AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020918


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020918 MtrunA17_Chr4g0032951 89.116 441 21 5 1 422 1 433 0.0 734
MsaG020918 MtrunA17_Chr2g0329041 50.327 459 141 17 1 422 1 409 2.75e-129 379
MsaG020918 MtrunA17_Chr1g0174271 44.015 259 115 8 181 420 127 374 8.17e-56 189
MsaG020918 MtrunA17_Chr3g0087771 32.151 451 193 17 15 422 21 401 3.51e-40 148
MsaG020918 MtrunA17_Chr7g0258181 35.249 261 129 5 174 420 85 319 3.96e-35 132
MsaG020918 MtrunA17_Chr8g0351881 32.955 264 122 10 181 417 154 389 1.74e-25 107
MsaG020918 MtrunA17_Chr4g0014771 54.545 88 27 2 341 417 106 191 2.15e-23 97.1
MsaG020918 MtrunA17_Chr1g0213791 48.598 107 43 2 325 420 236 341 1.44e-21 95.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020918 AT1G51140.1 36.406 434 202 17 1 417 1 377 1.67e-59 199
MsaG020918 AT2G42280.1 31.796 412 198 14 25 422 15 357 1.61e-43 156
MsaG020918 AT2G42280.3 31.313 396 189 15 25 406 15 341 5.80e-36 135
MsaG020918 AT2G43140.1 50.962 104 39 2 325 416 103 206 1.55e-25 103
MsaG020918 AT2G43140.2 50.962 104 39 2 325 416 200 303 6.73e-25 104
MsaG020918 AT1G35460.1 51.724 87 31 1 347 422 173 259 2.06e-23 99.0
MsaG020918 AT4G09180.1 37.654 162 93 3 265 422 105 262 1.45e-22 96.7
MsaG020918 AT1G05805.1 48.077 104 41 3 325 416 253 355 2.40e-21 95.1

