AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023707

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023707 MtrunA17_Chr8g0380981 96.027 1032 34 1 15 1039 25 1056 0.0 2061
MsaG023707 MtrunA17_Chr2g0303101 60.635 1039 347 14 2 1036 21 1001 0.0 1164
MsaG023707 MtrunA17_Chr1g0189211 57.092 564 235 2 14 574 131 690 0.0 671
MsaG023707 MtrunA17_Chr1g0189211 30.380 316 180 5 732 1036 941 1227 8.87e-40 160
MsaG023707 MtrunA17_Chr7g0261401 43.304 575 310 7 15 575 99 671 4.76e-150 468
MsaG023707 MtrunA17_Chr5g0397501 38.964 444 225 6 136 568 288 696 1.39e-85 302
MsaG023707 MtrunA17_Chr5g0397501 50.000 74 33 1 28 101 23 92 3.41e-14 77.8
MsaG023707 MtrunA17_Chr5g0410721 37.376 404 172 11 30 421 86 420 6.08e-61 224
MsaG023707 MtrunA17_Chr1g0164731 36.693 387 172 9 29 407 41 362 4.29e-56 205
MsaG023707 MtrunA17_Chr3g0116441 34.988 403 191 10 33 425 18 359 8.51e-53 202
MsaG023707 MtrunA17_Chr1g0164761 34.278 388 162 9 29 407 4 307 4.20e-52 192
MsaG023707 MtrunA17_Chr1g0199511 39.367 221 124 3 229 439 272 492 4.99e-41 164
MsaG023707 MtrunA17_Chr7g0214951 42.609 115 41 4 275 381 104 201 1.38e-17 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023707 AT1G08620.2 44.244 999 480 15 14 936 127 1124 0.0 776
MsaG023707 AT1G08620.3 44.244 999 480 15 14 936 141 1138 0.0 776
MsaG023707 AT1G08620.4 44.244 999 480 15 14 936 127 1124 0.0 776
MsaG023707 AT1G08620.1 44.244 999 480 15 14 936 127 1124 0.0 776
MsaG023707 AT4G20400.1 59.612 567 224 3 2 566 25 588 0.0 717
MsaG023707 AT4G20400.1 41.772 237 106 3 803 1035 743 951 6.40e-46 179
MsaG023707 AT1G30810.3 62.238 572 206 5 2 570 28 592 0.0 705
MsaG023707 AT1G30810.3 50.267 187 80 3 788 974 646 819 6.31e-49 188
MsaG023707 AT1G30810.2 62.238 572 206 5 2 570 28 592 0.0 705
MsaG023707 AT1G30810.2 50.267 187 80 3 788 974 646 819 6.31e-49 188
MsaG023707 AT1G30810.1 62.238 572 206 5 2 570 28 592 0.0 705
MsaG023707 AT1G30810.1 50.267 187 80 3 788 974 646 819 6.31e-49 188
MsaG023707 AT2G34880.1 59.649 570 203 10 5 566 33 583 0.0 686
MsaG023707 AT2G34880.1 46.734 199 95 3 775 973 618 805 3.58e-43 170
MsaG023707 AT4G20400.2 60.227 528 205 3 41 566 7 531 0.0 669
MsaG023707 AT4G20400.2 41.772 237 106 3 803 1035 686 894 7.68e-46 179
MsaG023707 AT2G38950.1 42.235 528 277 4 29 556 104 603 6.09e-136 427
MsaG023707 AT1G63490.1 40.898 423 206 6 142 554 38 426 2.06e-90 315
MsaG023707 AT1G63490.2 40.898 423 206 6 142 554 291 679 1.69e-89 315
MsaG023707 AT1G63490.2 54.412 68 27 1 34 101 32 95 1.36e-13 76.3
MsaG023707 AT1G63490.4 40.898 423 206 6 142 554 291 679 1.80e-89 315
MsaG023707 AT1G63490.4 54.412 68 27 1 34 101 32 95 1.37e-13 76.3
MsaG023707 AT5G46910.2 34.592 503 239 16 32 523 102 525 1.86e-62 225
MsaG023707 AT5G46910.1 34.394 503 240 15 32 523 102 525 5.48e-62 226
MsaG023707 AT5G04240.1 31.746 441 237 11 33 422 16 443 2.11e-46 182
MsaG023707 AT3G48430.1 31.884 414 209 11 33 422 20 384 1.62e-41 166
MsaG023707 AT1G63490.3 38.655 238 123 4 322 554 1 220 8.04e-41 164

