AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023942


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023942 MtrunA17_Chr2g0293381 65.323 248 77 1 1 248 1 239 6.05e-109 316
MsaG023942 MtrunA17_Chr8g0384671 64.800 250 73 4 1 248 1 237 2.36e-105 307
MsaG023942 MtrunA17_Chr8g0383821 97.600 125 3 0 1 125 1 125 3.59e-86 252
MsaG023942 MtrunA17_Chr4g0054131 39.015 264 132 5 1 248 1 251 7.15e-54 176
MsaG023942 MtrunA17_Chr5g0401061 41.810 232 130 3 1 231 1 228 2.28e-51 169
MsaG023942 MtrunA17_Chr8g0383841 88.235 68 8 0 186 253 1 68 5.06e-34 119
MsaG023942 MtrunA17_Chr1g0195251 51.969 127 49 3 125 251 295 409 6.12e-32 122
MsaG023942 MtrunA17_Chr8g0342871 37.245 196 102 5 1 187 1 184 7.18e-29 110
MsaG023942 MtrunA17_Chr2g0329921 32.877 219 102 5 1 187 1 206 4.62e-26 103
MsaG023942 MtrunA17_Chr1g0166681 67.188 64 21 0 1 64 1 64 3.99e-25 98.6
MsaG023942 MtrunA17_Chr1g0195261 74.000 50 13 0 1 50 1 50 4.41e-21 83.6
MsaG023942 MtrunA17_Chr2g0293401 61.290 62 24 0 61 122 3 64 1.41e-15 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023942 AT1G49950.3 46.899 258 111 9 1 250 1 240 7.66e-66 207
MsaG023942 AT1G49950.1 46.899 258 111 9 1 250 1 240 7.66e-66 207
MsaG023942 AT1G49950.2 46.899 258 111 9 1 250 1 240 7.66e-66 207
MsaG023942 AT5G67580.2 39.224 232 136 4 1 229 1 230 6.04e-45 153
MsaG023942 AT5G67580.1 39.224 232 136 4 1 229 1 230 6.04e-45 153
MsaG023942 AT3G49850.1 36.530 219 137 2 1 219 1 217 2.34e-40 141
MsaG023942 AT3G49850.2 36.530 219 137 2 1 219 1 217 2.34e-40 141
MsaG023942 AT1G72740.2 36.316 190 113 3 1 187 1 185 1.25e-28 110
MsaG023942 AT1G72740.1 36.957 184 108 3 1 181 1 179 2.70e-27 107
MsaG023942 AT1G17520.1 35.638 188 116 1 1 183 1 188 7.79e-25 100
MsaG023942 AT1G17520.4 34.737 190 119 1 1 185 1 190 2.35e-24 99.0
MsaG023942 AT1G17520.2 35.979 189 115 2 1 183 1 189 6.95e-24 97.8
MsaG023942 AT1G17520.3 35.079 191 118 2 1 185 1 191 2.23e-23 96.7

