AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023889

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023889 MtrunA17_Chr8g0383251 95.109 184 8 1 1 183 1 184 5.56e-130 362
MsaG023889 MtrunA17_Chr3g0129321 54.545 198 69 8 1 182 1 193 1.22e-56 177
MsaG023889 MtrunA17_Chr1g0150801 48.252 143 58 3 28 163 25 158 9.13e-36 122
MsaG023889 MtrunA17_Chr2g0294231 58.427 89 36 1 90 178 186 273 1.63e-30 114
MsaG023889 MtrunA17_Chr7g0218081 42.975 121 56 1 65 172 355 475 7.94e-29 111
MsaG023889 MtrunA17_Chr7g0218081 56.667 60 25 1 107 166 228 286 2.60e-14 70.1
MsaG023889 MtrunA17_Chr7g0270191 46.957 115 54 2 65 174 100 212 3.08e-28 106
MsaG023889 MtrunA17_Chr4g0051881 58.108 74 31 0 93 166 164 237 1.24e-27 105
MsaG023889 MtrunA17_Chr4g0004911 39.850 133 78 1 35 167 266 396 1.56e-27 107
MsaG023889 MtrunA17_Chr4g0004911 38.793 116 61 3 57 166 132 243 3.64e-16 75.5
MsaG023889 MtrunA17_Chr3g0101131 38.889 144 75 2 42 172 337 480 1.62e-27 108
MsaG023889 MtrunA17_Chr3g0101131 49.206 63 31 1 107 169 243 304 6.94e-11 60.1
MsaG023889 MtrunA17_Chr1g0194481 51.087 92 42 1 81 172 135 223 2.48e-27 105
MsaG023889 MtrunA17_Chr5g0443131 59.211 76 31 0 93 168 143 218 8.94e-27 103
MsaG023889 MtrunA17_Chr4g0042831 46.078 102 42 1 78 166 236 337 9.69e-27 104
MsaG023889 MtrunA17_Chr4g0042831 49.206 63 32 0 107 169 145 207 2.08e-12 64.3
MsaG023889 MtrunA17_Chr5g0445311 48.889 90 46 0 93 182 350 439 1.34e-26 105
MsaG023889 MtrunA17_Chr5g0445311 50.000 60 29 1 107 166 200 258 7.26e-11 60.1
MsaG023889 MtrunA17_Chr3g0079691 52.439 82 39 0 93 174 481 562 1.47e-26 105
MsaG023889 MtrunA17_Chr3g0079691 49.275 69 34 1 107 175 280 347 6.80e-13 66.2
MsaG023889 MtrunA17_Chr5g0418191 58.108 74 31 0 93 166 306 379 1.53e-26 104
MsaG023889 MtrunA17_Chr5g0418191 53.333 60 27 1 107 166 151 209 9.64e-13 65.5
MsaG023889 MtrunA17_Chr7g0243891 56.757 74 32 0 93 166 360 433 1.81e-26 105
MsaG023889 MtrunA17_Chr7g0243891 52.308 65 30 1 102 166 211 274 5.22e-15 72.0
MsaG023889 MtrunA17_Chr7g0249311 51.807 83 40 0 93 175 378 460 2.14e-26 105
MsaG023889 MtrunA17_Chr7g0249311 52.381 63 29 1 107 169 179 240 5.41e-13 66.2
MsaG023889 MtrunA17_Chr7g0224801 56.757 74 32 0 96 169 82 155 2.67e-26 98.6
MsaG023889 MtrunA17_Chr7g0249301 51.807 83 39 1 84 166 270 351 2.87e-26 103
MsaG023889 MtrunA17_Chr7g0249301 50.000 64 31 1 106 169 61 123 2.71e-12 63.9