Find 231 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCATGATAAGTTGTTATT+TGG 0.119436 4:-91604754 MsaT023707.1:CDS
GTCATTCCTGCTGGATTTCT+TGG 0.180288 4:-91603221 MsaT023707.1:CDS
AAAATCTAAAATGTTTAAAT+TGG 0.189270 4:-91604678 MsaT023707.1:CDS
TCTAGAAAGATTCCTGAAAA+AGG 0.206821 4:-91602495 MsaT023707.1:CDS
ATCACTACCTTTACCATCTT+TGG 0.219822 4:+91606308 None:intergenic
TATCGAACTGTCCAGCATTC+TGG 0.224376 4:-91604931 MsaT023707.1:CDS
CTGCAAATGCACTCAGATTT+AGG 0.254376 4:-91603587 MsaT023707.1:CDS
CTTAAAGAATTTCAGCAATA+TGG 0.256142 4:-91606369 MsaT023707.1:CDS
ACACTTATTGTGACGGATTT+CGG 0.268838 4:+91603775 None:intergenic
AGTTCTTTGTATTTGAATAT+TGG 0.274068 4:-91603839 MsaT023707.1:CDS
CCTTTCGCCATCCATTATTC+AGG 0.275215 4:-91602870 MsaT023707.1:intron
TCTGAATCTGATGATAAGTT+TGG 0.276549 4:-91606417 MsaT023707.1:CDS
TTCGGCTGAGCTTGTTAGTT+TGG 0.276868 4:-91603465 MsaT023707.1:CDS
GGTACACCATACCATACTTT+TGG 0.284346 4:+91605352 None:intergenic
CAAGTTAATTCTGGATCTTC+AGG 0.284422 4:-91602668 MsaT023707.1:CDS
ATCAATGGTCATAAAATGTT+TGG 0.302237 4:-91602896 MsaT023707.1:CDS
CATATCTCTTTCTCCTTAAG+AGG 0.303958 4:+91606606 None:intergenic
CATCAATCCACAACACAAAA+AGG 0.310969 4:-91607079 MsaT023707.1:intron
CGAGAGACGGGAACCGTCTT+TGG 0.313567 4:-91606274 MsaT023707.1:CDS
AATCACAAACAAGTTAATTC+TGG 0.322127 4:-91602677 MsaT023707.1:CDS
ACCAACTCGTTGAGTAGATT+TGG 0.332672 4:+91605262 None:intergenic
AATAACTTTGCACGACTTCC+AGG 0.333657 4:-91605996 MsaT023707.1:CDS
TGTGGATCTGACTGGTGATA+TGG 0.338493 4:-91603918 MsaT023707.1:CDS
CCAACTAATGTTCGAAATTC+CGG 0.341000 4:-91603644 MsaT023707.1:CDS
ACAGGTGTTTCACCGATTAT+AGG 0.342856 4:+91606841 None:intergenic
AATGGATAAAAGATTTGTTC+TGG 0.348982 4:-91604248 MsaT023707.1:CDS
TCGGCTGAGCTTGTTAGTTT+GGG 0.349268 4:-91603464 MsaT023707.1:CDS
AGTTTGCATTACCTGAATAA+TGG 0.351329 4:+91602859 None:intergenic
CGAAATTCCGGTACACTTGA+TGG 0.353346 4:-91603632 MsaT023707.1:CDS
CCGGAATTTCGAACATTAGT+TGG 0.354395 4:+91603644 None:intergenic
CAAAGATGGGAACGTGTAAT+AGG 0.357690 4:-91606476 MsaT023707.1:CDS
GATAAGTTTGGATTCCAATC+AGG 0.359070 4:-91606405 MsaT023707.1:CDS
GGCTGCTCTTCAAACAAATC+AGG 0.359934 4:+91605283 None:intergenic
GGCTCCACCTTGCCCTCTTA+AGG 0.362816 4:-91606619 MsaT023707.1:CDS
TGCTGGTGGTATTTCTGTCT+TGG 0.365569 4:-91603687 MsaT023707.1:CDS
TGTCAAAGATATACTTGTAT+TGG 0.365838 4:+91603520 None:intergenic
TATGGAGCTGACTTGGAAAC+TGG 0.379640 4:-91606101 MsaT023707.1:CDS
CAATAAAGTTAAGGAGGATA+AGG 0.382232 4:-91603864 MsaT023707.1:CDS
ATCGAACTGTCCAGCATTCT+GGG 0.385690 4:-91604930 MsaT023707.1:CDS
CTTTCAGAGACAACTCTTCA+TGG 0.388357 4:-91604706 MsaT023707.1:CDS
ATCTGAATCACTCTCGGTTA+AGG 0.389849 4:+91606046 None:intergenic
TTCAGCATCTTTAAATGATC+CGG 0.400591 4:+91604412 None:intergenic
GCAAATCAACTTGCTGAATA+CGG 0.404788 4:+91606562 None:intergenic
ACTGGGGTGACCCAAAAGTA+TGG 0.407723 4:-91605363 MsaT023707.1:CDS
TCCTCAGACTTGAGTATTGA+AGG 0.408574 4:+91604964 None:intergenic
CTCTTAAGGAGAAAGAGATA+TGG 0.410495 4:-91606605 MsaT023707.1:CDS
