AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021149

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021149 MtrunA17_Chr4g0035414 98.077 156 3 0 1 156 1 156 1.83e-108 305
MsaG021149 MtrunA17_Chr1g0155551 60.870 161 57 4 1 156 1 160 9.14e-62 187
MsaG021149 MtrunA17_Chr3g0144941 66.879 157 41 2 1 153 1 150 2.49e-59 181
MsaG021149 MtrunA17_Chr8g0378901 53.086 162 68 4 1 156 1 160 1.21e-48 154
MsaG021149 MtrunA17_Chr3g0141881 62.500 96 33 1 12 107 48 140 3.11e-35 120
MsaG021149 MtrunA17_Chr1g0194681 38.095 147 64 4 2 148 11 130 3.05e-24 92.0
MsaG021149 MtrunA17_Chr8g0341311 45.918 98 45 1 1 98 53 142 2.26e-20 82.8
MsaG021149 MtrunA17_Chr7g0225661 46.465 99 49 2 1 98 57 152 2.80e-20 82.8
MsaG021149 MtrunA17_Chr6g0456061 49.398 83 41 1 16 98 77 158 7.14e-19 79.3
MsaG021149 MtrunA17_Chr7g0220571 49.398 83 41 1 16 98 74 155 3.76e-18 77.0
MsaG021149 MtrunA17_Chr4g0054291 37.838 111 67 1 1 109 44 154 8.26e-18 75.9
MsaG021149 MtrunA17_Chr5g0400901 40.187 107 60 1 1 107 36 138 6.41e-16 70.5
MsaG021149 MtrunA17_Chr3g0139301 36.111 108 65 1 6 109 98 205 2.76e-13 65.5
MsaG021149 MtrunA17_Chr7g0269951 45.631 103 47 1 7 109 8 101 6.16e-12 59.7
MsaG021149 MtrunA17_Chr1g0152241 45.455 66 36 0 29 94 59 124 6.13e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021149 AT4G34590.1 62.143 140 45 4 24 156 21 159 1.34e-49 157
MsaG021149 AT1G75390.1 55.128 156 64 3 2 151 9 164 6.47e-48 153
MsaG021149 AT2G18160.1 50.345 145 56 4 27 156 28 171 1.21e-39 132
MsaG021149 AT1G75390.2 61.856 97 33 2 2 94 9 105 1.77e-33 115
MsaG021149 AT3G62420.1 44.615 130 67 1 25 154 20 144 1.35e-28 103
MsaG021149 AT1G13600.1 48.235 85 42 1 16 98 70 154 5.77e-19 79.7
MsaG021149 AT2G04038.1 48.837 86 41 1 16 98 57 142 9.72e-19 78.6
MsaG021149 AT5G15830.1 46.591 88 47 0 26 113 70 157 1.21e-18 78.6
MsaG021149 AT3G30530.1 52.055 73 35 0 26 98 77 149 2.77e-18 77.4
MsaG021149 AT5G38800.1 47.500 80 42 0 19 98 61 140 6.78e-17 73.6
MsaG021149 AT5G49450.1 38.053 113 50 3 1 110 1 96 5.76e-13 62.8
MsaG021149 AT4G37730.1 42.500 80 39 1 26 98 193 272 3.58e-12 63.2
MsaG021149 AT1G68880.1 43.284 67 38 0 28 94 45 111 4.81e-11 57.8
MsaG021149 AT2G22850.1 39.286 84 51 0 26 109 125 208 6.31e-11 58.9
MsaG021149 AT2G22850.2 39.286 84 51 0 26 109 125 208 6.31e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATATGAACCAGCCTATTA+TGG 0.236275 4:+63254934 MsaT021149.1:CDS
AGGTTCAGAAGAAGATTTAC+AGG 0.264553 4:+63254562 MsaT021149.1:CDS
AACTCCATTGCTTGAATTCA+AGG 0.317165 4:-63254843 None:intergenic
TCATCAAGTGGAACATCTTC+AGG 0.318453 4:+63254518 MsaT021149.1:CDS
AGAAGATTTACAGGCTTTGA+TGG 0.350638 4:+63254571 MsaT021149.1:CDS
ATATCTGCAGAAGCCATAAT+AGG 0.384326 4:-63254947 None:intergenic
GCGAATCGGCAAGGCGGTCT+AGG 0.434095 4:+63254627 MsaT021149.1:CDS
AGCTGAAAATTCAGTGTTGA+GGG 0.457089 4:+63254766 MsaT021149.1:CDS
TTCAGTGTTGAGGGCTCAGA+TGG 0.472933 4:+63254775 MsaT021149.1:CDS
TGGATCAGAGAAAGAGGAAA+AGG 0.477494 4:+63254591 MsaT021149.1:CDS
GATGATATCGAACCGCGAAT+CGG 0.489986 4:+63254613 MsaT021149.1:CDS
GAATCTTTGAATGAAATTGT+TGG 0.493743 4:+63254818 MsaT021149.1:CDS
AGATGGGTGAGTTAAGCAAC+AGG 0.493753 4:+63254792 MsaT021149.1:CDS
TAGACCGCCTTGCCGATTCG+CGG 0.495356 4:-63254625 None:intergenic
CGGCAAGGCGGTCTAGGATG+AGG 0.519007 4:+63254633 MsaT021149.1:CDS
TTGAAGAAGAAACCATTACT+TGG 0.526748 4:-63254893 None:intergenic
CTCATCATGGCTTCATCAAG+TGG 0.531339 4:+63254506 None:intergenic
TGTCACAAGTTGCTAAGCTA+AGG 0.534668 4:+63254681 MsaT021149.1:CDS
CTTTGATGGATCAGAGAAAG+AGG 0.544044 4:+63254585 MsaT021149.1:CDS
CTGCAGAAGCCATAATAGGC+TGG 0.561236 4:-63254943 None:intergenic
AAGCTGAAAATTCAGTGTTG+AGG 0.592611 4:+63254765 MsaT021149.1:CDS
GGGTGAGTTAAGCAACAGGT+TGG 0.612598 4:+63254796 MsaT021149.1:CDS
TATCGAACCGCGAATCGGCA+AGG 0.622907 4:+63254618 MsaT021149.1:CDS
TCATCTCTACTACAAAACTC+AGG 0.649209 4:+63254542 MsaT021149.1:CDS
TCAGTGTTGAGGGCTCAGAT+GGG 0.662073 4:+63254776 MsaT021149.1:CDS
GGTGCCTTGAATTCAAGCAA+TGG 0.668976 4:+63254839 MsaT021149.1:CDS
CGAACCGCGAATCGGCAAGG+CGG 0.745432 4:+63254621 MsaT021149.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 63254512 63254982 63254512 ID=MsaG021149
Chr4 mRNA 63254512 63254982 63254512 ID=MsaT021149.1;Parent=MsaG021149
Chr4 exon 63254512 63254982 63254512 ID=MsaT021149.1.exon1;Parent=MsaT021149.1
Chr4 CDS 63254512 63254982 63254512 ID=cds.MsaT021149.1;Parent=MsaT021149.1
Gene Sequence

