AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021184


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021184 MtrunA17_Chr4g0035891 96.959 296 8 1 1 296 1 295 0.0 596
MsaG021184 MtrunA17_Chr3g0145121 53.292 319 107 8 1 296 1 300 1.42e-97 289
MsaG021184 MtrunA17_Chr8g0372291 39.159 309 138 10 1 281 1 287 9.13e-52 171
MsaG021184 MtrunA17_Chr5g0404981 40.282 355 137 11 1 296 51 389 2.70e-50 171
MsaG021184 MtrunA17_Chr3g0140331 38.406 276 120 9 36 281 67 322 4.47e-45 156
MsaG021184 MtrunA17_Chr8g0340401 38.869 283 113 9 39 281 33 295 2.26e-43 151
MsaG021184 MtrunA17_Chr1g0146231 34.921 315 141 12 15 281 5 303 1.09e-42 149
MsaG021184 MtrunA17_Chr1g0199671 75.714 70 17 0 207 276 237 306 1.33e-34 128
MsaG021184 MtrunA17_Chr6g0461591 81.538 65 12 0 212 276 192 256 2.08e-34 125
MsaG021184 MtrunA17_Chr7g0253101 80.000 65 13 0 212 276 188 252 2.24e-33 123
MsaG021184 MtrunA17_Chr1g0213161 67.532 77 25 0 212 288 152 228 2.84e-33 122
MsaG021184 MtrunA17_Chr1g0169431 76.471 68 16 0 212 279 172 239 8.18e-33 121
MsaG021184 MtrunA17_Chr2g0326771 62.791 86 30 1 191 276 197 280 2.54e-32 121
MsaG021184 MtrunA17_Chr4g0028961 73.134 67 18 0 212 278 232 298 3.70e-32 121
MsaG021184 MtrunA17_Chr5g0395391 68.571 70 22 0 212 281 270 339 1.40e-29 114
MsaG021184 MtrunA17_Chr5g0395391 59.036 83 28 1 199 275 149 231 2.99e-26 105
MsaG021184 MtrunA17_Chr7g0232671 57.143 63 27 0 211 273 186 248 1.72e-20 88.6
MsaG021184 MtrunA17_Chr7g0232701 53.968 63 29 0 211 273 72 134 7.17e-19 81.3
MsaG021184 MtrunA17_Chr7g0232681 31.868 182 107 5 105 273 16 193 4.94e-18 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021184 AT4G36240.1 47.082 257 90 10 40 282 10 234 1.40e-56 182
MsaG021184 AT5G66320.2 35.838 346 139 10 8 291 5 329 2.41e-51 172
MsaG021184 AT5G66320.1 35.838 346 139 10 8 291 5 329 2.41e-51 172
MsaG021184 AT4G34680.1 41.034 290 114 11 6 283 7 251 2.03e-49 165
MsaG021184 AT4G34680.2 41.034 290 114 11 6 283 7 251 2.03e-49 165
MsaG021184 AT5G25830.1 38.047 297 108 12 40 281 13 288 1.49e-44 154
MsaG021184 AT3G60530.1 47.778 180 73 5 112 283 63 229 2.33e-43 149
MsaG021184 AT4G32890.1 36.331 278 115 11 39 281 16 266 4.85e-43 150
MsaG021184 AT2G45050.1 44.845 194 73 4 112 281 65 248 5.01e-43 148
MsaG021184 AT3G51080.1 69.231 91 22 2 212 296 221 311 2.52e-38 137
MsaG021184 AT3G24050.1 64.706 85 29 1 197 280 178 262 1.05e-33 124
MsaG021184 AT3G54810.1 75.000 68 17 0 212 279 229 296 3.82e-33 124
MsaG021184 AT3G54810.2 75.000 68 17 0 212 279 229 296 3.82e-33 124
MsaG021184 AT1G08010.2 72.464 69 19 0 212 280 220 288 3.59e-32 121
MsaG021184 AT1G08010.1 72.464 69 19 0 212 280 220 288 3.59e-32 121
MsaG021184 AT1G08010.3 72.464 69 19 0 212 280 220 288 3.59e-32 121
MsaG021184 AT1G08000.2 71.014 69 20 0 212 280 218 286 2.77e-30 116
MsaG021184 AT1G08000.1 71.014 69 20 0 212 280 218 286 2.77e-30 116
MsaG021184 AT3G45170.1 60.274 73 29 0 214 286 117 189 9.60e-27 104
MsaG021184 AT2G28340.1 63.077 65 24 0 214 278 193 257 1.64e-24 100

