Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG021278 | RHN79076.1 | 78.295 | 258 | 41 | 5 | 1 | 245 | 1 | 256 | 5.93e-131 | 382 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG021278 | tr|A0A396JLE5|A0A396JLE5_MEDTR | 78.295 | 258 | 41 | 5 | 1 | 245 | 1 | 256 | 2.83e-131 | 382 |
Gene ID | Type | Classification |
---|---|---|
MsaG021278 | TF | C2H2 |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG001179 | MsaG021278 | 0.808532 | 5.038366e-50 | 8.304854e-48 |
MsaG001414 | MsaG021278 | 0.889352 | 5.735192e-73 | 1.633989e-69 |
MsaG001415 | MsaG021278 | 0.854990 | 1.645965e-61 | 1.062936e-58 |
MsaG002009 | MsaG021278 | 0.801710 | 1.328134e-48 | 1.866475e-46 |
MsaG002016 | MsaG021278 | 0.819994 | 1.517924e-52 | 3.338053e-50 |
MsaG003288 | MsaG021278 | 0.808675 | 4.696927e-50 | 7.769090e-48 |
MsaG004319 | MsaG021278 | 0.849550 | 5.717741e-60 | 3.052075e-57 |
MsaG005148 | MsaG021278 | 0.810340 | 2.070451e-50 | 3.565241e-48 |
MsaG005149 | MsaG021278 | 0.842088 | 5.948354e-58 | 2.480616e-55 |
MsaG005150 | MsaG021278 | 0.834205 | 6.231470e-56 | 2.038244e-53 |
MsaG005205 | MsaG021278 | 0.903098 | 1.150188e-78 | 7.069188e-75 |
MsaG006533 | MsaG021278 | 0.825118 | 9.899785e-54 | 2.498399e-51 |
MsaG006601 | MsaG021278 | 0.813488 | 4.302182e-51 | 8.006394e-49 |
MsaG007428 | MsaG021278 | 0.811932 | 9.385947e-51 | 1.680487e-48 |
MsaG007429 | MsaG021278 | 0.801497 | 1.467971e-48 | 2.053030e-46 |
MsaG008047 | MsaG021278 | 0.875310 | 7.064001e-68 | 1.029779e-64 |
MsaG008208 | MsaG021278 | 0.888096 | 1.741955e-72 | 4.654750e-69 |
MsaG008229 | MsaG021278 | 0.822651 | 3.725541e-53 | 8.792392e-51 |
MsaG008426 | MsaG021278 | 0.855215 | 1.416710e-61 | 9.223393e-59 |
MsaG008550 | MsaG021278 | 0.869313 | 6.896904e-66 | 7.765349e-63 |
MsaG008956 | MsaG021278 | 0.819939 | 1.562305e-52 | 3.430548e-50 |
MsaG008961 | MsaG021278 | 0.856793 | 4.918668e-62 | 3.391804e-59 |
MsaG008967 | MsaG021278 | 0.850731 | 2.678316e-60 | 1.488591e-57 |
MsaG009100 | MsaG021278 | 0.828378 | 1.663017e-54 | 4.596538e-52 |
MsaG011115 | MsaG021278 | 0.812134 | 8.483647e-51 | 1.526398e-48 |
MsaG011296 | MsaG021278 | 0.800226 | 2.660459e-48 | 3.615455e-46 |
MsaG011505 | MsaG021278 | 0.813274 | 4.789770e-51 | 8.866163e-49 |
MsaG012273 | MsaG021278 | 0.836403 | 1.746282e-56 | 6.101286e-54 |
MsaG013646 | MsaG021278 | 0.804000 | 4.491321e-49 | 6.653138e-47 |
MsaG014099 | MsaG021278 | 0.816903 | 7.558870e-52 | 1.533764e-49 |
MsaG014541 | MsaG021278 | 0.870997 | 1.949543e-66 | 2.356770e-63 |
MsaG015152 | MsaG021278 | 0.804953 | 2.848655e-49 | 4.314083e-47 |
MsaG015178 | MsaG021278 | 0.808343 | 5.525719e-50 | 9.066732e-48 |
MsaG017328 | MsaG021278 | 0.849258 | 6.890817e-60 | 3.641405e-57 |
