AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG021286


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021286 MtrunA17_Chr6g0452881 84.586 532 78 2 29 556 309 840 0.0 867
MsaG021286 MtrunA17_Chr6g0452891 73.469 245 37 3 285 529 1 217 4.76e-121 355
MsaG021286 MtrunA17_Chr4g0037071 88.265 196 23 0 347 542 1 196 7.07e-113 335
MsaG021286 MtrunA17_Chr4g0037081 88.950 181 18 1 1 181 1 179 5.55e-107 318
MsaG021286 MtrunA17_Chr7g0265781 34.682 173 109 3 362 532 848 1018 7.71e-33 134
MsaG021286 MtrunA17_Chr1g0179281 34.857 175 104 4 362 530 839 1009 1.21e-30 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG021286 AT4G04885.1 45.234 535 233 15 46 556 310 808 4.69e-125 386
MsaG021286 AT1G66500.1 34.349 361 170 15 210 535 66 394 6.46e-46 167
MsaG021286 AT5G43620.1 35.667 300 120 11 279 535 119 388 2.74e-44 162
MsaG021286 AT2G36480.2 32.164 171 107 3 362 532 786 947 3.36e-24 107
MsaG021286 AT2G36480.1 32.164 171 107 3 362 532 786 947 3.36e-24 107

Find 144 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATAATGCACATGTATTT+TGG 0.206485 4:+64565048 None:intergenic
AAACAGCTCTTTCGGTTTCC+TGG 0.212509 4:-64565684 MsaT021286.1:CDS
TGCGCGAGGGCTTCTGCTTA+TGG 0.240620 4:+64565207 None:intergenic
ATGCGGAGCATGCACATTTA+TGG 0.262216 4:+64566081 None:intergenic
TTATTCCATTAGGCGTATTA+AGG 0.289551 4:+64564961 None:intergenic
AAGAGCTGTTTGCAATTCTT+TGG 0.289612 4:+64565696 None:intergenic
TGACGAACCTTCAGAAGATT+TGG 0.298615 4:+64565362 None:intergenic
ATGCTTGTTGTTATTCCATT+AGG 0.303075 4:+64564951 None:intergenic
GCTTACTGCCAGAAGTTTCT+TGG 0.305569 4:+64566389 None:intergenic
TCTTTGGCTGCCTTCTTCAT+AGG 0.310252 4:+64564339 None:intergenic
TTGCCACCTTTGCCTCCAGC+TGG 0.323915 4:-64565653 MsaT021286.1:CDS
AGAAACCATTACTTCTACTA+TGG 0.330076 4:+64566251 None:intergenic
ATAACTGATGAAGAATTCTT+TGG 0.332236 4:+64566520 None:intergenic
ATGCATTTAGCATGAATAAT+GGG 0.333257 4:+64564909 None:intergenic
GATTTGAATGGATTCCTAAC+AGG 0.333799 4:+64566022 None:intergenic
TCTAGGAAGATCGCCATATA+AGG 0.342211 4:+64565322 None:intergenic
TCTGCAATCAGTGCCTTATA+TGG 0.351640 4:-64565335 MsaT021286.1:CDS
GGTTTCTTGCAACCAACTAT+TGG 0.360158 4:-64566235 MsaT021286.1:CDS
TATGCATTTAGCATGAATAA+TGG 0.367648 4:+64564908 None:intergenic
GGTAGAGCATGTGGGCCAGC+TGG 0.367975 4:+64565638 None:intergenic
AACAGCTCTTTCGGTTTCCT+GGG 0.369608 4:-64565683 MsaT021286.1:CDS
TCAGCGTCAAGGGTGTTTCC+TGG 0.373151 4:-64566166 MsaT021286.1:CDS
TGGCAGTAAGCCTTTGTTAG+TGG 0.376982 4:-64566377 MsaT021286.1:CDS
TCAAAAGTTGCTGGCAGAAC+TGG 0.381258 4:+64566115 None:intergenic
TTAGCATTTATTGATTTGAA+TGG 0.385462 4:+64566010 None:intergenic
