AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG023990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023990 MtrunA17_Chr8g0384351 89.894 188 8 4 1 181 2 185 3.34e-116 327
MsaG023990 MtrunA17_Chr3g0130321 51.707 205 70 9 6 181 4 208 9.73e-58 180
MsaG023990 MtrunA17_Chr4g0050701 46.561 189 77 6 9 181 11 191 1.47e-48 156
MsaG023990 MtrunA17_Chr4g0042561 39.062 192 94 7 1 172 1 189 3.72e-32 116
MsaG023990 MtrunA17_Chr2g0318101 37.569 181 84 6 9 160 15 195 7.45e-31 114
MsaG023990 MtrunA17_Chr8g0384721 42.405 158 72 6 4 142 2 159 1.12e-29 110
MsaG023990 MtrunA17_Chr7g0263931 39.306 173 82 6 5 155 7 178 1.31e-29 110
MsaG023990 MtrunA17_Chr5g0417791 37.778 180 91 6 7 165 1 180 6.78e-29 107
MsaG023990 MtrunA17_Chr3g0124761 36.872 179 95 5 5 165 3 181 2.12e-28 107
MsaG023990 MtrunA17_Chr7g0214071 38.462 169 73 7 7 153 13 172 6.44e-28 105
MsaG023990 MtrunA17_Chr2g0326361 36.842 171 86 5 2 150 7 177 5.15e-27 103
MsaG023990 MtrunA17_Chr4g0047841 37.853 177 69 6 9 144 15 191 1.49e-26 103
MsaG023990 MtrunA17_Chr3g0130731 38.854 157 78 5 4 142 2 158 1.86e-26 102
MsaG023990 MtrunA17_Chr7g0252971 37.647 170 76 7 1 141 1 169 2.54e-26 102
MsaG023990 MtrunA17_Chr3g0131911 37.805 164 74 7 8 145 13 174 3.37e-25 97.4
MsaG023990 MtrunA17_Chr2g0318081 37.342 158 83 4 5 146 12 169 3.47e-25 99.8
MsaG023990 MtrunA17_Chr8g0359921 38.415 164 75 6 5 146 10 169 1.38e-23 95.1
MsaG023990 MtrunA17_Chr3g0091921 36.810 163 85 5 9 153 16 178 1.79e-23 94.4
MsaG023990 MtrunA17_Chr1g0147421 40.132 152 70 5 9 141 27 176 3.14e-23 94.4
MsaG023990 MtrunA17_Chr3g0139331 36.471 170 88 5 9 160 11 178 6.33e-23 93.6
MsaG023990 MtrunA17_Chr6g0484441 36.842 152 78 5 9 144 5 154 6.96e-23 93.2
MsaG023990 MtrunA17_Chr4g0060931 38.312 154 71 6 9 141 17 167 1.18e-22 92.8
MsaG023990 MtrunA17_Chr6g0481091 37.419 155 79 5 5 141 2 156 1.58e-22 90.5
MsaG023990 MtrunA17_Chr3g0109821 38.217 157 72 7 7 141 4 157 1.87e-22 93.6
MsaG023990 MtrunA17_Chr8g0385991 34.810 158 82 6 8 144 13 170 2.93e-22 89.7
MsaG023990 MtrunA17_Chr4g0017921 32.474 194 104 8 3 172 2 192 1.57e-21 89.7
MsaG023990 MtrunA17_Chr5g0433781 36.774 155 80 5 5 141 2 156 2.12e-21 90.5
MsaG023990 MtrunA17_Chr2g0309331 33.333 171 92 7 1 151 2 170 2.96e-21 89.0
MsaG023990 MtrunA17_Chr1g0183741 35.583 163 77 7 7 148 8 163 1.89e-20 85.9
MsaG023990 MtrunA17_Chr2g0309891 31.319 182 104 7 5 165 2 183 1.93e-20 86.7
MsaG023990 MtrunA17_Chr6g0453451 33.728 169 94 5 3 153 14 182 3.01e-20 85.9
MsaG023990 MtrunA17_Chr3g0130101 36.667 150 77 5 11 142 9 158 3.04e-20 86.3
