AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021073

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021073 MtrunA17_Chr1g0167311 86.250 80 11 0 86 165 156 235 5.84e-41 146
MsaG021073 MtrunA17_Chr7g0261491 73.684 95 24 1 86 179 161 255 1.13e-39 143
MsaG021073 MtrunA17_Chr1g0183651 73.684 95 24 1 86 179 161 255 1.81e-39 142
MsaG021073 MtrunA17_Chr1g0167351 80.000 80 16 0 86 165 151 230 3.26e-38 139
MsaG021073 MtrunA17_Chr1g0187581 75.000 80 20 0 86 165 158 237 6.80e-35 129
MsaG021073 MtrunA17_Chr2g0299781 67.949 78 25 0 86 163 154 231 3.16e-29 114
MsaG021073 MtrunA17_Chr3g0135951 65.000 80 28 0 86 165 135 214 7.69e-29 112
MsaG021073 MtrunA17_Chr1g0153321 64.103 78 28 0 86 163 149 226 5.19e-28 110
MsaG021073 MtrunA17_Chr2g0318541 86.486 37 5 0 11 47 520 556 7.66e-13 67.0
MsaG021073 MtrunA17_Chr1g0197721 35.238 105 57 2 86 180 92 195 1.03e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021073 AT4G14350.2 81.250 80 15 0 86 165 160 239 8.82e-39 140
MsaG021073 AT4G14350.1 81.250 80 15 0 86 165 160 239 8.82e-39 140
MsaG021073 AT4G14350.4 81.250 80 15 0 86 165 160 239 8.82e-39 140
MsaG021073 AT4G14350.3 81.250 80 15 0 86 165 160 239 9.57e-39 140
MsaG021073 AT3G23310.1 80.000 80 16 0 86 165 161 240 1.04e-38 140
MsaG021073 AT1G03920.2 68.421 95 29 1 86 179 65 159 3.35e-35 130
MsaG021073 AT1G03920.1 68.421 95 29 1 86 179 178 272 4.33e-35 130
MsaG021073 AT1G03920.3 68.421 95 29 1 86 179 178 272 4.33e-35 130
MsaG021073 AT2G20470.3 66.316 95 31 1 86 179 165 259 7.87e-35 130
MsaG021073 AT2G20470.2 66.316 95 31 1 86 179 165 259 1.38e-34 129
MsaG021073 AT2G20470.1 66.316 95 31 1 86 179 165 259 1.38e-34 129
MsaG021073 AT1G30640.3 69.231 78 24 0 86 163 68 145 7.71e-30 115
MsaG021073 AT1G30640.2 69.231 78 24 0 86 163 161 238 9.69e-30 116
MsaG021073 AT1G30640.1 69.231 78 24 0 86 163 161 238 9.69e-30 116
MsaG021073 AT2G19400.1 49.640 139 62 2 49 179 102 240 1.56e-28 112
MsaG021073 AT4G33080.2 65.000 80 28 0 86 165 135 214 1.24e-27 109
MsaG021073 AT4G33080.1 65.000 80 28 0 86 165 135 214 1.26e-27 109
MsaG021073 AT5G09890.1 65.385 78 27 0 86 163 143 220 5.03e-27 108
MsaG021073 AT5G09890.2 65.385 78 27 0 86 163 143 220 5.11e-27 108
MsaG021073 AT5G04510.2 36.842 95 59 1 86 179 85 179 1.32e-11 63.5
MsaG021073 AT5G04510.1 36.842 95 59 1 86 179 85 179 2.08e-11 63.2
MsaG021073 AT5G04510.3 36.842 95 59 1 86 179 85 179 3.87e-11 62.0
MsaG021073 AT1G45160.1 28.649 185 110 5 20 183 634 817 8.25e-11 61.6

