	666	294	19	83	730	45	654	1.53e-173	513
MsaG020833	MtrunA17_Chr2g0328431	44.745	666	298	17	83	732	45	656	1.88e-173	512
MsaG020833	MtrunA17_Chr2g0328341	48.326	478	230	7	257	732	178	640	7.01e-158	471
MsaG020833	MtrunA17_Chr2g0328401	42.925	629	299	15	93	710	56	635	3.98e-153	459
MsaG020833	MtrunA17_Chr2g0328381	47.817	458	220	8	280	732	200	643	3.23e-149	449
MsaG020833	MtrunA17_Chr2g0328361	48.402	438	187	8	296	732	218	617	8.72e-138	419
MsaG020833	MtrunA17_Chr2g0328371	52.157	255	116	3	432	685	2	251	5.82e-92	287
MsaG020833	MtrunA17_Chr2g0319091	32.979	376	247	4	354	729	208	578	4.62e-68	234
MsaG020833	MtrunA17_Chr5g0447691	32.181	376	250	3	354	729	173	543	2.01e-63	221
MsaG020833	MtrunA17_Chr3g0101141	32.804	378	245	6	354	729	171	541	8.73e-61	213
MsaG020833	MtrunA17_Chr2g0290241	29.894	378	259	4	353	729	52	424	6.62e-60	208
MsaG020833	MtrunA17_Chr4g0076091	30.829	386	247	8	353	730	170	543	2.21e-57	204
MsaG020833	MtrunA17_Chr5g0445331	31.865	386	242	9	354	729	157	531	8.12e-57	202
MsaG020833	MtrunA17_Chr1g0159461	28.877	374	261	3	356	729	223	591	9.22e-54	195
MsaG020833	MtrunA17_Chr8g0357601	30.479	397	246	7	350	732	174	554	9.27e-54	194
MsaG020833	MtrunA17_Chr7g0224271	30.923	401	240	7	348	729	290	672	1.08e-53	196
MsaG020833	MtrunA17_Chr3g0125811	28.877	374	261	3	356	729	201	569	1.29e-53	194
MsaG020833	MtrunA17_Chr4g0057891	29.899	398	248	8	347	729	138	519	3.76e-53	192
MsaG020833	MtrunA17_Chr3g0110971	30.435	414	251	8	333	729	193	586	8.95e-50	184
MsaG020833	MtrunA17_Chr2g0306641	30.412	388	248	6	348	729	115	486	1.47e-49	181
MsaG020833	MtrunA17_Chr8g0376381	31.266	387	227	10	353	729	164	521	1.18e-46	174
MsaG020833	MtrunA17_Chr7g0245601	30.270	370	240	7	362	731	442	793	1.45e-46	177
MsaG020833	MtrunA17_Chr4g0038851	27.293	458	261	9	334	729	21	468	1.77e-45	169
MsaG020833	MtrunA17_Chr2g0294461	29.873	395	254	9	348	729	200	584	1.40e-43	166
MsaG020833	MtrunA17_Chr4g0038761	28.302	371	252	5	362	732	88	444	2.81e-43	162
MsaG020833	MtrunA17_Chr5g0396491	27.356	435	246	10	355	729	53	477	3.69e-41	157
MsaG020833	MtrunA17_Chr3g0085681	26.366	421	249	8	359	729	26	435	9.53e-41	155
MsaG020833	MtrunA17_Chr4g0057291	26.804	388	262	10	354	731	288	663	1.37e-39	155
MsaG020833	MtrunA17_Chr2g0294471	27.990	393	261	9	348	729	194	575	3.17e-36	144
MsaG020833	MtrunA17_Chr1g0183641	27.763	389	245	14	350	729	115	476	6.33e-36	142
MsaG020833	MtrunA17_Chr1g0200401	30.250	400	236	14	355	729	77	458	9.33e-35	138
MsaG020833	MtrunA17_Chr3g0114841	26.799	403	263	10	347	729	109	499	2.11e-33	135
MsaG020833	MtrunA17_Chr5g0401131	25.916	382	273	5	357	731	113	491	1.57e-32	132