Find 67 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGATATGCTGGAAATTA+AGG 0.140872 4:-93966168 MsaT023942.1:CDS
AACTATTGCTTCTTTCATTA+AGG 0.194532 4:-93965219 MsaT023942.1:intron
AAACCGACCCATGTTATTAT+TGG 0.212298 4:-93962763 MsaT023942.1:CDS
ACTCTCAGCTAGCAATAAAA+AGG 0.232134 4:-93966292 MsaT023942.1:CDS
TAGATATTGCTTCCACTATA+AGG 0.286103 4:+93965273 None:intergenic
TGTGGGTTGGATAGGATAAA+TGG 0.326262 4:-93966358 MsaT023942.1:intron
TGAAGCATGGCGTAGGTAAA+TGG 0.346059 4:-93966558 MsaT023942.1:CDS
TTCCTCTATCTGAATATGCT+TGG 0.349369 4:+93962787 None:intergenic
AAATAAGCTGTCAGCAAAAC+TGG 0.353881 4:-93963775 MsaT023942.1:CDS
TCCTCTATCTGAATATGCTT+GGG 0.366075 4:+93962788 None:intergenic
GGCTGAGATAGACTTGGAAT+TGG 0.370284 4:-93962655 MsaT023942.1:CDS
CTATTAAATCTGACAGGGGT+GGG 0.403434 4:-93962722 MsaT023942.1:CDS
ACAGATATGCTGGAAATTAA+GGG 0.415666 4:-93966167 MsaT023942.1:CDS
GAGGAAGCGGCTCTCAAAGC+TGG 0.427705 4:-93966586 MsaT023942.1:CDS
ACGGAGTCGTCACGCTGTTC+TGG 0.440395 4:+93966254 None:intergenic
TATTAAATCTGACAGGGGTG+GGG 0.448513 4:-93962721 MsaT023942.1:CDS
TCAAGTTAAAACAGATATGC+TGG 0.453383 4:-93966177 MsaT023942.1:CDS
AATCTGCCGGTGCGCAGTAT+TGG 0.455578 4:+93963802 None:intergenic
ATTAAATCTGACAGGGGTGG+GGG 0.463232 4:-93962720 MsaT023942.1:CDS
AAGCCTCTATTAAATCTGAC+AGG 0.463302 4:-93962728 MsaT023942.1:CDS
TTCACACTTCAATCAAGAGC+AGG 0.468692 4:+93962102 None:intergenic
AATATGAGTCTGAAGGCAAA+TGG 0.474909 4:-93966332 MsaT023942.1:CDS
ACCCAAGCATATTCAGATAG+AGG 0.477513 4:-93962789 MsaT023942.1:CDS
AGGCAAATGGATCATCCTCC+GGG 0.485206 4:-93966319 MsaT023942.1:CDS
ACCCCTGTCAGATTTAATAG+AGG 0.496957 4:+93962725 None:intergenic
CCTCCCTTCCAATAATAACA+TGG 0.497696 4:+93962755 None:intergenic
AAGGCAAATGGATCATCCTC+CGG 0.503120 4:-93966320 MsaT023942.1:CDS
CAGATTGGACAACCTTATAG+TGG 0.503998 4:-93965285 MsaT023942.1:CDS
CTGGTGGATCTTGTATCTAG+TGG 0.509226 4:-93963756 MsaT023942.1:CDS
GTCCGGAAAGGTCTATCAAA+AGG 0.511188 4:-93966145 MsaT023942.1:intron
TATGCTGGAAATTAAGGGTC+CGG 0.513448 4:-93966162 MsaT023942.1:CDS
TTATATTTCAGGTGAAACAT+AGG 0.516350 4:-93962827 MsaT023942.1:intron
CGACCCATGTTATTATTGGA+AGG 0.524027 4:-93962759 MsaT023942.1:CDS
ATCTAGTGGAAAACTGATCA+AGG 0.537966 4:-93963742 MsaT023942.1:intron
AAAGTCCATGTCTTTACAAG+AGG 0.539777 4:-93962625 MsaT023942.1:intron
CCGCTCAAATATCGATCTCA+AGG 0.560055 4:-93966491 MsaT023942.1:intron
AAGAAAGGCTGAGATAGACT+TGG 0.566957 4:-93962661 MsaT023942.1:CDS
ATGGTCATCAGAAGAGGAAG+CGG 0.577986 4:-93966599 MsaT023942.1:CDS
ACAGAAATGGTCATCAGAAG+AGG 0.578108 4:-93966605 MsaT023942.1:CDS
AGCAATATCTAGCTTGAATG+AGG 0.580943 4:-93965261 MsaT023942.1:CDS
GACCCATGTTATTATTGGAA+GGG 0.581327 4:-93962758 MsaT023942.1:CDS
CTATCTGAATATGCTTGGGT+AGG 0.584721 4:+93962792 None:intergenic
ATGGAGAAATATGAGTCTGA+AGG 0.599241 4:-93966339 MsaT023942.1:CDS
TGGGTGCTACTAGACAGAAA+TGG 0.601772 4:-93966618 MsaT023942.1:CDS
AGCCTCTATTAAATCTGACA+GGG 0.601960 4:-93962727 MsaT023942.1:CDS
CTTCCAATAATAACATGGGT+CGG 0.603083 4:+93962760 None:intergenic
CCATGTTATTATTGGAAGGG+AGG 0.603196 4:-93962755 MsaT023942.1:CDS
GGAGTTGTGAAGCATGGCGT+AGG 0.603718 4:-93966565 MsaT023942.1:CDS
TGCTAGCTGAGAGTTGTCCC+CGG 0.606787 4:+93966301 None:intergenic
TCACACTTCAATCAAGAGCA+GGG 0.606896 4:+93962103 None:intergenic
CTCCCTTCCAATAATAACAT+GGG 0.607660 4:+93962756 None:intergenic
TCTATTAAATCTGACAGGGG+TGG 0.615717 4:-93962723 MsaT023942.1:CDS
AAAGCTGGAGTTGTGAAGCA+TGG 0.620365 4:-93966571 MsaT023942.1:CDS
TAAGCTGTCAGCAAAACTGG+TGG 0.625095 4:-93963772 MsaT023942.1:CDS
TACAACACATGGTTAAACTC+AGG 0.630999 4:+93966517 None:intergenic
CCTTGAGATCGATATTTGAG+CGG 0.641849 4:+93966491 None:intergenic
TGGAAATTAAGGGTCCGGAA+AGG 0.649175 4:-93966157 MsaT023942.1:CDS
TCTAGCTTGAATGAGGTCGA+TGG 0.649394 4:-93965254 MsaT023942.1:CDS
GTAGAACCAATACTGCGCAC+CGG 0.653755 4:-93963808 MsaT023942.1:intron
GATTACCTCTTGTAAAGACA+TGG 0.659824 4:+93962620 None:intergenic
GCCTCTATTAAATCTGACAG+GGG 0.668262 4:-93962726 MsaT023942.1:CDS
TAAATGGAGTAAGATACTCA+AGG 0.669197 4:-93966542 MsaT023942.1:CDS
AGTAACAAGATTTACAGCCA+CGG 0.669902 4:+93966235 None:intergenic
TTGAGCGGATATACAACACA+TGG 0.677687 4:+93966506 None:intergenic
AGAACAGCGTGACGACTCCG+TGG 0.703510 4:-93966252 MsaT023942.1:CDS
TAGCTGAGAGTTGTCCCCGG+AGG 0.710409 4:+93966304 None:intergenic
GGCAAATGGATCATCCTCCG+GGG 0.731603 4:-93966318 MsaT023942.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 93962110 93966641 93962110 ID=MsaG023942
Chr4 mRNA 93962110 93966641 93962110 ID=MsaT023942.1;Parent=MsaG023942
Chr4 exon 93962110 93962130 93962110 ID=MsaT023942.1.exon6;Parent=MsaT023942.1
Chr4 CDS 93962110 93962130 93962110 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Chr4 exon 93962626 93962838 93962626 ID=MsaT023942.1.exon5;Parent=MsaT023942.1
Chr4 CDS 93962626 93962838 93962626 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Chr4 exon 93963743 93963826 93963743 ID=MsaT023942.1.exon4;Parent=MsaT023942.1
Chr4 CDS 93963743 93963826 93963743 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Chr4 exon 93965220 93965304 93965220 ID=MsaT023942.1.exon3;Parent=MsaT023942.1
Chr4 CDS 93965220 93965304 93965220 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Chr4 exon 93966146 93966366 93966146 ID=MsaT023942.1.exon2;Parent=MsaT023942.1
Chr4 CDS 93966146 93966366 93966146 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Chr4 exon 93966492 93966641 93966492 ID=MsaT023942.1.exon1;Parent=MsaT023942.1
Chr4 CDS 93966492 93966641 93966492 ID=cds.MsaT023942.1;Parent=MsaT023942.1
Gene Sequence