TAGGCATCAATTAGAGCTCT+TGG 0.388106 4:+64566427 None:intergenic
AGAATTGCAAACAGCTCTTT+CGG 0.388388 4:-64565692 MsaT021286.1:CDS
GGCATAACAAGTTGTGCATT+TGG 0.389349 4:+64565734 None:intergenic
GCTGATTAGGCAACTGATAC+AGG 0.398235 4:+64565900 None:intergenic
TAACAAGTTGTGCATTTGGC+AGG 0.415660 4:+64565738 None:intergenic
GACAAAAGCCAAGAAACTTC+TGG 0.416293 4:-64566397 MsaT021286.1:CDS
GATTGGGATTAGGTAGAATC+TGG 0.424134 4:+64565615 None:intergenic
ATGACTTCTGTGCTCATCTT+GGG 0.424211 4:+64565268 None:intergenic
AAAGAAAGAAGATGAAGAGT+TGG 0.425274 4:-64565090 MsaT021286.1:CDS
TATGACTTCTGTGCTCATCT+TGG 0.426913 4:+64565267 None:intergenic
GGATTCTTCGATGGAAATAT+AGG 0.431337 4:+64566043 None:intergenic
CTGTTTGCAATTCTTTGGCC+TGG 0.431824 4:+64565701 None:intergenic
CTTAGTGGTGCAGAGGCATT+GGG 0.433928 4:-64565158 MsaT021286.1:CDS
GAAGGACCAGGATTGGGATT+AGG 0.436097 4:+64565605 None:intergenic
GTGGACAAACGCCACCATTC+CGG 0.438771 4:-64565844 MsaT021286.1:CDS
TTCATCTTGGTGGGAATACT+CGG 0.444524 4:-64566552 MsaT021286.1:CDS
GACACAGAAGGACCAGGATT+GGG 0.445177 4:+64565599 None:intergenic
TTGGGAGCTGAAGCAGCTCC+AGG 0.449579 4:-64565140 MsaT021286.1:CDS
GCTTAGTGGTGCAGAGGCAT+TGG 0.449928 4:-64565159 MsaT021286.1:CDS
GCGTTTGTCCACTGTTATGC+GGG 0.451893 4:+64565855 None:intergenic
GTATACCTTAATACGCCTAA+TGG 0.453857 4:-64564966 MsaT021286.1:CDS
AGATGAGCACAGAAGTCATA+TGG 0.461058 4:-64565264 MsaT021286.1:CDS
CGCCTCGTTTCAAAAGTTGC+TGG 0.461501 4:+64566106 None:intergenic
TGGAAATATAGGAGTTATGC+TGG 0.461860 4:+64566054 None:intergenic
TAAGTAAATTGTGACGCTGC+TGG 0.462016 4:+64565803 None:intergenic
TTCCATCGAAGAATCCTGTT+AGG 0.463431 4:-64566036 MsaT021286.1:CDS
GCTCATCTTGGGACTTGAAT+CGG 0.465641 4:+64565279 None:intergenic
CTTTGGCCTGGCGAAGGAAG+TGG 0.465990 4:+64565713 None:intergenic
TCACTGTAAAACTTGTCAAA+AGG 0.474303 4:+64565020 None:intergenic
GCCTTTGTTAGTGGAGCGAC+CGG 0.474907 4:-64566368 MsaT021286.1:CDS
GGCCCAAGGTGTTATGTCAT+TGG 0.480316 4:-64565528 MsaT021286.1:CDS
CCATGTTCATGCACAGGAAA+AGG 0.480750 4:+64565953 None:intergenic
TATTGGTTTCTCATCAGAAA+AGG 0.481430 4:-64566218 MsaT021286.1:CDS
TCTGATGAGAAACCAATAGT+TGG 0.483041 4:+64566223 None:intergenic
ACTTATAGCACTTCTTTGCC+AGG 0.484285 4:-64565785 MsaT021286.1:CDS
TGACACAGAAGGACCAGGAT+TGG 0.501011 4:+64565598 None:intergenic
ATCAGACCGGGTAGCTGATT+AGG 0.502180 4:+64565887 None:intergenic
CTGAAGAGGAGGAGTTCGAT+TGG 0.502985 4:-64566300 MsaT021286.1:CDS
CCACAAGTTGTGCACTGTCT+AGG 0.505428 4:+64565305 None:intergenic
TCATCAAAACATCTCTTTCG+CGG 0.506613 4:+64566487 None:intergenic
TAATTTGATTAGTTCACTCA+TGG 0.508846 4:-64565549 MsaT021286.1:CDS
AGAGCTCTAATTGATGCCTA+TGG 0.514260 4:-64566424 MsaT021286.1:CDS
