AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020968


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020968 MtrunA17_Chr4g0033611 95.294 255 11 1 7 260 3 257 1.16e-173 478
MsaG020968 MtrunA17_Chr2g0329421 69.903 206 57 4 53 256 1 203 6.54e-100 290
MsaG020968 MtrunA17_Chr3g0078041 43.684 190 97 3 73 258 44 227 6.55e-44 148
MsaG020968 MtrunA17_Chr2g0329361 36.923 195 105 6 71 251 53 243 4.97e-35 126
MsaG020968 MtrunA17_Chr7g0233911 36.559 186 105 4 71 251 26 203 2.43e-33 120
MsaG020968 MtrunA17_Chr5g0448761 50.862 116 52 3 71 181 52 167 1.62e-22 96.7
MsaG020968 MtrunA17_Chr2g0287061 42.202 109 51 1 78 186 182 278 1.47e-21 94.0
MsaG020968 MtrunA17_Chr2g0287061 37.255 102 52 1 78 179 28 117 1.97e-16 78.6
MsaG020968 MtrunA17_Chr3g0082121 44.860 107 53 3 79 181 43 147 4.85e-20 89.4
MsaG020968 MtrunA17_Chr1g0203721 36.943 157 87 5 50 197 27 180 5.02e-20 89.4
MsaG020968 MtrunA17_Chr5g0401121 42.593 108 56 3 79 182 56 161 1.54e-19 83.2
MsaG020968 MtrunA17_Chr6g0458391 38.393 112 67 2 79 189 225 335 2.87e-19 87.0
MsaG020968 MtrunA17_Chr6g0458391 36.283 113 67 1 70 177 77 189 1.97e-15 75.9
MsaG020968 MtrunA17_Chr1g0202341 33.582 134 77 3 53 179 25 153 1.99e-18 84.7
MsaG020968 MtrunA17_Chr5g0440451 39.216 102 50 2 78 179 42 131 9.92e-16 76.6
MsaG020968 MtrunA17_Chr2g0291581 34.328 134 83 2 79 211 71 200 3.54e-15 75.1
MsaG020968 MtrunA17_Chr2g0291591 34.615 104 65 1 78 181 63 163 6.26e-15 73.9
MsaG020968 MtrunA17_Chr3g0080351 30.345 145 86 3 58 187 54 198 3.00e-14 72.4
MsaG020968 MtrunA17_Chr7g0258861 37.383 107 63 3 78 181 9 114 1.79e-12 66.2
MsaG020968 MtrunA17_Chr1g0207551 29.134 127 88 2 57 182 11 136 1.08e-11 64.7
MsaG020968 MtrunA17_Chr2g0329031 32.143 112 70 3 75 184 10 117 3.08e-11 63.2
MsaG020968 MtrunA17_Chr1g0171741 31.285 179 105 9 12 179 64 235 8.06e-11 60.8
MsaG020968 MtrunA17_Chr2g0276841 28.636 220 131 11 50 255 42 249 9.63e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020968 AT3G59470.3 59.227 233 84 4 36 260 20 249 7.47e-95 279
MsaG020968 AT3G59470.1 59.227 233 84 4 36 260 20 249 7.47e-95 279
MsaG020968 AT3G59470.2 59.227 233 84 4 36 260 32 261 1.13e-94 279
MsaG020968 AT3G07500.1 42.938 177 98 3 78 251 32 208 2.69e-44 149
MsaG020968 AT2G43280.1 38.571 210 105 5 47 251 2 192 2.20e-37 131
MsaG020968 AT4G12850.1 35.714 182 109 3 73 253 5 179 6.30e-32 116
MsaG020968 AT2G43280.2 39.877 163 86 4 94 251 2 157 5.04e-31 113
MsaG020968 AT4G38180.1 54.630 108 45 2 78 181 72 179 1.63e-24 102
MsaG020968 AT4G12850.2 40.650 123 70 2 73 194 5 125 3.37e-24 94.7
MsaG020968 AT4G12850.4 42.056 107 60 1 73 179 5 109 8.70e-24 94.0
MsaG020968 AT5G18960.2 36.184 152 71 2 36 187 14 139 7.17e-23 98.2
MsaG020968 AT5G18960.2 37.963 108 55 1 75 182 207 302 8.66e-20 89.0
MsaG020968 AT5G18960.1 36.184 152 71 2 36 187 14 139 7.17e-23 98.2
MsaG020968 AT5G18960.1 37.963 108 55 1 75 182 207 302 8.66e-20 89.0
MsaG020968 AT3G06250.2 38.793 116 59 1 76 191 187 290 5.47e-21 92.4
MsaG020968 AT3G06250.2 40.952 105 50 1 75 179 24 116 2.02e-20 90.9
MsaG020968 AT3G06250.1 38.793 116 59 1 76 191 187 290 5.47e-21 92.4
MsaG020968 AT3G06250.1 40.952 105 50 1 75 179 24 116 2.02e-20 90.9
MsaG020968 AT3G06250.3 38.793 116 59 1 76 191 187 290 5.47e-21 92.4
MsaG020968 AT3G06250.3 40.952 105 50 1 75 179 24 116 2.02e-20 90.9
MsaG020968 AT1G52520.1 38.835 103 60 1 77 179 83 182 1.31e-17 82.4
MsaG020968 AT1G80010.1 37.736 106 63 1 76 181 92 194 2.21e-16 79.0
MsaG020968 AT2G32250.5 38.182 110 62 2 74 181 33 138 4.51e-15 75.1
MsaG020968 AT2G32250.6 38.182 110 62 2 74 181 33 138 4.76e-15 75.1
MsaG020968 AT2G32250.1 38.182 110 62 2 74 181 33 138 4.76e-15 75.1
MsaG020968 AT2G32250.3 38.182 110 62 2 74 181 33 138 4.76e-15 75.1
MsaG020968 AT2G32250.2 38.182 110 62 2 74 181 33 138 4.85e-15 75.1
MsaG020968 AT2G32250.4 38.182 110 62 2 74 181 33 138 4.85e-15 75.1
MsaG020968 AT4G19990.7 28.966 145 75 2 82 212 38 168 2.20e-14 73.2
MsaG020968 AT4G19990.6 28.966 145 75 2 82 212 24 154 2.22e-14 73.2
MsaG020968 AT4G19990.2 28.966 145 75 2 82 212 24 154 2.22e-14 73.2
MsaG020968 AT4G19990.5 28.966 145 75 2 82 212 24 154 2.29e-14 73.2
MsaG020968 AT4G19990.3 28.966 145 75 2 82 212 24 154 2.29e-14 73.2
MsaG020968 AT4G19990.4 28.966 145 75 2 82 212 24 154 2.29e-14 73.2
MsaG020968 AT2G32250.7 39.000 100 55 2 84 181 2 97 4.38e-14 72.0
MsaG020968 AT2G32250.8 39.000 100 55 2 84 181 2 97 4.59e-14 72.0
MsaG020968 AT3G22170.2 28.859 149 89 4 54 187 46 192 1.25e-11 65.1
MsaG020968 AT3G22170.1 28.859 149 89 4 54 187 46 192 1.25e-11 65.1