MsaG018183 | MsaG021278 | 0.832710 | 1.464779e-55 | 4.584968e-53 |
MsaG018341 | MsaG021278 | 0.808321 | 5.586154e-50 | 9.160881e-48 |
MsaG018343 | MsaG021278 | 0.810639 | 1.785700e-50 | 3.097343e-48 |
MsaG019140 | MsaG021278 | 0.815988 | 1.208573e-51 | 2.395505e-49 |
MsaG019451 | MsaG021278 | 0.816555 | 9.038662e-52 | 1.817645e-49 |
MsaG020314 | MsaG021278 | 0.820057 | 1.468533e-52 | 3.234836e-50 |
MsaG021076 | MsaG021278 | 0.833478 | 9.454219e-56 | 3.026748e-53 |
MsaG021278 | MsaG021279 | 0.931765 | 6.418540e-94 | 3.019119e-89 |
MsaG021278 | MsaG021280 | 0.852075 | 1.121318e-60 | 6.531230e-58 |
MsaG021278 | MsaG021281 | 0.914881 | 2.826816e-84 | 3.698535e-80 |
MsaG021278 | MsaG022010 | 0.843329 | 2.793966e-58 | 1.212496e-55 |
MsaG021278 | MsaG022011 | 0.822418 | 4.217739e-53 | 9.891840e-51 |
MsaG021278 | MsaG022058 | 0.834843 | 4.317334e-56 | 1.439396e-53 |
MsaG021278 | MsaG022059 | 0.802558 | 8.904092e-49 | 1.275834e-46 |
MsaG021278 | MsaG022683 | 0.813059 | 5.337830e-51 | 9.828030e-49 |
MsaG021278 | MsaG023089 | 0.841981 | 6.347369e-58 | 2.638102e-55 |
MsaG021278 | MsaG023408 | 0.834008 | 6.977941e-56 | 2.268953e-53 |
MsaG021278 | MsaG023516 | 0.802459 | 9.328857e-49 | 1.333664e-46 |
MsaG021278 | MsaG023917 | 0.890467 | 2.113776e-73 | 6.383831e-70 |
MsaG021278 | MsaG023835 | 0.871873 | 1.003262e-66 | 1.259175e-63 |
MsaG021278 | MsaG023837 | 0.866079 | 7.430485e-65 | 7.329924e-62 |
MsaG021278 | MsaG023838 | 0.865480 | 1.146576e-64 | 1.104202e-61 |
MsaG021278 | MsaG023842 | 0.821427 | 7.139399e-53 | 1.630572e-50 |
MsaG021278 | MsaG023856 | 0.835708 | 2.616491e-56 | 8.951815e-54 |
MsaG021278 | MsaG024025 | 0.826928 | 3.693412e-54 | 9.801391e-52 |
MsaG021278 | MsaG024026 | 0.836161 | 2.011164e-56 | 6.975456e-54 |
MsaG021278 | MsaG026990 | 0.810047 | 2.392312e-50 | 4.090052e-48 |
MsaG021278 | MsaG029184 | 0.861740 | 1.639090e-63 | 1.361824e-60 |
MsaG021278 | MsaG029340 | 0.839378 | 3.029341e-57 | 1.159649e-54 |
MsaG021278 | MsaG031277 | 0.824900 | 1.114005e-53 | 2.794685e-51 |
MsaG021278 | MsaG031436 | 0.807742 | 7.406599e-50 | 1.197878e-47 |
MsaG021278 | MsaG032998 | 0.814966 | 2.035516e-51 | 3.931541e-49 |
MsaG021278 | MsaG033354 | 0.819481 | 1.986376e-52 | 4.309255e-50 |
MsaG021278 | MsaG033437 | 0.808914 | 4.178327e-50 | 6.950971e-48 |
MsaG021278 | MsaG033812 | 0.800355 | 2.505728e-48 | 3.415160e-46 |
MsaG021278 | MsaG033814 | 0.856316 | 6.779533e-62 | 4.594638e-59 |
MsaG021278 | MsaG034658 | 0.825297 | 8.985418e-54 | 2.278778e-51 |
MsaG021278 | MsaG034659 | 0.826347 | 5.075877e-54 | 1.325391e-51 |
MsaG021278 | MsaG035373 | 0.855240 | 1.393604e-61 | 9.081418e-59 |
MsaG021278 | MsaG035375 | 0.830674 | 4.628369e-55 | 1.365496e-52 |