MsaG020833	MtrunA17_Chr4g0039661	27.198	364	239	9	374	729	209	554	2.31e-32	132
MsaG020833	MtrunA17_Chr5g0401421	23.709	426	268	11	355	732	32	448	1.06e-30	125
MsaG020833	MtrunA17_Chr5g0424131	28.499	393	246	12	359	730	121	499	1.37e-30	126
MsaG020833	MtrunA17_Chr7g0270031	28.205	390	257	12	348	729	179	553	1.61e-30	127
MsaG020833	MtrunA17_Chr7g0236851	25.069	363	251	7	374	730	310	657	1.88e-30	127
MsaG020833	MtrunA17_Chr4g0053901	24.582	419	271	9	355	732	56	470	2.47e-30	125
MsaG020833	MtrunA17_Chr6g0468561	26.289	388	253	12	356	729	293	661	9.95e-30	125
MsaG020833	MtrunA17_Chr1g0194621	26.434	401	257	13	348	729	136	517	9.44e-29	121
MsaG020833	MtrunA17_Chr8g0383981	26.895	409	246	17	343	732	124	498	2.12e-28	120
MsaG020833	MtrunA17_Chr3g0085361	25.070	355	208	7	424	729	4	349	1.59e-27	114
MsaG020833	MtrunA17_Chr2g0329591	25.667	300	213	4	358	656	74	364	7.34e-22	99.4
MsaG020833	MtrunA17_Chr2g0328391	42.405	158	62	9	1	153	1	134	1.66e-18	90.1
MsaG020833	MtrunA17_Chr7g0230731	23.077	403	275	8	335	729	352	727	1.31e-15	80.9
MsaG020833	MtrunA17_Chr7g0230771	22.475	396	286	7	335	729	355	730	2.01e-15	80.5
MsaG020833	MtrunA17_Chr4g0012071	24.000	375	260	9	359	729	197	550	1.31e-14	77.4
MsaG020833	MtrunA17_Chr8g0344101	24.352	386	233	14	358	716	223	576	2.50e-14	76.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG020833	AT1G07530.1	47.293	702	286	18	93	732	88	767	0.0	578
MsaG020833	AT2G29060.2	47.793	657	280	15	85	731	53	656	0.0	563
MsaG020833	AT2G29060.1	47.605	668	302	14	85	731	53	693	0.0	558
MsaG020833	AT2G29065.1	45.776	651	283	15	93	732	39	630	0.0	535
MsaG020833	AT1G07520.2	51.136	528	240	8	211	732	16	531	4.85e-179	522
MsaG020833	AT1G07520.4	51.136	528	240	8	211	732	16	531	4.85e-179	522
MsaG020833	AT1G07520.1	46.037	656	307	12	93	732	71	695	7.59e-179	528
MsaG020833	AT1G07520.3	46.037	656	307	12	93	732	72	696	8.36e-179	528
MsaG020833	AT2G37650.2	43.215	678	326	18	80	731	58	702	1.08e-167	499
MsaG020833	AT2G37650.1	43.215	678	326	18	80	731	70	714	1.29e-167	499
MsaG020833	AT3G46600.3	50.111	451	208	5	282	732	116	549	1.76e-152	455
MsaG020833	AT3G46600.1	50.111	451	208	5	282	732	148	581	2.10e-152	456
MsaG020833	AT5G59450.1	47.708	480	232	7	261	732	132	600	2.75e-149	449
MsaG020833	AT3G46600.2	52.910	378	173	2	355	732	1	373	1.11e-141	421
MsaG020833	AT2G04890.1	32.181	376	243	4	354	729	49	412	1.93e-60	209
MsaG020833	AT1G50600.3	32.181	376	250	3	354	729	155	525	4.73e-60	211
MsaG020833	AT1G50600.1	32.181	376	250	3	354	729	226	596	2.63e-59	211
MsaG020833	AT1G50600.2	32.181	376	250	3	354	729	230	600	2.98e-59	211
MsaG020833	AT5G48150.1	30.585	376	257	3	354	729	118	489	1.37e-57	204