Find 79 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAATTCGGCCGTAGTATT+TGG 0.246472 4:-60370631 None:intergenic
CATCAGCAACAAATGAATTC+AGG 0.254682 4:+60368706 MsaT020918.1:CDS
AGTAATAATATGAGTAATTA+TGG 0.267441 4:+60369093 MsaT020918.1:CDS
CCAGACTACTTCTGCTCCTT+TGG 0.303630 4:+60370591 MsaT020918.1:CDS
TCAACTACTGAATCAGTTAT+AGG 0.313043 4:-60368931 None:intergenic
ATCTTTACCTTATCCATGTT+TGG 0.314679 4:-60371305 None:intergenic
CTTCTCAATGGCTGCCAATT+CGG 0.320940 4:-60370644 None:intergenic
CGGGTTTCAGCAAAACCTTC+AGG 0.325691 4:-60370151 None:intergenic
GCCAAATACTACGGCCGAAT+TGG 0.337775 4:+60370630 MsaT020918.1:CDS
CCTAACGGTGAGGCATAGTT+TGG 0.353263 4:-60370085 None:intergenic
CTTTCTGTTTCAGGACTTGA+AGG 0.354030 4:-60368811 None:intergenic
TTTGCTGCTATGAGAGGAAT+AGG 0.354357 4:+60369989 MsaT020918.1:CDS
TATGAGAGGAATAGGAACTA+TGG 0.359228 4:+60369997 MsaT020918.1:CDS
GGTGGTAGTGGTGGTGGTGG+TGG 0.363507 4:+60368871 MsaT020918.1:CDS
GGATTTAAACCTGGAGGAAA+TGG 0.364210 4:-60370286 None:intergenic
TCAAACATTAACATTGATAC+TGG 0.367647 4:+60369234 MsaT020918.1:CDS
ATGATATCTGACAATATTAC+TGG 0.368731 4:+60370229 MsaT020918.1:CDS
TCAACCTCTTCCTTAACTAT+AGG 0.381573 4:-60368997 None:intergenic
TTTATCCAGGGTTTCCCCTT+AGG 0.387747 4:+60370184 MsaT020918.1:CDS
TCACACAAGCAACAGCAATA+AGG 0.392323 4:+60371809 MsaT020918.1:CDS
GGAAGTGGTGGTAGTGGTGG+TGG 0.399180 4:+60368865 MsaT020918.1:CDS
CGACCGGTCTTCATTTGATT+CGG 0.402592 4:-60369168 None:intergenic
TTTAAATCCAGCTGAAACTA+AGG 0.405121 4:+60370300 MsaT020918.1:CDS
CACACAAGCAACAGCAATAA+GGG 0.405182 4:+60371810 MsaT020918.1:CDS
TCACCGTTAGGAGCTGAAAT+CGG 0.415027 4:+60370097 MsaT020918.1:CDS
CCAAAGGAGCAGAAGTAGTC+TGG 0.416991 4:-60370591 None:intergenic
GATTCTGTTCCGTGCAAAAC+TGG 0.418157 4:+60370682 MsaT020918.1:CDS
TCAACTACCTTAGTTTCAGC+TGG 0.426767 4:-60370307 None:intergenic
TGCATTGATCAGAATGAAAC+AGG 0.428524 4:+60370565 MsaT020918.1:intron
GCAACAGCTTCCTATAGTTA+AGG 0.446191 4:+60368987 MsaT020918.1:CDS
AGTGGTGGTAGTGGTGGTGG+TGG 0.447971 4:+60368868 MsaT020918.1:CDS
CACCGTTAGGAGCTGAAATC+GGG 0.453811 4:+60370098 MsaT020918.1:CDS
ATCCTCCCATGTAGTACCTA+AGG 0.461462 4:-60370200 None:intergenic
TGATGATCTAGAAACATAGT+TGG 0.464492 4:-60368676 None:intergenic
CCAAACTATGCCTCACCGTT+AGG 0.468050 4:+60370085 MsaT020918.1:CDS
ATGAATAGTCTTAGTGGAAG+TGG 0.476450 4:+60368850 MsaT020918.1:CDS
AAGGTGGTCTTCCTGAACTT+TGG 0.484772 4:-60369143 None:intergenic
ATCAGCAACAAATGAATTCA+GGG 0.496532 4:+60368707 MsaT020918.1:CDS
CTAAAATTAGCGAGCGAATG+AGG 0.505851 4:+60371264 MsaT020918.1:CDS
AGTGGAAGTGGTGGTAGTGG+TGG 0.511352 4:+60368862 MsaT020918.1:CDS
GTTTCCCCTTAGGTACTACA+TGG 0.511883 4:+60370194 MsaT020918.1:CDS
GTTTCAGCTGGATTTAAACC+TGG 0.516134 4:-60370295 None:intergenic
CTTAGTGGAAGTGGTGGTAG+TGG 0.518560 4:+60368859 MsaT020918.1:CDS
TTCATTTGATTCGGCAAAGG+TGG 0.519821 4:-60369159 None:intergenic
AGGTTTATGAATAGTCTTAG+TGG 0.525192 4:+60368844 MsaT020918.1:CDS
TCTCGTGCCAAACATGGATA+AGG 0.525796 4:+60371298 MsaT020918.1:CDS
CACATCAGACATGTTGGACT+TGG 0.527533 4:+60371425 MsaT020918.1:CDS
GATGTAAAACCATTTCCTCC+AGG 0.529045 4:+60370277 MsaT020918.1:CDS
