AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021167

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021167 MtrunA17_Chr4g0035651 96.861 223 6 1 1 222 1 223 1.23e-161 447
MsaG021167 MtrunA17_Chr3g0145061 60.094 213 68 7 1 198 1 211 7.21e-77 232
MsaG021167 MtrunA17_Chr1g0155811 45.815 227 89 4 1 208 1 212 2.34e-57 184
MsaG021167 MtrunA17_Chr3g0142221 47.191 178 58 3 1 177 1 143 2.00e-51 167
MsaG021167 MtrunA17_Chr2g0314671 50.794 126 58 3 1 126 1 122 4.83e-36 129
MsaG021167 MtrunA17_Chr4g0033641 50.000 110 54 1 1 110 1 109 1.40e-34 124
MsaG021167 MtrunA17_Chr2g0329511 48.182 110 56 1 1 110 1 109 8.25e-32 117
MsaG021167 MtrunA17_Chr5g0405151 37.324 142 78 4 1 135 1 138 6.23e-23 92.4
MsaG021167 MtrunA17_Chr1g0150471 43.299 97 45 1 5 101 6 92 7.12e-20 87.0
MsaG021167 MtrunA17_Chr3g0136701 39.175 97 49 1 5 101 6 92 9.45e-17 78.2
MsaG021167 MtrunA17_Chr4g0072871 40.860 93 45 1 5 97 22 104 1.83e-16 78.2
MsaG021167 MtrunA17_Chr7g0220721 35.043 117 63 2 5 121 26 129 2.38e-15 75.1
MsaG021167 MtrunA17_Chr5g0429341 36.082 97 52 1 1 97 1 87 5.42e-14 70.9
MsaG021167 MtrunA17_Chr3g0120711 35.052 97 52 2 1 97 1 86 1.98e-11 63.5
MsaG021167 MtrunA17_Chr7g0268691 32.653 98 55 2 5 102 20 106 6.76e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021167 AT4G39070.1 53.500 200 69 4 1 176 1 200 2.10e-58 186
MsaG021167 AT1G75540.1 35.625 320 135 9 1 250 1 319 2.11e-54 179
MsaG021167 AT1G06040.2 46.715 137 66 2 1 137 1 130 9.04e-37 128
MsaG021167 AT1G78600.1 51.724 116 55 1 1 116 1 115 2.33e-36 131
MsaG021167 AT1G78600.2 51.724 116 55 1 1 116 1 115 3.49e-36 131
MsaG021167 AT1G06040.1 46.715 137 66 2 1 137 1 130 1.01e-35 128
MsaG021167 AT2G31380.1 51.327 113 54 1 1 113 1 112 1.40e-33 122
MsaG021167 AT4G10240.1 44.138 145 67 4 1 135 1 141 1.31e-30 112
MsaG021167 AT4G38960.1 43.810 105 55 2 1 105 1 101 2.28e-23 94.0
MsaG021167 AT4G38960.6 43.810 105 55 2 1 105 1 101 2.28e-23 94.0
MsaG021167 AT2G21320.1 43.810 105 55 2 1 105 1 101 4.91e-22 90.1
MsaG021167 AT2G24790.2 41.237 97 47 1 5 101 8 94 4.58e-19 83.6
MsaG021167 AT2G24790.1 41.237 97 47 1 5 101 8 94 1.24e-18 83.6
MsaG021167 AT5G24930.1 37.113 97 51 1 5 101 6 92 2.65e-16 77.8
MsaG021167 AT5G15850.1 32.520 123 70 2 5 127 12 121 9.27e-16 76.3
MsaG021167 AT4G38960.3 31.081 148 55 3 1 105 1 144 2.14e-15 73.6
MsaG021167 AT5G15840.2 33.333 123 70 2 5 127 20 130 6.49e-15 73.2
MsaG021167 AT5G15840.1 33.333 123 70 2 5 127 20 130 9.22e-15 73.6
MsaG021167 AT3G02380.1 36.559 93 49 1 5 97 16 98 1.38e-14 72.8
MsaG021167 AT2G47890.2 40.244 82 39 1 5 86 13 84 1.96e-12 65.5
MsaG021167 AT1G28050.1 35.644 101 55 1 2 102 6 96 2.05e-12 67.0
MsaG021167 AT2G47890.3 40.244 82 39 1 5 86 13 84 2.90e-12 65.9
MsaG021167 AT2G47890.1 40.244 82 39 1 5 86 13 84 4.51e-12 65.5
MsaG021167 AT5G48250.1 34.694 98 53 2 1 97 1 88 5.34e-12 65.5
MsaG021167 AT2G33500.2 34.000 100 56 1 3 102 10 99 8.20e-12 65.1
MsaG021167 AT2G33500.1 34.000 100 56 1 3 102 10 99 8.38e-12 65.1
MsaG021167 AT5G57660.1 34.646 127 60 4 5 122 22 134 1.19e-11 64.3
MsaG021167 AT3G07650.3 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
MsaG021167 AT3G07650.2 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
MsaG021167 AT3G07650.4 36.047 86 45 1 1 86 1 76 6.07e-11 62.4
MsaG021167 AT3G07650.1 36.047 86 45 1 1 86 1 76 6.07e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTACAGAAGACGTTATT+AGG 0.294008 4:-63493298 None:intergenic
AATTGTTTCAATCAAGTATT+CGG 0.312961 4:-63493233 None:intergenic
ATCACAGAGAGGGGTGTCTT+TGG 0.337373 4:-63492669 None:intergenic
CTTGAAAATGAACTTGGTCT+TGG 0.353565 4:-63493136 None:intergenic
CCCCGTCTAAAGAAGCAATC+TGG 0.356882 4:-63493549 None:intergenic
TGTTTATAACTTGAAGATCT+TGG 0.361700 4:-63493462 None:intergenic
CTGAGCCATTGGAAGTTGTT+GGG 0.365142 4:-63493096 None:intergenic
TGGTAAGTTTGTTGACTTCA+TGG 0.370579 4:-63493012 None:intergenic
