AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023862 MtrunA17_Chr8g0382861 98.324 358 6 0 1 358 1 358 0.0 736
MsaG023862 MtrunA17_Chr3g0124601 68.785 362 105 4 4 358 3 363 0.0 510
MsaG023862 MtrunA17_Chr3g0115371 45.821 347 181 3 15 354 1 347 3.54e-102 305
MsaG023862 MtrunA17_Chr5g0424861 47.839 347 173 5 15 353 1 347 1.47e-98 296
MsaG023862 MtrunA17_Chr8g0353501 52.234 291 125 4 75 356 181 466 3.66e-94 288
MsaG023862 MtrunA17_Chr7g0256211 51.546 291 127 4 75 356 167 452 4.14e-93 285
MsaG023862 MtrunA17_Chr2g0321431 50.526 285 129 4 75 356 194 469 2.03e-90 280
MsaG023862 MtrunA17_Chr7g0258321 45.425 306 159 3 59 356 158 463 9.60e-88 272
MsaG023862 MtrunA17_Chr5g0422771 48.797 291 137 3 74 356 178 464 1.50e-86 269
MsaG023862 MtrunA17_Chr1g0174701 41.333 375 185 8 3 356 146 506 2.26e-86 270
MsaG023862 MtrunA17_Chr4g0026401 47.735 287 139 3 71 356 185 461 8.03e-85 265
MsaG023862 MtrunA17_Chr1g0210151 44.828 290 151 4 74 356 53 340 5.44e-81 250
MsaG023862 MtrunA17_Chr5g0424851 50.450 222 109 1 133 353 1 222 4.65e-67 218
MsaG023862 MtrunA17_Chr5g0424851 47.573 206 108 0 141 346 223 428 3.54e-59 197
MsaG023862 MtrunA17_Chr3g0093451 45.342 161 80 4 78 230 49 209 6.73e-40 140
MsaG023862 MtrunA17_Chr1g0196951 33.796 216 117 6 129 338 34 229 7.92e-30 114
MsaG023862 MtrunA17_Chr8g0384821 25.806 248 149 5 133 345 2 249 1.26e-13 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023862 AT5G65210.5 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G65210.7 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G65210.3 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G65210.1 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G65210.2 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G65210.4 60.383 366 132 5 4 357 3 367 2.82e-156 443
MsaG023862 AT5G10030.5 61.264 364 128 5 4 357 3 363 9.81e-156 442
MsaG023862 AT5G10030.3 61.264 364 128 5 4 357 3 363 9.81e-156 442
MsaG023862 AT5G10030.1 61.264 364 128 5 4 357 3 363 9.81e-156 442
MsaG023862 AT5G10030.4 61.264 364 128 5 4 357 3 363 9.81e-156 442
MsaG023862 AT5G10030.2 61.264 364 128 5 4 357 3 363 9.81e-156 442
MsaG023862 AT5G65210.6 65.646 294 98 2 67 357 4 297 9.37e-139 396
MsaG023862 AT1G77920.1 49.867 375 161 5 1 357 2 367 9.47e-124 361
MsaG023862 AT1G22070.1 51.093 366 157 5 13 356 17 382 5.38e-122 357
MsaG023862 AT5G06950.2 52.759 290 124 4 75 356 46 330 7.25e-93 281
MsaG023862 AT5G06950.4 52.759 290 124 4 75 356 46 330 7.25e-93 281
MsaG023862 AT5G06950.1 52.759 290 124 4 75 356 46 330 7.25e-93 281
MsaG023862 AT5G06950.5 52.759 290 124 4 75 356 46 330 7.25e-93 281
MsaG023862 AT5G06950.3 52.759 290 124 4 75 356 46 330 7.25e-93 281
MsaG023862 AT1G08320.2 50.704 284 130 4 75 356 54 329 7.96e-93 281
MsaG023862 AT1G08320.5 50.704 284 130 4 75 356 54 329 7.96e-93 281
MsaG023862 AT1G08320.1 50.704 284 130 4 75 356 178 453 2.40e-91 282
MsaG023862 AT1G08320.4 50.704 284 130 4 75 356 178 453 2.40e-91 282
