AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020965


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020965 MtrunA17_Chr4g0033581 85.098 510 61 4 1 500 1 505 0.0 824
MsaG020965 MtrunA17_Chr8g0383101 52.000 400 155 10 131 500 371 763 2.80e-79 263
MsaG020965 MtrunA17_Chr8g0383111 59.259 108 42 2 127 233 53 159 8.35e-17 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020965 AT4G39160.1 35.741 263 140 9 185 436 277 521 2.15e-29 122
MsaG020965 AT4G39160.3 35.741 263 140 9 185 436 277 521 2.15e-29 122
MsaG020965 AT4G39160.2 31.268 355 175 14 185 500 277 601 2.72e-24 107

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAACATAACTTACCATTAT+TGG 0.143575 4:-61352323 None:intergenic
GTTCTCAATCAATTTCTCTT+AGG 0.163126 4:+61353382 MsaT020965.1:CDS
AAACATAACTTACCATTATT+GGG 0.214638 4:-61352322 None:intergenic
GAGAAGAATGAGAGAACTTT+TGG 0.248085 4:-61353223 None:intergenic
TCTTGCAATTGCAGAAAATA+AGG 0.274714 4:+61353410 MsaT020965.1:CDS
TCTATATTGTTACAAATATA+AGG 0.289417 4:-61353043 None:intergenic
ACCATTATTGGGGTTGGTCT+CGG 0.333847 4:-61352311 None:intergenic
CACAGTGGACCAGGCTCTGC+TGG 0.334784 4:+61353344 MsaT020965.1:CDS
GTTGAATCATTGTAAAATCT+AGG 0.346657 4:-61354299 None:intergenic
CCATTATTGGGGTTGGTCTC+GGG 0.353675 4:-61352310 None:intergenic
ACAAGTCACCTAGGGTAAAA+TGG 0.357410 4:+61354086 MsaT020965.1:CDS
CATGCTACCTCGTCTGTAGA+TGG 0.366233 4:+61352241 MsaT020965.1:CDS
CTGCTGGATGATTATGAACT+TGG 0.367304 4:+61353360 MsaT020965.1:CDS
GGCTTCCTGATTTGCATTAG+TGG 0.370648 4:-61354610 None:intergenic
CACTTCTGAAGCCAACATTT+AGG 0.375419 4:-61352567 None:intergenic
CCCGAGACCAACCCCAATAA+TGG 0.380871 4:+61352310 MsaT020965.1:CDS
TTCTACCACTAATGCAAATC+AGG 0.389275 4:+61354605 MsaT020965.1:CDS
TTCTCAATCAATTTCTCTTA+GGG 0.392485 4:+61353383 MsaT020965.1:CDS
TACTAGCTTGATCATACTCT+TGG 0.398834 4:-61354012 None:intergenic
GTTGGCTTCAGAAGTGCTTC+AGG 0.403637 4:+61352574 MsaT020965.1:CDS
GGGGATTTCTTTCATGATGC+TGG 0.405910 4:+61353938 MsaT020965.1:CDS
AAAAGTTCTCTCATTCTTCT+CGG 0.409991 4:+61353225 MsaT020965.1:CDS
ACATAGGCTCTGAAGGAGTT+TGG 0.412678 4:+61354276 MsaT020965.1:intron
GCTATTAATAGTCGTTCATC+AGG 0.426472 4:+61354405 MsaT020965.1:CDS
CGTAAAAGATCATCTGTTGC+TGG 0.432583 4:+61352911 MsaT020965.1:CDS
ATTGGGGTTGGTCTCGGGAA+TGG 0.446564 4:-61352305 None:intergenic
AAACTCAAACTCATTAATTG+AGG 0.471340 4:+61353000 MsaT020965.1:CDS
GTGATGATGACTTACGGAAA+TGG 0.472624 4:+61355255 MsaT020965.1:CDS
AGAGTGCATACATTAATTCC+AGG 0.475172 4:-61354648 None:intergenic
TTTCATTCCCACAGTGGACC+AGG 0.476047 4:+61353335 MsaT020965.1:CDS
CTGTGTTGCTTACCTTCGAT+TGG 0.477352 4:-61353571 None:intergenic
