AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024101 MtrunA17_Chr8g0385791 96.038 833 20 1 1 820 1 833 0.0 1656
MsaG024101 MtrunA17_Chr2g0276781 61.124 427 142 4 55 461 19 441 2.18e-177 526
MsaG024101 MtrunA17_Chr2g0276781 41.489 188 99 3 609 796 470 646 4.49e-35 142
MsaG024101 MtrunA17_Chr8g0346711 63.006 346 110 5 56 387 26 367 2.34e-147 449
MsaG024101 MtrunA17_Chr8g0346711 39.362 188 94 4 608 785 468 645 4.69e-27 117
MsaG024101 MtrunA17_Chr7g0239061 59.008 383 132 9 46 410 3 378 1.06e-144 442
MsaG024101 MtrunA17_Chr7g0239061 37.566 189 105 4 603 791 488 663 7.03e-31 129
MsaG024101 MtrunA17_Chr4g0009751 60.789 380 116 8 59 417 19 386 7.92e-143 435
MsaG024101 MtrunA17_Chr4g0009751 55.072 69 30 1 696 764 554 621 2.09e-17 86.7
MsaG024101 MtrunA17_Chr4g0029671 38.274 614 318 11 57 621 52 653 5.97e-129 404
MsaG024101 MtrunA17_Chr4g0029671 48.649 111 55 1 692 802 658 766 3.05e-29 125
MsaG024101 MtrunA17_Chr2g0326281 44.196 491 227 9 7 456 1 485 2.39e-123 390
MsaG024101 MtrunA17_Chr2g0326281 50.000 104 52 0 692 795 690 793 5.47e-30 127
MsaG024101 MtrunA17_Chr4g0046371 45.823 419 176 5 52 437 31 431 4.95e-117 370
MsaG024101 MtrunA17_Chr2g0285461 52.459 366 157 5 55 404 19 383 1.86e-116 375
MsaG024101 MtrunA17_Chr2g0285461 46.667 90 46 2 697 785 775 863 5.45e-17 85.9
MsaG024101 MtrunA17_Chr2g0282961 46.683 407 184 4 53 437 47 442 5.13e-115 362
MsaG024101 MtrunA17_Chr8g0376771 50.685 365 164 4 55 404 19 382 3.89e-113 366
MsaG024101 MtrunA17_Chr8g0376771 43.434 99 54 2 697 794 781 878 1.31e-16 84.7
MsaG024101 MtrunA17_Chr5g0433301 50.970 361 159 4 55 397 20 380 8.37e-113 363
MsaG024101 MtrunA17_Chr5g0433301 44.828 87 46 2 700 785 718 803 1.46e-15 81.3
MsaG024101 MtrunA17_Chr3g0109391 50.820 366 156 6 53 397 20 382 3.21e-112 362
MsaG024101 MtrunA17_Chr3g0109391 47.126 87 44 2 700 785 726 811 4.98e-16 82.8
MsaG024101 MtrunA17_Chr1g0156321 52.874 348 130 6 55 378 39 376 4.07e-109 354
MsaG024101 MtrunA17_Chr1g0156321 42.857 91 50 2 697 786 754 843 3.61e-15 80.1
MsaG024101 MtrunA17_Chr4g0070181 50.000 378 174 3 47 410 11 387 7.03e-107 353
MsaG024101 MtrunA17_Chr4g0070181 42.105 133 72 4 692 820 989 1120 1.44e-21 100
MsaG024101 MtrunA17_Chr2g0301251 51.282 351 156 3 47 383 11 360 6.66e-103 343
MsaG024101 MtrunA17_Chr2g0301251 37.313 134 78 4 692 820 981 1113 8.99e-18 88.6
MsaG024101 MtrunA17_Chr8g0389081 49.857 349 152 5 53 382 26 370 1.85e-98 330
MsaG024101 MtrunA17_Chr8g0389081 43.860 114 59 4 697 806 985 1097 2.27e-19 94.0
MsaG024101 MtrunA17_Chr2g0326761 38.861 404 174 9 52 395 4 394 1.32e-73 254
MsaG024101 MtrunA17_Chr4g0028971 38.000 400 175 9 59 396 12 400 5.93e-69 241
MsaG024101 MtrunA17_Chr1g0199681 37.226 411 185 10 47 398 82 478 1.17e-68 240
MsaG024101 MtrunA17_Chr1g0181211 34.293 417 211 7 38 396 6 417 2.35e-66 234
MsaG024101 MtrunA17_Chr8g0356211 46.058 241 114 5 170 396 2 240 8.50e-64 216
MsaG024101 MtrunA17_Chr7g0264171 35.323 402 188 11 59 395 31 425 1.48e-58 210
MsaG024101 MtrunA17_Chr3g0115271 33.488 430 221 8 54 436 14 425 3.93e-57 206
MsaG024101 MtrunA17_Chr7g0264161 38.585 311 172 8 68 370 1 300 5.67e-55 192
MsaG024101 MtrunA17_Chr5g0424931 32.181 376 211 9 59 398 19 386 6.70e-51 187
MsaG024101 MtrunA17_Chr5g0417281 30.935 417 234 10 58 446 10 400 1.78e-50 186
MsaG024101 MtrunA17_Chr1g0178011 31.180 356 225 5 59 400 12 361 4.56e-47 174
MsaG024101 MtrunA17_Chr2g0277461 32.394 355 214 6 59 399 13 355 7.25e-44 165
MsaG024101 MtrunA17_Chr5g0436761 32.597 362 206 10 58 398 11 355 7.83e-44 165