MsaG021278 | MsaG035376 | 0.851546 | 1.581785e-60 | 9.042219e-58 |
MsaG021278 | MsaG035378 | 0.856099 | 7.846565e-62 | 5.275357e-59 |
MsaG021278 | MsaG037863 | 0.862334 | 1.079803e-63 | 9.180706e-61 |
MsaG021278 | MsaG037865 | 0.815284 | 1.731758e-51 | 3.371796e-49 |
MsaG021278 | MsaG037867 | 0.819905 | 1.591147e-52 | 3.490620e-50 |
MsaG021278 | MsaG037869 | 0.832859 | 1.346018e-55 | 4.231402e-53 |
MsaG021278 | MsaG038395 | 0.853995 | 3.182638e-61 | 1.983796e-58 |
MsaG021278 | MsaG038533 | 0.867422 | 2.788752e-65 | 2.905021e-62 |
MsaG021278 | MsaG039671 | 0.818553 | 3.221748e-52 | 6.821072e-50 |
MsaG021278 | MsaG040071 | 0.833349 | 1.018055e-55 | 3.246807e-53 |
MsaG021278 | MsaG040072 | 0.836135 | 2.041633e-56 | 7.075652e-54 |
MsaG021278 | MsaG040077 | 0.803861 | 4.798488e-49 | 7.085206e-47 |
MsaG021278 | MsaG040775 | 0.817489 | 5.588442e-52 | 1.151208e-49 |
MsaG021278 | MsaG040820 | 0.832519 | 1.633374e-55 | 5.084156e-53 |
MsaG021278 | MsaG040823 | 0.827738 | 2.367994e-54 | 6.428225e-52 |
MsaG021278 | MsaG041049 | 0.841933 | 6.535148e-58 | 2.711979e-55 |
MsaG021278 | MsaG041561 | 0.841090 | 1.087089e-57 | 4.391884e-55 |
MsaG021278 | MsaG041687 | 0.830698 | 4.567186e-55 | 1.348353e-52 |
MsaG021278 | MsaG041888 | 0.827630 | 2.512259e-54 | 6.799278e-52 |
MsaG021278 | MsaG042406 | 0.842882 | 3.670510e-58 | 1.570185e-55 |
MsaG021278 | MsaG043369 | 0.820248 | 1.328543e-52 | 2.941437e-50 |
MsaG021278 | MsaG044352 | 0.815281 | 1.734245e-51 | 3.376380e-49 |
MsaG021278 | MsaG044380 | 0.802931 | 7.463783e-49 | 1.078619e-46 |
MsaG021278 | MsaG044794 | 0.851160 | 2.030403e-60 | 1.145332e-57 |
MsaG021278 | MsaG046120 | 0.823569 | 2.281579e-53 | 5.520020e-51 |
MsaG021278 | MsaG010012 | 0.801975 | 1.172392e-48 | 1.657583e-46 |
MsaG021278 | MsaG013262 | 0.854631 | 2.089042e-61 | 1.331910e-58 |
MsaG021278 | MsaG021538 | 0.857018 | 4.225704e-62 | 2.937755e-59 |
MsaG021278 | MsaG024399 | 0.866659 | 4.871651e-65 | 4.920841e-62 |
MsaG021278 | MsaG032859 | 0.846490 | 3.956606e-59 | 1.904627e-56 |
MsaG021278 | MsaG033509 | 0.842370 | 5.013248e-58 | 2.109711e-55 |
MsaG021278 | MsaG031902 | 0.912657 | 3.714412e-83 | 4.181020e-79 |
MsaG021278 | MsaG031903 | 0.868958 | 8.979004e-66 | 9.962042e-63 |
MsaG021278 | MsaG030523 | 0.821543 | 6.712432e-53 | 1.537835e-50 |
MsaG021278 | MsaG038716 | 0.805191 | 2.542221e-49 | 3.871371e-47 |
MsaG021278 | MsaG037163 | 0.834393 | 5.594902e-56 | 1.840399e-53 |
MsaG021278 | MsaG035298 | 0.828593 | 1.476411e-54 | 4.105310e-52 |
MsaG021278 | MsaG038131 | 0.828198 | 1.837352e-54 | 5.052504e-52 |
MsaG021278 | MsaG039770 | 0.808490 | 5.141063e-50 | 8.465731e-48 |
