AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023809

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023809 MtrunA17_Chr8g0382191 92.486 1038 53 2 1 1013 47 1084 0.0 1986
MsaG023809 MtrunA17_Chr2g0279141 44.444 1062 464 28 2 1012 52 1038 0.0 761
MsaG023809 MtrunA17_Chr4g0050401 49.701 668 271 12 388 1012 382 1027 0.0 605
MsaG023809 MtrunA17_Chr4g0050401 37.295 244 119 6 2 231 52 275 4.75e-36 148
MsaG023809 MtrunA17_Chr8g0382181 61.774 327 108 5 5 318 8 330 2.13e-122 382
MsaG023809 MtrunA17_Chr8g0382181 69.863 146 39 1 410 550 330 475 8.64e-58 207
MsaG023809 MtrunA17_Chr8g0377501 42.701 548 269 15 450 967 402 934 6.74e-115 377
MsaG023809 MtrunA17_Chr8g0377501 48.077 156 62 4 2 151 53 195 5.93e-35 144
MsaG023809 MtrunA17_Chr3g0122351 39.252 535 296 7 455 969 386 911 1.05e-97 330
MsaG023809 MtrunA17_Chr3g0122351 70.238 84 25 0 2 85 46 129 1.56e-34 143
MsaG023809 MtrunA17_Chr4g0068161 32.407 648 369 17 386 1000 310 921 7.44e-83 288
MsaG023809 MtrunA17_Chr4g0068161 57.547 106 42 1 1 103 63 168 2.09e-25 113
MsaG023809 MtrunA17_Chr2g0294711 33.279 616 349 16 386 966 298 886 1.29e-80 281
MsaG023809 MtrunA17_Chr2g0294711 48.120 133 60 2 1 133 63 186 6.94e-31 131
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023809 AT2G22300.2 46.981 1060 421 29 2 1009 52 1022 0.0 827
MsaG023809 AT2G22300.1 46.981 1060 421 29 2 1009 52 1022 0.0 827
MsaG023809 AT2G22300.3 46.914 1053 418 29 9 1009 1 964 0.0 817
MsaG023809 AT5G64220.1 44.253 1044 463 30 2 994 52 1027 0.0 765
MsaG023809 AT5G64220.2 44.253 1044 463 30 2 994 52 1027 0.0 765
MsaG023809 AT5G09410.4 48.143 673 276 16 388 1009 231 881 0.0 587
MsaG023809 AT5G09410.3 48.143 673 276 16 388 1009 411 1061 0.0 584
MsaG023809 AT5G09410.3 53.247 154 40 3 2 125 114 265 1.21e-37 153
MsaG023809 AT5G09410.2 48.143 673 276 16 388 1009 352 1002 0.0 584
MsaG023809 AT5G09410.2 66.129 124 40 2 2 125 55 176 1.33e-42 169
MsaG023809 AT5G09410.1 48.143 673 276 16 388 1009 334 984 0.0 583
MsaG023809 AT5G09410.1 66.129 124 40 2 2 125 55 176 1.32e-42 169
MsaG023809 AT1G67310.1 39.383 551 283 11 453 967 442 977 1.17e-98 334
MsaG023809 AT1G67310.1 53.571 112 42 1 2 113 75 176 1.83e-32 137
MsaG023809 AT3G16940.3 31.648 613 359 14 386 966 217 801 1.22e-75 266
MsaG023809 AT3G16940.3 59.341 91 37 0 1 91 34 124 6.88e-29 125
MsaG023809 AT3G16940.1 31.648 613 359 14 386 966 244 828 1.43e-75 266
MsaG023809 AT3G16940.1 59.341 91 37 0 1 91 61 151 7.30e-29 125
MsaG023809 AT3G16940.2 30.268 522 314 12 386 883 244 739 4.70e-53 199
MsaG023809 AT3G16940.2 59.341 91 37 0 1 91 61 151 4.81e-29 125

Find 205 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGTTATTGGGTTCTGTTTC+AGG 0.110677 4:+92598965 None:intergenic
TCCAACATTGTTAATGTATT+TGG 0.116953 4:+92599084 None:intergenic
