AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG020971

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020971 MtrunA17_Chr4g0033641 99.167 240 2 0 1 240 1 240 7.21e-179 490
MsaG020971 MtrunA17_Chr2g0329511 69.262 244 70 3 1 240 1 243 6.70e-117 333
MsaG020971 MtrunA17_Chr2g0314671 60.550 109 43 0 1 109 1 109 7.98e-43 146
MsaG020971 MtrunA17_Chr1g0155811 34.799 273 133 8 1 240 1 261 8.15e-38 133
MsaG020971 MtrunA17_Chr3g0145061 54.717 106 47 1 1 105 1 106 1.41e-35 126
MsaG020971 MtrunA17_Chr4g0035651 49.558 113 56 1 1 112 1 113 1.72e-35 125
MsaG020971 MtrunA17_Chr3g0142221 52.830 106 48 1 1 104 1 106 1.12e-32 118
MsaG020971 MtrunA17_Chr5g0405151 42.537 134 71 4 1 131 1 131 4.28e-25 97.8
MsaG020971 MtrunA17_Chr7g0220721 43.478 92 43 1 5 96 26 108 7.10e-19 84.7
MsaG020971 MtrunA17_Chr7g0220721 40.845 71 36 1 5 75 69 133 8.40e-11 61.2
MsaG020971 MtrunA17_Chr3g0136701 43.750 96 45 1 5 100 6 92 1.45e-18 82.4
MsaG020971 MtrunA17_Chr1g0150471 42.391 92 44 1 5 96 6 88 1.93e-18 82.4
MsaG020971 MtrunA17_Chr4g0072871 39.130 92 47 1 5 96 22 104 9.33e-17 78.6
MsaG020971 MtrunA17_Chr7g0268691 41.837 98 47 2 5 102 20 107 7.17e-16 75.9
MsaG020971 MtrunA17_Chr5g0429341 35.417 96 53 1 1 96 1 87 3.61e-14 71.2
MsaG020971 MtrunA17_Chr3g0120711 38.542 96 49 2 1 96 1 86 5.05e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG020971 AT1G06040.1 62.500 248 85 4 1 240 1 248 9.03e-105 303
MsaG020971 AT2G31380.1 59.836 244 88 7 1 240 1 238 3.20e-92 271
MsaG020971 AT1G06040.2 66.071 168 51 3 1 162 1 168 2.76e-70 213
MsaG020971 AT1G78600.1 56.800 125 52 1 1 125 1 123 5.74e-42 145
MsaG020971 AT1G78600.2 56.800 125 52 1 1 125 1 123 1.23e-41 144
MsaG020971 AT4G10240.1 47.619 147 64 4 1 141 1 140 2.44e-36 126
MsaG020971 AT1G75540.1 56.522 115 47 2 1 112 1 115 5.63e-36 130
MsaG020971 AT4G39070.1 45.890 146 76 2 1 145 1 144 8.44e-34 122
MsaG020971 AT2G21320.1 50.000 104 49 2 1 104 1 101 4.36e-25 97.8
MsaG020971 AT4G38960.1 42.424 132 68 3 1 127 1 129 6.52e-25 97.4
MsaG020971 AT4G38960.6 42.424 132 68 3 1 127 1 129 6.52e-25 97.4
MsaG020971 AT5G15850.1 33.143 175 90 4 5 163 12 175 8.24e-20 87.4
MsaG020971 AT2G24790.2 43.478 92 43 1 5 96 8 90 1.23e-18 82.0
MsaG020971 AT2G24790.1 43.478 92 43 1 5 96 8 90 1.33e-18 83.2
MsaG020971 AT3G02380.1 43.478 92 43 1 5 96 16 98 1.59e-17 80.9
MsaG020971 AT5G15840.2 35.200 125 72 1 5 129 20 135 2.40e-17 79.3
MsaG020971 AT5G15840.1 35.200 125 72 1 5 129 20 135 2.46e-17 80.5
MsaG020971 AT4G38960.3 32.000 175 68 4 1 127 1 172 5.73e-17 77.4
MsaG020971 AT5G24930.1 41.667 96 47 1 5 100 6 92 7.92e-17 79.0
MsaG020971 AT2G47890.3 42.353 85 40 1 5 89 13 88 2.23e-14 71.6
MsaG020971 AT2G47890.1 42.353 85 40 1 5 89 13 88 2.27e-14 71.6
MsaG020971 AT2G47890.2 42.353 85 40 1 5 89 13 88 4.31e-14 69.7
MsaG020971 AT1G28050.1 34.653 101 57 1 2 102 6 97 3.95e-13 68.6
MsaG020971 AT1G68190.2 34.375 96 52 2 1 96 39 123 7.36e-13 67.8
MsaG020971 AT1G68190.1 34.375 96 52 2 1 96 10 94 8.24e-13 67.4
MsaG020971 AT1G68190.3 34.375 96 52 2 1 96 10 94 8.24e-13 67.4
MsaG020971 AT2G33500.1 34.000 100 57 1 3 102 10 100 1.22e-12 67.0
MsaG020971 AT2G33500.2 34.000 100 57 1 3 102 10 100 1.27e-12 67.0
MsaG020971 AT3G07650.3 37.647 85 44 1 1 85 1 76 1.42e-12 66.6
MsaG020971 AT3G07650.2 37.647 85 44 1 1 85 1 76 1.42e-12 66.6
MsaG020971 AT3G07650.4 37.647 85 44 1 1 85 1 76 1.42e-12 66.6
MsaG020971 AT3G07650.1 37.647 85 44 1 1 85 1 76 1.42e-12 66.6
MsaG020971 AT5G48250.1 32.653 98 55 2 1 96 1 89 1.98e-11 63.5
MsaG020971 AT5G57660.1 33.333 93 49 2 4 96 21 100 2.69e-11 62.8