ATTCACTTAATTATTTGCAC+TGG 0.411918 4:-91605381 MsaT023707.1:CDS
TGTTCTTACCAATGCAGTTA+AGG 0.414403 4:-91604632 MsaT023707.1:intron
ATCCAAATAACAACTTATCA+TGG 0.414820 4:+91604752 None:intergenic
CTTGATGGTTGTAAATTGTT+TGG 0.418077 4:-91603617 MsaT023707.1:CDS
ACTGGGATCTTGAGCTTCAA+TGG 0.419115 4:+91602721 None:intergenic
AAATATATTCCAGGTTACTT+TGG 0.419381 4:-91603059 MsaT023707.1:intron
ACATTGGTTGAAGCATTAGA+AGG 0.420604 4:-91604199 MsaT023707.1:CDS
GTGTTTGTGAAAACCTCGTT+GGG 0.424363 4:+91603028 None:intergenic
GAAGCATGTCGGCCTATAAT+CGG 0.425042 4:-91606853 MsaT023707.1:CDS
CTTCGATTCGGTTGAGGAAA+GGG 0.427057 4:-91604377 MsaT023707.1:CDS
TCAGATTTGTATGCTATGTC+TGG 0.427067 4:-91606029 MsaT023707.1:CDS
AGAGAGGCTTGAATGGCTTC+CGG 0.431090 4:-91604431 MsaT023707.1:CDS
ACATAGTTACAGATGCTTGA+TGG 0.431354 4:+91603251 None:intergenic
ATATTTAGCAAAGAATTTGC+AGG 0.436457 4:+91603800 None:intergenic
GTTTGTGAAGTCATTCCTGC+TGG 0.436870 4:-91603230 MsaT023707.1:CDS
TGCTTCGACTGGATCCCATC+TGG 0.438506 4:+91606870 None:intergenic
CTTAATAGTATGCTTGAAAC+GGG 0.448898 4:-91603557 MsaT023707.1:CDS
GACTTGGAAACTGGAGCACT+TGG 0.449548 4:-91606092 MsaT023707.1:CDS
AGTTTGGGATCTGTTGTGTA+TGG 0.451056 4:-91603449 MsaT023707.1:CDS
TTGCATGCACGAACAAGATA+TGG 0.452082 4:-91604107 MsaT023707.1:CDS
GGTCATGGACAATAAAGTTA+AGG 0.453833 4:-91603873 MsaT023707.1:CDS
GGAACGTGTAATAGGAGAAC+TGG 0.458459 4:-91606468 MsaT023707.1:CDS
AATAAAGTTAAGGAGGATAA+GGG 0.464006 4:-91603863 MsaT023707.1:CDS
GACTTGCACCTCTCTGCTGT+TGG 0.469090 4:-91604334 MsaT023707.1:CDS
AATCATTACCTTAACTGCAT+TGG 0.469539 4:+91604624 None:intergenic
GTCATGAATGTGTAGGGTAC+TGG 0.470928 4:-91602700 MsaT023707.1:CDS
TCGGTTAAGGATGATGTCTT+AGG 0.471679 4:+91606059 None:intergenic
TGGTGTCGATTTGATAAAGC+TGG 0.472194 4:-91602570 MsaT023707.1:CDS
AAAAGATCGCCTTCTTGTAC+AGG 0.472566 4:-91604046 MsaT023707.1:CDS
CGCATTGCTCGTAACTCGTC+AGG 0.474777 4:+91602467 None:intergenic
GAAACTGGAGCACTTGGAAG+TGG 0.475135 4:-91606086 MsaT023707.1:CDS
AGGATATTGAAGGTGAATAC+TGG 0.475212 4:-91606251 MsaT023707.1:CDS
CCATGCATAAACCAATCATA+TGG 0.479121 4:+91604826 None:intergenic
TCTTAAGGAGAAAGAGATAT+GGG 0.479506 4:-91606604 MsaT023707.1:CDS
CAAAGATATACTTGTATTGG+AGG 0.479875 4:+91603523 None:intergenic
AAATTGTCCCTCCAGCATGC+TGG 0.482121 4:-91606641 MsaT023707.1:CDS
ACTTAATAGTATGCTTGAAA+CGG 0.483422 4:-91603558 MsaT023707.1:CDS
TTTGAAGGAAGTGACATCTC+AGG 0.484096 4:-91605960 MsaT023707.1:CDS
CAAGGTGGAGCCCAGCATGC+TGG 0.485176 4:+91606630 None:intergenic
CAATGACTTCGATTCGGTTG+AGG 0.485568 4:-91604383 MsaT023707.1:CDS
ACACCTCAGCAGATAAGTGC+TGG 0.487268 4:-91603009 MsaT023707.1:CDS
ACGTGGAAAGCAGTCAAAGA+TGG 0.489358 4:-91606490 MsaT023707.1:CDS
TGAATGTGGCTCCATATGAT+TGG 0.492536 4:-91604837 MsaT023707.1:CDS
AATTCTTTAAGTGTGAAGTC+TGG 0.492628 4:+91606381 None:intergenic
TTAAGAGGTACCTCAAACGT+TGG 0.493058 4:+91606811 None:intergenic
ATAGCTGAAGCATGGCTGCC+AGG 0.493388 4:+91605331 None:intergenic