MsaG020833	AT5G48150.4	30.585	376	257	3	354	729	118	489	1.37e-57	204
MsaG020833	AT5G48150.2	30.585	376	257	3	354	729	118	489	1.37e-57	204
MsaG020833	AT5G48150.3	30.585	376	257	3	354	729	118	489	1.37e-57	204
MsaG020833	AT1G21450.1	30.585	376	253	5	356	729	223	592	3.54e-55	199
MsaG020833	AT4G17230.1	30.918	414	276	5	322	731	119	526	4.64e-53	192
MsaG020833	AT4G17230.2	30.918	414	276	5	322	731	119	526	4.64e-53	192
MsaG020833	AT1G66350.1	31.234	381	233	10	353	729	150	505	3.16e-48	178
MsaG020833	AT1G50420.1	25.694	432	257	5	355	729	54	478	6.71e-47	174
MsaG020833	AT3G54220.1	30.280	393	257	8	340	731	275	651	8.75e-47	177
MsaG020833	AT2G01570.1	30.208	384	239	10	353	729	219	580	1.29e-46	175
MsaG020833	AT1G14920.1	30.366	382	241	9	353	729	167	528	2.40e-46	173
MsaG020833	AT3G03450.1	28.721	383	251	9	353	729	178	544	3.11e-46	173
MsaG020833	AT5G17490.1	26.190	420	280	10	317	729	119	515	1.43e-42	162
MsaG020833	AT5G41920.1	28.346	381	252	6	352	729	37	399	1.06e-41	157
MsaG020833	AT5G66770.1	26.914	405	278	8	330	729	192	583	1.99e-37	148
MsaG020833	AT5G52510.1	26.410	390	255	10	356	729	266	639	1.38e-36	146
MsaG020833	AT1G63100.2	31.231	333	215	8	354	683	273	594	2.73e-36	145
MsaG020833	AT1G63100.1	31.231	333	215	8	354	683	248	569	2.99e-36	145
MsaG020833	AT4G37650.1	25.445	393	268	10	357	731	145	530	1.76e-34	139
MsaG020833	AT3G50650.1	29.283	321	208	5	367	681	184	491	1.09e-33	136
MsaG020833	AT3G49950.1	26.854	391	264	10	355	731	27	409	2.56e-30	124
MsaG020833	AT1G55580.1	25.060	419	255	11	355	729	41	444	4.98e-26	112
MsaG020833	AT4G08250.1	26.700	397	261	14	348	729	95	476	1.07e-25	112
MsaG020833	AT3G13840.1	23.256	430	254	16	328	729	128	509	5.29e-15	79.0
MsaG020833	AT4G00150.1	24.403	377	246	12	360	729	209	553	2.29e-11	67.4

Find 167 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGGAATTAATGGACAAATTT+AGG	0.087513	4:-59128864	MsaT020833.1:CDS
GATGAGATTTAAGAATTTAT+TGG	0.142030	4:-59129235	MsaT020833.1:CDS
ACCCTCCATATCATTGATTT+TGG	0.161875	4:-59129531	MsaT020833.1:CDS
TCCATTGAATTCGGAATTAA+TGG	0.174492	4:-59128875	MsaT020833.1:CDS
AATCAATATGATAGCTTTCT+TGG	0.191887	4:-59130773	MsaT020833.1:CDS
ATGCCATACCATCGCGTTAT+TGG	0.192133	4:-59129320	MsaT020833.1:CDS
CATTCTTTGCTACACGGTTT+AGG	0.223208	4:-59129104	MsaT020833.1:CDS
AAATGCAGAACCGCATTTCT+AGG	0.241890	4:+59129189	None:intergenic
CTCCCTAACCATATCCAAAT+TGG	0.268685	4:+59130178	None:intergenic
TTTGTTCAGTCAATTGTTAA+TGG	0.288838	4:-59129141	MsaT020833.1:CDS
ATCAGAGTTATGAATATCTA+AGG	0.292408	4:+59130508	None:intergenic
TGAATCATCCAATTTGGATA+TGG	0.301149	4:-59130186	MsaT020833.1:CDS
CAAACAGCTTCCATTGAATT+CGG	0.304465	4:-59128884	MsaT020833.1:CDS