MsaG023862 AT1G08320.3 50.704 284 130 4 75 356 178 453 2.40e-91 282
MsaG023862 AT3G12250.5 51.186 295 131 4 70 356 14 303 6.37e-91 275
MsaG023862 AT3G12250.6 51.186 295 131 4 70 356 41 330 9.47e-91 275
MsaG023862 AT3G12250.2 51.186 295 131 4 70 356 41 330 9.47e-91 275
MsaG023862 AT3G12250.7 51.186 295 131 4 70 356 41 330 9.47e-91 275
MsaG023862 AT3G12250.1 51.186 295 131 4 70 356 41 330 9.47e-91 275
MsaG023862 AT3G12250.3 51.186 295 131 4 70 356 35 324 1.53e-90 275
MsaG023862 AT3G12250.4 48.589 319 143 5 54 356 42 355 3.98e-90 275
MsaG023862 AT5G06960.3 50.865 289 131 3 75 356 26 310 2.33e-89 271
MsaG023862 AT5G06960.2 50.865 289 131 3 75 356 46 330 2.40e-89 272
MsaG023862 AT5G06960.1 50.865 289 131 3 75 356 46 330 2.40e-89 272
MsaG023862 AT5G06839.3 43.567 342 167 5 41 356 117 458 1.95e-85 266
MsaG023862 AT5G06839.1 45.779 308 151 4 65 356 108 415 2.85e-85 264
MsaG023862 AT5G06839.2 44.660 309 154 3 65 356 108 416 1.71e-83 259
MsaG023862 AT1G68640.1 43.182 308 166 3 56 356 147 452 7.96e-75 238
MsaG023862 AT1G58330.1 26.906 223 146 6 139 346 3 223 2.77e-14 72.0

Find 72 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCAGTGATATACAAAT+TGG 0.178312 4:+93169095 MsaT023862.1:CDS
TGATTATTTAACTTCATTTC+AGG 0.206692 4:-93167018 None:intergenic
GGATTGGATTCTAATAATTT+AGG 0.214016 4:+93168109 MsaT023862.1:CDS
TAAGAGTCGTTTGAAGAAAA+AGG 0.214551 4:+93167814 MsaT023862.1:CDS
TCTGTCAATGGTTCAATTAA+AGG 0.231131 4:-93169388 None:intergenic
CCATACACCAATTTACTATA+TGG 0.242264 4:+93166953 MsaT023862.1:CDS
TGCTCGATGGCTGCTTGCTT+TGG 0.257053 4:+93169910 MsaT023862.1:CDS
GTTCTTCCCATATAGTAAAT+TGG 0.257991 4:-93166960 None:intergenic
TAGTAACTGTGTTAGCTTCT+TGG 0.278886 4:-93167202 None:intergenic
ATTCCAACATTTGTCTGTTT+AGG 0.286691 4:-93169052 None:intergenic
TAGTCAGAGGATGCTTCTTT+TGG 0.297753 4:+93167149 MsaT023862.1:intron
CAAATTGGGGAACTTGTAAA+TGG 0.313309 4:+93169110 MsaT023862.1:CDS
TTTGTTTCCTCAAGAAATAT+TGG 0.341373 4:+93166919 MsaT023862.1:CDS
TTTGCTGGCTCTGTAAATTC+AGG 0.350309 4:+93168133 MsaT023862.1:CDS
GCATGACTTCTCAAGATTAT+AGG 0.355362 4:-93169428 None:intergenic
AAATTGCAGGGATTGTATAT+TGG 0.363352 4:+93168082 MsaT023862.1:intron
AGCTAACACAGTTACTAATA+AGG 0.363384 4:+93167208 MsaT023862.1:CDS
GTGTCGTTTAAAGCTGTTAC+AGG 0.364560 4:+93167935 MsaT023862.1:CDS
GCTCGATGGCTGCTTGCTTT+GGG 0.374474 4:+93169911 MsaT023862.1:CDS
CCATATAGTAAATTGGTGTA+TGG 0.382687 4:-93166953 None:intergenic
TGAAATGAAGTTAAATAATC+AGG 0.391737 4:+93167020 MsaT023862.1:CDS
ATTGTATATTGGTGGTGGAT+TGG 0.406379 4:+93168093 MsaT023862.1:CDS
AATAGAGAGGCTGGAAGCTC+TGG 0.410741 4:+93169567 MsaT023862.1:CDS
AGGTGTTACCTTTAGAAGTT+CGG 0.411150 4:-93169267 None:intergenic
ATATCAGTGATATACAAATT+GGG 0.413722 4:+93169096 MsaT023862.1:CDS
CTGTCAATGGTTCAATTAAA+GGG 0.420015 4:-93169387 None:intergenic
TCGACCCTCCGAACTTCTAA+AGG 0.428847 4:+93169259 MsaT023862.1:CDS