GATAGGGGACAGTTAGAATA+AGG 0.169692 4:+92599515 None:intergenic
ATTCCCCTTCTAGTGCTTAT+TGG 0.214001 4:-92597985 MsaT023809.1:CDS
GAATACGATGGTGACAAATT+TGG 0.239421 4:-92595955 MsaT023809.1:CDS
GAAATTTACCTTTGAGAGAT+AGG 0.252450 4:+92599498 None:intergenic
ACTATTGATTCCCGTGTTAT+TGG 0.255860 4:+92598952 None:intergenic
TGTGAACTCTCAAAAGTTCT+TGG 0.257449 4:-92599055 MsaT023809.1:CDS
TCTACGTAACATGTTCTAAT+AGG 0.258394 4:-92597406 MsaT023809.1:CDS
GCAAGATATCTTAGAAAATA+AGG 0.263006 4:-92599020 MsaT023809.1:CDS
GCCTGAATAGGCACTATCTT+TGG 0.278496 4:+92598336 None:intergenic
TTCCCCTTCTAGTGCTTATT+GGG 0.285574 4:-92597984 MsaT023809.1:CDS
AGCAACAATTGTTGGTTCTA+AGG 0.288908 4:+92596952 None:intergenic
ATTTGAAGACTCGGAAATTA+AGG 0.297823 4:-92597828 MsaT023809.1:intron
AAAACTATGGGAAGATAATT+TGG 0.298279 4:-92595618 MsaT023809.1:CDS
CCACCTTCATCTAATACATT+TGG 0.299243 4:+92597019 None:intergenic
GTGAACTTTCGCGATGTAAA+TGG 0.307864 4:-92596920 MsaT023809.1:CDS
ATACCTTCACTTCCCGATAA+TGG 0.312933 4:+92600127 None:intergenic
TGAAACTGTCGAGCTTCTTC+AGG 0.320899 4:+92598056 None:intergenic
CGTTGATCTTGAAGCATTGT+TGG 0.336518 4:-92594961 MsaT023809.1:CDS
TTGCTTCCCTCATATCTCTT+GGG 0.341997 4:-92596305 MsaT023809.1:CDS
CAGCCTTTCTTGGCTACGAT+TGG 0.345539 4:-92596976 MsaT023809.1:CDS
CCCAAAGATAGTGCCTATTC+AGG 0.346532 4:-92598337 MsaT023809.1:CDS
GATACAGTTGAAAGCGAAAA+TGG 0.348848 4:-92597962 MsaT023809.1:CDS
CAGCTGAAGCTGAAGGAAAT+TGG 0.351228 4:-92598850 MsaT023809.1:CDS
GACAAGTTAAAATCAGATCT+AGG 0.361350 4:-92598163 MsaT023809.1:CDS
CACCTTCATCTAATACATTT+GGG 0.362638 4:+92597020 None:intergenic
GGCATGCATTAAAGTTGCTT+TGG 0.362966 4:+92599906 None:intergenic
GGCTATTTCTGAGGGAACTA+TGG 0.368661 4:-92595521 MsaT023809.1:CDS
ATGAAGCAGAAGATTGTAAA+TGG 0.369204 4:-92597526 MsaT023809.1:CDS
GTCGGTTGGGATTGTGGAAA+AGG 0.373416 4:-92595596 MsaT023809.1:CDS
GTTGCTTCCCTCATATCTCT+TGG 0.375690 4:-92596306 MsaT023809.1:CDS
CTATTGATTCCCGTGTTATT+GGG 0.377443 4:+92598953 None:intergenic
ATTCAATAGCCATGCAAATT+CGG 0.381684 4:-92599597 MsaT023809.1:CDS
AGCTGATCATAGCAAATAGA+TGG 0.386884 4:+92597884 None:intergenic
TGAAATTGTGATAGATGATC+AGG 0.393656 4:-92599164 MsaT023809.1:intron
ATGGATGGACTGCTCTTCAT+TGG 0.403464 4:-92596901 MsaT023809.1:CDS
ACCAAATACATTAACAATGT+TGG 0.404096 4:-92599085 MsaT023809.1:CDS
TGGTATTGGTCTCTTGCATC+TGG 0.406003 4:+92595383 None:intergenic
CCATGCCCACAACATCCTTC+TGG 0.406110 4:-92596258 MsaT023809.1:CDS
TTAGAAAAGATGGACATAAC+TGG 0.406867 4:-92601199 MsaT023809.1:CDS
GGAAGATAATTTGGTCGGTT+GGG 0.407139 4:-92595609 MsaT023809.1:CDS
CATTCAACAACCTGTGGTAT+TGG 0.408725 4:+92595369 None:intergenic
ACGTGAGAGGTCACCAGGTT+AGG 0.411787 4:-92595642 MsaT023809.1:CDS
ACATCGTTCTAGTCCATTAT+CGG 0.412410 4:-92600140 MsaT023809.1:CDS
GTATCCCAATAAGCACTAGA+AGG 0.413133 4:+92597980 None:intergenic