Find 63 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAAATTAATTCTGGTAAA+TGG 0.122359 4:-61369032 MsaT020968.1:CDS
TTATGTTCTTCTTCTTCTTT+AGG 0.197944 4:+61368122 None:intergenic
TCTGTATCGTTCTATGTATC+TGG 0.244963 4:-61369604 None:intergenic
CAGAAACAAGGGTTGGTTGC+AGG 0.300475 4:-61369071 MsaT020968.1:CDS
TCTATGTATCTGGGCTTCTT+AGG 0.304144 4:-61369594 MsaT020968.1:intron
GAAAATTAATTCTGGTAAAT+GGG 0.333770 4:-61369031 MsaT020968.1:CDS
ATGATGAGGAAAATTAATTC+TGG 0.337234 4:-61369039 MsaT020968.1:CDS
ATCTCCTTTCTACTACTTCC+AGG 0.346138 4:+61368967 None:intergenic
GAGATTGATGACTCTGTTAA+CGG 0.380618 4:-61369480 MsaT020968.1:CDS
AGGAAAGACGGAACTGCTAT+TGG 0.388968 4:-61369165 MsaT020968.1:CDS
AACGTGACTCAAGATTCTTC+TGG 0.391633 4:-61369354 MsaT020968.1:CDS
CTTGAGTCACGTTATCATCT+TGG 0.395456 4:+61369364 None:intergenic
TCTTCTTCCATGTATTCATC+AGG 0.412222 4:+61369414 None:intergenic
TCTTGGTCTTGTTTGTAAGA+TGG 0.422563 4:+61369381 None:intergenic
TCTACTACTTCCAGGATTCA+AGG 0.432111 4:+61368975 None:intergenic
GGAGATGTTTGAAATTTATC+CGG 0.433714 4:-61368950 MsaT020968.1:intron
TTCTGCTGAGGAGCCTTATG+TGG 0.437415 4:-61369286 MsaT020968.1:CDS
GGCTCCTCAGCAGAAGCGTC+TGG 0.448272 4:+61369294 None:intergenic
TGAGGAGCCTTATGTGGGTC+AGG 0.449928 4:-61369280 MsaT020968.1:CDS
TAAGCAAGCTCTCACGATCA+AGG 0.471737 4:-61369185 MsaT020968.1:CDS
CACACACATCCCTTGAATCC+TGG 0.472295 4:-61368985 MsaT020968.1:CDS
CCAACTGCTGAGATAGTTCG+CGG 0.495662 4:+61368273 None:intergenic
CTGTATCGTTCTATGTATCT+GGG 0.499393 4:-61369603 None:intergenic
TCGAACTCCTGACCCACATA+AGG 0.503181 4:+61369273 None:intergenic
CTACTACTTCCAGGATTCAA+GGG 0.508347 4:+61368976 None:intergenic
AGAAACAAGGGTTGGTTGCA+GGG 0.508414 4:-61369070 MsaT020968.1:CDS
GGTTATTACGAAGTTTGTAA+AGG 0.521568 4:-61369010 MsaT020968.1:CDS
AGCAGAAGCGTCTGGTAGTG+TGG 0.524624 4:+61369302 None:intergenic
AATGCATACGCAGCCGAGGT+TGG 0.541461 4:-61369222 MsaT020968.1:CDS
CGAAAAGATTGTGAGGCAAA+GGG 0.544339 4:-61369094 MsaT020968.1:CDS
GCGAAAAGATTGTGAGGCAA+AGG 0.552809 4:-61369095 MsaT020968.1:CDS
AGCATAATGTGGGTCTCTCA+AGG 0.559262 4:-61368184 MsaT020968.1:CDS
GGCTCTTGTTTGCAACAAAG+AGG 0.560233 4:-61369139 MsaT020968.1:CDS
AGTAGACAGTGTGAAGGAGA+TGG 0.569154 4:-61368147 MsaT020968.1:CDS
GAATCCTGGAAGTAGTAGAA+AGG 0.569556 4:-61368971 MsaT020968.1:CDS
GATGGTGCCATGGTTGTAGC+TGG 0.569629 4:-61369444 MsaT020968.1:CDS