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAGTACATCTTGGTTAT+TGG 0.250300 4:+62465080 MsaT021073.1:CDS
TTGTGTTCATTGGGTTCTTC+AGG 0.252882 4:+62464221 MsaT021073.1:CDS
ACACGAACCTTGACTCTTAT+AGG 0.262348 4:+62462988 MsaT021073.1:CDS
GATGAAGCTAAGTTGTATAT+TGG 0.282849 4:+62464125 MsaT021073.1:CDS
GATTCTTAAGTTGTGTTCAT+TGG 0.290116 4:+62464211 MsaT021073.1:CDS
GGTGGTGACCACATGGATTC+AGG 0.293493 4:-62462828 None:intergenic
ATGAAGCTAAGTTGTATATT+GGG 0.300459 4:+62464126 MsaT021073.1:CDS
TGAACACAACTTAAGAATCT+TGG 0.356988 4:-62464207 None:intergenic
GGACATTTGTTGGTTGCCTT+GGG 0.362241 4:+62464755 MsaT021073.1:CDS
ATGTTTACGAATAGACTCTA+TGG 0.366298 4:-62464162 None:intergenic
CTTATCTCTTTATTCCGTAC+AGG 0.373870 4:+62462392 MsaT021073.1:CDS
CCTTTCCGACCTCTGGTGCT+TGG 0.384030 4:-62462501 None:intergenic
TGGACATTTGTTGGTTGCCT+TGG 0.385662 4:+62464754 MsaT021073.1:CDS
CACGAACCTTGACTCTTATA+GGG 0.392553 4:+62462989 MsaT021073.1:CDS
ATTCTTAAGTTGTGTTCATT+GGG 0.398961 4:+62464212 MsaT021073.1:CDS
TATGGAGCAGAACCATCCTT+TGG 0.428138 4:-62465143 None:intergenic
GAACACAACTTAAGAATCTT+GGG 0.431347 4:-62464206 None:intergenic
TGGAGAAATTTCTTTCCCTA+TGG 0.450330 4:+62462862 MsaT021073.1:CDS
CGAGTATTTATATCTCATAA+TGG 0.455404 4:+62464043 MsaT021073.1:CDS
TCATCATCGCTGAAGGAAAC+CGG 0.458878 4:-62462450 None:intergenic
CAACGTCATCATATCTCCAC+TGG 0.470628 4:-62464075 None:intergenic
TGCTATCAACTTCTGCAAGA+AGG 0.478344 4:-62463983 None:intergenic
TACAGCTAGAGCTGATAGTC+CGG 0.496465 4:+62462431 MsaT021073.1:CDS
GTCACCACCAGTGTTGGTTG+TGG 0.497369 4:+62462842 MsaT021073.1:CDS
GTGGTGACCACATGGATTCA+GGG 0.502810 4:-62462827 None:intergenic
AGATACCCGTGGACATTTGT+TGG 0.509868 4:+62464745 MsaT021073.1:CDS
AAGAGATAAGCTAAGAATGA+AGG 0.511539 4:-62462379 None:intergenic
TTATCTCTTTATTCCGTACA+GGG 0.516748 4:+62462393 MsaT021073.1:CDS
TAAATACTCGTCATCTTGAA+AGG 0.518616 4:-62464030 None:intergenic
GTCGCTGCTGCGGAATATCA+AGG 0.544183 4:+62465108 MsaT021073.1:CDS
CAACACTGGTGGTGACCACA+TGG 0.551919 4:-62462835 None:intergenic
GGCATCTTCATCATCGCTGA+AGG 0.569407 4:-62462457 None:intergenic
TGAAGCTAAGTTGTATATTG+GGG 0.573674 4:+62464127 MsaT021073.1:CDS
TCGGCCTTGAAAACCCTGTA+CGG 0.580392 4:-62462406 None:intergenic
AATTTCTCCACAACCAACAC+TGG 0.581758 4:-62462849 None:intergenic
TTGGATATGTCAAATCTGAA+AGG 0.592968 4:+62463958 MsaT021073.1:CDS
TATCAGCTCTAGCTGTACTT+CGG 0.599995 4:-62462425 None:intergenic
GGCAACCAACAAATGTCCAC+GGG 0.610059 4:-62464750 None:intergenic
AGCTCAAAACCAAGCACCAG+AGG 0.612912 4:+62462492 MsaT021073.1:CDS
AGGCAACCAACAAATGTCCA+CGG 0.615244 4:-62464751 None:intergenic
ATATGATGACGTTGCTGATG+CGG 0.617376 4:+62464084 MsaT021073.1:CDS
CTTATGAGTCATCCAAAGGA+TGG 0.622364 4:+62465131 MsaT021073.1:CDS
CACCAGGTGTTAGATACCCG+TGG 0.624596 4:+62464734 MsaT021073.1:intron
ATTGGACATAGTCGCTGCTG+CGG 0.624668 4:+62465098 MsaT021073.1:CDS
AAATTACCCTATAAGAGTCA+AGG 0.629943 4:-62462995 None:intergenic
GACATTTGTTGGTTGCCTTG+GGG 0.633865 4:+62464756 MsaT021073.1:CDS
AAGGCTTATGAGTCATCCAA+AGG 0.646224 4:+62465127 MsaT021073.1:CDS
TTCTCCACAACCAACACTGG+TGG 0.646868 4:-62462846 None:intergenic
AACGTCATCATATCTCCACT+GGG 0.651072 4:-62464074 None:intergenic
CATGTGGTCACCACCAGTGT+TGG 0.651332 4:+62462836 MsaT021073.1:CDS
ATAATGGAGTATCTACCCAG+TGG 0.705024 4:+62464059 MsaT021073.1:CDS
ATAAAATCCCTGAATCCATG+TGG 0.711354 4:+62462820 MsaT021073.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 62462375 62465166 62462375 ID=MsaG021073
Chr4 mRNA 62462375 62465166 62462375 ID=MsaT021073.1;Parent=MsaG021073
Chr4 exon 62462375 62462513 62462375 ID=MsaT021073.1.exon1;Parent=MsaT021073.1
Chr4 CDS 62462375 62462513 62462375 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Chr4 exon 62462793 62462883 62462793 ID=MsaT021073.1.exon2;Parent=MsaT021073.1
Chr4 CDS 62462793 62462883 62462793 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Chr4 exon 62462983 62463010 62462983 ID=MsaT021073.1.exon3;Parent=MsaT021073.1
Chr4 CDS 62462983 62463010 62462983 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Chr4 exon 62463957 62464242 62463957 ID=MsaT021073.1.exon4;Parent=MsaT021073.1
Chr4 CDS 62463957 62464242 62463957 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Chr4 exon 62464740 62464777 62464740 ID=MsaT021073.1.exon5;Parent=MsaT021073.1
Chr4 CDS 62464740 62464777 62464740 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Chr4 exon 62465080 62465166 62465080 ID=MsaT021073.1.exon6;Parent=MsaT021073.1
Chr4 CDS 62465080 62465166 62465080 ID=cds.MsaT021073.1;Parent=MsaT021073.1
Gene Sequence