>MsaT021149.1

ATGGCTTCATCAAGTGGAACATCTTCAGGTTCATCTCTACTACAAAACTCAGGTTCAGAAGAAGATTTACAGGCTTTGATGGATCAGAGAAAGAGGAAAAGGATGATATCGAACCGCGAATCGGCAAGGCGGTCTAGGATGAGGAAACAGAAACACTTAGATGATCTTGTGTCACAAGTTGCTAAGCTAAGGAAAGAGAATCAAGAAATACTCACAAGTGTTAACATCACCACTCAAAAGTATTTGAGTGTTGAAGCTGAAAATTCAGTGTTGAGGGCTCAGATGGGTGAGTTAAGCAACAGGTTGGAATCTTTGAATGAAATTGTTGGTGCCTTGAATTCAAGCAATGGAGTTTTTGGAGCATCAAATGCTTTTGTTGAGCCAAGTAATGGTTTCTTCTTCAACTCTTTGAATAATATGTCTTATATGAACCAGCCTATTATGGCTTCTGCAGATATACTGCAGTACTGA
Protein sequence

>MsaT021149.1

MASSSGTSSGSSLLQNSGSEEDLQALMDQRKRKRMISNRESARRSRMRKQKHLDDLVSQVAKLRKENQEILTSVNITTQKYLSVEAENSVLRAQMGELSNRLESLNEIVGALNSSNGVFGASNAFVEPSNGFFFNSLNNMSYMNQPIMASADILQY*