AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021278


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021278 MtrunA17_Chr1g0172961 78.295 258 41 5 1 245 1 256 5.45e-135 382
MsaG021278 MtrunA17_Chr4g0037041 87.050 139 16 1 1 137 1 139 1.11e-83 249
MsaG021278 MtrunA17_Chr1g0172861 44.272 323 83 11 1 274 1 275 4.15e-57 184
MsaG021278 MtrunA17_Chr1g0169521 34.967 306 133 11 20 278 28 314 2.03e-33 124
MsaG021278 MtrunA17_Chr1g0169561 34.967 306 133 11 20 278 28 314 3.18e-33 123
MsaG021278 MtrunA17_Chr7g0214511 39.200 250 104 12 42 278 71 285 6.73e-24 97.8
MsaG021278 MtrunA17_Chr1g0174871 33.333 264 149 10 31 278 31 283 8.81e-24 97.8
MsaG021278 MtrunA17_Chr1g0174861 35.047 214 112 7 23 235 25 212 2.92e-21 90.1
MsaG021278 MtrunA17_Chr1g0174001 37.255 204 102 11 90 278 1 193 2.11e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021278 AT2G45120.1 30.233 129 79 1 19 136 180 308 2.14e-11 63.5

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGATCATGAACATCTTAA+TGG 0.235891 4:-64394824 MsaT021278.1:CDS
AGAAGAACATGGATTATAAA+AGG 0.308077 4:+64395231 None:intergenic
CCGCTCTCATACTATTAATA+AGG 0.314029 4:-64395092 MsaT021278.1:CDS
AAATTCATAACAACTCCTTT+TGG 0.331715 4:+64394488 None:intergenic
CCTCCTGATAGAACGTTTGT+TGG 0.361348 4:+64394856 None:intergenic
TGCGCTGCGGCTGGTGGTGG+TGG 0.387987 4:-64394776 MsaT021278.1:CDS
AAATACTTGGTTAAGTCAAT+TGG 0.389715 4:+64394899 None:intergenic
AAACCAACAAACGTTCTATC+AGG 0.407703 4:-64394859 MsaT021278.1:CDS
GCTGGTGGTGGTGGTGATCA+TGG 0.417182 4:-64394767 MsaT021278.1:CDS
GGACTTTGGTGGCAAATACT+TGG 0.426874 4:+64394886 None:intergenic
AACTCACACTCATACATTCT+CGG 0.428608 4:+64395172 None:intergenic
ATGTCCGCGAAAAGCCTTGT+TGG 0.435044 4:+64395015 None:intergenic
TCTTGCGCTGCGGCTGGTGG+TGG 0.459861 4:-64394779 MsaT021278.1:CDS
TATGATATTGCACCATGAAA+AGG 0.464373 4:-64394993 MsaT021278.1:CDS
GGTTGACGATTGAATACTCT+TGG 0.485743 4:+64394967 None:intergenic
TCTAATGAATTGAAGCCAAA+AGG 0.489056 4:-64394503 MsaT021278.1:CDS
TCATGTGCAACATCTTCTGT+TGG 0.490922 4:+64394449 None:intergenic
TTGAAATTGTTCCAAGGATG+TGG 0.492696 4:+64394946 None:intergenic
AATATGTCTTGCGCTGCGGC+TGG 0.494836 4:-64394785 MsaT021278.1:CDS
TCAATCGTCAACCACATCCT+TGG 0.496081 4:-64394957 MsaT021278.1:CDS
GAACAATTTCAATTTCAAGT+TGG 0.498276 4:-64394935 MsaT021278.1:CDS
TTCAACTCTGGCAACGCACT+CGG 0.512404 4:-64395136 MsaT021278.1:CDS
GAAACAGAAACATAGTTGTC+CGG 0.516663 4:-64395059 MsaT021278.1:CDS
TTGAAATTGAAATTGTTCCA+AGG 0.519609 4:+64394940 None:intergenic
ATTATTATTGTCCTTCACCT+TGG 0.520250 4:+64394655 None:intergenic
ATGATATTGCACCATGAAAA+GGG 0.521004 4:-64394992 MsaT021278.1:CDS
TACTTGGTTAAGTCAATTGG+TGG 0.543017 4:+64394902 None:intergenic
ATGTCTTGCGCTGCGGCTGG+TGG 0.545212 4:-64394782 MsaT021278.1:CDS
TCTTTAATCTTGAATATCAG+CGG 0.572245 4:+64394605 None:intergenic
TGGTGACATTGATGAAACTT+CGG 0.577161 4:-64394681 MsaT021278.1:CDS
AAGGTCTCGCAATGCAGCCA+AGG 0.578454 4:-64394578 MsaT021278.1:CDS
TGATGAAACTTCGGCGACCA+AGG 0.579505 4:-64394672 MsaT021278.1:CDS
AGTGTGAGTTGTGCGGCAAA+AGG 0.579769 4:-64395159 MsaT021278.1:CDS
TAGTTGTCCGGTTTGCAACA+AGG 0.585771 4:-64395047 MsaT021278.1:CDS
TGCGGCAAAAGGTTCAACTC+TGG 0.586813 4:-64395148 MsaT021278.1:CDS
GACAAGGTCGAGAAACAAGT+TGG 0.597799 4:-64394701 MsaT021278.1:CDS
TTGGTTAAGTCAATTGGTGG+TGG 0.603616 4:+64394905 None:intergenic
AAGCAATGTGGACGATGACA+AGG 0.610743 4:-64394717 MsaT021278.1:CDS
AACTCTGGCAACGCACTCGG+TGG 0.616672 4:-64395133 MsaT021278.1:CDS
CTTCAATATGTCTTGCGCTG+CGG 0.618324 4:-64394789 MsaT021278.1:CDS
AACTTCGGCGACCAAGGTGA+AGG 0.625913 4:-64394666 MsaT021278.1:CDS
GGATTATAAAAGGAACAAGA+AGG 0.636811 4:+64395241 None:intergenic
AATGAATGAAAAGAAGAACA+TGG 0.641497 4:+64395220 None:intergenic
TGAAGGTGGTGAAACTGCGA+TGG 0.648342 4:-64394534 MsaT021278.1:CDS
CCAACAAACGTTCTATCAGG+AGG 0.665230 4:-64394856 MsaT021278.1:CDS
GCTCAAAGTTTCAAGCAATG+TGG 0.669423 4:-64394729 MsaT021278.1:CDS
ATTCAAGATTAAAGACAACA+AGG 0.676790 4:-64394597 MsaT021278.1:CDS
TATTAATAGTATGAGAGCGG+CGG 0.687350 4:+64395095 None:intergenic
CCTTATTAATAGTATGAGAG+CGG 0.698742 4:+64395092 None:intergenic
ATGTATGAGTGTGAGTTGTG+CGG 0.703631 4:-64395166 MsaT021278.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 64394445 64395281 64394445 ID=MsaG021278
Chr4 mRNA 64394445 64395281 64394445 ID=MsaT021278.1;Parent=MsaG021278
Chr4 exon 64394445 64395281 64394445 ID=MsaT021278.1.exon1;Parent=MsaT021278.1
Chr4 CDS 64394445 64395281 64394445 ID=cds.MsaT021278.1;Parent=MsaT021278.1
Gene Sequence