TGTGGGCCAGCTGGAGGCAA+AGG 0.514981 4:+64565647 None:intergenic
AGCCAATGACATAACACCTT+GGG 0.518658 4:+64565526 None:intergenic
ACAGTGATGAAACGGAGGAT+TGG 0.518817 4:-64565004 MsaT021286.1:CDS
ACGGAGGATTGGATGTATAG+AGG 0.522404 4:-64564993 MsaT021286.1:CDS
ATAGATTTAGTTACCAGCGC+AGG 0.524466 4:+64565494 None:intergenic
AGCATGGACAGGTCGCAGTT+AGG 0.525989 4:-64564933 MsaT021286.1:CDS
CCATCACAAAATATTCAGAC+GGG 0.528028 4:+64564874 None:intergenic
GGAGTTATGCTGGGAGGATG+CGG 0.529614 4:+64566064 None:intergenic
GGCTAATCAAGCTCCTGCGC+TGG 0.529795 4:-64565507 MsaT021286.1:intron
AGCACAGAAGTCATATGGAT+TGG 0.534514 4:-64565259 MsaT021286.1:CDS
TGAAGAGGAGGAGTTCGATT+GGG 0.536389 4:-64566299 MsaT021286.1:CDS
CTACTATGGTCTACTAGTGT+TGG 0.539108 4:+64566265 None:intergenic
CAGCTCTTTCGGTTTCCTGG+GGG 0.541573 4:-64565681 MsaT021286.1:CDS
CTCATAGCAGCACAATGTCC+TGG 0.542263 4:+64565767 None:intergenic
AACAAGTTGTGCATTTGGCA+GGG 0.544007 4:+64565739 None:intergenic
AATATCCAACAGTTGGCTGT+TGG 0.544187 4:+64565573 None:intergenic
ACAGCTCTTTCGGTTTCCTG+GGG 0.544294 4:-64565682 MsaT021286.1:CDS
AAATTAGAATATCCAACAGT+TGG 0.545058 4:+64565566 None:intergenic
TGCAACTACTTCAGCGTCAA+GGG 0.545212 4:-64566176 MsaT021286.1:CDS
AGCAGAAGCCCTCGCGCAAG+TGG 0.548139 4:-64565202 MsaT021286.1:CDS
GGGCCAGCTGGAGGCAAAGG+TGG 0.549350 4:+64565650 None:intergenic
GCAATTCTTTGGCCTGGCGA+AGG 0.555122 4:+64565707 None:intergenic
GTTATGCCACTTCCTTCGCC+AGG 0.555543 4:-64565719 MsaT021286.1:CDS
ACAAGTTGTGCATTTGGCAG+GGG 0.557390 4:+64565740 None:intergenic
GGCGTTTGTCCACTGTTATG+CGG 0.558202 4:+64565854 None:intergenic
GTGCAACTACTTCAGCGTCA+AGG 0.559076 4:-64566177 MsaT021286.1:CDS
AGAATCTGGGGTAGAGCATG+TGG 0.560420 4:+64565629 None:intergenic
AAAGTTGCTGGCAGAACTGG+TGG 0.562453 4:+64566118 None:intergenic
TTCCTAACAGGATTCTTCGA+TGG 0.563320 4:+64566034 None:intergenic
CAAGTTGTGCATTTGGCAGG+GGG 0.563383 4:+64565741 None:intergenic
AGTAGTTGCACTTGCTGCAA+CGG 0.563425 4:+64566189 None:intergenic
ATCAAGAAGATGCTTGCTGA+TGG 0.563854 4:-64566577 None:intergenic
ATTCTACCTAATCCCAATCC+TGG 0.565745 4:-64565611 MsaT021286.1:CDS
AGAGCATGTGGGCCAGCTGG+AGG 0.566008 4:+64565641 None:intergenic
TGGCTTGACACAGAAGGACC+AGG 0.566253 4:+64565593 None:intergenic
GGCTGTTGGCTTGACACAGA+AGG 0.567803 4:+64565587 None:intergenic
TCCATCACAAAATATTCAGA+CGG 0.568109 4:+64564873 None:intergenic
TCAAGCCAACAGCCAACTGT+TGG 0.568482 4:-64565578 MsaT021286.1:CDS
GACGCTGCTGGATCCTGAGA+TGG 0.569203 4:+64565815 None:intergenic
CGGGTTAGATGAAATCAGAC+CGG 0.570016 4:+64565874 None:intergenic
GTAGACCATAGTAGAAGTAA+TGG 0.573238 4:-64566256 MsaT021286.1:CDS
ATTAGTTCACTCATGGCCCA+AGG 0.574805 4:-64565542 MsaT021286.1:CDS