TTCACTTAATTATTTGCACT+GGG 0.494248 4:-91605380 MsaT023707.1:CDS
CTAAGACAAGAATAGTTGTC+AGG 0.496486 4:+91604298 None:intergenic
AGCCGATGAGGAAGAAAAGT+AGG 0.498687 4:-91606527 MsaT023707.1:CDS
CAGTCACATTTCTTCAGAGT+TGG 0.502183 4:-91603981 MsaT023707.1:CDS
TTGACAGACAAGGATAATGA+TGG 0.505126 4:-91604458 MsaT023707.1:intron
AATTTGTTATCACGTTTCCA+AGG 0.505646 4:-91604906 MsaT023707.1:CDS
TAACAAATTCCCCAGAATGC+TGG 0.506467 4:+91604920 None:intergenic
TGAACTAGACATGCCTCCCT+TGG 0.507459 4:-91602933 MsaT023707.1:CDS
CGTCCCTTAGCTGAACCATA+TGG 0.507902 4:-91606672 MsaT023707.1:CDS
GCAAATAATTAAGTGAATAG+AGG 0.508670 4:+91605386 None:intergenic
AATTGTCCCTCCAGCATGCT+GGG 0.509145 4:-91606640 MsaT023707.1:CDS
CAAATCAACTTGCTGAATAC+GGG 0.511163 4:+91606563 None:intergenic
CCGTCTTTGGAGGATATTGA+AGG 0.513289 4:-91606261 MsaT023707.1:CDS
TTAGTTCCATGGCTGTATGT+TGG 0.515006 4:-91605933 MsaT023707.1:CDS
TTGAAGGAAGTGACATCTCA+GGG 0.515084 4:-91605959 MsaT023707.1:CDS
AACTTCTCGTACACCTTATG+TGG 0.516226 4:-91603936 MsaT023707.1:CDS
GAGACGGGAACCGTCTTTGG+AGG 0.516381 4:-91606271 MsaT023707.1:CDS
GTTGTTATTTGGATCTGCTA+AGG 0.517525 4:-91604743 MsaT023707.1:CDS
ATAAATAAGCGCAAGAGTCT+CGG 0.517683 4:-91602956 MsaT023707.1:CDS
GATGGACAATGACTTCGATT+CGG 0.519329 4:-91604389 MsaT023707.1:CDS
AAGCCACAGTGGTATGCCCT+TGG 0.519658 4:+91604889 None:intergenic
TCATTTAAAGATGCTGAAGA+TGG 0.519800 4:-91604407 MsaT023707.1:CDS
AAACTGAGAATTACAAAGAA+GGG 0.521718 4:-91604069 MsaT023707.1:CDS
GTGTGAAGTCTGGCCCTGAT+TGG 0.521936 4:+91606391 None:intergenic
ATTTGTATGCTATGTCTGGC+TGG 0.521950 4:-91606025 MsaT023707.1:CDS
ATAATAATCACCACGAGAGA+CGG 0.528045 4:-91606287 MsaT023707.1:CDS
AAGCATGCAATATATCCAAA+AGG 0.529982 4:-91603410 MsaT023707.1:intron
CTAAGGGACGTATCTTAGCT+AGG 0.531048 4:+91606685 None:intergenic
ACTTCGATTCGGTTGAGGAA+AGG 0.531289 4:-91604378 MsaT023707.1:CDS
CGTGGAAAGCAGTCAAAGAT+GGG 0.533229 4:-91606489 MsaT023707.1:CDS
GAGCTACTGCACAACATCAA+TGG 0.533625 4:-91602911 MsaT023707.1:CDS
ATAGGCCGACATGCTTCGAC+TGG 0.534634 4:+91606859 None:intergenic
TCTATCAGCACTTTCAGCGC+AGG 0.535328 4:+91602404 None:intergenic
TAAATTGGAGAAATGCTTGT+GGG 0.535780 4:-91604663 MsaT023707.1:CDS
GGTTCAAGTAATAGCTCTCT+AGG 0.535894 4:-91602614 MsaT023707.1:CDS
ATGCCATATGGTTCAGCTAA+GGG 0.537203 4:+91606669 None:intergenic
GATGACTCTAAGTTAACTTG+TGG 0.541352 4:-91602635 MsaT023707.1:CDS
TACCACTGTGGCTTCAGCAG+TGG 0.544420 4:-91604880 MsaT023707.1:CDS
GGTGTATTATGGAGCTGACT+TGG 0.544686 4:-91606108 MsaT023707.1:CDS
TCAAAACATAATGCAGAACC+TGG 0.546610 4:+91605978 None:intergenic
TTTCATGATGAGACTCTGAA+TGG 0.546643 4:+91603957 None:intergenic
AGTTAACTTAGAGTCATCAA+TGG 0.547322 4:+91602640 None:intergenic
TCTTTCTCCTTAAGAGGGCA+AGG 0.549473 4:+91606612 None:intergenic
AAAACTGAGAATTACAAAGA+AGG 0.549929 4:-91604070 MsaT023707.1:CDS
AAAGTATGGTATGGTGTACC+TGG 0.550723 4:-91605349 MsaT023707.1:CDS
GTACCCTACACATTCATGAC+TGG 0.551735 4:+91602703 None:intergenic
TCCAAATAACAACTTATCAT+GGG 0.552438 4:+91604753 None:intergenic
