Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG023697 | AET04474.1 | 88.462 | 156 | 18 | 0 | 1 | 156 | 1 | 156 | 3.25e-91 | 272 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG023697 | tr|G7L9K0|G7L9K0_MEDTR | 88.462 | 156 | 18 | 0 | 1 | 156 | 1 | 156 | 1.55e-91 | 272 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG023697 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATACCTTCAAATGAAAT+TGG | 0.206576 | 4:+91535099 | MsaT023697.1:CDS |
| CATAAAACTGGCTTGTCTTT+TGG | 0.254968 | 4:-91534086 | None:intergenic |
| CTTTGAGGTTTCCCCAGTTT+TGG | 0.256311 | 4:-91534130 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.314815 | 4:-91534059 | None:intergenic |
| ATCCTCAAACAACGTCTCTT+TGG | 0.317203 | 4:-91534952 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4:+91534245 | MsaT023697.1:CDS |
| CTGTTAGTAACAAGTTCTTT+TGG | 0.373720 | 4:-91534176 | None:intergenic |
| TTTGGTCCCTGGCGCCATGC+TGG | 0.374123 | 4:-91534068 | None:intergenic |
| CTCCAAAGAGACGTTGTTTG+AGG | 0.374170 | 4:+91534950 | MsaT023697.1:CDS |
| GAAAACAACGTTTGAGAGAA+TGG | 0.387976 | 4:-91534154 | None:intergenic |
| CCTTCAAATGAAATTGGCCT+TGG | 0.396727 | 4:+91535105 | MsaT023697.1:CDS |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4:+91534117 | MsaT023697.1:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4:-91534098 | None:intergenic |
| CCAAGGCCAATTTCATTTGA+AGG | 0.421742 | 4:-91535105 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.436034 | 4:+91534061 | MsaT023697.1:CDS |
| TCCCTTCTACATAGAAGAAC+CGG | 0.461538 | 4:-91535148 | None:intergenic |
| GTTTGAGAGAATGGTCTTTG+AGG | 0.475222 | 4:-91534145 | None:intergenic |
| TCTTCTGAGGATGACTCATC+TGG | 0.485391 | 4:-91535048 | None:intergenic |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4:-91534240 | None:intergenic |
| GCAGTTCTTCTTAAACCAGC+CGG | 0.561335 | 4:+91535129 | MsaT023697.1:CDS |
| GAACAATAAGGCCTCTTCTG+AGG | 0.569819 | 4:-91535061 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.585142 | 4:+91534062 | MsaT023697.1:CDS |
| AGATGAGTCATCCTCAGAAG+AGG | 0.600054 | 4:+91535050 | MsaT023697.1:CDS |
| AAAGAACTTGTTACTAACAG+TGG | 0.608058 | 4:+91534179 | MsaT023697.1:CDS |
| TTCTACATAGAAGAACCGGC+TGG | 0.612139 | 4:-91535144 | None:intergenic |
| CAATCAGAAGATGACACCGA+TGG | 0.614691 | 4:+91534978 | MsaT023697.1:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4:+91534118 | MsaT023697.1:CDS |
| GTTTAAGAAGAACTGCTCCA+AGG | 0.642127 | 4:-91535122 | None:intergenic |
| CATCACCAGCAACTCCAGCA+TGG | 0.651498 | 4:+91534054 | MsaT023697.1:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4:+91534119 | MsaT023697.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 91534047 | 91535164 | 91534047 | ID=MsaG023697 |
| Chr4 | mRNA | 91534047 | 91535164 | 91534047 | ID=MsaT023697.1;Parent=MsaG023697 |
| Chr4 | exon | 91534047 | 91534266 | 91534047 | ID=MsaT023697.1.exon1;Parent=MsaT023697.1 |
| Chr4 | CDS | 91534047 | 91534266 | 91534047 | ID=cds.MsaT023697.1;Parent=MsaT023697.1 |
| Chr4 | exon | 91534914 | 91535164 | 91534914 | ID=MsaT023697.1.exon2;Parent=MsaT023697.1 |
| Chr4 | CDS | 91534914 | 91535164 | 91534914 | ID=cds.MsaT023697.1;Parent=MsaT023697.1 |
| Gene Sequence |
| Protein sequence |