CAGTGCTGATGTTAAAGATC+AGG 0.478050 4:+61355190 MsaT020965.1:CDS
TGCAGATATTTCCTTTCGTA+AGG 0.479200 4:-61352212 None:intergenic
AATGGAATGAATGTTGTTCT+CGG 0.482678 4:-61352617 None:intergenic
TAACTTACCATTATTGGGGT+TGG 0.488823 4:-61352317 None:intergenic
CATTACTAACCACGTGCAGC+AGG 0.498380 4:-61352047 None:intergenic
GCATTAGTGGTAGAAATTTG+TGG 0.499145 4:-61354597 None:intergenic
TGTTGCTTACCTTCGATTGG+TGG 0.499150 4:-61353568 None:intergenic
TCACTATCATCAGATTGCTC+TGG 0.502518 4:-61355236 None:intergenic
TGCAGAAAATAAGGAGCGAC+CGG 0.506681 4:+61353419 MsaT020965.1:CDS
TCATTAATTGAGGATGATGA+TGG 0.509072 4:+61353010 MsaT020965.1:CDS
ATAGCATCAGTTATTCGTAA+AGG 0.518506 4:-61354387 None:intergenic
CATAATCATCCAGCAGAGCC+TGG 0.529086 4:-61353353 None:intergenic
AACATAACTTACCATTATTG+GGG 0.531955 4:-61352321 None:intergenic
CTCTGGACTATGATAAGCCA+TGG 0.534113 4:-61355219 None:intergenic
GCCACTGAAGACATGTCACC+TGG 0.534938 4:+61354630 MsaT020965.1:CDS
GGAATGGATTTAGTGACGGT+GGG 0.535901 4:-61352289 None:intergenic
AATGGATTTAGTGACGGTGG+GGG 0.543456 4:-61352287 None:intergenic
CTGTCAAGGAATGCAGTGGA+TGG 0.545069 4:-61352505 None:intergenic
AAGATCATCTGTTGCTGGTG+TGG 0.557955 4:+61352916 MsaT020965.1:CDS
ATGCTACCTCGTCTGTAGAT+GGG 0.560684 4:+61352242 MsaT020965.1:CDS
AATATTTAGCTGCAGTGGTG+GGG 0.567750 4:+61353919 MsaT020965.1:intron
GTCAAGGAATGCAGTGGATG+GGG 0.578734 4:-61352503 None:intergenic
ATGATAGTGATGATGACTTA+CGG 0.580140 4:+61355249 MsaT020965.1:CDS
TTGCAAGACAGCCTAAATGT+TGG 0.580193 4:+61352556 MsaT020965.1:CDS
TTTATTAGGATGCTAATGAG+AGG 0.585716 4:+61352870 MsaT020965.1:intron
ATCAAAACAGTCCTTACGAA+AGG 0.590154 4:+61352201 MsaT020965.1:CDS
AGCAGAGCCTGGTCCACTGT+GGG 0.591314 4:-61353342 None:intergenic
GAATGGATTTAGTGACGGTG+GGG 0.591826 4:-61352288 None:intergenic
CTCGGGAATGGATTTAGTGA+CGG 0.594357 4:-61352293 None:intergenic
AAATGATGACGAGGACGAAG+TGG 0.594931 4:+61353078 MsaT020965.1:CDS
TCCAGGTGACATGTCTTCAG+TGG 0.596510 4:-61354631 None:intergenic
TGCTGATGTTAAAGATCAGG+AGG 0.606209 4:+61355193 MsaT020965.1:CDS
AGAATGTGAATTGTATAAAG+CGG 0.606408 4:-61354056 None:intergenic
CAAAATCTTCCACCAATCGA+AGG 0.613148 4:+61353559 MsaT020965.1:CDS
GGGAATGGATTTAGTGACGG+TGG 0.613872 4:-61352290 None:intergenic
CTTGAAGCTCCTGCTGCACG+TGG 0.616756 4:+61352038 MsaT020965.1:CDS
TGTCAAGGAATGCAGTGGAT+GGG 0.621373 4:-61352504 None:intergenic
TAAAGATCAGGAGGATCCCA+TGG 0.630077 4:+61355202 MsaT020965.1:CDS
ATCATCTGTTGCTGGTGTGG+AGG 0.632127 4:+61352919 MsaT020965.1:CDS
ATATTGTTACAAATATAAGG+AGG 0.636102 4:-61353040 None:intergenic
CTAGTGCCAAGTTTGCGCGA+AGG 0.646989 4:+61352174 MsaT020965.1:intron