GTTTGATCCAAATCTTCTGA+AGG 0.578645 4:-64565369 MsaT021286.1:CDS
TCCGGTCGCTCCACTAACAA+AGG 0.582967 4:+64566367 None:intergenic
ATGCAGGGCACCTATGAAGA+AGG 0.583079 4:-64564349 MsaT021286.1:intron
CCTAGACAGTGCACAACTTG+TGG 0.586792 4:-64565305 MsaT021286.1:CDS
TCAACACCATGTTCATGCAC+AGG 0.588454 4:+64565947 None:intergenic
GAATAACAACAAGCATGGAC+AGG 0.588860 4:-64564944 MsaT021286.1:CDS
TAATGGAATAACAACAAGCA+TGG 0.589626 4:-64564949 MsaT021286.1:CDS
GTTGGCAGTTCTTGCTGACG+AGG 0.590256 4:-64565072 MsaT021286.1:CDS
AGCTCTTTCGGTTTCCTGGG+GGG 0.591033 4:-64565680 MsaT021286.1:CDS
ACTCACAAACCACTTGCGCG+AGG 0.597010 4:+64565193 None:intergenic
AGTGAGACGATGTGGCTTAG+TGG 0.601641 4:-64565173 MsaT021286.1:CDS
CTCACAAACCACTTGCGCGA+GGG 0.611864 4:+64565194 None:intergenic
GGAAATATAGGAGTTATGCT+GGG 0.611878 4:+64566055 None:intergenic
GGGTTAGATGAAATCAGACC+GGG 0.617202 4:+64565875 None:intergenic
ATTGGGATTAGGTAGAATCT+GGG 0.617573 4:+64565616 None:intergenic
AATAGTACCAGTGTGAGTCA+TGG 0.620953 4:-64565989 MsaT021286.1:CDS
TAGCCAATGACATAACACCT+TGG 0.621234 4:+64565525 None:intergenic
TGCATTTAGCATGAATAATG+GGG 0.625810 4:+64564910 None:intergenic
ATGGCAGAATACTGAAGAGG+AGG 0.632607 4:-64566311 MsaT021286.1:CDS
CAGTTGCCTAATCAGCTACC+CGG 0.639744 4:-64565893 MsaT021286.1:CDS
AATTAGACCATGACTCACAC+TGG 0.642372 4:+64565982 None:intergenic
ATAAGTTTATGACAACATCA+TGG 0.644139 4:-64566330 MsaT021286.1:CDS
CATCTTGGGACTTGAATCGG+AGG 0.650361 4:+64565282 None:intergenic
TCAATATTAGAATTGAGACC+AGG 0.653967 4:+64566148 None:intergenic
GATGTGGCTTAGTGGTGCAG+AGG 0.663497 4:-64565165 MsaT021286.1:CDS
ATCATGGCAGAATACTGAAG+AGG 0.665757 4:-64566314 MsaT021286.1:CDS
AATATAGGAGTTATGCTGGG+AGG 0.677336 4:+64566058 None:intergenic
TCATCTAACCCGCATAACAG+TGG 0.680332 4:-64565863 MsaT021286.1:CDS
GAATCTGGGGTAGAGCATGT+GGG 0.680646 4:+64565630 None:intergenic
CATCACAAAATATTCAGACG+GGG 0.686925 4:+64564875 None:intergenic
TTGGGATTAGGTAGAATCTG+GGG 0.686989 4:+64565617 None:intergenic
GCAGCCAAAGAAAACGAATG+CGG 0.688645 4:-64564327 MsaT021286.1:CDS
TTGCTGGCAGAACTGGTGGA+CGG 0.691595 4:+64566122 None:intergenic
GGTTTGTGAGTGAGACGATG+TGG 0.711325 4:-64565181 MsaT021286.1:CDS
TTTGCCAACCGAGACCACTG+AGG 0.774441 4:-64565114 MsaT021286.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 64564321 64566590 64564321 ID=MsaG021286
Chr4 mRNA 64564321 64566590 64564321 ID=MsaT021286.1;Parent=MsaG021286
Chr4 exon 64564321 64564365 64564321 ID=MsaT021286.1.exon3;Parent=MsaT021286.1
Chr4 CDS 64564321 64564365 64564321 ID=cds.MsaT021286.1;Parent=MsaT021286.1
Chr4 exon 64564866 64565408 64564866 ID=MsaT021286.1.exon2;Parent=MsaT021286.1