MsaG024101 MtrunA17_Chr2g0289221 33.435 329 195 9 59 381 20 330 3.35e-43 160
MsaG024101 MtrunA17_Chr5g0417211 31.804 327 183 9 59 370 12 313 5.28e-43 161
MsaG024101 MtrunA17_Chr5g0425661 38.776 196 109 3 59 250 37 225 2.50e-37 140
MsaG024101 MtrunA17_Chr2g0277531 28.972 321 196 9 59 370 20 317 2.01e-35 137
MsaG024101 MtrunA17_Chr5g0424801 28.280 343 204 9 59 378 12 335 1.85e-33 132
MsaG024101 MtrunA17_Chr5g0425261 27.988 343 205 9 59 378 12 335 3.50e-31 126
MsaG024101 MtrunA17_Chr5g0424741 27.320 388 214 13 59 392 12 385 5.70e-30 125
MsaG024101 MtrunA17_Chr2g0330951 30.769 117 58 2 700 793 150 266 2.93e-12 68.2
MsaG024101 MtrunA17_Chr1g0156301 37.778 90 40 2 697 786 177 250 9.17e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024101 AT5G62000.2 62.844 872 240 14 13 818 1 854 0.0 1048
MsaG024101 AT5G62000.5 62.844 872 240 14 13 818 1 854 0.0 1048
MsaG024101 AT5G62000.3 62.844 872 240 14 13 818 1 854 0.0 1048
MsaG024101 AT5G62000.1 62.844 872 240 14 13 818 1 854 0.0 1048
MsaG024101 AT5G62000.4 63.164 866 233 15 13 812 1 846 0.0 1041
MsaG024101 AT1G59750.4 66.575 365 106 3 55 404 18 381 1.86e-169 505
MsaG024101 AT1G59750.4 36.667 210 120 4 578 787 431 627 2.55e-32 134
MsaG024101 AT1G59750.2 66.575 365 106 3 55 404 18 381 2.77e-169 505
MsaG024101 AT1G59750.2 36.667 210 120 4 578 787 433 629 2.47e-32 134
MsaG024101 AT1G59750.1 64.829 381 118 3 39 404 2 381 3.43e-169 505
MsaG024101 AT1G59750.1 36.667 210 120 4 578 787 436 632 2.49e-32 134
MsaG024101 AT1G59750.3 64.829 381 118 3 39 404 2 381 3.43e-169 505
MsaG024101 AT1G59750.3 36.667 210 120 4 578 787 436 632 2.49e-32 134
MsaG024101 AT3G61830.1 60.630 381 128 7 37 402 6 379 1.02e-149 452
MsaG024101 AT3G61830.1 50.000 118 56 2 695 812 487 601 5.21e-29 123
MsaG024101 AT2G46530.1 60.989 364 123 6 41 390 3 361 6.11e-149 450
MsaG024101 AT2G46530.1 38.249 217 90 6 577 790 407 582 2.10e-29 124
MsaG024101 AT2G46530.3 63.037 349 110 6 56 390 39 382 3.37e-148 449
MsaG024101 AT2G46530.3 38.249 217 90 6 577 790 428 603 3.16e-29 124
MsaG024101 AT4G23980.1 58.115 382 143 4 56 423 9 387 9.67e-143 436
MsaG024101 AT4G23980.1 41.709 199 91 6 593 788 437 613 4.46e-32 133
MsaG024101 AT4G23980.2 58.839 379 132 5 56 413 9 384 2.44e-142 434
MsaG024101 AT4G23980.2 41.709 199 91 6 593 788 435 611 5.04e-32 133
MsaG024101 AT3G61830.2 60.542 332 112 6 86 402 1 328 1.87e-128 395
MsaG024101 AT3G61830.2 50.000 118 56 2 695 812 436 550 3.19e-29 124
MsaG024101 AT5G60450.2 48.039 408 184 5 54 438 36 438 3.31e-126 397
MsaG024101 AT5G60450.2 53.191 94 44 0 690 783 634 727 5.26e-28 121
MsaG024101 AT5G60450.1 48.276 406 182 5 56 438 62 462 1.37e-125 395
MsaG024101 AT5G60450.1 53.191 94 44 0 690 783 658 751 6.24e-28 121
MsaG024101 AT5G37020.2 50.794 378 163 5 49 407 12 385 6.11e-115 367
MsaG024101 AT5G37020.2 43.243 74 40 2 693 765 701 773 1.31e-11 68.9
MsaG024101 AT5G37020.1 50.794 378 163 5 49 407 12 385 1.55e-114 367
MsaG024101 AT5G37020.1 40.426 94 54 2 693 785 701 793 7.42e-15 79.3
MsaG024101 AT1G30330.1 52.459 366 157 5 55 404 19 383 3.13e-114 370
MsaG024101 AT1G30330.1 33.898 177 103 6 611 785 718 882 1.63e-16 84.7
MsaG024101 AT1G30330.3 52.459 366 157 5 55 404 21 385 3.70e-114 369
MsaG024101 AT1G30330.3 33.898 177 103 6 611 785 720 884 1.57e-16 85.1
MsaG024101 AT1G30330.2 52.459 366 157 5 55 404 21 385 3.70e-114 369
MsaG024101 AT1G30330.2 33.898 177 103 6 611 785 720 884 1.57e-16 85.1
MsaG024101 AT1G35540.1 50.000 346 141 5 52 378 20 352 2.42e-113 358
MsaG024101 AT1G35540.1 50.000 102 49 2 692 792 505 605 2.34e-25 112