MsaG023889 MtrunA17_Chr8g0335811 43.697 119 59 2 51 163 94 210 6.86e-26 99.0
MsaG023889 MtrunA17_Chr5g0418781 47.664 107 47 2 57 163 83 180 5.63e-25 97.8
MsaG023889 MtrunA17_Chr7g0248721 47.059 102 48 2 83 179 117 217 1.65e-24 95.1
MsaG023889 MtrunA17_Chr3g0106491 51.250 80 39 0 87 166 345 424 1.83e-24 99.4
MsaG023889 MtrunA17_Chr3g0106491 41.000 100 54 2 67 166 162 256 1.68e-14 70.5
MsaG023889 MtrunA17_Chr4g0068541 48.810 84 43 0 94 177 195 278 3.25e-24 97.8
MsaG023889 MtrunA17_Chr5g0432161 54.054 74 34 0 93 166 407 480 9.03e-24 97.4
MsaG023889 MtrunA17_Chr5g0432161 53.333 60 27 1 107 166 244 302 2.11e-13 67.4
MsaG023889 MtrunA17_Chr3g0129361 54.930 71 32 0 93 163 130 200 2.00e-23 92.4
MsaG023889 MtrunA17_Chr7g0263531 49.333 75 38 0 93 167 347 421 2.42e-22 92.8
MsaG023889 MtrunA17_Chr7g0263531 54.237 59 26 1 108 166 190 247 5.31e-12 63.2
MsaG023889 MtrunA17_Chr5g0447701 54.795 73 33 0 94 166 239 311 4.97e-22 91.7
MsaG023889 MtrunA17_Chr1g0189391 53.947 76 34 1 89 163 79 154 5.11e-22 90.9
MsaG023889 MtrunA17_Chr8g0386241 51.389 72 35 0 94 165 90 161 6.79e-22 87.4
MsaG023889 MtrunA17_Chr8g0383351 54.167 72 32 1 93 163 145 216 1.34e-21 88.2
MsaG023889 MtrunA17_Chr4g0074001 48.750 80 41 0 88 167 340 419 4.31e-21 89.7
MsaG023889 MtrunA17_Chr6g0485431 45.122 82 44 1 89 169 136 217 4.85e-21 88.6
MsaG023889 MtrunA17_Chr1g0188941 47.297 74 39 0 93 166 37 110 7.25e-21 83.2
MsaG023889 MtrunA17_Chr7g0270831 47.297 74 38 1 94 166 203 276 1.35e-20 87.8
MsaG023889 MtrunA17_Chr4g0075201 45.946 74 39 1 94 166 254 327 6.88e-20 86.3
MsaG023889 MtrunA17_Chr4g0051181 48.000 75 38 1 94 167 230 304 3.00e-19 84.3
MsaG023889 MtrunA17_Chr7g0238921 55.932 59 26 0 105 163 122 180 3.76e-19 82.8
MsaG023889 MtrunA17_Chr3g0099641 44.156 77 42 1 94 169 238 314 5.70e-19 83.6
MsaG023889 MtrunA17_Chr2g0302391 47.297 74 38 1 94 166 198 271 2.06e-18 81.6
MsaG023889 MtrunA17_Chr2g0322391 44.595 74 40 1 94 166 336 409 2.18e-18 82.0
MsaG023889 MtrunA17_Chr4g0053281 45.783 83 41 2 84 165 246 325 2.82e-18 80.9
MsaG023889 MtrunA17_Chr1g0147881 43.902 82 45 1 85 165 240 321 7.44e-18 79.7
MsaG023889 MtrunA17_Chr4g0060271 44.000 75 41 1 94 167 242 316 1.28e-17 79.7
MsaG023889 MtrunA17_Chr2g0288871 45.946 74 39 1 94 166 292 365 2.15e-17 79.0
MsaG023889 MtrunA17_Chr7g0216891 42.857 77 43 1 94 169 206 282 2.65e-17 79.0