Find 89 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTGTCTTGGTTGTTCT+AGG 0.195513 4:-63693812 None:intergenic
AGCCCCACCCGTTTGGGCTT+CGG 0.226880 4:-63693900 None:intergenic
GCCCCACCCGTTTGGGCTTC+GGG 0.232140 4:-63693899 None:intergenic
GGCCGATGATGTTGCGGATT+TGG 0.241470 4:+63693655 MsaT021184.1:intron
GAACAGCCACCGTAAGGTTT+TGG 0.270098 4:+63694096 MsaT021184.1:CDS
GAGGTGGATGAACCGGTTTC+CGG 0.310815 4:+63694136 MsaT021184.1:CDS
GGTTCGGCCCGGGCCTGAAC+TGG 0.312951 4:-63693731 None:intergenic
TGCCGGTGGTTTAGCAGTTC+CGG 0.331211 4:+63693377 MsaT021184.1:CDS
GAAACTGAGCCCCACCCGTT+TGG 0.331959 4:-63693907 None:intergenic
TCTTTGCTGAAAAGAGTATA+GGG 0.337151 4:-63693862 None:intergenic
TCTCTTCACAAATTGTTTGT+TGG 0.346081 4:-63693098 None:intergenic
AAACTGAGCCCCACCCGTTT+GGG 0.350163 4:-63693906 None:intergenic
CTTCTTCTTCTTCCTCCTCC+TGG 0.352825 4:-63693239 None:intergenic
CTCTCTATTGTATCCAGTTC+AGG 0.364580 4:+63693718 MsaT021184.1:CDS
AACCGGTTTCCGGTCTGAAC+CGG 0.370991 4:+63694146 MsaT021184.1:CDS
GCATTGGTTGGGCCCGGTCT+AGG 0.372639 4:-63693758 None:intergenic
ACAGTTGGGCCTTCTTGTCT+TGG 0.379924 4:-63693822 None:intergenic
GTTGGGCCCGGTCTAGGTTC+CGG 0.399228 4:-63693752 None:intergenic
AGTTCAGGCCCGGGCCGAAC+CGG 0.407384 4:+63693733 MsaT021184.1:CDS
GTCTAGGTTCCGGTTCGGCC+CGG 0.414385 4:-63693742 None:intergenic
GGCCGAACCGGAACCTAGAC+CGG 0.414860 4:+63693745 MsaT021184.1:CDS
TTCTTTGCTGAAAAGAGTAT+AGG 0.416988 4:-63693863 None:intergenic
AACGCAAACAACGTTGTTGT+CGG 0.417578 4:+63693132 MsaT021184.1:CDS
TTTGGAAATGAGGCGGAGGA+AGG 0.432787 4:+63694114 MsaT021184.1:CDS
TATGTAACGCTTGTGGTGTT+CGG 0.433641 4:+63694002 MsaT021184.1:CDS
GAATTTCACCGGAAAACGTC+GGG 0.435549 4:-63694069 None:intergenic
GAATTTCAACATGGTTCTGT+TGG 0.444517 4:+63693198 MsaT021184.1:CDS
TATTGTATCCAGTTCAGGCC+CGG 0.446736 4:+63693723 MsaT021184.1:CDS
GGAATGGGTGTCTCACTTTG+TGG 0.449579 4:+63693676 MsaT021184.1:CDS
AATGAGGCGGAGGAAGGAGG+TGG 0.451531 4:+63694120 MsaT021184.1:CDS
ATCCGGTTCAGACCGGAAAC+CGG 0.452815 4:-63694148 None:intergenic
GAGGAAGGAGGTGGATGAAC+CGG 0.452883 4:+63694129 MsaT021184.1:CDS
TCTAGGTTCCGGTTCGGCCC+GGG 0.468440 4:-63693741 None:intergenic
ATAGATTCTGTCCTCAAGCT+TGG 0.468461 4:-63693072 None:intergenic
GCCTCATTTCCAAAACCTTA+CGG 0.469337 4:-63694105 None:intergenic
TTCTGCGGATTATAGAGAGC+TGG 0.472534 4:-63693787 None:intergenic
CGGTCTGAACCGGATTCAAA+TGG 0.475660 4:+63694156 MsaT021184.1:CDS
ATGATGTTGCGGATTTGGAA+TGG 0.479723 4:+63693660 MsaT021184.1:CDS
AACTCGTGACCATTTGAATC+CGG 0.481371 4:-63694165 None:intergenic
CAGTGGAGAGCTGGTCCGCT+TGG 0.483307 4:+63693968 MsaT021184.1:CDS
GGAGTCAGACTCGCCGTCTC+CGG 0.485968 4:-63693349 None:intergenic
TGTGGTGTTCGGTTTAAGTC+CGG 0.492368 4:+63694013 MsaT021184.1:CDS
GGATACAATAGAGAGAGTTC+TGG 0.493646 4:-63693710 None:intergenic
GCCCGAAGCCCAAACGGGTG+GGG 0.493923 4:+63693898 MsaT021184.1:CDS
AAAATCCACACGTTACAGTT+GGG 0.498357 4:-63693836 None:intergenic
TGATGTTGCGGATTTGGAAT+GGG 0.501793 4:+63693661 MsaT021184.1:CDS
GGAAATGAGGCGGAGGAAGG+AGG 0.505698 4:+63694117 MsaT021184.1:CDS
GCCCGGTCTAGGTTCCGGTT+CGG 0.510484 4:-63693747 None:intergenic
