AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023972


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023972 MtrunA17_Chr8g0384141 93.878 245 13 1 1 243 21 265 1.05e-148 415
MsaG023972 MtrunA17_Chr3g0134001 73.958 96 25 0 60 155 139 234 4.44e-47 157
MsaG023972 MtrunA17_Chr8g0375121 68.571 105 33 0 60 164 100 204 4.53e-38 133
MsaG023972 MtrunA17_Chr5g0402161 64.545 110 39 0 60 169 105 214 1.01e-37 132
MsaG023972 MtrunA17_Chr8g0341421 60.577 104 36 2 58 156 289 392 5.51e-29 113
MsaG023972 MtrunA17_Chr2g0330641 62.105 95 35 1 63 156 260 354 6.39e-27 107
MsaG023972 MtrunA17_Chr7g0243041 67.857 56 18 0 98 153 32 87 9.73e-21 85.5
MsaG023972 MtrunA17_Chr5g0435021 45.455 99 52 2 58 154 182 280 1.39e-19 85.9
MsaG023972 MtrunA17_Chr1g0152651 66.071 56 19 0 101 156 283 338 5.78e-19 84.7
MsaG023972 MtrunA17_Chr5g0393721 66.071 56 19 0 101 156 233 288 5.99e-19 84.3
MsaG023972 MtrunA17_Chr1g0207301 41.176 102 59 1 56 156 251 352 1.23e-18 84.0
MsaG023972 MtrunA17_Chr1g0207311 66.071 56 19 0 101 156 205 260 2.05e-18 82.0
MsaG023972 MtrunA17_Chr3g0134741 60.606 66 25 1 92 156 228 293 5.10e-18 81.6
MsaG023972 MtrunA17_Chr4g0074161 50.667 75 37 0 80 154 194 268 1.43e-15 75.1
MsaG023972 MtrunA17_Chr1g0179061 59.259 54 22 0 102 155 180 233 2.01e-14 70.5
MsaG023972 MtrunA17_Chr1g0190731 50.000 70 35 0 85 154 186 255 1.33e-13 69.3
MsaG023972 MtrunA17_Chr2g0311101 44.737 76 40 1 82 155 157 232 1.79e-13 68.6
MsaG023972 MtrunA17_Chr2g0323091 52.632 57 27 0 102 158 146 202 2.24e-13 68.2
MsaG023972 MtrunA17_Chr4g0014771 59.259 54 21 1 102 154 128 181 2.12e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023972 AT5G09750.1 81.651 109 13 1 65 166 92 200 1.25e-46 155
MsaG023972 AT4G00120.1 82.022 89 16 0 67 155 87 175 2.16e-46 153
MsaG023972 AT3G21330.1 52.632 133 56 1 56 188 233 358 7.68e-38 136
MsaG023972 AT5G67060.2 75.824 91 22 0 65 155 83 173 4.25e-36 128
MsaG023972 AT5G67060.1 75.824 91 22 0 65 155 95 185 6.44e-36 127
MsaG023972 AT3G50330.1 74.725 91 23 0 65 155 92 182 2.04e-35 126
MsaG023972 AT5G01305.1 64.179 67 24 0 90 156 35 101 3.75e-21 87.4
MsaG023972 AT2G14760.1 67.857 56 18 0 101 156 246 301 6.19e-19 84.7
MsaG023972 AT5G43175.1 47.500 80 42 0 77 156 117 196 6.65e-19 82.8
MsaG023972 AT4G33880.1 67.857 56 18 0 101 156 275 330 7.04e-19 84.7
MsaG023972 AT1G27740.1 50.704 71 35 0 86 156 161 231 1.13e-17 80.1
MsaG023972 AT1G66470.1 64.815 54 19 0 101 154 204 257 1.33e-17 80.5
MsaG023972 AT5G37800.1 52.778 72 26 1 83 154 200 263 1.65e-17 80.5
MsaG023972 AT4G30980.1 59.091 66 26 1 90 154 127 192 1.94e-16 77.4
MsaG023972 AT4G30980.2 59.091 66 26 1 90 154 175 240 3.98e-16 77.0
MsaG023972 AT2G24260.2 62.963 54 20 0 101 154 44 97 4.55e-16 75.5
MsaG023972 AT2G24260.1 49.333 75 32 1 86 154 126 200 9.26e-16 75.9
MsaG023972 AT2G14760.3 55.072 69 18 1 101 156 246 314 1.46e-15 75.1
MsaG023972 AT5G58010.1 52.113 71 33 1 85 154 91 161 5.43e-15 73.2