AATTTGGTCGGTTGGGATTG+TGG 0.413549 4:-92595602 MsaT023809.1:CDS
GCCGACCTAGCTTCTGCAAA+TGG 0.416041 4:-92596225 MsaT023809.1:CDS
TTTCCTTGTGAACAGGCCTC+TGG 0.421100 4:-92598388 MsaT023809.1:intron
TTCACACCAGCAACAATTGT+TGG 0.422507 4:+92596944 None:intergenic
CTTCAGAAAACAACTGAAGA+TGG 0.424083 4:-92597049 MsaT023809.1:CDS
TTAGAACCAACAATTGTTGC+TGG 0.425908 4:-92596950 MsaT023809.1:CDS
CCTGAATAGGCACTATCTTT+GGG 0.427656 4:+92598337 None:intergenic
CATAGTTCTCCAAGTGCACC+TGG 0.428531 4:+92597915 None:intergenic
AACTGAGATACAAGAAAATC+AGG 0.429890 4:-92595342 MsaT023809.1:intron
GAATATGAAGAAGCTGAATC+AGG 0.434942 4:-92599775 MsaT023809.1:intron
CATTGATTATTCCCCAAGTT+GGG 0.435352 4:-92597852 MsaT023809.1:CDS
ATAGGGGACAGTTAGAATAA+GGG 0.437858 4:+92599516 None:intergenic
GGGAAGATAATTTGGTCGGT+TGG 0.442560 4:-92595610 MsaT023809.1:CDS
GCCAGTGAATCTTTCATTGA+AGG 0.442930 4:+92596060 None:intergenic
CAATTTCCTTCAGCTTCAGC+TGG 0.443285 4:+92598851 None:intergenic
CGTGTTGCAGCAACATGAAC+AGG 0.444655 4:+92595859 None:intergenic
AAATTTACCTTTGAGAGATA+GGG 0.446563 4:+92599499 None:intergenic
AGTTGATCATGTACAAGTTC+TGG 0.447402 4:+92597112 None:intergenic
AATGCGAAGTCACACAAGTC+TGG 0.449069 4:-92595895 MsaT023809.1:CDS
TCATTGATTATTCCCCAAGT+TGG 0.450073 4:-92597853 MsaT023809.1:CDS
TATTCCTTCCCAGGTGCACT+TGG 0.450888 4:-92597924 MsaT023809.1:CDS
ACAACAGAGAACACTATTCC+TGG 0.452005 4:+92597463 None:intergenic
AGCCTTTCTTGGCTACGATT+GGG 0.452371 4:-92596975 MsaT023809.1:CDS
TGTAAATGGTCATGCATGTT+CGG 0.458202 4:-92597512 MsaT023809.1:CDS
GAGTCTTCAAATGCCCAACT+TGG 0.460974 4:+92597839 None:intergenic
AGTAAAATCAGTTCTTTGCT+TGG 0.461341 4:-92597193 MsaT023809.1:CDS
CAGTTCTTTGCTTGGTAGGA+AGG 0.462643 4:-92597185 MsaT023809.1:CDS
TTAGAACATGTTACGTAGAA+AGG 0.466234 4:+92597410 None:intergenic
AATGGACACAAAGGAATTGC+TGG 0.466727 4:-92596207 MsaT023809.1:CDS
ATATTACGTTGGCGTCGAAA+AGG 0.469061 4:-92595571 MsaT023809.1:CDS
ACGAGATACTTTAGAAAAGA+TGG 0.469912 4:-92601209 MsaT023809.1:CDS
GAAGTCTCTGCCTCTGTGTT+AGG 0.470588 4:+92599832 None:intergenic
ACCTCAACACAAGGCTGGAC+GGG 0.474522 4:-92597435 MsaT023809.1:CDS
TCTCAAGGATATGAGACAAT+GGG 0.476448 4:-92598926 MsaT023809.1:CDS
CCCGAGGGAAGTCATGCTCT+TGG 0.481194 4:+92597257 None:intergenic
ATATCTCTTGGGGCAGCACC+AGG 0.481476 4:-92596294 MsaT023809.1:CDS
ATTCCAGAGAAAACAACAAA+AGG 0.481483 4:-92595977 MsaT023809.1:CDS
CCCAAGAGCATGACTTCCCT+CGG 0.481534 4:-92597258 MsaT023809.1:CDS
TGGATGGACTGCTCTTCATT+GGG 0.482883 4:-92596900 MsaT023809.1:CDS
CCGAGGGAAGTCATGCTCTT+GGG 0.487143 4:+92597258 None:intergenic
GGGATCGATAAAAGCCTAGT+TGG 0.487311 4:-92598142 MsaT023809.1:CDS
CTCACACGATGAGCCTGAAT+AGG 0.489940 4:+92598324 None:intergenic
CTAGTTCTTTACTCATCCAT+CGG 0.491817 4:+92598032 None:intergenic
GGCTCACGTGAGAGGTCACC+AGG 0.496633 4:-92595647 MsaT023809.1:CDS