MsaG023990 MtrunA17_Chr8g0372121 34.940 166 85 7 9 154 6 168 3.99e-20 85.5
MsaG023990 MtrunA17_Chr7g0253011 31.841 201 107 6 2 180 10 202 4.10e-20 86.3
MsaG023990 MtrunA17_Chr2g0312321 36.364 154 75 7 7 141 9 158 7.09e-20 84.7
MsaG023990 MtrunA17_Chr7g0261411 36.364 154 70 7 7 139 8 154 1.47e-19 83.6
MsaG023990 MtrunA17_Chr2g0316971 37.179 156 72 6 9 141 23 175 2.74e-19 84.0
MsaG023990 MtrunA17_Chr5g0405211 32.143 196 97 9 9 176 8 195 3.16e-19 83.6
MsaG023990 MtrunA17_Chr4g0018391 32.995 197 99 9 9 181 7 194 3.83e-19 83.2
MsaG023990 MtrunA17_Chr8g0346001 31.902 163 83 6 8 142 14 176 5.30e-19 83.2
MsaG023990 MtrunA17_Chr5g0398971 35.032 157 79 7 9 144 7 161 1.10e-18 82.0
MsaG023990 MtrunA17_Chr1g0197351 33.758 157 80 6 9 141 42 198 1.46e-18 82.4
MsaG023990 MtrunA17_Chr6g0453131 33.537 164 79 7 9 144 15 176 2.40e-18 81.3
MsaG023990 MtrunA17_Chr2g0311651 30.120 166 84 6 11 144 24 189 4.54e-18 79.7
MsaG023990 MtrunA17_Chr4g0056541 34.194 155 81 6 9 144 7 159 7.40e-18 79.7
MsaG023990 MtrunA17_Chr2g0311661 30.357 168 85 7 11 146 23 190 1.04e-17 78.6
MsaG023990 MtrunA17_Chr4g0054791 30.939 181 104 5 7 166 4 184 1.07e-17 79.0
MsaG023990 MtrunA17_Chr8g0384201 32.026 153 86 5 8 142 5 157 4.42e-17 78.2
MsaG023990 MtrunA17_Chr1g0200561 34.969 163 77 8 1 141 13 168 7.93e-17 76.6
MsaG023990 MtrunA17_Chr5g0417001 32.919 161 80 7 11 144 16 175 9.24e-17 76.6
MsaG023990 MtrunA17_Chr5g0442771 29.268 164 88 6 11 146 20 183 9.28e-17 76.3
MsaG023990 MtrunA17_Chr6g0461661 34.375 160 83 6 3 141 12 170 9.31e-17 76.6
MsaG023990 MtrunA17_Chr3g0144271 36.538 156 73 5 9 145 23 171 1.55e-16 76.3
MsaG023990 MtrunA17_Chr7g0217721 32.934 167 81 5 7 142 17 183 1.61e-16 75.9
MsaG023990 MtrunA17_Chr8g0364791 32.026 153 86 5 8 142 5 157 1.61e-16 76.3
MsaG023990 MtrunA17_Chr5g0428991 32.680 153 85 5 8 142 24 176 1.92e-16 76.3
MsaG023990 MtrunA17_Chr5g0400461 30.387 181 98 6 1 156 1 178 2.90e-16 75.1
MsaG023990 MtrunA17_Chr4g0076731 33.987 153 72 6 11 141 56 201 5.96e-16 74.7
MsaG023990 MtrunA17_Chr8g0345671 34.667 150 73 7 9 138 11 155 6.10e-16 74.7
MsaG023990 MtrunA17_Chr4g0038751 33.333 168 78 8 11 152 17 176 1.06e-15 73.2
MsaG023990 MtrunA17_Chr3g0092671 31.646 158 88 6 6 144 2 158 2.36e-15 73.2
MsaG023990 MtrunA17_Chr7g0217711 35.075 134 71 4 7 124 17 150 4.64e-15 69.3
MsaG023990 MtrunA17_Chr3g0128151 32.450 151 84 5 9 141 22 172 6.07e-15 72.0
MsaG023990 MtrunA17_Chr8g0345731 33.333 132 65 5 9 117 24 155 6.56e-14 66.2
MsaG023990 MtrunA17_Chr3g0128111 30.405 148 85 5 12 141 45 192 2.98e-13 65.9