MsaG023889 MtrunA17_Chr4g0025051 45.455 77 41 1 94 169 322 398 2.74e-17 79.0
MsaG023889 MtrunA17_Chr5g0401821 47.945 73 37 1 94 165 186 258 2.91e-17 77.0
MsaG023889 MtrunA17_Chr2g0333851 47.368 76 37 2 94 166 154 229 4.76e-17 77.0
MsaG023889 MtrunA17_Chr5g0447711 38.824 85 52 0 82 166 205 289 1.51e-16 75.9
MsaG023889 MtrunA17_Chr3g0136961 50.847 59 28 1 106 163 137 195 2.13e-16 75.1
MsaG023889 MtrunA17_Chr1g0184691 42.500 80 45 1 85 163 271 350 6.35e-16 74.3
MsaG023889 MtrunA17_Chr4g0001361 50.794 63 30 1 105 166 160 222 1.04e-15 73.6
MsaG023889 MtrunA17_Chr8g0379461 44.286 70 38 1 108 176 77 146 2.34e-15 71.6
MsaG023889 MtrunA17_Chr4g0025061 43.243 74 41 1 94 166 347 420 4.11e-15 72.4
MsaG023889 MtrunA17_Chr2g0293481 37.755 98 59 2 71 166 230 327 6.81e-15 71.6
MsaG023889 MtrunA17_Chr4g0071521 50.820 61 29 1 104 163 242 302 8.74e-15 70.9
MsaG023889 MtrunA17_Chr2g0319961 43.662 71 39 1 98 167 71 141 1.12e-14 70.1
MsaG023889 MtrunA17_Chr7g0270051 50.000 60 29 1 108 166 54 113 1.69e-14 69.7
MsaG023889 MtrunA17_Chr8g0348311 46.875 64 33 1 104 166 152 215 3.97e-14 69.3
MsaG023889 MtrunA17_Chr7g0228941 33.636 110 67 3 56 164 6 110 4.42e-14 65.5
MsaG023889 MtrunA17_Chr1g0150281 45.614 57 30 1 108 163 141 197 1.28e-13 67.4
MsaG023889 MtrunA17_Chr7g0248691 47.619 63 32 1 105 166 152 214 1.34e-13 67.8
MsaG023889 MtrunA17_Chr1g0202461 39.759 83 47 2 85 166 241 321 1.66e-13 67.8
MsaG023889 MtrunA17_Chr2g0289181 43.939 66 36 1 102 166 9 74 1.01e-12 64.3
MsaG023889 MtrunA17_Chr5g0432071 50.794 63 30 1 107 169 104 165 1.16e-12 63.5
MsaG023889 MtrunA17_Chr2g0284321 39.080 87 49 2 81 166 12 95 1.63e-12 63.5
MsaG023889 MtrunA17_Chr1g0150271 41.270 63 36 1 105 166 99 161 2.22e-12 63.5
MsaG023889 MtrunA17_Chr3g0139021 44.262 61 33 1 104 163 311 371 2.69e-12 64.3
MsaG023889 MtrunA17_Chr8g0387501 46.667 60 31 1 108 166 130 189 2.87e-12 63.5
MsaG023889 MtrunA17_Chr4g0024391 37.662 77 47 1 94 169 216 292 9.98e-12 62.8
MsaG023889 MtrunA17_Chr5g0402931 45.000 60 31 1 104 163 120 177 1.78e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023889 AT5G64810.1 76.000 75 18 0 90 164 93 167 2.91e-38 130
MsaG023889 AT5G26170.1 75.714 70 17 0 94 163 100 169 2.02e-35 122
MsaG023889 AT5G49520.1 60.000 75 30 0 94 168 208 282 1.19e-28 110
MsaG023889 AT1G29860.1 61.333 75 29 0 94 168 123 197 4.15e-28 106
MsaG023889 AT4G18170.1 56.962 79 34 0 88 166 153 231 1.10e-27 106