ATTCTTTGCTACACGGTTTA+GGG	0.306681	4:-59129103	MsaT020833.1:CDS
TATCTAAGGGGTGTTGATTA+GGG	0.313284	4:+59130522	None:intergenic
TCACTAGCTTGAGGAAAGTT+TGG	0.320173	4:+59130917	None:intergenic
ATATCTAAGGGGTGTTGATT+AGG	0.323460	4:+59130521	None:intergenic
CATCCAATTTGGATATGGTT+AGG	0.330014	4:-59130181	MsaT020833.1:CDS
TGCCATACCATCGCGTTATT+GGG	0.330847	4:-59129319	MsaT020833.1:CDS
ACTCTATCCCGGTGATCTTC+AGG	0.333841	4:+59129439	None:intergenic
GGATACCAAAATCAATGATA+TGG	0.351602	4:+59129526	None:intergenic
CGAGTCTTCCCTCCATTTGA+TGG	0.357746	4:+59129921	None:intergenic
TGATCAGAAACAGGGGTATC+AGG	0.360045	4:+59130416	None:intergenic
AGAAAGCTATCATATTGATT+TGG	0.360772	4:+59130776	None:intergenic
TTTATATGCTTCCACATGTT+GGG	0.369926	4:-59128758	MsaT020833.1:CDS
CATATAAACAGTGGCAGGTT+AGG	0.372839	4:-59128924	MsaT020833.1:CDS
ATTGCTATTGCTGTTGCTGC+TGG	0.378194	4:+59130478	None:intergenic
GGCCTTGAGGCACGCCTTGT+CGG	0.379088	4:-59129720	MsaT020833.1:CDS
CTTCCCTATAGAAGGTTATA+TGG	0.379175	4:-59130720	MsaT020833.1:CDS
ATTAGCATTCCTGTTGTCAT+TGG	0.384143	4:+59129821	None:intergenic
CGCCTTGTCGGCGATGGTAC+TGG	0.387590	4:-59129708	MsaT020833.1:CDS
ATTTCTCAACTTCATTCTAC+TGG	0.395432	4:-59130359	MsaT020833.1:CDS
TAGTGATGGACTCAGAGATT+TGG	0.396758	4:-59130333	MsaT020833.1:CDS
ATCCCGGTGATCTTCAGGTT+TGG	0.401423	4:+59129444	None:intergenic
AGGAAGCTATCATATTCATT+TGG	0.404865	4:+59130809	None:intergenic
TGATTAACATGATCAGAAAC+AGG	0.407330	4:+59130407	None:intergenic
ATTATCAGAGATGTTTGATA+AGG	0.416031	4:-59130027	MsaT020833.1:CDS
AGAGAATCCGTTTGTGTTGA+AGG	0.418700	4:-59130153	MsaT020833.1:CDS
AGGTAGCTATCATATTGATT+TGG	0.419337	4:+59130842	None:intergenic
TCCATTAATTCCGAATTCAA+TGG	0.422266	4:+59128874	None:intergenic
GAGGCCATATAACCTTCTAT+AGG	0.431388	4:+59130716	None:intergenic
CTGCCACTGTTTATATGTCT+CGG	0.432575	4:+59128930	None:intergenic
ATGAGCTACTGAAGCAGATT+AGG	0.435797	4:-59129800	MsaT020833.1:CDS
GCCACCACCTTCCCTATAGA+AGG	0.439615	4:-59130728	MsaT020833.1:CDS
AGGCAACATTCTTCACCCTC+TGG	0.447707	4:-59129780	MsaT020833.1:CDS
CTGTATATGCCAATGACAAC+AGG	0.450042	4:-59129830	MsaT020833.1:CDS
AGAATGAAACAATAGATTTG+AGG	0.453249	4:-59129878	MsaT020833.1:CDS
GGCAACATTCTTCACCCTCT+GGG	0.453926	4:-59129779	MsaT020833.1:CDS
ATGGAAAGAATCGAAGAAAC+TGG	0.455010	4:-59129393	MsaT020833.1:CDS
TTTCTTGGTGCTGATCCACT+TGG	0.459039	4:-59130758	MsaT020833.1:CDS
GTTTCTTCGATTCTTTCCAT+TGG	0.459734	4:+59129396	None:intergenic
TTCAAGAAAAGTTTAGAAGA+AGG	0.460061	4:-59130251	MsaT020833.1:CDS
AGGCCATATAACCTTCTATA+GGG	0.460382	4:+59130717	None:intergenic
ATCCAATTTGGATATGGTTA+GGG	0.463279	4:-59130180	MsaT020833.1:CDS