AGACATGGCAGTTCACCTGC+TGG 0.529643 4:+60369204 MsaT020918.1:CDS
GTCTTCATTTGATTCGGCAA+AGG 0.532649 4:-60369162 None:intergenic
GCTTCCTATAGTTAAGGAAG+AGG 0.542479 4:+60368993 MsaT020918.1:CDS
CGAGCGAATGAGGAAACTAC+AGG 0.543755 4:+60371274 MsaT020918.1:CDS
AACAAACACATCAGACATGT+TGG 0.549768 4:+60371419 MsaT020918.1:CDS
ATGAGAGGAATAGGAACTAT+GGG 0.549779 4:+60369998 MsaT020918.1:CDS
ATGTTTGAAAACAATCCAGC+AGG 0.553542 4:-60369219 None:intergenic
CTCCCGATTTCAGCTCCTAA+CGG 0.555564 4:-60370100 None:intergenic
CTGGATAAAATCGTTACCCC+GGG 0.557487 4:-60370170 None:intergenic
TCCTCCCATGTAGTACCTAA+GGG 0.558794 4:-60370199 None:intergenic
CAAAACAATCTTGAACCTGA+AGG 0.566426 4:+60370136 MsaT020918.1:CDS
CGGTCTAGTCTTATTAGACA+TGG 0.571581 4:+60369189 MsaT020918.1:CDS
ACAGGATCTCGTGCCAAACA+TGG 0.576148 4:+60371292 MsaT020918.1:CDS
CCTGGATAAAATCGTTACCC+CGG 0.588424 4:-60370171 None:intergenic
AAATGAATTCAGGGTTAACA+CGG 0.591208 4:+60368716 MsaT020918.1:CDS
AATGAATAACAATGAATCTG+TGG 0.596436 4:+60369044 MsaT020918.1:CDS
CCCCTTAGGTACTACATGGG+AGG 0.597480 4:+60370198 MsaT020918.1:CDS
GATGAGTATGCCAAATACTA+CGG 0.608721 4:+60370621 MsaT020918.1:CDS
ATACTCATCTGATGAGCCAA+AGG 0.612846 4:-60370607 None:intergenic
TCTAATAAGACTAGACCGAC+CGG 0.616887 4:-60369184 None:intergenic
TAGTACCTAAGGGGAAACCC+TGG 0.618141 4:-60370189 None:intergenic
TTGCCGAATCAAATGAAGAC+CGG 0.622612 4:+60369165 MsaT020918.1:CDS
AATAGTCTTAGTGGAAGTGG+TGG 0.627049 4:+60368853 MsaT020918.1:CDS
TCAGCTGGATTTAAACCTGG+AGG 0.642939 4:-60370292 None:intergenic
CGAATCAAATGAAGACCGGT+CGG 0.663039 4:+60369169 MsaT020918.1:CDS
CCTCCCATGTAGTACCTAAG+GGG 0.665348 4:-60370198 None:intergenic
AGACCTACAAAATCAAGTTG+AGG 0.675547 4:+60371464 MsaT020918.1:CDS
TTTCCCCTTAGGTACTACAT+GGG 0.680721 4:+60370195 MsaT020918.1:CDS
ATTGATCAGAATGAAACAGG+AGG 0.687350 4:+60370568 MsaT020918.1:intron
GATTTCAGCTCCTAACGGTG+AGG 0.706801 4:-60370095 None:intergenic
GTGCAAAACTGGTATAAACG+AGG 0.724057 4:+60370693 MsaT020918.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 60368652 60371829 60368652 ID=MsaG020918
Chr4 mRNA 60368652 60371829 60368652 ID=MsaT020918.1;Parent=MsaG020918
Chr4 exon 60368652 60369255 60368652 ID=MsaT020918.1.exon1;Parent=MsaT020918.1
Chr4 CDS 60368652 60369255 60368652 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Chr4 exon 60369987 60370321 60369987 ID=MsaT020918.1.exon2;Parent=MsaT020918.1
Chr4 CDS 60369987 60370321 60369987 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Chr4 exon 60370577 60370717 60370577 ID=MsaT020918.1.exon3;Parent=MsaT020918.1
Chr4 CDS 60370577 60370717 60370577 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Chr4 exon 60371254 60371319 60371254 ID=MsaT020918.1.exon4;Parent=MsaT020918.1
Chr4 CDS 60371254 60371319 60371254 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Chr4 exon 60371417 60371485 60371417 ID=MsaT020918.1.exon5;Parent=MsaT020918.1
Chr4 CDS 60371417 60371485 60371417 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Chr4 exon 60371776 60371829 60371776 ID=MsaT020918.1.exon6;Parent=MsaT020918.1
Chr4 CDS 60371776 60371829 60371776 ID=cds.MsaT020918.1;Parent=MsaT020918.1
Gene Sequence

