AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023524


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023524 MtrunA17_Chr4g0037381 64.964 137 3 1 1 92 1 137 2.96e-53 162
MsaG023524 MtrunA17_Chr8g0371801 58.333 72 30 0 1 72 1 72 7.43e-26 97.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023524 AT2G21060.1 61.644 73 28 0 3 75 12 84 4.85e-28 101
MsaG023524 AT2G17870.1 64.789 71 25 0 2 72 7 77 1.42e-27 102
MsaG023524 AT4G36020.2 60.563 71 28 0 2 72 7 77 3.55e-25 95.1
MsaG023524 AT4G36020.1 59.722 72 29 0 1 72 6 77 6.61e-25 95.1
MsaG023524 AT4G36020.3 59.722 72 29 0 1 72 6 77 6.61e-25 95.1
MsaG023524 AT4G38680.1 60.274 73 29 0 3 75 8 80 2.07e-22 86.7

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGTGGGTCAAAATTAAC+TGG 0.306323 4:+89603800 MsaT023524.1:CDS
TTCAATGATCAGAAGGGGTT+TGG 0.339445 4:+89603836 MsaT023524.1:CDS
AACTCCTCACTTCCATCATC+AGG 0.391515 4:-89603869 None:intergenic
TGCTACAGCTGTGGTGAATC+TGG 0.396062 4:+89604343 MsaT023524.1:intron
TTTGCTAGAGATTGTCCAAC+AGG 0.434784 4:+89604370 MsaT023524.1:CDS
AATTAACTGGGAAGGTGAAA+TGG 0.440654 4:+89603813 MsaT023524.1:CDS
TCCAAGGCTGTTAGTGTGAC+TGG 0.440806 4:+89603989 MsaT023524.1:CDS
GGATTCATCACCCCTGATGA+TGG 0.483119 4:+89603857 MsaT023524.1:CDS
CTTCAACGAGCGCTGCCTGT+TGG 0.500201 4:-89604385 None:intergenic
CATCAGTCTGGATCTGAGAT+TGG 0.500424 4:-89603901 None:intergenic
TCCAGTCACACTAACAGCCT+TGG 0.511590 4:-89603990 None:intergenic
GAGAACGGAATCCATCAGTC+TGG 0.513134 4:-89603913 None:intergenic
AAAATTCAATTCATCATGAG+TGG 0.553878 4:+89603785 None:intergenic
GTTAGTGTGACTGGACCTGA+TGG 0.557938 4:+89603998 MsaT023524.1:CDS
CAATCTCAGATCCAGACTGA+TGG 0.559889 4:+89603902 MsaT023524.1:CDS
GGATTCCGTTCTCTTGCTGA+AGG 0.562087 4:+89603923 MsaT023524.1:CDS
GAAATGGTTCAATGATCAGA+AGG 0.565831 4:+89603829 MsaT023524.1:CDS
AGATCTGAGTAAAGTGGTGG+TGG 0.576919 4:+89604406 MsaT023524.1:CDS
CGTTGAAGATCTGAGTAAAG+TGG 0.585840 4:+89604400 MsaT023524.1:CDS
ACTCCTCACTTCCATCATCA+GGG 0.589005 4:-89603868 None:intergenic
ATTCACCTTCAGCAAGAGAA+CGG 0.616654 4:-89603928 None:intergenic
TGATAACGATGGAAGATCCA+AGG 0.617668 4:+89603973 MsaT023524.1:CDS
AAATGGTTCAATGATCAGAA+GGG 0.619071 4:+89603830 MsaT023524.1:CDS
CACCCCTGATGATGGAAGTG+AGG 0.619233 4:+89603865 MsaT023524.1:CDS
AATGGTTCAATGATCAGAAG+GGG 0.619776 4:+89603831 MsaT023524.1:CDS
TGGGTCAAAATTAACTGGGA+AGG 0.622767 4:+89603805 MsaT023524.1:CDS
AAATTCAATTCATCATGAGT+GGG 0.626143 4:+89603786 None:intergenic
CAGATTGAGTCTGATAACGA+TGG 0.643460 4:+89603962 MsaT023524.1:CDS
CTCCTCACTTCCATCATCAG+GGG 0.654781 4:-89603867 None:intergenic
TGAGTGGGTCAAAATTAACT+GGG 0.660344 4:+89603801 MsaT023524.1:CDS
TGAAGATCTGAGTAAAGTGG+TGG 0.733020 4:+89604403 MsaT023524.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 89603800 89604433 89603800 ID=MsaG023524
Chr4 mRNA 89603800 89604433 89603800 ID=MsaT023524.1;Parent=MsaG023524
Chr4 exon 89603800 89604019 89603800 ID=MsaT023524.1.exon1;Parent=MsaT023524.1
Chr4 CDS 89603800 89604019 89603800 ID=cds.MsaT023524.1;Parent=MsaT023524.1
Chr4 exon 89604351 89604433 89604351 ID=MsaT023524.1.exon2;Parent=MsaT023524.1
Chr4 CDS 89604351 89604433 89604351 ID=cds.MsaT023524.1;Parent=MsaT023524.1
Gene Sequence

>MsaT023524.1

ATGAGTGGGTCAAAATTAACTGGGAAGGTGAAATGGTTCAATGATCAGAAGGGGTTTGGATTCATCACCCCTGATGATGGAAGTGAGGAGTTGTTTGTTCACCAATCTCAGATCCAGACTGATGGATTCCGTTCTCTTGCTGAAGGTGAATCTGTTGAGTATCAGATTGAGTCTGATAACGATGGAAGATCCAAGGCTGTTAGTGTGACTGGACCTGATGCTGTGGTGAATCTGGTCATTTTGCTAGAGATTGTCCAACAGGCAGCGCTCGTTGAAGATCTGAGTAAAGTGGTGGTGGTTTAG

Protein sequence

>MsaT023524.1

MSGSKLTGKVKWFNDQKGFGFITPDDGSEELFVHQSQIQTDGFRSLAEGESVEYQIESDNDGRSKAVSVTGPDAVVNLVILLEIVQQAALVEDLSKVVVV*