AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023698


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATACCTTCAAATGAAAT+TGG 0.214941 4:+91542613 MsaT023698.1:CDS
CTTTGAGGTTTCCCCAGTTT+TGG 0.256311 4:-91542163 None:intergenic
CATAAAACTGGCTTGTCTTC+TGG 0.266801 4:-91542119 None:intergenic
ATTCTCAAACTACGTCTCTT+TGG 0.299791 4:-91542466 None:intergenic
TCAAACAATAAGGCCTCTTC+TGG 0.301830 4:-91542577 None:intergenic
TGGCGCCATGCTGGAGTTGC+TGG 0.314815 4:-91542092 None:intergenic
GATGTTTCTGATTCAGATAA+TGG 0.318368 4:+91542278 MsaT023698.1:CDS
CTGTTCGTAACAAGTGCTTT+TGG 0.342724 4:-91542209 None:intergenic
TCTGGTCCCTGGCGCCATGC+TGG 0.378150 4:-91542101 None:intergenic
GAAAACAACGTTTGAGAGAA+TGG 0.387976 4:-91542187 None:intergenic
GTGGACTAAGATACCAAAAC+TGG 0.405891 4:+91542150 MsaT023698.1:CDS
CCACATGCATTGCATAAAAC+TGG 0.410323 4:-91542131 None:intergenic
CCTTCAAATGAAATTGGCCT+TGG 0.419265 4:+91542619 MsaT023698.1:CDS
CCAAGGCCAATTTCATTTGA+AGG 0.421742 4:-91542619 None:intergenic
AGCAACTCCAGCATGGCGCC+AGG 0.436034 4:+91542094 MsaT023698.1:CDS
GTTTGAGAGAATGGTCTTTG+AGG 0.475222 4:-91542178 None:intergenic
TCTTCTGGAGATGACTCATC+TGG 0.489997 4:-91542562 None:intergenic
CTGGCTTGTCTTCTGGTCCC+TGG 0.494515 4:-91542112 None:intergenic
TCTTGTTGAAACTTCTCCAT+CGG 0.530728 4:-91542508 None:intergenic
ATCTGAATCAGAAACATCAA+TGG 0.539843 4:-91542273 None:intergenic
TTCTACATAGAAGAACCGTC+TGG 0.563851 4:-91542658 None:intergenic
GCAACTCCAGCATGGCGCCA+GGG 0.585142 4:+91542095 MsaT023698.1:CDS
AGATGAGTCATCTCCAGAAG+AGG 0.604525 4:+91542564 MsaT023698.1:CDS
GCGGTTCTTCTTAAACCAGA+CGG 0.605404 4:+91542643 MsaT023698.1:CDS
AAATGAAATTGGCCTTGGAG+CGG 0.608265 4:+91542624 MsaT023698.1:CDS
CAATCAGAAGATGACACCGA+TGG 0.614119 4:+91542492 MsaT023698.1:CDS
TGGACTAAGATACCAAAACT+GGG 0.618651 4:+91542151 MsaT023698.1:CDS
GTTTAAGAAGAACCGCTCCA+AGG 0.637380 4:-91542636 None:intergenic
AAAGCACTTGTTACGAACAG+TGG 0.651202 4:+91542212 MsaT023698.1:CDS
CATCACCAGCAACTCCAGCA+TGG 0.651498 4:+91542087 MsaT023698.1:CDS
GGACTAAGATACCAAAACTG+GGG 0.731002 4:+91542152 MsaT023698.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 91542080 91542678 91542080 ID=MsaG023698
Chr4 mRNA 91542080 91542678 91542080 ID=MsaT023698.1;Parent=MsaG023698
Chr4 exon 91542080 91542299 91542080 ID=MsaT023698.1.exon1;Parent=MsaT023698.1
Chr4 CDS 91542080 91542299 91542080 ID=cds.MsaT023698.1;Parent=MsaT023698.1
Chr4 exon 91542428 91542678 91542428 ID=MsaT023698.1.exon2;Parent=MsaT023698.1
Chr4 CDS 91542428 91542678 91542428 ID=cds.MsaT023698.1;Parent=MsaT023698.1
Gene Sequence

>MsaT023698.1

ATGGATTCATCACCAGCAACTCCAGCATGGCGCCAGGGACCAGAAGACAAGCCAGTTTTATGCAATGCATGTGGACTAAGATACCAAAACTGGGGAAACCTCAAAGACCATTCTCTCAAACGTTGTTTTCCAAAAGCACTTGTTACGAACAGTGGCAACAGTAGCAACAACAGTCTCAAAGAAAGTTCATCAACCATTGATGTTTCTGATTCAGATAATGATGAAAAAGATTCAGATTCATGCAAGTCTGCAGTCTCTCCAAAGAGACGTAGTTTGAGAATTATCAATCAGAAGATGACACCGATGGAGAAGTTTCAACAAGAACTTCTCGATGAGTTGAAAAATCACGAGATACCAGATGAGTCATCTCCAGAAGAGGCCTTATTGTTTGAAAATGTGAACAACTTCATACCTTCAAATGAAATTGGCCTTGGAGCGGTTCTTCTTAAACCAGACGGTTCTTCTATGTAG

Protein sequence

>MsaT023698.1

MDSSPATPAWRQGPEDKPVLCNACGLRYQNWGNLKDHSLKRCFPKALVTNSGNSSNNSLKESSSTIDVSDSDNDEKDSDSCKSAVSPKRRSLRIINQKMTPMEKFQQELLDELKNHEIPDESSPEEALLFENVNNFIPSNEIGLGAVLLKPDGSSM*