ATAAGTTTGGATTCCAATCA+GGG 0.554076 4:-91606404 MsaT023707.1:CDS
TAGGAGTTTGAAGATACGCT+TGG 0.554441 4:-91606711 MsaT023707.1:CDS
ATGTGACTGTGAGGACTGCT+AGG 0.556875 4:+91603995 None:intergenic
TCACCAGTCAGATCCACATA+AGG 0.560398 4:+91603923 None:intergenic
CCATATGATTGGTTTATGCA+TGG 0.561009 4:-91604826 MsaT023707.1:CDS
ATCCCAGTCATGAATGTGTA+GGG 0.562524 4:-91602706 MsaT023707.1:CDS
ACATTAGTTGGACATGGTGA+AGG 0.562740 4:+91603656 None:intergenic
TGCCGCAGATATCATCCAGA+TGG 0.562975 4:-91606885 MsaT023707.1:intron
ATGGAACACACTTATTGTGA+CGG 0.563827 4:+91603768 None:intergenic
AGTGATGCCAAAGATGGTAA+AGG 0.565685 4:-91606315 MsaT023707.1:CDS
CATGAAGAGTTGTCTCTGAA+AGG 0.565752 4:+91604707 None:intergenic
TGCATTACCTGAATAATGGA+TGG 0.567093 4:+91602863 None:intergenic
TAATAATCACCACGAGAGAC+GGG 0.572482 4:-91606286 MsaT023707.1:CDS
ATATCTCTTTCTCCTTAAGA+GGG 0.572645 4:+91606607 None:intergenic
AGTTGTTTCTGCTAGTGTTG+AGG 0.574329 4:-91604134 MsaT023707.1:CDS
AGAAATGCTTGTGGGAAAGA+TGG 0.574345 4:-91604655 MsaT023707.1:CDS
ACTACATACCCAGCAAAATG+AGG 0.576860 4:+91605899 None:intergenic
TACCCTACACATTCATGACT+GGG 0.577744 4:+91602704 None:intergenic
GGGTTGAGTGATGCCAAAGA+TGG 0.578033 4:-91606321 MsaT023707.1:CDS
TTTACAACCATCAAGTGTAC+CGG 0.580118 4:+91603625 None:intergenic
TCTGCTAAGGAAGCTGTACA+TGG 0.580656 4:-91604730 MsaT023707.1:CDS
GGTGTTTGTGAAAACCTCGT+TGG 0.582249 4:+91603027 None:intergenic
GATGTTGTGCAGTAGCTCCA+AGG 0.582324 4:+91602916 None:intergenic
CGATTTGATAAAGCTGGAGG+AGG 0.582351 4:-91602564 MsaT023707.1:CDS
CCTGAATAATGGATGGCGAA+AGG 0.582584 4:+91602870 None:intergenic
ACCACGTGCAATAGAAGCGT+GGG 0.585756 4:-91604173 MsaT023707.1:CDS
CAATAAGTGTGTTCCATATG+CGG 0.587501 4:-91603762 MsaT023707.1:CDS
ATTTGTTATCACGTTTCCAA+GGG 0.587884 4:-91604905 MsaT023707.1:CDS
CATGGGACAAGGATGTCTTA+CGG 0.588015 4:+91604770 None:intergenic
GGTGGAGCCCAGCATGCTGG+AGG 0.588516 4:+91606633 None:intergenic
TTTCGAACATTAGTTGGACA+TGG 0.589138 4:+91603650 None:intergenic
GCCAAATCTACTCAACGAGT+TGG 0.589178 4:-91605263 MsaT023707.1:intron
TCATTAGTTCCTGTACAAGA+AGG 0.593267 4:+91604037 None:intergenic
ACACCTTATGTGGATCTGAC+TGG 0.594288 4:-91603926 MsaT023707.1:CDS
GCGTGGGAAACTAGAAACAT+TGG 0.596035 4:-91604157 MsaT023707.1:CDS
ATACAAATCTGAATCACTCT+CGG 0.596269 4:+91606040 None:intergenic
CATGGACAATAAAGTTAAGG+AGG 0.596567 4:-91603870 MsaT023707.1:CDS
CAACTTGAATGATACTATGT+TGG 0.596879 4:-91603894 MsaT023707.1:CDS
AAGACGGTTCCCGTCTCTCG+TGG 0.598519 4:+91606277 None:intergenic
AATAGATGAGCTAAGTACAT+TGG 0.598739 4:-91604215 MsaT023707.1:CDS
CACCTCAGCAGATAAGTGCT+GGG 0.599510 4:-91603008 MsaT023707.1:CDS
TGCTGGACAGTTCGATACAC+AGG 0.599561 4:+91604937 None:intergenic
GATCCCAGTCATGAATGTGT+AGG 0.603075 4:-91602707 MsaT023707.1:CDS
ATGTTGTGCAGTAGCTCCAA+GGG 0.604106 4:+91602917 None:intergenic
GCCGATGAGGAAGAAAAGTA+GGG 0.604404 4:-91606526 MsaT023707.1:CDS
GAATGATACTATGTTGGTCA+TGG 0.604927 4:-91603888 MsaT023707.1:CDS
TTCCCAGCACTTATCTGCTG+AGG 0.605988 4:+91603006 None:intergenic