CCGCGAACTATCTCAGCAGT+TGG 0.570583 4:-61368273 MsaT020968.1:CDS
TGGTAGTGTGGGAACTGCAG+TGG 0.577611 4:+61369314 None:intergenic
GCTCTTGTTTGCAACAAAGA+GGG 0.580043 4:-61369138 MsaT020968.1:CDS
TGAGGCAAAGGGCAGAAACA+AGG 0.583136 4:-61369083 MsaT020968.1:CDS
TAGCATACCTGATGAATACA+TGG 0.584054 4:-61369421 MsaT020968.1:CDS
GCAGAAGCGTCTGGTAGTGT+GGG 0.584434 4:+61369303 None:intergenic
GGTAGTGTGGGAACTGCAGT+GGG 0.584997 4:+61369315 None:intergenic
AGACGGAACTGCTATTGGAC+GGG 0.589838 4:-61369160 MsaT020968.1:CDS
CTCTCACGATCAAGGAAAGA+CGG 0.590017 4:-61369177 MsaT020968.1:CDS
AAGACGGAACTGCTATTGGA+CGG 0.612304 4:-61369161 MsaT020968.1:CDS
TCTGCTGAGGAGCCTTATGT+GGG 0.621782 4:-61369285 MsaT020968.1:CDS
AGTGATATTTGATTATATCG+AGG 0.622955 4:-61368207 MsaT020968.1:CDS
CAAAGGGCAGAAACAAGGGT+TGG 0.628129 4:-61369078 MsaT020968.1:CDS
TTACAATGCATACGCAGCCG+AGG 0.629238 4:-61369226 MsaT020968.1:CDS
TATGCTACCAGCTACAACCA+TGG 0.631572 4:+61369437 None:intergenic
GCGTATAGTAGACAGTGTGA+AGG 0.633138 4:-61368153 MsaT020968.1:CDS
TACAGTAAGAGATGGTGCCA+TGG 0.635293 4:-61369454 MsaT020968.1:CDS
TTATATCGAGGAGCATAATG+TGG 0.635828 4:-61368195 MsaT020968.1:CDS
TATATCGAGGAGCATAATGT+GGG 0.635920 4:-61368194 MsaT020968.1:CDS
ACTACCAGACGCTTCTGCTG+AGG 0.646967 4:-61369298 MsaT020968.1:CDS
AACGGCGATACAGTAAGAGA+TGG 0.652494 4:-61369462 MsaT020968.1:CDS
GAGGCAAAGGGCAGAAACAA+GGG 0.664007 4:-61369082 MsaT020968.1:CDS
GCAGGGCAATGATTATGATG+AGG 0.664420 4:-61369053 MsaT020968.1:CDS
ACAAGCGCGAAAAGATTGTG+AGG 0.665448 4:-61369101 MsaT020968.1:CDS
GTGACTCAAGATTCTTCTGG+AGG 0.668755 4:-61369351 MsaT020968.1:CDS
TGTGGGTCAGGAGTTCGAGT+CGG 0.669514 4:-61369268 MsaT020968.1:CDS
GCGTATGATGAATCCAACCT+CGG 0.694329 4:+61369209 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 61368115 61369613 61368115 ID=MsaG020968
Chr4 mRNA 61368115 61369613 61368115 ID=MsaT020968.1;Parent=MsaG020968
Chr4 exon 61368115 61368318 61368115 ID=MsaT020968.1.exon3;Parent=MsaT020968.1
Chr4 CDS 61368115 61368318 61368115 ID=cds.MsaT020968.1;Parent=MsaT020968.1
Chr4 exon 61368951 61369510 61368951 ID=MsaT020968.1.exon2;Parent=MsaT020968.1
Chr4 CDS 61368951 61369510 61368951 ID=cds.MsaT020968.1;Parent=MsaT020968.1
Chr4 exon 61369595 61369613 61369595 ID=MsaT020968.1.exon1;Parent=MsaT020968.1
Chr4 CDS 61369595 61369613 61369595 ID=cds.MsaT020968.1;Parent=MsaT020968.1
Gene Sequence