TCTGAGCCATTGGAAGTTGT+TGG 0.394596 4:-63493097 None:intergenic
CCCAGATTGCTTCTTTAGAC+GGG 0.396501 4:+63493548 MsaT021167.1:CDS
TACTTGATTGAAACAATTCC+TGG 0.400109 4:+63493238 MsaT021167.1:CDS
AAGACAGAGCAATACTTTGC+AGG 0.405273 4:+63492972 MsaT021167.1:CDS
CAGAGAGCTGCTTCATCTGC+AGG 0.413650 4:-63492566 None:intergenic
CCCCAGATTGCTTCTTTAGA+CGG 0.429154 4:+63493547 MsaT021167.1:CDS
GTTCTCAAAGTTCTTTCAAA+GGG 0.440989 4:+63493173 MsaT021167.1:CDS
GCGTAAAAGAAGCATCAAAA+AGG 0.446259 4:-63493276 None:intergenic
AAGTTATAAACATGTCTGAC+TGG 0.451524 4:+63493473 MsaT021167.1:CDS
AACTTACCAAGCAACACAAC+AGG 0.456566 4:+63493026 MsaT021167.1:CDS
CAACACACTAAAGCTGTTGA+AGG 0.461687 4:+63493583 MsaT021167.1:CDS
TGAACTTGGTCTTGGACTTG+AGG 0.462812 4:-63493128 None:intergenic
GTGTTTAGCAGAGACCTTGT+TGG 0.467471 4:-63492618 None:intergenic
CAGAGACCTTGTTGGCATAG+TGG 0.468511 4:-63492610 None:intergenic
GCCTAATAACGTCTTCTGTA+AGG 0.477486 4:+63493297 MsaT021167.1:CDS
CCCGTCTAAAGAAGCAATCT+GGG 0.479125 4:-63493548 None:intergenic
CAAGTATTCGGAAATGCTAC+TGG 0.481122 4:-63493221 None:intergenic
CCTCTACATTGTTTGATGAA+TGG 0.482437 4:-63493631 None:intergenic
GGTTCTTAGTTCTGAGCCAT+TGG 0.490954 4:-63493107 None:intergenic
AGAGGGGTGTCTTTGGAAGT+GGG 0.493908 4:-63492662 None:intergenic
GAGAGGGGTGTCTTTGGAAG+TGG 0.504462 4:-63492663 None:intergenic
TGTTCTCAAAGTTCTTTCAA+AGG 0.505912 4:+63493172 MsaT021167.1:CDS
ATGAATGGAGGACTAGTTGA+AGG 0.506506 4:-63493616 None:intergenic
AAGTTCTTTCAAAGGGAACA+TGG 0.523905 4:+63493180 MsaT021167.1:CDS
AAGAAACCTGTTGTGTTGCT+TGG 0.533532 4:-63493032 None:intergenic
CACAACAGGTTTCTTCTCAC+AGG 0.544493 4:+63493040 MsaT021167.1:CDS
AACAATTCCTGGTTACTGCA+TGG 0.545989 4:+63493249 MsaT021167.1:CDS
TTCATCTGCAGGGCAGAACA+AGG 0.547884 4:-63492555 None:intergenic
CTTCTCACAGGTGTAAAGAT+CGG 0.556317 4:+63493052 MsaT021167.1:CDS
AGACAGAGCAATACTTTGCA+GGG 0.562722 4:+63492973 MsaT021167.1:CDS
TCAAACCCAACAACTTCCAA+TGG 0.565279 4:+63493091 MsaT021167.1:CDS
AGGTCTTCCATGCAGTAACC+AGG 0.571610 4:-63493256 None:intergenic
AGTTTGTTGACTTCATGGAT+AGG 0.572223 4:-63493007 None:intergenic
CTACATTGTTTGATGAATGG+AGG 0.578479 4:-63493628 None:intergenic
CCAGATTGCTTCTTTAGACG+GGG 0.585647 4:+63493549 MsaT021167.1:CDS
AGAGAGCTGCTTCATCTGCA+GGG 0.589609 4:-63492565 None:intergenic
TCGAATTAGCACTAAGCTGA+GGG 0.591073 4:-63493519 None:intergenic
GGGAATCTAACTTGAGATTG+TGG 0.592001 4:-63493499 None:intergenic
CAATATCCACTATGCCAACA+AGG 0.603596 4:+63492604 MsaT021167.1:CDS
TTCGAATTAGCACTAAGCTG+AGG 0.605701 4:-63493520 None:intergenic
CCTTGCAGATATCACAGAGA+GGG 0.606602 4:-63492679 None:intergenic
CCCTCTCTGTGATATCTGCA+AGG 0.608181 4:+63492679 MsaT021167.1:CDS
GTTTGTGACATGCATCACAC+TGG 0.618408 4:-63492523 None:intergenic
TGATGCATGTCACAAACAAG+AGG 0.622158 4:+63492529 MsaT021167.1:CDS
GAGGGGTGTCTTTGGAAGTG+GGG 0.627649 4:-63492661 None:intergenic
AGTTATAAACATGTCTGACT+GGG 0.637692 4:+63493474 MsaT021167.1:CDS
CCATTCATCAAACAATGTAG+AGG 0.654007 4:+63493631 MsaT021167.1:CDS
ATCTGCAGGGCAGAACAAGG+AGG 0.684576 4:-63492552 None:intergenic
CTTGCAGATATCACAGAGAG+GGG 0.687405 4:-63492678 None:intergenic
CCGTCTAAAGAAGCAATCTG+GGG 0.713354 4:-63493547 None:intergenic
ACCTTGCAGATATCACAGAG+AGG 0.718667 4:-63492680 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 63492515 63493663 63492515 ID=MsaG021167
Chr4 mRNA 63492515 63493663 63492515 ID=MsaT021167.1;Parent=MsaG021167
Chr4 exon 63492515 63492700 63492515 ID=MsaT021167.1.exon1;Parent=MsaT021167.1
Chr4 CDS 63492515 63492700 63492515 ID=cds.MsaT021167.1;Parent=MsaT021167.1
Chr4 exon 63492944 63493318 63492944 ID=MsaT021167.1.exon2;Parent=MsaT021167.1
Chr4 CDS 63492944 63493318 63492944 ID=cds.MsaT021167.1;Parent=MsaT021167.1
Chr4 exon 63493445 63493663 63493445 ID=MsaT021167.1.exon3;Parent=MsaT021167.1
Chr4 CDS 63493445 63493663 63493445 ID=cds.MsaT021167.1;Parent=MsaT021167.1
Gene Sequence

