AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG024113

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024113 MtrunA17_Chr8g0385961 99.038 312 3 0 1 312 1 312 0.0 651
MsaG024113 MtrunA17_Chr1g0156031 49.425 348 129 11 3 312 2 340 1.27e-105 311
MsaG024113 MtrunA17_Chr6g0479911 52.336 107 51 0 206 312 183 289 5.57e-43 149
MsaG024113 MtrunA17_Chr8g0375531 53.846 104 48 0 209 312 177 280 1.35e-39 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG024113 AT2G21240.1 58.280 314 111 5 1 312 1 296 3.48e-127 365
MsaG024113 AT2G21240.2 58.280 314 111 5 1 312 1 296 3.48e-127 365
MsaG024113 AT4G38910.2 54.808 312 112 6 1 312 1 283 2.60e-114 332
MsaG024113 AT4G38910.1 56.228 281 96 5 33 312 4 258 4.44e-104 305
MsaG024113 AT5G42520.2 47.899 357 122 11 1 312 1 338 5.63e-91 275
MsaG024113 AT5G42520.1 47.899 357 126 12 1 312 1 342 8.11e-91 274
MsaG024113 AT5G42520.3 49.291 282 98 8 76 312 1 282 2.03e-77 238
MsaG024113 AT2G35550.2 45.806 155 77 1 165 312 72 226 4.90e-46 155
MsaG024113 AT2G35550.4 45.806 155 77 1 165 312 72 226 4.90e-46 155
MsaG024113 AT2G35550.3 45.806 155 77 1 165 312 72 226 4.90e-46 155
MsaG024113 AT2G35550.1 45.806 155 77 1 165 312 117 271 7.49e-46 156
MsaG024113 AT1G14685.1 52.137 117 56 0 196 312 163 279 4.08e-44 152
MsaG024113 AT1G14685.4 52.137 117 56 0 196 312 163 279 4.08e-44 152
MsaG024113 AT1G14685.3 52.137 117 56 0 196 312 163 279 4.08e-44 152
MsaG024113 AT1G14685.2 52.137 117 56 0 196 312 163 279 4.08e-44 152
MsaG024113 AT1G68120.1 55.769 104 46 0 209 312 166 269 1.20e-41 145
MsaG024113 AT2G01930.2 41.830 153 83 1 166 312 131 283 3.85e-41 144
MsaG024113 AT2G01930.1 41.830 153 83 1 166 312 131 283 3.85e-41 144

Find 86 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTACCAAAATAAAGAAAA+TGG 0.208626 4:+95950897 MsaT024113.1:CDS
CTTCTGTCATGTTGGATATA+TGG 0.216505 4:-95950756 None:intergenic
ATCTATACCATTGGATCTTA+AGG 0.242442 4:+95951239 MsaT024113.1:CDS
TCAGAAGTTATAACAGTAAC+TGG 0.272157 4:-95950820 None:intergenic
CATTGGATCTTAAGGATTAC+TGG 0.283657 4:+95951247 MsaT024113.1:CDS
TTTGTTCCTTTATTTGATGC+TGG 0.301136 4:-95950453 None:intergenic
ACTCCAGTACACGTGCAAAC+TGG 0.357093 4:-95951033 None:intergenic
TTGTTCCTTTATTTGATGCT+GGG 0.361945 4:-95950452 None:intergenic