MsaG023889 AT5G46350.1 49.451 91 46 0 93 183 169 259 2.94e-27 105
MsaG023889 AT2G47260.1 51.613 93 43 1 77 169 146 236 3.87e-27 105
MsaG023889 AT3G62340.1 61.333 75 29 0 93 167 104 178 4.90e-27 103
MsaG023889 AT3G01970.1 55.556 72 32 0 94 165 52 123 9.15e-27 99.4
MsaG023889 AT2G46130.1 55.128 78 35 0 86 163 9 86 1.50e-26 97.8
MsaG023889 AT2G38470.1 56.757 74 32 0 93 166 348 421 3.80e-26 104
MsaG023889 AT2G38470.1 51.667 60 28 1 107 166 184 242 2.47e-12 64.7
MsaG023889 AT5G41570.1 52.500 80 38 0 84 163 75 154 1.50e-25 97.4
MsaG023889 AT5G13080.1 56.944 72 31 0 94 165 54 125 1.57e-25 96.3
MsaG023889 AT1G69310.4 57.534 73 31 0 93 165 133 205 1.91e-25 99.8
MsaG023889 AT1G69310.2 57.534 73 31 0 93 165 133 205 1.91e-25 99.8
MsaG023889 AT1G69310.1 57.534 73 31 0 93 165 133 205 1.91e-25 99.8
MsaG023889 AT2G44745.1 44.444 126 66 3 42 166 81 203 2.72e-25 97.8
MsaG023889 AT3G01080.2 55.405 74 33 0 93 166 330 403 3.19e-25 101
MsaG023889 AT3G01080.2 53.333 60 27 1 107 166 205 263 3.45e-13 67.4
MsaG023889 AT5G56270.1 55.405 74 33 0 93 166 473 546 3.30e-25 102
MsaG023889 AT5G56270.1 53.333 60 27 1 107 166 273 331 7.72e-13 66.2
MsaG023889 AT3G01080.1 55.405 74 33 0 93 166 292 365 3.45e-25 100
MsaG023889 AT3G01080.1 53.333 60 27 1 107 166 167 225 4.33e-13 67.0
MsaG023889 AT5G07100.2 55.405 74 33 0 93 166 127 200 4.12e-25 97.1
MsaG023889 AT5G07100.2 41.772 79 44 1 107 183 24 102 4.36e-12 62.8
MsaG023889 AT5G07100.4 55.405 74 33 0 93 166 127 200 4.12e-25 97.1
MsaG023889 AT5G07100.4 41.772 79 44 1 107 183 24 102 4.36e-12 62.8
MsaG023889 AT5G07100.5 55.405 74 33 0 93 166 127 200 4.12e-25 97.1
MsaG023889 AT5G07100.5 41.772 79 44 1 107 183 24 102 4.36e-12 62.8
MsaG023889 AT5G07100.3 55.405 74 33 0 93 166 127 200 4.12e-25 97.1
MsaG023889 AT5G07100.3 41.772 79 44 1 107 183 24 102 4.36e-12 62.8
MsaG023889 AT1G13960.2 54.054 74 34 0 93 166 242 315 4.42e-25 100
MsaG023889 AT1G13960.2 50.667 75 33 2 95 166 61 134 1.00e-15 74.3
MsaG023889 AT1G64000.1 53.659 82 38 0 82 163 89 170 4.98e-25 96.3
MsaG023889 AT1G13960.1 54.054 74 34 0 93 166 395 468 6.06e-25 100
MsaG023889 AT1G13960.1 50.667 75 33 2 95 166 214 287 6.63e-16 75.1
MsaG023889 AT2G21900.1 47.917 96 46 2 85 176 83 178 6.25e-25 96.3
MsaG023889 AT5G07100.1 52.439 82 39 0 85 166 212 293 6.53e-25 98.6
MsaG023889 AT5G07100.1 41.772 79 44 1 107 183 117 195 3.57e-12 63.9