MsaG024101 AT1G34310.1 48.787 371 154 7 36 387 6 359 1.27e-112 356
MsaG024101 AT1G34310.1 50.588 85 41 1 692 776 506 589 6.74e-22 101
MsaG024101 AT2G33860.1 45.833 408 188 5 52 437 47 443 1.54e-112 356
MsaG024101 AT1G35240.1 48.219 365 165 6 36 387 6 359 5.73e-112 354
MsaG024101 AT1G35240.1 52.174 92 43 1 692 783 492 582 5.86e-25 110
MsaG024101 AT1G34410.1 44.686 414 181 9 36 428 6 392 1.88e-111 353
MsaG024101 AT1G34410.1 51.087 92 44 1 692 783 506 596 3.28e-24 108
MsaG024101 AT1G35520.1 47.709 371 159 7 37 387 4 359 1.72e-110 350
MsaG024101 AT1G35520.1 32.474 194 95 5 584 776 431 589 1.83e-22 103
MsaG024101 AT1G19850.1 52.478 343 132 5 59 378 54 388 2.49e-108 353
MsaG024101 AT1G19850.1 46.739 92 47 2 696 786 792 882 5.02e-18 89.7
MsaG024101 AT1G19850.2 52.478 343 132 5 59 378 43 377 3.09e-108 353
MsaG024101 AT1G19850.2 46.739 92 47 2 696 786 781 871 5.24e-18 89.7
MsaG024101 AT1G34390.1 50.000 362 142 8 52 394 20 361 5.06e-108 344
MsaG024101 AT1G34390.1 47.727 88 45 1 692 779 504 590 4.66e-21 98.6
MsaG024101 AT1G34170.1 47.633 338 155 5 56 378 22 352 9.34e-107 337
MsaG024101 AT2G46530.2 62.992 254 77 4 150 390 25 274 1.11e-104 332
MsaG024101 AT2G46530.2 36.564 227 80 6 577 790 320 495 8.08e-30 125
MsaG024101 AT5G20730.2 51.515 363 158 4 59 407 24 382 8.81e-104 346
MsaG024101 AT5G20730.2 40.517 116 67 2 692 806 1032 1146 2.48e-19 94.4
MsaG024101 AT1G19220.1 50.689 363 161 4 59 407 23 381 3.16e-103 343
MsaG024101 AT1G19220.1 44.538 119 61 4 692 806 953 1070 8.08e-21 99.0
MsaG024101 AT5G20730.1 51.374 364 158 5 59 407 24 383 4.44e-102 342
MsaG024101 AT5G20730.1 40.517 116 67 2 692 806 1033 1147 2.52e-19 94.4
MsaG024101 AT5G20730.3 51.374 364 158 5 59 407 24 383 4.63e-102 341
MsaG024101 AT5G20730.3 44.792 96 51 2 692 786 1033 1127 5.36e-19 93.2
MsaG024101 AT1G34170.2 44.970 338 138 5 56 378 22 326 6.93e-92 297
MsaG024101 AT1G34170.3 44.970 338 138 5 56 378 22 326 7.98e-91 297
MsaG024101 AT1G34170.3 42.623 122 66 2 667 785 400 520 1.67e-22 102
MsaG024101 AT2G33860.2 48.443 289 132 2 162 437 2 286 5.51e-83 273
MsaG024101 AT5G37020.3 54.231 260 105 2 162 407 31 290 8.23e-81 275
MsaG024101 AT5G37020.3 40.426 94 54 2 693 785 606 698 6.87e-15 79.3
MsaG024101 AT2G28350.2 38.119 404 185 8 51 395 2 399 1.33e-73 254
MsaG024101 AT2G28350.1 38.119 404 185 8 51 395 2 399 1.90e-73 254
MsaG024101 AT4G30080.1 36.993 419 187 13 53 411 13 414 8.80e-70 243
MsaG024101 AT1G43950.1 50.000 226 105 4 37 261 4 222 2.40e-65 218
MsaG024101 AT1G77850.2 32.308 390 196 7 60 396 20 394 2.18e-48 181
MsaG024101 AT1G77850.1 32.308 390 196 7 60 396 20 394 2.34e-48 181
MsaG024101 AT5G25890.1 31.818 88 59 1 700 787 83 169 5.50e-12 65.5
MsaG024101 AT3G16500.1 30.709 127 67 3 673 782 131 253 4.16e-11 65.1
MsaG024101 AT3G16500.2 30.709 127 67 3 673 782 131 253 4.57e-11 64.7
MsaG024101 AT1G04550.2 28.462 130 79 3 683 799 109 237 8.16e-11 63.5

Find 195 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATCAGCTATTTGAATT+TGG 0.210673 4:+95806071 MsaT024101.1:CDS
ATCGACCTTGAGAGGAAATT+TGG 0.250119 4:+95805128 MsaT024101.1:CDS
TTTAGATGTTGGCCATCTTT+TGG 0.254225 4:-95804751 None:intergenic
GGGATGCATTTATATTTCTA+AGG 0.282680 4:+95804075 MsaT024101.1:CDS
TTCCAATGCCCAGGCCTAAA+AGG 0.287769 4:+95804883 MsaT024101.1:CDS
TTTCATGAAGAGTGAATGTT+TGG 0.299494 4:+95802533 None:intergenic
AAAGATGGCCAACATCTAAA+TGG 0.327483 4:+95804754 MsaT024101.1:CDS
GACAGACCAGGTGGCATTAC+AGG 0.333510 4:-95805357 None:intergenic
AGTTCTAAGAGGCTGGTTGC+TGG 0.335910 4:+95804053 MsaT024101.1:CDS