AAATGGGCGAGGATTTAAAT+AGG 0.362648 4:+95950914 MsaT024113.1:CDS
GTAACGATTTGTTCCATGTC+TGG 0.409057 4:-95951271 None:intergenic
GTCCCAGTTTGCACGTGTAC+TGG 0.410401 4:+95951030 MsaT024113.1:CDS
TCATCTAACTAAGAGAATAA+AGG 0.412472 4:+95950855 MsaT024113.1:CDS
CGTTGAATTCCACCATTGAA+TGG 0.413813 4:-95950703 None:intergenic
CAGTACTATTCCGACTTTGA+AGG 0.424329 4:-95950672 None:intergenic
ATGCGAGCATGGCGCTTGTT+CGG 0.428637 4:-95951144 None:intergenic
TGCCTGCCTTTCAGCCATAA+TGG 0.435049 4:-95950506 None:intergenic
ACCACGACAGTGCTACAAAT+GGG 0.437083 4:+95951056 MsaT024113.1:CDS
AAGCGCCATGCTCGCATTGG+AGG 0.439433 4:+95951150 MsaT024113.1:CDS
GGATCCCCAGCATCAAATAA+AGG 0.448615 4:+95950447 MsaT024113.1:CDS
GGATAATGCTCTACTGGAAC+GGG 0.450868 4:+95950633 MsaT024113.1:CDS
CTCCAGTACACGTGCAAACT+GGG 0.457013 4:-95951032 None:intergenic
ACTGGCAACTTTCCATTCAA+TGG 0.459914 4:+95950691 MsaT024113.1:CDS
ATCTGAAAAGAATGAAGCCC+TGG 0.461756 4:+95950561 MsaT024113.1:CDS
GACAAAGATCAAATCTGAAT+GGG 0.471460 4:+95950954 MsaT024113.1:CDS
GTGCTTCATCCCTCTGCCGA+AGG 0.475401 4:-95950600 None:intergenic
CAAATCTGAATGGGATAGGC+AGG 0.475987 4:+95950963 MsaT024113.1:CDS
GTCCATTATGGCTGAAAGGC+AGG 0.476045 4:+95950504 MsaT024113.1:CDS
CCATGATTTATCTATACCAT+TGG 0.476445 4:+95951230 MsaT024113.1:CDS
GGACAAAGATCAAATCTGAA+TGG 0.478623 4:+95950953 MsaT024113.1:CDS
TGGATATATGGTTTGAAGAA+TGG 0.483617 4:-95950744 None:intergenic
AACAAGCGCCATGCTCGCAT+TGG 0.488361 4:+95951147 MsaT024113.1:CDS
TCCGCCCTCCAATGCGAGCA+TGG 0.489587 4:-95951155 None:intergenic
CCTACCAAAATAAAGAAAAT+GGG 0.492634 4:+95950898 MsaT024113.1:CDS
TGAAGAATGGTGCATCCGCT+TGG 0.493558 4:-95950731 None:intergenic
TACCACGACAGTGCTACAAA+TGG 0.496139 4:+95951055 MsaT024113.1:CDS
AATAGGAAGGCTTATTCTGA+AGG 0.504268 4:+95950931 MsaT024113.1:CDS
CTCAGTAGGTTTGCATCAGA+AGG 0.511971 4:+95951207 MsaT024113.1:CDS
TGAAAACGGTAGGCATAAGA+TGG 0.514492 4:+95950295 MsaT024113.1:CDS
AGCGCCATGCTCGCATTGGA+GGG 0.517752 4:+95951151 MsaT024113.1:CDS
TCAGAGGGATAATGCTCTAC+TGG 0.518249 4:+95950627 MsaT024113.1:CDS
GGGCTACGTCTCGAGCAGCC+AGG 0.518686 4:-95950579 None:intergenic
CTTCAAAGTCGGAATAGTAC+TGG 0.519466 4:+95950673 MsaT024113.1:CDS
TGTTCCTTTATTTGATGCTG+GGG 0.520374 4:-95950451 None:intergenic
AGTACTATTCCGACTTTGAA+GGG 0.520694 4:-95950671 None:intergenic