AACATAGAACTTAGTGCTGG+TGG 0.608237 4:-91603701 MsaT023707.1:CDS
GACAACATAGAACTTAGTGC+TGG 0.610680 4:-91603704 MsaT023707.1:CDS
CACATTCCAACATACAGCCA+TGG 0.611126 4:+91605927 None:intergenic
CCTTCAATATCCTCCAAAGA+CGG 0.614825 4:+91606261 None:intergenic
GCCGCAGATATCATCCAGAT+GGG 0.615605 4:-91606884 MsaT023707.1:intron
AAAGGGCCAAGAAATCCAGC+AGG 0.615665 4:+91603215 None:intergenic
GAAACCGACTGATGAGGTCG+AGG 0.617162 4:-91606217 MsaT023707.1:intron
TATGCCATATGGTTCAGCTA+AGG 0.619568 4:+91606668 None:intergenic
GTGGAGCCCAGCATGCTGGA+GGG 0.623032 4:+91606634 None:intergenic
TTCAATACTCAAGTCTGAGG+AGG 0.624499 4:-91604962 MsaT023707.1:CDS
TTCAGAACCGAGAGCCGATG+AGG 0.624951 4:-91606539 MsaT023707.1:CDS
AACCACGTGCAATAGAAGCG+TGG 0.625238 4:-91604174 MsaT023707.1:CDS
AATAGAGAAACCGACTGATG+AGG 0.625485 4:-91606223 MsaT023707.1:CDS
TTGTGCAGAGGCTGTGAATG+TGG 0.627642 4:-91604851 MsaT023707.1:CDS
CTTGTACAGGAACTAATGAG+AGG 0.627759 4:-91604033 MsaT023707.1:CDS
TCCTTCAATACTCAAGTCTG+AGG 0.631456 4:-91604965 MsaT023707.1:CDS
TTCTCCTTAAGAGGGCAAGG+TGG 0.634638 4:+91606615 None:intergenic
ATTACCTCGACCTCATCAGT+CGG 0.637187 4:+91606213 None:intergenic
TGAGGAAGAAAAGTAGGGCA+AGG 0.637571 4:-91606521 MsaT023707.1:CDS
CATCTTCTATAGCTGAAGCA+TGG 0.639567 4:+91605323 None:intergenic
CAGTGGCTTCAATTGTGCAG+AGG 0.641990 4:-91604863 MsaT023707.1:CDS
TTAAATTGGAGAAATGCTTG+TGG 0.644063 4:-91604664 MsaT023707.1:CDS
GGGATCCAGTCGAAGCATGT+CGG 0.647902 4:-91606864 MsaT023707.1:CDS
AAGCTGGAGGAGGATGACAT+AGG 0.648499 4:-91602554 MsaT023707.1:CDS
TCAACTTGCTGAATACGGGT+CGG 0.649157 4:+91606567 None:intergenic
TCCCACGCTTCTATTGCACG+TGG 0.656740 4:+91604172 None:intergenic
CAGCTATAGAAGATGCAATG+AGG 0.660862 4:-91605315 MsaT023707.1:CDS
TAACAACTTATCATGGGACA+AGG 0.661705 4:+91604759 None:intergenic
GGTGACCCAAAAGTATGGTA+TGG 0.666509 4:-91605358 MsaT023707.1:CDS
TCGAACTGTCCAGCATTCTG+GGG 0.666808 4:-91604929 MsaT023707.1:CDS
TGTCGATTTGATAAAGCTGG+AGG 0.667838 4:-91602567 MsaT023707.1:CDS
CTGATGCACAATTGACTCAG+TGG 0.670313 4:-91602430 MsaT023707.1:CDS
ATAGCTCTCTAGGTGACTCG+AGG 0.683160 4:-91602604 MsaT023707.1:CDS
TTGTGCAGTAGCTCCAAGGG+AGG 0.695431 4:+91602920 None:intergenic
TCAAACGTTGGATAAAACAC+AGG 0.696828 4:+91606823 None:intergenic
AGGGCAAGGAAACGAAAACG+TGG 0.698161 4:-91606507 MsaT023707.1:CDS
TCACTTAATTATTTGCACTG+GGG 0.704148 4:-91605379 MsaT023707.1:CDS
CTGTTGTGTATGGAAAACAT+TGG 0.709730 4:-91603439 MsaT023707.1:CDS
TGAAGGAAGTGACATCTCAG+GGG 0.710950 4:-91605958 MsaT023707.1:CDS
AGCCACTGCTGAAGCCACAG+TGG 0.722140 4:+91604878 None:intergenic
TCTGAAGAAATGTGACTGTG+AGG 0.731640 4:+91603986 None:intergenic
TTTCCAAGGGCATACCACTG+TGG 0.743487 4:-91604892 MsaT023707.1:CDS
ACTCGCAACCAACAGCAGAG+AGG 0.744115 4:+91604326 None:intergenic
TGTTTGTGAAAACCTCGTTG+GGG 0.744486 4:+91603029 None:intergenic
TTTGGAAGAACACCCCAACG+AGG 0.753244 4:-91603041 MsaT023707.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 91602379 91607124 91602379 ID=MsaG023707