GGTGATTACTCAGTGCTTCA+TGG 0.355709 4:+95805474 MsaT024101.1:CDS
TGATGAATTAACCGCTGAAT+TGG 0.360324 4:+95806052 MsaT024101.1:CDS
AGCTTACAGTCACTGTCTTT+CGG 0.367081 4:-95805624 None:intergenic
TCGGACCTTCTGTCGGGATT+TGG 0.372145 4:+95805294 MsaT024101.1:CDS
CGGGTAAGAACAGAAGAATC+TGG 0.391132 4:-95804933 None:intergenic
CTCATTCCCGTGCAGATCCT+TGG 0.394358 4:-95803850 None:intergenic
GAAGGTATGGTTCAGAGAAC+TGG 0.394883 4:+95805241 MsaT024101.1:CDS
GCATGTTATGTTTGCCTTCA+CGG 0.404339 4:-95805323 None:intergenic
GCAAACACCTCATCAGTATC+TGG 0.405128 4:-95803391 None:intergenic
ATTGAAGGGTCCGGCATAGT+TGG 0.409564 4:-95805672 None:intergenic
CTTCTAGCAGAACCACTTTG+AGG 0.409668 4:-95805885 None:intergenic
CTGTGACGATTCTGTAGAAT+GGG 0.411759 4:-95805565 None:intergenic
CTTAGGACGGAGATCGTAAA+CGG 0.416054 4:-95803172 None:intergenic
TGATGAATGCCTTCCTCCAC+TGG 0.419523 4:+95803699 MsaT024101.1:CDS
GGTTCTTCATCGTTATCAAC+AGG 0.419682 4:+95802671 MsaT024101.1:CDS
CTTTGAGAAATTGAAGGGTC+CGG 0.420099 4:-95805681 None:intergenic
AATATGAGATACAGTGCATT+TGG 0.421814 4:+95805453 MsaT024101.1:CDS
CATGTTATGTTTGCCTTCAC+GGG 0.422513 4:-95805322 None:intergenic
GTTCTAAGAGGCTGGTTGCT+GGG 0.423320 4:+95804054 MsaT024101.1:CDS
CTTGACCTTGCAAGACCCTC+TGG 0.423502 4:-95805101 None:intergenic
ACAACCACCTACACAGGAAT+TGG 0.425208 4:+95803824 MsaT024101.1:CDS
AACACAAGTTCTCCTTCTCT+TGG 0.429337 4:-95802856 None:intergenic
GACAGTGACTGTAAGCTATT+CGG 0.430523 4:+95805630 MsaT024101.1:CDS
ATGACCCGACAAAGGATCTT+AGG 0.431740 4:-95803189 None:intergenic
CGGTTTAAAATGAGGTTTGA+GGG 0.432278 4:+95804543 MsaT024101.1:CDS
CTGCACTTTACAGAGAACTA+TGG 0.437647 4:+95802803 MsaT024101.1:CDS
AAGGCTGGAACCAACGTATT+CGG 0.439474 4:+95805275 MsaT024101.1:CDS
TTATTTAAGGTTCACAAGAA+AGG 0.439534 4:+95805984 MsaT024101.1:intron
ACGTATTCGGACCTTCTGTC+GGG 0.452204 4:+95805288 MsaT024101.1:CDS
TTGAAGGGTCCGGCATAGTT+GGG 0.454051 4:-95805671 None:intergenic
TGAAATCGCGGCGGAGGAGC+AGG 0.455495 4:-95803598 None:intergenic
TCGGTGTTCTTGCGACTGCT+TGG 0.455889 4:+95804276 MsaT024101.1:CDS
TATTTAAGGTTCACAAGAAA+GGG 0.456345 4:+95805985 MsaT024101.1:intron
GCAGGGTAATGTTCCATCTT+CGG 0.458519 4:+95804229 MsaT024101.1:CDS
GGAAATTCTGTCAACGGAAA+AGG 0.460893 4:+95802611 MsaT024101.1:CDS
TCCACAAGGACATATTGAAC+AGG 0.462225 4:+95802885 MsaT024101.1:CDS
GATCATTCATACCTTTGCTC+AGG 0.462655 4:-95804576 None:intergenic
TCCGTCCTAAGATCCTTTGT+CGG 0.466899 4:+95803184 MsaT024101.1:CDS
GTTTCTACTTCAAGAAGGTA+TGG 0.468821 4:+95805228 MsaT024101.1:CDS
GGGAGCTTCGAGTTGGTGTC+AGG 0.469625 4:+95804192 MsaT024101.1:CDS
TTCAGCCAAATTTCCTCTCA+AGG 0.477911 4:-95805133 None:intergenic
AATGTTTGGGATGAGAGATC+TGG 0.480947 4:+95802547 None:intergenic
TTCTAAGAGGCTGGTTGCTG+GGG 0.482649 4:+95804055 MsaT024101.1:CDS
GCATGCGTGTGCTGGTCCTT+TGG 0.485332 4:+95802825 MsaT024101.1:CDS
TTAGGCCTGGGCATTGGAAG+AGG 0.486254 4:-95804879 None:intergenic
CACAAGAAAGGGATTGCGCT+TGG 0.489727 4:+95805996 MsaT024101.1:CDS
ATCTCATATTACCTTGAGCT+TGG 0.492191 4:-95805440 None:intergenic
AGACATCTCCATTTAGATGT+TGG 0.493538 4:-95804762 None:intergenic
TCTGTGACGATTCTGTAGAA+TGG 0.496331 4:-95805566 None:intergenic
CGTGGTCTTCACTGATAATG+AGG 0.499290 4:+95806127 MsaT024101.1:CDS
TGAACTAATATCTCCACAAA+AGG 0.500571 4:+95806097 MsaT024101.1:CDS
AACGTATTCGGACCTTCTGT+CGG 0.500644 4:+95805287 MsaT024101.1:CDS
TAATATCTCCACAAAAGGAT+TGG 0.501274 4:+95806102 MsaT024101.1:CDS
AGGTTTACAGGTACCATTGT+TGG 0.502878 4:+95804711 MsaT024101.1:intron
GACATTTGCTGCAGAGTGGT+TGG 0.502895 4:+95804018 MsaT024101.1:CDS