AGACTCGCCGTCTCCGGCAC+CGG 0.510633 4:-63693343 None:intergenic
AGCTGGGAATAAGCATTGGT+TGG 0.510903 4:-63693770 None:intergenic
TTCTCAGGCCGATGATGTTG+CGG 0.519029 4:+63693649 MsaT021184.1:intron
AAAAGAAATTGATGTTTACC+AGG 0.530934 4:+63693221 MsaT021184.1:CDS
GCTGGGAATAAGCATTGGTT+GGG 0.531676 4:-63693769 None:intergenic
GAAAACGTCGGGCTGCATGC+AGG 0.532133 4:-63694058 None:intergenic
AAAACGCCACAGTGGAGAGC+TGG 0.532352 4:+63693959 MsaT021184.1:CDS
AATTGATGTTTACCAGGAGG+AGG 0.533216 4:+63693227 MsaT021184.1:CDS
TTCCAAATCCGCAACATCAT+CGG 0.540001 4:-63693657 None:intergenic
AGCCCGAAGCCCAAACGGGT+GGG 0.541657 4:+63693897 MsaT021184.1:CDS
GAATAAGCATTGGTTGGGCC+CGG 0.542060 4:-63693764 None:intergenic
ATTGTATCCAGTTCAGGCCC+GGG 0.543140 4:+63693724 MsaT021184.1:CDS
CTGCATGCAGGTCTATACTC+AGG 0.544535 4:-63694046 None:intergenic
AGAGAGCTGGGAATAAGCAT+TGG 0.549150 4:-63693774 None:intergenic
GCCGAACCGGAACCTAGACC+GGG 0.552879 4:+63693746 MsaT021184.1:CDS
TCTGCGGATTATAGAGAGCT+GGG 0.553907 4:-63693786 None:intergenic
CATTTGAATCCGGTTCAGAC+CGG 0.555998 4:-63694155 None:intergenic
GCTGTTCGAATGAATTTCAC+CGG 0.556294 4:-63694080 None:intergenic
AAGCCCGAAGCCCAAACGGG+TGG 0.557944 4:+63693896 MsaT021184.1:CDS
TCCGGCACCGGAAAATGTTG+TGG 0.574032 4:-63693331 None:intergenic
TCATTCGAACAGCCACCGTA+AGG 0.576237 4:+63694090 MsaT021184.1:CDS
ATACTCAGGGAAAAGCCGAC+CGG 0.581357 4:-63694032 None:intergenic
CTAGAACAACCAAGACAAGA+AGG 0.581886 4:+63693813 MsaT021184.1:CDS
ACCATGTTGAAATTCACCGT+TGG 0.582797 4:-63693190 None:intergenic
CACCGGAACTGCTAAACCAC+CGG 0.593758 4:-63693379 None:intergenic
TGGTTGTTCTAGGCTTTCTG+CGG 0.603627 4:-63693802 None:intergenic
TGAATTTCACCGGAAAACGT+CGG 0.605768 4:-63694070 None:intergenic
AAACATGGAAGTTGTTGTAG+CGG 0.614309 4:+63693038 None:intergenic
TGCATGCAGGTCTATACTCA+GGG 0.615040 4:-63694045 None:intergenic
TCGTTCATGTTCAGAACCTG+AGG 0.628083 4:-63693283 None:intergenic
TTCATGTTCAGAACCTGAGG+TGG 0.629594 4:-63693280 None:intergenic
GTGTTCGGTTTAAGTCCGGT+CGG 0.631814 4:+63694017 MsaT021184.1:CDS
TCCAACGGTGAATTTCAACA+TGG 0.652718 4:+63693189 MsaT021184.1:CDS
GAAGGCCCAACTGTAACGTG+TGG 0.654146 4:+63693831 MsaT021184.1:CDS
AAAACACTATGTAACGCTTG+TGG 0.656575 4:+63693995 MsaT021184.1:CDS
AGAAATTGATGTTTACCAGG+AGG 0.667546 4:+63693224 MsaT021184.1:CDS
AAGCGTTGAAACCAAGCTTG+AGG 0.681951 4:+63693061 MsaT021184.1:CDS
TCATTTCCAAAACCTTACGG+TGG 0.687073 4:-63694102 None:intergenic
AGCGGACCAGCTCTCCACTG+TGG 0.697012 4:-63693965 None:intergenic
ATGTTCAGAAAACGCCACAG+TGG 0.715931 4:+63693951 MsaT021184.1:CDS
GTGGGGCTCAGTTTCAACGA+CGG 0.724625 4:+63693915 MsaT021184.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 63693042 63694189 63693042 ID=MsaG021184
Chr4 mRNA 63693042 63694189 63693042 ID=MsaT021184.1;Parent=MsaG021184
Chr4 exon 63693042 63693398 63693042 ID=MsaT021184.1.exon1;Parent=MsaT021184.1
Chr4 CDS 63693042 63693398 63693042 ID=cds.MsaT021184.1;Parent=MsaT021184.1
Chr4 exon 63693656 63694189 63693656 ID=MsaT021184.1.exon2;Parent=MsaT021184.1
Chr4 CDS 63693656 63694189 63693656 ID=cds.MsaT021184.1;Parent=MsaT021184.1
Gene Sequence