>MsaT023942.1

ATGGGTGCTACTAGACAGAAATGGTCATCAGAAGAGGAAGCGGCTCTCAAAGCTGGAGTTGTGAAGCATGGCGTAGGTAAATGGAGTAAGATACTCAAGGATCCTGAGTTTAACCATGTGTTGTATATCCGCTCAAATATCGATCTCAAGGATAAATGGAGAAATATGAGTCTGAAGGCAAATGGATCATCCTCCGGGGACAACTCTCAGCTAGCAATAAAAAGGGTGCGTCATCAGGCACCAGAACAGCGTGACGACTCCGTGGCTGTAAATCTTGTTACTACTATTGATGATGAAATTCTTGATGTTCAGCCTCTTCAAGTTAAAACAGATATGCTGGAAATTAAGGGTCCGGAAAGGTCTATCAAAAGATTGGACAACCTTATAGTGGAAGCAATATCTAGCTTGAATGAGGTCGATGGTTCTAATAAAACAACTATTGCTTCTTTCATTAAGAACCAATACTGCGCACCGGCAGATTTCAAAAATAAGCTGTCAGCAAAACTGGTGGATCTTGTATCTAGTGGAAAACTGATCAAGGTGAAACATAGGTACATGATTACGCCTACCCAAGCATATTCAGATAGAGGAAGAAACCGACCCATGTTATTATTGGAAGGGAGGCAGAAAGCCTCTATTAAATCTGACAGGGGTGGGGGCAATATCCCCAAAAAGTATGACATAGATTATCAAAAAAGAAAGGCTGAGATAGACTTGGAATTGGAAAAGTTAAAGTCCATGTCTTTACAAGAGATGATCCCTGCTCTTGATTGA

Protein sequence

>MsaT023942.1

MGATRQKWSSEEEAALKAGVVKHGVGKWSKILKDPEFNHVLYIRSNIDLKDKWRNMSLKANGSSSGDNSQLAIKRVRHQAPEQRDDSVAVNLVTTIDDEILDVQPLQVKTDMLEIKGPERSIKRLDNLIVEAISSLNEVDGSNKTTIASFIKNQYCAPADFKNKLSAKLVDLVSSGKLIKVKHRYMITPTQAYSDRGRNRPMLLLEGRQKASIKSDRGGGNIPKKYDIDYQKRKAEIDLELEKLKSMSLQEMIPALD*