MsaG023889 AT4G26640.5 51.250 80 39 0 93 172 191 270 8.09e-25 99.4
MsaG023889 AT4G26640.5 50.000 62 30 1 105 166 33 93 2.55e-11 61.6
MsaG023889 AT4G26640.6 51.250 80 39 0 93 172 191 270 8.09e-25 99.4
MsaG023889 AT4G26640.6 50.000 62 30 1 105 166 33 93 2.55e-11 61.6
MsaG023889 AT4G26640.4 51.250 80 39 0 93 172 191 270 8.09e-25 99.4
MsaG023889 AT4G26640.4 50.000 62 30 1 105 166 33 93 2.55e-11 61.6
MsaG023889 AT4G26640.3 51.250 80 39 0 93 172 191 270 8.09e-25 99.4
MsaG023889 AT4G26640.3 50.000 62 30 1 105 166 33 93 2.55e-11 61.6
MsaG023889 AT4G26640.7 51.250 80 39 0 93 172 327 406 1.11e-24 100
MsaG023889 AT4G26640.7 50.000 62 30 1 105 166 169 229 1.36e-11 62.8
MsaG023889 AT4G26640.1 51.250 80 39 0 93 172 295 374 1.14e-24 100
MsaG023889 AT4G26640.1 50.000 62 30 1 105 166 137 197 1.43e-11 62.4
MsaG023889 AT4G26640.2 51.250 80 39 0 93 172 367 446 1.29e-24 100
MsaG023889 AT4G26640.2 50.000 62 30 1 105 166 209 269 1.34e-11 62.8
MsaG023889 AT4G39410.1 52.326 86 40 1 78 163 195 279 7.33e-24 95.9
MsaG023889 AT2G03340.1 52.703 74 35 0 93 166 401 474 8.32e-24 97.8
MsaG023889 AT2G03340.1 56.667 60 25 1 107 166 250 308 4.87e-15 72.8
MsaG023889 AT2G04880.2 42.857 112 59 2 58 166 233 342 1.31e-23 97.1
MsaG023889 AT2G04880.2 43.750 64 35 1 104 167 108 170 5.00e-11 60.8
MsaG023889 AT2G04880.1 42.857 112 59 2 58 166 257 366 1.50e-23 97.1
MsaG023889 AT2G04880.1 43.750 64 35 1 104 167 108 170 5.48e-11 60.8
MsaG023889 AT4G26440.1 48.780 82 42 0 93 174 358 439 1.89e-23 97.1
MsaG023889 AT4G26440.1 51.667 60 28 1 107 166 178 236 3.80e-11 61.2
MsaG023889 AT2G46130.2 52.564 78 33 1 86 163 9 82 4.64e-23 88.6
MsaG023889 AT2G30250.1 45.556 90 43 1 77 166 304 387 2.66e-22 92.8
MsaG023889 AT2G30250.1 48.649 74 36 2 107 179 166 238 5.70e-11 60.8
MsaG023889 AT1G69310.3 53.425 73 30 1 93 165 133 201 1.02e-21 89.7
MsaG023889 AT4G04450.2 47.297 74 38 1 94 166 185 258 3.38e-20 87.0
MsaG023889 AT5G43290.1 62.712 59 22 0 105 163 112 170 4.25e-20 85.1
MsaG023889 AT1G55600.1 42.045 88 51 0 79 166 279 366 5.48e-20 86.7
MsaG023889 AT4G04450.1 47.297 74 38 1 94 166 279 352 1.00e-19 86.3
MsaG023889 AT1G68150.1 46.667 75 39 1 94 167 222 296 2.98e-19 84.3
MsaG023889 AT2G23320.1 47.674 86 41 2 81 165 217 299 4.37e-19 83.2
MsaG023889 AT4G30935.1 45.946 74 40 0 94 167 318 391 4.91e-19 84.0