>MsaT021184.1

ATGGAAGTTGTTGTAGCGGAAGCGTTGAAACCAAGCTTGAGGACAGAATCTATTTTCCAACAAACAATTTGTGAAGAGATTTTGTGTTTGAACGCAAACAACGTTGTTGTCGGAGAAGATTTTTCTGTCGACGACTTGCTTGATTTTTCCAACGGTGAATTTCAACATGGTTCTGTTGGAAAAGAAATTGATGTTTACCAGGAGGAGGAAGAAGAAGAAGAACATGAAAAAAACAGCACCACCTCAGGTTCTGAACATGAACGAACAGAAGATGACAACAATTCAAATTCCACAACATTTTCCGGTGCCGGAGACGGCGAGTCTGACTCCATTTTTGCCGGTGGTTTAGCAGTTCCGGCCGATGATGTTGCGGATTTGGAATGGGTGTCTCACTTTGTGGACGATTCTGTACCAGAACTCTCTCTATTGTATCCAGTTCAGGCCCGGGCCGAACCGGAACCTAGACCGGGCCCAACCAATGCTTATTCCCAGCTCTCTATAATCCGCAGAAAGCCTAGAACAACCAAGACAAGAAGGCCCAACTGTAACGTGTGGATTTTTAACCCTATACTCTTTTCAGCAAAGAAGCAGAGGAAAAAGCCCGAAGCCCAAACGGGTGGGGCTCAGTTTCAACGACGGTGCAGCCACTGCCATGTTCAGAAAACGCCACAGTGGAGAGCTGGTCCGCTTGGACCGAAAACACTATGTAACGCTTGTGGTGTTCGGTTTAAGTCCGGTCGGCTTTTCCCTGAGTATAGACCTGCATGCAGCCCGACGTTTTCCGGTGAAATTCATTCGAACAGCCACCGTAAGGTTTTGGAAATGAGGCGGAGGAAGGAGGTGGATGAACCGGTTTCCGGTCTGAACCGGATTCAAATGGTCACGAGTTGA

Protein sequence

>MsaT021184.1

MEVVVAEALKPSLRTESIFQQTICEEILCLNANNVVVGEDFSVDDLLDFSNGEFQHGSVGKEIDVYQEEEEEEEHEKNSTTSGSEHERTEDDNNSNSTTFSGAGDGESDSIFAGGLAVPADDVADLEWVSHFVDDSVPELSLLYPVQARAEPEPRPGPTNAYSQLSIIRRKPRTTKTRRPNCNVWIFNPILFSAKKQRKKPEAQTGGAQFQRRCSHCHVQKTPQWRAGPLGPKTLCNACGVRFKSGRLFPEYRPACSPTFSGEIHSNSHRKVLEMRRRKEVDEPVSGLNRIQMVTS*