>MsaT020968.1

ATGTATCTGGGCTTCTTAGTGGAGTTTGAGATTGATGACTCTGTTAACGGCGATACAGTAAGAGATGGTGCCATGGTTGTAGCTGGTAGCATACCTGATGAATACATGGAAGAAGAAAGTTCTGAACCATCTTACAAACAAGACCAAGATGATAACGTGACTCAAGATTCTTCTGGAGGAGTGTTAACAATACCCACTGCAGTTCCCACACTACCAGACGCTTCTGCTGAGGAGCCTTATGTGGGTCAGGAGTTCGAGTCGGAAGCAGCAGCGCATGCATTTTACAATGCATACGCAGCCGAGGTTGGATTCATCATACGCGTAAGCAAGCTCTCACGATCAAGGAAAGACGGAACTGCTATTGGACGGGCTCTTGTTTGCAACAAAGAGGGTTACAGAATGCCTGACAAGCGCGAAAAGATTGTGAGGCAAAGGGCAGAAACAAGGGTTGGTTGCAGGGCAATGATTATGATGAGGAAAATTAATTCTGGTAAATGGGTTATTACGAAGTTTGTAAAGGAGCACACACATCCCTTGAATCCTGGAAGTAGTAGAAAGGAGATGTTTGAAATTTATCCGTCTCAGAACGAACATGATAAAATCCGCGAACTATCTCAGCAGTTGGCAATAGAGAAAAAGCGATCTGTGACATATAAAAGACAACTTGAAGTGATATTTGATTATATCGAGGAGCATAATGTGGGTCTCTCAAGGAAAATGCAGCGTATAGTAGACAGTGTGAAGGAGATGGAACCTAAAGAAGAAGAAGAACATAATCATTGA

Protein sequence

>MsaT020968.1

MYLGFLVEFEIDDSVNGDTVRDGAMVVAGSIPDEYMEEESSEPSYKQDQDDNVTQDSSGGVLTIPTAVPTLPDASAEEPYVGQEFESEAAAHAFYNAYAAEVGFIIRVSKLSRSRKDGTAIGRALVCNKEGYRMPDKREKIVRQRAETRVGCRAMIMMRKINSGKWVITKFVKEHTHPLNPGSSRKEMFEIYPSQNEHDKIRELSQQLAIEKKRSVTYKRQLEVIFDYIEEHNVGLSRKMQRIVDSVKEMEPKEEEEHNH*