GTTTCAGGAAAAGACTGAAA+AGG 0.498768 4:+92598980 None:intergenic
TGCTTCCCTCATATCTCTTG+GGG 0.499217 4:-92596304 MsaT023809.1:CDS
TTGGTAGGAAGGACGATGAA+TGG 0.501312 4:-92597174 MsaT023809.1:CDS
AAATCAGTTCTTTGCTTGGT+AGG 0.505479 4:-92597189 MsaT023809.1:CDS
CGTTGGCGTCGAAAAGGTAG+TGG 0.510926 4:-92595565 MsaT023809.1:CDS
TTCAGAACAAGTTTCGCAGT+TGG 0.512320 4:-92595834 MsaT023809.1:CDS
AGTCTTCAAATGCCCAACTT+GGG 0.512638 4:+92597840 None:intergenic
CCAGAAGGATGTTGTGGGCA+TGG 0.516822 4:+92596258 None:intergenic
ACCTTCAATGAAAGATTCAC+TGG 0.517231 4:-92596061 MsaT023809.1:CDS
TTTGTTGGTGTGTATGAAGA+AGG 0.517240 4:+92598236 None:intergenic
AAGGTTGCCTGTCATTCCTC+AGG 0.518272 4:-92599140 MsaT023809.1:CDS
CATGGATCAGTAAGTGCTCC+TGG 0.519816 4:+92596276 None:intergenic
TGGTAGGAAGGACGATGAAT+GGG 0.520017 4:-92597173 MsaT023809.1:CDS
CAGTACAACTATTCCTTCCC+AGG 0.520858 4:-92597933 MsaT023809.1:CDS
GAACAAGTTTCGCAGTTGGA+AGG 0.521020 4:-92595830 MsaT023809.1:CDS
CATCGTTCTAGTCCATTATC+GGG 0.523240 4:-92600139 MsaT023809.1:CDS
TGTGTCCATTTGCAGAAGCT+AGG 0.527068 4:+92596220 None:intergenic
GGCCCAATCGTAGCCAAGAA+AGG 0.527167 4:+92596973 None:intergenic
GATATCTTAGAAAATAAGGC+TGG 0.527732 4:-92599016 MsaT023809.1:CDS
TAGTTATAGTGACAATGAGC+AGG 0.528100 4:-92598299 MsaT023809.1:CDS
ACAAGTTAAAATCAGATCTA+GGG 0.529700 4:-92598162 MsaT023809.1:CDS
TTCAGAAAACAACTGAAGAT+GGG 0.530144 4:-92597048 MsaT023809.1:CDS
AAGTGCCGGCAGAGATAACC+AGG 0.530702 4:-92597481 MsaT023809.1:CDS
TTCTCCAAGTGCACCTGGGA+AGG 0.531264 4:+92597920 None:intergenic
CTATTCCTGGTTATCTCTGC+CGG 0.532774 4:+92597476 None:intergenic
GGCCCAAATGTATTAGATGA+AGG 0.533133 4:-92597022 MsaT023809.1:CDS
GCACTTGGAGAACTATGTAC+TGG 0.534994 4:-92597909 MsaT023809.1:CDS
TTCTACCAGAAGGATGTTGT+GGG 0.536843 4:+92596253 None:intergenic
CAAATCAGAGGCTATTTCTG+AGG 0.541683 4:-92595530 MsaT023809.1:CDS
GTGCTGCCCCAAGAGATATG+AGG 0.541703 4:+92596298 None:intergenic
AACAAGTTTCGCAGTTGGAA+GGG 0.542396 4:-92595829 MsaT023809.1:CDS
CACCTCAACACAAGGCTGGA+CGG 0.542743 4:-92597436 MsaT023809.1:CDS
AGCTCGACAGTTTCAACCGA+TGG 0.542906 4:-92598048 MsaT023809.1:CDS
ACCCGTCCAGCCTTGTGTTG+AGG 0.545426 4:+92597434 None:intergenic
TCATCCACCTGAGGAATGAC+AGG 0.545608 4:+92599133 None:intergenic
GAGTGTCTTCTATTCCAACT+AGG 0.550195 4:+92598128 None:intergenic
AGTGTATCTGTGGCAGAAGA+GGG 0.550559 4:-92598079 MsaT023809.1:CDS
TTTGCAGAAGCTAGGTCGGC+TGG 0.551088 4:+92596228 None:intergenic
TGAAGCTGAAGGAAATTGGC+AGG 0.551960 4:-92598846 MsaT023809.1:intron
TGGAAAAGGTTATATTACGT+TGG 0.554077 4:-92595582 MsaT023809.1:CDS
TATCCCAATAAGCACTAGAA+GGG 0.554536 4:+92597981 None:intergenic
AATTTACCTTTGAGAGATAG+GGG 0.555076 4:+92599500 None:intergenic
CGATGGATGAGTAAAGAACT+AGG 0.555800 4:-92598031 MsaT023809.1:CDS
AAGTGTATCTGTGGCAGAAG+AGG 0.556458 4:-92598080 MsaT023809.1:CDS