TTTCTACCCTTCAACACAAA+CGG	0.464697	4:+59130146	None:intergenic
GTGGTAACATTGCTGTAACT+TGG	0.472903	4:+59128840	None:intergenic
AGGTTATATGGCCTCGGCGA+CGG	0.474767	4:-59130708	MsaT020833.1:CDS
GACAACAATTGGATGCTTCA+AGG	0.476004	4:-59128796	MsaT020833.1:CDS
GGGATAGAGTTCCCTTTACC+TGG	0.477006	4:-59129426	MsaT020833.1:CDS
GTAGAGATCCTCTTGGTGTT+CGG	0.479560	4:+59129663	None:intergenic
GAATGAAGTTGAGAAATAGC+AGG	0.479774	4:+59130365	None:intergenic
AAGAACAAGAAGAAGAAGCT+TGG	0.480178	4:-59130094	MsaT020833.1:CDS
TGCCACTGTTTATATGTCTC+GGG	0.483709	4:+59128931	None:intergenic
CCGCAGCCTCAACTCTTGAC+TGG	0.485147	4:-59130212	MsaT020833.1:CDS
GAGGAGTCACCTCCATCAAA+TGG	0.486751	4:-59129933	MsaT020833.1:CDS
TTGGCTCATTACTTTGCCAA+TGG	0.490767	4:-59129741	MsaT020833.1:CDS
TTGGACGAAAACCAGGTAAA+GGG	0.491920	4:+59129415	None:intergenic
ACAATTGGATGCTTCAAGGT+TGG	0.492282	4:-59128792	MsaT020833.1:CDS
GGGTGTTGATTAGGGGAAAG+AGG	0.492324	4:+59130530	None:intergenic
CTTTCCATTGGACGAAAACC+AGG	0.495527	4:+59129408	None:intergenic
AACAGGGGTATCAGGTGAAA+GGG	0.498413	4:+59130424	None:intergenic
CTGTCATTTGCAAGCTAAGT+GGG	0.500777	4:+59130591	None:intergenic
GAAAATGATTCCTTGCAGAA+TGG	0.503064	4:-59129969	MsaT020833.1:CDS
TTGGCCACTGAAAACCATAT+AGG	0.503360	4:+59129505	None:intergenic
TGGGGATGGACCACAGAGGT+TGG	0.505585	4:-59129760	MsaT020833.1:CDS
CCGGTGATCTTCAGGTTTGG+AGG	0.507786	4:+59129447	None:intergenic
TTCCCAATAACGCGATGGTA+TGG	0.510255	4:+59129317	None:intergenic
ACTAACCACTTTGGTCAAGA+AGG	0.510864	4:-59130890	MsaT020833.1:CDS
GCAGAATGGACAAGTGAAAA+CGG	0.510872	4:-59129955	MsaT020833.1:CDS
AAGGCGTGCCTCAAGGCCAT+TGG	0.511609	4:+59129725	None:intergenic
AGTGATTTCACTAACCACTT+TGG	0.512697	4:-59130899	MsaT020833.1:CDS
TATAGAAGGTTATATGGCCT+CGG	0.515236	4:-59130714	MsaT020833.1:CDS
CATTCTTCACCCTCTGGGGA+TGG	0.516297	4:-59129774	MsaT020833.1:CDS
TTGGGAAACAATTCGAGTTG+AGG	0.525214	4:-59129301	MsaT020833.1:CDS
CGCCGACAAGGCGTGCCTCA+AGG	0.525318	4:+59129718	None:intergenic
CTAAACCGTGTAGCAAAGAA+TGG	0.525919	4:+59129105	None:intergenic
ATTCATTTGGATCAGCATCA+AGG	0.527139	4:+59130822	None:intergenic
GGATTCTTCTATTACACAGA+TGG	0.528072	4:-59130312	MsaT020833.1:CDS
GGTATGGCATTAAACTCAAA+GGG	0.528598	4:+59129333	None:intergenic
ATTGGACGAAAACCAGGTAA+AGG	0.530391	4:+59129414	None:intergenic
CATTAAACTCAAAGGGAACA+TGG	0.533197	4:+59129340	None:intergenic
TACCACGCGAAAACAAATAC+AGG	0.534979	4:-59129032	MsaT020833.1:CDS
CGAGACATATAAACAGTGGC+AGG	0.537307	4:-59128929	MsaT020833.1:CDS
CAGGTTAGGAATACAAGAGC+TGG	0.537591	4:-59128910	MsaT020833.1:CDS
GTCAAGAGTTGAGGCTGCGG+AGG	0.544335	4:+59130215	None:intergenic
GAGAATCCGTTTGTGTTGAA+GGG	0.545933	4:-59130152	MsaT020833.1:CDS