>MsaT021278.1

ATGAGGAAGAAACGTACTCCTTCTTGTTCCTTTTATAATCCATGTTCTTCTTTTCATTCATTCGAAGATAATGAGAAATCTCAAGCTCCGAGAATGTATGAGTGTGAGTTGTGCGGCAAAAGGTTCAACTCTGGCAACGCACTCGGTGGCCACAAAACATCTCACCGCCGCTCTCATACTATTAATAAGGACGATGATGAGAAACAGAAACATAGTTGTCCGGTTTGCAACAAGGTTTTTTCATCCAACAAGGCTTTTCGCGGACATATGATATTGCACCATGAAAAGGGTTCCAAGAGTATTCAATCGTCAACCACATCCTTGGAACAATTTCAATTTCAAGTTGGTATTGCACCACCACCAATTGACTTAACCAAGTATTTGCCACCAAAGTCCCATAAAACCAACAAACGTTCTATCAGGAGGAGAAATATCATTGATCATGAACATCTTAATGGAGCACAAACACTCTTCAATATGTCTTGCGCTGCGGCTGGTGGTGGTGGTGATCATGGTCACATACATAAAAAGCTCAAAGTTTCAAGCAATGTGGACGATGACAAGGTCGAGAAACAAGTTGGTGACATTGATGAAACTTCGGCGACCAAGGTGAAGGACAATAATAATAATAATAATAACAGTAAAAAGAGTTTGCCGCTGATATTCAAGATTAAAGACAACAAGGTCTCGCAATGCAGCCAAGGTGAAGAAAAGAAGAAAAATAATGAAGGTGGTGAAACTGCGATGGAAGAGAGTTCTAATGAATTGAAGCCAAAAGGAGTTGTTATGAATTTTGATTTGAATGAGCTTCCAACAGAAGATGTTGCACATGAATGA

Protein sequence

>MsaT021278.1

MRKKRTPSCSFYNPCSSFHSFEDNEKSQAPRMYECELCGKRFNSGNALGGHKTSHRRSHTINKDDDEKQKHSCPVCNKVFSSNKAFRGHMILHHEKGSKSIQSSTTSLEQFQFQVGIAPPPIDLTKYLPPKSHKTNKRSIRRRNIIDHEHLNGAQTLFNMSCAAAGGGGDHGHIHKKLKVSSNVDDDKVEKQVGDIDETSATKVKDNNNNNNNSKKSLPLIFKIKDNKVSQCSQGEEKKKNNEGGETAMEESSNELKPKGVVMNFDLNELPTEDVAHE*