TAGGCATGCGGTTTAAAATG+AGG 0.504222 4:+95804535 MsaT024101.1:CDS
TTCTTGCACTCTAATATGAA+AGG 0.507078 4:+95805387 MsaT024101.1:CDS
GATCAGCTATTTGAATTTGG+AGG 0.508832 4:+95806074 MsaT024101.1:CDS
ATCATCAGAAGTATCTATGA+AGG 0.509774 4:+95802589 MsaT024101.1:CDS
AATCGCGGCGGAGGAGCAGG+TGG 0.515442 4:-95803595 None:intergenic
AACATAACATGCTTACTCAA+TGG 0.515530 4:+95805334 MsaT024101.1:CDS
GGAAACCACTAGTGGGCAAA+GGG 0.516386 4:-95805080 None:intergenic
TCGTATTGAGTTGGTGGTGG+TGG 0.520513 4:-95805537 None:intergenic
GCTGTTGGAGATGCTCAAAA+TGG 0.520529 4:+95802650 MsaT024101.1:CDS
CTGGATGTCAATGTCAAGGC+TGG 0.520967 4:+95805260 MsaT024101.1:CDS
CCGTCCTAAGATCCTTTGTC+GGG 0.521751 4:+95803185 MsaT024101.1:CDS
TTCTTGTGCATCTCCGCCAT+CGG 0.523242 4:-95806395 None:intergenic
ACATTTCTTTGAGAAATTGA+AGG 0.523337 4:-95805687 None:intergenic
CAGGCGTGCAATGAGACAGC+AGG 0.524850 4:+95804211 MsaT024101.1:CDS
TCATCGTTATCAACAGGAAG+AGG 0.527310 4:+95802677 MsaT024101.1:CDS
GAGGGTGACATGATGCTTGT+TGG 0.529132 4:+95806146 MsaT024101.1:CDS
GACTCCATGTATTGATCATA+CGG 0.529240 4:-95804492 None:intergenic
TGCAACTGATGAAGAAAAGA+TGG 0.529963 4:+95805200 MsaT024101.1:CDS
CTCTGGTACCAAAGTCACTT+GGG 0.530252 4:-95803413 None:intergenic
TGCTTGTTGGCGACGATCCA+TGG 0.532108 4:+95806159 MsaT024101.1:CDS
AAGTTCTCATGATACCTTCA+CGG 0.533026 4:-95804953 None:intergenic
CTCTCGTATTGAGTTGGTGG+TGG 0.533181 4:-95805540 None:intergenic
GGACTCTCGTATTGAGTTGG+TGG 0.533575 4:-95805543 None:intergenic
GGGTAAGAACAGAAGAATCT+GGG 0.533836 4:-95804932 None:intergenic
TCTCAGTAGCCCAACTATGC+CGG 0.534348 4:+95805662 MsaT024101.1:CDS
GAACTATGGCATGCGTGTGC+TGG 0.534486 4:+95802817 MsaT024101.1:CDS
ACTATGACTAGTTTATCTGC+TGG 0.535958 4:-95805801 None:intergenic
TGAACATGAAATCGCGGCGG+AGG 0.536929 4:-95803604 None:intergenic
GTCCTGAGAGAGTTTCCCCT+TGG 0.537173 4:+95804820 MsaT024101.1:intron
TATTGAGTTGGTGGTGGTGG+TGG 0.537370 4:-95805534 None:intergenic
TAAATCCTCTTCCAATGCCC+AGG 0.538984 4:+95804874 MsaT024101.1:CDS
TTGGCCACCAATTCCTGTGT+AGG 0.539634 4:-95803831 None:intergenic
GCCCACTAGTGGTTTCCAGA+GGG 0.539744 4:+95805086 MsaT024101.1:CDS
TTGGTAACGGTTCCAAGAGA+AGG 0.539960 4:+95802844 MsaT024101.1:CDS
TCTTCTGTTCTTACCCGTGA+AGG 0.542731 4:+95804939 MsaT024101.1:CDS
AGGGATTGCGCTTGGTAGGT+CGG 0.543005 4:+95806004 MsaT024101.1:CDS
GGTGTTTGCCCAAGTGACTT+TGG 0.543173 4:+95803405 MsaT024101.1:CDS
GGTCATCAACGTTATGCTAA+AGG 0.544071 4:+95803206 MsaT024101.1:CDS
TCGCGGCGGAGGAGCAGGTG+GGG 0.544587 4:-95803593 None:intergenic
ATGGACCCTTTGCCCACTAG+TGG 0.547335 4:+95805075 MsaT024101.1:CDS
AGAAAGGGATTGCGCTTGGT+AGG 0.549488 4:+95806000 MsaT024101.1:CDS
GCTCTGGTACCAAAGTCACT+TGG 0.550915 4:-95803414 None:intergenic
TGGCTAGAGATAACCGAAGA+TGG 0.550920 4:-95804242 None:intergenic
ACCACCTACACAGGAATTGG+TGG 0.550997 4:+95803827 MsaT024101.1:CDS
TACGGAACAATAAACTCCGC+TGG 0.551013 4:-95804474 None:intergenic
CATTTCTTTGAGAAATTGAA+GGG 0.551673 4:-95805686 None:intergenic
TTAGGACGGAGATCGTAAAC+GGG 0.552349 4:-95803171 None:intergenic
GCATCTGATACTAGTACTCA+TGG 0.552448 4:+95803649 MsaT024101.1:CDS
GCGGTTTAAAATGAGGTTTG+AGG 0.557240 4:+95804542 MsaT024101.1:CDS
AATGGTGATACTTACTGCCA+TGG 0.558365 4:-95806176 None:intergenic
AGAGCTGGAGGAGCAAGAGC+AGG 0.558684 4:-95804852 None:intergenic
TTCATGAAGAGTGAATGTTT+GGG 0.559177 4:+95802534 None:intergenic
TGCCCACTAGTGGTTTCCAG+AGG 0.560131 4:+95805085 MsaT024101.1:CDS
AAGACTCTGACTCCAAAAGA+TGG 0.560145 4:+95804739 MsaT024101.1:CDS
CAAGAATCATCGACCTTGAG+AGG 0.560987 4:+95805120 MsaT024101.1:CDS
GCCACCAATTCCTGTGTAGG+TGG 0.565859 4:-95803828 None:intergenic