CTCCAGTACCATCGCCGACA+AGG	0.546196	4:+59129706	None:intergenic
AATGAAGTTGAGAAATAGCA+GGG	0.546812	4:+59130366	None:intergenic
TTAGATATTCATAACTCTGA+TGG	0.546938	4:-59130506	MsaT020833.1:CDS
CATTCTACTGGTGTTAGTGA+TGG	0.548607	4:-59130347	MsaT020833.1:CDS
GTTCATAAACCAAATCCTGA+TGG	0.550468	4:-59130645	MsaT020833.1:CDS
CTAACCACTTTGGTCAAGAA+GGG	0.550769	4:-59130889	MsaT020833.1:CDS
TCAGAGTTATGAATATCTAA+GGG	0.551258	4:+59130509	None:intergenic
TACCAAAATCAATGATATGG+AGG	0.553791	4:+59129529	None:intergenic
CTCCAAACCTGAAGATCACC+GGG	0.554977	4:-59129446	MsaT020833.1:CDS
TGGTATGGCATTAAACTCAA+AGG	0.555962	4:+59129332	None:intergenic
AGGTAAAGGGAACTCTATCC+CGG	0.560031	4:+59129428	None:intergenic
CCTCCAAACCTGAAGATCAC+CGG	0.561178	4:-59129447	MsaT020833.1:CDS
AAATCAGTGTCTTCGAGGGA+AGG	0.562271	4:+59130683	None:intergenic
GAGGCACGCCTTGTCGGCGA+TGG	0.563864	4:-59129714	MsaT020833.1:CDS
TCTGTCATTTGCAAGCTAAG+TGG	0.564137	4:+59130590	None:intergenic
AAACAGGGGTATCAGGTGAA+AGG	0.568952	4:+59130423	None:intergenic
ATGAACGTTGTAGCTTGTGA+AGG	0.570356	4:-59128973	MsaT020833.1:CDS
CCAGTCAAGAGTTGAGGCTG+CGG	0.571987	4:+59130212	None:intergenic
ATTCGCCATTCTTTGCTACA+CGG	0.575217	4:-59129110	MsaT020833.1:CDS
TGGATGCTTCAAGGTTGGAA+AGG	0.577566	4:-59128787	MsaT020833.1:CDS
TCTTGGTGTTCGGAGAGGAA+TGG	0.578017	4:+59129673	None:intergenic
TACTCCCTTCTTGACCAAAG+TGG	0.580662	4:+59130885	None:intergenic
CTCAGCAGTAGAGATCCTCT+TGG	0.580706	4:+59129656	None:intergenic
GTGATGTGAGTTGATTATGC+TGG	0.583220	4:+59128733	None:intergenic
CATATAACCTTCTATAGGGA+AGG	0.589911	4:+59130721	None:intergenic
CTTTGCTACACGGTTTAGGG+AGG	0.591419	4:-59129100	MsaT020833.1:CDS
ATGGAAGCGCTTGCAATAGT+CGG	0.593097	4:+59129359	None:intergenic
TTACTTTGCCAATGGCCTTG+AGG	0.593344	4:-59129733	MsaT020833.1:CDS
ATTGATTTGGATCAGCCTCA+AGG	0.595193	4:+59130789	None:intergenic
AAACAAATACAGGATGCTGA+TGG	0.598030	4:-59129022	MsaT020833.1:CDS
CAATTTGGATATGGTTAGGG+AGG	0.598257	4:-59130177	MsaT020833.1:CDS
CTCTGTGGTCCATCCCCAGA+GGG	0.598960	4:+59129765	None:intergenic
ACCTTCTATAGGGAAGGTGG+TGG	0.600256	4:+59130727	None:intergenic
TTTCACTTGTCCATTCTGCA+AGG	0.603209	4:+59129959	None:intergenic
ATCTAAGGGGTGTTGATTAG+GGG	0.605109	4:+59130523	None:intergenic
ACCAAAATCAATGATATGGA+GGG	0.610467	4:+59129530	None:intergenic
ATAACCTTCTATAGGGAAGG+TGG	0.616055	4:+59130724	None:intergenic
AAGCTAAGTGGGTCATAGAA+CGG	0.617850	4:+59130602	None:intergenic
ATACAGGATGCTGATGGAGA+GGG	0.620955	4:-59129016	MsaT020833.1:CDS
ACAGGGGTATCAGGTGAAAG+GGG	0.622249	4:+59130425	None:intergenic
ATTGTTTCCCAATAACGCGA+TGG	0.623674	4:+59129312	None:intergenic