TTTATACCAGCTGAAGCTGA+AGG 0.559293 4:-92598857 MsaT023809.1:CDS
GCAGGAAGCAAACTGAGAAA+AGG 0.561949 4:-92595448 MsaT023809.1:CDS
GTATTAGATGAAGGTGGCCA+AGG 0.562370 4:-92597013 MsaT023809.1:CDS
AAATCAGAGGCTATTTCTGA+GGG 0.564813 4:-92595529 MsaT023809.1:CDS
TTGTATGTTTGTTGATCACA+AGG 0.569291 4:+92599862 None:intergenic
AAGTTGGGCATTTGAAGACT+CGG 0.569405 4:-92597837 MsaT023809.1:CDS
CTGCAAAATGAAGCACACCT+TGG 0.570206 4:+92596996 None:intergenic
TCCATTTGCAGAAGCTAGGT+CGG 0.576737 4:+92596224 None:intergenic
GTCTTCAAATGCCCAACTTG+GGG 0.578425 4:+92597841 None:intergenic
TATACACCTCAACACAAGGC+TGG 0.578694 4:-92597440 MsaT023809.1:CDS
ATACTGTTTAGTAATGCTGA+TGG 0.578753 4:+92598892 None:intergenic
GCTAATTTCTAAGTGGCTGT+AGG 0.578993 4:+92594921 None:intergenic
AACTGTCCCCTATCTCTCAA+AGG 0.580934 4:-92599506 MsaT023809.1:intron
GGCACTATCTTTGGGCCATG+TGG 0.581003 4:+92598345 None:intergenic
GGAATCAATAGTACTTCTCA+AGG 0.582466 4:-92598941 MsaT023809.1:CDS
CCAAGAGCATGACTTCCCTC+GGG 0.583296 4:-92597257 MsaT023809.1:CDS
GTTCGGCGAGATAGAAGTGC+CGG 0.585098 4:-92597495 MsaT023809.1:CDS
CTATGGGAAGATAATTTGGT+CGG 0.585590 4:-92595614 MsaT023809.1:CDS
TCATACACACCAACAAAACA+AGG 0.587677 4:-92598230 MsaT023809.1:CDS
GAAGCAAACTGAGAAAAGGT+TGG 0.588658 4:-92595444 MsaT023809.1:CDS
TTCTCAAGGATATGAGACAA+TGG 0.588805 4:-92598927 MsaT023809.1:CDS
TGCTGCCCCAAGAGATATGA+GGG 0.589348 4:+92596299 None:intergenic
GTTCTACCAGAAGGATGTTG+TGG 0.592131 4:+92596252 None:intergenic
TTATTATAGGCTCACGTGAG+AGG 0.593673 4:-92595655 MsaT023809.1:intron
AGTAGAAATCCGAATTTGCA+TGG 0.594305 4:+92599588 None:intergenic
TAAAAGATAAACTACATGCA+TGG 0.594674 4:-92597075 MsaT023809.1:CDS
CCATTGACTTCACTCTAGCA+AGG 0.595945 4:+92595414 None:intergenic
TTGCACTGCTACTACGCCCA+TGG 0.597003 4:-92600880 MsaT023809.1:CDS
CGAAAAGGTAGTGGTTTGCG+TGG 0.599858 4:-92595556 MsaT023809.1:CDS
CATTGACTTCACTCTAGCAA+GGG 0.600713 4:+92595415 None:intergenic
CCTTGCTAGAGTGAAGTCAA+TGG 0.603870 4:-92595414 MsaT023809.1:CDS
TTGTTATACACCTCAACACA+AGG 0.604166 4:-92597444 MsaT023809.1:CDS
ATTCTTTAAGCGCATCCACA+TGG 0.606620 4:-92598360 MsaT023809.1:CDS
AGAAACATGAGAGAAACCGT+TGG 0.609159 4:-92596132 MsaT023809.1:CDS
GACTCTATTAAGTGTATCTG+TGG 0.611735 4:-92598089 MsaT023809.1:CDS
ATTGCTGCTTCTGATCTCAC+CGG 0.611990 4:+92597321 None:intergenic
ACTCAAGAAGTCAACACTGC+CGG 0.614091 4:-92597340 MsaT023809.1:intron
AGTCCATTATCGGGAAGTGA+AGG 0.615277 4:-92600130 MsaT023809.1:intron
TCGGCTGGTGTTCTACCAGA+AGG 0.617785 4:+92596243 None:intergenic
TTGTGATAGATGATCAGGAA+AGG 0.618020 4:-92599159 MsaT023809.1:intron
GTTGCCTGTCATTCCTCAGG+TGG 0.619752 4:-92599137 MsaT023809.1:CDS
CTATTCAGGCTCATCGTGTG+AGG 0.626917 4:-92598323 MsaT023809.1:CDS
ATGAAGAAGGAACTGTTCCA+AGG 0.628051 4:+92598249 None:intergenic