AACCGTTGTTCCTCTGTCAA+TGG 0.441867 4:-93169400 None:intergenic
TAAGGGATTTGAGCAAACTT+TGG 0.444298 4:+93169951 MsaT023862.1:CDS
GTGAATACTTTCAACGCCTA+AGG 0.464354 4:+93169933 MsaT023862.1:CDS
CATACACCAATTTACTATAT+GGG 0.478931 4:+93166954 MsaT023862.1:CDS
AGATGCCCTTTCACAAGGTA+TGG 0.480625 4:+93169462 MsaT023862.1:CDS
AGAGCTAGATCATACAAAAC+AGG 0.481991 4:+93167965 MsaT023862.1:CDS
GGATATTGCAATAGAGAGGC+TGG 0.484290 4:+93169558 MsaT023862.1:CDS
AAAGGGCATCTTCTGCTTGT+CGG 0.484492 4:-93169450 None:intergenic
TAACTGTGTTAGCTTCTTGG+TGG 0.484546 4:-93167199 None:intergenic
TCTGGCTAGCTTTGTGAACC+AGG 0.487895 4:+93169585 MsaT023862.1:CDS
CAGGGATTGTATATTGGTGG+TGG 0.489623 4:+93168088 MsaT023862.1:intron
CTTGTTGACACAGTTGCAGC+AGG 0.514923 4:+93169502 MsaT023862.1:CDS
CATATAGTAAATTGGTGTAT+GGG 0.519743 4:-93166952 None:intergenic
AAAGTTTGCTCAAATCCCTT+AGG 0.521782 4:-93169949 None:intergenic
AGACCTAAACAGACAAATGT+TGG 0.522384 4:+93169049 MsaT023862.1:CDS
TGCAGGTTCACGAGGACGAT+TGG 0.528063 4:-93169975 None:intergenic
TGAATACTTTCAACGCCTAA+GGG 0.549102 4:+93169934 MsaT023862.1:CDS
ATGTGGAAAACAACAGCTGA+AGG 0.550259 4:+93169212 MsaT023862.1:CDS
ACAAATTGAGAAGATGATGA+AGG 0.553463 4:-93166901 None:intergenic
AGACAAATGTTGGAATTGAA+AGG 0.555362 4:+93169059 MsaT023862.1:CDS
ACAGGCTGATCATCTTCGAC+AGG 0.558370 4:+93169844 MsaT023862.1:intron
GAAGAACATTTCAAGAGTAA+TGG 0.562707 4:+93166976 MsaT023862.1:CDS
GCAGCAGGACAACTCGTTGA+AGG 0.566836 4:+93169517 MsaT023862.1:CDS
CTCTTGCATGAAGACTATGC+AGG 0.569640 4:-93169992 None:intergenic
AGGAACTTACATTCCACAGA+TGG 0.575188 4:+93169537 MsaT023862.1:CDS
CCGTACGTCAAACTGCTCGA+TGG 0.578154 4:+93169897 MsaT023862.1:CDS
TATCAGTGATATACAAATTG+GGG 0.581840 4:+93169097 MsaT023862.1:CDS
ATATACACCAATATTTCTTG+AGG 0.587022 4:-93166926 None:intergenic
TTGCAGGGATTGTATATTGG+TGG 0.592067 4:+93168085 MsaT023862.1:intron
TAAGATGGAGTATGAACACT+GGG 0.600728 4:+93169022 MsaT023862.1:CDS
TGTTACCTTTAGAAGTTCGG+AGG 0.601165 4:-93169264 None:intergenic
TTAAGATGGAGTATGAACAC+TGG 0.603260 4:+93169021 MsaT023862.1:CDS
AACCATTGACAGAGGAACAA+CGG 0.614266 4:+93169398 MsaT023862.1:CDS
GCAGAAGATGCCCTTTCACA+AGG 0.620831 4:+93169457 MsaT023862.1:CDS
TTTAATTGAACCATTGACAG+AGG 0.625048 4:+93169390 MsaT023862.1:CDS
TCTATGTTATCACTGGCATG+TGG 0.630097 4:+93169195 MsaT023862.1:CDS
CCATCGAGCAGTTTGACGTA+CGG 0.630335 4:-93169897 None:intergenic
TCTATTGCAATATCCATCTG+TGG 0.636700 4:-93169550 None:intergenic
GTTACCTTTAGAAGTTCGGA+GGG 0.640489 4:-93169263 None:intergenic
GAAGACTATGCAGGTTCACG+AGG 0.645466 4:-93169983 None:intergenic
AGATGGATATTGCAATAGAG+AGG 0.647680 4:+93169554 MsaT023862.1:CDS
TGTGGAAAACAACAGCTGAA+GGG 0.647786 4:+93169213 MsaT023862.1:CDS
GTTTGAAATAGTGATTCATG+AGG 0.660995 4:-93169133 None:intergenic
ACGTCTCGCACAAAATCGAG+AGG 0.680873 4:+93167784 MsaT023862.1:CDS
GATGGAGTATGAACACTGGG+TGG 0.682481 4:+93169025 MsaT023862.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 93166895 93170000 93166895 ID=MsaG023862