GTCTTCCCTCCATTTGATGG+AGG	0.626646	4:+59129924	None:intergenic
GAGTCACCTCCATCAAATGG+AGG	0.628730	4:-59129930	MsaT020833.1:CDS
TCAAAACCAGTCAAGAGTTG+AGG	0.630836	4:+59130206	None:intergenic
AATACAGGATGCTGATGGAG+AGG	0.637418	4:-59129017	MsaT020833.1:CDS
ATTCCTCTCCGAACACCAAG+AGG	0.643154	4:-59129671	MsaT020833.1:CDS
AGTCACCTCCATCAAATGGA+GGG	0.643984	4:-59129929	MsaT020833.1:CDS
CCTCTGTGGTCCATCCCCAG+AGG	0.644825	4:+59129764	None:intergenic
GCAACATTCTTCACCCTCTG+GGG	0.646104	4:-59129778	MsaT020833.1:CDS
TGGTCAAGAAGGGAGTATAG+TGG	0.646620	4:-59130879	MsaT020833.1:CDS
TTATGCTGGCACCCAACATG+TGG	0.647174	4:+59128747	None:intergenic
AAGGTTTAGAGAGAGTTGAG+AGG	0.649221	4:-59128954	MsaT020833.1:CDS
AAGACTCGTCGTAAGAAACA+AGG	0.649427	4:-59129906	MsaT020833.1:CDS
GATCCTCTTGGTGTTCGGAG+AGG	0.659641	4:+59129668	None:intergenic
GATTAACATGATCAGAAACA+GGG	0.660585	4:+59130408	None:intergenic
AGCTATTAGTGCTATTGACG+AGG	0.669842	4:-59130054	MsaT020833.1:CDS
CAATAGCAATAGCAGCGTCG+AGG	0.672364	4:-59130465	MsaT020833.1:CDS
CAGAGTTATGAATATCTAAG+GGG	0.672632	4:+59130510	None:intergenic
TGAATATGATAGCTTCCTTG+AGG	0.674292	4:-59130804	MsaT020833.1:CDS
AGCAAACAGTCCTAGAAATG+CGG	0.677440	4:-59129199	MsaT020833.1:CDS
ATGAAGTTGAGAAATAGCAG+GGG	0.678292	4:+59130367	None:intergenic
AAGTAATGAGCCAACCTCTG+TGG	0.682694	4:+59129750	None:intergenic
GAGGGAAGGATCCGTCGCCG+AGG	0.685761	4:+59130697	None:intergenic
TCTTAAAATCAAGAGCAATG+AGG	0.688807	4:-59129277	MsaT020833.1:CDS
GTGGTGGCTGCAAAACCAAG+TGG	0.689009	4:+59130743	None:intergenic
TTAGTGAAATCACTAGCTTG+AGG	0.692408	4:+59130908	None:intergenic
GGCCCGAGACATATAAACAG+TGG	0.698214	4:-59128933	MsaT020833.1:CDS
CTGATGGAGAGGGAAAGTAT+CGG	0.714910	4:-59129006	MsaT020833.1:CDS
ATTAACATGATCAGAAACAG+GGG	0.716208	4:+59130409	None:intergenic
GAATGGACAAGTGAAAACGG+AGG	0.763230	4:-59129952	MsaT020833.1:CDS
CCTCTGGGGATGGACCACAG+AGG	0.797161	4:-59129764	MsaT020833.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	59128747	59130945	59128747	ID=MsaG020833
Chr4	mRNA	59128747	59130945	59128747	ID=MsaT020833.1;Parent=MsaG020833
Chr4	exon	59128747	59130945	59128747	ID=MsaT020833.1.exon1;Parent=MsaT020833.1
Chr4	CDS	59128747	59130945	59128747	ID=cds.MsaT020833.1;Parent=MsaT020833.1

Gene Sequence

>MsaT020833.1