>MsaT021073.1

ATGACCTTCATTCTTAGCTTATCTCTTTATTCCGTACAGGGTTTTCAAGGCCGAAGTACAGCTAGAGCTGATAGTCCGGTTTCCTTCAGCGATGATGAAGATGCCATGCCAACATCAAGCTCAAAACCAAGCACCAGAGGAAACATAGATGACTCCAACTTGCTAGATAAAATCCCTGAATCCATGTGGTCACCACCAGTGTTGGTTGTGGAGAAATTTCTTTCCCTATGGTACAACACGAACCTTGACTCTTATAGGGTTGGATATGTCAAATCTGAAAGGAACCTTCTTGCAGAAGTTGATAGCAATTGCATTGTAAAAATTTACTGTTCCTTTCAAGATGACGAGTATTTATATCTCATAATGGAGTATCTACCCAGTGGAGATATGATGACGTTGCTGATGCGGAAAGAAGTACTGACTGAAGATGAAGCTAAGTTGTATATTGGGGAGACTGTGCTATCCATAGAGTCTATTCGTAAACATAATTTATTACTATCTATTCATCCCAAGATTCTTAAGTTGTGTTCATTGGGTTCTTCAGGTGTTAGATACCCGTGGACATTTGTTGGTTGCCTTGGGGTGGAGTACATCTTGGTTATTGGACATAGTCGCTGCTGCGGAATATCAAGGCTTATGAGTCATCCAAAGGATGGTTCTGCTCCATAG
Protein sequence

>MsaT021073.1

MTFILSLSLYSVQGFQGRSTARADSPVSFSDDEDAMPTSSSKPSTRGNIDDSNLLDKIPESMWSPPVLVVEKFLSLWYNTNLDSYRVGYVKSERNLLAEVDSNCIVKIYCSFQDDEYLYLIMEYLPSGDMMTLLMRKEVLTEDEAKLYIGETVLSIESIRKHNLLLSIHPKILKLCSLGSSGVRYPWTFVGCLGVEYILVIGHSRCCGISRLMSHPKDGSAP*