Find 51 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGTAGCTGGTAGTCTTTC+TGG 0.109250 4:-61389203 MsaT020971.1:CDS
TTGGGCTGTTGATGATTTCT+TGG 0.216145 4:-61389021 MsaT020971.1:CDS
GATGATTTCTTGGAATTAAC+TGG 0.278064 4:-61389011 MsaT020971.1:CDS
CAGAAGCAATCCATGGAGTT+TGG 0.289917 4:-61388405 MsaT020971.1:CDS
ACTTGGCAGATATCACATTT+AGG 0.310599 4:+61390190 None:intergenic
GTAAGACATATAAGATTTAG+AGG 0.311347 4:+61388260 None:intergenic
ACACTGCCAGCATGCATTAC+TGG 0.340744 4:+61388297 None:intergenic
TACCGGTATCCGTGTGGCTT+TGG 0.344898 4:-61389159 MsaT020971.1:CDS
ACGGGAACTTGTTGCGTGTC+TGG 0.356267 4:+61389086 None:intergenic
TAAAGTTGTCTTACCTTGTC+TGG 0.367338 4:+61388978 None:intergenic
TGCCTAGTTTCGCATCCTCT+TGG 0.378192 4:-61389040 MsaT020971.1:CDS
GCCTAGTTTCGCATCCTCTT+GGG 0.385410 4:-61389039 MsaT020971.1:CDS
AGACTACCAGCTACATGAAT+TGG 0.402532 4:+61389209 None:intergenic
CTAAACCTTCCTGAGGAAAC+TGG 0.421815 4:+61388343 None:intergenic
TAAGATTTAGAGGCTTTGTA+AGG 0.437114 4:+61388270 None:intergenic
CGATGATGATGAGCACTTCA+TGG 0.441777 4:-61388205 MsaT020971.1:CDS
CATGTGCTTTAGCCGAGATC+TGG 0.447542 4:+61388180 None:intergenic
TGGAGTTTGGAGAGCTTGAA+TGG 0.451045 4:-61388392 MsaT020971.1:CDS
TGTTGCGTGTCTGGATTAGA+TGG 0.466529 4:+61389095 None:intergenic
AGCCAAAGCCACACGGATAC+CGG 0.489165 4:+61389157 None:intergenic
GCACAACAAATCACCGTAGC+AGG 0.495596 4:+61390319 None:intergenic
CTGGATTAGATGGTTCAACT+TGG 0.497438 4:+61389105 None:intergenic
TTTCAATGACCAGTTTCCTC+AGG 0.500064 4:-61388352 MsaT020971.1:CDS
CTGAGGAGGAGAAACTTTGA+CGG 0.504586 4:+61389067 None:intergenic
CACTTCATGGTGCCAGATCT+CGG 0.507917 4:-61388192 MsaT020971.1:CDS
TACCGGTAGCAAGGAAGCGC+TGG 0.512715 4:+61389174 None:intergenic
CTTGAATGGCTATCGGATGC+AGG 0.516958 4:-61388378 MsaT020971.1:CDS
TCAACACAAAATATGAAAGC+TGG 0.522233 4:+61389266 None:intergenic
GCCCAAGAGGATGCGAAACT+AGG 0.529539 4:+61389038 None:intergenic
TCATGTCGGACTTCAATCCT+AGG 0.531676 4:+61388231 None:intergenic
AGACCGAGCACTGTTCTGTA+AGG 0.533969 4:-61389243 MsaT020971.1:CDS
AGCTTGCTAGCAAGCATCAA+AGG 0.550543 4:-61390243 MsaT020971.1:CDS
TTCAAGCTCTCCAAACTCCA+TGG 0.552963 4:+61388395 None:intergenic
CACCAGCGCTTCCTTGCTAC+CGG 0.553385 4:-61389176 MsaT020971.1:CDS
TGGAGAGCTTGAATGGCTAT+CGG 0.557707 4:-61388385 MsaT020971.1:CDS
AAACTAGGCACTTGCTGAGG+AGG 0.565089 4:+61389053 None:intergenic
CAACTTCCAGTAATGCATGC+TGG 0.570734 4:-61388303 MsaT020971.1:CDS
TGAGGAGGAGAAACTTTGAC+GGG 0.575513 4:+61389068 None:intergenic
AATGACCAGTTTCCTCAGGA+AGG 0.575639 4:-61388348 MsaT020971.1:CDS
CATCATCATCGTCTTCATGT+CGG 0.589449 4:+61388217 None:intergenic
CGGTAGCAAGGAAGCGCTGG+TGG 0.597801 4:+61389177 None:intergenic
GAAATCATCAACAGCCCAAG+AGG 0.601713 4:+61389025 None:intergenic
GATGAACCAATTCATGTAGC+TGG 0.610215 4:-61389215 MsaT020971.1:CDS
TGGCTTTGATTCACCAGACA+AGG 0.610663 4:-61388991 MsaT020971.1:intron
GCATGCATTACTGGAAGTTG+CGG 0.617995 4:+61388306 None:intergenic
CCACACGGATACCGGTAGCA+AGG 0.621121 4:+61389165 None:intergenic
AACTAGAAGCCAAAGCCACA+CGG 0.621527 4:+61389150 None:intergenic
AGTCCTTACAGAACAGTGCT+CGG 0.631276 4:+61389240 None:intergenic
CCTTGCTACCGGTATCCGTG+TGG 0.645262 4:-61389165 MsaT020971.1:CDS
GCGAAACTAGGCACTTGCTG+AGG 0.649769 4:+61389050 None:intergenic
GCTGCAGCTAAACCTTCCTG+AGG 0.726877 4:+61388336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 61388188 61390374 61388188 ID=MsaG020971
Chr4 mRNA 61388188 61390374 61388188 ID=MsaT020971.1;Parent=MsaG020971
Chr4 exon 61388188 61388424 61388188 ID=MsaT020971.1.exon3;Parent=MsaT020971.1
Chr4 CDS 61388188 61388424 61388188 ID=cds.MsaT020971.1;Parent=MsaT020971.1
Chr4 exon 61388992 61389294 61388992 ID=MsaT020971.1.exon2;Parent=MsaT020971.1
Chr4 CDS 61388992 61389294 61388992 ID=cds.MsaT020971.1;Parent=MsaT020971.1
Chr4 exon 61390192 61390374 61390192 ID=MsaT020971.1.exon1;Parent=MsaT020971.1
Chr4 CDS 61390192 61390374 61390192 ID=cds.MsaT020971.1;Parent=MsaT020971.1
Gene Sequence