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGAATTCTTCTGGAATT+TGG 0.166878 4:+94319382 MsaT023972.1:CDS
GGTTCACTCAGTGACATATT+AGG 0.250977 4:+94319456 MsaT023972.1:CDS
TTGTTATTGTCTCCTTCTTC+TGG 0.266342 4:+94319873 MsaT023972.1:CDS
TGTTCCTGGTGGAACTAAAA+TGG 0.289003 4:+94319698 MsaT023972.1:CDS
CAAGTTCCTATGAATTCTTC+TGG 0.298023 4:+94319375 MsaT023972.1:CDS
ATCATGCTGTTGTAGCTATT+TGG 0.316175 4:-94319408 None:intergenic
CTTCTTCTGGTTGTGACGTC+TGG 0.336110 4:+94319886 MsaT023972.1:CDS
GTGGAAGTGAGTAGTAAATT+TGG 0.351622 4:-94319918 None:intergenic
ATTCGAACGTTTCTTCGCTT+TGG 0.353088 4:-94319597 None:intergenic
TGTCTAGCTGCAACACTTTG+TGG 0.381712 4:-94319630 None:intergenic
ATCCTTCAAAGACTTGTTCC+TGG 0.399593 4:+94319684 MsaT023972.1:CDS
GAAGAACAAGAAGAAGAGTT+AGG 0.401481 4:+94319501 MsaT023972.1:CDS
AGAAGAGTTAGGAGCTATGA+AGG 0.405429 4:+94319512 MsaT023972.1:CDS
GTTCACTCAGTGACATATTA+GGG 0.414540 4:+94319457 MsaT023972.1:CDS
GATCTTGTTGGAGAATAATA+CGG 0.432514 4:-94319347 None:intergenic
AGTAGTAAATTTGGTGCAAA+AGG 0.442540 4:-94319909 None:intergenic
GTTCTTCTACATCTTCGATT+CGG 0.446833 4:-94319485 None:intergenic
TCCGGCGTCAAATTGAATTC+CGG 0.450878 4:-94319967 None:intergenic
CAAATTCCAGAAGAATTCAT+AGG 0.451806 4:-94319381 None:intergenic
CAGAAGAATTCATAGGAACT+TGG 0.471897 4:-94319374 None:intergenic
CACCAGGAACAAGTCTTTGA+AGG 0.481487 4:-94319686 None:intergenic
GAACTTGGTGGTGATCTTGT+TGG 0.483925 4:-94319359 None:intergenic
CTTCTACATCTTCGATTCGG+TGG 0.493447 4:-94319482 None:intergenic
CAAGATTGCAGCTATGCAAC+CGG 0.495861 4:+94319545 MsaT023972.1:CDS
GCACCACCACCACCTTCATC+AGG 0.499844 4:+94319435 MsaT023972.1:CDS
GTCCACTGTGGCAGCTGTTG+TGG 0.509617 4:-94319937 None:intergenic
CAGACGTCACAACCAGAAGA+AGG 0.532660 4:-94319885 None:intergenic
TAGCTACAACAGCATGATCA+TGG 0.539866 4:+94319413 MsaT023972.1:CDS
ATGCTGTTGTAGCTATTTGG+TGG 0.542016 4:-94319405 None:intergenic
GAACCTGATGAAGGTGGTGG+TGG 0.552409 4:-94319438 None:intergenic
ATGAACATGTGAATGTCCTC+CGG 0.556908 4:-94319985 None:intergenic
GGCGTCAAATTGAATTCCGG+TGG 0.574382 4:-94319964 None:intergenic
CAAGAACTTAACATAACGAA+TGG 0.583966 4:-94319745 None:intergenic
TCACATGTTCATGCTTGTGA+AGG 0.591006 4:+94319996 MsaT023972.1:CDS
GCCACAGTGGACTTACCCAC+CGG 0.591865 4:+94319948 MsaT023972.1:CDS
AAGAATTCATAGGAACTTGG+TGG 0.592411 4:-94319371 None:intergenic
ACTCACTAATTACCTCATGA+TGG 0.592814 4:-94320034 None:intergenic
GACAATAACAAAGCACTAGG+AGG 0.606861 4:-94319861 None:intergenic
GGAGACAATAACAAAGCACT+AGG 0.609214 4:-94319864 None:intergenic
GGAATTCAATTTGACGCCGG+AGG 0.622262 4:+94319969 MsaT023972.1:CDS
CTTCAAAGACTTGTTCCTGG+TGG 0.627271 4:+94319687 MsaT023972.1:CDS
GTTGCTGGGTCGATATCCAC+CGG 0.629556 4:-94319564 None:intergenic
ACCGGAATTCAATTTGACGC+CGG 0.643580 4:+94319966 MsaT023972.1:CDS
ATCAAGCTTTAACCATCATG+AGG 0.658321 4:+94320022 MsaT023972.1:CDS
TTAATGCATTGTGATGAAAG+TGG 0.662271 4:-94319831 None:intergenic
TCACTGAGTGAACCTGATGA+AGG 0.669394 4:-94319447 None:intergenic
GATTGCAGCTATGCAACCGG+TGG 0.671341 4:+94319548 MsaT023972.1:CDS
GCGTCAAATTGAATTCCGGT+GGG 0.674022 4:-94319963 None:intergenic
TTCCACAACAGCTGCCACAG+TGG 0.676021 4:+94319935 MsaT023972.1:CDS
AGTGAACCTGATGAAGGTGG+TGG 0.679836 4:-94319441 None:intergenic
CTGAGTGAACCTGATGAAGG+TGG 0.711599 4:-94319444 None:intergenic
TCCGGTGGGTAAGTCCACTG+TGG 0.759981 4:-94319949 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 94319327 94320058 94319327 ID=MsaG023972
Chr4 mRNA 94319327 94320058 94319327 ID=MsaT023972.1;Parent=MsaG023972
Chr4 exon 94319327 94320058 94319327 ID=MsaT023972.1.exon1;Parent=MsaT023972.1
Chr4 CDS 94319327 94320058 94319327 ID=cds.MsaT023972.1;Parent=MsaT023972.1
Gene Sequence