AGAAAATAAGGAGCGACCGG+AGG 0.647366 4:+61353422 MsaT020965.1:CDS
GTTGCTGTCAAGGAATGCAG+TGG 0.651362 4:-61352509 None:intergenic
TCTGGACTATGATAAGCCAT+GGG 0.651572 4:-61355218 None:intergenic
TATTGTTACAAATATAAGGA+GGG 0.653546 4:-61353039 None:intergenic
TACTTCCGGAAAAGTACAAG+AGG 0.653833 4:+61352642 MsaT020965.1:CDS
AGAGGTTGCAACAACTGAGG+AGG 0.662600 4:+61355166 MsaT020965.1:CDS
ATGACCGTGATAAAAGCTCA+AGG 0.662857 4:+61352942 MsaT020965.1:CDS
TTATTCGTAAAGGATGATGT+CGG 0.671321 4:-61354377 None:intergenic
CAGCAGAGCCTGGTCCACTG+TGG 0.673933 4:-61353343 None:intergenic
GGAAGAGGTTGCAACAACTG+AGG 0.674245 4:+61355163 MsaT020965.1:intron
TGCTGGTGCTAATAGTGAAG+AGG 0.679934 4:+61353955 MsaT020965.1:CDS
ACTTGAAGAAAATGATGACG+AGG 0.685696 4:+61353069 MsaT020965.1:CDS
GTTCTTCCCATCTACAGACG+AGG 0.708904 4:-61352248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 61352014 61355301 61352014 ID=MsaG020965
Chr4 mRNA 61352014 61355301 61352014 ID=MsaT020965.1;Parent=MsaG020965
Chr4 exon 61352014 61352059 61352014 ID=MsaT020965.1.exon1;Parent=MsaT020965.1
Chr4 CDS 61352014 61352059 61352014 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61352185 61352331 61352185 ID=MsaT020965.1.exon2;Parent=MsaT020965.1
Chr4 CDS 61352185 61352331 61352185 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61352470 61352663 61352470 ID=MsaT020965.1.exon3;Parent=MsaT020965.1
Chr4 CDS 61352470 61352663 61352470 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61352878 61353263 61352878 ID=MsaT020965.1.exon4;Parent=MsaT020965.1
Chr4 CDS 61352878 61353263 61352878 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61353332 61353443 61353332 ID=MsaT020965.1.exon5;Parent=MsaT020965.1
Chr4 CDS 61353332 61353443 61353332 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61353549 61353580 61353549 ID=MsaT020965.1.exon6;Parent=MsaT020965.1
Chr4 CDS 61353549 61353580 61353549 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61353934 61354138 61353934 ID=MsaT020965.1.exon7;Parent=MsaT020965.1
Chr4 CDS 61353934 61354138 61353934 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61354282 61354426 61354282 ID=MsaT020965.1.exon8;Parent=MsaT020965.1
Chr4 CDS 61354282 61354426 61354282 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61354562 61354659 61354562 ID=MsaT020965.1.exon9;Parent=MsaT020965.1
Chr4 CDS 61354562 61354659 61354562 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Chr4 exon 61355164 61355301 61355164 ID=MsaT020965.1.exon10;Parent=MsaT020965.1
Chr4 CDS 61355164 61355301 61355164 ID=cds.MsaT020965.1;Parent=MsaT020965.1
Gene Sequence

