AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020863


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020863 MtrunA17_Chr4g0032111 93.258 178 7 2 1 178 1 173 1.41e-117 330
MsaG020863 MtrunA17_Chr7g0239441 37.107 159 57 5 20 178 36 151 5.27e-20 81.6
MsaG020863 MtrunA17_Chr1g0187231 60.317 63 21 2 10 69 8 69 2.34e-16 73.2
MsaG020863 MtrunA17_Chr1g0168181 44.304 79 43 1 13 90 18 96 1.56e-14 67.8
MsaG020863 MtrunA17_Chr1g0184331 70.732 41 11 1 21 60 38 78 7.65e-14 65.5
MsaG020863 MtrunA17_Chr3g0112181 65.909 44 13 1 21 64 55 96 2.17e-13 66.2
MsaG020863 MtrunA17_Chr4g0009461 74.286 35 9 0 21 55 59 93 2.90e-13 66.2
MsaG020863 MtrunA17_Chr2g0315591 30.460 174 85 6 21 162 50 219 3.52e-13 65.1
MsaG020863 MtrunA17_Chr5g0400571 32.768 177 79 6 21 165 80 248 3.77e-13 65.5
MsaG020863 MtrunA17_Chr5g0435711 72.973 37 10 0 21 57 47 83 5.50e-13 64.7
MsaG020863 MtrunA17_Chr1g0184381 66.667 33 11 0 21 53 35 67 1.51e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020863 AT3G53820.1 35.220 159 63 7 21 174 19 142 2.03e-17 75.1
MsaG020863 AT3G09290.1 80.000 35 7 0 21 55 33 67 1.27e-15 71.2
MsaG020863 AT5G06070.1 72.222 36 10 0 21 56 53 88 2.46e-13 65.9
MsaG020863 AT5G43540.1 55.357 56 17 2 23 70 35 90 8.88e-13 62.8
MsaG020863 AT2G37740.1 59.091 44 18 0 11 54 27 70 1.65e-12 64.7
MsaG020863 AT3G23130.1 68.571 35 11 0 21 55 45 79 2.62e-12 62.8
MsaG020863 AT4G17810.2 32.414 145 83 4 21 165 44 173 6.05e-12 61.2
MsaG020863 AT2G42410.3 56.098 41 18 0 21 61 73 113 2.52e-11 60.8
MsaG020863 AT2G42410.2 56.098 41 18 0 21 61 47 87 2.54e-11 60.5
MsaG020863 AT2G42410.1 56.098 41 18 0 21 61 47 87 2.54e-11 60.5
MsaG020863 AT4G17810.1 64.103 39 13 1 21 58 44 82 2.80e-11 60.1