>MsaT023972.1

ATGGAAACAAATGAACAAAACCGTATTATTCTCCAACAAGATCACCACCAAGTTCCTATGAATTCTTCTGGAATTTGGCCACCAAATAGCTACAACAGCATGATCATGGCACCACCACCACCTTCATCAGGTTCACTCAGTGACATATTAGGGATCCACCGAATCGAAGATGTAGAAGAACAAGAAGAAGAGTTAGGAGCTATGAAGGAAATGATGTACAAGATTGCAGCTATGCAACCGGTGGATATCGACCCAGCAACGATTCGAAAACCAAAGCGAAGAAACGTTCGAATCAGCGACGATCCACAAAGTGTTGCAGCTAGACATAGAAGAGAAAGAATCAGTGAGAAGATTCGTATCCTTCAAAGACTTGTTCCTGGTGGAACTAAAATGGATACAGCTTCTATGCTTGATGAAGCCATTCGTTATGTTAAGTTCTTGAAGAGACAAATCAAGTTGCTTCAATCCACTCCTCAAAATCAACAACAACAACACATTCAATTGCCACTTTCATCACAATGCATTAATTCTACTCCTCCTAGTGCTTTGTTATTGTCTCCTTCTTCTGGTTGTGACGTCTGGCCTTTTGCACCAAATTTACTACTCACTTCCACAACAGCTGCCACAGTGGACTTACCCACCGGAATTCAATTTGACGCCGGAGGACATTCACATGTTCATGCTTGTGAAGGCTCATCAAGCTTTAACCATCATGAGGTAATTAGTGAGTGA

Protein sequence

>MsaT023972.1

METNEQNRIILQQDHHQVPMNSSGIWPPNSYNSMIMAPPPPSSGSLSDILGIHRIEDVEEQEEELGAMKEMMYKIAAMQPVDIDPATIRKPKRRNVRISDDPQSVAARHRRERISEKIRILQRLVPGGTKMDTASMLDEAIRYVKFLKRQIKLLQSTPQNQQQQHIQLPLSSQCINSTPPSALLLSPSSGCDVWPFAPNLLLTSTTAATVDLPTGIQFDAGGHSHVHACEGSSSFNHHEVISE*