MsaG021278 | MsaG037023 | 0.859436 | 8.122760e-63 | 6.181433e-60 |
MsaG021278 | MsaG035340 | 0.805044 | 2.727304e-49 | 4.139114e-47 |
MsaG021278 | MsaG045440 | 0.830117 | 6.324492e-55 | 1.836506e-52 |
MsaG021278 | MsaG043831 | 0.808346 | 5.517555e-50 | 9.053957e-48 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG021278 | MtrunA17_Chr1g0172961 | 78.295 | 258 | 41 | 5 | 1 | 245 | 1 | 256 | 5.45e-135 | 382 |
MsaG021278 | MtrunA17_Chr4g0037041 | 87.050 | 139 | 16 | 1 | 1 | 137 | 1 | 139 | 1.11e-83 | 249 |
MsaG021278 | MtrunA17_Chr1g0172861 | 44.272 | 323 | 83 | 11 | 1 | 274 | 1 | 275 | 4.15e-57 | 184 |
MsaG021278 | MtrunA17_Chr1g0169521 | 34.967 | 306 | 133 | 11 | 20 | 278 | 28 | 314 | 2.03e-33 | 124 |
MsaG021278 | MtrunA17_Chr1g0169561 | 34.967 | 306 | 133 | 11 | 20 | 278 | 28 | 314 | 3.18e-33 | 123 |
MsaG021278 | MtrunA17_Chr7g0214511 | 39.200 | 250 | 104 | 12 | 42 | 278 | 71 | 285 | 6.73e-24 | 97.8 |
MsaG021278 | MtrunA17_Chr1g0174871 | 33.333 | 264 | 149 | 10 | 31 | 278 | 31 | 283 | 8.81e-24 | 97.8 |
MsaG021278 | MtrunA17_Chr1g0174861 | 35.047 | 214 | 112 | 7 | 23 | 235 | 25 | 212 | 2.92e-21 | 90.1 |
MsaG021278 | MtrunA17_Chr1g0174001 | 37.255 | 204 | 102 | 11 | 90 | 278 | 1 | 193 | 2.11e-14 | 70.1 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG021278 | AT2G45120.1 | 30.233 | 129 | 79 | 1 | 19 | 136 | 180 | 308 | 2.14e-11 | 63.5 |
Find 50 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
ATTGATCATGAACATCTTAA+TGG | 0.235891 | 4:-64394824 | MsaT021278.1:CDS |
AGAAGAACATGGATTATAAA+AGG | 0.308077 | 4:+64395231 | None:intergenic |
CCGCTCTCATACTATTAATA+AGG | 0.314029 | 4:-64395092 | MsaT021278.1:CDS |
AAATTCATAACAACTCCTTT+TGG | 0.331715 | 4:+64394488 | None:intergenic |
CCTCCTGATAGAACGTTTGT+TGG | 0.361348 | 4:+64394856 | None:intergenic |
TGCGCTGCGGCTGGTGGTGG+TGG | 0.387987 | 4:-64394776 | MsaT021278.1:CDS |
AAATACTTGGTTAAGTCAAT+TGG | 0.389715 | 4:+64394899 | None:intergenic |
AAACCAACAAACGTTCTATC+AGG | 0.407703 | 4:-64394859 | MsaT021278.1:CDS |
GCTGGTGGTGGTGGTGATCA+TGG | 0.417182 | 4:-64394767 | MsaT021278.1:CDS |
GGACTTTGGTGGCAAATACT+TGG | 0.426874 | 4:+64394886 | None:intergenic |
AACTCACACTCATACATTCT+CGG | 0.428608 | 4:+64395172 | None:intergenic |
ATGTCCGCGAAAAGCCTTGT+TGG | 0.435044 | 4:+64395015 | None:intergenic |
TCTTGCGCTGCGGCTGGTGG+TGG | 0.459861 | 4:-64394779 | MsaT021278.1:CDS |
TATGATATTGCACCATGAAA+AGG | 0.464373 | 4:-64394993 | MsaT021278.1:CDS |
GGTTGACGATTGAATACTCT+TGG | 0.485743 | 4:+64394967 | None:intergenic |
TCTAATGAATTGAAGCCAAA+AGG | 0.489056 | 4:-64394503 | MsaT021278.1:CDS |
TCATGTGCAACATCTTCTGT+TGG | 0.490922 | 4:+64394449 | None:intergenic |
TTGAAATTGTTCCAAGGATG+TGG | 0.492696 | 4:+64394946 | None:intergenic |