MsaG023990 MtrunA17_Chr8g0345691 30.000 150 80 6 9 138 12 156 5.08e-13 66.2
MsaG023990 MtrunA17_Chr2g0321631 32.692 156 79 8 10 139 5 160 7.95e-13 64.7
MsaG023990 MtrunA17_Chr8g0345711 31.008 129 69 4 9 117 12 140 3.19e-11 58.9
MsaG023990 MtrunA17_Chr4g0026141 30.128 156 84 7 9 139 5 160 3.97e-11 60.1
MsaG023990 MtrunA17_Chr8g0345701 30.137 146 70 4 4 117 2 147 9.13e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG023990 AT5G64530.1 46.667 195 75 6 7 180 1 187 1.29e-49 159
MsaG023990 AT5G64530.2 51.261 119 50 3 7 118 1 118 5.31e-36 123
MsaG023990 AT1G61110.1 41.317 167 69 6 9 146 16 182 3.81e-29 110
MsaG023990 AT1G77450.1 41.250 160 76 5 1 142 2 161 7.64e-29 107
MsaG023990 AT1G69490.1 37.766 188 89 7 1 168 1 180 1.62e-28 107
MsaG023990 AT2G33480.1 40.506 158 75 5 7 148 13 167 1.69e-28 107
MsaG023990 AT2G33480.2 41.772 158 71 7 7 148 13 165 1.90e-28 107
MsaG023990 AT1G01720.1 40.625 160 66 6 7 142 5 159 1.65e-27 105
MsaG023990 AT3G04070.2 37.931 174 76 7 9 153 10 180 8.03e-26 101
MsaG023990 AT5G63790.1 38.994 159 77 5 4 142 45 203 1.32e-25 100
MsaG023990 AT5G63790.2 38.994 159 77 5 4 142 55 213 1.48e-25 100
MsaG023990 AT1G52890.1 35.503 169 89 5 5 157 10 174 2.31e-25 100
MsaG023990 AT3G15500.1 33.918 171 97 4 5 159 10 180 4.58e-25 99.0
MsaG023990 AT3G18400.1 37.580 157 79 6 9 148 5 159 8.34e-25 98.6
MsaG023990 AT5G39610.1 38.125 160 75 6 4 142 15 171 1.12e-24 97.4
MsaG023990 AT5G13180.1 39.744 156 72 6 7 144 12 163 2.15e-24 96.3
MsaG023990 AT5G08790.1 38.994 159 77 5 4 142 2 160 2.84e-24 96.3
MsaG023990 AT4G27410.2 34.634 205 105 8 5 181 10 213 5.14e-24 95.9
MsaG023990 AT3G15510.1 32.821 195 96 7 13 178 21 209 1.11e-23 96.3
MsaG023990 AT5G61430.1 37.736 159 75 6 4 141 11 166 1.37e-23 95.5
MsaG023990 AT3G04070.1 34.392 189 78 7 9 153 10 196 1.44e-23 95.9
MsaG023990 AT5G07680.1 38.961 154 70 6 9 141 17 167 1.57e-23 95.1
MsaG023990 AT5G07680.2 38.961 154 70 6 9 141 3 153 1.64e-23 95.1
MsaG023990 AT2G24430.2 40.260 154 67 6 9 141 16 165 4.88e-23 93.6
MsaG023990 AT2G24430.1 40.260 154 67 6 9 141 16 165 4.88e-23 93.6
MsaG023990 AT3G12977.1 32.530 166 90 6 7 155 16 176 9.74e-23 92.4
MsaG023990 AT3G17730.1 37.419 155 79 5 5 141 2 156 1.63e-22 90.9
MsaG023990 AT4G10350.1 35.404 161 85 5 1 142 1 161 3.79e-22 91.7
MsaG023990 AT5G18270.2 37.423 163 75 6 2 141 14 172 7.39e-22 90.9
MsaG023990 AT1G33280.1 35.849 159 80 6 3 142 2 157 1.28e-21 89.7
MsaG023990 AT3G04060.1 33.168 202 101 9 2 180 13 203 1.30e-21 90.1
