AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG022203

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG022203 MtrunA17_Chr1g0180581 49.778 225 52 8 2 199 1 191 4.27e-54 172
MsaG022203 MtrunA17_Chr1g0193731 53.333 225 58 11 8 203 15 221 1.52e-53 172
MsaG022203 MtrunA17_Chr7g0214511 41.901 284 65 10 3 203 19 285 1.38e-46 154
MsaG022203 MtrunA17_Chr1g0169561 36.864 236 71 11 31 203 94 314 5.09e-29 110
MsaG022203 MtrunA17_Chr1g0169521 36.437 247 77 12 23 203 82 314 1.90e-28 108
MsaG022203 MtrunA17_Chr1g0174001 34.896 192 63 4 72 203 4 193 2.65e-24 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAGCGCCTGCCGTTTCT+TGG 0.192635 4:+75237655 None:intergenic
CTGAGCGCCTGCCGTTTCTT+GGG 0.253410 4:+75237656 None:intergenic
GAAAGAGAAAGAAGGGTATT+TGG 0.272932 4:+75237910 None:intergenic
TGAATCACAGCGGCACTAAA+AGG 0.331082 4:-75237523 MsaT022203.1:CDS
GGGTATTTGGCATTGGTTGT+TGG 0.420713 4:+75237923 None:intergenic
AAGCATTGAATGAACATAAG+AGG 0.452841 4:-75237751 MsaT022203.1:CDS
TGAGCGCCTGCCGTTTCTTG+GGG 0.456531 4:+75237657 None:intergenic
CTGGAAAACAAAAGATTACA+TGG 0.458402 4:-75237871 MsaT022203.1:CDS
GAAAGAAGGGTATTTGGCAT+TGG 0.461253 4:+75237916 None:intergenic
TCATTCAAATCCATATTGCT+TGG 0.468097 4:+75237491 None:intergenic
TATCAATGAAAGAGAAAGAA+GGG 0.475697 4:+75237903 None:intergenic
TTCATTGATACTAATCATGC+TGG 0.476827 4:-75237890 MsaT022203.1:CDS
GCTGTGATTCACATCGATAT+TGG 0.491587 4:+75237535 None:intergenic
GAGCGCCTGCCGTTTCTTGG+GGG 0.509966 4:+75237658 None:intergenic
CTCATGTGATGTTTGCAACA+AGG 0.514548 4:-75237789 MsaT022203.1:CDS
ACTCATAATCACAATGATGT+TGG 0.520742 4:-75237827 MsaT022203.1:CDS
AGCGCCTGCCGTTTCTTGGG+GGG 0.523006 4:+75237659 None:intergenic
GTATCAATGAAAGAGAAAGA+AGG 0.525178 4:+75237902 None:intergenic
GGGTAGAAACTGATCATCAA+TGG 0.529257 4:+75237679 None:intergenic
TCATCTTCAAAATCATCACT+TGG 0.541142 4:+75237365 None:intergenic
ATATCTCGCGAGGGTTATGA+AGG 0.547888 4:-75237566 MsaT022203.1:CDS
AACTGATCATCAATGGAAGA+TGG 0.551680 4:+75237686 None:intergenic
AAGAGGTGTCACTCTCAAAA+CGG 0.554314 4:-75237734 MsaT022203.1:CDS
TTAACTTCAAAATCATCACT+TGG 0.557014 4:+75237464 None:intergenic
TATGTTGCATATATCTCGCG+AGG 0.599224 4:-75237576 MsaT022203.1:CDS
ATGGAAGATGGTGCCGCAGT+TGG 0.604901 4:+75237698 None:intergenic
ACATCATTGTGATTATGAGT+AGG 0.629484 4:+75237830 None:intergenic
TATTACTAACACACCAACTG+CGG 0.645228 4:-75237711 MsaT022203.1:CDS
ATGTTGCATATATCTCGCGA+GGG 0.700679 4:-75237575 MsaT022203.1:CDS
AATATCGATGTGAATCACAG+CGG 0.797032 4:-75237533 MsaT022203.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 75237364 75237972 75237364 ID=MsaG022203
Chr4 mRNA 75237364 75237972 75237364 ID=MsaT022203.1;Parent=MsaG022203
Chr4 exon 75237364 75237972 75237364 ID=MsaT022203.1.exon1;Parent=MsaT022203.1
Chr4 CDS 75237364 75237972 75237364 ID=cds.MsaT022203.1;Parent=MsaT022203.1
Gene Sequence

>MsaT022203.1

TTCATGAACAAGAAAATGTCTCATAAACCAACAACCAATGCCAAATACCCTTCTTTCTCTTTCATTGATACTAATCATGCTGGAAAACAAAAGATTACATGGTTTTTTTCTTATTCTGTTCCTACTCATAATCACAATGATGTTGGAAGACAAAACAAACACTCATGTGATGTTTGCAACAAGGTTTTTACTTCTAACAAAGCATTGAATGAACATAAGAGGTGTCACTCTCAAAACGGTATTACTAACACACCAACTGCGGCACCATCTTCCATTGATGATCAGTTTCTACCCCCCAAGAAACGGCAGGCGCTCAGAAGAAAAAGATATATGGATATCGATGATGATGATGATGAAGTTACTGCTGCTGAAAATATGTTGCATATATCTCGCGAGGGTTATGAAGGATCAACTGCCAATATCGATGTGAATCACAGCGGCACTAAAAGGCAAAAAAATCCAAGCAATATGGATTTGAATGAGCTTCCAAGTGATGATTTTGAAGTTAATGAGGAGAAGAAGATTAACGAAGCTGAAACTGAAGTCGACTCAAAAGTTGTGAAGAATTTTGATTTGAACGAGCTTCCAAGTGATGATTTTGAAGATGAA
Protein sequence

>MsaT022203.1

FMNKKMSHKPTTNAKYPSFSFIDTNHAGKQKITWFFSYSVPTHNHNDVGRQNKHSCDVCNKVFTSNKALNEHKRCHSQNGITNTPTAAPSSIDDQFLPPKKRQALRRKRYMDIDDDDDEVTAAENMLHISREGYEGSTANIDVNHSGTKRQKNPSNMDLNELPSDDFEVNEEKKINEAETEVDSKVVKNFDLNELPSDDFEDE