ACTTTCGCGATGTAAATGGA+TGG 0.628820 4:-92596916 MsaT023809.1:CDS
TCTGAGGGAACTATGGTACA+AGG 0.629731 4:-92595514 MsaT023809.1:intron
GATGGACGCTAATTTCTAAG+TGG 0.633815 4:+92594914 None:intergenic
TGACCACTGTTAATGCTAGA+CGG 0.638659 4:+92599805 None:intergenic
ATCCCAATAAGCACTAGAAG+GGG 0.640484 4:+92597982 None:intergenic
GCTTCTGCAAATGGACACAA+AGG 0.643597 4:-92596216 MsaT023809.1:CDS
GGAAGCTCATGAGAGACTCA+AGG 0.645990 4:-92601147 MsaT023809.1:intron
TGAAACAGAACCCAATAACA+CGG 0.650306 4:-92598963 MsaT023809.1:CDS
AAAACAACTGAAGATGGGAA+AGG 0.651527 4:-92597043 MsaT023809.1:CDS
TAATCGAATCTAAATCCCGA+GGG 0.653442 4:+92597242 None:intergenic
CCAAATGTATTAGATGAAGG+TGG 0.656043 4:-92597019 MsaT023809.1:CDS
ATGCAAGAGACCAATACCAC+AGG 0.658465 4:-92595379 MsaT023809.1:CDS
CTAATCGAATCTAAATCCCG+AGG 0.658480 4:+92597241 None:intergenic
GAAACAGAACCCAATAACAC+GGG 0.662228 4:-92598962 MsaT023809.1:CDS
ACGAACTTACTGGATGCTTG+AGG 0.663844 4:-92600833 MsaT023809.1:CDS
TTACCGTCTAGCATTAACAG+TGG 0.677007 4:-92599808 MsaT023809.1:CDS
GAAAAGATGGACATAACTGG+AGG 0.677852 4:-92601196 MsaT023809.1:CDS
ATGGACTGCTCTTCATTGGG+CGG 0.678377 4:-92596897 MsaT023809.1:CDS
AAACAACAAAAGGAATACGA+TGG 0.685779 4:-92595967 MsaT023809.1:CDS
TTTGCGTGGATTCAAATCAG+AGG 0.685933 4:-92595542 MsaT023809.1:CDS
TTACAACATTCAACAACCTG+TGG 0.686006 4:+92595363 None:intergenic
ATAGTTCTCCAAGTGCACCT+GGG 0.691663 4:+92597916 None:intergenic
CTCAAGGATATGAGACAATG+GGG 0.695507 4:-92598925 MsaT023809.1:CDS
GCTTAAAGAATTAAAACCAG+AGG 0.714724 4:+92598372 None:intergenic
CAAAGTTATGCCTAACACAG+AGG 0.717841 4:-92599842 MsaT023809.1:CDS
TTAGCATGTAGTGAAGTGCG+TGG 0.726926 4:-92597383 MsaT023809.1:intron
AGTAAGATATCATCCACCTG+AGG 0.729515 4:+92599124 None:intergenic
ACAATGCTTCAAGATCAACG+AGG 0.891821 4:+92594964 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 92594929 92601381 92594929 ID=MsaG023809
Chr4 mRNA 92594929 92601381 92594929 ID=MsaT023809.1;Parent=MsaG023809
Chr4 exon 92594929 92595039 92594929 ID=MsaT023809.1.exon14;Parent=MsaT023809.1
Chr4 CDS 92594929 92595039 92594929 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92595343 92595473 92595343 ID=MsaT023809.1.exon13;Parent=MsaT023809.1
Chr4 CDS 92595343 92595473 92595343 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92595515 92595668 92595515 ID=MsaT023809.1.exon12;Parent=MsaT023809.1
Chr4 CDS 92595515 92595668 92595515 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92595783 92596338 92595783 ID=MsaT023809.1.exon11;Parent=MsaT023809.1
Chr4 CDS 92595783 92596338 92595783 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92596884 92597352 92596884 ID=MsaT023809.1.exon10;Parent=MsaT023809.1
Chr4 CDS 92596884 92597352 92596884 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92597384 92597582 92597384 ID=MsaT023809.1.exon9;Parent=MsaT023809.1