MsaG023990 AT1G56010.2 34.839 155 81 5 7 141 17 171 1.55e-21 89.7
MsaG023990 AT5G18270.1 36.810 163 76 6 2 141 14 172 1.80e-21 89.7
MsaG023990 AT3G10480.2 35.484 155 82 5 5 141 23 177 2.97e-21 90.1
MsaG023990 AT4G27410.3 32.432 222 104 9 5 181 10 230 3.48e-21 88.6
MsaG023990 AT1G79580.3 35.838 173 82 8 1 147 3 172 4.46e-21 89.0
MsaG023990 AT1G79580.5 35.838 173 82 8 1 147 3 172 4.46e-21 89.0
MsaG023990 AT1G79580.4 35.838 173 82 8 1 147 3 172 4.46e-21 89.0
MsaG023990 AT1G79580.2 35.838 173 82 8 1 147 3 172 4.46e-21 89.0
MsaG023990 AT1G79580.1 35.838 173 82 8 1 147 3 172 4.46e-21 89.0
MsaG023990 AT3G29035.1 41.406 128 59 4 7 118 22 149 7.73e-21 87.8
MsaG023990 AT5G53950.1 36.158 177 86 7 9 161 17 190 1.47e-20 87.8
MsaG023990 AT5G17260.1 35.256 156 82 6 5 141 2 157 1.60e-20 88.2
MsaG023990 AT5G04410.1 35.220 159 85 5 1 141 1 159 1.94e-20 88.2
MsaG023990 AT1G52880.1 36.875 160 73 6 13 144 21 180 2.11e-20 86.7
MsaG023990 AT3G03200.1 35.065 154 81 6 7 141 4 157 2.34e-20 87.8
MsaG023990 AT3G10480.1 35.849 159 77 7 5 141 23 178 2.38e-20 87.8
MsaG023990 AT3G15170.1 37.736 159 67 6 9 141 20 172 3.58e-20 85.9
MsaG023990 AT1G32770.1 32.317 164 82 6 8 142 15 178 4.58e-20 86.3
MsaG023990 AT1G65910.1 35.948 153 80 5 7 141 4 156 8.12e-20 86.3
MsaG023990 AT3G10490.1 33.548 155 84 6 6 141 24 178 1.03e-19 83.6
MsaG023990 AT3G10500.1 34.568 162 88 5 1 144 1 162 1.53e-19 85.5
MsaG023990 AT5G62380.2 32.500 160 87 6 4 144 2 159 1.76e-19 84.3
MsaG023990 AT5G62380.1 32.500 160 87 6 4 144 2 159 1.76e-19 84.3
MsaG023990 AT1G54330.1 34.839 155 80 6 7 143 4 155 4.32e-19 82.8
MsaG023990 AT1G76420.1 36.538 156 70 7 9 141 22 171 4.38e-19 83.2
MsaG023990 AT2G18060.3 32.121 165 91 6 6 151 6 168 6.57e-19 83.2
MsaG023990 AT2G18060.1 32.121 165 91 6 6 151 6 168 6.57e-19 83.2
MsaG023990 AT2G43000.1 30.128 156 92 5 11 149 20 175 6.83e-19 82.0
MsaG023990 AT3G10490.2 33.540 161 76 7 6 141 24 178 8.41e-19 83.2
MsaG023990 AT3G61910.1 33.133 166 79 8 8 141 10 175 8.70e-19 82.4
MsaG023990 AT4G35580.1 33.333 159 88 5 1 141 1 159 1.36e-18 82.8
MsaG023990 AT5G46590.1 35.404 161 81 7 1 141 1 158 1.36e-18 81.3
MsaG023990 AT4G36160.2 33.333 168 90 7 4 151 9 174 1.52e-18 82.0
MsaG023990 AT4G36160.1 33.333 168 90 7 4 151 5 170 1.69e-18 82.0
MsaG023990 AT4G36160.3 33.333 168 90 7 4 151 5 170 1.69e-18 82.0
MsaG023990 AT4G35580.3 33.333 159 88 5 1 141 1 159 1.80e-18 82.4
MsaG023990 AT4G35580.2 33.333 159 88 5 1 141 1 159 1.89e-18 82.4
MsaG023990 AT1G33060.2 33.113 151 83 5 9 141 24 174 2.28e-18 82.4
MsaG023990 AT1G33060.1 33.113 151 83 5 9 141 24 174 2.34e-18 82.0
MsaG023990 AT1G71930.2 31.847 157 86 6 9 146 9 163 7.31e-18 79.7
MsaG023990 AT1G71930.1 31.847 157 86 6 9 146 9 163 7.31e-18 79.7
MsaG023990 AT2G46770.1 30.994 171 86 6 8 146 15 185 1.07e-17 79.7
MsaG023990 AT1G12260.2 33.962 159 76 7 9 144 7 159 1.16e-17 79.3
MsaG023990 AT1G12260.1 33.962 159 76 7 9 144 7 159 2.17e-17 79.0
MsaG023990 AT1G62700.1 34.395 157 80 7 8 144 6 159 3.79e-17 78.2
MsaG023990 AT1G62700.2 34.395 157 80 7 8 144 6 159 3.79e-17 78.2
MsaG023990 AT3G12910.1 30.114 176 88 8 4 146 17 190 4.12e-17 77.4
MsaG023990 AT1G26870.1 33.537 164 74 7 9 141 16 175 9.01e-17 77.4
MsaG023990 AT5G46590.2 35.135 148 74 6 1 129 1 145 1.13e-16 75.9
MsaG023990 AT4G28530.1 32.544 169 74 7 8 141 9 172 1.20e-16 76.6
MsaG023990 AT5G66300.1 31.902 163 81 7 6 144 9 165 1.22e-16 75.9
MsaG023990 AT5G22290.1 29.114 158 89 5 11 151 23 174 1.36e-16 76.3
MsaG023990 AT1G32510.1 33.962 159 79 6 9 141 6 164 1.89e-16 75.5
MsaG023990 AT1G34190.1 32.022 178 94 7 11 161 18 195 2.56e-16 76.3
MsaG023990 AT2G17040.1 33.974 156 73 6 11 144 8 155 1.10e-15 73.2
MsaG023990 AT4G17980.1 36.691 139 65 6 1 119 1 136 1.28e-15 72.8
MsaG023990 AT4G17980.2 36.957 138 64 6 1 118 1 135 1.52e-15 73.2
MsaG023990 AT2G02450.1 32.432 148 81 5 11 141 53 198 2.55e-15 73.2
MsaG023990 AT5G24590.2 27.895 190 112 6 5 169 9 198 3.17e-15 73.2
MsaG023990 AT2G02450.2 32.432 148 81 5 11 141 53 198 3.24e-15 72.8
MsaG023990 AT3G49530.1 29.762 168 95 6 9 155 13 178 7.27e-15 72.0
MsaG023990 AT3G49530.2 29.762 168 95 6 9 155 13 178 8.86e-15 71.6
MsaG023990 AT1G02230.1 34.351 131 66 5 10 120 4 134 1.35e-14 70.9
MsaG023990 AT1G32870.1 35.714 154 81 6 9 144 10 163 8.99e-14 68.9
MsaG023990 AT1G32870.2 35.714 154 81 6 9 144 10 163 9.05e-14 68.9
MsaG023990 AT1G32870.3 35.714 154 81 6 9 144 46 199 1.07e-13 68.6
MsaG023990 AT5G14000.2 32.192 146 79 5 7 134 13 156 1.60e-13 65.1
MsaG023990 AT5G14000.1 28.977 176 102 6 7 164 13 183 1.74e-13 66.2
MsaG023990 AT1G34180.2 28.431 204 105 8 11 181 18 213 1.90e-13 68.2
MsaG023990 AT1G34180.4 28.431 204 105 8 11 181 18 213 2.76e-13 67.4
MsaG023990 AT5G50820.1 32.593 135 73 4 5 124 13 144 4.83e-13 64.7
MsaG023990 AT4G01520.1 31.013 158 81 6 7 141 2 154 1.04e-12 65.1
MsaG023990 AT4G01540.1 31.333 150 75 6 12 138 7 151 1.04e-12 65.9
MsaG023990 AT1G02250.1 33.113 151 73 7 10 138 4 148 1.15e-12 65.5
MsaG023990 AT4G01540.3 31.373 153 77 6 12 141 7 154 1.19e-12 65.5
MsaG023990 AT4G01540.2 31.333 150 75 6 12 138 7 151 1.55e-12 65.1