GGATGAAGCACTTGCTCAGA+GGG 0.522036 4:+95950612 MsaT024113.1:CDS
ATATGCTGCTTCTGTCATGT+TGG 0.532878 4:-95950764 None:intergenic
AGATCAAATCTGAATGGGAT+AGG 0.533258 4:+95950959 MsaT024113.1:CDS
TTGTTCGGAAGCTGTGGTAG+TGG 0.541295 4:-95951129 None:intergenic
CAAAATAAAGAAAATGGGCG+AGG 0.545395 4:+95950903 MsaT024113.1:CDS
GAGACGTAGCCCTTCGGCAG+AGG 0.546456 4:+95950590 MsaT024113.1:CDS
CCAATGGTATAGATAAATCA+TGG 0.547636 4:-95951230 None:intergenic
GGCTACGTCTCGAGCAGCCA+GGG 0.552588 4:-95950578 None:intergenic
CCCCATTTGTAGCACTGTCG+TGG 0.555932 4:-95951057 None:intergenic
CAGAAGTTATAACAGTAACT+GGG 0.557616 4:-95950819 None:intergenic
TTTAAATGTGCAGTGGAATG+TGG 0.558628 4:+95950426 MsaT024113.1:intron
CTGCTCGAGACGTAGCCCTT+CGG 0.563490 4:+95950584 MsaT024113.1:CDS
CTACAGAGGAGCACATTCCC+TGG 0.565304 4:+95950322 MsaT024113.1:CDS
AGACGTAGCCCTTCGGCAGA+GGG 0.565616 4:+95950591 MsaT024113.1:CDS
GGGCGAGGATTTAAATAGGA+AGG 0.570874 4:+95950918 MsaT024113.1:CDS
CTCTTGCAGCCCTTCAAAGT+CGG 0.574408 4:+95950662 MsaT024113.1:CDS
AAGGATTACTGGGCCAGACA+TGG 0.576589 4:+95951258 MsaT024113.1:CDS
GCCATGCTCGCATTGGAGGG+CGG 0.577730 4:+95951154 MsaT024113.1:CDS
TGCTTCATCCCTCTGCCGAA+GGG 0.577861 4:-95950599 None:intergenic
GGGATAATGCTCTACTGGAA+CGG 0.583003 4:+95950632 MsaT024113.1:CDS
GTACACGTGCAAACTGGGAC+AGG 0.585669 4:-95951027 None:intergenic
ATAGGAAGGCTTATTCTGAA+GGG 0.587900 4:+95950932 MsaT024113.1:CDS
ATTGGATCTTAAGGATTACT+GGG 0.588784 4:+95951248 MsaT024113.1:CDS
TCTTCTTGAACTTGAACTAG+AGG 0.590105 4:+95950531 MsaT024113.1:CDS
TTAGGTCCATTATGGCTGAA+AGG 0.590519 4:+95950500 MsaT024113.1:CDS
GATGACCGCCAATATGAAAA+CGG 0.592001 4:+95950281 MsaT024113.1:CDS
GAATGGGATAGGCAGGATGT+TGG 0.593838 4:+95950970 MsaT024113.1:CDS
CAGTAATCCTTAAGATCCAA+TGG 0.595456 4:-95951246 None:intergenic
GGCAACTTTCCATTCAATGG+TGG 0.607937 4:+95950694 MsaT024113.1:CDS
TGGCGCTTGTTCGGAAGCTG+TGG 0.609294 4:-95951135 None:intergenic
CCACGACAGTGCTACAAATG+GGG 0.618535 4:+95951057 MsaT024113.1:CDS
CATAAGATGGACTACTACAG+AGG 0.628336 4:+95950308 MsaT024113.1:CDS
ATTGGAGGGCGGAAAATGAG+CGG 0.633747 4:+95951165 MsaT024113.1:CDS
CACGACAGTGCTACAAATGG+GGG 0.651085 4:+95951058 MsaT024113.1:CDS
ACCGCCAATATGAAAACGGT+AGG 0.665966 4:+95950285 MsaT024113.1:CDS