Find 33 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCAATTACCATCTAATT+TGG 0.170331 4:-59520188 MsaT020863.1:CDS
CTGGTACCCGTTGGCTTTGA+TGG 0.228413 4:+59519869 None:intergenic
AATTAGATGGTAATTGAGTT+TGG 0.335367 4:+59520191 None:intergenic
TTAGACCTTGAACTAAGATT+AGG 0.355982 4:-59519908 MsaT020863.1:CDS
ACAATATTAGTATTCAAGTT+TGG 0.372182 4:+59520130 None:intergenic
TAGACCTTGAACTAAGATTA+GGG 0.384535 4:-59519907 MsaT020863.1:CDS
AATTACACTCATAAGATCTA+AGG 0.395050 4:+59520314 None:intergenic
CCCTTTGAACTTGACCTTGT+GGG 0.399837 4:+59519987 None:intergenic
GCTTTGCTTTGTCTTTCCTA+TGG 0.427666 4:+59520239 None:intergenic
GGAAATTATTACTACACAAA+AGG 0.438306 4:-59519966 MsaT020863.1:CDS
GAGTCTGATCAACATGATAA+AGG 0.445854 4:-59520367 MsaT020863.1:CDS
GAACTTCCCTCCAAATTAGA+TGG 0.446674 4:+59520178 None:intergenic
ATTTGTGTTTCATTTCTAGT+TGG 0.455559 4:+59520016 None:intergenic
TTCTCTAATGCACAAGCTCT+TGG 0.469634 4:-59520277 MsaT020863.1:CDS
AGAATTTCATCACCTCTAGA+AGG 0.475968 4:+59520082 None:intergenic
TCCCACAAGGTCAAGTTCAA+AGG 0.482764 4:-59519988 MsaT020863.1:CDS
TCAATTACCATCTAATTTGG+AGG 0.483513 4:-59520185 MsaT020863.1:CDS
CACCTCTAGAAGGAGCTTGT+TGG 0.486575 4:+59520092 None:intergenic
CCCACAAGGTCAAGTTCAAA+GGG 0.499228 4:-59519987 MsaT020863.1:CDS
TTTGATGGATCATGTGGCTC+AGG 0.503162 4:+59519884 None:intergenic
CAGGCCCTAATCTTAGTTCA+AGG 0.522982 4:+59519903 None:intergenic
CATGATCCATCAAAGCCAAC+GGG 0.534522 4:-59519875 MsaT020863.1:CDS
TCTAATGCACAAGCTCTTGG+TGG 0.538110 4:-59520274 MsaT020863.1:CDS
TCCCTTTGAACTTGACCTTG+TGG 0.538332 4:+59519986 None:intergenic
TCCGATGAAGAATCTTGAGC+AGG 0.539565 4:+59519932 None:intergenic
ACCTGCTCAAGATTCTTCAT+CGG 0.568940 4:-59519933 MsaT020863.1:CDS
CAATTACCATCTAATTTGGA+GGG 0.604261 4:-59520184 MsaT020863.1:CDS
GTGGACACATGAATATCCAT+AGG 0.606044 4:-59520255 MsaT020863.1:CDS
CACCAACAAGCTCCTTCTAG+AGG 0.609713 4:-59520094 MsaT020863.1:CDS
ACATGATCCATCAAAGCCAA+CGG 0.613032 4:-59519876 MsaT020863.1:CDS
GTTGGCTTTGATGGATCATG+TGG 0.637580 4:+59519878 None:intergenic
TCTGATCAACATGATAAAGG+TGG 0.671643 4:-59520364 MsaT020863.1:CDS
ACACAAATTAATATCCCACA+AGG 0.716304 4:-59520001 MsaT020863.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 59519856 59520392 59519856 ID=MsaG020863
Chr4 mRNA 59519856 59520392 59519856 ID=MsaT020863.1;Parent=MsaG020863
Chr4 exon 59519856 59520392 59519856 ID=MsaT020863.1.exon1;Parent=MsaT020863.1
Chr4 CDS 59519856 59520392 59519856 ID=cds.MsaT020863.1;Parent=MsaT020863.1
Gene Sequence

>MsaT020863.1

ATGGAGTCTGATCAACATGATAAAGGTGGTGATGATCAAGATGCAAAACAAGTGAACCTTAGATCTTATGAGTGTAATTTTTGCAAGAGAGGATTCTCTAATGCACAAGCTCTTGGTGGACACATGAATATCCATAGGAAAGACAAAGCAAAGCTCAAACAACAATCTTCAACAAATTACCAAACTCAATTACCATCTAATTTGGAGGGAAGTTCTAGTGAAGAGATCAACAAATTATTACCAAACTTGAATACTAATATTGTGTCTCAACAAGATCACCAACAAGCTCCTTCTAGAGGTGATGAAATTCTTGTAACTCAAGTGAGAACACAACTTCCACTTTTTGCTGAATCACCAACTAGAAATGAAACACAAATTAATATCCCACAAGGTCAAGTTCAAAGGGAAATTATTACTACACAAAAGGCTTTGTTATTACCTGCTCAAGATTCTTCATCGGAGTTAGACCTTGAACTAAGATTAGGGCCTGAGCCACATGATCCATCAAAGCCAACGGGTACCAGAAAATTCTTTTAA

Protein sequence

>MsaT020863.1

MESDQHDKGGDDQDAKQVNLRSYECNFCKRGFSNAQALGGHMNIHRKDKAKLKQQSSTNYQTQLPSNLEGSSSEEINKLLPNLNTNIVSQQDHQQAPSRGDEILVTQVRTQLPLFAESPTRNETQINIPQGQVQREIITTQKALLLPAQDSSSELDLELRLGPEPHDPSKPTGTRKFF*