>MsaT020965.1

ATGGATCTTTTTGATGATATTCTACTTGAAGCTCCTGCTGCACGTGTTTGCGCGAAGGTCAAATCAAAACAGTCCTTACGAAAGGAAATATCTGCATCAGAACATGCTACCTCGTCTGTAGATGGGAAGAACATGCATGTTGCTTCAACCCCCACCGTCACTAAATCCATTCCCGAGACCAACCCCAATAATGATAGTGCAGAGAGTGAATTGAATAAAGTGACTTCCCCATCCACTGCATTCCTTGACAGCAACAAAAGTTTGCAAGTTAATAACTCATTGCAAGACAGCCTAAATGTTGGCTTCAGAAGTGCTTCAGGCGACAATAGCACTGCAATTTCCGAGAACAACATTCATTCCATTTTTACTTCCGGAAAAGTACAAGAGGATGCTAATGAGAGGAAAAAATCTCTAAGGAAGCGTAAAAGATCATCTGTTGCTGGTGTGGAGGATGACCGTGATAAAAGCTCAAGGCAGCTGAGAAAACAAGCAGCTTGTGAACATGTTAAAAACTCAAACTCATTAATTGAGGATGATGATGGACTCGACCCTCCTTATATTTGTAACAATATAGATCAACTTGAAGAAAATGATGACGAGGACGAAGTGGATAATTCATCTAAGAAGAAAAGAGCATTAGCAAGTTCAAAAAAGAAATATGTGGCTAAAAATGGAAAAGCATACAAAAAAAGCAAGAAGGAAAATGGCGATTCAGAAAAACTACTGAACCACCCAAAAGTTCTCTCATTCTTCTCGGCGAAGAAAAAGACAAGTATTTTCATTCCCACAGTGGACCAGGCTCTGCTGGATGATTATGAACTTGGTTCTCAATCAATTTCTCTTAGGGATATTCTTGCAATTGCAGAAAATAAGGAGCGACCGGAGAAAAAGGAGGCAAAATCTTCCACCAATCGAAGTGGTGGGGATTTCTTTCATGATGCTGGTGCTAATAGTGAAGAGGAGACTTTTAGTTCAGATGATGATGGTTATAGAGACCAAGAGTATGATCAAGCTAGTAAAAAGTTTGCATCAACTGTCGCCGCTTTATACAATTCACATTCTTTTAGGGACAAGTCACCTAGGGTAAAATGGTCGAAACAAGATACCGAAAAGTTTTATGAGGCTCTGAAGGAGTTTGGCCTAGATTTTACAATGATTCAACAAATTTTTTCTGGTAAATCAAGACGTCAAATTATGCTGAAGTTCAAGAATGAAGACCGACATCATCCTTTACGAATAACTGATGCTATTAATAGTCGTTCATCAGATCATCATATTTTTAAGTCGGTGATTGCGAAACTGCCACAAATTTCTACCACTAATGCAAATCAGGAAGCCACTGAAGACATGTCACCTGGAATTAATGAAGAGGTTGCAACAACTGAGGAGGACAGTGCTGATGTTAAAGATCAGGAGGATCCCATGGCTTATCATAGTCCAGAGCAATCTGATGATAGTGATGATGACTTACGGAAATGGTCTCAGTATCAAAGTGTTATTTAG

Protein sequence

>MsaT020965.1

MDLFDDILLEAPAARVCAKVKSKQSLRKEISASEHATSSVDGKNMHVASTPTVTKSIPETNPNNDSAESELNKVTSPSTAFLDSNKSLQVNNSLQDSLNVGFRSASGDNSTAISENNIHSIFTSGKVQEDANERKKSLRKRKRSSVAGVEDDRDKSSRQLRKQAACEHVKNSNSLIEDDDGLDPPYICNNIDQLEENDDEDEVDNSSKKKRALASSKKKYVAKNGKAYKKSKKENGDSEKLLNHPKVLSFFSAKKKTSIFIPTVDQALLDDYELGSQSISLRDILAIAENKERPEKKEAKSSTNRSGGDFFHDAGANSEEETFSSDDDGYRDQEYDQASKKFASTVAALYNSHSFRDKSPRVKWSKQDTEKFYEALKEFGLDFTMIQQIFSGKSRRQIMLKFKNEDRHHPLRITDAINSRSSDHHIFKSVIAKLPQISTTNANQEATEDMSPGINEEVATTEEDSADVKDQEDPMAYHSPEQSDDSDDDLRKWSQYQSVI*