Chr4 mRNA 93166895 93170000 93166895 ID=MsaT023862.1;Parent=MsaG023862
Chr4 exon 93166895 93167041 93166895 ID=MsaT023862.1.exon1;Parent=MsaT023862.1
Chr4 CDS 93166895 93167041 93166895 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93167152 93167229 93167152 ID=MsaT023862.1.exon2;Parent=MsaT023862.1
Chr4 CDS 93167152 93167229 93167152 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93167776 93167835 93167776 ID=MsaT023862.1.exon3;Parent=MsaT023862.1
Chr4 CDS 93167776 93167835 93167776 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93167912 93167986 93167912 ID=MsaT023862.1.exon4;Parent=MsaT023862.1
Chr4 CDS 93167912 93167986 93167912 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93168091 93168154 93168091 ID=MsaT023862.1.exon5;Parent=MsaT023862.1
Chr4 CDS 93168091 93168154 93168091 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93169009 93169280 93169009 ID=MsaT023862.1.exon6;Parent=MsaT023862.1
Chr4 CDS 93169009 93169280 93169009 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93169379 93169606 93169379 ID=MsaT023862.1.exon7;Parent=MsaT023862.1
Chr4 CDS 93169379 93169606 93169379 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Chr4 exon 93169848 93170000 93169848 ID=MsaT023862.1.exon8;Parent=MsaT023862.1
Chr4 CDS 93169848 93170000 93169848 ID=cds.MsaT023862.1;Parent=MsaT023862.1
Gene Sequence

>MsaT023862.1

ATGGATCCTTCATCATCTTCTCAATTTGTTTCCTCAAGAAATATTGGTGTATATGATCCCATACACCAATTTACTATATGGGAAGAACATTTCAAGAGTAATGGTGATACAAGTGTATATGAACCTGAAATGAAGTTAAATAATCAGTCAGAGGATGCTTCTTTTGGAATATTGGGAACATCTATCAAGTATGACCACCAAGAAGCTAACACAGTTACTAATAAGATGCAAAGACGTCTCGCACAAAATCGAGAGGCTGCTCGTAAGAGTCGTTTGAAGAAAAAGGCTCATATACAACAGTTAGAATCGTGTCGTTTAAAGCTGTTACAGGTAGAGCAAGAGCTAGATCATACAAAACAGGGATTGTATATTGGTGGTGGATTGGATTCTAATAATTTAGGTTTTGCTGGCTCTGTAAATTCAGAAATAACAACTTTTAAGATGGAGTATGAACACTGGGTGGAAGACCTAAACAGACAAATGTTGGAATTGAAAGGTGCTTTAAGTGCTCATATCAGTGATATACAAATTGGGGAACTTGTAAATGGCCTCATGAATCACTATTTCAAACTATTTTGCATGAAATCTGATGCTGCAAAAGTAGATGTTTTCTATGTTATCACTGGCATGTGGAAAACAACAGCTGAAGGGTTTTTCTTGTGGATTGGAGGTTTTCGACCCTCCGAACTTCTAAAGGTTCTGGTCCCTTTAATTGAACCATTGACAGAGGAACAACGGTTTGATGCCTATAATCTTGAGAAGTCATGCCGACAAGCAGAAGATGCCCTTTCACAAGGTATGGAAAAACTACAGGGAATGCTTGTTGACACAGTTGCAGCAGGACAACTCGTTGAAGGAACTTACATTCCACAGATGGATATTGCAATAGAGAGGCTGGAAGCTCTGGCTAGCTTTGTGAACCAGGCTGATCATCTTCGACAGGAAACGTTGCAACAGATGTCTCGAATACTTACCGTACGTCAAACTGCTCGATGGCTGCTTGCTTTGGGTGAATACTTTCAACGCCTAAGGGATTTGAGCAAACTTTGGACCAATCGTCCTCGTGAACCTGCATAG
Protein sequence

>MsaT023862.1

MDPSSSSQFVSSRNIGVYDPIHQFTIWEEHFKSNGDTSVYEPEMKLNNQSEDASFGILGTSIKYDHQEANTVTNKMQRRLAQNREAARKSRLKKKAHIQQLESCRLKLLQVEQELDHTKQGLYIGGGLDSNNLGFAGSVNSEITTFKMEYEHWVEDLNRQMLELKGALSAHISDIQIGELVNGLMNHYFKLFCMKSDAAKVDVFYVITGMWKTTAEGFFLWIGGFRPSELLKVLVPLIEPLTEEQRFDAYNLEKSCRQAEDALSQGMEKLQGMLVDTVAAGQLVEGTYIPQMDIAIERLEALASFVNQADHLRQETLQQMSRILTVRQTARWLLALGEYFQRLRDLSKLWTNRPREPA*