>MsaT020918.1

ATGGAGTCAGATCTTCAGCAGCATCCAACTATGTTTCTAGATCATCAAAATCATCATCAGCAACAAATGAATTCAGGGTTAACACGGTTTAAATCTGCTCCAAGTTCATATTTTTCAAATATCATTGATAAAGAGTTTTATGAGCATCTTTTCAATAAACCTTCAAGTCCTGAAACAGAAAGAGTTTTTGCAAGGTTTATGAATAGTCTTAGTGGAAGTGGTGGTAGTGGTGGTGGTGGTGGTGGTGCTGAAAGTGTTTCTGTTACAGCTTCTATAGCTCCTATAACTGATTCAGTAGTTGATTCATTGACTCAGAATCTATCAACTGTTCAACAGCAACAGCTTCCTATAGTTAAGGAAGAGGTTGATCAACAATCTCAAACTATGCAAACAATGAATAACAATGAATCTGTGGAACTTCAACAATTTCAAAGACAACAAAGTAATAATATGAGTAATTATGGTTCTTCTGTTGCTCCTCAGAATTTTTACCAAAGTTCAGGAAGACCACCTTTGCCGAATCAAATGAAGACCGGTCGGTCTAGTCTTATTAGACATGGCAGTTCACCTGCTGGATTGTTTTCAAACATTAACATTGATACTGGTTTTGCTGCTATGAGAGGAATAGGAACTATGGGAGCTGCTAATAGCGCTAGCAAAGAAGCAAATTTTTCTTCTTCTGCGGTACGGTTAAAGAATGCTCCAAACTATGCCTCACCGTTAGGAGCTGAAATCGGGAGTAATAGCATTCCACAAAACAATCTTGAACCTGAAGGTTTTGCTGAAACCCGGGGTAACGATTTTATCCAGGGTTTCCCCTTAGGTACTACATGGGAGGATACTGCGATGATATCTGACAATATTACTGGCCTAAAAAGATACAGAGATGATGATGATGTAAAACCATTTCCTCCAGGTTTAAATCCAGCTGAAACTAAGAATGAAACAGGAGGCCAGACTACTTCTGCTCCTTTGGCTCATCAGATGAGTATGCCAAATACTACGGCCGAATTGGCAGCCATTGAGAAGTTTTTGCAGTTCTCAGATTCTGTTCCGTGCAAAACTGGTATAAACGAGGCTGTTAGAAGAACTAAAATTAGCGAGCGAATGAGGAAACTACAGGATCTCGTGCCAAACATGGATAAGCAAACAAACACATCAGACATGTTGGACTTGGCTGTTGAGTACATTAAAGACCTACAAAATCAAGTTGAGACTCTTTCAGACAATCGAGCTAAGTGCACGTGTTCACACAAGCAACAGCAATAA

Protein sequence

>MsaT020918.1

MESDLQQHPTMFLDHQNHHQQQMNSGLTRFKSAPSSYFSNIIDKEFYEHLFNKPSSPETERVFARFMNSLSGSGGSGGGGGGAESVSVTASIAPITDSVVDSLTQNLSTVQQQQLPIVKEEVDQQSQTMQTMNNNESVELQQFQRQQSNNMSNYGSSVAPQNFYQSSGRPPLPNQMKTGRSSLIRHGSSPAGLFSNINIDTGFAAMRGIGTMGAANSASKEANFSSSAVRLKNAPNYASPLGAEIGSNSIPQNNLEPEGFAETRGNDFIQGFPLGTTWEDTAMISDNITGLKRYRDDDDVKPFPPGLNPAETKNETGGQTTSAPLAHQMSMPNTTAELAAIEKFLQFSDSVPCKTGINEAVRRTKISERMRKLQDLVPNMDKQTNTSDMLDLAVEYIKDLQNQVETLSDNRAKCTCSHKQQQ*