AATGAGGTTTGAGGGTGAAG+AGG 0.565915 4:+95804551 MsaT024101.1:CDS
ATGCTGTTTCTACTTCAAGA+AGG 0.568887 4:+95805223 MsaT024101.1:CDS
TACACAGGAATTGGTGGCCA+AGG 0.568988 4:+95803833 MsaT024101.1:CDS
CAATGGCCTGTAATGCCACC+TGG 0.569696 4:+95805351 MsaT024101.1:CDS
TGGAAACCACTAGTGGGCAA+AGG 0.571230 4:-95805081 None:intergenic
AGCATCGTCAAAAGTATGCA+TGG 0.573669 4:+95805056 MsaT024101.1:intron
ATGAAGGGAAATTCTGTCAA+CGG 0.574818 4:+95802605 MsaT024101.1:CDS
ATCGCGGCGGAGGAGCAGGT+GGG 0.579243 4:-95803594 None:intergenic
AGGATCTGCACGGGAATGAG+TGG 0.579697 4:+95803853 MsaT024101.1:CDS
GTGTGCTGGTCCTTTGGTAA+CGG 0.580243 4:+95802831 MsaT024101.1:CDS
GAGAACAAACCAGTGGAGGA+AGG 0.582901 4:-95803708 None:intergenic
AGCAGAGAACAAACCAGTGG+AGG 0.583434 4:-95803712 None:intergenic
GAACACCGAGATGCATGCTA+TGG 0.587167 4:-95804262 None:intergenic
TGGGGACTCTCGTATTGAGT+TGG 0.588356 4:-95805546 None:intergenic
TGTTCCGTATGATCAATACA+TGG 0.589206 4:+95804488 MsaT024101.1:CDS
GTCAAAACAACCACCTACAC+AGG 0.589887 4:+95803818 MsaT024101.1:CDS
GGAAGAGGATTTAGAGCTGG+AGG 0.589947 4:-95804864 None:intergenic
GAAAGCAACGTTGATGCTGT+TGG 0.591943 4:+95802635 MsaT024101.1:CDS
ATTGGAAGAGGATTTAGAGC+TGG 0.592035 4:-95804867 None:intergenic
ATCTAAATGGAGATGTCTCA+AGG 0.592490 4:+95804767 MsaT024101.1:CDS
GACCCTCTGGAAACCACTAG+TGG 0.593537 4:-95805088 None:intergenic
TGGTGGCCAAGGATCTGCAC+GGG 0.594504 4:+95803844 MsaT024101.1:CDS
AGTTCTCATGATACCTTCAC+GGG 0.595152 4:-95804952 None:intergenic
AAGAAATTTAGCGACAGACC+AGG 0.596232 4:-95805369 None:intergenic
ACAGAAGAATCTGGGGAGGA+CGG 0.596550 4:-95804924 None:intergenic
AGTTGAACATCTTTAACATG+AGG 0.596830 4:-95805858 None:intergenic
AGCTTCGACAAATCAAGCAT+CGG 0.597524 4:+95803137 MsaT024101.1:CDS
ATGTTGTGATGAAATTTGCA+TGG 0.600259 4:-95805730 None:intergenic
TCACTGTTTATTACAAACCC+CGG 0.603096 4:+95804324 MsaT024101.1:CDS
AATGCAACTTACCAAGCTCA+AGG 0.604596 4:+95805429 MsaT024101.1:CDS
CACTACCAAATCCCGACAGA+AGG 0.606193 4:-95805299 None:intergenic
AATATGAAAGGTTCTGCACA+AGG 0.609450 4:+95805399 MsaT024101.1:CDS
TCTAGCCATAGCATGCATCT+CGG 0.612124 4:+95804257 MsaT024101.1:CDS
GTGAAGAGGCTCCTGAGCAA+AGG 0.613106 4:+95804565 MsaT024101.1:CDS
GAGATTCAGAAAATGAGCCC+AGG 0.615563 4:+95806334 MsaT024101.1:CDS
GAGAATGGGGAGCTTCGAGT+TGG 0.619668 4:+95804185 MsaT024101.1:CDS
TCTCTTGGAACCGTTACCAA+AGG 0.623814 4:-95802841 None:intergenic
AGAACTGGATGTCAATGTCA+AGG 0.624645 4:+95805256 MsaT024101.1:CDS
GGTTTCCAGAGGGTCTTGCA+AGG 0.624805 4:+95805096 MsaT024101.1:CDS
TCATCAGAAGTATCTATGAA+GGG 0.626313 4:+95802590 MsaT024101.1:CDS
GTTCAACTCAAACCTCAAAG+TGG 0.626662 4:+95805873 MsaT024101.1:CDS
TAAAAGGCCTCGAGCTAACG+TGG 0.628300 4:+95804899 MsaT024101.1:CDS
CTGCAGAGAAGTCCGTTGCG+TGG 0.628798 4:+95805172 MsaT024101.1:CDS
ACCTGTTCAATATGTCCTTG+TGG 0.632841 4:-95802886 None:intergenic
AGTTGCAGGCGTCCACGCAA+CGG 0.636265 4:-95805184 None:intergenic
CTTCTGCAGGACAAGTCCAG+CGG 0.639810 4:+95804458 MsaT024101.1:intron
GGATTTGGTAGTGCCCGTGA+AGG 0.642628 4:+95805309 MsaT024101.1:CDS
CGTAGACATTTGCTGCAGAG+TGG 0.643093 4:+95804014 MsaT024101.1:CDS
CCCGACAAAGGATCTTAGGA+CGG 0.643609 4:-95803185 None:intergenic
TGTTGTGATGAAATTTGCAT+GGG 0.646081 4:-95805729 None:intergenic
TTTGTAATAAACAGTGAACA+TGG 0.646135 4:-95804318 None:intergenic
GACGGCACCACGTTAGCTCG+AGG 0.646861 4:-95804906 None:intergenic
ACCCTCTGGAAACCACTAGT+GGG 0.647981 4:-95805087 None:intergenic
AATCATTCAGCTACCGATGG+CGG 0.649175 4:+95806382 MsaT024101.1:CDS
TCCACAAAAGGATTGGCTCG+TGG 0.649190 4:+95806109 MsaT024101.1:CDS
GAGAATCATTCAGCTACCGA+TGG 0.649809 4:+95806379 MsaT024101.1:CDS