>MsaT020971.1

ATGAAAATACAGTGTGATGTGTGTGAAAAAGCTCCTGCTACGGTGATTTGTTGTGCTGATGAAGCTGCTTTGTGTGCTAAATGTGATGTTGAAGTTCATGCTGCTAATAAGCTTGCTAGCAAGCATCAAAGGCTTCTTCTTCAATGTCTTTCTAACAAGCTTCCTAAATGTGATATCTGCCAAGATAAGCCAGCTTTCATATTTTGTGTTGAAGACCGAGCACTGTTCTGTAAGGACTGTGATGAACCAATTCATGTAGCTGGTAGTCTTTCTGGAAACCACCAGCGCTTCCTTGCTACCGGTATCCGTGTGGCTTTGGCTTCTAGTTGCACCAAAGACAATGAGAAAAGCCAAGTTGAACCATCTAATCCAGACACGCAACAAGTTCCCGTCAAAGTTTCTCCTCCTCAGCAAGTGCCTAGTTTCGCATCCTCTTGGGCTGTTGATGATTTCTTGGAATTAACTGGCTTTGATTCACCAGACAAGAAGCAATCCATGGAGTTTGGAGAGCTTGAATGGCTATCGGATGCAGGTCTTTTCAATGACCAGTTTCCTCAGGAAGGTTTAGCTGCAGCTGAAGTTCCGCAACTTCCAGTAATGCATGCTGGCAGTGTTTATCCTTACAAAGCCTCTAAATCTTATATGTCTTACAAAAAGCCTAGGATTGAAGTCCGACATGAAGACGATGATGATGAGCACTTCATGGTGCCAGATCTCGGCTAA
Protein sequence

>MsaT020971.1

MKIQCDVCEKAPATVICCADEAALCAKCDVEVHAANKLASKHQRLLLQCLSNKLPKCDICQDKPAFIFCVEDRALFCKDCDEPIHVAGSLSGNHQRFLATGIRVALASSCTKDNEKSQVEPSNPDTQQVPVKVSPPQQVPSFASSWAVDDFLELTGFDSPDKKQSMEFGELEWLSDAGLFNDQFPQEGLAAAEVPQLPVMHAGSVYPYKASKSYMSYKKPRIEVRHEDDDDEHFMVPDLG*