MsaG023889 AT1G62300.1 47.297 74 38 1 94 166 299 372 4.94e-19 84.3
MsaG023889 AT5G15130.2 44.156 77 42 1 94 169 172 248 6.86e-19 83.6
MsaG023889 AT5G15130.1 44.156 77 42 1 94 169 214 290 7.89e-19 83.6
MsaG023889 AT4G22070.4 47.297 74 38 1 94 166 284 357 8.61e-19 83.6
MsaG023889 AT4G22070.3 47.297 74 38 1 94 166 284 357 8.61e-19 83.6
MsaG023889 AT4G22070.2 47.297 74 38 1 94 166 284 357 8.61e-19 83.6
MsaG023889 AT4G22070.1 47.297 74 38 1 94 166 284 357 8.61e-19 83.6
MsaG023889 AT4G01720.1 44.595 74 40 1 94 166 226 299 2.85e-18 82.0
MsaG023889 AT1G18860.1 44.595 74 40 1 94 166 226 299 1.81e-17 79.7
MsaG023889 AT4G24240.1 53.968 63 28 1 104 165 278 340 3.12e-17 78.2
MsaG023889 AT3G04670.1 46.739 92 39 3 73 163 237 319 7.39e-17 77.0
MsaG023889 AT1G29280.1 50.794 63 30 1 108 169 75 137 7.73e-17 76.3
MsaG023889 AT2G25000.2 47.692 65 33 1 106 169 102 166 1.07e-16 75.1
MsaG023889 AT2G25000.4 47.692 65 33 1 106 169 102 166 1.07e-16 75.1
MsaG023889 AT2G25000.3 47.692 65 33 1 106 169 124 188 1.51e-16 75.1
MsaG023889 AT1G80840.1 46.032 63 33 1 105 166 144 206 1.97e-16 75.5
MsaG023889 AT2G25000.1 47.692 65 33 1 106 169 145 209 2.68e-16 74.7
MsaG023889 AT5G28650.1 45.652 92 40 3 73 163 237 319 3.02e-16 75.5
MsaG023889 AT2G24570.1 37.500 104 63 2 62 163 197 300 3.55e-16 75.1
MsaG023889 AT2G34830.1 42.391 92 45 3 83 166 184 275 4.56e-16 75.5
MsaG023889 AT2G34830.2 42.391 92 45 3 83 166 193 284 4.97e-16 75.5
MsaG023889 AT4G31550.2 47.727 88 36 4 79 163 222 302 6.74e-16 74.3
MsaG023889 AT4G31550.1 41.250 80 46 1 85 163 224 303 1.10e-15 73.9
MsaG023889 AT1G30650.1 40.860 93 47 3 83 167 186 278 1.73e-15 73.9
MsaG023889 AT4G31800.2 43.939 66 36 1 106 170 110 175 3.63e-15 71.6
MsaG023889 AT3G58710.2 36.220 127 72 4 51 169 8 133 4.38e-15 71.6
MsaG023889 AT4G31800.1 43.939 66 36 1 106 170 175 240 6.51e-15 71.6
MsaG023889 AT2G37260.2 46.875 64 34 0 102 165 262 325 6.80e-15 71.6
MsaG023889 AT2G37260.2 41.237 97 48 3 78 166 46 141 3.91e-13 66.6
MsaG023889 AT2G37260.3 46.875 64 34 0 102 165 264 327 6.87e-15 71.6
MsaG023889 AT2G37260.3 41.237 97 48 3 78 166 48 143 3.90e-13 66.6
MsaG023889 AT2G37260.1 46.875 64 34 0 102 165 344 407 7.26e-15 72.0
MsaG023889 AT2G37260.1 41.237 97 48 3 78 166 128 223 4.32e-13 67.0
MsaG023889 AT2G30590.1 52.459 61 28 1 104 163 310 370 1.43e-14 70.9
MsaG023889 AT3G58710.1 45.205 73 39 1 98 169 62 134 1.52e-14 70.1