>MsaT021167.1

ATGAAGATCCAGTGTGATGCATGTCACAAACAAGAGGCCTCCTTGTTCTGCCCTGCAGATGAAGCAGCTCTCTGCAATCAATGTGATCGCAATATCCACTATGCCAACAAGGTCTCTGCTAAACACAAACGCTTCACTCTTCATCACCCCACTTCCAAAGACACCCCTCTCTGTGATATCTGCAAGGAGAGGCGTGCATATCTATTTTGCAAAGAAGACAGAGCAATACTTTGCAGGGAATGTGACATTCCTATCCATGAAGTCAACAAACTTACCAAGCAACACAACAGGTTTCTTCTCACAGGTGTAAAGATCGGTGCTTCTTCTTCTTATTCAAACCCAACAACTTCCAATGGCTCAGAACTAAGAACCTCAAGTCCAAGACCAAGTTCATTTTCAAGTGAAAATAATAGTTGTTCTCAAAGTTCTTTCAAAGGGAACATGGCTTGTGACACAGTTTCAACCAGTAGCATTTCCGAATACTTGATTGAAACAATTCCTGGTTACTGCATGGAAGACCTTTTTGATGCTTCTTTTACGCCTAATAACGTCTTCTGTAAGGATTATTATGAACAGAACCAAGATCTTCAAGTTATAAACATGTCTGACTGGGTACCACAATCTCAAGTTAGATTCCCTCAGCTTAGTGCTAATTCGAATGTTCCCCAGATTGCTTCTTTAGACGGGGTTATGAAAATGCAACACACTAAAGCTGTTGAAGGATACAAAGTTCCTTCAACTAGTCCTCCATTCATCAAACAATGTAGAGGCACTCGTTAA
Protein sequence

>MsaT021167.1

MKIQCDACHKQEASLFCPADEAALCNQCDRNIHYANKVSAKHKRFTLHHPTSKDTPLCDICKERRAYLFCKEDRAILCRECDIPIHEVNKLTKQHNRFLLTGVKIGASSSYSNPTTSNGSELRTSSPRPSSFSSENNSCSQSSFKGNMACDTVSTSSISEYLIETIPGYCMEDLFDASFTPNNVFCKDYYEQNQDLQVINMSDWVPQSQVRFPQLSANSNVPQIASLDGVMKMQHTKAVEGYKVPSTSPPFIKQCRGTR*