AGAGTCTTCAATGCCAACAA+TGG 0.650034 4:-95804724 None:intergenic
AACGAATGAACATGAAATCG+CGG 0.650113 4:-95803610 None:intergenic
GTGGTCTTCACTGATAATGA+GGG 0.652302 4:+95806128 MsaT024101.1:CDS
CTCTGCAGCAAATGTCTACG+TGG 0.656550 4:-95804011 None:intergenic
AGGCGTGCAATGAGACAGCA+GGG 0.658437 4:+95804212 MsaT024101.1:CDS
TCTGGAAGAAGATAGAAACA+TGG 0.661616 4:+95802565 MsaT024101.1:CDS
ATAATTATACAATAGGCATG+CGG 0.665775 4:+95804523 MsaT024101.1:CDS
TAACGTTGATGACCCGACAA+AGG 0.669433 4:-95803197 None:intergenic
CATAAAGTTGAAAATCCACA+CGG 0.673033 4:+95805498 MsaT024101.1:CDS
TGTGACGATTCTGTAGAATG+GGG 0.680247 4:-95805564 None:intergenic
TTGGTGGCCAAGGATCTGCA+CGG 0.683444 4:+95803843 MsaT024101.1:CDS
AAATAGCTGATCCAATTCAG+CGG 0.687488 4:-95806063 None:intergenic
GGCGGAGCCAGATACTGATG+AGG 0.688401 4:+95803384 MsaT024101.1:intron
AAGAACAGAAGAATCTGGGG+AGG 0.702793 4:-95804928 None:intergenic
AAATTTAGCGACAGACCAGG+TGG 0.706484 4:-95805366 None:intergenic
GACAGAAGGTCCGAATACGT+TGG 0.706729 4:-95805285 None:intergenic
GGTAAGAACAGAAGAATCTG+GGG 0.709548 4:-95804931 None:intergenic
TCTGATACTAGTACTCATGG+TGG 0.760741 4:+95803652 MsaT024101.1:CDS
GAATGAACATGAAATCGCGG+CGG 0.768456 4:-95803607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 95802548 95806456 95802548 ID=MsaG024101
Chr4 mRNA 95802548 95806456 95802548 ID=MsaT024101.1;Parent=MsaG024101
Chr4 exon 95802548 95802698 95802548 ID=MsaT024101.1.exon1;Parent=MsaT024101.1
Chr4 CDS 95802548 95802698 95802548 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95802794 95802906 95802794 ID=MsaT024101.1.exon2;Parent=MsaT024101.1
Chr4 CDS 95802794 95802906 95802794 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95803132 95803227 95803132 ID=MsaT024101.1.exon3;Parent=MsaT024101.1
Chr4 CDS 95803132 95803227 95803132 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95803385 95803441 95803385 ID=MsaT024101.1.exon4;Parent=MsaT024101.1
Chr4 CDS 95803385 95803441 95803385 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95803565 95803720 95803565 ID=MsaT024101.1.exon5;Parent=MsaT024101.1
Chr4 CDS 95803565 95803720 95803565 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95803813 95803897 95803813 ID=MsaT024101.1.exon6;Parent=MsaT024101.1
Chr4 CDS 95803813 95803897 95803813 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95804006 95804096 95804006 ID=MsaT024101.1.exon7;Parent=MsaT024101.1
Chr4 CDS 95804006 95804096 95804006 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95804181 95804345 95804181 ID=MsaT024101.1.exon8;Parent=MsaT024101.1
Chr4 CDS 95804181 95804345 95804181 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95804467 95804586 95804467 ID=MsaT024101.1.exon9;Parent=MsaT024101.1
Chr4 CDS 95804467 95804586 95804467 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95804713 95804788 95804713 ID=MsaT024101.1.exon10;Parent=MsaT024101.1
Chr4 CDS 95804713 95804788 95804713 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95804828 95804960 95804828 ID=MsaT024101.1.exon11;Parent=MsaT024101.1
Chr4 CDS 95804828 95804960 95804828 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95805058 95805917 95805058 ID=MsaT024101.1.exon12;Parent=MsaT024101.1
Chr4 CDS 95805058 95805917 95805058 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95805993 95806183 95805993 ID=MsaT024101.1.exon13;Parent=MsaT024101.1
Chr4 CDS 95805993 95806183 95805993 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Chr4 exon 95806288 95806456 95806288 ID=MsaT024101.1.exon14;Parent=MsaT024101.1