Chr4 CDS 92597384 92597582 92597384 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92597829 92598395 92597829 ID=MsaT023809.1.exon8;Parent=MsaT023809.1
Chr4 CDS 92597829 92598395 92597829 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92598847 92599172 92598847 ID=MsaT023809.1.exon7;Parent=MsaT023809.1
Chr4 CDS 92598847 92599172 92598847 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92599507 92599620 92599507 ID=MsaT023809.1.exon6;Parent=MsaT023809.1
Chr4 CDS 92599507 92599620 92599507 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92599776 92599932 92599776 ID=MsaT023809.1.exon5;Parent=MsaT023809.1
Chr4 CDS 92599776 92599932 92599776 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92600131 92600173 92600131 ID=MsaT023809.1.exon4;Parent=MsaT023809.1
Chr4 CDS 92600131 92600173 92600131 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92600832 92600920 92600832 ID=MsaT023809.1.exon3;Parent=MsaT023809.1
Chr4 CDS 92600832 92600920 92600832 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92601148 92601263 92601148 ID=MsaT023809.1.exon2;Parent=MsaT023809.1
Chr4 CDS 92601148 92601263 92601148 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Chr4 exon 92601372 92601381 92601372 ID=MsaT023809.1.exon1;Parent=MsaT023809.1
Chr4 CDS 92601372 92601381 92601372 ID=cds.MsaT023809.1;Parent=MsaT023809.1
Gene Sequence

>MsaT023809.1

ATGCCTCCAAGTGGTTCACTTTTCTTGTTTGATCGAAAGGTGACGAGATACTTTAGAAAAGATGGACATAACTGGAGGAAGAAAAAAGATGGAAAAACTGTTAGGGAAGCTCATGAGAGACTCAAGGCTGGAAGTGTGGAAGTATTGCACTGCTACTACGCCCATGGAGAACAAAATGACAATTTTCAAAGACGAACTTACTGGATGCTTGAGGAGGAACTCTCACACATCGTTCTAGTCCATTATCGGGAAGTGAAGGGAACCAAAGCAACTTTAATGCATGCCAAAGAAAATGAAGAATCTAATCCTTGTGATCAACAAACATACAAAGTTATGCCTAACACAGAGGCAGAGACTTCTTTACCGTCTAGCATTAACAGTGGTCAAGCATCAGAATATGAAGAAGCTGAATCAGCATTCAATAGCCATGCAAATTCGGATTTCTACTCTTTTCTTGAGCTGCAACAGCCTGCTGTTCAAAAGATTAAAGCTCAACTCCCTTATTCTAACTGTCCCCTATCTCTCAAAGATGATCAGGAAAGGTTGCCTGTCATTCCTCAGGTGGATGATATCTTACTCAGTCAAGCTAATGAAACCAAATACATTAACAATGTTGGATTGACATGTGAACTCTCAAAAGTTCTTGGCTTTTCATCATGGCAAGATATCTTAGAAAATAAGGCTGGAAGTCATAATGTGCCTTTTCAGTCTTTTCCTGAAACAGAACCCAATAACACGGGAATCAATAGTACTTCTCAAGGATATGAGACAATGGGGCAACATTTAACCATCAGCATTACTAAACAGTATGATAATAGAAGTTTTATACCAGCTGAAGCTGAAGGAAATTGGCAGGCCTCTGGTTTTAATTCTTTAAGCGCATCCACATGGCCCAAAGATAGTGCCTATTCAGGCTCATCGTGTGAGGTTAGTTATAGTGACAATGAGCAGGAAGTTAATGAAGTTGATTTTCAACAATCCTTGGAACAGTTCCTTCTTCATACACACCAACAAAACAAGGTTCTTATGCAAAATAGCCCTCATGAAATACTTTTAAAAGCAGAAGACAAGTTAAAATCAGATCTAGGGATCGATAAAAGCCTAGTTGGAATAGAAGACACTCATTTCACTTCAAAGAAGACTCTATTAAGTGTATCTGTGGCAGAAGAGGGCCTGAAGAAGCTCGACAGTTTCAACCGATGGATGAGTAAAGAACTAGGCGATGTCGAAGAATCAAGCAAGCATTCCCCTTCTAGTGCTTATTGGGATACAGTTGAAAGCGAAAATGGTGTTGACAGTACAACTATTCCTTCCCAGGTGCACTTGGAGAACTATGTACTGGATCCATCTATTTGCTATGATCAGCTTTTTAGCATCATTGATTATTCCCCAAGTTGGGCATTTGAAGACTCGGAAATTAAGGTTCTCATTTCAGGACGATTCTTAAAGAGTCAACATGAAGCAGAAGATTGTAAATGGTCATGCATGTTCGGCGAGATAGAAGTGCCGGCAGAGATAACCAGGAATAGTGTTCTCTGTTGTTATACACCTCAACACAAGGCTGGACGGGTTCCTTTCTACGTAACATGTTCTAATAGGTTAGCATGTAGTGAAGTGCGTGTCAACACTGCCGGTGAGATCAGAAGCAGCAATACTTATGATACTTTTAATAAACGATTTGGAGAATTGTTGTCCCAAGAGCATGACTTCCCTCGGGATTTAGATTCGATTAGTGTGAATGAGAAATCTCAACTGAGAAGTAAAATCAGTTCTTTGCTTGGTAGGAAGGACGATGAATGGGACGAACTACTTAAATTTACTCTAGACAAAGATTTTTCTCCAGAACTTGTACATGATCAACTGCTTCAAAATCTTGTAAAAGATAAACTACATGCATGGCTCCTTCAGAAAACAACTGAAGATGGGAAAGGCCCAAATGTATTAGATGAAGGTGGCCAAGGTGTGCTTCATTTTGCAGCCTTTCTTGGCTACGATTGGGCCTTAGAACCAACAATTGTTGCTGGTGTGAATGTGAACTTTCGCGATGTAAATGGATGGACTGCTCTTCATTGGGCGGCGTTTTGTGGCAGGGAGAGGACAGTTGCTTCCCTCATATCTCTTGGGGCAGCACCAGGAGCACTTACTGATCCATGCCCACAACATCCTTCTGGTAGAACACCAGCCGACCTAGCTTCTGCAAATGGACACAAAGGAATTGCTGGTTATCTTGCAGAATCTTTCTTAAGCTCACAACTCAAGTCTCTTGATTTGAAAAGAAACATGAGAGAAACCGTTGGAACAAAACAAAGAGTCCGAGAACAAAACAATGAGTGCTTCTCACATGAACCTTCAATGAAAGATTCACTGGCTGCAGTGTGCAATGCAACTCAAGCTGCTGCTCGTATTCATCAAGTTTTTAGAGTGCAATCATTCCAGAGAAAACAACAAAAGGAATACGATGGTGACAAATTTGGAATATCTGATGAACGTGCTCTTTCACTAATATCAGTGAATGCGAAGTCACACAAGTCTGGACTACGCAATGAGCCTGTTCATGTTGCTGCAACACGTATTCAGAACAAGTTTCGCAGTTGGAAGGGCAGAAAAGATTTTTTGATTATCCGACAGCGAATTGTAAAAATTCAGGCTCACGTGAGAGGTCACCAGGTTAGGAAAAACTATGGGAAGATAATTTGGTCGGTTGGGATTGTGGAAAAGGTTATATTACGTTGGCGTCGAAAAGGTAGTGGTTTGCGTGGATTCAAATCAGAGGCTATTTCTGAGGGAACTATGGTACAAGAAGGCAGGAAGCAAACTGAGAAAAGGTTGGAGAAAGCCCTTGCTAGAGTGAAGTCAATGGCTCAATATCCAGATGCAAGAGACCAATACCACAGGTTGTTGAATGTTGTAACTGAGATACAAGAAAATCAGGTAAAGCAAGATCGAAATTTTATCAATTCAGAAGAAACAAGACAATTCAATAACCTCGTTGATCTTGAAGCATTGTTGGACGAAGATACTTTCATGCCTACAGCCACTTAG
Protein sequence

>MsaT023809.1

MPPSGSLFLFDRKVTRYFRKDGHNWRKKKDGKTVREAHERLKAGSVEVLHCYYAHGEQNDNFQRRTYWMLEEELSHIVLVHYREVKGTKATLMHAKENEESNPCDQQTYKVMPNTEAETSLPSSINSGQASEYEEAESAFNSHANSDFYSFLELQQPAVQKIKAQLPYSNCPLSLKDDQERLPVIPQVDDILLSQANETKYINNVGLTCELSKVLGFSSWQDILENKAGSHNVPFQSFPETEPNNTGINSTSQGYETMGQHLTISITKQYDNRSFIPAEAEGNWQASGFNSLSASTWPKDSAYSGSSCEVSYSDNEQEVNEVDFQQSLEQFLLHTHQQNKVLMQNSPHEILLKAEDKLKSDLGIDKSLVGIEDTHFTSKKTLLSVSVAEEGLKKLDSFNRWMSKELGDVEESSKHSPSSAYWDTVESENGVDSTTIPSQVHLENYVLDPSICYDQLFSIIDYSPSWAFEDSEIKVLISGRFLKSQHEAEDCKWSCMFGEIEVPAEITRNSVLCCYTPQHKAGRVPFYVTCSNRLACSEVRVNTAGEIRSSNTYDTFNKRFGELLSQEHDFPRDLDSISVNEKSQLRSKISSLLGRKDDEWDELLKFTLDKDFSPELVHDQLLQNLVKDKLHAWLLQKTTEDGKGPNVLDEGGQGVLHFAAFLGYDWALEPTIVAGVNVNFRDVNGWTALHWAAFCGRERTVASLISLGAAPGALTDPCPQHPSGRTPADLASANGHKGIAGYLAESFLSSQLKSLDLKRNMRETVGTKQRVREQNNECFSHEPSMKDSLAAVCNATQAAARIHQVFRVQSFQRKQQKEYDGDKFGISDERALSLISVNAKSHKSGLRNEPVHVAATRIQNKFRSWKGRKDFLIIRQRIVKIQAHVRGHQVRKNYGKIIWSVGIVEKVILRWRRKGSGLRGFKSEAISEGTMVQEGRKQTEKRLEKALARVKSMAQYPDARDQYHRLLNVVTEIQENQVKQDRNFINSEETRQFNNLVDLEALLDEDTFMPTAT*