MsaG023990 AT4G01540.4 31.333 150 75 6 12 138 7 151 2.94e-12 64.3
MsaG023990 AT5G09330.5 29.412 153 90 5 7 141 4 156 3.80e-12 64.3
MsaG023990 AT5G09330.2 29.412 153 90 5 7 141 4 156 3.80e-12 64.3
MsaG023990 AT5G09330.4 29.412 153 90 5 7 141 4 156 3.80e-12 64.3
MsaG023990 AT5G09330.3 29.412 153 90 5 7 141 4 156 3.80e-12 64.3
MsaG023990 AT5G09330.1 29.412 153 90 5 7 141 4 156 3.80e-12 64.3
MsaG023990 AT4G01550.1 30.890 191 95 9 12 178 7 184 4.01e-12 63.9
MsaG023990 AT5G64060.1 29.801 151 88 5 9 141 6 156 3.10e-11 61.2
MsaG023990 AT3G12977.2 33.654 104 56 4 60 155 25 123 6.69e-11 59.3

Find 47 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAACCTATAAATAAAT+TGG 0.146052 4:-94503091 MsaT023990.1:CDS
TTCATTCCCAATTTATTTAT+AGG 0.164515 4:+94503084 None:intergenic
AATGGATATTGGAAGGAAAT+TGG 0.199332 4:-94503117 MsaT023990.1:CDS
AAGCTAATTATTAACTAATT+AGG 0.204594 4:+94499738 None:intergenic
GAAAATAGAAGCACTGAAAA+TGG 0.301344 4:-94503135 MsaT023990.1:CDS
ATGGATATTGGAAGGAAATT+GGG 0.330470 4:-94503116 MsaT023990.1:CDS
GTGTGAGACAGGATCATAAT+TGG 0.363529 4:-94499908 MsaT023990.1:intron
GAAGCACTGAAAATGGATAT+TGG 0.365813 4:-94503128 MsaT023990.1:CDS
TTGATCAAGATCATCATCTA+AGG 0.370976 4:+94499770 None:intergenic
TGGTGAGACAAGTGAAGTTC+AGG 0.398752 4:+94503844 None:intergenic
CATGATCCTTGGGAAATATA+CGG 0.400748 4:-94503823 MsaT023990.1:intron
AGTACCTTGTATTCAACCTT+GGG 0.405124 4:-94503059 MsaT023990.1:CDS
ACTTGTCTCACCATGATCCT+TGG 0.405810 4:-94503834 MsaT023990.1:CDS
AACAAAACACAATCACTTAC+CGG 0.413483 4:+94502985 None:intergenic
CTTGTCTCACCATGATCCTT+GGG 0.421667 4:-94503833 MsaT023990.1:CDS
GGTATGATGCTAGGATGATA+TGG 0.434256 4:+94503865 None:intergenic
GATGAGGATATTGATGATAA+TGG 0.434866 4:-94499828 MsaT023990.1:CDS
CTTCCCCAAGGTTGAATACA+AGG 0.441845 4:+94503055 None:intergenic
AGCAGTGTGAAGCTTCCTCC+TGG 0.444718 4:-94503952 MsaT023990.1:CDS
ACAAAACCTATAAATAAATT+GGG 0.452946 4:-94503090 MsaT023990.1:CDS
AATTGAAACAAGTTGGGTAA+TGG 0.457936 4:-94503031 MsaT023990.1:CDS
GGGAAGGAATTGAAACAAGT+TGG 0.476317 4:-94503038 MsaT023990.1:CDS
ATGATATGGTAAAGAAGCCT+TGG 0.489756 4:+94503879 None:intergenic
CGTATATTTCCCAAGGATCA+TGG 0.491411 4:+94503824 None:intergenic
GGAAGGAATTGAAACAAGTT+GGG 0.495348 4:-94503037 MsaT023990.1:CDS
ATATCATATTTCCTCATCTC+CGG 0.517814 4:-94503004 MsaT023990.1:CDS
AGGACTAGTTCTTCATCCGT+TGG 0.520052 4:+94503916 None:intergenic
AGGATCATAATTGGAGCAGA+TGG 0.520438 4:-94499899 MsaT023990.1:CDS
TAATTATTAACTAATTAGGT+AGG 0.520877 4:+94499742 None:intergenic
TGGATATTGGAAGGAAATTG+GGG 0.532640 4:-94503115 MsaT023990.1:CDS