GGGATGAAGCACTTGCTCAG+AGG 0.669930 4:+95950611 MsaT024113.1:CDS
AGTTATAACAGTAACTGGGG+AGG 0.670600 4:-95950815 None:intergenic
AGAAGTTATAACAGTAACTG+GGG 0.682187 4:-95950818 None:intergenic
TGGATGCATTGACAATGTAG+TGG 0.702687 4:-95951109 None:intergenic
GAATTCAACGTGGATCCAAG+CGG 0.708406 4:+95950716 MsaT024113.1:CDS
TTCAATGGTGGAATTCAACG+TGG 0.712678 4:+95950706 MsaT024113.1:CDS
AGAAGCAGCATATAGCACGA+CGG 0.752304 4:+95950774 MsaT024113.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr4 gene 95950275 95951308 95950275 ID=MsaG024113
Chr4 mRNA 95950275 95951308 95950275 ID=MsaT024113.1;Parent=MsaG024113
Chr4 exon 95950275 95950343 95950275 ID=MsaT024113.1.exon1;Parent=MsaT024113.1
Chr4 CDS 95950275 95950343 95950275 ID=cds.MsaT024113.1;Parent=MsaT024113.1
Chr4 exon 95950439 95951308 95950439 ID=MsaT024113.1.exon2;Parent=MsaT024113.1
Chr4 CDS 95950439 95951308 95950439 ID=cds.MsaT024113.1;Parent=MsaT024113.1
Gene Sequence

>MsaT024113.1

ATGGACGATGACCGCCAATATGAAAACGGTAGGCATAAGATGGACTACTACAGAGGAGCACATTCCCTGTGGAATGTGGATCCCCAGCATCAAATAAAGGAACAAAATGCCCTAGTTATGAATAAAAAAATTAGGTCCATTATGGCTGAAAGGCAGGCAGCTCTTCTTGAACTTGAACTAGAGGCTGCTATATCTGAAAAGAATGAAGCCCTGGCTGCTCGAGACGTAGCCCTTCGGCAGAGGGATGAAGCACTTGCTCAGAGGGATAATGCTCTACTGGAACGGGACAATGCTCTTGCAGCCCTTCAAAGTCGGAATAGTACTGGCAACTTTCCATTCAATGGTGGAATTCAACGTGGATCCAAGCGGATGCACCATTCTTCAAACCATATATCCAACATGACAGAAGCAGCATATAGCACGACGGATATAATAATAAGAGATGCCTCCCCAGTTACTGTTATAACTTCTGAAGATGTCAAATCTCATCTAACTAAGAGAATAAAGGAGAACAAAGCATCACAGACACCTACCAAAATAAAGAAAATGGGCGAGGATTTAAATAGGAAGGCTTATTCTGAAGGGACAAAGATCAAATCTGAATGGGATAGGCAGGATGTTGGCTTGAATTTGATTGCTTTCGATGAAACTGTGATGCCTGTCCCAGTTTGCACGTGTACTGGAGTACCACGACAGTGCTACAAATGGGGGAATGGTGGATGGCAATCATCTTGTTGTACCACTACATTGTCAATGCATCCACTACCACAGCTTCCGAACAAGCGCCATGCTCGCATTGGAGGGCGGAAAATGAGCGGCAATGTTTTTAGACGACTTCTCAGTAGGTTTGCATCAGAAGGCCATGATTTATCTATACCATTGGATCTTAAGGATTACTGGGCCAGACATGGAACAAATCGTTACATTACTATCAAGTAG
Protein sequence

>MsaT024113.1

MDDDRQYENGRHKMDYYRGAHSLWNVDPQHQIKEQNALVMNKKIRSIMAERQAALLELELEAAISEKNEALAARDVALRQRDEALAQRDNALLERDNALAALQSRNSTGNFPFNGGIQRGSKRMHHSSNHISNMTEAAYSTTDIIIRDASPVTVITSEDVKSHLTKRIKENKASQTPTKIKKMGEDLNRKAYSEGTKIKSEWDRQDVGLNLIAFDETVMPVPVCTCTGVPRQCYKWGNGGWQSSCCTTTLSMHPLPQLPNKRHARIGGRKMSGNVFRRLLSRFASEGHDLSIPLDLKDYWARHGTNRYITIK*