Chr4 CDS 64564866 64565408 64564866 ID=cds.MsaT021286.1;Parent=MsaT021286.1
Chr4 exon 64565508 64566590 64565508 ID=MsaT021286.1.exon1;Parent=MsaT021286.1
Chr4 CDS 64565508 64566590 64565508 ID=cds.MsaT021286.1;Parent=MsaT021286.1
Gene Sequence

>MsaT021286.1

ATGCTTGCTGATGGTTTTCATCTTGGTGGGAATACTCGGAGCGAAATACCAAAGAATTCTTCATCAGTTATCTCAAACATTCCGCGAAAGAGATGTTTTGATGAGTTAAGCTATCTCAACAACGAACAACCGTGTCAAAATCCAAGAGCTCTAATTGATGCCTATGGTAGTGACAAAAGCCAAGAAACTTCTGGCAGTAAGCCTTTGTTAGTGGAGCGACCGGATAAAAACGGTATGGATAAGTTTATGACAACATCATGGCAGAATACTGAAGAGGAGGAGTTCGATTGGGAAGATATGAGTCCAACACTAGTAGACCATAGTAGAAGTAATGGTTTCTTGCAACCAACTATTGGTTTCTCATCAGAAAAGGCTGTTACCGTTGCAGCAAGTGCAACTACTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATATTGAGTACCGTCCACCAGTTCTGCCAGCAACTTTTGAAACGAGGCGCTCCATAAATGTGCATGCTCCGCATCCTCCCAGCATAACTCCTATATTTCCATCGAAGAATCCTGTTAGGAATCCATTCAAATCAATAAATGCTAATAGTACCAGTGTGAGTCATGGTCTAATTAACAGACCTTTTCCTGTGCATGAACATGGTGTTGAGAACAACGATACCAGTAAAAGAAACCTGTATCAGTTGCCTAATCAGCTACCCGGTCTGATTTCATCTAACCCGCATAACAGTGGACAAACGCCACCATTCCGGTTTTTTCCATCTCAGGATCCAGCAGCGTCACAATTTACTTATAGCACTTCTTTGCCAGGACATTGTGCTGCTATGAGTAACCCCCTGCCAAATGCACAACTTGTTATGCCACTTCCTTCGCCAGGCCAAAGAATTGCAAACAGCTCTTTCGGTTTCCTGGGGGGAGCTTTGCCACCTTTGCCTCCAGCTGGCCCACATGCTCTACCCCAGATTCTACCTAATCCCAATCCTGGTCCTTCTGTGTCAAGCCAACAGCCAACTGTTGGATATTCTAATTTGATTAGTTCACTCATGGCCCAAGGTGTTATGTCATTGGCTAATCAAGCTCCTGCGCTGGATTTTGTTGGCATTGAGTTTGATCCAAATCTTCTGAAGGTTCGTCATGAATCTGCAATCAGTGCCTTATATGGCGATCTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAGTCCCAAGATGAGCACAGAAGTCATATGGATTGGCATGTGACTAAGAACCGTATGTCTAAAAACCATAAGCAGAAGCCCTCGCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTTAGTGGTGCAGAGGCATTGGGAGCTGAAGCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAGGAAAAGAAAGAAGATGAAGAGTTGGCAGTTCTTGCTGACGAGGACCAAAATACATGTGCATTATGTAGAGAACCTTTTGACAAGTTTTACAGTGATGAAACGGAGGATTGGATGTATAGAGGAGCTGTATACCTTAATACGCCTAATGGAATAACAACAAGCATGGACAGGTCGCAGTTAGGCCCCATTATTCATGCTAAATGCATATCTGAATCTACCCCGTCTGAATATTTTGTGATGGATGAAGGGGGCACCTATGAAGAAGGCAGCCAAAGAAAACGAATGCGGAGCTGA

Protein sequence

>MsaT021286.1

MLADGFHLGGNTRSEIPKNSSSVISNIPRKRCFDELSYLNNEQPCQNPRALIDAYGSDKSQETSGSKPLLVERPDKNGMDKFMTTSWQNTEEEEFDWEDMSPTLVDHSRSNGFLQPTIGFSSEKAVTVAASATTSASRVFPGLNSNIEYRPPVLPATFETRRSINVHAPHPPSITPIFPSKNPVRNPFKSINANSTSVSHGLINRPFPVHEHGVENNDTSKRNLYQLPNQLPGLISSNPHNSGQTPPFRFFPSQDPAASQFTYSTSLPGHCAAMSNPLPNAQLVMPLPSPGQRIANSSFGFLGGALPPLPPAGPHALPQILPNPNPGPSVSSQQPTVGYSNLISSLMAQGVMSLANQAPALDFVGIEFDPNLLKVRHESAISALYGDLPRQCTTCGLRFKSQDEHRSHMDWHVTKNRMSKNHKQKPSRKWFVSETMWLSGAEALGAEAAPGFLPTETTEEKKEDEELAVLADEDQNTCALCREPFDKFYSDETEDWMYRGAVYLNTPNGITTSMDRSQLGPIIHAKCISESTPSEYFVMDEGGTYEEGSQRKRMRS*