ATGGATCCAAACTTTCCTCAAGCTAGTGATTTCACTAACCACTTTGGTCAAGAAGGGAGTATAGTGGTCTCTGAGTTTGATCCAAATCAATATGATAGCTACCTTGATGCTGATCCAAATGAATATGATAGCTTCCTTGAGGCTGATCCAAATCAATATGATAGCTTTCTTGGTGCTGATCCACTTGGTTTTGCAGCCACCACCTTCCCTATAGAAGGTTATATGGCCTCGGCGACGGATCCTTCCCTCGAAGACACTGATTTTTCAGATACTGGTAAGTTCATAAACCAAATCCTGATGGAAGAAAATGTTGATCAGAGACCGTTCTATGACCCACTTAGCTTGCAAATGACAGAAAAATCATTCTATGATGCTCTCTTACACACAAATAAACCTCTTTCCCCTAATCAACACCCCTTAGATATTCATAACTCTGATGGCACAACCAGCAGCAACAGCAATAGCAATAGCAGCGTCGAGGATTCTCGAGAGTTAAAACCCCTTTCACCTGATACCCCTGTTTCTGATCATGTTAATCATGTTTTTCAGTTTAATTCCCCTGCTATTTCTCAACTTCATTCTACTGGTGTTAGTGATGGACTCAGAGATTTGGATTCTTCTATTACACAGATGGCACAAAACATTTTCAGTGATGTAGATTCTGTTTCCCTATTCAAGAAAAGTTTAGAAGAAGGCAACAAGTTTCTTCCTCCGCAGCCTCAACTCTTGACTGGTTTTGAATCATCCAATTTGGATATGGTTAGGGAGGAAGAGAATCCGTTTGTGTTGAAGGGTAGAAAGAATCACAAGCGCGAAGAAAGTTTTAGTCAAGAACAAGAAGAAGAAGCTTGGAGAAGTAGCAAGCAATCAGCTATTAGTGCTATTGACGAGGATGAATTATCAGAGATGTTTGATAAGGTTTTGCTTGATGTGGGACATTTGCCATTATGCAATGAAAATGATTCCTTGCAGAATGGACAAGTGAAAACGGAGGAGTCACCTCCATCAAATGGAGGGAAGACTCGTCGTAAGAAACAAGGCAAGAAGAATGAAACAATAGATTTGAGGAATCTTCTGCTTATGTGTTCACAATCTGTATATGCCAATGACAACAGGAATGCTAATGAGCTACTGAAGCAGATTAGGCAACATTCTTCACCCTCTGGGGATGGACCACAGAGGTTGGCTCATTACTTTGCCAATGGCCTTGAGGCACGCCTTGTCGGCGATGGTACTGGAGCACAAACATTCCATTCCTCTCCGAACACCAAGAGGATCTCTACTGCTGAGTTTTTGAAAGCATATCAAGTTCATCTATCTACTAGTCCTTTCAAAAAGTTTGCATACTTCTTTGCAAATAAGATGATTATGAAAGCTTCTGCAAATGCTGAAACCCTCCATATCATTGATTTTGGTATCCTATATGGTTTTCAGTGGCCAATACTTATAAAGTTTTTATCAGATAGAGAAGGTGGACCTCCAAACCTGAAGATCACCGGGATAGAGTTCCCTTTACCTGGTTTTCGTCCAATGGAAAGAATCGAAGAAACTGGTCGTCGTCTTGCCGACTATTGCAAGCGCTTCCATGTTCCCTTTGAGTTTAATGCCATACCATCGCGTTATTGGGAAACAATTCGAGTTGAGGATCTTAAAATCAAGAGCAATGAGGTTGTTGTTGTGAACTCTTTGATGAGATTTAAGAATTTATTGGATGAAAGTATTGAAGCAAACAGTCCTAGAAATGCGGTTCTGCATTTAATCAGAAAGATAAATCCTGCTATTTTTGTTCAGTCAATTGTTAATGGATCATATAATTCGCCATTCTTTGCTACACGGTTTAGGGAGGCGCTGTTTCATTTTTCTGCTCTTTATGATATGTTTGACACTGTCATACCACGCGAAAACAAATACAGGATGCTGATGGAGAGGGAAAGTATCGGTAGAGAAGCTATGAACGTTGTAGCTTGTGAAGGTTTAGAGAGAGTTGAGAGGCCCGAGACATATAAACAGTGGCAGGTTAGGAATACAAGAGCTGGTTTCAAACAGCTTCCATTGAATTCGGAATTAATGGACAAATTTAGGACCAAGTTACAGCAATGTTACCACAAAGACTTTGTTTTTGATGTAGACAACAATTGGATGCTTCAAGGTTGGAAAGGACGCATTTTATATGCTTCCACATGTTGGGTGCCAGCATAA

Protein sequence

>MsaT020833.1

MDPNFPQASDFTNHFGQEGSIVVSEFDPNQYDSYLDADPNEYDSFLEADPNQYDSFLGADPLGFAATTFPIEGYMASATDPSLEDTDFSDTGKFINQILMEENVDQRPFYDPLSLQMTEKSFYDALLHTNKPLSPNQHPLDIHNSDGTTSSNSNSNSSVEDSRELKPLSPDTPVSDHVNHVFQFNSPAISQLHSTGVSDGLRDLDSSITQMAQNIFSDVDSVSLFKKSLEEGNKFLPPQPQLLTGFESSNLDMVREEENPFVLKGRKNHKREESFSQEQEEEAWRSSKQSAISAIDEDELSEMFDKVLLDVGHLPLCNENDSLQNGQVKTEESPPSNGGKTRRKKQGKKNETIDLRNLLLMCSQSVYANDNRNANELLKQIRQHSSPSGDGPQRLAHYFANGLEARLVGDGTGAQTFHSSPNTKRISTAEFLKAYQVHLSTSPFKKFAYFFANKMIMKASANAETLHIIDFGILYGFQWPILIKFLSDREGGPPNLKITGIEFPLPGFRPMERIEETGRRLADYCKRFHVPFEFNAIPSRYWETIRVEDLKIKSNEVVVVNSLMRFKNLLDESIEANSPRNAVLHLIRKINPAIFVQSIVNGSYNSPFFATRFREALFHFSALYDMFDTVIPRENKYRMLMERESIGREAMNVVACEGLERVERPETYKQWQVRNTRAGFKQLPLNSELMDKFRTKLQQCYHKDFVFDVDNNWMLQGWKGRILYASTCWVPA*