MsaG023889 AT4G01250.1 50.000 60 29 1 108 166 129 188 1.19e-13 67.8
MsaG023889 AT3G01080.3 53.333 60 27 1 107 166 205 263 1.65e-13 67.8
MsaG023889 AT4G23550.1 43.939 66 36 1 102 166 135 200 2.48e-13 67.0
MsaG023889 AT1G69810.2 37.079 89 48 3 81 167 125 207 6.20e-13 66.2
MsaG023889 AT1G69810.1 37.079 89 48 3 81 167 183 265 9.87e-13 65.9
MsaG023889 AT5G52830.1 47.619 63 32 1 105 166 163 225 1.99e-12 64.7
MsaG023889 AT5G45050.4 48.333 60 30 1 108 166 1042 1101 4.91e-12 63.9
MsaG023889 AT5G45050.1 48.333 60 30 1 108 166 1181 1240 5.15e-12 63.9
MsaG023889 AT5G45050.2 48.333 60 30 1 108 166 1153 1212 5.55e-12 63.9
MsaG023889 AT1G66560.1 37.079 89 44 3 97 182 93 172 2.91e-11 60.8

Find 40 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATCAGAGCTTGAGATTA+TGG 0.274000 4:-93431476 MsaT023889.1:CDS
CTTGTTCTAAAGGCTATCTT+TGG 0.312484 4:+93431496 None:intergenic
TGAGTAGAGTGAGCAAAATT+TGG 0.322485 4:+93432135 None:intergenic
TGCATCATTGAATTCTTGAT+TGG 0.365316 4:+93431978 None:intergenic
CAGAATGCACAAAAGAGATT+TGG 0.367718 4:+93430209 None:intergenic
AAATGGGATTAAGCGAAATA+AGG 0.381517 4:-93431530 MsaT023889.1:CDS
TTACATATTATTGTTCTTTG+AGG 0.400718 4:+93431954 None:intergenic
TTGCTTGATGATATTTGTAT+TGG 0.407278 4:-93432066 MsaT023889.1:CDS
TGGCTGCAGTGGGAGGTAAA+TGG 0.416069 4:+93430229 None:intergenic
CAAAAGAGATTTGGCTGCAG+TGG 0.429261 4:+93430218 None:intergenic
TGTTACCTTCTCTAATGATT+CGG 0.455426 4:+93432011 None:intergenic
ATAAACTCAGAAGAGGGATT+AGG 0.455505 4:+93432105 None:intergenic
TGTGAAGAAAAGGGTGGAAA+GGG 0.465962 4:-93430311 MsaT023889.1:CDS
GGATACAAGTGGAGGAAGTA+CGG 0.474552 4:-93431448 MsaT023889.1:CDS
AAGCTCTGATCTTGTTCTAA+AGG 0.476991 4:+93431486 None:intergenic
GAGCTTGAGATTATGGATGA+TGG 0.492012 4:-93431469 MsaT023889.1:CDS
TGGGATGTAATGTGAAGAAA+AGG 0.500798 4:-93430321 MsaT023889.1:CDS
GTGACCGAGATTCTTGATCA+AGG 0.501478 4:+93432040 None:intergenic
AAGCGAAATAAGGTAGAAGC+GGG 0.536791 4:-93431520 MsaT023889.1:CDS
ATGTGAAGAAAAGGGTGGAA+AGG 0.539725 4:-93430312 MsaT023889.1:CDS
CAAAATTTGGAGTAGCATGA+GGG 0.551934 4:+93432148 None:intergenic
GATACAAGTGGAGGAAGTAC+GGG 0.554483 4:-93431447 MsaT023889.1:CDS
TGAAGAACAGTCCTAACCTA+AGG 0.563814 4:-93431414 MsaT023889.1:intron
TATGTGATAACAAGTTATGA+AGG 0.564114 4:-93430271 MsaT023889.1:CDS
TACTACAAATGTTCAAGTGT+GGG 0.566302 4:-93430340 MsaT023889.1:CDS
GTCACCTTGATCAAGAATCT+CGG 0.568789 4:-93432044 MsaT023889.1:CDS