Chr4 mRNA 91602379 91607124 91602379 ID=MsaT023707.1;Parent=MsaG023707
Chr4 exon 91602379 91602744 91602379 ID=MsaT023707.1.exon10;Parent=MsaT023707.1
Chr4 CDS 91602379 91602744 91602379 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91602871 91603068 91602871 ID=MsaT023707.1.exon9;Parent=MsaT023707.1
Chr4 CDS 91602871 91603068 91602871 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91603208 91603311 91603208 ID=MsaT023707.1.exon8;Parent=MsaT023707.1
Chr4 CDS 91603208 91603311 91603208 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91603411 91604473 91603411 ID=MsaT023707.1.exon7;Parent=MsaT023707.1
Chr4 CDS 91603411 91604473 91603411 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91604633 91605001 91604633 ID=MsaT023707.1.exon6;Parent=MsaT023707.1
Chr4 CDS 91604633 91605001 91604633 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91605264 91605425 91605264 ID=MsaT023707.1.exon5;Parent=MsaT023707.1
Chr4 CDS 91605264 91605425 91605264 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91605908 91606129 91605908 ID=MsaT023707.1.exon4;Parent=MsaT023707.1
Chr4 CDS 91605908 91606129 91605908 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91606218 91606730 91606218 ID=MsaT023707.1.exon3;Parent=MsaT023707.1
Chr4 CDS 91606218 91606730 91606218 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91606822 91606899 91606822 ID=MsaT023707.1.exon2;Parent=MsaT023707.1
Chr4 CDS 91606822 91606899 91606822 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Chr4 exon 91607080 91607124 91607080 ID=MsaT023707.1.exon1;Parent=MsaT023707.1
Chr4 CDS 91607080 91607124 91607080 ID=cds.MsaT023707.1;Parent=MsaT023707.1
Gene Sequence

>MsaT023707.1

ATGGAAAATACACTTGAATCTTCCATCAATCCACAACACAAAAAGATATCATCCAGATGGGATCCAGTCGAAGCATGTCGGCCTATAATCGGTGAAACACCTGTGTTTTATCCAACGTTTGAGGAGTTTGAAGATACGCTTGGTTACCTAGCTAAGATACGTCCCTTAGCTGAACCATATGGCATATGTAAAATTGTCCCTCCAGCATGCTGGGCTCCACCTTGCCCTCTTAAGGAGAAAGAGATATGGGAAAATGCTAAATTTCCGACCCGTATTCAGCAAGTTGATTTGCTTCAGAACCGAGAGCCGATGAGGAAGAAAAGTAGGGCAAGGAAACGAAAACGTGGAAAGCAGTCAAAGATGGGAACGTGTAATAGGAGAACTGGAAATTCATGTTCTGAAGCTAATGCTACTTCTGAATCTGATGATAAGTTTGGATTCCAATCAGGGCCAGACTTCACACTTAAAGAATTTCAGCAATATGGAAATTCTTTTAAAGATTGCTACTTCGGGTTGAGTGATGCCAAAGATGGTAAAGGTAGTGATAATAATCACCACGAGAGACGGGAACCGTCTTTGGAGGATATTGAAGGTGAATACTGGCGAATAATAGAGAAACCGACTGATGAGGTCGAGGTGTATTATGGAGCTGACTTGGAAACTGGAGCACTTGGAAGTGGTTTTCCTAAGACATCATCCTTAACCGAGAGTGATTCAGATTTGTATGCTATGTCTGGCTGGAATTTGAATAACTTTGCACGACTTCCAGGTTCTGCATTATGTTTTGAAGGAAGTGACATCTCAGGGGTTTTAGTTCCATGGCTGTATGTTGGAATGTGCTTTTCCTCATTTTGCTGGCATGTTGAAGATCATCACCTCTATTCACTTAATTATTTGCACTGGGGTGACCCAAAAGTATGGTATGGTGTACCTGGCAGCCATGCTTCAGCTATAGAAGATGCAATGAGGAAACATTTGCCTGATTTGTTTGAAGAGCAGCCAAATCTACTCAACGAGTTGGTGACTCAGTTCTCTCCTTCAATACTCAAGTCTGAGGAGGTTCCTGTGTATCGAACTGTCCAGCATTCTGGGGAATTTGTTATCACGTTTCCAAGGGCATACCACTGTGGCTTCAGCAGTGGCTTCAATTGTGCAGAGGCTGTGAATGTGGCTCCATATGATTGGTTTATGCATGGTCAGAACGCTGCAGAGATTTACAGTCTACAGTGCCGTAAGACATCCTTGTCCCATGATAAGTTGTTATTTGGATCTGCTAAGGAAGCTGTACATGGCCTTTCAGAGACAACTCTTCATGGAAAAGAAAATCTAAAATGTTTAAATTGGAGAAATGCTTGTGGGAAAGATGGTGTTCTTACCAATGCAGTTAAGACAAGGATAATGATGGAAAAAGAGAGGCTTGAATGGCTTCCGGATCATTTAAAGATGCTGAAGATGGACAATGACTTCGATTCGGTTGAGGAAAGGGAATGTTACTATTGCTTCTATGACTTGCACCTCTCTGCTGTTGGTTGCGAGTGCTTTCCTGACAACTATTCTTGTCTTAGACATTTCAAGTTGTTTTGCTCATGTGAAATGGATAAAAGATTTGTTCTGGTTCGTTATACAATAGATGAGCTAAGTACATTGGTTGAAGCATTAGAAGGAAAACCACGTGCAATAGAAGCGTGGGAAACTAGAAACATTGGAGTTGTTTCTGCTAGTGTTGAGGATGCTTGCATGCACGAACAAGATATGGAGAGAGTCATGTGCAAAACTGAGAATTACAAAGAAGGGAAAAGATCGCCTTCTTGTACAGGAACTAATGAGAGGTTAAATTCAAATTTACCTAGCAGTCCTCACAGTCACATTTCTTCAGAGTTGGTCCATTCAGAGTCTCATCATGAAACTTCTCGTACACCTTATGTGGATCTGACTGGTGATATGGACAACTTGAATGATACTATGTTGGTCATGGACAATAAAGTTAAGGAGGATAAGGGAAGTTCTTTGTATTTGAATATTGGTGATATCTCTGATAAACCTGCAAATTCTTTGCTAAATATAGCCGAAATCCGTCACAATAAGTGTGTTCCATATGCGGAAAAAGTTACTAGTGCAGAGATCAGAAAAGAACGAGACAACATAGAACTTAGTGCTGGTGGTATTTCTGTCTTGGAAAATGAACCTTCACCATGTCCAACTAATGTTCGAAATTCCGGTACACTTGATGGTTGTAAATTGTTTGGTGTCAATCTGCAAATGCACTCAGATTTAGGACAGAAACTTAATAGTATGCTTGAAACGGGAGTTCTAGACACCTCCAATACAAGTATATCTTTGACAAACCAAAGCTCCCCAATGAAAAAGTTTAGTATTTCGGCTGAGCTTGTTAGTTTGGGATCTGTTGTGTATGGAAAACATTGGTGCAGTAAGCATGCAATATATCCAAAAGGATTCAAGAGTCGTGTAAAGTTCTTTAGCATTCTTGATCCATCAAGCATCTGTAACTATGTTTGTGAAGTCATTCCTGCTGGATTTCTTGGCCCTTTTTTCAGGGTTACTTTGGAAGAACACCCCAACGAGGTTTTCACAAACACCTCAGCAGATAAGTGCTGGGAATCTGTTATTGATAGACTAAACTATGAAATAAATAAGCGCAAGAGTCTCGGTGAACTAGACATGCCTCCCTTGGAGCTACTGCACAACATCAATGGTCATAAAATGTTTGGATTCCTTTCGCCATCCATTATTCAGTCCATTGAAGCTCAAGATCCCAGTCATGAATGTGTAGGGTACTGGAATCACAAACAAGTTAATTCTGGATCTTCAGGCAAAGCCATTGATGACTCTAAGTTAACTTGTGGTTCAAGTAATAGCTCTCTAGGTGACTCGAGGACCAAACTTTTTGGTGTCGATTTGATAAAGCTGGAGGAGGATGACATAGGAGAAAGTTCTCATTCGTTTGAAGAGATGAAGTTGATTCTAGAAAGATTCCTGAAAAAGGGAAGTCCTGACGAGTTACGAGCAATGCGTCAGTTATTCAGTTCTGATGCACAATTGACTCAGTGGAGACCTGCGCTGAAAGTGCTGATAGAGAAAATCCAGAAAGATTGTGAATAA
Protein sequence

>MsaT023707.1

MENTLESSINPQHKKISSRWDPVEACRPIIGETPVFYPTFEEFEDTLGYLAKIRPLAEPYGICKIVPPACWAPPCPLKEKEIWENAKFPTRIQQVDLLQNREPMRKKSRARKRKRGKQSKMGTCNRRTGNSCSEANATSESDDKFGFQSGPDFTLKEFQQYGNSFKDCYFGLSDAKDGKGSDNNHHERREPSLEDIEGEYWRIIEKPTDEVEVYYGADLETGALGSGFPKTSSLTESDSDLYAMSGWNLNNFARLPGSALCFEGSDISGVLVPWLYVGMCFSSFCWHVEDHHLYSLNYLHWGDPKVWYGVPGSHASAIEDAMRKHLPDLFEEQPNLLNELVTQFSPSILKSEEVPVYRTVQHSGEFVITFPRAYHCGFSSGFNCAEAVNVAPYDWFMHGQNAAEIYSLQCRKTSLSHDKLLFGSAKEAVHGLSETTLHGKENLKCLNWRNACGKDGVLTNAVKTRIMMEKERLEWLPDHLKMLKMDNDFDSVEERECYYCFYDLHLSAVGCECFPDNYSCLRHFKLFCSCEMDKRFVLVRYTIDELSTLVEALEGKPRAIEAWETRNIGVVSASVEDACMHEQDMERVMCKTENYKEGKRSPSCTGTNERLNSNLPSSPHSHISSELVHSESHHETSRTPYVDLTGDMDNLNDTMLVMDNKVKEDKGSSLYLNIGDISDKPANSLLNIAEIRHNKCVPYAEKVTSAEIRKERDNIELSAGGISVLENEPSPCPTNVRNSGTLDGCKLFGVNLQMHSDLGQKLNSMLETGVLDTSNTSISLTNQSSPMKKFSISAELVSLGSVVYGKHWCSKHAIYPKGFKSRVKFFSILDPSSICNYVCEVIPAGFLGPFFRVTLEEHPNEVFTNTSADKCWESVIDRLNYEINKRKSLGELDMPPLELLHNINGHKMFGFLSPSIIQSIEAQDPSHECVGYWNHKQVNSGSSGKAIDDSKLTCGSSNSSLGDSRTKLFGVDLIKLEEDDIGESSHSFEEMKLILERFLKKGSPDELRAMRQLFSSDAQLTQWRPALKVLIEKIQKDCE*