Chr4 CDS 95806288 95806456 95806288 ID=cds.MsaT024101.1;Parent=MsaT024101.1
Gene Sequence

>MsaT024101.1

ATGTTTGGGATGAGAGATCTGGAAGAAGATAGAAACATGGCATCATCAGAAGTATCTATGAAGGGAAATTCTGTCAACGGAAAAGGTGAAAGCAACGTTGATGCTGTTGGAGATGCTCAAAATGGTTCTTCATCGTTATCAACAGGAAGAGAAGCTGAAGCTGCACTTTACAGAGAACTATGGCATGCGTGTGCTGGTCCTTTGGTAACGGTTCCAAGAGAAGGAGAACTTGTGTTCTATTTTCCACAAGGACATATTGAACAGGTGGAAGCTTCGACAAATCAAGCATCGGAACAACACATGCCCGTTTACGATCTCCGTCCTAAGATCCTTTGTCGGGTCATCAACGTTATGCTAAAGGCGGAGCCAGATACTGATGAGGTGTTTGCCCAAGTGACTTTGGTACCAGAGCCCAATCAAGATGAAAATGCTGTTGAGAAAGAAGCCCCACCTGCTCCTCCGCCGCGATTTCATGTTCATTCGTTTTGTAAAACATTGACTGCATCTGATACTAGTACTCATGGTGGTTTTTCTGTACTTAGACGTCATGCTGATGAATGCCTTCCTCCACTGGATATGTCAAAACAACCACCTACACAGGAATTGGTGGCCAAGGATCTGCACGGGAATGAGTGGCGTTTTAGGCATATTTTTCGTGGTCAACCACGTAGACATTTGCTGCAGAGTGGTTGGAGTGTTTTTGTCAGTTCTAAGAGGCTGGTTGCTGGGGATGCATTTATATTTCTAAGAGGTGAGAATGGGGAGCTTCGAGTTGGTGTCAGGCGTGCAATGAGACAGCAGGGTAATGTTCCATCTTCGGTTATCTCTAGCCATAGCATGCATCTCGGTGTTCTTGCGACTGCTTGGCATGCTGTTTTGACTGGGACCATGTTCACTGTTTATTACAAACCCCGGACAAGTCCAGCGGAGTTTATTGTTCCGTATGATCAATACATGGAGTCTCTAAAAAATAATTATACAATAGGCATGCGGTTTAAAATGAGGTTTGAGGGTGAAGAGGCTCCTGAGCAAAGGTTTACAGGTACCATTGTTGGCATTGAAGACTCTGACTCCAAAAGATGGCCAACATCTAAATGGAGATGTCTCAAGAGAGTTTCCCCTTGGAAAATAGAGCCTGCTCTTGCTCCTCCAGCTCTAAATCCTCTTCCAATGCCCAGGCCTAAAAGGCCTCGAGCTAACGTGGTGCCGTCCTCCCCAGATTCTTCTGTTCTTACCCGTGAAGCATCGTCAAAAGTATGCATGGACCCTTTGCCCACTAGTGGTTTCCAGAGGGTCTTGCAAGGTCAAGAATCATCGACCTTGAGAGGAAATTTGGCTGAAAGCAACGACTCTTACACTGCAGAGAAGTCCGTTGCGTGGACGCCTGCAACTGATGAAGAAAAGATGGATGCTGTTTCTACTTCAAGAAGGTATGGTTCAGAGAACTGGATGTCAATGTCAAGGCTGGAACCAACGTATTCGGACCTTCTGTCGGGATTTGGTAGTGCCCGTGAAGGCAAACATAACATGCTTACTCAATGGCCTGTAATGCCACCTGGTCTGTCGCTAAATTTCTTGCACTCTAATATGAAAGGTTCTGCACAAGGCAGTGATAATGCAACTTACCAAGCTCAAGGTAATATGAGATACAGTGCATTTGGTGATTACTCAGTGCTTCATGGACATAAAGTTGAAAATCCACACGGAAACTTTTTGATGCCACCACCACCACCAACTCAATACGAGAGTCCCCATTCTACAGAATCGTCACAGAAACAAATGTCTTCAAAAACAAGCGAGGCTGCAAAACCGAAAGACAGTGACTGTAAGCTATTCGGCTTCTCGCTTCTCAGTAGCCCAACTATGCCGGACCCTTCAATTTCTCAAAGAAATGTTACAAACGAGACTTCTAGTCCCATGCAAATTTCATCACAACATCACACATTTGAAAATGATCAGAAGTCTGAACATTCAAAGAGCTTCAAACCAGCAGATAAACTAGTCATAGTTGATGAGCACGAGAAGCAGCTGCAAACTTCTCAGCCTCATGTTAAAGATGTTCAACTCAAACCTCAAAGTGGTTCTGCTAGAAGCTGCACAAAAGTTCACAAGAAAGGGATTGCGCTTGGTAGGTCGGTTGATCTTACAAAGTTTAGTGACTATGATGAATTAACCGCTGAATTGGATCAGCTATTTGAATTTGGAGGTGAACTAATATCTCCACAAAAGGATTGGCTCGTGGTCTTCACTGATAATGAGGGTGACATGATGCTTGTTGGCGACGATCCATGGCAGGAGTTTTGTTCAATGGTTCGCAAAATTTACATCTACCCCAAAGAAGAGATTCAGAAAATGAGCCCAGGCACATTGAGTTCGAAAAATGAAGAGAATCATTCAGCTACCGATGGCGGAGATGCACAAGAAACTAAATCTCAGCTGAATCAATCAGCTTCAGATAATTGA
Protein sequence

>MsaT024101.1

MFGMRDLEEDRNMASSEVSMKGNSVNGKGESNVDAVGDAQNGSSSLSTGREAEAALYRELWHACAGPLVTVPREGELVFYFPQGHIEQVEASTNQASEQHMPVYDLRPKILCRVINVMLKAEPDTDEVFAQVTLVPEPNQDENAVEKEAPPAPPPRFHVHSFCKTLTASDTSTHGGFSVLRRHADECLPPLDMSKQPPTQELVAKDLHGNEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLVAGDAFIFLRGENGELRVGVRRAMRQQGNVPSSVISSHSMHLGVLATAWHAVLTGTMFTVYYKPRTSPAEFIVPYDQYMESLKNNYTIGMRFKMRFEGEEAPEQRFTGTIVGIEDSDSKRWPTSKWRCLKRVSPWKIEPALAPPALNPLPMPRPKRPRANVVPSSPDSSVLTREASSKVCMDPLPTSGFQRVLQGQESSTLRGNLAESNDSYTAEKSVAWTPATDEEKMDAVSTSRRYGSENWMSMSRLEPTYSDLLSGFGSAREGKHNMLTQWPVMPPGLSLNFLHSNMKGSAQGSDNATYQAQGNMRYSAFGDYSVLHGHKVENPHGNFLMPPPPPTQYESPHSTESSQKQMSSKTSEAAKPKDSDCKLFGFSLLSSPTMPDPSISQRNVTNETSSPMQISSQHHTFENDQKSEHSKSFKPADKLVIVDEHEKQLQTSQPHVKDVQLKPQSGSARSCTKVHKKGIALGRSVDLTKFSDYDELTAELDQLFEFGGELISPQKDWLVVFTDNEGDMMLVGDDPWQEFCSMVRKIYIYPKEEIQKMSPGTLSSKNEENHSATDGGDAQETKSQLNQSASDN*