AAAAGAGATTTGGCTGCAGT+GGG 0.577682 4:+93430219 None:intergenic
GCAAAATTTGGAGTAGCATG+AGG 0.589246 4:+93432147 None:intergenic
CTACTACAAATGTTCAAGTG+TGG 0.597033 4:-93430341 MsaT023889.1:CDS
TTATGGATGATGGATACAAG+TGG 0.599156 4:-93431459 MsaT023889.1:CDS
GATAGTATAAACTCAGAAGA+GGG 0.603484 4:+93432099 None:intergenic
AGATAGTATAAACTCAGAAG+AGG 0.605793 4:+93432098 None:intergenic
AAGTACCGAATCATTAGAGA+AGG 0.606756 4:-93432016 MsaT023889.1:CDS
GGGAGGTAAATGGAATCTCA+TGG 0.608064 4:+93430239 None:intergenic
AGAGATTTGGCTGCAGTGGG+AGG 0.615268 4:+93430222 None:intergenic
TAAGCGAAATAAGGTAGAAG+CGG 0.616655 4:-93431521 MsaT023889.1:CDS
TGGATGATGGATACAAGTGG+AGG 0.653822 4:-93431456 MsaT023889.1:CDS
GGGATGTAATGTGAAGAAAA+GGG 0.671707 4:-93430320 MsaT023889.1:CDS
ATGTAATGTGAAGAAAAGGG+TGG 0.682851 4:-93430317 MsaT023889.1:CDS
AGCGAAATAAGGTAGAAGCG+GGG 0.727454 4:-93431519 MsaT023889.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 93430195 93432190 93430195 ID=MsaG023889
Chr4 mRNA 93430195 93432190 93430195 ID=MsaT023889.1;Parent=MsaG023889
Chr4 exon 93430195 93430366 93430195 ID=MsaT023889.1.exon3;Parent=MsaT023889.1
Chr4 CDS 93430195 93430366 93430195 ID=cds.MsaT023889.1;Parent=MsaT023889.1
Chr4 exon 93431415 93431561 93431415 ID=MsaT023889.1.exon2;Parent=MsaT023889.1
Chr4 CDS 93431415 93431561 93431415 ID=cds.MsaT023889.1;Parent=MsaT023889.1
Chr4 exon 93431958 93432190 93431958 ID=MsaT023889.1.exon1;Parent=MsaT023889.1
Chr4 CDS 93431958 93432190 93431958 ID=cds.MsaT023889.1;Parent=MsaT023889.1
Gene Sequence

>MsaT023889.1

ATGGACTACTATTTTGTAAACCCTCATGCTACTCCAAATTTTGCTCACTCTACTCATATGGTTCCTAATCCCTCTTCTGAGTTTATACTATCTGATTATCTTTTGCTTGATGATATTTGTATTGGTCACCTTGATCAAGAATCTCGGTCACAAAGTACCGAATCATTAGAGAAGGTAACATTCAATGATGCCAATCAAGAATTCAATGATGCAACCTCAAAGAACAATAATATAAAATACAAAAATGGGATTAAGCGAAATAAGGTAGAAGCGGGGCCAAAGATAGCCTTTAGAACAAGATCAGAGCTTGAGATTATGGATGATGGATACAAGTGGAGGAAGTACGGGAAGAAGTCTGTGAAGAACAGTCCTAACCTAAGGAACTACTACAAATGTTCAAGTGTGGGATGTAATGTGAAGAAAAGGGTGGAAAGGGATAGAGATGATTCGAGCTATGTGATAACAAGTTATGAAGGTGTGCATAACCATGAGATTCCATTTACCTCCCACTGCAGCCAAATCTCTTTTGTGCATTCTGATTCATATAATTAA
Protein sequence

>MsaT023889.1

MDYYFVNPHATPNFAHSTHMVPNPSSEFILSDYLLLDDICIGHLDQESRSQSTESLEKVTFNDANQEFNDATSKNNNIKYKNGIKRNKVEAGPKIAFRTRSELEIMDDGYKWRKYGKKSVKNSPNLRNYYKCSSVGCNVKKRVERDRDDSSYVITSYEGVHNHEIPFTSHCSQISFVHSDSYN*