AAGTACCTTGTATTCAACCT+TGG 0.541631 4:-94503060 MsaT023990.1:CDS
CTTGTATTCAACCTTGGGGA+AGG 0.542660 4:-94503054 MsaT023990.1:CDS
GCAGTGTGAAGCTTCCTCCT+GGG 0.560385 4:-94503951 MsaT023990.1:CDS
TGAAGTTCAGGTATGATGCT+AGG 0.561302 4:+94503856 None:intergenic
TTGTTTCAATTCCTTCCCCA+AGG 0.571160 4:+94503043 None:intergenic
GGATCATAATTGGAGCAGAT+GGG 0.571776 4:-94499898 MsaT023990.1:CDS
CCTTGGAATAGAGAAAATGA+AGG 0.572117 4:+94503896 None:intergenic
CACTGAAAATGGATATTGGA+AGG 0.575605 4:-94503124 MsaT023990.1:CDS
GAAAAGAAGATGTCACATCA+AGG 0.579347 4:-94499858 MsaT023990.1:CDS
ACAATCACTTACCGGAGATG+AGG 0.598757 4:+94502993 None:intergenic
GACATACCGTATATTTCCCA+AGG 0.621341 4:+94503817 None:intergenic
ACAGGTAAAGCATTGTCAAG+TGG 0.622513 4:-94503189 MsaT023990.1:intron
ACATCAAGGTGCAAGCGATG+AGG 0.654589 4:-94499844 MsaT023990.1:CDS
GTACCTTGTATTCAACCTTG+GGG 0.661844 4:-94503058 MsaT023990.1:CDS
ATAATGGAACAGATGTGTCA+TGG 0.669182 4:-94499812 MsaT023990.1:CDS
GTTGGATCAAAGTAAAACCC+AGG 0.688035 4:+94503934 None:intergenic
GGATCAAAGTAAAACCCAGG+AGG 0.734981 4:+94503937 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 94499752 94503986 94499752 ID=MsaG023990
Chr4 mRNA 94499752 94503986 94499752 ID=MsaT023990.1;Parent=MsaG023990
Chr4 exon 94499752 94499919 94499752 ID=MsaT023990.1.exon3;Parent=MsaT023990.1
Chr4 CDS 94499752 94499919 94499752 ID=cds.MsaT023990.1;Parent=MsaT023990.1
Chr4 exon 94502993 94503207 94502993 ID=MsaT023990.1.exon2;Parent=MsaT023990.1
Chr4 CDS 94502993 94503207 94502993 ID=cds.MsaT023990.1;Parent=MsaT023990.1
Chr4 exon 94503824 94503986 94503824 ID=MsaT023990.1.exon1;Parent=MsaT023990.1
Chr4 CDS 94503824 94503986 94503824 ID=cds.MsaT023990.1;Parent=MsaT023990.1
Gene Sequence

>MsaT023990.1

ATGGGGAGTGAAAGCAGTGTGAAGCTTCCTCCTGGGTTTTACTTTGATCCAACGGATGAAGAACTAGTCCTTCATTTTCTCTATTCCAAGGCTTCTTTACCATATCATCCTAGCATCATACCTGAACTTCACTTGTCTCACCATGATCCTTGGGAAATATACGGTAAAGCATTGTCAAGTGGAAATGATCACTACTTTTTCACCGAAGTGAAGGAAAATAGAAGCACTGAAAATGGATATTGGAAGGAAATTGGGGTCACAAAACCTATAAATAAATTGGGAATGAAGAAGTACCTTGTATTCAACCTTGGGGAAGGAATTGAAACAAGTTGGGTAATGGAAGAATATCATATTTCCTCATCTCCGGTAAGTGATTGTGATCATAATTGGAGCAGATGGGTTTTATGCAAAGTTTATGAAAAGAAGATGTCACATCAAGGTGCAAGCGATGAGGATATTGATGATAATGGAACAGATGTGTCATGGCTAGATGAAGTGTTTATGTCCTTAGATGATGATCTTGATCAAACAAGCCTACCTAATTAG
Protein sequence

>MsaT023990.1

MGSESSVKLPPGFYFDPTDEELVLHFLYSKASLPYHPSIIPELHLSHHDPWEIYGKALSSGNDHYFFTEVKENRSTENGYWKEIGVTKPINKLGMKKYLVFNLGEGIETSWVMEEYHISSSPVSDCDHNWSRWVLCKVYEKKMSHQGASDEDIDDNGTDVSWLDEVFMSLDDDLDQTSLPN*