AATATGTCTTGCGCTGCGGC+TGG | 0.494836 | 4:-64394785 | MsaT021278.1:CDS |
TCAATCGTCAACCACATCCT+TGG | 0.496081 | 4:-64394957 | MsaT021278.1:CDS |
GAACAATTTCAATTTCAAGT+TGG | 0.498276 | 4:-64394935 | MsaT021278.1:CDS |
TTCAACTCTGGCAACGCACT+CGG | 0.512404 | 4:-64395136 | MsaT021278.1:CDS |
GAAACAGAAACATAGTTGTC+CGG | 0.516663 | 4:-64395059 | MsaT021278.1:CDS |
TTGAAATTGAAATTGTTCCA+AGG | 0.519609 | 4:+64394940 | None:intergenic |
ATTATTATTGTCCTTCACCT+TGG | 0.520250 | 4:+64394655 | None:intergenic |
ATGATATTGCACCATGAAAA+GGG | 0.521004 | 4:-64394992 | MsaT021278.1:CDS |
TACTTGGTTAAGTCAATTGG+TGG | 0.543017 | 4:+64394902 | None:intergenic |
ATGTCTTGCGCTGCGGCTGG+TGG | 0.545212 | 4:-64394782 | MsaT021278.1:CDS |
TCTTTAATCTTGAATATCAG+CGG | 0.572245 | 4:+64394605 | None:intergenic |
TGGTGACATTGATGAAACTT+CGG | 0.577161 | 4:-64394681 | MsaT021278.1:CDS |
AAGGTCTCGCAATGCAGCCA+AGG | 0.578454 | 4:-64394578 | MsaT021278.1:CDS |
TGATGAAACTTCGGCGACCA+AGG | 0.579505 | 4:-64394672 | MsaT021278.1:CDS |
AGTGTGAGTTGTGCGGCAAA+AGG | 0.579769 | 4:-64395159 | MsaT021278.1:CDS |
TAGTTGTCCGGTTTGCAACA+AGG | 0.585771 | 4:-64395047 | MsaT021278.1:CDS |
TGCGGCAAAAGGTTCAACTC+TGG | 0.586813 | 4:-64395148 | MsaT021278.1:CDS |
GACAAGGTCGAGAAACAAGT+TGG | 0.597799 | 4:-64394701 | MsaT021278.1:CDS |
TTGGTTAAGTCAATTGGTGG+TGG | 0.603616 | 4:+64394905 | None:intergenic |
AAGCAATGTGGACGATGACA+AGG | 0.610743 | 4:-64394717 | MsaT021278.1:CDS |
AACTCTGGCAACGCACTCGG+TGG | 0.616672 | 4:-64395133 | MsaT021278.1:CDS |
CTTCAATATGTCTTGCGCTG+CGG | 0.618324 | 4:-64394789 | MsaT021278.1:CDS |
AACTTCGGCGACCAAGGTGA+AGG | 0.625913 | 4:-64394666 | MsaT021278.1:CDS |
GGATTATAAAAGGAACAAGA+AGG | 0.636811 | 4:+64395241 | None:intergenic |
AATGAATGAAAAGAAGAACA+TGG | 0.641497 | 4:+64395220 | None:intergenic |
TGAAGGTGGTGAAACTGCGA+TGG | 0.648342 | 4:-64394534 | MsaT021278.1:CDS |
CCAACAAACGTTCTATCAGG+AGG | 0.665230 | 4:-64394856 | MsaT021278.1:CDS |
GCTCAAAGTTTCAAGCAATG+TGG | 0.669423 | 4:-64394729 | MsaT021278.1:CDS |
ATTCAAGATTAAAGACAACA+AGG | 0.676790 | 4:-64394597 | MsaT021278.1:CDS |
TATTAATAGTATGAGAGCGG+CGG | 0.687350 | 4:+64395095 | None:intergenic |
CCTTATTAATAGTATGAGAG+CGG | 0.698742 | 4:+64395092 | None:intergenic |
ATGTATGAGTGTGAGTTGTG+CGG | 0.703631 | 4:-64395166 | MsaT021278.1:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr4 | gene | 64394445 | 64395281 | 64394445 | ID=MsaG021278 |
Chr4 | mRNA | 64394445 | 64395281 | 64394445 | ID=MsaT021278.1;Parent=MsaG021278 |
Chr4 | exon | 64394445 | 64395281 | 64394445 | ID=MsaT021278.1.exon1;Parent=MsaT021278.1 |
Chr4 | CDS | 64394445 | 64395281 | 64394445 | ID=cds.MsaT021278.1;Parent=MsaT021278.1 |
Gene Sequence |
Protein sequence |